DE19728520A1 - Switchable dynamic micromixer with minimal dead volume - Google Patents

Switchable dynamic micromixer with minimal dead volume

Info

Publication number
DE19728520A1
DE19728520A1 DE1997128520 DE19728520A DE19728520A1 DE 19728520 A1 DE19728520 A1 DE 19728520A1 DE 1997128520 DE1997128520 DE 1997128520 DE 19728520 A DE19728520 A DE 19728520A DE 19728520 A1 DE19728520 A1 DE 19728520A1
Authority
DE
Germany
Prior art keywords
mixing chamber
micromixer
discharge
channels
switchable
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
DE1997128520
Other languages
German (de)
Inventor
Geb Schmidt Schmidt
John S Prof Dr Rer N Mccaskill
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
IMB INST fur MOLEKULARE BIOTE
Original Assignee
IMB INST fur MOLEKULARE BIOTE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by IMB INST fur MOLEKULARE BIOTE filed Critical IMB INST fur MOLEKULARE BIOTE
Priority to DE1997128520 priority Critical patent/DE19728520A1/en
Publication of DE19728520A1 publication Critical patent/DE19728520A1/en
Ceased legal-status Critical Current

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
    • B01F13/00Other mixers; Mixing plant, including combinations of mixers, e.g. of dissimilar mixers
    • B01F13/08Magnetic mixers ; Mixers having magnetically driven stirrers
    • B01F13/0818Magnetic mixers ; Mixers having magnetically driven stirrers using independent floating stirring elements
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
    • B01F13/00Other mixers; Mixing plant, including combinations of mixers, e.g. of dissimilar mixers
    • B01F13/0059Micromixers

Abstract

The invention relates to a switchable dynamic micromixer with minimum dead volume used for cyclic or continuous mixing of small fluids ranging from 1 nl to 10 mu l. The invention provides for a micromixer which is brought into contact on one side with at least two feed ducts (21, 22) and which has at least one discharge duct (26) opposite the feed ducts (21, 22). The micromixer also has a mixing chamber (23) with several magnetizable pearls (4) which are covered on one side by a lid (30) and which can move freely. The entire length of the pearls arranged in an adjacent line is slightly lower than the smallest lateral dimension of the mixing chamber. A magnetic system (5) can be set into rotation and assigned and switched in such a way that it enables an adjacent linear arrangement of the magnetizable pearls (4) in addition to joint rotation of the linear pearl structure.

Description

The invention relates to a switchable dynamic micromixer minimal dead volume, the cyclical or continuous Mix the smallest amounts of liquid in the order of 1 nl to 10 µl serves. The micromixer is preferred, especially in connection with several micromixers with each other, in which Biotechnology, medical diagnostics, for pharmaceutical Screening or DNA computing.

Devices for homogenizing are known from the prior art of liquids in the form of dynamic and static Known micromixers.

Use static micromixers, e.g. B. in MST-news 19/97 p. 30-31 (ISSN 09483128) describes the diffusion for homogenizing Solutions when using long contact paths and small ones Channel diameters. The disadvantages of this mixed variant are due to the necessarily long flow channels in the resulting pressure losses in the flow system, the low Efficiency of the mixing process, the relatively large Dead volume and the relatively long mixing times.

DE 195 11 603 A1 describes an arrangement for static mixing described, which achieves a shortening of the diffusion paths by that two or more liquids are divided several times and layer by layer. This also works Mixing of insoluble fluids. The dead volume is also here the mixing device, caused by repeated rerouting and Layering the liquids, very large and the mixing times also very long.

Another static micro-mixer is described in DE 44 16 343 C2 described. According to this proposal, several are mixed Solutions are also diffusive, with the fluids to be mixed before Mixing chamber made of plate-like, stacked elements are composed of the oblique to the micro-mixer longitudinal axis extending channels are crossed, and being the channels neighboring elements cross without contact and into the  Mixing chamber open. Because here too the mixing effect by diffusion A disadvantage of this arrangement is the long mixing time for complete homogenization.

Dynamic mixers use rotating mixing tools that Mixing energy into the mix to homogenize the mix Bring components. Because of the constructive, relative Large volume versions of these mixers are not for mixing smallest amounts of liquid suitable for the one hand intended use of the present invention is not required be or, e.g. B. for cost reasons, are not provided can. A microflow processor closest to the invention is described in EP 0495 255 A1. With this microflow processor mixing small amounts of samples with the smallest possible Dead volume sought, with flow rates in the range of ml / min until oil / min can be operated. Part of this microflow processor is a micromixer that is minimal, due to its no further increase increasing miniaturization, can have a volume of 0.1 µl.

The invention has for its object to a micromixer create the two or more liquids in very small Volumes, preferably in a range below 100 nl, are present in very short time, with low dead volume and high efficiency mixed, can make the interruption interruptible if necessary and the integration of multiple micromixers within one Basic body allows.

The task is characterized by the distinctive features of the first Claim resolved. Advantageous refinements are due to the subordinate claims recorded.

The invention is based on a schematic embodiment examples will be explained in more detail. Show it:  

FIG. 1a is a first possible embodiment of a micro mixer in the assembled state without a filling of, media to be mixed

FIG. 1b is a micro-mixer of FIG. 1 with the filling media to be mixed,

Fig. 2a shows a second possible embodiment of a micro mixer in the assembled state without a filling of, media to be mixed

FIG. 2b shows a micro-mixer of FIG. 2 with the filling media to be mixed,

Fig. 3a shows a third possible embodiment of a micro mixer in the assembled condition with the refilling, media to be mixed and active mixing element

FIG. 3b, the micro-mixer according to Fig. 3a, which is traversed by two laminar flowing media at rest position of the mixing element,

Fig. 4 shows an interconnection of three micromixers according to Fig. 1 and

Fig. 5 shows a preferred embodiment of a discharge channel adjoining a mixing chamber.

Fig. 1 shows a first possible embodiment of a micro-mixer 1 according to the present invention. In the example, the micromixer 1 is formed from a first base plate 20 , into which a mixing chamber 23 and two feed channels 21 and 22 adjoining the mixing chamber 23 are introduced. On the side opposite the feed channels 21 , 22 , the mixing chamber 23 is followed by comb-shaped capillary paths 24 which open into a trench 25 to which a discharge channel 26 is connected. A plurality of magnetizable beads 4 , in particular made of a ferromagnetic material, are also introduced into the mixing chamber 23 . The diameter of these beads 4 is such that it lies somewhat below the clear chamber height limited by a cover plate 30 . A rotatable magnet 5 (see FIG. 1b) is provided below the base plate 20 . With a corresponding determination of its magnetic polarization, this magnet 5 brings about a linear and adjacent alignment of the beads 4 , which, following the magnetic rotation, experience a rotation within the mixing chamber 23 . Depending on the predetermined volume of the mixing chamber 23 , the diameter of the beads can be between 1 μm and 100 μm. Their total number is then further determined so that the length of the linearly aligned pearl structure is below the smallest lateral extent of the mixing chamber 23 .

The mixing chamber 23 , the feed channels 21 , 22 , the discharge channel 26 , the comb-shaped capillary paths 24 and the trench 25 are introduced into the base body 20 with the aid of microstructuring technologies. Both wet chemical or physical etching techniques for structuring silicon or photo-structurable glass, laser structuring processes or molding techniques for polymers can be used to produce the structures. The base body 20 , which carries the structures thus produced, is sealed with a cover plate 30 , consisting of a glass or a transparent polymer. This means that the mixing result in the mixing chamber or in the subsequent channels can be detected at any time. The beads 4 can be introduced before the base body 20 is closed with the cover plate 30 or at a later point in time when the beads 4 are pumped into the mixing chamber 23 together with a liquid, with the feed channels 21 , 22 appropriately designed . A return transport of the beads 4 from the mixing chamber 23 is prevented by a flow flow maintained in the micromixer. If the beads 4 are finally brought into the mixing chamber 23 , they are demagnetized before being transported into the mixing chamber 23 in order to avoid clogging due to a plurality of beads 4 being connected. When an external magnetic field is switched on for the first time, the beads 4 are magnetized and only then exhibit a ferromagnetic behavior. This leads to the fact that, due to the ferromagnetic material of the beads 4 , a plurality of beads 4 always come together and join together to form the chain-shaped structure shown, and then rotate together when a position-changing magnetic field is applied. This requires a stirring effect with a high degree of mixing, as indicated in Fig. 1b. There, two fluid media A and B are passed through the feed channels 21 , 22 into the mixing chamber 23 , in which, in the illustration, the mixing of the linearly oriented pearl structure has already optimally mixed them. The mixed medium C can then be discharged via the comb-shaped capillary paths 24 , the trench 25 and the discharge channel 26 . The design of the capillary paths 24 , which adjoin the mixing chamber 23 , each with an opening cross-section that is smaller than the diameter of the pearls 4 used , represents an effective retention means for the pearls 4. It is within the scope of the invention to provide further discharge channels 26 on the trench 25 for deriving the identical mixing result C. In the embodiment of the micromixer 1 according to FIGS . 1a, 1b, the fluids A, B to be mixed are permanently mixed with one another. The discharge channel can also be used as a detection channel, for which purpose a particularly preferred embodiment is described in FIG. 5. For conventional uses of the micromixer 1 , such as in molecular biology, the mixing chamber 23 is given a volume of 1 nl to 10 μl.

FIGS. 2a and 2b basically describe an identical design to FIGS. 1a and 1b; Identical functional elements are provided with the same reference symbols. The only difference is that here the retaining means for the beads 4 is formed by an overflow channel 24 '. In its width dimension b, this overflow channel 24 ′ extends essentially over the width of the mixing chamber 23 , to which it adjoins. The gap-shaped vertical extension of the overflow channel 24 ', which is bounded at the top by the adjoining cover plate 30 , is dimensioned in such a way that the beads 4 cannot get into the overflow channel 24 ' in comparison with the bead diameters used.

The micromixers 1 designed according to FIGS . 1a, 1b, 2a, 2b are designed for purely dynamic operation, that is to say for the constant mixing of fluids. These designs of the micromixers 1 have a plurality, at least two, of inputs 21 and 22 , which in this mode of operation do not necessarily have to lie in one plane with the other components, such as 23 and the following ones, via which the solutions A and B of the mixing chamber 23 can be supplied, are mixed there by means of the beads 4 in the manner described, so that a mixture C can be removed from the discharge channel 26 .

The micromixer 1 undergoes a certain modification for further uses, as indicated in FIGS . 3a and 3b. These versions represent a switchable micromixer. If the micromixer 1 according to FIGS . 1a, 1b, 2a, 2b contained only one discharge channel 26 , three discharge channels 26 , 27 , 28 are provided in an embodiment according to FIG. 3a, with their height dimensioning the height dimensioning of the overflow channel 24 'of FIG. 2a are carried out similarly, so that the channel edge formations 261, 271, 281 at the same time the function of the overflow channel 24' take over.

Fig. 3a shows the case that the micro mixer in dynamic operation analogous works to the previous figures, and thus via the supply channels 21, 22 supplied liquid media A and B are mixed. In this mode of operation, an identical mixed solution C can be taken from all discharge channels 26 , 27 , 28 .

Provided that there are laminar flow conditions in the mixing chamber 23 , which can be achieved by adhering to Reynold numbers <1, the discharge channels 26 , 27 , 28 are assigned with the proviso that the two discharge channels 21 , 22 provided in the example are the three discharge channels 26 , 27 , 28 are assigned at the other end of the mixing chamber in such a way that the first feed channel 21 , and thus the first medium A that can be fed through it, a first discharge channel 27 , the second feed channel 22 , and thus the second medium B that can be fed through this a second discharge duct 28 and a third discharge duct 26 , the contents of which are subsequently discarded, are assigned to a common throughflow zone formed by the media A and B. If the micromixer in the example according to FIG. 3b works in static operation, ie the beads 4 are not exposed to the rotating magnetic field, and if a laminar flow through the mixing chamber 23 is ensured, a relatively sharp interface is formed between the two media flows A and B. This interface zone S and its closely adjacent areas are taken up by the discharge channel 26 , as a result of which contamination of the individual components A and B is avoided, and the pure media stream of component A reaches the discharge channel 27 and that of component B enters the discharge channel 28 . The mixed media stream W is usually discarded in the further process. A micromixer manufactured according to FIGS . 3a and 3b can be expanded within the scope of the invention to a plurality of feed and discharge channels, whereby the above requirements are to be observed in each case and a further channel for a partially mixed component W of the respective border zone area is to be provided between two channels which purely purge components . Between the operational states of FIG. 3a and FIG. 3b may be mutually connected, which for example. For a combinatorial processing of many components is beneficial and z. B. allows synthesis in the river.

The micromixers described in FIGS . 1a to 3b can be connected in series in any number, as a result of which entire networks of mixing agents are possible. Such a design is shown in FIG. 4 using three micromixers 1 a, 1 b, 1 c designed according to FIG. 1. Each of these micromixers contains a mixing chamber 23 a, 23 b, 23 c. In this example it is also possible, depending on the desired process sequence, to block individual or multiple feed channels if necessary, so that only one or no components of a micromixer get into the other micromixers. When using the above-mentioned structuring methods for the production of the micromixers, several micromixers can be accommodated in one piece in a base body 20 and covered by a common cover plate 30 . In a practical implementation variant, for example, if necessary, up to 90,000 individual mixing chambers 23 and the associated feed and discharge channels can be introduced into a 4 '' silicon wafer if the mixing chambers 23 comprise a cavity of 100,100.50 μm 3 .

Finally, FIG. 5 shows a special design of a discharge channel 26 , in particular for the last-mentioned, but not limited to, the integrated embodiment variant. This discharge channel connects, analogous to that shown in FIGS . 1a to 3b, on the one hand to the mixing chamber or subordinate assemblies and is designed to be meandered several times over a length adapted to the mixing chamber volume. In this case, the volume of the channel 26 should be dimensioned such that it takes up at least three times the volume of the mixing chamber volume. This meandering design of the discharge channel favors the use of commercially available detection units, for example optical spectroscopes, with the aid of which a relatively large sample volume and thus increased signals are available when imaged by a transparent cover plate 30 , since several meandering channel sections can be detected at the same time.

All the described embodiments have an extraordinary low dead volume, since practically everything Mixing chamber volume can be supplied for further uses.

For all of the exemplary embodiments described, it is expressly within the scope of the invention that the structures introduced into the base body 20 are also introduced into the cover plate 30 in a mirror-image identical manner. Such training opens z. B. reference to Figs. 1a and 1b, with appropriate choice of materials for the base body and the cover plate, for example. Pyrex glass, a circular cross-sectional configuration of the capillaries 24 which then symmetrically lying to the mixing chamber 23, also with the provision, not shown, magnetic systems, the positioning of individual beads 4 enable a switchable closure of the drainage path in individual or all capillary mouth areas.

All in the description, the following claims and the Features shown in the drawing can be used both individually and in any combination with each other be essential to the invention.  

Reference list

1

,

1

a,

1

b,

1

cMicromic

20th

Basic body

21

,

22

Feed channels

23

Mixing chamber

24th

comb-like capillary paths

24th

'' Overflow channel

25th

Receiving trench

26

,

27

,

28

Discharge channels

261

a,

261

b,

261

c Discharge / feed channels

30th

Cover plate

4th

magnetizable beads

5

switchable, rotatable magnet system
A, B, C, W fluid media

Claims (16)

1. Switchable dynamic micromixer with minimal dead volume, comprising a mixing chamber ( 23 ) which is connected on one side to at least two supply channels ( 21 , 22 ) and on the other hand, opposite to the supply channels ( 21 , 22 ), has at least one discharge channel ( 26 ) , Several magnetizable beads ( 4 ) are provided within the mixing chamber ( 23 ), the diameter of which is somewhat smaller than the inside chamber height of the mixing chamber ( 23 ) so that they are within the mixing chamber walls, which are covered on one side by a cover ( 30 ) , can move freely, and the total length of which is defined in a linear, mutually adjacent arrangement somewhat below the smallest lateral mixing chamber extent, retention means ( 24 ; 24 '; 251-271 ) are provided between the mixing chamber outlet and the entrance of the at least one discharge channel ( 26 ), the penetration of the beads ( 4 ) into the discharge channel ( 26 )) or the discharge Prevent channels ( 251-271 ) and the mixing chamber ( 23 ) can be assigned a rotatable magnet system ( 5 ), which is designed so that it enables a linear adjacent line up of the magnetizable beads ( 4 ) and a common rotation of the linear bead structure .
2. Switchable dynamic micromixer according to claim 1, characterized in that it consists of a first base body ( 20 ) in which the structures for the mixing chamber ( 23 ), the at least two feed channels ( 21 , 22 ), the at least one discharge channel ( 26 ) and the retaining means ( 24 ; 24 '; 251-271 ) are introduced, which is covered on one side by a second cover plate ( 30 ).
3. Switchable dynamic micromixer according to claim 2, characterized in that the same structures ( 23 ; 21 , 22 ; 23 ; 24 ; 24 ';251-271; 26 ) of the base body ( 20 ) are introduced in mirror image in the cover plate ( 30 ) are.
4. Switchable dynamic micromixer according to claim 1 or 2, characterized in that the retaining means are formed by a multiplicity of comb-shaped capillary paths ( 24 ) which, on the one hand, connect to the mixing chamber ( 23 ), each with an opening cross section which is smaller than the diameter of one Pearl ( 4 ) and on the other hand open into a receiving trench ( 25 ) to which the at least one discharge channel ( 26 ) connects.
5. Switchable dynamic micromixer according to claim 1 or 2, characterized in that the retaining means are formed by an overflow channel ( 24 ') adjoining the mixing chamber ( 23 ) in a step-like manner, which on the other hand opens into a receiving trench ( 25 ) to which the at least one discharge channel ( 26 ) connects.
6. Switchable dynamic micromixer according to claim 5, characterized in that the width (b) of the overflow channel ( 24 ') corresponds essentially to the adjacent mixing chamber extension.
7. Switchable dynamic micromixer according to claim 1 or 2, characterized in that two respective feed channels ( 21 , 22 ) are assigned three associated discharge channels ( 26 , 27 , 28 ) in such a way that, with essentially laminar flow through the mixing chamber ( 23 ), the first feed channel ( 21 ), and thus the first medium (A) that can be fed through it, a first discharge channel ( 27 ), the second feed channel ( 22 ), and thus the second medium (B) that can be fed through this, a second discharge channel ( 28 ) and a third discharge channel ( 26 ) is assigned to a common throughflow zone (S) that can be formed by the media (A, B).
8. Switchable dynamic micromixer according to claim 7, characterized in that the discharge channels ( 26 , 27 , 28 ) formed step-like to the mixing chamber ( 23 ) connect such that their smallest lateral dimensions below the diameter of the beads ( 4 ) are fixed.
9. Switchable dynamic micromixer according to one of the preceding claims, characterized in that a plurality of micromixers ( 1 a, 1 b, 1 c) are associated with one another in such a way that in each case at least two discharge channels ( 261 a, 261 b), each of which Discharge channel ( 261 a, 261 b) belongs to a separate mixing chamber ( 1 a, 1 b), which form feed channels to another mixing chamber ( 1 c).
10. Switchable dynamic micromixer according to claim 9, characterized characterized in that when using several interconnected Micromixer one or more feed or discharge channels optional are lockable.
11. Switchable dynamic micromixer according to one of the preceding claims, characterized in that the at least in the base body ( 20 ) recesses for the individual functional modules ( 21 , 22 ; 23 ; 24 ; 24 ', 26-27 ) by wet chemical etching techniques or physical Removal processes or by impression techniques are introduced.
12. Switchable dynamic micromixer according to one of the preceding claims, characterized in that the base body ( 20 ) with the structures introduced therein for the individual functional modules ( 21 , 22 ; 23 ; 24 ; 24 ', 26-27 ) is formed in one piece.
13. Switchable dynamic micromixer according to claim 12, characterized in that in the one-piece base body ( 20 ) a plurality of micromixers ( 1 a, 1 b, 1 c) are introduced.
14. Switchable dynamic micromixer according to one of the preceding claims, characterized in that at least one discharge channel ( 26 , 27 , 28 , 261 a, 261 b) is formed in a meandering manner over a region of its lateral extent.
15. Switchable dynamic micromixer according to claim 14, characterized in that the individual parallel meandering sections are introduced as close as possible to one another in the base body ( 20 ) and / or the cover plate ( 30 ).
16. Switchable dynamic micromixer according to claim 14, characterized in that the multiply meandered discharge duct sections are able to take up a volume which corresponds to at least three times the volume of the volume comprised by the mixing chamber ( 23 ).
DE1997128520 1997-07-04 1997-07-04 Switchable dynamic micromixer with minimal dead volume Ceased DE19728520A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE1997128520 DE19728520A1 (en) 1997-07-04 1997-07-04 Switchable dynamic micromixer with minimal dead volume

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE1997128520 DE19728520A1 (en) 1997-07-04 1997-07-04 Switchable dynamic micromixer with minimal dead volume
PCT/EP1998/003942 WO1999001209A1 (en) 1997-07-04 1998-06-27 Switchable dynamic micromixer with minimum dead volume

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Cited By (10)

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DE19917148A1 (en) * 1999-04-16 2000-10-26 Inst Mikrotechnik Mainz Gmbh Production of dispersion from fluids using a micro-mixer splitting fluid flows into lamellae and combining them to cause fragmentation into droplets forming dispersion, assisted by piezoelectrically-generated mechanical oscillations
WO2001043871A2 (en) * 1999-12-06 2001-06-21 Incyte Genomics, Inc. Microarray hybridization chamber
US6513968B2 (en) 1998-08-21 2003-02-04 Agilent Technologies, Inc. Apparatus and method for mixing a film of fluid
US6911343B2 (en) 1999-06-30 2005-06-28 Agilent Technologies, Inc. Method for conducting chemical or biochemical reactions on a solid surface within an enclosed chamber
EP1586372A1 (en) * 2004-03-19 2005-10-19 Goldschmidt GmbH Alkoxylation in a microstructured capillary reactor
WO2005121310A2 (en) * 2004-06-07 2005-12-22 Bioprocessors Corp. Creation of shear in a reactor
DE10213003B4 (en) * 2002-03-22 2006-08-03 Forschungszentrum Karlsruhe Gmbh Micromixer and method for mixing at least two liquids and using micromixers
EP2105202A1 (en) * 2008-03-28 2009-09-30 Stichting Dutch Polymer Institute Apparatus and method for a microfluidic mixer and pump
WO2009132616A2 (en) * 2008-04-29 2009-11-05 Forschungszentrum Jülich GmbH Supply system
DE202015104827U1 (en) 2015-09-11 2015-10-22 Leibniz-Institut Für Photonische Technologien E.V. Flow control module

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DE10218280C1 (en) * 2002-04-19 2003-11-20 Fraunhofer Ges Forschung Integrated mixing and switching system for microreaction technology

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US6513968B2 (en) 1998-08-21 2003-02-04 Agilent Technologies, Inc. Apparatus and method for mixing a film of fluid
US8012765B2 (en) 1998-08-21 2011-09-06 Agilent Technologies, Inc. Method for mixing a film of fluid
US7371349B2 (en) 1998-08-21 2008-05-13 Agilent Technologies, Inc. Apparatus and method for mixing a film of fluid
DE19917148C2 (en) * 1999-04-16 2002-01-10 Inst Mikrotechnik Mainz Gmbh Process and micromixer for producing a dispersion
DE19917148A1 (en) * 1999-04-16 2000-10-26 Inst Mikrotechnik Mainz Gmbh Production of dispersion from fluids using a micro-mixer splitting fluid flows into lamellae and combining them to cause fragmentation into droplets forming dispersion, assisted by piezoelectrically-generated mechanical oscillations
US7247499B2 (en) 1999-06-30 2007-07-24 Agilent Technologies, Inc. Method for conducting binding reactions on a solid surface within an enclosed chamber
US6911343B2 (en) 1999-06-30 2005-06-28 Agilent Technologies, Inc. Method for conducting chemical or biochemical reactions on a solid surface within an enclosed chamber
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WO2001043871A2 (en) * 1999-12-06 2001-06-21 Incyte Genomics, Inc. Microarray hybridization chamber
DE10213003B4 (en) * 2002-03-22 2006-08-03 Forschungszentrum Karlsruhe Gmbh Micromixer and method for mixing at least two liquids and using micromixers
EP1586372A1 (en) * 2004-03-19 2005-10-19 Goldschmidt GmbH Alkoxylation in a microstructured capillary reactor
WO2005121310A2 (en) * 2004-06-07 2005-12-22 Bioprocessors Corp. Creation of shear in a reactor
WO2005121310A3 (en) * 2004-06-07 2006-04-13 Bernardo Aumond Creation of shear in a reactor
WO2009118689A1 (en) * 2008-03-28 2009-10-01 Stichting Dutch Polymer Institute Apparatus and method for a microfluidic mixer and pump
EP2105202A1 (en) * 2008-03-28 2009-09-30 Stichting Dutch Polymer Institute Apparatus and method for a microfluidic mixer and pump
WO2009132616A2 (en) * 2008-04-29 2009-11-05 Forschungszentrum Jülich GmbH Supply system
WO2009132616A3 (en) * 2008-04-29 2010-04-15 Forschungszentrum Jülich GmbH Supply system
DE202015104827U1 (en) 2015-09-11 2015-10-22 Leibniz-Institut Für Photonische Technologien E.V. Flow control module

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