DE112019007287T5 - Multikapillar-elektrophoresevorrichtung und verfahren zur probenanalyse - Google Patents

Multikapillar-elektrophoresevorrichtung und verfahren zur probenanalyse Download PDF

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Publication number
DE112019007287T5
DE112019007287T5 DE112019007287.0T DE112019007287T DE112019007287T5 DE 112019007287 T5 DE112019007287 T5 DE 112019007287T5 DE 112019007287 T DE112019007287 T DE 112019007287T DE 112019007287 T5 DE112019007287 T5 DE 112019007287T5
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Germany
Prior art keywords
capillaries
correction index
signal intensity
sample
fluorescence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE112019007287.0T
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German (de)
English (en)
Inventor
Noriyuki Sumida
Takashi Anazawa
Michiru Fujioka
Motohiro Yamazaki
Taro Nakazawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hitachi High Tech Corp
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Hitachi High Tech Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi High Tech Corp filed Critical Hitachi High Tech Corp
Publication of DE112019007287T5 publication Critical patent/DE112019007287T5/de
Pending legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44782—Apparatus specially adapted therefor of a plurality of samples

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Optics & Photonics (AREA)
  • Biomedical Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
DE112019007287.0T 2019-06-06 2019-06-06 Multikapillar-elektrophoresevorrichtung und verfahren zur probenanalyse Pending DE112019007287T5 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2019/022449 WO2020245961A1 (ja) 2019-06-06 2019-06-06 マルチキャピラリ電気泳動装置、及びサンプル分析方法

Publications (1)

Publication Number Publication Date
DE112019007287T5 true DE112019007287T5 (de) 2022-04-14

Family

ID=73653124

Family Applications (1)

Application Number Title Priority Date Filing Date
DE112019007287.0T Pending DE112019007287T5 (de) 2019-06-06 2019-06-06 Multikapillar-elektrophoresevorrichtung und verfahren zur probenanalyse

Country Status (6)

Country Link
US (1) US20220229013A1 (https=)
JP (1) JP7261877B2 (https=)
CN (2) CN118191073A (https=)
DE (1) DE112019007287T5 (https=)
GB (1) GB2599029B (https=)
WO (1) WO2020245961A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7495999B2 (ja) * 2020-12-18 2024-06-05 株式会社日立ハイテク キャピラリアレイ

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012168138A (ja) 2011-02-17 2012-09-06 Hitachi High-Technologies Corp 電気泳動装置
JP2016176764A (ja) 2015-03-19 2016-10-06 株式会社島津製作所 キャピラリー電気泳動装置及びキャピラリー電気泳動による試料分析方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10206384A (ja) * 1997-01-16 1998-08-07 Kagaku Gijutsu Shinko Jigyodan マルチキャピラリー電気泳動装置
JP2000258392A (ja) * 1999-03-05 2000-09-22 Hitachi Ltd 電気泳動装置
JP4175735B2 (ja) * 1999-05-12 2008-11-05 独立行政法人理化学研究所 マルチキャピラリー電気泳動装置
US6982029B2 (en) * 2001-05-07 2006-01-03 Spectramedix Llc Electrophoretic method and system having internal lane standards for color calibration
US8012327B2 (en) * 2006-01-16 2011-09-06 Hitachi High-Technologies Corporation Capillary electrophoresis apparatus and electrophoresis method
CN102411024B (zh) * 2011-06-15 2014-07-09 公安部第一研究所 一种基于空间校正及光谱校正的毛细管阵列电泳检测方法
JP6087128B2 (ja) * 2012-12-17 2017-03-01 株式会社日立ハイテクノロジーズ 遺伝子型解析装置及び遺伝子型解析方法
JP6113549B2 (ja) * 2013-03-28 2017-04-12 株式会社日立ハイテクノロジーズ 電気泳動装置
SG11201908723XA (en) * 2017-02-24 2019-10-30 Life Technologies Corp Optical systems and methods for sample separation

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012168138A (ja) 2011-02-17 2012-09-06 Hitachi High-Technologies Corp 電気泳動装置
JP2016176764A (ja) 2015-03-19 2016-10-06 株式会社島津製作所 キャピラリー電気泳動装置及びキャピラリー電気泳動による試料分析方法

Also Published As

Publication number Publication date
GB202117085D0 (en) 2022-01-12
GB2599029B (en) 2023-05-17
JP7261877B2 (ja) 2023-04-20
CN113939733A (zh) 2022-01-14
US20220229013A1 (en) 2022-07-21
CN118191073A (zh) 2024-06-14
CN113939733B (zh) 2024-05-14
JPWO2020245961A1 (https=) 2020-12-10
GB2599029A (en) 2022-03-23
WO2020245961A1 (ja) 2020-12-10

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