DE102017219177A1 - System and method for monitoring a vibration transmitting unit, in particular a sonotrode for ultrasonic lysis - Google Patents

System and method for monitoring a vibration transmitting unit, in particular a sonotrode for ultrasonic lysis

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Publication number
DE102017219177A1
DE102017219177A1 DE102017219177.5A DE102017219177A DE102017219177A1 DE 102017219177 A1 DE102017219177 A1 DE 102017219177A1 DE 102017219177 A DE102017219177 A DE 102017219177A DE 102017219177 A1 DE102017219177 A1 DE 102017219177A1
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Germany
Prior art keywords
system
mechanical vibrations
device
transmitting unit
particular
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE102017219177.5A
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German (de)
Inventor
Philipp Jung
Christoph Faigle
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Robert Bosch GmbH
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Robert Bosch GmbH
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Filing date
Publication date
Application filed by Robert Bosch GmbH filed Critical Robert Bosch GmbH
Priority to DE102017219177.5A priority Critical patent/DE102017219177A1/en
Publication of DE102017219177A1 publication Critical patent/DE102017219177A1/en
Application status is Pending legal-status Critical

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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B06GENERATING OR TRANSMITTING MECHANICAL VIBRATIONS IN GENERAL
    • B06BMETHODS OR APPARATUS FOR GENERATING OR TRANSMITTING MECHANICAL VIBRATIONS OF INFRASONIC, SONIC, OR ULTRASONIC FREQUENCY, e.g. FOR PERFORMING MECHANICAL WORK IN GENERAL
    • B06B1/00Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency
    • B06B1/02Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency making use of electrical energy
    • B06B1/0207Driving circuits
    • B06B1/0223Driving circuits for generating signals continuous in time
    • B06B1/0238Driving circuits for generating signals continuous in time of a single frequency, e.g. a sine-wave
    • B06B1/0246Driving circuits for generating signals continuous in time of a single frequency, e.g. a sine-wave with a feedback signal
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
    • B01F11/00Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F11/02Mixing by means of high-frequency, e.g. ultrasonic vibrations, e.g. jets impinging against a vibrating plate
    • B01F11/0283Mixing by means of high-frequency, e.g. ultrasonic vibrations, e.g. jets impinging against a vibrating plate transmitting the vibratory energy by means of a fluid, e.g. by means of air shock waves
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
    • B01F11/00Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F11/02Mixing by means of high-frequency, e.g. ultrasonic vibrations, e.g. jets impinging against a vibrating plate
    • B01F11/0291Methodical aspects; Controlling
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06Lysis of microorganisms
    • C12N1/066Lysis of microorganisms by physical methods
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves

Abstract

The invention relates to a system (100) and a method (500) for monitoring a vibration transmitting unit (300), in particular a sonotrode (300), for introducing mechanical vibrations into a device (200) for exciting a device (200) Fluid (210), in particular for lysing biological cells in a sample fluid (210), the system (100) having an acoustic sensor (110), in particular a microphone (110), for receiving mechanical vibrations of the device (200), the system (100) further comprising control means (120) coupled to the sensor (110) for analyzing the mechanical vibrations sensed by the sensor (110), the control means (120) being arranged to initiate operation of the vibration transmitting unit (300) ) when the recorded mechanical vibrations have predetermined acoustic characteristics.

Description

  • State of the art
  • The detection of bacterial or cellular pathogens in a sample can be done via the polymerase chain reaction (PCR for short). In this procedure, typical DNA strands are detected for identification of the target cell comprising the DNA. To make this possible, the DNA must be extracted from the cell, in which the cell membrane is broken, which is referred to as lysis. A typical lysis method is ultrasonic lysis, in which ultrasound excitation in the sample fluid generates cavitation bubbles which, on disintegration, produce shear forces that can rupture biological cell membranes. This produces a typical acoustic signal when the cavitation bubbles collapse.
  • From the prior art, the use of a high-frequency oscillating excitation head is known, also called sonotrode, wherein the sonotrode is immersed directly in the liquid to be lysed. Other methods, such as in US20140120599 A1 and GB2403729 On the other hand, it is possible to transmit the excitation from the sonotrode via an elastic membrane, for example a polymer membrane, which can be referred to as indirect ultrasound treatment. This has the advantage that the sample volume can be closed and the sonotrode can remain contamination-free.
  • When using ultrasound lysis, care must be taken not to "dry" the membrane, which means that the membrane is at least partially out of contact with the sample fluid. Such draining can lead to rapid heating and eventual destruction of the membrane. For example, a localized draining by boiling and thus a formation of bubbles of the sample liquid take place. Other causes of drainage may be an insufficiently filled sample chamber or an unintentional loss of sample fluid.
  • Disclosure of the invention
  • Advantages of the invention
  • Against this background, the invention relates to a system for monitoring a vibration transmitting unit. The vibration-transmitting unit can be used to initiate mechanical vibrations in a device for exciting a fluid in the device, in particular for introducing mechanical vibrations in a lysis device for lysis of biological cells in a sample liquid. The system has an acoustic sensor for receiving mechanical vibrations, in particular vibrations of the device. Furthermore, the system comprises a control device connected to the acoustic sensor for analyzing the mechanical vibrations recorded by the sensor. The control device is set up to reduce an operation of the vibration-transmitting unit when the recorded mechanical vibrations have predetermined acoustic characteristics.
  • A vibration-transmitting unit may in particular be understood as a sonotrode, for example an ultrasound sonotrode. The acoustic sensor may in particular be a microphone, for example a MEMS microphone. The system may include the vibration transmitting unit. An excitation of a liquid may, in particular, be understood to mean a mechanical vibration due to the transmitted mechanical vibrations, for example for a thorough mixing of the liquid. For example, the device can be provided for a mixing of two or more substances, for example for homogenization due to mixing or for an emulsion of suspensions. As stated above, the device can also be a lysis device, ie a device for lysing cells, in particular a microfluidic device such as, for example, a lab-on-a-chip system. A lysis of a cell can be understood to mean a breakdown or destruction of a cell membrane of the cell, for example by mechanical stress as described above. The cells may be located in a sample liquid, for example in a biological sample such as blood, sputum or a smear. The control device may be an electrical circuit, which in particular comprises an evaluation device, for example a processor or microprocessor, and / or a control device. The evaluation device is set up, in particular programmed, to analyze the mechanical vibrations recorded by the sensor for the given acoustic characteristics and, if present, reduce the operation of the vibration-transmitting unit. Specified acoustic features are to be understood in particular as properties of the mechanical vibrations, such as predetermined frequencies, frequency ranges, amplitudes and / or amplitude ranges, as well as predetermined combinations of these quantities. A reduction of the operation is to be understood in particular as a reduction of the vibration power output by the vibration-transmitting unit, in particular one Reduction of one or more amplitudes of transmitted mechanical vibrations.
  • The system according to the invention has the advantage that a quality of an oscillation of the device excited by mechanical vibrations can be acoustically monitored. Furthermore, advantageously, the introduction of mechanical vibrations can be rapidly and easily reduced or even completely interrupted, if a decrease in the quality of the vibrations is detected by detecting the predetermined acoustic characteristics via the system according to the invention. The invention has the particular advantage that a drainage of a standing in contact with the liquid membrane can be detected early by the concomitant change in the mechanical vibrations and thus averted. As stated above, such a drainage can be done, for example, when due to the initiation of the mechanical vibration, the liquid begins to boil. The system according to the invention thus makes it possible to reduce the risk of impairment of integrity or even damage to the device and in particular the membrane by unintentional vibrations. In particular, the risk of otherwise very rapid and intense heating and possibly even damage by partial melting of the device or the membrane with possible leakage of the liquid is thus advantageously reduced.
  • According to a particularly preferred embodiment of the invention, the predetermined features represent properties, in particular amplitudes, of subharmonic or superharmonic suggestions of the mechanical oscillations of the device caused by the vibration-transmitting unit. In an operation of the vibration transmitting unit, the unit and the apparatus form an oscillating overall system. When drainage of the membrane occurs, the overall system corresponds to a weakly damped, non-linear oscillator in which, in addition to a mechanical vibration at an original excitation frequency of the vibration transmitting unit, vibrations at integer multiples of that original excitation frequency (so-called superharmonic excitations) and oscillations at integer parts this original excitation frequency (so-called subharmonic excitations) can occur. Drying of the membrane thus leads in particular to a reduced attenuation of the device brought into oscillation, so that, in particular, subharmonic excitations increasingly occur. Preferably, the predetermined features therefore represent subharmonic vibrations of the device. Advantageously, by determining the superharmonic and / or in particular the subharmonic suggestions via the control device of the system according to the invention, it is therefore possible to detect a drainage of the membrane at an early stage. In other words, the control device is thus arranged to reduce the operation of the vibration-transmitting unit when the recorded mechanical vibrations comprise one or more superharmonic and / or preferably subharmonic excitations in a predetermined strength.
  • In a further advantageous embodiment of the system according to the invention, the predetermined features represent a predetermined change of mechanical vibrations of predetermined frequencies. Specified frequencies may, in particular, be understood as meaning one or more discrete frequencies and / or one or more frequency ranges, which are predetermined. Such a change, in particular an increase of mechanical vibrations of predetermined frequencies, may also indicate a reduced damping of the forced vibration device and thus advantageously allow an early response thereto. In other words, the control device is thus arranged to reduce the operation of the vibration transmitting unit when the recorded mechanical vibrations comprise one or more vibrations of certain frequencies in a predetermined magnitude or with a predetermined change.
  • The control device is preferably set up to reduce or alternatively interrupt the operation of the vibration-transmitting unit if an indicator value derived from a combination of a plurality of predetermined acoustic features or alternatively a change in the derived indicator value exceeds a predetermined threshold value. This has the advantage that a threshold value comparison for a possible reduction of the operation can be carried out from several different sizes. For example, the indicator value may correspond to a total power of a signal received by the acoustic sensor of a predetermined spectrum of vibration frequencies. Alternatively, the indicator value may correspond to a ratio of a first total power of a first spectrum of vibration frequencies to a second total power of a second spectrum of vibration frequencies, wherein the first spectrum is, for example, a partial spectrum of the second spectrum. Alternatively, the indicator value may correspond to a ratio of a signal level of a subharmonic vibration to the signal level of the vibration given by the vibration transmitting unit, where, for example, the frequency of the subharmonic oscillation corresponds to half the frequency of the predetermined oscillation. Alternatively, the indicator value may also correspond to a sum of powers of all subharmonic vibrations up to a predetermined order.
  • According to a particularly advantageous development of the invention, the control device is set up to interrupt or stop the operation of the vibration-transmitting unit when the recorded mechanical vibrations have predetermined acoustic characteristics. This has the advantage that the transmission of the mechanical vibrations can be completely stopped.
  • Preferably, the system is set up to interrupt a power supply of the vibration transmitting unit for the interruption of the operation. For example, the system may have an interface for connection to a power source of the vibration-transmitting unit. The interface may be a digital or analog interface for regulating the power of the vibration transmitting unit and / or the power control of a generator for generating the vibrations, in particular an ultrasonic generator. The interface may include a relay for decoupling the generator from a power source of the generator.
  • In an advantageous development of the invention, the system comprises the device, wherein the device has a vibratable membrane for transmitting mechanical vibrations to the liquid and wherein the acoustic sensor is arranged with respect to the device, in particular the membrane, that the sensor mechanical vibrations of the membrane can record. This has the advantage that the focus of the monitoring is directed to the vibration excitation of the membrane. In particular, an approaching or incipient drainage of the membrane can be recognized particularly early.
  • The invention also provides a method for monitoring a vibration-transmitting unit. In a first step of the method, mechanical vibrations are introduced into a device for exciting a liquid present in the device, in particular for lysing biological cells in a sample liquid, via a vibration-transmitting unit, in particular a sonotrode. In a second step, mechanical vibrations of the device are recorded by an acoustic sensor, wherein the acoustic sensor is connected to a control device of the system, wherein the control device is set up for an analysis of the recorded mechanical vibrations. In a third step, the operation of the vibration transmitting unit by the control device of the system is reduced when the mechanical vibrations have predetermined acoustic characteristics.
  • For the advantages of the method according to the invention, reference is also made to the above-mentioned corresponding advantages of the device according to the invention.
  • list of figures
  • Embodiments of the invention are shown schematically in the drawings and explained in more detail in the following description.
  • Show it
    • 1 an embodiment of the system according to the invention and
    • 2 a flowchart for an embodiment of the method according to the invention.
  • Embodiments of the invention
  • 1 schematically shows an embodiment of the system according to the invention 100 , The system 100 is next to a device 200 arranged, the device 200 a container 220 with a liquid in it 210 includes. The container 220 with an exemplary capacity between 500 and 1000 microliters is via an elastic membrane 230 with a vibration transmitting unit 300 in contact. The membrane 230 has an exemplary thickness between 100 and 400 microns and consists for example of an elastic polymer. In this example, the vibration transmitting unit 300 to an ultrasonic sonotrode 300 with an excitation frequency between 20 and 40 kilohertz and a sonotrode zero excursion of the sonotrode head between 2 and 20 microns, which via the elastic membrane 230 mechanical vibrations on the liquid 210 in the container transfers. In this example, the fluid is a biological sample, such as blood or sputum, which comprises cells to be lysed. Thus, the device serves 200 together with the sonotrode 300 an exemplary ultrasonic lysis of the cells to subsequently detect DNA released from the cells for identification of the cells.
  • The system 100 includes an acoustic sensor 110 , in this example, a microphone 110 , With the microphone 110 For example, it can be a space-efficient MEMS microphone. Preferably, the microphone has 110 a higher sampling rate than the excitation frequency of the sonotrode 300 . For example, a sample rate that is greater than twice the excitation frequency of the sonotrode 300 is. The microphone 110 is so with respect to the device 200 is arranged that there are mechanical vibrations of the device 200 can record continuously. To drain the membrane 230 The microphone can be detected early 110 as close to the membrane as possible 230 to be ordered. The system 100 further includes one with the microphone 110 connected control device 120 as an evaluation device for an analysis of the microphone 110 absorbed mechanical vibrations of the membrane 230 , The control device 120 includes a microprocessor 125 and a controller 126 for controlling a reduction or deactivation of the operation of the sonotrode 300 , The control unit 126 can the microprocessor 125 or others for controlling the microphone 110 and / or the sonotrode 300 equipped microprocessors include. This can be done by the system 100 with a control device 310 and / or an energy source 320 the sonotrode 300 be connected, in particular via an interface 140 In an alternative embodiment, the system includes 100 further the sonotrode 300 and / or the device 200 ,
  • The control device 120 is set up, in particular via a programming of the microprocessor 125 and the controller 126 , the operation of the sonotrode 300 reduce if the recorded mechanical vibrations have predetermined acoustic characteristics. The given features may in particular be amplitudes of subharmonic vibrations of the device 200 concerning by sonotrode 300 act transferred fundamental. Preferably, the control device 120 set up the operation of the sonotrode 300 restrict or interrupt when an indicator value derived from a combination of a plurality of predetermined acoustic features or, alternatively, a change in the derived indicator value exceeds a predetermined threshold, the indicator value corresponding to the above examples
  • 2 shows a flowchart for an embodiment of the method according to the invention 500 , which for example with the zu 1 described embodiment of the system according to the invention 100 can be executed.
  • In a first step 501 of the procedure 500 an introduction of mechanical vibrations takes place in the device 200 for lysing the biological cells in the sample fluid 210 over the sonotrode 300 , In a second step 502 become mechanical vibrations of the membrane 230 the device 200 through the microphone 110 added. In a third step 503 there is a reduction in the operation of the sonotrode 300 by the control device 310 of the system 100 when the mechanical vibrations have predetermined acoustic characteristics, as described above.
  • QUOTES INCLUDE IN THE DESCRIPTION
  • This list of the documents listed by the applicant has been generated automatically and is included solely for the better information of the reader. The list is not part of the German patent or utility model application. The DPMA assumes no liability for any errors or omissions.
  • Cited patent literature
    • US 20140120599 A1 [0002]
    • GB 2403729 [0002]

Claims (10)

  1. System (100) for monitoring a vibration-transmitting unit (300), in particular a sonotrode (300), for introducing mechanical vibrations into a device (200) for exciting a liquid (210) in the device (200), in particular for lysis of biological cells in a sample liquid (210), the system (100) comprising an acoustic sensor (110), in particular a microphone (110) for receiving mechanical vibrations of the device (200), the system (100) further having a Sensor (110) connected to control means (120) for analyzing the mechanical vibrations received by the sensor (110), characterized in that the control means (120) is arranged to reduce operation of the vibration transmitting unit (300) when picked up mechanical vibrations have predetermined acoustic characteristics.
  2. System (100) after Claim 1 wherein the predetermined acoustic characteristics represent properties, in particular amplitudes, of subharmonic or superharmonic excitations of the mechanical vibrations of the device (200) caused by the vibration transmitting unit (300).
  3. The system (100) of any one of the preceding claims, wherein the predetermined acoustic features represent a predetermined change of vibrations of predetermined frequencies.
  4. System (100) after Claim 3 wherein the predetermined change comprises an increase of mechanical vibrations of predetermined frequencies.
  5. The system (100) of any of the preceding claims, wherein the controller (120) is arranged to reduce or alternatively interrupt the operation of the vibration transmitting unit (300) when an indicator value derived from a combination of a plurality of predetermined acoustic features or alternatively a change of the derived indicator value exceeds a predetermined threshold.
  6. System (100) after Claim 5 wherein the indicator value corresponds to a ratio of a first total power of a first spectrum of vibration frequencies to a second total power of a second spectrum of vibration frequencies, wherein the first spectrum is preferably a partial spectrum of the second spectrum.
  7. The system (100) of any one of the preceding claims, wherein the control means (120) is arranged to interrupt or stop the operation of the vibration transmitting unit (300) when the received mechanical vibrations have predetermined acoustic characteristics.
  8. System (100) after Claim 7 wherein the system (100) is arranged to interrupt a power supply (320) of the vibration transmitting unit (300) for the interruption of the operation.
  9. The system (100) of any one of the preceding claims, wherein the system (100) comprises the apparatus (200), the apparatus (200) comprising a vibratable membrane (230) for transmitting mechanical vibrations to the liquid (210), and wherein the acoustic Sensor (110) is arranged with respect to the device (200) that the sensor (110) can absorb mechanical vibrations of the membrane (230).
  10. A method (500) for monitoring a vibration transmitting unit (300), comprising the steps of: Introduction of mechanical oscillations into a device (200) for exciting a liquid (210) in the device, in particular for lysing biological cells in a sample liquid (210), via a vibration-transmitting unit (300), in particular a sonotrode (300) , • Receiving mechanical vibrations of the device (200) by an acoustic sensor (110), the acoustic sensor (110) being connected to a control device (120) of the system, the control device (120) being arranged for analysis of the recorded mechanical vibrations is. • Reduction of operation of the vibration transmitting unit (300) by the controller (120) of the system (100) when the mechanical vibrations have predetermined acoustic characteristics.
DE102017219177.5A 2017-10-26 2017-10-26 System and method for monitoring a vibration transmitting unit, in particular a sonotrode for ultrasonic lysis Pending DE102017219177A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE102017219177.5A DE102017219177A1 (en) 2017-10-26 2017-10-26 System and method for monitoring a vibration transmitting unit, in particular a sonotrode for ultrasonic lysis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102017219177.5A DE102017219177A1 (en) 2017-10-26 2017-10-26 System and method for monitoring a vibration transmitting unit, in particular a sonotrode for ultrasonic lysis
PCT/EP2018/078146 WO2019081260A1 (en) 2017-10-26 2018-10-16 System and method for monitoring a vibration-transmitting unit, in particular a sonotrode for ultrasonic lysis

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DE102017219177A1 true DE102017219177A1 (en) 2019-05-02

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WO (1) WO2019081260A1 (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000053263A1 (en) * 1999-03-08 2000-09-14 Angiosonics Inc. Dual transducer ultrasound lysis method and apparatus
GB2403729A (en) 2003-07-11 2005-01-12 Qinetiq Ltd Sonicator device and method
US20140120599A1 (en) 2012-10-25 2014-05-01 Robert Bosch Gmbh Devices, method and system employable for lysing
EP3184624A1 (en) * 2015-12-22 2017-06-28 Diagenode S.A. Frequency optimized devices and methods for microfluidic sonication
EP3210540A1 (en) * 2009-03-20 2017-08-30 University of Cincinnati Ultrasound-mediated inducement, detection, and enhancement of stable cavitation

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6291180B1 (en) * 1999-09-29 2001-09-18 American Registry Of Pathology Ultrasound-mediated high-speed biological reaction and tissue processing
US8459121B2 (en) * 2010-10-28 2013-06-11 Covaris, Inc. Method and system for acoustically treating material

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000053263A1 (en) * 1999-03-08 2000-09-14 Angiosonics Inc. Dual transducer ultrasound lysis method and apparatus
GB2403729A (en) 2003-07-11 2005-01-12 Qinetiq Ltd Sonicator device and method
EP3210540A1 (en) * 2009-03-20 2017-08-30 University of Cincinnati Ultrasound-mediated inducement, detection, and enhancement of stable cavitation
US20140120599A1 (en) 2012-10-25 2014-05-01 Robert Bosch Gmbh Devices, method and system employable for lysing
EP3184624A1 (en) * 2015-12-22 2017-06-28 Diagenode S.A. Frequency optimized devices and methods for microfluidic sonication

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