DE102016210357A1 - Method and device for detecting an occupation of a surface by means of induced fluorescence - Google Patents

Method and device for detecting an occupation of a surface by means of induced fluorescence

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Publication number
DE102016210357A1
DE102016210357A1 DE102016210357.1A DE102016210357A DE102016210357A1 DE 102016210357 A1 DE102016210357 A1 DE 102016210357A1 DE 102016210357 A DE102016210357 A DE 102016210357A DE 102016210357 A1 DE102016210357 A1 DE 102016210357A1
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Prior art keywords
fluorescence
excitation
surface
radiation
light
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Withdrawn
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DE102016210357.1A
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German (de)
Inventor
Hauke Brüning
Kai Brune
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Fraunhofer Gesellschaft zur Forderung der Angewandten Forschung eV
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Fraunhofer Gesellschaft zur Forderung der Angewandten Forschung eV
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Priority to DE102016210357.1A priority Critical patent/DE102016210357A1/en
Publication of DE102016210357A1 publication Critical patent/DE102016210357A1/en
Application status is Withdrawn legal-status Critical

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6402Atomic fluorescence; Laser induced fluorescence
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N2021/6417Spectrofluorimetric devices
    • G01N2021/6419Excitation at two or more wavelengths
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N2021/6417Spectrofluorimetric devices
    • G01N2021/6421Measuring at two or more wavelengths
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters
    • G01N21/6456Spatial resolved fluorescence measurements; Imaging
    • G01N2021/646Detecting fluorescent inhomogeneities at a position, e.g. for detecting defects

Abstract

The present invention relates to the field of detecting an occupation of a surface (ie a check whether and to what extent on or on a surface, for example, adheres or rests unwanted material) and proposes a method and an apparatus for detecting an occupancy of a surface means induced fluorescence, wherein the surface is exposed to induce fluorescence at least in the surface of an excitation and excitation-induced fluorescence radiation is measured. For inducing the fluorescence, a first excitation and a second excitation, which differs from the first excitation, are used and / or the fluorescence radiation is measured separately in a first and a second radiation wavelength range, wherein the presence of an occupancy by comparison is at least a measurement result is determined with a reference.

Description

  • The present invention relates to the field of detecting an occupation of a surface (ie a check whether and to what extent on or on a surface, for example, unwanted material adheres or rests) and in particular the detection of the occupancy of the surface by means of induced fluorescence.
  • Laser-induced fluorescence (LIF) is a widely used method for detecting contamination of surfaces, especially on metallic substrates with oils or the like. Here, not only qualitative measurements but also quantitative measurements of the occupation quantity can be carried out after calibration. On surfaces which themselves fluoresce strongly, e.g. Carbon fiber reinforced plastics (CFRP) or other plastic surfaces, the technique used in metal surfaces generally can not be used because the fluorescence of the substrate superimposes the often weaker fluorescence of the contamination.
  • In the field of contamination detection on metallic surfaces, there are already many commercially available systems that are used in many areas. In many cases, these are very simple, robust measurement structures that can do with few components and can also be used as handheld devices. In most cases, only a single LED and a photodiode are needed. For this reason, this technique is widely used in many industries and is e.g. used in the automotive industry in the incoming goods inspection. However, these devices are not suitable for use on plastic surfaces due to the above problem.
  • In order to allow measurements on such and other self-fluorescent surfaces, a higher equipment and metrological effort must be operated. For this purpose, pulsed laser systems are often used as the excitation source and then the fluorescence is examined time-dependent. Such a procedure, as for example in DE 195 07 119 A1 is described in addition to the required components much higher demands on occupational safety, since laser protection classes must be observed. Also, the obtained measurement data must be elaborately evaluated.
  • Another possibility is to take up the complete fluorescence spectrum and then evaluate it, as described e.g. in medical technology in the field of cancer diagnostics. However, in this case the required effort is once again higher, in addition to a more complex detector, VIS spectrometer, fall even larger amounts of measurement data to be evaluated.
  • An object underlying the present invention is to provide a method and a device for detecting a surface occupation by means of induced fluorescence, since the disadvantages present in the prior art are avoided or at least reduced, that is to say in particular with a simple apparatus design, few restrictions and low Data evaluation requirements allow detection of occupancy by induced fluorescence on even fluorescence-excited surfaces.
  • According to the invention, according to a first aspect, a method for detecting an occupancy of a surface by means of induced fluorescence is proposed, as defined in claim 1, namely a method in which the surface is exposed to induce fluorescence at least in the surface of an excitation and one by the Excitation induced fluorescence radiation is measured, wherein for inducing the fluorescence, a first excitation and a second excitation, which differs from the first excitation, are used and / or the fluorescence radiation is measured separately in a first and a second radiation wavelength range wherein the presence of an occupancy is determined by comparing at least one measurement result with a reference.
  • According to a second aspect of the invention, a device for detecting an occupancy of a surface by means of induced fluorescence is proposed, as defined in claim 12, in which the device is designed to expose the surface for inducing fluorescence at least in the surface of an excitation and excitation-induced fluorescence radiation, the device comprising an excitation unit for exposing the surface of a first excitation to induce fluorescence and a second excitation for inducing fluorescence different from the first excitation, and / or the device first measuring unit for measuring the fluorescence radiation in a first radiation wavelength range and a second measuring unit for measuring the fluorescence radiation in a second radiation wavelength range, wherein the device comprises a determination unit for determining the presence of ens an occupancy by comparing at least one measurement result with a reference.
  • In order to enable a measurement of contaminations, in particular on even highly fluorescent materials, the invention provides, on the one hand, that the resulting Stimulating the fluorescence spectrum at two different wavelengths so as to exploit the different excitation characteristics of the substrate and contaminant material, and (complementary or alternative) to measure the resulting spectrum at at least two different sites and then exploiting the different emission characteristics. In both cases, the invention allows a deviation from the pure fluorescence of the substrate material (ie the surface) to be detected.
  • A possible implementation of the invention provides that for excitation two light sources, preferably LEDs, are used with wavelengths in different spectral ranges. Since in most cases the excitation spectra of substrate material and the overlaying contamination are different, a measurable change due to contamination is expected.
  • Another implementation of the invention, as is common in most devices available on the market, is to use only a single light source for excitation. It is used that the emission spectra of substrate and contamination at excitation even with only one wavelength have differences, and two or more detectors are used for the measurement. These detectors are sensitive to fluorescence in different spectral ranges for the resulting light and thus carry out a partial measurement of the resulting spectrum at several points.
  • The exact interpretation of an implementation of the present invention (possibly also by combining different aspects) depends on the particular application, in which case various technical issues may also play a role. Thus, narrow-band filters for detectors are more cost-effective to produce than narrow-band LEDs, but it may be easier to distinguish the spectra with different excitations.
  • An advantage of the present invention is the comparatively simple technical implementation and use. Like the devices already in the market for metal surfaces, the invention can be implemented using simple, robust and inexpensive components. This has significant advantages in a commercial use. At the same time, the invention allows a very fast measurement and evaluation, since no complex interpretation of the spectra or the time response is necessary. This makes it possible to measure larger areas in a short time.
  • Basically, the present invention can be used in all areas in which LIF is already used on metal surfaces and where plastic surfaces such as CFRP are to be increasingly used in the future. This includes, for example, the incoming goods inspection in the automotive industry or quality assurance in the field of adhesive technology. Especially in the course of the introduction of DIN 2304 A QA concept and continuous monitoring of the bonding processes is becoming increasingly important. In this case, a simple method such as that of the present invention, which can also be implemented very well in a hand-held device, has excellent application possibilities.
  • In an advantageous embodiment of an aspect of the invention, the excitation for inducing fluorescence comprises irradiating light suitable for inducing fluorescence at least in the surface, and the first excitation comprises irradiating the surface with light in a first light wavelength range and the second excitation comprises irradiation of the surface with light in a second light wavelength range. Although the invention is not limited to the use of light to induce fluorescence, the use of light provides a preferred embodiment. As an alternative or in addition to the use of light as excitation, other types of radiation, chemical effects, electrical and / or magnetic fields, sound waves or other pressure effects can be used to excite the fluorescence. The person skilled in (induced) fluorescence is able to transform the examples given below, in which light is used, appropriately to other ways of inducing fluorescence.
  • In a preferred variant of the above embodiment, the first light wavelength range is within the range of 200 to 800 nm, preferably within the range of 350 to 450 nm, and the second light wavelength range is within the range of 350 to 800 nm, preferably within of the range of 350 to 450 nm. These areas, in each of which preferably the first and second light wavelength range falls, can be realized by simply and cheaply available elements (eg light emitting diodes, possibly with a bandpass filter).
  • In a further preferred variant of the above embodiment, light is a laser light or a light emitted by a light emitting diode. The light wavelength range used for the excitation can certainly also be very narrow-band, so that in extreme cases the respective light wavelength range can be limited to a single wavelength.
  • In another advantageous embodiment of an aspect of the invention, the first excitation and / or the second excitation are suitable for inducing fluorescence in the occupancy. Fluorescence is also preferably induced in the occupancy (s) by at least one of the excitations, although the present invention also allows attenuation or suppression of the incoming excitation (eg of the incident light) and / or outgoing fluorescence radiation can be measured by occupancy. It is also possible to use a displacement of approximately the wavelength (s) of irradiated light and / or a displacement of the wavelengths of the fluorescence radiation emerging from the surface through the occupancy for detection.
  • In another advantageous embodiment of an aspect of the invention, the first and the second excitation take place at least partially simultaneously, wherein the reference has an intensity of the fluorescence radiation. Given a suitable choice of the first and second excitation, depending on the presence or absence of an occupancy, a fluorescence radiation (in the spectrum as a whole and in particular in intensity) results, so that their measurement permits a corresponding detection.
  • In another advantageous embodiment of an aspect of the invention, the first and the second excitation take place at least partially separated in time, wherein the reference has a respective intensity of the fluorescence radiation and / or a ratio of the intensities of the fluorescence radiation. In the case of an excitation which is at least partially separated in time, the fluorescence radiation induced in each case by the different excitations can also be measured separately in time so that, in particular, a comparison of intensities for detecting the occupation is possible.
  • In another advantageous embodiment of an aspect of the invention, the fluorescence radiation is measured separately in a first and a second radiation waveband and the reference has a respective intensity of the fluorescence radiation in the first and the second radiation waveband and / or a ratio the intensities of the fluorescence radiation in the first and the second radiation waveband. In general, or at least with a suitable choice of the radiation wavelength ranges, the induced fluorescence (spectra) of the surface and the occupancy differ, so that in this case again the presence or absence of an occupancy can be inferred.
  • In another advantageous embodiment of an aspect of the invention, in addition to the first and second excitations, a third excitation, which differs from the first and the second excitation, is used to induce the fluorescence. If further suggestions are used, additional possibilities of differentiation result, so that, for example, a distinction can also be made, at least qualitatively, between different assignments.
  • In another advantageous embodiment of an aspect of the invention, the measurement of the fluorescence radiation is carried out separately in addition to the first and second radiation wavelength range in a third radiation wavelength range. With the use of further radiation wavelength ranges, additional possibilities of differentiation result, so that, for example, at least qualitatively a distinction can be made between different assignments.
  • In another advantageous embodiment of an aspect of the invention, a determination of the reference is furthermore carried out, the determination of the reference being a measurement on a known occupied or unoccupied reference surface, a determination of a measurement basis of measurements on the surface and / or a calculation based on Information on the surface and / or possible assignments includes. One way to determine a reference is to make a comparative measurement. Here, "known occupied or unoccupied reference surface" means that at least one relevant property (e.g., occupancy level) of the reference surface is basically known, and it is sufficient that the occupancy level is merely known to be acceptable. In this context, it can also be (only) determined that the current surface (where the current measurement is made) is "better" than another (reference) surface. Within the framework of a measurement passage over a certain surface area, a predominantly present measured value can be used as a reference, for example if it may be assumed that the surface in question is predominantly unoccupied and has only occasional occupancies. The same applies to the opposite case, if it can be assumed that there is predominantly a (for example, desired) occupancy, which is interrupted only in smaller parts. Defined values for surfaces can be specified which make a measurement of the reference unnecessary, wherein such a value specification can also be carried out, in particular without reference measurements, on only theoretically determined values (values calculated from fundamental considerations, for example).
  • In another advantageous embodiment of an aspect of the invention, the excitation for inducing fluorescence comprises irradiation with Light suitable for inducing fluorescence at least in the surface and the excitation unit comprises a first light source for irradiating the surface with light suitable for inducing fluorescence at least in the surface, in a first light wavelength range, and a second light source for irradiating the surface with light suitable for inducing fluorescence at least in the surface in a second light wavelength range. In general, a structure in which different light sources are used for different wavelength ranges is easier, especially if the different wavelength ranges are to be provided at different times.
  • In another advantageous embodiment of an aspect of the invention, a first filter for supplying fluorescence radiation in the first radiation wavelength range to a first detector and a second filter for supplying fluorescence radiation in the second radiation wavelength range to a second detector are provided , If the radiation wavelength ranges are separated by filters, the respective detector does not require a separate resolution with regard to the wavelengths (or the whole of the entire spectrum) of the incident radiation, and an intensity determination summing over the wavelength range is sufficient.
  • According to the invention, a computer program with program means is proposed according to a further aspect, which cause a device according to claim 12 to carry out the steps of the method according to the invention as claimed in claim 1, when the computer program is executed on the device. The computer program may be provided, stored and / or distributed on a suitable storage medium, such as an optical storage medium or a non-volatile electronic storage medium. It can also be provided together with or as part of a hardware component. The computer program may also be provided in other ways, such as via the Internet or via wired or wireless telecommunications means.
  • Features of advantageous embodiments of the invention are defined in particular in the dependent claims, wherein further advantageous features, embodiments and embodiments for the skilled person also from the above explanation and the following discussion can be found.
  • In the following, the present invention is further illustrated and explained with reference to exemplary embodiments illustrated in the figures. This shows
  • 1 1 is a schematic illustration for illustrating a first exemplary embodiment of a device according to the invention,
  • 2 FIG. 2 a schematic illustration for illustrating a second exemplary embodiment of a device according to the invention, FIG.
  • 3 a schematic flow diagram of a first embodiment of a method according to the invention and
  • 4 a schematic flow diagram of a second embodiment of a method according to the invention.
  • In the accompanying drawings, as well as the explanations to these drawings, corresponding or related elements, as appropriate, are identified with corresponding or corresponding reference numerals, even though they may be found in different embodiments.
  • 1 shows a schematic representation for illustrating a first embodiment of a device according to the invention.
  • The device is used to detect an occupancy of a substrate or its surface 1 with a contamination (not shown), with both the surface 1 as well as the contamination can be excited to fluorescence.
  • The device comprises an excitation unit 2 with a first laser source 7 with a first wavelength and a second laser source 8th with a second wavelength different from the first wavelength. The respective editions of the laser sources 7 . 8th be through a beam combiner 9 the excitation unit 2 as a ray 4 on the surface 1 directed, where by the irradiation of the laser light with the at least two excitation wavelengths in each case a fluorescence in the surface 1 and the contamination is induced.
  • The resulting fluorescence radiation 5 is fitted with a suitable deflection unit (filter 6 ) to a detector 3 as an example of a measuring unit, so that the intensity of the fluorescence can be determined.
  • The irradiation with the different wavelengths and the corresponding induction of the fluorescence takes place at different times, so that the detection of the fluorescence radiation likewise takes place correspondingly at different times. However, this does not focus on the time behavior of a fluorescence radiation stimulated in each case (for example by a pulse), since here approximately at a continuous irradiation with, for example, the first wavelength, the resulting fluorescence radiation is measured, which is stable after a very short transient period.
  • From the different intensities at different excitation wavelengths, resulting from the different characteristics of surface fluorescence and contamination, it is possible to deduce the presence or absence of contamination. With appropriate calibration, a quantitative determination is possible.
  • 2 shows a schematic representation for illustrating a second embodiment of a device according to the invention.
  • As in the case of in 1 Illustrated embodiment is also in the case of 2 the surface 1 , whose occupancy is to be detected by a contamination, from an excitation unit 2 ' irradiated with light that induces fluorescence. Unlike in the case of 1 includes the excitation unit 2 ' only one (laser) light source, so the excitation or the light beam 4 is limited to a light wavelength range or a wavelength.
  • The fluorescence radiation induced in the surface and possibly the contamination thereon 5 is through suitable, wavelength-specific filters 6 ' . 6 '' on two detectors (measuring units) 3 ' . 3 '' steered so that the detectors 3 ' . 3 '' in each case one intensity of the fluorescence radiation can detect a first radiation wavelength range and a second radiation wavelength range separately from one another.
  • For the two in 1 and 2 illustrated embodiments holds that a determination unit (not shown separately) determined from the measurement results at least the presence of the occupancy by the contamination.
  • The use of two different excitations and / or the observation of two radiation wavelength ranges allows the influence of non-occupancy factors on the intensity of the fluorescence radiation (eg distance between detector and surface, fundamental surface structures or textures, directional dependencies, etc .), The realization is used that the fluorescence spectra of surface (or substrate) and occupancy (such as a contamination) are different.
  • In the case of an unoccupied surface, a respective intensity of a resulting fluorescence radiation is at different excitations in a ratio determined, for example, by a reference measurement. The contribution by an occupancy to the fluorescence radiation is also in a certain ratio, but this is different than the ratio of the surface, so that the resulting total fluorescence radiation of the combination of surface and occupancy at different excitations each have intensities whose ratio detected deviates from that in the case of a blank surface. It is also possible to detect other influences of the occupancy on the fluorescence radiation (direct influences or indirect influences by interaction between the excitation and the occupancy before the fluorescence is induced), so that it is possible to conclude the presence of an occupancy.
  • 3 shows a schematic flow diagram of a first embodiment of a method according to the invention.
  • In step 10 a first excitation takes place in the form of an irradiation of the surface to be tested with fluorescence-inducing light. The surface (and possibly the assignment) is excited by the irradiation to fluorescence (step 11 ), where the fluorescence radiation in step 12 is detected.
  • In step 13 a second excitation takes place in the form of an irradiation of the surface to be tested with fluorescence-inducing light, which, however, has a different wavelength than that in step 10 has related light. In the steps 14 and 15 There is also a transmission of fluorescence radiation and a corresponding measurement.
  • The steps 10 to 12 on the one hand and 13 to 15 on the other hand, are separated in time, so that the measurements in the steps 12 and 15 each alone to the suggestions in the steps 10 and 13 appropriate intensities of the respective fluorescence radiation can determine.
  • From processing the measurement results in step 16 determines whether or not there is an occupancy.
  • 4 shows a schematic flow diagram of a second embodiment of a method according to the invention.
  • In step 20 an excitation takes place in the form of an irradiation of the surface to be tested with fluorescence-inducing light. This irradiation leads to fluorescence radiation (eg, only the surface at a blank surface and from the surface and occupancy together with an occupied surface) (step 21 ).
  • The fluorescence radiation is measured separately in at least two radiation wavelengths (steps 22 and 23 ), again - similar to in 3 illustrated embodiment - from processing the measurement results in step 24 at least the information is obtained whether an occupancy exists or not.
  • Although various aspects or features of the invention are shown in combination in the figures, it will be apparent to those skilled in the art, unless stated otherwise, that the combinations illustrated and discussed are not the only ones possible. In particular, corresponding units or feature complexes from different embodiments can be exchanged with one another.
  • In implementations of the invention, individual components, e.g. a processor, wholly or partly take over the functions of various elements mentioned in the claims. Processes or processes such as, for example, control of elements, acquisition of measured values and their further processing can be implemented as program means of a computer program and / or as special hardware components.
  • QUOTES INCLUDE IN THE DESCRIPTION
  • This list of the documents listed by the applicant has been generated automatically and is included solely for the better information of the reader. The list is not part of the German patent or utility model application. The DPMA assumes no liability for any errors or omissions.
  • Cited patent literature
    • DE 19507119 A1 [0004]
  • Cited non-patent literature
    • DIN 2304 [0014]

Claims (15)

  1. Method for detecting a surface occupied by induced fluorescence, wherein the surface for inducing fluorescence is exposed to excitation at least in the surface and a fluorescence radiation induced by the excitation is measured, characterized in that for inducing the fluorescence, a first excitation and a second excitation, which differs from the first excitation, are used and / or the fluorescence radiation is measured separately in a first and a second radiation wavelength range, wherein the presence of an occupancy is determined by comparing at least one measurement result with a reference.
  2. Method according to claim 1, wherein the excitation for inducing fluorescence comprises irradiating with light suitable for inducing fluorescence at least in the surface, and the first excitation comprises irradiating the surface with light in a first light wavelength range and the second excitation comprises irradiating the surface with light in a second light wavelength range.
  3. The method of claim 2, wherein the first light wavelength range is within the range of 200 to 800 nm, preferably within the range of 350 to 450 nm, and the second light wavelength range is within the range of 350 to 800 nm, preferably within the range from 350 to 450 nm.
  4. The method of claim 2 or 3, wherein the light is a laser light or a light emitted by a light emitting diode.
  5. Method according to one of the preceding claims, wherein the first excitation and / or the second excitation are suitable for inducing a fluorescence in the occupancy.
  6.  Method according to one of the preceding claims, wherein the first and the second excitation at least partially occur at the same time, wherein the reference has an intensity of the fluorescence radiation.
  7. Method according to one of claims 1 to 5, wherein the first and the second excitation occur at least partially separated in time, wherein the reference has a respective intensity of the fluorescence radiation and / or a ratio of the intensities of the fluorescence radiation.
  8. The method of any one of the preceding claims, wherein the fluorescence radiation is measured separately in a first and a second radiation waveband and the reference is a respective intensity of the fluorescence radiation in the first and second radiation waveband and / or a ratio of the intensities having fluorescence radiation in the first and second radiation wavebands.
  9. Method according to one of the preceding claims, wherein for inducing the fluorescence in addition to the first and second excitation, a third excitation is used, which differs from the first and the second excitation.
  10. The method of any one of the preceding claims, wherein the measuring of the fluorescence radiation in addition to the first and second radiation wavelength ranges is performed separately in a third radiation wavelength range.
  11. The method of any one of the preceding claims, further comprising determining the reference, wherein the determination of the reference A measurement on a known occupied or unoccupied reference surface, A determination of a measurement basis from measurements on the surface and / or - includes a calculation based on surface information and / or possible assignments.
  12. Device for detecting an occupation of a surface by means of induced fluorescence, the device being configured to expose the surface for inducing fluorescence at least in the surface of an excitation and to measure a fluorescence radiation induced by the excitation, characterized in that the device comprises and / or comprises an excitation unit for exposing the surface of a first excitation to induce fluorescence and a second excitation for inducing fluorescence different from the first excitation the device comprises a first measuring unit for measuring the fluorescence radiation in a first radiation wavelength range and a second measuring unit for measuring the fluorescence radiation in a second radiation wavelength range, wherein the device comprises a determination unit for determining the presence of an occupancy by comparing at least one measurement result with a reference.
  13. The device of claim 12, wherein the excitation for inducing fluorescence comprises irradiating with light to induce fluorescence at least in the surface, and the excitation unit comprises a first light source for irradiating the surface with light suitable for inducing fluorescence at least in the surface in a first light wavelength range and a second light source for irradiating the surface with light, which is suitable for inducing fluorescence at least in the surface, in a second light wavelength range.
  14. Apparatus according to claim 12 or 13, comprising a first filter for supplying fluorescence radiation in the first radiation wavelength range to a first detector and a second filter for supplying fluorescence radiation in the second radiation wavelength range to a second detector.
  15. A computer program comprising program means for causing an apparatus according to claim 12 to perform the steps of the method of claim 1 when the computer program is executed on the apparatus.
DE102016210357.1A 2016-06-10 2016-06-10 Method and device for detecting an occupation of a surface by means of induced fluorescence Withdrawn DE102016210357A1 (en)

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