DE102006021984B4 - bioreactor - Google Patents

bioreactor

Info

Publication number
DE102006021984B4
DE102006021984B4 DE200610021984 DE102006021984A DE102006021984B4 DE 102006021984 B4 DE102006021984 B4 DE 102006021984B4 DE 200610021984 DE200610021984 DE 200610021984 DE 102006021984 A DE102006021984 A DE 102006021984A DE 102006021984 B4 DE102006021984 B4 DE 102006021984B4
Authority
DE
Germany
Prior art keywords
mixer
characterized
shaft
8th
bioreactor according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE200610021984
Other languages
German (de)
Other versions
DE102006021984A1 (en
Inventor
Dr. Reif Oscar-Werner
Dr. Greller Gerhard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SARTORIUS STEDIM BIOTECH GMBH, 37079 GOETTINGE, DE
Original Assignee
Sartorius Stedim Biotech GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sartorius Stedim Biotech GmbH filed Critical Sartorius Stedim Biotech GmbH
Priority to DE200610021984 priority Critical patent/DE102006021984B4/en
Publication of DE102006021984A1 publication Critical patent/DE102006021984A1/en
Application granted granted Critical
Publication of DE102006021984B4 publication Critical patent/DE102006021984B4/en
Application status is Expired - Fee Related legal-status Critical
Anticipated expiration legal-status Critical

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
    • B01F11/00Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F11/008Mixers with shaking, oscillating, or vibrating mechanisms the stirrers performing an oscillatory, vibratory or shaking movement
    • B01F11/0082Mixers with shaking, oscillating, or vibrating mechanisms the stirrers performing an oscillatory, vibratory or shaking movement performing a rectilinear reciprocating movement
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
    • B01F15/00Accessories for mixers ; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F15/00824Receptacles
    • B01F15/00831Nature of the receptacle
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
    • B01F15/00Accessories for mixers ; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F15/00824Receptacles
    • B01F15/00831Nature of the receptacle
    • B01F15/0085Nature of the receptacle the mixing receptacle being flexible, e.g. flexible bags supported by rigid containers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
    • B01F15/00Accessories for mixers ; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F15/00915Baffles; Flow breakers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
    • B01F7/00Mixers with rotary stirring devices in fixed receptacles, i.e. movement of the receptacle not being meant to effect the mixing; Kneaders
    • B01F7/00658Configuration of the rotating axis
    • B01F7/00683Construction of the axis
    • B01F7/00691The axis being composed of interconnected parts
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
    • B01F7/00Mixers with rotary stirring devices in fixed receptacles, i.e. movement of the receptacle not being meant to effect the mixing; Kneaders
    • B01F7/00658Configuration of the rotating axis
    • B01F7/00683Construction of the axis
    • B01F7/00716Construction of the axis the axis being adjustable in length, e.g. telescopic
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
    • B01F7/00Mixers with rotary stirring devices in fixed receptacles, i.e. movement of the receptacle not being meant to effect the mixing; Kneaders
    • B01F7/16Mixers with rotary stirring devices in fixed receptacles, i.e. movement of the receptacle not being meant to effect the mixing; Kneaders with stirrers rotating about a substantially vertical axis
    • B01F7/18Mixers with rotary stirring devices in fixed receptacles, i.e. movement of the receptacle not being meant to effect the mixing; Kneaders with stirrers rotating about a substantially vertical axis with paddles or arms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/14Bags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/28Constructional details, e.g. recesses, hinges disposable or single use

Abstract

Bioreactor (1), in particular disposable bioreactor with flexible walls, with a reactor interior (2, 2 ', 2' ') delimited by a side wall (5), a bottom part (6) and a ceiling part (7, 7' '), in which at least one mixer (3, 3 ', 3' ') consists of at least one mixing element (8, 8' ') and a mixing shaft (9, 9', 9 '') arranged on the cover part (7, 7 '') , which is driven by a drive arranged outside of the reactor interior (2, 2 ', 2' ') (10), is arranged, characterized in that the mixer (3, 3', 3 '') for variable adaptation to different sizes the reactor interior (2, 2 ', 2' ') is composed of components standardized in size (4) such that the mixer shaft (9, 9', 9 '') consists of a plurality of interlocking shaft elements (11, 13, 13 ''). ) is composable and that the individual shaft elements at (11, 11 '', 13, 13 '') one end of a female connection part (21) and at the opposite end in a female An Closing part (21) insertable male connection part (22).

Description

  • The invention relates to a bioreactor, in particular disposable bioreactor with flexible walls, with a reactor wall bounded by a side wall, a bottom part and a ceiling part, in which at least one mixer consisting of at least one mixing element and a mixing shaft arranged on the ceiling part, from one outside the Reactor interior arranged drive is drivable, is arranged.
  • In particular, bioreactors are increasingly being used as disposable bioreactors with flexible walls of pharmacy and biotechnology. In order to optimize production processes and / or process developments for the cultivation of cells and microorganisms, the production processes and the process development for cultivating cells are first carried out on a scaled-down scale and then optimized later on an enlarged scale in plants with a reactor interior volume of up to 1000 m 3 perform.
  • The disadvantage here is that the so-called scale up in each case must be re-optimized.
  • An already improved bioreactor is out of the WO 03/012027 known. The known bioreactor already has built-in components, such as mixers, for bioreactors used on a laboratory scale, which ensure efficient turbulent mixing of the reaction liquid.
  • The disadvantage here is that with different sized bioreactors of different size mixers are needed to reduce or avoid up-scale problems. The provision of mixers in different sizes is relatively expensive.
  • From the DE 10 2004 013 078 A1 is a bioreactor with a vibratory mixer whose shaft (rod) with the flexible container wall of the bioreactor fluid-tightly connected by welding or gluing known. When occurring mixing problems / stagnant dead spaces without mixing, as can occur even with scale-ups, it is proposed to arrange several mixing devices of equal or smaller size in the bioreactor.
  • Furthermore, from the DE 100 16 554 A1 a bioreactor is known which has a dimensionally stable cover part, which carries the various supply and discharge lines and a support for cells and consists of a pot- or bag-shaped plastic tube whose opening is sealed and fixed against the lid. The arrangement of cell carriers or carrier plates, there is no room for a central mixer. Nutrient solution is fed to the interior of the bioreactor via a central feed tube, on which the plates are arranged radially, this feed preferably consisting of a plurality of short tube pieces to which correspondingly wide plates are fastened and the tube pieces are connected by intermediate pieces which serve as outlet nozzles for serve the nutrient fluid. Alternatively, this can also be done via separate supply lines in the outer region between the radial plates, an injection of nutrient solution. The supply and discharge lines consist of short pieces of pipe with intermediate binding pieces in order to easily assemble in length and thus in capacity variable bioreactors can. The disadvantage here is that the individual pieces of pipe or components are assembled via special connectors.
  • From the DE 32 45 556 A1 a column device for carrying out mass transfer processes is known, which consists of a vertical cylindrical housing, which is divided by means of horizontal partitions into several column stages through which a driven, centrally arranged shaft is guided, fixed to the non-rotatable fittings (eg stirring blades) are that circulate in the individual column stages in horizontal planes. Each horizontal partition wall consists of a rotationally fixed to the shaft seated circular disc and from this with a clear distance encompassing stationary annular disc.
  • From the DE 20 61 882 A is a multi-stirrer with a stirrer shaft and at this perpendicular to the agitator shaft axis fixed at an angle to the vertical plane stirring arms known. At the ends of the stirring arms stirring blades are arranged at an angle to the Rührarmachse. The stirring shaft axis carrying a plurality of stirring arms is formed in one piece.
  • Furthermore, from the GB 2 202 549 A a fermenter with a flexible wall and a rigid external support and a mechanical stirring device is known, which has on a stirring axis two spaced-apart stirrer.
  • It is therefore an object of the present invention to be able to provide differently sized bioreactors that are cost-effective and effective for scaling up.
  • This object is achieved in conjunction with the preamble of claim 1, characterized in that the mixer for variable adaptation to different sizes of the reactor interior of components standardized in size is composed, that the mixer shaft of a plurality of interlocking shaft elements can be assembled and that the individual shaft elements have a female connection part at one end and a male connection part which can be inserted into a female connection part at the opposite end.
  • The fact that the mixer is composed of components standardized in size, it can be used inexpensively and effectively not only in bioreactors with relatively small reactor interior space but also in bioreactors on an enlarged scale and adapted to the reactor interior. This can be carried out by the use of standardized components with minimum storage costs particularly cost-effective and yet effective.
  • Depending on the height of the reactor interior used, the mixer used or its mixer shaft is composed of two or more interlocking shaft elements. The fact that the shaft elements are locked into one another, a simple and inexpensive installation without special tools is achieved.
  • According to a preferred embodiment of the invention, the mixer shaft for scaling up with a plurality of mixer elements is providable.
  • By using multiple mixing elements, the area of the mixing elements can be easily scaled up to a larger scale. Thus, according to a further preferred embodiment of the invention, the mixer consists of a first mixing element, which is arranged on a first end of the mixer shaft facing away from the ceiling part, and at least one further mixing element, which is arranged, for example, between two shaft elements. The arrangement of two mixing elements at a distance from each other on top of each other not only a larger surface of the mixing elements is achieved but also an optimized area distribution with correspondingly larger, d. H. especially higher reactor interior.
  • According to a further preferred embodiment of the invention, a receiving part is arranged on the ceiling part, to which the mixer can be coupled in the reactor interior and on which the drive can be coupled outwards.
  • For different mixer sizes and for different sized ceiling parts, a receiving part is sufficient, at the inside of the mixer and to the outside of the drive is easily coupled. The shaft element can also be locked to the receiving part.
  • The male connection part can be positively secured against rotation in the female connector part. This is particularly advantageous when using a rotary mixer or a stirrer. For this purpose, the male connection part on two opposite sides have a flattening, so that rotation is prevented by a corresponding inner shape of the female part. However, the male connection part can also be designed as a hexagon or hexagon or have lateral guides.
  • However, the mixer can also be designed as a vibratory mixer displaceable in longitudinal oscillations.
  • In accordance with a further preferred embodiment of the invention, at least one media supply unit which can be assembled from standardized components is provided.
  • Because the media supply unit is also composed of standardized components with, in particular, standardized overall lengths, the media supply opening can be optimally positioned relative to the mixer or its mixer elements.
  • The media supply unit can be assembled from a plurality of elements which can be locked into one another, wherein the individual elements have a female connection part at one end and a male connection part which can be inserted into a female connection part at the opposite end. According to a further preferred embodiment of the invention, in particular the media supply unit is designed as a gassing unit.
  • According to a further preferred embodiment of the invention, at least one baffle which can be assembled from standardized components is arranged in the interior of the reactor.
  • The chicane is composed of a plurality of interlocking elements, the individual elements having at one end a female connection part and at the opposite end a male connection part insertable into a female connection part.
  • Further details of the invention will become apparent from the following detailed description and the accompanying drawings, in which preferred embodiments of the invention are illustrated by way of example.
  • In the drawings show:
  • 1 a side view of a bioreactor with mixer and two opposing baffles;
  • 2 : a side view of a bioreactor with one opposite 1 enlarged reactor interior with additional mixing element and additional baffle surface;
  • 3 a side view of another bioreactor with opposite 1 enlarged reactor interior, a vibration mixer with an additional mixing element and additional baffle surface;
  • 4 a side view of a shaft member in an enlarged view;
  • 5 : A side view of two interlocked shaft elements in half section and Ausriss in an enlarged view; and
  • 6 : An enlarged view of a mixing element with connector.
  • A bioreactor 1 consists essentially of a reactor interior 2 in which a mixer 3 from standardized components 4 is arranged.
  • The bioreactor 1 in the example is designed as a disposable bioreactor and defines the reactor interior 2 with a flexible side wall 5 , a flexible floor section 6 and a flexible ceiling part 7 ,
  • The one from the standardized components 4 composite mixers 3 consists of a mixer element 8th , a mixed race 9 and one outside the reactor interior 2 arranged drive 10 , The mix shaft 9 according to the embodiment of 1 consists of a first shaft element 11 that has a locking connection 12 with a second shaft element 13 connected is. The second shaft element 13 is via a locking connection 12 with one on the mixer element 8th arranged coupling part 14 of the mixer element 8th connected ( 6 ).
  • At the ceiling part 7 is a recording part 15 arranged, which has a rotatable connection between the drive axle 16 of the drive 10 and the mix shaft 9 manufactures. For this purpose, the connection part 15 in the reactor interior 2 a catch approach 17 on, with the free end of the first shaft element 11 a locking connection 18 forms. Accordingly, on the reactor interior 2 opposite outside 19 a locking connection 20 for connecting the drive axle 16 over the receiving part 15 with the first shaft element 11 intended.
  • The shaft elements 11 . 13 have a female connector at one end 21 and at the opposite end into a female connector 21 insertable male connector 22 on. The connecting parts 21 . 22 two interconnected shaft elements 11 . 13 form the locking connection 12 ( 4 and 5 ).
  • The mixer 3 in the embodiment of 1 is designed as a rotary mixer, which is the mixer element 8th around the shaft longitudinal axis 23 of the mix shaft 9 rotates.
  • The bioreactor 1 has one in his reactor interior 2 Sufficient media supply unit 24 also made of standardized components 25 is composable. The media feed unit 24 points to the ceiling part 7 to the reactor interior 2 towards a female connector 26 and to the outside a male connector 27 on. To the female connector 26 is a pipe element 28 with his male connection part 27 connected. The female connection part 26 of the tubular element 28 is then with a corresponding male connector 27 a gassing element 29 connectable. To the outer male connector 27 of the ceiling part 7 is a gas supply line 30 with a corresponding female connector 26 connected.
  • The bioreactor 1 points in his reactor interior 2 the flexible side wall 5 adjacent two opposing harassment 31 also made of standardized components 32 are composed. The chicane 31 has a first rod element 33 on, with one end to the ceiling part 7 is locked. With his the ceiling part 7 remote from the second end is the first rod member having a first end of a second rod member 34 locked. At the first rod element 33 remote from the second end is the second rod member 34 with a harassment element 35 locked, that the actual harassment area 36 wearing.
  • At the ceiling part 7 is another media connection 37 and at the bottom part 6 is a media connection 38 arranged.
  • The bioreactor 1' according to 2 points opposite to the bioreactor 1 in 1 a larger reactor interior 2 ' on. Accordingly, at the mixer shaft 9 ' of the mixer 3 ' an additional mixer element 8th' arranged. The harassment 31 ' have an additional baffle element 35 ' on.
  • The bioreactor 1'' according to the embodiment of 3 has a reactor interior 2 '' in which a trained as a vibration mixer mixer 3 '' is arranged. The mixer 3 '' has two mixer elements 8th'' . 8th''' at his mix shaft 9 ' on. Between the two mixer elements 8th'' . 8th''' is a second shaft element 13 '' arranged the distance between the two mixer elements 8th'' . 8th''' increased. Accordingly, the harassment elements 35 '' through a rod element 34 ''' spaced. A gassing element 29 '' is over two pipe elements 28 '' on the ceiling part 7 '' attached.

Claims (12)

  1. Bioreactor ( 1 ), in particular disposable bioreactor with flexible walls, with one of a side wall ( 5 ), a bottom part ( 6 ) and a ceiling part ( 7 . 7 '' ) bounded reactor interior ( 2 . 2 ' . 2 '' ), in which at least one mixer ( 3 . 3 ' . 3 '' ) consisting of at least one mixing element ( 8th . 8th'' ) and one on the ceiling part ( 7 . 7 '' ) arranged mixer shaft ( 9 . 9 ' . 9 '' ) from outside the reactor interior ( 2 . 2 ' . 2 '' ) arranged drive is drivable ( 10 ), characterized in that the mixer ( 3 . 3 ' . 3 '' ) for variable adaptation to different sizes of the reactor interior ( 2 . 2 ' . 2 '' ) of standardized components ( 4 ), that the mix of mixes ( 9 . 9 ' . 9 '' ) of a plurality of interlocking shaft elements ( 11 . 13 . 13 '' ) is composable and that the individual shaft elements on ( 11 . 11 '' . 13 . 13 '' ) one end a female connector ( 21 ) and at the opposite end into a female connector ( 21 ) insertable male connector ( 22 ) exhibit.
  2. Bioreactor according to claim 1, characterized in that the mixer shaft ( 9 ' . 9 '' ) for scaling up with a plurality of mixing elements ( 8th . 8th' . 8th'' . 8th''' ) is foreseeable.
  3. Bioreactor according to claim 2, characterized in that the mixer ( 3 '' ) from a first mixing element ( 8th'' ), which at its first part of the ceiling ( 7 '' ) facing away from the end of the mixer shaft ( 9 '' ) is arranged, and at least one further between two shaft elements ( 11 '' . 13 '' ) arranged mixing element ( 8th''' ) is composed.
  4. Bioreactor according to one of claims 2 to 3, characterized in that on the ceiling part ( 7 ) a receiving part ( 15 ) is arranged, on which in the reactor interior ( 2 ) the mixer ( 3 ) can be coupled and on the outside of the drive ( 10 ) can be coupled.
  5. Bioreactor according to claim 1, characterized in that the male connection part ( 22 ) in the female connector ( 21 ) is positively secured against rotation.
  6. Bioreactor according to one of claims 1 to 5, characterized in that the mixer ( 3 . 3 ' ) is designed as a rotary mixer.
  7. Bioreactor according to one of claims 1 to 6, characterized in that the mixer ( 3 '' ) is designed as a vibratory mixer displaceable in longitudinal oscillations.
  8. Bioreactor according to one of claims 1 to 7, characterized in that at least one of components standardized in size ( 25 ) composable media feed unit ( 24 ) is provided.
  9. Bioreactor according to claim 8, characterized in that the media supply unit ( 24 ) of a plurality of interlocking elements ( 26 . 27 . 29 . 30 ) is composable and that the individual element ( 25 . 27 . 29 . 30 ) at one end a female connector ( 21 ) and at the opposite end into a female connector ( 21 ) insertable male connector ( 22 ) exhibit.
  10. Bioreactor according to claim 8 or 9, characterized in that the media supply unit ( 24 ) is designed as a gassing unit.
  11. Bioreactor according to one of claims 1 to 10, characterized in that in the reactor interior at least one of largely standardized components ( 32 ) composable chicane ( 31 . 31 ' ) is arranged.
  12. Bioreactor according to claim 11, characterized in that the chicane ( 31 . 31 ' ) of a plurality of interlocking elements ( 33 . 34 . 35 ) is composable and that the individual elements at one end a female connector ( 21 ) and at the opposite end into a female connector ( 21 ) insertable male connector ( 22 ) exhibit.
DE200610021984 2006-05-10 2006-05-10 bioreactor Expired - Fee Related DE102006021984B4 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE200610021984 DE102006021984B4 (en) 2006-05-10 2006-05-10 bioreactor

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE200610021984 DE102006021984B4 (en) 2006-05-10 2006-05-10 bioreactor
DE200720005868 DE202007005868U1 (en) 2006-05-10 2007-04-24 One-way bioreactor for cultivating cells, includes flexible walls, side wall, bottom part and cover part limiting the reactor interior, in which rotation mixer comprising mixing element and mixer shank arranged at the cover part is arranged

Publications (2)

Publication Number Publication Date
DE102006021984A1 DE102006021984A1 (en) 2007-11-15
DE102006021984B4 true DE102006021984B4 (en) 2013-05-08

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DE200610021984 Expired - Fee Related DE102006021984B4 (en) 2006-05-10 2006-05-10 bioreactor
DE200720005868 Expired - Lifetime DE202007005868U1 (en) 2006-05-10 2007-04-24 One-way bioreactor for cultivating cells, includes flexible walls, side wall, bottom part and cover part limiting the reactor interior, in which rotation mixer comprising mixing element and mixer shank arranged at the cover part is arranged

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DE200720005868 Expired - Lifetime DE202007005868U1 (en) 2006-05-10 2007-04-24 One-way bioreactor for cultivating cells, includes flexible walls, side wall, bottom part and cover part limiting the reactor interior, in which rotation mixer comprising mixing element and mixer shank arranged at the cover part is arranged

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DE102017114886A1 (en) 2017-07-04 2019-01-10 Sartorius Stedim Biotech Gmbh Bioreactor with a fastening device

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US8870443B2 (en) 2008-04-17 2014-10-28 Sartorius Stedim Biotech Gmbh Flexible pouch with a mixing apparatus
DE102008025507A1 (en) 2008-05-28 2009-12-03 Sartorius Stedim Biotech Gmbh Mixing system
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US20140329224A1 (en) 2011-10-10 2014-11-06 DASGIP Information and Process Technology GmbH Biotechnological apparatus comprising a bioreactor, exhaust gas temperature control device for a bioreactor and a method for treating an exhaust gas stream in a biotechnological apparatus
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EP2853584A1 (en) 2013-09-30 2015-04-01 Eppendorf Ag Single-use bioreactor with baffle and a process for manufacturing and operating the same
WO2016002492A1 (en) * 2014-07-01 2016-01-07 佐竹化学機械工業株式会社 Reciprocating stirrer device with microbubble-generating unit
DE102014117658A1 (en) 2014-12-02 2016-06-02 Sartorius Stedim Biotech Gmbh Mixing device with flow breaker
FR3039776B1 (en) * 2015-08-03 2017-08-25 Sartorius Stedim Fmt Sas Method for assembling a container-mixer comprising a telescopic tree
MX2018014681A (en) * 2016-06-03 2019-06-06 Lonza Ag Single use bioreactor.
CN107497333A (en) * 2017-10-12 2017-12-22 江苏广鸿环境工程有限公司 A kind of efficient jacking stirrer

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US3415654A (en) * 1965-06-10 1968-12-10 Falstaff Brewing Corp Deep vat fermentation of beer
DD90533A5 (en) * 1970-07-21 1972-06-12 Ici Ltd Method and device for fermenting
DE2061882A1 (en) * 1970-12-16 1972-06-22 Hoesch & Soehne Eberhard Multi-stream stirrer assembly - with variable angle of incidence blades
DE3245556A1 (en) * 1982-10-22 1984-04-26 Albert Dipl Ing Moegli Column device for carrying out matter exchange processes or the like
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102017114886A1 (en) 2017-07-04 2019-01-10 Sartorius Stedim Biotech Gmbh Bioreactor with a fastening device

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