DD220334A5 - Verfahren zur isolierung des carboxypeptidase b enzyms aus dem pankreas von saeugetieren - Google Patents
Verfahren zur isolierung des carboxypeptidase b enzyms aus dem pankreas von saeugetieren Download PDFInfo
- Publication number
- DD220334A5 DD220334A5 DD84261731A DD26173184A DD220334A5 DD 220334 A5 DD220334 A5 DD 220334A5 DD 84261731 A DD84261731 A DD 84261731A DD 26173184 A DD26173184 A DD 26173184A DD 220334 A5 DD220334 A5 DD 220334A5
- Authority
- DD
- German Democratic Republic
- Prior art keywords
- centrifuged
- suspension
- allowed
- enzyme
- stand
- Prior art date
Links
- 210000000496 pancreas Anatomy 0.000 title claims abstract description 34
- 108090000087 Carboxypeptidase B Proteins 0.000 title claims description 34
- 102000003670 Carboxypeptidase B Human genes 0.000 title claims description 34
- 238000000034 method Methods 0.000 title claims description 15
- 239000000725 suspension Substances 0.000 claims abstract description 70
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims abstract description 52
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims abstract description 52
- 235000011130 ammonium sulphate Nutrition 0.000 claims abstract description 52
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 46
- 239000002244 precipitate Substances 0.000 claims abstract description 42
- 239000000203 mixture Substances 0.000 claims abstract description 21
- 102000004190 Enzymes Human genes 0.000 claims abstract description 19
- 108090000790 Enzymes Proteins 0.000 claims abstract description 19
- 239000000047 product Substances 0.000 claims abstract description 11
- 239000007853 buffer solution Substances 0.000 claims abstract description 8
- 238000010438 heat treatment Methods 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims description 39
- 239000006228 supernatant Substances 0.000 claims description 38
- 239000000872 buffer Substances 0.000 claims description 34
- 241000124008 Mammalia Species 0.000 claims description 4
- 238000000502 dialysis Methods 0.000 abstract description 20
- 239000012530 fluid Substances 0.000 abstract description 11
- 239000001166 ammonium sulphate Substances 0.000 abstract description 5
- 102000005367 Carboxypeptidases Human genes 0.000 abstract description 4
- 108010006303 Carboxypeptidases Proteins 0.000 abstract description 4
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 abstract 1
- 238000005086 pumping Methods 0.000 abstract 1
- 239000010414 supernatant solution Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 41
- 239000000243 solution Substances 0.000 description 33
- 235000018102 proteins Nutrition 0.000 description 27
- 102000004169 proteins and genes Human genes 0.000 description 27
- 108090000623 proteins and genes Proteins 0.000 description 27
- 239000012153 distilled water Substances 0.000 description 20
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 18
- 229920006395 saturated elastomer Polymers 0.000 description 17
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 16
- 229940088598 enzyme Drugs 0.000 description 16
- GFLCPYUSPYXNBV-NSHDSACASA-N (2s)-2-[(2-benzamidoacetyl)amino]-5-(diaminomethylideneamino)pentanoic acid Chemical compound NC(N)=NCCC[C@@H](C(O)=O)NC(=O)CNC(=O)C1=CC=CC=C1 GFLCPYUSPYXNBV-NSHDSACASA-N 0.000 description 15
- 235000013372 meat Nutrition 0.000 description 12
- 239000008363 phosphate buffer Substances 0.000 description 10
- 210000000056 organ Anatomy 0.000 description 7
- 239000000843 powder Substances 0.000 description 7
- 239000004475 Arginine Substances 0.000 description 6
- 229960003121 arginine Drugs 0.000 description 6
- GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 235000015277 pork Nutrition 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 238000000746 purification Methods 0.000 description 5
- 239000000758 substrate Substances 0.000 description 5
- 241000283707 Capra Species 0.000 description 4
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 4
- 239000006286 aqueous extract Substances 0.000 description 4
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 4
- 238000004587 chromatography analysis Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000283690 Bos taurus Species 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 239000012535 impurity Substances 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 208000035404 Autolysis Diseases 0.000 description 2
- 206010057248 Cell death Diseases 0.000 description 2
- 229930064664 L-arginine Natural products 0.000 description 2
- 235000014852 L-arginine Nutrition 0.000 description 2
- 229920005654 Sephadex Polymers 0.000 description 2
- 239000012507 Sephadex™ Substances 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 239000008351 acetate buffer Substances 0.000 description 2
- 235000009697 arginine Nutrition 0.000 description 2
- RQPZNWPYLFFXCP-UHFFFAOYSA-L barium dihydroxide Chemical compound [OH-].[OH-].[Ba+2] RQPZNWPYLFFXCP-UHFFFAOYSA-L 0.000 description 2
- 229910001863 barium hydroxide Inorganic materials 0.000 description 2
- 238000005194 fractionation Methods 0.000 description 2
- 210000004907 gland Anatomy 0.000 description 2
- 238000005342 ion exchange Methods 0.000 description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N lysine Chemical compound NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
- 230000028043 self proteolysis Effects 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 1
- -1 CM-cellulose ion Chemical class 0.000 description 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
- 102100035024 Carboxypeptidase B Human genes 0.000 description 1
- 102000005575 Cellulases Human genes 0.000 description 1
- 108010084185 Cellulases Proteins 0.000 description 1
- 101000946524 Homo sapiens Carboxypeptidase B Proteins 0.000 description 1
- 108010093096 Immobilized Enzymes Proteins 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-N L-arginine Chemical compound OC(=O)[C@@H](N)CCCN=C(N)N ODKSFYDXXFIFQN-BYPYZUCNSA-N 0.000 description 1
- 235000010676 Ocimum basilicum Nutrition 0.000 description 1
- 240000007926 Ocimum gratissimum Species 0.000 description 1
- 240000004713 Pisum sativum Species 0.000 description 1
- 235000010582 Pisum sativum Nutrition 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 229940024606 amino acid Drugs 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical group 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 235000013351 cheese Nutrition 0.000 description 1
- 238000011097 chromatography purification Methods 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 238000011033 desalting Methods 0.000 description 1
- 229940079919 digestives enzyme preparation Drugs 0.000 description 1
- 229940110377 dl- arginine Drugs 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- HWJHWSBFPPPIPD-UHFFFAOYSA-N ethoxyethane;propan-2-one Chemical compound CC(C)=O.CCOCC HWJHWSBFPPPIPD-UHFFFAOYSA-N 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 150000007522 mineralic acids Chemical class 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 235000012771 pancakes Nutrition 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 210000003079 salivary gland Anatomy 0.000 description 1
- 235000013580 sausages Nutrition 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000013616 tea Nutrition 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/814—Enzyme separation or purification
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/814—Enzyme separation or purification
- Y10S435/816—Enzyme separation or purification by solubility
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Dispersion Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| HU832469A HU190129B (en) | 1983-07-11 | 1983-07-11 | Process for the isolation of carboxypeptidase b enzyme from mammal pancreas |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DD220334A5 true DD220334A5 (de) | 1985-03-27 |
Family
ID=10959530
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DD84261731A DD220334A5 (de) | 1983-07-11 | 1984-04-06 | Verfahren zur isolierung des carboxypeptidase b enzyms aus dem pankreas von saeugetieren |
| DD84270845A DD222632A5 (de) | 1983-07-11 | 1984-04-06 | Verfahren zur immobilisierung von carboxypeptidase-b-enzym |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DD84270845A DD222632A5 (de) | 1983-07-11 | 1984-04-06 | Verfahren zur immobilisierung von carboxypeptidase-b-enzym |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US4650762A (cs) |
| CS (1) | CS252472B2 (cs) |
| CU (1) | CU21674A3 (cs) |
| DD (2) | DD220334A5 (cs) |
| DE (1) | DE3412669A1 (cs) |
| HU (1) | HU190129B (cs) |
| RO (1) | RO88619A (cs) |
| RU (1) | RU1769763C (cs) |
| SU (1) | SU1487817A3 (cs) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5278284A (en) * | 1992-05-14 | 1994-01-11 | Miller Brewing Company | Protein purification method |
| IL116696A (en) * | 1995-01-25 | 1999-08-17 | Bio Technology General Corp | Production of enzymatically active recombinant carboxypeptidase b |
| CN1193278A (zh) * | 1995-08-16 | 1998-09-16 | 曾尼卡有限公司 | 化合物 |
| RU2177997C1 (ru) * | 2000-05-12 | 2002-01-10 | Институт биоорганической химии имени М.М. Шемякина и Ю.А. Овчинникова РАН | Способ выделения карбоксипептидазы в |
| RU2354696C1 (ru) * | 2007-08-07 | 2009-05-10 | Федеральное государственное учреждение науки Государственный научный центр прикладной микробиологии и биотехнологии (ФГУН ГНЦ ПМБ) | Способ получения карбоксипептидазы b из поджелудочной железы свиньи |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3260654A (en) * | 1963-03-22 | 1966-07-12 | Ormonoterapia Richter Spa | Method for the preparation of a pancreas extract having a high proteolytic activity |
| FR3071M (fr) * | 1963-10-18 | 1965-01-18 | Centre Nat Rech Scient | Nouvelles protéases pancréatiques, utilisables notamment en gastro-entérologie. |
| US3625829A (en) * | 1967-12-04 | 1971-12-07 | Bayer Ag | Purification of carboxypeptidase b |
| JPS57150384A (en) * | 1981-03-12 | 1982-09-17 | Eisai Co Ltd | Carboxypeptidase ar and its preparation |
| US4532214A (en) * | 1982-06-03 | 1985-07-30 | Reanal Finomvegyszergyar | Method for isolation of aminoacylase |
-
1983
- 1983-07-11 HU HU832469A patent/HU190129B/hu not_active IP Right Cessation
-
1984
- 1984-03-29 CS CS842361A patent/CS252472B2/cs unknown
- 1984-04-02 US US06/595,767 patent/US4650762A/en not_active Expired - Fee Related
- 1984-04-04 DE DE19843412669 patent/DE3412669A1/de not_active Ceased
- 1984-04-05 RO RO84114181A patent/RO88619A/ro unknown
- 1984-04-06 DD DD84261731A patent/DD220334A5/de not_active IP Right Cessation
- 1984-04-06 DD DD84270845A patent/DD222632A5/de not_active IP Right Cessation
- 1984-04-16 SU SU843731150A patent/SU1487817A3/ru active
- 1984-07-11 CU CU832469/83A patent/CU21674A3/es unknown
-
1987
- 1987-01-13 RU SU874028800A patent/RU1769763C/ru active
Also Published As
| Publication number | Publication date |
|---|---|
| CS236184A2 (en) | 1987-01-15 |
| HUT34234A (en) | 1985-02-28 |
| CS252472B2 (en) | 1987-09-17 |
| RO88619A (ro) | 1986-02-28 |
| DD222632A5 (de) | 1985-05-22 |
| DE3412669A1 (de) | 1985-01-24 |
| HU190129B (en) | 1986-08-28 |
| CU21674A3 (en) | 1987-10-12 |
| SU1487817A3 (ru) | 1989-06-15 |
| US4650762A (en) | 1987-03-17 |
| RU1769763C (ru) | 1992-10-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| ENJ | Ceased due to non-payment of renewal fee |