CY1116252T1 - IMMUNE PCV2 COMPOSITIONS FOR USE IN PCV2 INFECTION METHOD - Google Patents
IMMUNE PCV2 COMPOSITIONS FOR USE IN PCV2 INFECTION METHODInfo
- Publication number
- CY1116252T1 CY1116252T1 CY20151100406T CY151100406T CY1116252T1 CY 1116252 T1 CY1116252 T1 CY 1116252T1 CY 20151100406 T CY20151100406 T CY 20151100406T CY 151100406 T CY151100406 T CY 151100406T CY 1116252 T1 CY1116252 T1 CY 1116252T1
- Authority
- CY
- Cyprus
- Prior art keywords
- pcv2
- reading frame
- open reading
- cells
- expressed
- Prior art date
Links
Abstract
Παρέχεται βελτιωμένη μέθοδος για ανάκτηση της πρωτεΐνης που εκφράζεται από το ανοικτό πλαίσιο ανάγνωσης (2) από τον χοίρειο κυκλοϊό τύπου (2). Η μέθοδος περιλαμβάνει γενικώς τις βαθμίδες επιμόλυνσης του ανασυνδυαστικού ιού που περιέχει τις ακολουθίες κωδικοποίησης του ανοικτού πλαισίου ανάγνωσης (2) μέσα σε κύτταρα, τα οποία περιέχονται σε μέσα αύξησης, προκαλώντας τον ιό να εκφράσει το ανοικτό πλαίσιο ανάγνωσης (2), καθώς και της ανάκτησης της πρωτεΐνης που εκφράζεται στο υπερκείμενο. Αυτή η ανάκτηση πρέπει να λαμβάνει χώρα αρχίζοντας κατά προσέγγιση (5) ημέρες μετά τη λοίμωξη των κυττάρων, προκειμένου να επιτραπεί σε επαρκείς ποσότητες της ανασυνδυαστικής πρωτεΐνης να εκφραστούν και να εκκριθούν από το κύτταρο στα μέσα αύξησης. Οι μέθοδοι αυτές αποφεύγουν τις δαπανηρές και χρονοβόρες διαδικασίες εξαγωγής, οι οποίες απαιτούνται για διαχωρισμό και ανάκτηση της ανασυνδυαστικής πρωτεΐνης μέσα από τα κύτταρα.An improved method is provided for recovering the protein expressed from the open reading frame (2) by the porcine type (2). The method generally comprises the steps of transfection of the recombinant virus containing the coding sequences of the open reading frame (2) into cells contained in growth media, causing the virus to express the open reading frame (2), as well as the recovery. of the protein expressed in the supernatant. This recovery should take place approximately (5) days after the infection of the cells, in order to allow sufficient amounts of the recombinant protein to be expressed and secreted by the cell in growth media. These methods avoid the expensive and time-consuming extraction processes required to separate and recover the recombinant protein within the cells.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US64051004A | 2004-12-30 | 2004-12-30 | |
| US11/034,797 US7833707B2 (en) | 2004-12-30 | 2005-01-13 | Methods of overexpression and recovery of porcine circovirus type 2 ORF2 |
| US11/319,975 US7700285B1 (en) | 2005-12-29 | 2005-12-29 | PCV2 immunogenic compositions and methods of producing such compositions |
| EP05856068.1A EP1833992B1 (en) | 2004-12-30 | 2005-12-29 | Pcv2 immunogenic compositions and methods of producing such compositions |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CY1116252T1 true CY1116252T1 (en) | 2017-03-15 |
Family
ID=58459721
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CY20151100255T CY1116184T1 (en) | 2004-12-30 | 2015-03-13 | IMMUNE PCV2 COMPOSITIONS FOR USE IN PCV2 INFECTION METHOD |
| CY20151100406T CY1116252T1 (en) | 2004-12-30 | 2015-05-08 | IMMUNE PCV2 COMPOSITIONS FOR USE IN PCV2 INFECTION METHOD |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CY20151100255T CY1116184T1 (en) | 2004-12-30 | 2015-03-13 | IMMUNE PCV2 COMPOSITIONS FOR USE IN PCV2 INFECTION METHOD |
Country Status (1)
| Country | Link |
|---|---|
| CY (2) | CY1116184T1 (en) |
-
2015
- 2015-03-13 CY CY20151100255T patent/CY1116184T1/en unknown
- 2015-05-08 CY CY20151100406T patent/CY1116252T1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CY1116184T1 (en) | 2017-03-15 |
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