CY1115538T1 - SPECIFIC TO RNA FOLLOWING RNA INTRODUCTION MEDIATORS - Google Patents
SPECIFIC TO RNA FOLLOWING RNA INTRODUCTION MEDIATORSInfo
- Publication number
- CY1115538T1 CY1115538T1 CY20141100724T CY141100724T CY1115538T1 CY 1115538 T1 CY1115538 T1 CY 1115538T1 CY 20141100724 T CY20141100724 T CY 20141100724T CY 141100724 T CY141100724 T CY 141100724T CY 1115538 T1 CY1115538 T1 CY 1115538T1
- Authority
- CY
- Cyprus
- Prior art keywords
- specific
- fragments
- rna
- drosophila
- mediators
- Prior art date
Links
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- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Η παρούσα εφεύρεση αφορά κάποιο in vitro σύστημα δροσόφιλας (Drosophila) το οποίο χρησιμοποιήθηκε για να αποδειχθεί ότι dsRΝΑ έχει μετατραπεί σε τμήματα RΝΑ μήκους 21-23 νουκλεοτιδίων (nt). Επιπλέον, όταν τα συγκεκριμένα θραύσματα 21-23 nt καθαριστούν και προστεθούν πάλι στα εκχυλίσματα της δροσόφιλας, διαμεσολαβούν στην παρεμβολή RΝΑ απουσία του μακριού dsRΝΑ. Συνεπώς, τα συγκεκριμένα θραύσματα των 21-23 nt είναι ειδικοί ως προς την ακολουθία, μεσολαβητές της αποικοδόμησης RΝΑ. Στα συγκεκριμένα θραύσματα των 21-23 nt πρέπει να υπάρχει κάποιο μοριακό σήμα, το οποίο μπορεί να είναι το συγκεκριμένο μήκος των θραυσμάτων, τα οποία προσελκύουν τους κυτταρικούς παράγοντες που συμμετέχουν στην RΝΑi. Η παρούσα εφεύρεση περιλαμβάνει τα συγκεκριμένα θραύσματα των 21-23 nt και τη χρήση τους για την ειδική αδρανοποίηση της γονιδιακής λειτουργίας. Η χρήση αυτών των θραυσμάτων (ή χημικώς συντιθέμενων ολιγονουκλεοτιδίων ίδιας ή παρόμοιας φύσης) επιτρέπει τη στόχευση των ειδικών mRΝΑ που προορίζονται για να αποικοδομηθούν σε κύτταρα θηλαστικών, όπου η χρήση μακρών dsRΝΑ για την έκλυση RΝΑi συνήθως δεν είναι πρακτική, πιθανόν λόγω των επιβλαβών επιδράσεων της απάντησης της ιντερφερόνης. Αυτή η ειδική στόχευση κάποιας συγκεκριμένης γονιδιακής λειτουργίας είναι χρήσιμη σε θεραπευτικές εφαρμογές και των λειτουργιών του γονιδιώματος.The present invention relates to an in vitro Drosophila system (Drosophila) used to demonstrate that dsRNA has been converted to 21-23 nucleotide (nt) RNA segments. In addition, when these 21-23 nt fragments are purified and added back to the drosophila extracts, they mediate RNA interference in the absence of long dsRNA. Therefore, these 21-23 nt specific fragments are sequence-specific, mediators of RNA degradation. In particular 21-23 nt fragments there must be a molecular signal, which may be the specific length of the fragments, which attract the cellular factors involved in RNAi. The present invention encompasses specific fragments of 21-23 nt and their use for specific inactivation of gene function. The use of these fragments (or chemically synthesized oligonucleotides of the same or similar nature) allows targeting of specific mRNAs intended for degradation in mammalian cells, where the use of long dsRNAs to release RNAs is usually not practical because of response to interferon. This specific targeting of a particular gene function is useful in therapeutic applications and functions of the genome.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US19359400A | 2000-03-30 | 2000-03-30 | |
| EP00126325 | 2000-12-01 | ||
| US26523201A | 2001-01-31 | 2001-01-31 | |
| EP20100184711 EP2345742B1 (en) | 2000-03-30 | 2001-03-30 | RNA sequence-specific mediators of RNA interference |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CY1115538T1 true CY1115538T1 (en) | 2017-01-04 |
Family
ID=58436528
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CY20131100439T CY1114396T1 (en) | 2000-03-30 | 2013-06-03 | RNA Sequences Specific to RNA Interference Mediators |
| CY20141100345T CY1115228T1 (en) | 2000-03-30 | 2014-05-16 | RNA SPECIFIC TO THE FOLLOWING RNA INTERFERENCE MEDIATORS |
| CY20141100389T CY1115264T1 (en) | 2000-03-30 | 2014-05-30 | METHODS OF PRODUCTION OF CELLS OR ORGANIZATIONS WITH AN INHIBITED EXPRESSION OF ANY GENE (KNOCKDOWN) USING SPECIALLY INSTRUMENTABLE RNA |
| CY20141100724T CY1115538T1 (en) | 2000-03-30 | 2014-09-08 | SPECIFIC TO RNA FOLLOWING RNA INTRODUCTION MEDIATORS |
Family Applications Before (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CY20131100439T CY1114396T1 (en) | 2000-03-30 | 2013-06-03 | RNA Sequences Specific to RNA Interference Mediators |
| CY20141100345T CY1115228T1 (en) | 2000-03-30 | 2014-05-16 | RNA SPECIFIC TO THE FOLLOWING RNA INTERFERENCE MEDIATORS |
| CY20141100389T CY1115264T1 (en) | 2000-03-30 | 2014-05-30 | METHODS OF PRODUCTION OF CELLS OR ORGANIZATIONS WITH AN INHIBITED EXPRESSION OF ANY GENE (KNOCKDOWN) USING SPECIALLY INSTRUMENTABLE RNA |
Country Status (1)
| Country | Link |
|---|---|
| CY (4) | CY1114396T1 (en) |
-
2013
- 2013-06-03 CY CY20131100439T patent/CY1114396T1/en unknown
-
2014
- 2014-05-16 CY CY20141100345T patent/CY1115228T1/en unknown
- 2014-05-30 CY CY20141100389T patent/CY1115264T1/en unknown
- 2014-09-08 CY CY20141100724T patent/CY1115538T1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CY1115264T1 (en) | 2017-01-04 |
| CY1115228T1 (en) | 2017-01-04 |
| CY1114396T1 (en) | 2016-08-31 |
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