CU22642A1 - CDNA SEQUENCE DERIVED FROM THE GENE OF THE HEPATITIS C VIRUS AND ITS USE - Google Patents

CDNA SEQUENCE DERIVED FROM THE GENE OF THE HEPATITIS C VIRUS AND ITS USE

Info

Publication number
CU22642A1
CU22642A1 CU1996119A CU1996119A CU22642A1 CU 22642 A1 CU22642 A1 CU 22642A1 CU 1996119 A CU1996119 A CU 1996119A CU 1996119 A CU1996119 A CU 1996119A CU 22642 A1 CU22642 A1 CU 22642A1
Authority
CU
Cuba
Prior art keywords
hepatitis
virus
particle
development
hcv
Prior art date
Application number
CU1996119A
Other languages
Spanish (es)
Inventor
Rivero Nelson Acosta
Grillo Juan Morales
Carrera Santiago Duenas
Cabrera Odalys Garcia
Martinez Ciro Garcia
Rodriguez Ariel Vina
Alvarez Mario Livan Barro
Aizpurua Ivis Guerra
Cama Viviana Falcon
Original Assignee
Ct Ingenieria Genetica Biotech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ct Ingenieria Genetica Biotech filed Critical Ct Ingenieria Genetica Biotech
Priority to CU1996119A priority Critical patent/CU22642A1/en
Priority to AU53974/98A priority patent/AU5397498A/en
Priority to PCT/CU1997/000007 priority patent/WO1998025960A1/en
Publication of CU22642A1 publication Critical patent/CU22642A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención provee una serie de secuencias de ADNc derivadas del virus de la hepatitis C, denominado VHC-Cu, y la expresión de los antígenos codificados por dichas secuencias. El ARN viral del VHC-Cu se extrajo del suero de un paciente con hepatitis no-A, no-B. El ADNc se amplificó por PCR y los fragmentos obtenidos fueron clonados y secuenciados. Los fragmentos que codificaban para polipéptidos antigénicos fueron expresados en levadura, obteniéndose una proteína multimérica o una partícula semejante a virus. El análisis por microscopía electrónica mostró una partícula de 20 a 60 nm de diámetro, con densidad de flotación entre 1.16 y 1.30 g/cm3 en gradientes de sacarosa y cloruro de cesio. Esta invención puede ser utilizada en la medicina para el desarrollo de vacunas terapéuticas y profilácticas, para la producción de proteínas con interés diagnóstico, para el desarrollo de anticuerpos policlonales, anticuerpos monoclonales naturales o recombinantes, y agentes antivirales.The present invention provides a series of cDNA sequences derived from the hepatitis C virus, called HCV-Cu, and the expression of the antigens encoded by said sequences. The HCV-Cu viral RNA was extracted from the serum of a patient with non-A, non-B hepatitis. The cDNA was amplified by PCR and the fragments obtained were cloned and sequenced. The fragments coding for antigenic polypeptides were expressed in yeast, obtaining a multimeric protein or a virus-like particle. Analysis by electron microscopy showed a particle of 20 to 60 nm in diameter, with a float density between 1.16 and 1.30 g / cm3 in gradients of sucrose and cesium chloride. This invention can be used in medicine for the development of therapeutic and prophylactic vaccines, for the production of proteins of diagnostic interest, for the development of polyclonal antibodies, natural or recombinant monoclonal antibodies, and antiviral agents.

CU1996119A 1996-12-12 1996-12-12 CDNA SEQUENCE DERIVED FROM THE GENE OF THE HEPATITIS C VIRUS AND ITS USE CU22642A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CU1996119A CU22642A1 (en) 1996-12-12 1996-12-12 CDNA SEQUENCE DERIVED FROM THE GENE OF THE HEPATITIS C VIRUS AND ITS USE
AU53974/98A AU5397498A (en) 1996-12-12 1997-12-12 Sequences derived from the genome of the c hepatitis virus, and use thereof
PCT/CU1997/000007 WO1998025960A1 (en) 1996-12-12 1997-12-12 Sequences derived from the genome of the c hepatitis virus, and use thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU1996119A CU22642A1 (en) 1996-12-12 1996-12-12 CDNA SEQUENCE DERIVED FROM THE GENE OF THE HEPATITIS C VIRUS AND ITS USE

Publications (1)

Publication Number Publication Date
CU22642A1 true CU22642A1 (en) 2000-12-22

Family

ID=46093345

Family Applications (1)

Application Number Title Priority Date Filing Date
CU1996119A CU22642A1 (en) 1996-12-12 1996-12-12 CDNA SEQUENCE DERIVED FROM THE GENE OF THE HEPATITIS C VIRUS AND ITS USE

Country Status (3)

Country Link
AU (1) AU5397498A (en)
CU (1) CU22642A1 (en)
WO (1) WO1998025960A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CU23244A1 (en) * 2001-07-16 2007-10-17 Ct Ingenieria Genetica Biotech VACCINATION FORMULATION POTENTIATED BY THE COMBINATION OF A DNA WITH AN ANTIGEN
CA2566506A1 (en) * 2004-06-01 2005-12-15 Innogenetics N.V. Peptides for inducing a ctl and/or htl response to hepatitis c virus
CU23470A1 (en) 2004-08-11 2009-12-17 Ct Ingenieria Genetica Biotech RECOMBINANT LIVING VECTORS AND THEIR USE IN PHARMACEUTICAL COMPOSITIONS AGAINST THE VIRUS OF HEPATITIS C

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1074422C (en) * 1987-11-18 2001-11-07 希龙股份有限公司 Nanbv diagnostics and vaccines
CA2044296A1 (en) * 1990-06-12 1991-12-13 Hiroaki Okamoto Oligonucleotide primers, and their application for high-fidelity detection of non-a, non-b hepatitis virus
JP3268502B2 (en) * 1990-06-12 2002-03-25 徹雄 中村 Non-A non-B hepatitis virus-related polynucleotide, polypeptide
CU22290A1 (en) * 1990-10-08 1995-01-31 Cigb Procedure for obtaining surface antigens from the hepatitis b virus with a greater immunogenic capacity and its use in preparing a vaccine.
EP0787807B1 (en) * 1991-08-27 2003-05-28 F. Hoffmann-La Roche Ag Primers and probes for hepatitis C detection
EP0532258A2 (en) * 1991-09-09 1993-03-17 Immuno Japan Inc. Oligonucleotides and determination system of HCV genotypes
JPH06153961A (en) * 1992-11-27 1994-06-03 Imuno Japan:Kk Partial gene of hepatitis c virus and method for detecting gene of hepatitis c virus in high sensitivity
JP3472319B2 (en) * 1993-06-18 2003-12-02 株式会社先端生命科学研究所 Recombinant polypeptide comprising amino acid sequence of non-A non-B hepatitis virus antigen and method for detecting non-A non-B hepatitis virus antibody using said polypeptide
JPH07322881A (en) * 1994-05-31 1995-12-12 S R L:Kk Oligonucleotide, diagnostic reagent for hepatitis c comprising the same and method for diagnosing hepatitis c using the same

Also Published As

Publication number Publication date
AU5397498A (en) 1998-07-03
WO1998025960A1 (en) 1998-06-18

Similar Documents

Publication Publication Date Title
Rollier et al. Protective and therapeutic effect of DNA-based immunization against hepadnavirus large envelope protein
SE9004010D0 (en) VIRAL MEDICINE
DK1033995T3 (en) Whole or disrupted insect cells as adjuvants for antigens
FI935808A (en) Hepatitis C virus (HCV) polypeptide
EA200100425A1 (en) NEW METHODS OF THERAPEUTIC VACCINATION
BR9813416A (en) Compositions and methods of recombinant nodaviruses
DE69111801D1 (en) VACCINE AGAINST HEPATITIS B.
RU2319505C2 (en) Purified coat proteins of hepatitis c virus for diagnostic and therapeutic application
JPH0327400A (en) B-type hepatitis virus core antigen particle
Pumpens et al. Evaluation of HBs, HBc, and frCP virus-like particles for expression of human papillomavirus 16 E7 oncoprotein epitopes
DK461884A (en) PROTECTIVE PEPTID ANTIGEN
KR910019641A (en) Serum Reactive Epitopes on Proteins of Human Papilloma Virus (HPV) 18
KR880701283A (en) Virus vectors, vaccines, and antibodies that encode the glycoprotein of virus that causes AIDS
CA2098253C (en) Method for producing ectoprotein of hepatitis c virus
Feitelson et al. The nature of polypeptides larger in size than the major surface antigen components of hepatitis b and like viruses in ground squirrels, woodchucks, and ducks
EP2198020B1 (en) Polynucleotides allowing the expression and secretion of recombinant pseudo-virus containing foreign epitopes, their production, and use
CU22642A1 (en) CDNA SEQUENCE DERIVED FROM THE GENE OF THE HEPATITIS C VIRUS AND ITS USE
ATE290399T1 (en) HBV/HCV VIRUS-WITHOUT PARTICLES
BR9815912A (en) Vaccine-induced viral hepatitis B strain and its uses
Kos et al. Induction of primary anti‐viral cytotoxic T cells by in vitro stimulation with short synthetic peptide and interleukin‐7
DK35891A (en) PRACTICALLY TAKEN PURE, ANTIGENIC PEPTIDE OR PROTEIN WITH AN EPITOP OF HAEMOPHILUS INFLUENZAE, ITS PREPARATION AND USE
CU22290A1 (en) Procedure for obtaining surface antigens from the hepatitis b virus with a greater immunogenic capacity and its use in preparing a vaccine.
DE68926274D1 (en) Recombinant HBsAg hybrid particles which have morphological properties of HBsAg antigen and which contain an immunogenic sequence which induces neutralizing antibodies against HIV or which is recognized by these antibodies, nucleotide sequences which encode these particles and vaccines containing them
AR009855A1 (en) NUCLEOTIDIC SEQUENCES OF cDNA DERIVED FROM THE HEPATITIS C GENOME, RECOMBINANT POLYNUCLEOTIDES, TRANSFORMED MICROORGANISMS, PROTEIN SUBSTANCES, PROTEIN CRYSTALS AND A RECOMBINED MOLECULA
BASAK et al. Construction and characterization of encapsidated poliovirus replicons that express biologically active murine interleukin-2