CO5300499A1 - TEST OF IMMUNOABSORBENTLY LINKED TO INHIBITION ENZYME (ELISA) FOR DETECTION AND QUANTIFICATION OF ANTIBODIES IN BIOLOGICAL SAMPLES - Google Patents

TEST OF IMMUNOABSORBENTLY LINKED TO INHIBITION ENZYME (ELISA) FOR DETECTION AND QUANTIFICATION OF ANTIBODIES IN BIOLOGICAL SAMPLES

Info

Publication number
CO5300499A1
CO5300499A1 CO00076267A CO00076267A CO5300499A1 CO 5300499 A1 CO5300499 A1 CO 5300499A1 CO 00076267 A CO00076267 A CO 00076267A CO 00076267 A CO00076267 A CO 00076267A CO 5300499 A1 CO5300499 A1 CO 5300499A1
Authority
CO
Colombia
Prior art keywords
antibody
detection
immunoabsorbently
quantification
elisa
Prior art date
Application number
CO00076267A
Other languages
Spanish (es)
Inventor
Mark A Abrams
Original Assignee
Pharmacia Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmacia Corp filed Critical Pharmacia Corp
Publication of CO5300499A1 publication Critical patent/CO5300499A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Un método para la detección de un primer anticuerpo en una muestra de prueba, que comprende los pasos de:recubrir una superficie de enlazamiento o soporte con un segundo anticuerpo purificado para formar una superficie revestida con anticuerpo;combinar una cantidad predeterminada de un antígeno etiquetado purificado y dicha muestra de prueba que contiene dicho primer anticuerpo;agregar la mezcla incubada de antígeno y primer anticuerpo a dicha superficie revestida con anticuerpo;incubar dicha superficie recubierta con anticuerpo con dicha combinación de antígeno y primer anticuerpo; ymedir la cantidad de inhibición enlazante de antígeno.A method for the detection of a first antibody in a test sample, comprising the steps of: coating a binding or support surface with a second purified antibody to form an antibody coated surface; combining a predetermined amount of a purified labeled antigen and said test sample containing said first antibody; adding the incubated mixture of antigen and first antibody to said antibody coated surface; incubating said antibody coated surface with said combination of antigen and first antibody; and measure the amount of antigen binding inhibition.

CO00076267A 1999-10-07 2000-10-06 TEST OF IMMUNOABSORBENTLY LINKED TO INHIBITION ENZYME (ELISA) FOR DETECTION AND QUANTIFICATION OF ANTIBODIES IN BIOLOGICAL SAMPLES CO5300499A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US15809099P 1999-10-07 1999-10-07

Publications (1)

Publication Number Publication Date
CO5300499A1 true CO5300499A1 (en) 2003-07-31

Family

ID=22566639

Family Applications (1)

Application Number Title Priority Date Filing Date
CO00076267A CO5300499A1 (en) 1999-10-07 2000-10-06 TEST OF IMMUNOABSORBENTLY LINKED TO INHIBITION ENZYME (ELISA) FOR DETECTION AND QUANTIFICATION OF ANTIBODIES IN BIOLOGICAL SAMPLES

Country Status (4)

Country Link
AU (1) AU7825500A (en)
CO (1) CO5300499A1 (en)
PE (1) PE20010633A1 (en)
WO (1) WO2001027621A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102221616B (en) * 2011-04-15 2014-01-15 华南农业大学 Indirect ELISA (Enzyme-Linked Immuno Sorbent Assay) diagnostic kit of mycoplasma gallisepticum
CN103163302B (en) * 2011-12-10 2015-06-03 河北菲尼斯生物技术有限公司 Oligopeptide antibody kit prepared by directional cross-coupling technology
CN104122395A (en) * 2013-04-28 2014-10-29 北京协和洛克生物技术有限责任公司 Kit for detecting myeloperoxidase (MPO) concentration in sample and preparation method of kit
WO2015061260A1 (en) 2013-10-24 2015-04-30 Siemens Healthcare Diagnostics Inc. Assays for macromolecular analytes
CN111273044B (en) * 2020-02-20 2023-08-18 中国科学院亚热带农业生态研究所 Method for evaluating temperature and humidity state of environment where individual growth of nursery pigs is located

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5738849A (en) * 1992-11-24 1998-04-14 G. D. Searle & Co. Interleukin-3 (IL-3) variant fusion proteins, their recombinant production, and therapeutic compositions comprising them
EP0670898B1 (en) * 1992-11-24 2003-10-08 G.D. Searle & Co. Interleukin-3 (il-3) mutant polypeptides
PL184424B1 (en) * 1995-10-05 2002-10-31 Searle & Co Agonists of haemopoietic multifunctional receptors
GB9601081D0 (en) * 1995-10-06 1996-03-20 Cambridge Antibody Tech Specific binding members for human transforming growth factor beta;materials and methods
WO1997014795A1 (en) * 1995-10-19 1997-04-24 John Wayne Cancer Institute Tumor associated polypeptides with carboxyl-terminal alanine-proline, proline-proline or glycine-proline and antibodies thereto
WO1998016826A1 (en) * 1996-10-17 1998-04-23 The Dow Chemical Company Immunoassay for the detection and measurement of 2,2-dibromo-3-nitrilopropionamide
AU7977098A (en) * 1997-06-17 1999-01-25 Palingen, Inc. Method for diagnosing systemic lupus erythematosus

Also Published As

Publication number Publication date
WO2001027621A2 (en) 2001-04-19
AU7825500A (en) 2001-04-23
WO2001027621A3 (en) 2001-08-30
PE20010633A1 (en) 2001-06-11

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Legal Events

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