CN85108100A - The preparation method of tunica fibrosa, tunica fibrosa product, artificial leather implant piece and application thereof - Google Patents

The preparation method of tunica fibrosa, tunica fibrosa product, artificial leather implant piece and application thereof Download PDF

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Publication number
CN85108100A
CN85108100A CN 85108100 CN85108100A CN85108100A CN 85108100 A CN85108100 A CN 85108100A CN 85108100 CN85108100 CN 85108100 CN 85108100 A CN85108100 A CN 85108100A CN 85108100 A CN85108100 A CN 85108100A
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CN
China
Prior art keywords
tunica fibrosa
film
developing medium
dehydration
bacterium
Prior art date
Application number
CN 85108100
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Chinese (zh)
Other versions
CN1015908B (en
Inventor
鲁茨·弗尔南多·萨维·法拉
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比奥·菲尔医学产品工商有限公司
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Priority to BR8404937A priority Critical patent/BR8404937A/en
Priority to BR8404937 priority
Application filed by 比奥·菲尔医学产品工商有限公司 filed Critical 比奥·菲尔医学产品工商有限公司
Publication of CN85108100A publication Critical patent/CN85108100A/en
Publication of CN1015908B publication Critical patent/CN1015908B/en

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Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D71/00Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06Organic material
    • B01D71/08Polysaccharides
    • B01D71/10Cellulose; Modified cellulose
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
    • A61L27/00Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14Macromolecular materials
    • A61L27/20Polysaccharides
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
    • A61L27/00Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/60Materials for use in artificial skin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/04Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • DTEXTILES; PAPER
    • D06TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06NWALL, FLOOR, OR LIKE COVERING MATERIALS, e.g. LINOLEUM, OILCLOTH, ARTIFICIAL LEATHER, ROOFING FELT, CONSISTING OF A FIBROUS WEB COATED WITH A LAYER OF MACROMOLECULAR MATERIAL; FLEXIBLE SHEET MATERIAL NOT OTHERWISE PROVIDED FOR
    • D06N3/00Artificial leather, oilcloth or other material obtained by covering fibrous webs with macromolecular material, e.g. resins, rubber or derivatives thereof
    • D06N3/02Artificial leather, oilcloth or other material obtained by covering fibrous webs with macromolecular material, e.g. resins, rubber or derivatives thereof with cellulose derivatives
    • AHUMAN NECESSITIES
    • A63SPORTS; GAMES; AMUSEMENTS
    • A63BAPPARATUS FOR PHYSICAL TRAINING, GYMNASTICS, SWIMMING, CLIMBING, OR FENCING; BALL GAMES; TRAINING EQUIPMENT
    • A63B51/00Stringing tennis, badminton or like rackets; Strings therefor; Maintenance of racket strings
    • A63B51/02Strings; String substitutes; Products applied on strings, e.g. for protection against humidity or wear

Abstract

The invention describes the method for preparing tunica fibrosa, comprising the steps: to prepare with nitrogen and carbohydrate is the developing medium of nutrition source, a kind of genus acetobacter of inoculation in this developing medium, the bacterium of Xylinum kind; Make under the activated temperature of bacterium, in the time of the desired use that is fit to film, cultivating; From developing medium, take out the film that generates, under swelling state, dewater.Zhi Bei film is suitable for use as the artificial leather implant piece, is used as separatory membrane or imitation leather like this.

Description

The present invention discusses the artificial leather implant piece, more particularly provides a kind of method for preparing synthetic leather.

The serious problems that skin injury medically runs into often.Particularly burn, patient suffers a large amount of water-dielectric losses, and these losses increase along with the expansion of burn surface area.In burn district, about 36 hours that lose behind a large amount of blood is critical days.Spent critical days, lost the liquid situation owing to the sealing of tissue is interrupted, but opposite result takes place, i.e. retention owing to transudate produces swelling.Bigger as affected zone, its possibility of result is fatal.

It is serious causing the caused above-mentioned symptom of skin burn by thermal radiation or chemical agent.But as skin injury is that then symptom is lighter owing to accidental scratch or other mechanical reason.Traditional drug treating method, such as using ointment, the gauze that oils, and bandage and make gauze fixing etc., not only time-consuming but also misery arranged.Patient's injury will be wrapped up, and accepts heavy dose of antibiotic medicine in case infect.For many years, the problems referred to above are by adopting skin substitute products to obtain solution.The relevant document more than 120 pieces that published in nearly ten years in the whole world is the human proof of constantly seeking skin substitute products.These documents concentrate on attempts to seek character a kind of and fell the surrogate identical with feature.

Existing so far following several fell surrogates:

1. autotransplantation is other position of taking from this person of patient;

2. heteroplastic transplantation has two types:

The first, take from for the fell on skin person or the corpse, or take from the amnion that supplies the skin person;

The second, pigskin, ox embryo or artificial skin, as the mixture of polysiloxane, collagen, polysiloxane and collagen, or tetrafluoroethylene, urethane.

These methods can be described below in a capsule:

Autotransplantation is taken off a skin from one of patient oneself selected skin donor site by surgical operation and is spread on the wound district immediately.Because the operation peeling equals to cause second degree burn, so only be applicable to the patient of third degree burn.

The heteroplastic transplantation first method is that this is concerning equal to stand once big damage for the skin person, so method is seldom used with the skin for the skin person.And the benefit that this method is transplanted is of short duration, because the Grafted life-span has only fortnight at most.

It is almost not used to get this implantation method of fell from corpse.Because will in time obtain whole case histories of corpse and relevant corpse very big difficulty is arranged.Also exist morally simultaneously, jural difficulty and from the family members of the deceased's resistance.

This need make careful selection and the thorough risk of infection of getting rid of to supplying the skin person amnion.Amnion must be prepared by the professional of medical center, and must finish in 12 hours.As for its work-ing life,, be no more than a week also as the artificial skin surrogate.

Pigskin is in various animal skins, through repeatedly the probationary certificate pigskin is best as fell surrogate effect, because its anatomical features is similar to fell.But the anatomical features of this skin is just to have behind the anti-and preparation procedure through giving of necessity, and the work of careful selection animal and preparation skin is utmost point expense manpower and complexity very.

Less than five months piggys must be absolute health, the exhausted not damaged of skin.Piggy decaptitates after putting to death with electrocution, makes whole times of blood.A professional group works in the sterilization atmosphere the same with the medical center, and the first step all removes the skin of piggy to be made into thin slice with electrical equipment then down, and thin slice must cool off in the physiological serum that is housed in antibiotic, and envrionment temperature is up to 4 ℃.In a single day this skin be used for the life-span that the patient also had only for two weeks.

Artificial skin

People attempt to adopt sintetics to be used for the skin injury patient as skin substitute products for many years.Have two kinds of study routes to merit attention, first kind is that to seek all be synthetic, as polysiloxane, urethane and other as surrogate, second kind be consideration from animal blood, for example the derivative of collagen prepares organic materials and synthesizes film.

First kind all is that the skin of synthetic still is in conceptual phase, product-free still on the market.Second kind is artificial skin, is made by two integral layers.Manufacture with the exsiccant organic materials with one deck that wound directly contacts, preferably prepare from animal blood.Another layer is that polysiloxane, urethane etc. are as supporting layer, outside this one deck is exposed to.After organic moiety was organized absorption, supporting layer can be removed.This product is existing to be sold, and its characteristic has a detailed description in Brazilian patent P I7800285 number.The patentee is Battelle Memorial Institute.

The present invention is with surprising, and beat all method has solved the problems referred to above, the invention provides a kind of tunica fibrosa that different thickness is arranged.

The preparation tunica fibrosa is a biosynthetic process, promptly synthesizes by the breeding of bacterium.These bacteriums belong to Pseudomonadales, pseudomonadaceae, genus acetobacter, Xylinum kind.

The method that the present invention prepares tunica fibrosa comprises the steps:

Preparing a kind of is nutraceutical developing medium with nitrogen and carbohydrate; A kind of genus acetobacter of inoculation in this medium, the bacterium of Xylinum kind; In the time of the purposes of the expection that is fit to film, allowing to cultivate above-mentioned culture under the activated temperature condition of bacterium; At last the film that forms in the developing medium is taken out, when it is in swelling state, dewater, promptly make desired tunica fibrosa.Tunica fibrosa of the present invention can be used as the artificial leather implant piece, and separatory membrane is sewed up medium, the string of a musical instrument of tennis racket or leatheroid etc.

Tunica fibrosa of the present invention be translucent, have permselective property, feel fine, closely similar with fell.This film has semipermeability and elasticity, contacts very big clinging power with transudate in the injury.This film does not have above-mentioned various shortcoming, and compared with the prior art also has following advantage:

Film is inexpensive, is easy to preparation, can sterilize and never degenerates.

Appearance is given good opinion, helps the patient psychologically.

It is convenient to use, and does not need dressing.

Can be used for any position of human body, be easy to by its transudate, have enough elasticity to allow motion attached to injury region.

Be easy to storage, imputrescibility does not need freezing.

Can remove patient's misery immediately, because it has protected nerve ending.

Film has reduced the surge of bacterium to the strong adhesive force of injury, makes to hinder face and well sealed.

Water-dielectric loss reduces.Can shorten the time of scabbing to second degree burn, can shorten the time that forms granulation third degree burn.

Can not produce the scar of contraction.

Owing to do not need to change dressing, do not have infectious danger.

Do not have anaphylaxis.

Because film is translucent, the situation of the affected part development that can detect by an unaided eye.This is just in time opposite with other existing artificial skin.

Executing film must be very careful when damage location, in order to avoid bubble and blood system bleeding are clipped in film and hinder between the face.In case film is placed the transudate place, and covered all hinder face after, film just absorbs transudate gradually lentamente.Just, just provide the agglomerative condition, at film and hinder formation one bridge between the face to the transudate in film because this process is carried out slowly.Because absorption process is slow, before infiltrating in the film, transudate just condenses again.Condense and be created in two places:

A: in the micro-pore between membrane fiber;

B: the surface of injury.

So just produce an intact bonding, anti-sealing-dielectric medium runs off and significantly reduces the danger of infection.In addition, because nerve ending is isolated, can alleviate the misery of hindering face immediately.

So this film might make the patient recover epithelium, isolated because hinder face, can avoid again polluting.In the case, dressing need not changed, because film disappears very slow in tissue regeneration with the clinging power of hindering face.Film all is to be made of fiber, and fiber is an inert substance, does not have any medicine effect to hindering face.After providing such condition to make to hinder covering weave regeneration to organism, film stops to adhere to hindering face, does not stay resistates on second face.Film becomes characteristic the same when applying ointment or plaster, and loses viscosity, so to regard a kind of restorer or the temporary transient thing of implanting simply as.

Because tunica fibrosa intensity is big, and is inert, so can stand various sterilization methods, can preserve under any temperature, does not require special conditions.Be unconfined its work-ing life.Except that having other specific physical property, it has certain perviousness to liquid and air, and a specific molecular weight and a structure arranged, but the thickness of a prediction is arranged after dehydration.

For a definition more completely being arranged for skin-grafting material of the present invention, once done the research of following physics and spectroscopy.

To this, with the sample of film, part is bleached with 30% chlorine bleach liquor, with after stain with color.Stay a part keep original, without the situation of bleaching.The result who obtains is as follows:

Experimental film

1. unbleached to the bleaching of the penetration degree of air

The Bendtsen method, ml/min 11 8

(150WG)

2. to resistance>1800 of air second>1800 seconds

The Gurley method, second/100 milliliters

NBR 7152/82

(ABNT-MB-1271/79)

3. tensile strength kilograms per centimeter 2-7.92

NBR 7462/82

(ABNT-MB 57/68)

4. elongation %-74

NBR 7462/82

(ABNT-MB57/68)

Annotate: in order to carry out physical test, make the water content of film reach 25 ± 5% after, wire up as sample, during test will relative humidity be 65 ± 2% and temperature be to carry out under 20 ± 1 ℃ of conditions.Spectroscopic analysis shows no matter be bleaching or unbleached film, all to have the characteristic of fiber.

In spectral investigation, used following reference chart.This chart is taken from Rostislav Georgievich Zhbankov and is shown " infrared spectra of fiber and derivative thereof " book of publishing in 1966.

Reference table

Family/key compound wave number centimetre -1

Fiber and from 1400 to 1200

Derivative

Fiber and from 1400 to 1300

Derivative

Fiber and from 1200 to 1100

Derivative from 1150 to 1000

The preparation tunica fibrosa is a biosynthetic process, synthesizes by the breeding of bacterium.These bacteriums belong to Pseudomonadales, pseudomonadaceae, genus acetobacter, Xylinum kind.These microbionations generate a kind of fiber zoogloea and are enclosed in around the microorganism in the developing medium that carbohydrate nutritive substance and nitrogenous source are arranged.

This fiber zoogloea outer appearance cartilage, thickness changes between 0.2 to 400 millimeter.After sowing bacterial classification, a kind of vegetative propagation of schizogony has just begun.Cell is divided into two five equilibriums, and grows until forming a center ring, and bacterium is divided at this.For acetobacter, after the cell fission, new bacterium accumulates in around the indigenous bacteria, and begins its reproductive process immediately.In the ideal case, every interval just further divided once in 20 minutes.

Acetobacter is surrounded by fibrous mass, and they keep state of aggregation the division back, form chain.This and the associated characteristic of reproduction speed help bacterium chain and corresponding cenobium and couple together the formation tunica fibrosa.

The thickness of tunica fibrosa is variable, depends on various factors, such as the saturation point of nutrition dosage, temperature, time, bacterium colony and the type of developing medium.

The zoogloea of acetobacter is to be woven into by the bacterium chain that is surrounded by fibrous mass.Carrying out along with the zoogloea dehydration just can obtain pure tunica fibrosa.According to its characteristic, can do various industrial raw material, as be used for the surrogate of reparation, stitching medium or the leather of skin.

After the dehydration, the micro-pore that exists between membrane fiber is to make film possess the key of selective permeation characteristic.

The thickness of tunica fibrosa is all parameters, particularly:

A: the content of carbohydrate in the developing medium;

B: the content of nitrogen in the developing medium;

C: temperature;

D: zoogloea forms lasting.

Now illustrate the present invention for several different examples.Given example is nonrestrictive.

Example 1

20 gram Sinensis tea are soaked in the water of 10 liters and make developing medium, immersion liquid is filtered, add 1 kilogram of sugar in filtrate, mix then.

In developing medium, add 10 milliliters of genus acetobacters, the culture of Xylinum kind, and under 28 ℃, cultivated 36 hours, approximately thick 0.2 millimeter film has just formed.

Film is taken out from medium, it can be boiled according to the purposes of expection, the film that will be in swelling state then is placed on the support at ambient temperature and dewaters.The film that obtains so just can be prepared as artificial skin or artificial leather.The developing medium of separating from synthetic leather replenishes to remedy the loss that takes place in the biosynthetic process with original nutritive medium after filtering.

The concentration of carbohydrate is not really important in temperature of cultivating and the developing medium, can bacterium still can active very low-level and film still can be existed and indeformable high level between fluctuate.

Dehydration temperaturre is also not really important, and general thermal source all can use.

Example 2

Continue the process of example 1, allow nutrient solution cultivate after 96 hours and can obtain thick 3 millimeters zoogloea film, can be used as the artificial skin graft after this film dehydration.

Example 3

The method of use-case 1 still, but with the source of Paraquay tea as nitrogen.

Obtain the have same film that give dehydration thickness the same with example 1, incubation time is 72 hours.

Example 4

Take out film, spread on the damage zone that loses epithelium carefully, do not make film and hinder to form bubble or blood system bleeding between the face by above-mentioned all example preparations.In case film spreads on the transudate, and all cover and hinder face, the film absorption transudate, but can not allow the transudate loss, more can not advance air.

Like this, film just forms a new skin organizationally, owing to isolated nerve ending, has mechanically eliminated painful symptom.Through after a while, hinder face regeneration after, film breaks away from naturally hinders face, just can see an epithelium holomorphosis, new.

Tunica fibrosa of the present invention also can be used for surgical stapling, after the processing of necessity, a very strong ligation intensity can be arranged.

Film also can be used as the string of a musical instrument on diffusion barrier, dermatine, the tennis racket or other purposes of any common usable fibers.

Claims (10)

1, the method for a kind of tunica fibrosa of preparation is characterized in that comprising the steps:
Preparing a kind of is nutraceutical developing medium with nitrogen and carbohydrate;
A kind of genus acetobacter of inoculation in this medium, the bacterium of Xylinum kind;
In the time of the purposes of the expection that is fit to film, allowing to cultivate culture under the activated temperature condition of bacterium, and
The film that forms in the developing medium is taken out, and when it is in swelling state, dewater.
2, according to the method for claim 1, further comprise, in developing medium, replenish nutrition that loses and circulation developing medium.
According to the method for claim 1 or 2, it is characterized in that 3, nitrogen source is in Sinensis tea, carbohydrate derives from sucrose.
4, according to the method for claim 1, it is characterized in that at about 28 ℃, the cultivation through 26 to 96 hours makes film, thickness is 0.2 to 3 millimeter before its dehydration.
5, a kind of tunica fibrosa is characterized in that this tunica fibrosa is by either party's method preparation in the aforesaid right requirement.
6, a kind of artificial leather implant piece is characterized in that this artificial leather implant piece comprises by the dehydration tunica fibrosa of the method preparation of claim 1, then is cut into a certain size, sterilization and packing.
7, as the application of the tunica fibrosa of separatory membrane, this tunica fibrosa is by the method preparation of claim 1.
8, as the application of the tunica fibrosa of imitation leather, this tunica fibrosa is by the method preparation of claim 1.
9, be used to produce the application of the dehydration tunica fibrosa of the tennis racket or other the string of a musical instrument, this dehydration tunica fibrosa is by the method preparation of claim 1.
10, disclosed arbitrary new feature is used separately or is used in combination with new feature disclosed by the invention or further feature.
CN 85108100 1984-10-01 1985-09-30 Process for the preparation of cellulose film, cellulose film produced thereby, artificial skin graft and its use CN1015908B (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
BR8404937A BR8404937A (en) 1984-10-01 1984-10-01 Process for preparing cellulose film, cellulose film obtained by the same, artificial skin implant, injury treatment process using the referred cellulose film and use
BR8404937 1984-10-01

Publications (2)

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CN85108100A true CN85108100A (en) 1986-08-27
CN1015908B CN1015908B (en) 1992-03-18

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CN 85108100 CN1015908B (en) 1984-10-01 1985-09-30 Process for the preparation of cellulose film, cellulose film produced thereby, artificial skin graft and its use

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US (1) US4912049A (en)
EP (1) EP0197969B1 (en)
JP (1) JPH0636815B2 (en)
CN (1) CN1015908B (en)
BR (1) BR8404937A (en)
CA (1) CA1287006C (en)
DE (1) DE3577727D1 (en)
ES (1) ES8609459A1 (en)
MX (1) MX162625A (en)
PT (1) PT81228B (en)
WO (1) WO1986002095A1 (en)

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0200409B1 (en) * 1985-04-16 1994-06-29 Sony Corporation Moulded material comprising bacteria-produced cellulose
US5871978A (en) * 1985-10-18 1999-02-16 Monsanto Life Sciences Co Method of producing reticulated cellulose having type II crystalline cellulose
US5144021A (en) 1985-10-18 1992-09-01 Weyerhaeuser Company Reticulated cellulose and methods and microorganisms for the production thereof
US5821109A (en) * 1985-10-18 1998-10-13 Monsanto Life Sciences Co. Reticulated cellulose and methods and microorganisms for the production thereof
US4863565A (en) * 1985-10-18 1989-09-05 Weyerhaeuser Company Sheeted products formed from reticulated microbial cellulose
DE3783073T2 (en) * 1986-04-22 1993-04-29 Ajinomoto Kk Modified cellulose gel made by microorganisms and its complex with animal cells.
US5079162A (en) * 1986-08-28 1992-01-07 Weyerhaeuser Company Reticulated cellulose and methods and microorganisms for the production thereof
BR8800781A (en) * 1988-02-24 1989-09-19 Bio Fill Produtos Biotecnologi PROCESS FOR THE PREPARATION OF SUSPENSIONS OR Aqueous PULP OF CELLULOSE MICROFIBRILLES, SUSPENSION OR Aqueous PULP OF CELLULOSE MICROFIBRILLES AND USE
JP2853165B2 (en) * 1989-05-29 1999-02-03 味の素株式会社 Body tissue adhesion prevention film
EP0416470A3 (en) * 1989-09-04 1991-08-07 Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung E.V. Process for making cellulose membranes from bacteria produced cellulose
JP2762632B2 (en) * 1989-11-27 1998-06-04 味の素株式会社 Biological tissue substitute
US5196190A (en) * 1990-10-03 1993-03-23 Zenith Technology Corporation, Limited Synthetic skin substitutes
US5846213A (en) * 1997-06-16 1998-12-08 The University Of Western Ontario Cellulose membrane and method for manufacture thereof
DE10135675A1 (en) * 2001-07-21 2003-03-06 Skw Biogarde Gmbh & Co Kg Permeable film of cellulose containing hyaluronic acid, useful e.g. as wound dressing or skin replacement, allows gas exchange and is transparent
US6986963B2 (en) 2001-12-14 2006-01-17 Ut-Battelle Llc Metallization of bacterial cellulose for electrical and electronic device manufacture
US20030203013A1 (en) * 2002-04-26 2003-10-30 Xylos Corporation Microbial cellulose wound dressing for treating chronic wounds
US20050019380A1 (en) * 2002-04-26 2005-01-27 Xylos Corporation Microbial cellulose wound dressing for treating chronic wounds
US7390499B2 (en) * 2002-04-26 2008-06-24 Lohmann & Rauscher Gmbh Microbial cellulose wound dressing for treating chronic wounds
US7704523B2 (en) * 2002-04-26 2010-04-27 Lohmann & Rauscher Gmbh Microbial cellulose wound dressing for treating chronic wounds
BR0205047C1 (en) * 2002-11-21 2003-11-04 Ronaldo Da Rocha Loures Bueno Biosynthetic cellulose membrane coated stent graft
BR0205499A (en) * 2002-12-05 2004-08-03 Nelson Luiz Ferreira Levy Process for obtaining mat and cellulosic membrane, process for obtaining matted cellulosic that incorporates other materials, culture medium used, closed fermentation trays, drying equipment used, cellulose membrane obtained by such process and uses of said matting and membranes
US20060134758A1 (en) * 2002-12-05 2006-06-22 Levy Nelson L F Process for obtaining a ccellulosic wet sheet and a membrane, the equipment used to obtain the membrane and the membrane obtained
US8951551B2 (en) * 2005-08-31 2015-02-10 Board Of Regents, The University Of Texas System Multiribbon nanocellulose as a matrix for wound healing
US20070128243A1 (en) 2005-12-02 2007-06-07 Xylos Corporation Implantable microbial cellulose materials for various medical applications
US7709631B2 (en) 2006-03-13 2010-05-04 Xylos Corporation Oxidized microbial cellulose and use thereof
BRPI0601330A (en) * 2006-03-31 2007-12-04 Wellborn Participacoes Societa topical composition of biocellulose in gel form, spray aerosol, cream and or aqueous suspension for treatment of epithelial lesions
WO2007140573A1 (en) * 2006-04-24 2007-12-13 Axcelon Biopolymers Corporation Nanosilver coated bacterial cellulose
US20070286884A1 (en) * 2006-06-13 2007-12-13 Xylos Corporation Implantable microbial cellulose materials for hard tissue repair and regeneration
US20080044456A1 (en) * 2006-08-17 2008-02-21 Wellborn Participacoes Societarias S/C Ltda Cellulose membrane drying process
ITTO20060932A1 (en) * 2006-12-29 2008-06-30 Medestea Res & Production S P A A process for the production of a cellulose-based film, to be used for the coverage, repair, regeneration and healing of skin lesions and tissue and so 'obtained film.
US7968646B2 (en) 2007-08-22 2011-06-28 Washington State University Method of in situ bioproduction and composition of bacterial cellulose nanocomposites
EP2070557A1 (en) 2007-12-12 2009-06-17 Xylos Corporation Implantable microbial cellulose materials for hard tissue repair and regeneration
US20090209897A1 (en) * 2008-02-20 2009-08-20 Lotec, Inc. Dba Vesta Sciences, Inc. Photoactivated Antimicrobial Wound Dressing and Method Relating Thereto
US7832857B2 (en) * 2008-08-18 2010-11-16 Levinson Dennis J Microbial cellulose contact lens
US8679779B2 (en) 2008-11-07 2014-03-25 Sofradim Production Medical devices with definable porosity produced by bacterial polymer bio-synthesis
US9107978B2 (en) 2008-11-07 2015-08-18 Sofradim Production Template for bacterial cellulose implant processed within bioreactor
US20110262521A1 (en) 2008-11-07 2011-10-27 Sofradim Production Medical device including bacterial cellulose reinforced by resorbable or non resorbable reinforcing materials
AU2009312479A1 (en) 2008-11-07 2010-05-14 Sofradim Production Medical device including a bacterial cellulose sheet, perforated or microperforated as a mesh
US8962006B2 (en) 2008-11-07 2015-02-24 Sofradim Production Composite mesh including a 3D mesh and a non porous film of oxidized cellulose from bacterial cellulose origin
WO2010065908A2 (en) * 2008-12-05 2010-06-10 Catchmark Jeffrey M Degradable biomolecule compositions
US20100158985A1 (en) 2008-12-19 2010-06-24 Xylos Corporation Porous structures of microbial-derived cellulose for in vivo implantation
US20100159046A1 (en) * 2008-12-19 2010-06-24 Xylos Corporation Minimal tissue attachment implantable materials
WO2010135234A2 (en) * 2009-05-18 2010-11-25 Cornell University Bacterial cellulose based 'green' composites
US20110086236A1 (en) * 2009-10-13 2011-04-14 The Penn State Research Foundation Composites containing polypeptides attached to polysaccharides and molecules
TWI408232B (en) 2010-05-24 2013-09-11 Nympheas Internationalcorp Bacterial cellulose film, and use thereof
DE102010050311A1 (en) 2010-11-03 2012-05-03 Bsn Medical Gmbh Wound dressing with a biocellulose layer that adsorbs bacteria
US10202517B2 (en) 2013-07-26 2019-02-12 The Penn State Research Foundation Polymer compositions and coatings
EP3148598B1 (en) 2014-05-30 2018-11-07 Sofradim Production Implant comprising oxidized cellulose and method for preparing such an implant

Family Cites Families (4)

* Cited by examiner, † Cited by third party
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US4378431A (en) * 1980-09-02 1983-03-29 The University Of N.C. At Chapel Hill Production of a cellulose-synthetic polymer composite fiber
US4588400A (en) * 1982-12-16 1986-05-13 Johnson & Johnson Products, Inc. Liquid loaded pad for medical applications

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EP0197969B1 (en) 1990-05-16
JPS62500630A (en) 1987-03-19
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PT81228A (en) 1985-10-01
EP0197969A1 (en) 1986-10-22
BR8404937A (en) 1986-05-06
DE3577727D1 (en) 1990-06-21
CN1015908B (en) 1992-03-18
JPH0636815B2 (en) 1994-05-18
US4912049A (en) 1990-03-27
MX162625A (en) 1991-06-07
ES8609459A1 (en) 1986-09-01
PT81228B (en) 1987-09-30
ES547478A0 (en) 1986-09-01
WO1986002095A1 (en) 1986-04-10

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