CN2515678Y - Test paper strip for fast detecting drug residue of animal body and its product - Google Patents
Test paper strip for fast detecting drug residue of animal body and its product Download PDFInfo
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- CN2515678Y CN2515678Y CN 02228103 CN02228103U CN2515678Y CN 2515678 Y CN2515678 Y CN 2515678Y CN 02228103 CN02228103 CN 02228103 CN 02228103 U CN02228103 U CN 02228103U CN 2515678 Y CN2515678 Y CN 2515678Y
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Abstract
The utility model relates to drug residue detecting appliances, in particular to test strips I and II that are used to quickly detect the drug residue in the animal body and the product of the animal body, comprising a supporting layer and a reaction reagent carrier adsorption layer. The supporting layer is a no absorbing water thin film phonograph strip; the reaction reagent carrier adsorption layer is pasted on the supporting layer. The sample terminal is sequentially provided with a fiber layer, and a corresponding colloidal-gold antibody (Wi) of the drug to be detected or a colloidal-gold carrier protein (Xi) fiber layer coupled with the drug to be detected. A cellulose film and the end of a handle are water absorption material layers. The cellulose film is provided with a detection blot <I> that contains the carrier protein (Xi) coupled with the drug to be detected or a corresponding monoclonal antibody or a polyclonal antibody of the drug to be detected, a control blot <I> and a combined blot <II> that contain IgG antibody Y of the sheep or the rabbit anti-mouse or a anti-carrier protein (Xi). The test strip adopts a brownish red mark to show the detected result, which is imaging, visual, and correct with strong specificity and high sensitivity. Thus, the misjudgment of the false positive or the false negative does not occur easily. With simple and quick operation, the utility model can greatly reduce the labor intensity, can shorten the detecting time and can reduce the detecting cost. Therefore, the utility model is easy to be popularized and applied.
Description
(1) technical field: the utility model relates to a kind of medicament residue appliance, particularly relates to the residual quick test strip of a kind of animal body and products thereof Chinese traditional medicine.
(2) background technology: modern animal husbandry provides extremely abundant meat, fowl, milk, egg food for the mankind, and the medicament residue of the animal foods that is on the rise has constituted huge threat and harm to health and life, problem such as cancer, deformity, the resistance to the action of a drug, teenager's sex premature, person in middle and old age's angiocardiopathy and some food poisoning, often with animal foods in the abuse of microbiotic, hormone and other synthetic drugs and residual closely related.
In the animal diseases control process, use veterinary drug, medicated premix and in feed, add some drugs, all can cause medicine residual in animal product, and use residual its harm that forbidden drug caused maximum.Though country pays much attention to the detection and the monitoring work of residue of veterinary drug, successively formulated and issued the maximum residue limit(MRL) of 110 kinds of medicines in animal food, method for detecting residue and other technology item, but China is all still having very big gap with developed country to aspects such as the detection of veterinary drugs in food of animal product and monitoring, detection to feed and veterinary drug can only rest on (protein content in the conventional detection, the calcium phosphorus content, micronutrient levels), and in feed, adding medicine, or even the medicament residue that forbidden drug caused, but be difficult to obtain effective law support and lack monitoring means.Though European Union thinks that the medicament residue standard of China works out, detection means does not put in place, does not have concrete medicament residue Supervisory Surveillance Program and embodiment, is since nineteen ninety-six keeping always and is forbidding the animal products import to China.
(3) utility model content: the purpose of this utility model is: develop animal body and products thereof medicament residue quick detection test paper bar I and II.
The technical solution of the utility model is: the residual quick test strip I of a kind of animal body and products thereof Chinese traditional medicine, contain supporting layer, reaction reagent carrier absorption layer, supporting layer is the strip of foil that do not absorb water, reaction reagent carrier absorption layer is pasted on the supporting layer, adsorbed layer is followed successively by fibrage by the sample end, adsorb the corresponding golden labeling antibody of medicine to be checked (Wi) fibrage, the cellulose rete, handle end is an absorbent material layer, on the cellulose rete, have contain lotus root join medicine to be detected carrier protein Xi detection trace " | " and the contrast trace " | " that contains sheep or the anti-mouse IgG antibody Y of rabbit is arranged.
The supporting layer slip that do not absorb water can be used the hard plastic slip; or the cardboard slip that do not absorb water; fibrage useable glass cotton; absorbent material layer can be used thieving paper; the cellulose rete can be used nitrocellulose filter; gold labeling antibody fibrage can be with golden labeling antibody (Wi) glass wool; gold labeling antibody (Wi) is the monoclonal antibody (Mi) or the polyclonal antibody of colloid gold label; the carrier protein (Xi) that lotus root joins corresponding medicine to be checked is that medicine to be checked and carrier protein lotus root connect the compound that closes; carrier protein can be bovine serum albumin(BSA) (BSA) X1; or be the pure albumen of ovum gallinaceum (OVA) X2; or be ferritin X3; or be hemocyanin X4 etc.; at glass wool; be coated with the plastic cement diaphragm on gold labeling antibody glass wool and the absorbent paper layer; the diaphragm deflection glass wool one side about 0.5cm place corresponding with golden labeling antibody (Wi) intersection at glass wool prints the sample mark line, and the contrast trace on cellulose membrane is " ‖ " with the detection trace and arranges.
Be adsorbed with the corresponding golden labeling antibody of certain medicine to be measured (Wi) glass wool, contain detection trace " | " that lotus root joins corresponding pharmaceutical carrier albumen to be checked (Xi) and can be a kind of in the following medicine: penicillin gold labeling antibody (W1) glass wool, lotus root connection penicillin carrier protein (Xi) trace, streptomysin gold labeling antibody (W2) glass wool, lotus root connection streptomysin carrier protein solution (Xi) trace, terramycin gold labeling antibody (W3) glass wool, lotus root connection terramycin carrier protein (Xi) trace, neomycin gold labeling antibody (W4) glass wool, lotus root connection neomycin carrier protein (Xi) trace, erythromycin gold labeling antibody (W5) glass wool, lotus root connection erythromycin carrier protein (Xi) trace, tetracycline gold labeling antibody (W6) glass wool, lotus root connection tetracycline carrier protein (Xi) trace, aureomycin gold labeling antibody (W7) glass wool, lotus root joins golden mould carrier protein (Xi) trace, chloromycetin gold labeling antibody (W8) glass wool, lotus root connection chloromycetin carrier protein (Xi) trace, gentamicin gold labeling antibody (W9) glass wool, lotus root connection gentamicin carrier protein (Xi) trace, kanamycins gold labeling antibody (W10) glass wool, lotus root connection kanamycins carrier protein (Xi) trace, Norfloxacin gold labeling antibody (W11) glass wool, lotus root connection Norfloxacin carrier protein (Xi) trace, Ofloxacin gold labeling antibody (W12) glass wool, lotus root connection Ofloxacin carrier protein (Xi) trace, Ciprofloxacin gold labeling antibody (W13) glass wool, lotus root connection Ciprofloxacin carrier protein trace, bacitracin gold labeling antibody (W14) glass wool, lotus root connection bacitracin carrier protein (Xi) trace, estrogen gold labeling antibody (W15) glass wool, lotus root connection estrogen carrier protein (Xi) trace, steroid hormone gold labeling antibody (W16) glass wool, lotus root connection steroid hormone carrier protein (Xi) trace, sulphadiazine gold labeling antibody (W17) glass wool, lotus root connection sulphadiazine carrier protein (Xi) trace, bacteresulf gold labeling antibody (W18) glass wool, lotus root connection bacteresulf carrier protein (Xi) trace, nitroimidazole gold labeling antibody (W19) glass wool, lotus root connection nitroimidazole carrier protein (Xi) trace, nitrofuran gold labeling antibody (W20) glass wool, lotus root connection nitrofuran carrier protein (Xi) solution trace, gram ball flour gold labeling antibody (W21) glass wool, lotus root connection gram ball powder carrier albumen (Xi) trace, aflatoxin gold labeling antibody (W22) glass wool, lotus root connection aflatoxin carrier protein (Xi) trace, FT gold labeling antibody (W23) glass wool, lotus root connection FT carrier protein (Xi) trace.
The residual quick test strip II of a kind of animal body and products thereof Chinese traditional medicine, contain supporting layer, reaction reagent carrier absorption layer, supporting layer is fixed on the supporting layer for the strip of foil that do not absorb water, reaction reagent carrier absorption layer, adsorbed layer is followed successively by fibrage by the sample end, the absorption lotus root joins gold mark carrier protein (Xi) fibrage of medicine to be checked, the cellulose rete, the handle end absorbent material layer contains the contrast trace " | " that drug antibody to be detected (Wi) detects trace " | " and contains anti-carrier protein Xi antibody on the cellulose rete.
The supporting layer slip that do not absorb water can be the hard plastic slip; or the cardboard slip that does not absorb water; fibrage can be glass wool; absorbent material layer can be used thieving paper; the cellulose rete can be used nitrocellulose filter; gold mark fibrage can join gold mark carrier protein (Xi) glass wool of medicine to be checked with lotus root; lotus root joins the gold of medicine to be checked and marks carrier protein (Xi) joins medicine to be checked for the lotus root of colloid gold label carrier protein; carrier protein (Xi) can be bovine serum albumin(BSA) (BSA) X1; or be (OVA) X2 of the pure albumen of ovum gallinaceum; or be ferritin X3; or be hemocyanin X4 etc.; at glass wool; carry out on gold mark carrier protein (Xi) glass wool and the water accepting layer and be stamped diaphragm; the about 0.5cm of diaphragm deflection glass wool one side place in glass wool and gold mark carrier glass wool intersection correspondence prints the sample mark line, and the combination of detection trace on the cellulose rete and contrast trace is arranged and be can be " ‖ ".
Be adsorbed with lotus root and join the gold mark carrier protein Xi glass wool of medicine to be checked, medicine corresponding antibody to be detected (Wi) detects trace " | ", can be a kind of in the following medicine: lotus root connection penicillin gold mark carrier protein Xi glass wool, penicillin antibody W1 trace, lotus root connection streptomysin gold mark carrier protein Xi glass wool, streptomysin antibody W2 trace, lotus root connection terramycin gold mark carrier protein Xi glass wool, terramycin antibody W3 trace, lotus root connection neomycin gold mark carrier protein Xi glass wool, neomycin antibody W4 trace, lotus root connection erythromycin gold mark carrier protein Xi glass wool, erythromycin antibody W5 trace, lotus root connection tetracycline gold mark carrier protein Xi glass wool, tetracycline antibody W6 trace, lotus root connection aureomycin gold mark carrier protein Xi glass wool, chlortetracycline antibody W7 trace, lotus root connection chloromycetin gold mark carrier protein Xi glass wool, chloramphenicol antibody W8 trace, lotus root connection gentamicin gold mark carrier protein Xi glass wool, gentamicin antibody W9 trace, lotus root connection kanamycins gold mark carrier protein Xi glass wool, kanamycins antibody W10 trace, lotus root connection Norfloxacin gold mark carrier protein Xi glass wool, Norfloxacin antibody W11 trace, lotus root connection Ofloxacin gold mark carrier protein Xi glass wool, Ofloxacin antibody W12 trace, lotus root connection Ciprofloxacin gold mark carrier protein Xi glass wool, Ciprofloxacin antibody W13 trace, lotus root connection bacitracin gold mark carrier protein Xi glass wool, bacitracin antibody W14 trace, lotus root connection estrogen gold mark carrier protein Xi glass wool, estrogen antibody W15 trace, lotus root connection steroid hormone gold mark carrier protein Xi glass wool, steroid hormone antibody W16 trace, lotus root connection sulphadiazine gold mark carrier protein Xi glass wool, sulphadiazine antibody W17 trace, lotus root connection bacteresulf gold mark carrier protein Xi glass wool, bacteresulf antibody W18 trace, lotus root connection nitroimidazole gold mark carrier protein Xi glass wool, nitroimidazole antibody W19 trace, lotus root connection nitrofuran gold mark carrier protein Xi glass wool, nitrofuran antibody W20 trace, lotus root connection gram ball flour gold mark carrier protein Xi glass wool, gram ball powder antibody W21 trace, lotus root connection aflatoxin gold mark carrier protein Xi glass wool, aflatoxin antibody W22 trace, lotus root connection FT gold mark carrier protein Xi glass wool, FT antibody W23 trace.
The good effect that the utility model is useful:
1. the residual quick test strip of animal body and products thereof Chinese traditional medicine is widely used in the residual fast detecting of following various kinds of drug.
Sulfamido, itrofurans, nitro glyoxaline, macrolides, quinoxaline class, Tetracyclines, aminoglycosides and beta-lactam; As penicillin, streptomysin, terramycin, neomycin, erythromycin, tetracycline, chloromycetin, gentamicin, kanamycins, Norfloxacin, Ofloxacin, Ciprofloxacin, bacitracin, estrogen, steroid hormone, sulphadiazine, bacteresulf, nitroimidazole, nitrofuran, gram ball powder, aflatoxin, FT.
The kind that causes the medicine that the animal product Chinese traditional medicine is residual and be detrimental to health mainly contains:
Forbidden drug and the medicine that does not go through to use, as: hormone, thyroid imhibitor, the pure and mild calm class medicine of Gibberella zeae etc.;
The anti-microbial type medicine that people and animals are shared is as sulfamido, itrofurans, nitrofuran azole, macrolides, quinoxaline class, Tetracyclines, aminoglycosides and beta-lactam; The types of drugs that other uses such as permission, but fail to observe the regulation of off-drug period, as penicillin, chloromycetin, kanamycins, the big mould rope of celebrating, QNS.
2, medicament residue quick detection test paper bar has following advantage
(1) high specificity, susceptibility height.The quick detection test paper bar is that the basis is prepared from the monoclonal antibody (Mi) of colloid gold label high-affinity, no covalent bond formation between gold grain and antibody molecule in the gold labeling antibody, the two combines by the Van der Waals force between the charges of different polarity, colloid gold label is very little to the specificity and the affinity influence of monoclonal antibody (Mi), and has higher mark rate.Therefore, the quick detection test paper bar has higher specificity and susceptibility.Susceptibility is as the criterion with the index that The Ministry of Agriculture of the People's Republic of China, MOA formulates, be that the notice about issue " animal food herbal medicine maximum residue limit(MRL) " is sent out in agriculture and animal husbandry [1999] No. 17, detect minimum limiting the quantity of and be the maximum residue limit(MRL) of notification, promptly be higher than maximum residue limit(MRL) or equal maximum residue limit(MRL) and all can detect, then can not detect when being lower than maximum residue limit(MRL).
(2) easy and simple to handle quick.Need not any other reagent when using the quick detection test paper bar,, in 2 minutes, get final product result of determination as long as be inserted into detected sample about 10 seconds.
(3) show testing result image, accurately directly perceived.The quick detection test paper bar is to show that rufous " | " and " ‖ " trace are as the positive and the negative marker that detect, showing on cellulose membrane promptly that a brownish red " | " trace is illustrated in detects medicine to be checked and exceeds standard residual in the sample, article two, brownish red " ‖ " trace is illustrated in and does not detect medicine to be checked in the sample and exceed standard residual, the result judges image, directly perceived, accurate, simple and clear, be not prone to the erroneous judgement of false negative and false positive.
(4) cost is low, small investment.Use the quick detection test paper bar, do not need to join in addition instrument and equipment and other reagent, the on-the-spot detection settled at one go, with low cost, small investment, instant effect.
(5) be easy to apply.The quick detection test paper bar is easy and simple to handle, need not to train in advance, and the people can operate per capita, and can carry out the scene and detect, and is fit to China's quarantine, monitoring national conditions.
The quick detection test paper bar is widely used in being engaged in professional chemical examination, customs quarantine control, health and epidemic prevention, quality monitoring, livestock products processing, the intensive culture demand to each levels such as individual breed, has vast market prospect and bigger economical, societal benefits.
(4) description of drawings:
Fig. 1: the residual quick test strip of animal body and products thereof Chinese traditional medicine (I) structural representation;
Fig. 2: the residual quick test strip of animal body and products thereof Chinese traditional medicine (I) plan structure synoptic diagram;
Fig. 3: the residual quick test strip of animal body and products thereof Chinese traditional medicine (II) structural representation;
Fig. 4: the residual quick test strip of animal body and products thereof Chinese traditional medicine (II) plan structure synoptic diagram;
(5) specific embodiment:
The residual quick test strip of animal body and products thereof Chinese traditional medicine can be widely used in the fast detecting of multiple left drug, microbiotic, hormone etc., as penicillin, streptomysin, terramycin, erythromycin, tetracycline, chloromycetin, gentamicin, kanamycins, Norfloxacin, Ofloxacin, Ciprofloxacin, bacitracin, estrogen, steroid hormone, sulphadiazine, bacteresulf, nitroimidazole, nitrofuran, gram ball powder, aflatoxin, FT etc.
Certain medicament residue quick detection test paper bar in preparation animal body and products thereof, at first need prepare the carrier protein that lotus root joins this kind medicine, be used for printing the detection trace, and then the golden labeling antibody of corresponding this kind medicine of preparation, be used to prepare the golden labeling antibody fiber of this kind medicine, secondly need preparation sheep or the anti-mouse IgG antibody of rabbit, be used for printing the contrast trace.
(1) lotus root joins the preparation of the carrier protein (Xi) of certain medicine
Adopt carbodiimide method, diazotising method or glutaraldehyde method, corresponding medicine to be checked and carrier protein are carried out lotus root connection preparation immunizing antigen.The 5mg medicine is dissolved in the PBS solution of precooling, add carrier protein then, add than the lotus root connection medicine that adds 1 molecule for per 50 amino acid of carrier protein, place and stir on the vent cabinet magnetic stirring apparatus, slowly add isopyknic 0.2% glutaraldehyde PBS solution, stirring at room 1 hour, the glycocoll PBS solution final concentration that adds 1M is 200mM, continues to stir 1 hour, with PBS liquid dialysed overnight, change liquid 4 times, remove and unload this albumen and unnecessary medicine.
(2) preparation of anti-certain anti-drug monoclonal antibody (Mi)
Joining certain pharmaceutical carrier protein immunization BALB/c with 50 μ g-100 μ g/ lotus root only is mouse three times, each 15-30 days at interval; Behind the booster immunization 3-4 days for the third time, with the bloodletting of immune mouse eyeball, draw neck to cause death, in 75% alcohol-pickled 5-10min, aseptic its spleen of getting shreds and through 100 order nylon net filters, the centrifugal 10min of 1000r/min collects splenocyte; With 1 * 10
8Splenocyte and 2-5 * 10
7NSO plasmacytoma mixing with cells, 1000r/min is centrifugal, and 10min abandons supernatant, cell precipitation slowly adds 40%-50%PEG4000 (pH8.5-9.0) the effect 1min of 0.7-1ml in 37 ℃ of water-baths, slowly add serum-free 1640 nutrient culture media 15ml then, to stop the effect of PEG, 37 ℃ of water-bath 5-10min, 1000r/min is centrifugal, and 10min abandons supernatant, cell precipitation is resuspended in HAT selects in the nutrient culture media, and add 96 well culture plates (100 μ l-200 μ l/ hole), put 37 ℃ of 5%CO
2Cultivate in the incubator.Cultivate after 7-10 days, join this kind pharmaceutical carrier albumen coated elisa plate (40 holes/piece), detect the culture supernatant of hybridoma, picking strong positive cell clone (OD with enzyme immunosorbent adsorption test (ELISA) with the lotus root of 5 μ g-10 μ g/ml
492More than=0.8), carry out continuous three times limiting dilution assay cloning, the hybridoma chromosome number that is produced is 92-98, the monoclonal antibody of its secretion (Mi) specifically with this kind drug response, and not with other irrelevant albumen generation cross reaction, affinity constant reaches 10
9-10, light chain subtype is κ or λ, the heavy chain hypotype is IgG
1, IgG
2a, IgG
2bOr IgG
3, the monoclonal antibody (Mi) at this kind medicine specific antigen determinant is used to prepare golden labeling antibody cotton.Above-mentioned preparation method is applicable to the preparation of any anti-drug monoclonal antibody (Mi).
(3) certain medicine gold labeling antibody (Wi) or lotus root join the preparation of gold mark carrier protein (Xi) glass wool of medicine to be checked
Prepare aurosol with the sodium citrate reducing process, promptly in the 50-100ml0.01%-0.05% aqueous solution of chloraurate of boiling, add 0.5%～2% citric acid three sodium solution of 2-4ml, obtain the collaurum about diameter 15nm.With 0.1mol/LK
2CO
3Transfer collaurum pH to 8.5-9.5, add in the pH8.5-9.5 aurosol than monoclonal antibody (Mi) with 1: 1000～1: 3000 mark certain medicine to be marked, behind the mark 10min, add 20%PEG10000 to final concentration 0.05%, 4 ℃ of centrifugal 20imn of 500-3000g remove unconjugated gold grain.4 ℃ of centrifugal 1h of 15000g abandon supernatant, obtain preliminary purification gold mark protein mixture after, with propylene glucosan S-400 column chromatography, separation and purification gold mark protein obtains certain medicine colloid gold label antibody.The glue gold labelled antibody of dilution in 1: 50～1: 400 is adsorbed in the processed glass cotton, 4 ℃ of low-temperature vacuum dryings, prepare certain medicine gold labeling antibody cotton, this preparation method is applicable to the preparation of preparation of any medicine gold labeling antibody cotton and gold mark carrier protein (Xi) glass wool that lotus root joins medicine to be checked.
(4) preparation of anti-mouse IgG antibody of sheep or rabbit or anti-carrier protein (Xi) antibody
Extract mice serum IgG albumen with saturated ammonium sulfate, get 1 part of mice serum and add 2 parts of PBS (7.2) mixing, add equal-volume saturated ammonium sulfate mixing, put 4 ℃ of refrigerator 2h, 4 ℃ of centrifugal 15min of 12000r/min abandon supernatant; With an amount of PBS (7.2) dissolution precipitation, add saturated ammonium sulfate to final concentration 33%, put 4 ℃ of refrigerator 2h, 4 ℃ of centrifugal 15min of 12000/rmin, abandon supernatant, with a small amount of PBS (7.2) dissolution precipitation, put 4 ℃ of refrigerators dialyzed overnight in PBS (7.2), change liquid 2-3 time, 4 ℃ of centrifugal 15min of 12000r/min15min, collect supernatant, measure its protein concentration with ultraviolet spectrophotometer, through subcutaneous and intramuscular injection immune health sheep or 3-4 last of rabbit after immune 10 days, venous blood collection is measured its serum antibody titer more than 1: 2000 with ELISA with the mice serum IgG albumen of 50 μ g-100 μ g/kg body weight, heart blood sampling or arteria carotis bloodletting, collect its hyper-immune serum, extract sheep or the anti-mouse IgG of rabbit and anti-carrier protein (Xi) antibody (method is identical with extraction mice serum IgGSH, does not repeat) with saturated ammonium sulfate and be used for the preparation that any medicament residue quick detection test paper bar contrast shows trace.
(5) medicament residue quick detection test paper bar is implemented structure
Referring to Fig. 1,2, among the figure 1 for supporting layer with the strip of foil that do not absorb water, can adopt the plastic slice bar in the enforcement or adopt the hard sheet that does not absorb water, reaction reagent carrier absorption layer is by 2,3,4,5, combine, from sample end 2, above handle end 5 sticks on supporting layer 1 successively, wherein 2 is sample end fibrage, can use glass fibre to be called for short glass wool in the enforcement, 3 for being adsorbed with certain medicine gold labeling antibody (Wi) fibrage to be checked or adsorbing gold mark carrier protein (Xi) glass wool that lotus root joins medicine to be checked, can adopt the processed glass cellucotton, abbreviate golden labeling antibody cotton as, 4 is the cellulose rete, can adopt nitrocellulose filter in the enforcement, 5 is the handle end absorbent material layer, adopt thieving paper, all can as filter paper or other thieving paper, test strips length overall 8cm, width 0.4cm, 6 for to join certain pharmaceutical carrier albumen (Xi) solution or drug antibody solution to be checked trace " | " on cellulose membrane with lotus root, 7 contrast traces " | " for printing on cellulose membrane with sheep or the anti-mouse IgG antibody of rabbit (Y) solution or anti-carrier protein (Xi) antibody-solutions, the combination trace is " ‖ ".
8 is diaphragm, and the diaphragm that covers glass wool and golden labeling antibody (Wi) glass wool is 8-1, and the diaphragm that covers on the water accepting layer filter paper is 8-2.Be printed on a mark line 9 on the deflection glass wool one side 0.5cm place diaphragm on the white diaphragm of glass wool and golden labeling antibody (Wi) glass wool intersection correspondence position; be printed on arrow and max printed words on 9 lines the right; the handle end diaphragm can be with yellow or other color, the intersection fiber infiltration that crosses one another each other of 2,3,4,5 each layers.
(6) medicament residue quick detection test paper bar is implemented the detection reaction principle
When gold mark fibrage adsorbate is the corresponding golden labeling antibody Wi of medicine to be checked, after then medicament residue quick detection test paper bar (I) sample end inserts detected sample solution, solution to be checked spreads to nitrocellulose filter together by the golden labeling antibody (Wi) that siphon drives in certain medicine to be checked and the golden labeling antibody cotton, and finally infiltrate in the filter paper, certain medicine to be checked can combine with the golden labeling antibody (Wi) of this kind medicine in diffusion process, and then seal the antigen binding site that golden labeling antibody (Wi) is gone up this kind medicine, the detection trace that stops lotus root on golden labeling antibody (Wi) and the cellulose membrane to join this kind pharmaceutical carrier albumen (Xi) combines, can not show that rufous detects trace, the anti-mouse IgG antibody of sheep or rabbit (Y) then can combine with golden labeling antibody (Wi), form rufous contrast trace " | ", the i.e. positive mark of a rufous " | " trace, otherwise there is not certain medicine to be checked in the sample solution, then can not stop on golden labeling antibody " Wi " and the cellulose membrane lotus root to join this kind pharmaceutical carrier albumen " Xi " detects trace and combines, show that rufous detects trace " | ", sheep or the anti-mouse IgG antibody of rabbit (Y) also combine with golden labeling antibody (Wi) simultaneously, show rufous contrast trace " | ", form two rufous " ‖ " negative marker.When gold mark fibrage adsorbate is that lotus root is when joining the gold mark carrier protein (Xi) of medicine to be checked, after then medicament residue quick detection test paper bar (II) sample end inserts detected sample solution, solution to be checked drives the gold mark carrier protein (Xi) that certain medicine to be checked and lotus root join medicine to be checked by siphon and spreads to nitrocellulose membrane together, medicine to be checked in the sample and lotus root connection carrier protein medicine to be checked is competitive to be combined with the detection trace of medicine corresponding antibodies to be checked (Wi) on the cellulose membrane, stop the detection trace of medicine corresponding antibodies to be checked (Wi) on lotus root connection carrier protein medicine to be checked and the tunica fibrosa to combine, can not show that rufous detects trace " | ", and lotus root join medicine to be checked gold mark carrier protein (xi) can with the antibodies of anti-carrier protein, show that rufous detects trace " | ", the i.e. positive mark of a rufous " | " trace, otherwise there is not certain medicine to be checked in the sample solution, then can not stop the detection trace of medicine corresponding antibodies to be checked (Wi) on lotus root connection medicine and the tunica fibrosa to combine, show that rufous detects trace " | ", same lotus root join medicine to be checked gold mark carrier protein (Xi) can with the antibodies of anti-carrier protein, show rufous contrast trace " | ", form two rufous " ‖ " negative marker.If do not have the rufous mark to show on the cellulose membrane, show that then test strips lost efficacy.In order to reduce the susceptibility of test strips, improve and detect minimum numerical value of limiting the quantity of, make its limit of identification meet the maximum residue limit(MRL) of Ministry of Agriculture's regulation, therefore when the golden labeling antibody of preparation test strips is cotton, reduce the dilution ratio of golden labeling antibody, by reduce to 1: 50 at 1: 500～1: 400, because the increase of golden labeling antibody, could seal golden labeling antibody when then needing to be higher than the medicine of national maximum residue limit(MRL), stop golden labeling antibody (Wi) to combine with the detection trace, do not show the detection trace, be indicated as the positive, can not eat; When drug residue is lower than the index of national regulation, then the medicine in the sample can not seal golden labeling antibody, and golden labeling antibody still can combine with the detection trace that the cellulose membrane lotus root joins this kind pharmaceutical carrier albumen (Xi), then shows and detects trace, be indicated as feminine gender, edible.Another method is the dilution test sample, measures the content that converses the sample Chinese traditional medicine behind the result, determines whether edible then.Along with the change of national standard, can change the dilution ratio of golden labeling antibody, thereby improve susceptibility.
(7) medicament residue quick detection test paper bar embodiment detection method
The preparation of test sample liquid, detecting meat and goods can shred sample, grind, and makes 1 with physiological saline: 2-1: 10 sample suspension to be checked, egg and milk can directly make 1 with physiological saline: 2-1: 10 detected sample suspensions.
Corresponding medicament residue quick detection test paper bar sample end is inserted sample solution to be checked, and insertion depth does not surpass mark line 9, takes out test strip after about 5 seconds, about 2 minutes of horizontal positioned, observations.
The result judges: if there is a rufous mark " | " to show on cellulose membrane, the expression testing result is positive, promptly in institute's sample product, detect certain medicament residue, if there are two rufous marks " ‖ " to show on the cellulose membrane, the expression testing result is negative, promptly in institute's sample product, do not detect certain medicine,, show that then test strips lost efficacy if there is not the rufous mark to show on the cellulose membrane.
Embodiment one and two, the residual quick test paper bar of animals and animal product penicillin (I) and (II), referring to Fig. 1,2,3,4, carrier protein (X1) solution that joins penicillin earlier with reference to the foregoing description (1) preparation lotus root, by (2) preparation penicillin monoclonal antibody W1, by the gold mark carrier protein (X1) of (3) preparation penicillin gold labeling antibody W1 and golden labeling antibody (W1) cotton or lotus root connection penicillin and gold mark carrier protein (Xi) glass wool of lotus root connection penicillin, by (4) preparation sheep or anti-mouse IgG antibody of rabbit or carrier protein antibody, the residual quick test strip of penicillin (I) and (II) in the animals and animal product, referring to Fig. 1,2,3,4,1 is supporting layer among the figure, adopt the hard slip that does not absorb water the plastic slice bar or do not absorb water, reaction reagent carrier absorption layer is by 2,3-1,4,5 form, stick on the supporting layer 1, begin to be the microglass fiber layer from sample end 2,3-1 is that penicillin gold labeling antibody W1 microglass fiber layer or 3-2 are gold mark carrier protein (Xi) the microglass fiber layer of lotus root connection penicillin, i.e. gold mark glass wool, 4 is the cellulose rete, adopt nitrocellulose filter, 5 is the handle end absorbent material layer, adopts absorbent filter, test strips always is about 8cm, wide about 0.4cm, the detection trace " | " of 6-1 on the cellulose rete, printing with lotus root connection penicillin carrier protein (Xi) solution or 6-2 penicillin antibody W1 solution, 7-1 is for being the contrast trace " | " that anti-carrier protein antibody (Xi) is printed on cellulose membrane with sheep or the anti-mouse IgG antibody of rabbit (Y) or 7-2.
8-1 is the white diaphragm on the cotton and golden mark glass wool of cover glass; 8-2 is the yellow diaphragm that covers on the water accepting layer filter paper; on the diaphragm 8-1 at glass wool 2 and the about 0.5cm of gold mark glass wool intersection correspondence position deflection glass wool 2 one sides place, be printed on a mark line 9; online 9 the right are printed on arrow and max printed words; 2; 3-1 or 3-2,4,5 each layers intersection infiltration that should cross one another each other.
Detect by (7) method step among the embodiment, preparation solution to be detected as detecting beef, shreds beef earlier earlier, grinds, and makes 1: 2～1: 10 beef sample suspension to be checked with physiological saline.With the residual quick test strip of the penicillin in animal body and products thereof, the sample end inserts in the beef sample suspension to be checked, and insertion depth does not surpass mark line 9, takes out test strip after about 5 seconds, about 2 minutes observationss of horizontal positioned.
The result judges: if there is a rufous mark " | " to show on the plain film of test strips, the expression testing result is positive promptly in institute's test sample, penicillin is residual to exceed standard, if there are two rufous marks " ‖ " to show on the cellulose rete, the expression testing result is negative, in the expression institute test sample, penicillin is residual not to exceed standard, if there is not the rufous mark to show on the cellulose membrane, show that then test strips lost efficacy, can not use.
Embodiment three and four, animal body and products thereof streptomysin quick detection test paper bar (I) and (II), referring to Fig. 1,2,3,4, the structure of present embodiment test strips, method for making detects and the result observes and shows with embodiment one and two similarly, does not repeat in detail.Somewhat differently be: 3-1 is that streptomysin gold labeling antibody (W2) or 3-2 are the gold mark carrier protein of lotus root connection streptomysin, and 6-1 is that the carrier protein (Xi) of lotus root connection streptomysin or the antibody (W2) that 3-2 is anti-streptomycin detect trace " | ".
Embodiment five and six, animal body and products thereof terramycin quick detection test paper bar (I) and (II).Roughly the same embodiment one and two does not repeat.What some was different is: 3-1 is that terramycin gold labeling antibody (W3) or 3-2 are the gold mark carrier protein (Xi) of lotus root connection terramycin, and 6-1 is that the carrier protein (Xi) of lotus root connection terramycin or the antibody (W3) that 6-2 is anti-terramycin detect trace " | ".
Embodiment seven and eight, animal body and products thereof neomycin quick detection test paper bar (I) and (II).Roughly the same embodiment one and two does not repeat.What some was different is: 3-1 is that neomycin gold labeling antibody (W4) or 3-2 are the gold mark carrier protein (Xi) of lotus root connection neomycin, and 6-1 is that the carrier protein (Xi) of lotus root connection neomycin or the antibody (W4) that 6-2 is antimycin detect trace " | ".
Embodiment nine and ten, animal body and products thereof erythromycin quick detection test paper bar (I) and (II).Roughly the same embodiment one and two does not repeat.What some was different is: 3-1 is that erythromycin gold labeling antibody (W5) or 3-2 are the gold mark carrier protein (Xi) of lotus root connection erythromycin, and 6-1 is that the carrier protein (Xi) of lotus root connection erythromycin or the antibody (W5) that 6-2 is anti-erythromycin detect trace " | ".
Embodiment 11 and 12, animal body and products thereof tetracycline quick detection test paper bar (I) and (II).Roughly the same embodiment one and two does not repeat.Some is not both: 3-1 is that tetracycline gold labeling antibody (W6) or 3-2 are the gold mark carrier protein (Xi) of lotus root connection tetracycline, and 6-1 is that lotus root connection tetracycline carrier protein (Xi) or 6-2 are antibody (W6) the detection trace " | " of tetracycline resistance.
Embodiment 13 and 14, animal body and products thereof aureomycin quick detection test paper bar (I) and (II).Roughly the same embodiment one and two does not repeat.What some was different is: 3-1 is that aureomycin gold labeling antibody (W7) or 3-2 are the aureomycin gold mark carrier protein (Xi) of lotus root connection, and 6-1 is that the carrier protein (Xi) of lotus root connection aureomycin or the antibody (W7) that 6-2 is anti-aureomycin detect trace " | ".
Embodiment 15 and 16, animal body and products thereof chlorine is mould to be quick detection test paper bar (I) and (II).Roughly the same embodiment one and two does not repeat.What some was different is: 3-1 is that chloromycetin gold labeling antibody (W8) or 3-2 are the gold mark carrier protein (Xi) of lotus root connection chloromycetin, and 6-1 is that the carrier body protein (Xi) of lotus root connection chloromycetin or the antibody (W8) that 6-2 is chloramphenicol resistance detect trace " | ".
Embodiment 17 and 18, animal body and products thereof celebrating big mould quick detection test paper bar (I) and (II).Roughly the same embodiment one and two does not repeat.What some was different is: 3-1 is that gentamicin gold labeling antibody (W9) or 3-2 are the gold mark carrier protein (Xi) of lotus root connection gentamicin, and 6-1 is that the carrier protein (Xi) of lotus root connection gentamicin or the antibody (W9) that 6-2 is anti-gentamicin detect trace " | ".
Embodiment 19 and 20, animal body and products thereof kanamycins quick detection test paper bar (I) and (II).Roughly the same embodiment one and two does not repeat.What some was different is: 3-1 is that kanamycins gold labeling antibody (W10) or 3-2 are the gold mark carrier protein (Xi) of lotus root connection kanamycins, and 6-1 is that the carrier protein (Xi) of lotus root connection kanamycins or the antibody (W10) that 6-2 is anti-kanamycins detect trace " | ".
Embodiment 21 and 22, animal body and products thereof Norfloxacin quick detection test paper bar (I) and (II).Roughly the same embodiment one and two does not repeat.What some was different is: 3-1 is that Norfloxacin gold labeling antibody (W11) or 3-2 are the gold mark carrier protein (Xi) of lotus root connection Norfloxacin, and 6-1 is that the carrier protein (Xi) of lotus root connection Norfloxacin or the antibody (W11) that 6-2 is anti-norfloxacin detect trace " | ".
Embodiment 23 and 24, animal body and products thereof Ofloxacin quick detection test paper bar (I) and (II).Roughly the same embodiment one and two does not repeat.What some was different is: 3-1 is that Ofloxacin gold labeling antibody (W12) or 3-2 are the gold mark carrier protein (Xi) of lotus root connection Ofloxacin, and 6-1 is that the carrier protein (Xi) of lotus root connection Ofloxacin or the antibody (W12) that 6-2 is the antioxygen Flucloxacillin detect trace " | ".
Embodiment 25 and 26, animal body and products thereof Ciprofloxacin quick detection test paper bar (I) and (II), roughly the same embodiment one and two does not repeat.What some was different is: 3-1 is that Ciprofloxacin gold labeling antibody (W13) or 3-2 are the gold mark carrier protein (Xi) of lotus root connection Ciprofloxacin, and 6-1 is that the carrier protein (Xi) of lotus root connection Ciprofloxacin or the antibody (W13) that 6-2 is anti-Ciprofloxacin detect trace " | ".
Embodiment 27 and 28, animal body and products thereof bacitracin quick detection test paper bar (I) and (II).Roughly the same embodiment one, do not repeat.What some was different is: 3-1 is that bacitracin gold labeling antibody (W14) or 3-2 are the gold mark carrier protein (Xi) of lotus root connection bacitracin, and 6-1 is that the carrier protein (Xi) of lotus root connection bacitracin or the antibody (W14) that 6-2 is anti-bacitracin detect trace " | ".
Embodiment 29 and 30, animal body and products thereof estrogen quick detection test paper bar (I) and (II).Roughly the same embodiment one and two does not repeat.What some was different is: 3-1 is that estrogen gold labeling antibody (W15) or 3-2 are the estrogenic gold mark of lotus root connection carrier protein (Xi), and 6-1 is that lotus root joins estrogenic carrier protein (Xi) or 6-2 is that antiestrogenic antibody (W15) detects trace " | ".
Embodiment 31 and 32, animal body and products thereof steroid hormone quick detection test paper bar (I) and (II).Roughly the same implement one, do not repeat.What some was different is: 3-1 is that steroid hormone gold labeling antibody (W16) or 3-2 are the gold mark carrier protein (Xi) of lotus root connection steroid hormone, and 6-1 is that the carrier protein (Xi) of lotus root connection steroid hormone or the antibody (W16) that 6-2 is the anti-steroid hormone detect trace " | ".
Embodiment 33 and 34, animal body and products thereof sulphadiazine quick detection test paper bar (I) and (II).Roughly the same embodiment one and two does not repeat.What some was different is: 3-1 is that sulphadiazine gold labeling antibody (W17) or 3-2 are the gold mark carrier protein (Xi) of lotus root connection sulphadiazine, and 6-1 is that the carrier protein (Xi) of lotus root connection sulphadiazine or the antibody (W17) that 6-2 is anti-sulphadiazine detect trace " | ".
Embodiment 35 and 36, animal body and products thereof bacteresulf quick detection test paper bar (I) and (II).Roughly the same embodiment one and two does not repeat.What some was different is: 3-1 is that bacteresulf gold labeling antibody (W18) or 3-2 are the gold mark carrier protein (Xi) of lotus root connection bacteresulf, and 6-1 is that the carrier protein (Xi) of lotus root connection bacteresulf or the antibody (W18) that 6-2 is anti-bacteresulf detect trace " | ".
Embodiment 37 and 38, animal body and products thereof nitroimidazole quick detection test paper bar (I) and (II).Roughly the same embodiment one and two does not repeat.What some was different is: 3-1 is that nitroimidazole gold labeling antibody (W19) or 3-2 are the gold mark carrier protein (Xi) of lotus root connection nitroimidazole, and 6-1 is that the carrier protein (Xi) of lotus root connection nitroimidazole or the antibody (W19) that 6-2 is anti-nitroimidazole detect trace " | ".
Embodiment 39 and 40, animal body and products thereof nitrofuran quick detection test paper bar (I) and (II).Roughly the same embodiment one and two does not repeat.What some was different is: 3-1 is that nitrofuran gold labeling antibody (W20) or 3-2 are the gold mark carrier protein (Xi) of lotus root connection nitrofuran, and 6-1 is that the carrier protein (Xi) of lotus root connection nitrofuran or the antibody (W20) that 6-2 is anti-nitrofuran detect trace " | ".
Claims (6)
1. the residual quick test strip I of animal body and products thereof Chinese traditional medicine, contain supporting layer, reaction reagent carrier absorption layer, supporting layer is the strip of foil that do not absorb water, reaction reagent carrier absorption layer is pasted on the supporting layer, it is characterized in that: adsorbed layer is followed successively by fibrage, the absorption corresponding golden labeling antibody of medicine to be checked (Wi) fibrage by the sample end, the cellulose rete, handle end is an absorbent material layer, on the cellulose rete, have contain lotus root join medicine to be detected carrier protein Xi detection trace " | " and the contrast trace " | " that contains sheep or the anti-mouse IgG antibody Y of rabbit is arranged.
2. test strip according to claim 1; it is characterized in that: the supporting layer slip that do not absorb water can be used the hard plastic slip; or the cardboard slip that do not absorb water; fibrage useable glass cotton; absorbent material layer can be used thieving paper; the cellulose rete can be used nitrocellulose filter; gold labeling antibody fibrage can be with golden labeling antibody (Wi) glass wool; gold labeling antibody (Wi) is the monoclonal antibody (Mi) or the polyclonal antibody of colloid gold label; the carrier protein (Xi) that lotus root joins corresponding medicine to be checked is that medicine to be checked and carrier protein lotus root connect the compound that closes; carrier protein can be bovine serum albumin(BSA) (BSA) X1; or be the pure albumen of ovum gallinaceum (OVA) X2; or be ferritin X3; or be hemocyanin X4 etc.; at glass wool; be coated with the plastic cement diaphragm on gold labeling antibody glass wool and the absorbent paper layer, the diaphragm deflection glass wool one side about 0.5cm place corresponding with golden labeling antibody (Wi) intersection at glass wool prints the sample mark line, and the contrast trace on cellulose membrane is " ‖ " with the detection trace and arranges.
3. test strip according to claim 1 and 2, it is characterized in that: be adsorbed with the corresponding golden labeling antibody of certain medicine to be measured (Wi) glass wool, contain detection trace " | " that lotus root joins corresponding pharmaceutical carrier albumen to be checked (Xi) and can be a kind of in the following medicine: penicillin gold labeling antibody (W1) glass wool, lotus root connection penicillin carrier protein (Xi) trace, streptomysin gold labeling antibody (W2) glass wool, lotus root connection streptomysin carrier protein solution (Xi) trace, terramycin gold labeling antibody (W3) glass wool, lotus root connection terramycin carrier protein (Xi) trace, neomycin gold labeling antibody (W4) glass wool, lotus root connection neomycin carrier protein (Xi) trace, erythromycin gold labeling antibody (W5) glass wool, lotus root connection erythromycin carrier protein (Xi) trace, tetracycline gold labeling antibody (W6) glass wool, lotus root connection tetracycline carrier protein (Xi) trace, aureomycin gold labeling antibody (W7) glass wool, lotus root joins golden mould carrier protein (Xi) trace, chloromycetin gold labeling antibody (W8) glass wool, lotus root connection chloromycetin carrier protein (Xi) trace, gentamicin gold labeling antibody (W9) glass wool, lotus root connection gentamicin carrier protein (Xi) trace, kanamycins gold labeling antibody (W10) glass wool, lotus root connection kanamycins carrier protein (Xi) trace, Norfloxacin gold labeling antibody (W11) glass wool, lotus root connection Norfloxacin carrier protein (Xi) trace, Ofloxacin gold labeling antibody (W12) glass wool, lotus root connection Ofloxacin carrier protein (Xi) trace, Ciprofloxacin gold labeling antibody (W13) glass wool, lotus root connection Ciprofloxacin carrier protein trace, bacitracin gold labeling antibody (W14) glass wool, lotus root connection bacitracin carrier protein (Xi) trace, estrogen gold labeling antibody (W15) glass wool, lotus root connection estrogen carrier protein (Xi) trace, steroid hormone gold labeling antibody (W16) glass wool, lotus root connection steroid hormone carrier protein (Xi) trace, sulphadiazine gold labeling antibody (W17) glass wool, lotus root connection sulphadiazine carrier protein (Xi) trace, bacteresulf gold labeling antibody (W18) glass wool, lotus root connection bacteresulf carrier protein (Xi) trace, nitroimidazole gold labeling antibody (W19) glass wool, lotus root connection nitroimidazole carrier protein (Xi) trace, nitrofuran gold labeling antibody (W20) glass wool, lotus root connection nitrofuran carrier protein (Xi) solution trace, gram ball flour gold labeling antibody (W21) glass wool, lotus root connection gram ball powder carrier albumen (Xi) trace, aflatoxin gold labeling antibody (W22) glass wool, lotus root connection aflatoxin carrier protein (Xi) trace, FT gold labeling antibody (W23) glass wool, lotus root connection FT carrier protein (Xi) trace.
4. the residual quick test strip II of animal body and products thereof Chinese traditional medicine, contain supporting layer, reaction reagent carrier absorption layer, supporting layer is fixed on the supporting layer for the strip of foil that do not absorb water, reaction reagent carrier absorption layer, it is characterized in that: adsorbed layer is followed successively by fibrage by the sample end, the absorption lotus root joins gold mark carrier protein (Xi) fibrage of medicine to be checked, the cellulose rete, the handle end absorbent material layer contains the contrast trace " | " that drug antibody to be detected (Wi) detects trace " | " and contains anti-carrier protein Xi antibody on the cellulose rete.
5. test strips II according to claim 4; it is characterized in that: the supporting layer slip that do not absorb water can be the hard plastic slip; or the cardboard slip that does not absorb water; fibrage can be glass wool; absorbent material layer can be used thieving paper; the cellulose rete can be used nitrocellulose filter; gold mark fibrage can join gold mark carrier protein (Xi) glass wool of medicine to be checked with lotus root; lotus root joins the gold of medicine to be checked and marks carrier protein (Xi) joins medicine to be checked for the lotus root of colloid gold label carrier protein; carrier protein (Xi) can be bovine serum albumin(BSA) (BSA) X1; or be (OVA) X2 of the pure albumen of ovum gallinaceum; or be ferritin X3; or be hemocyanin X4 etc.; at glass wool, carry out on gold mark carrier protein (Xi) glass wool and the water accepting layer and be stamped diaphragm, print the sample mark line at the about 0.5cm of the diaphragm deflection glass wool one side place of glass wool and gold mark carrier glass wool intersection correspondence; the combination of detection trace on the cellulose rete and contrast trace is arranged and be can be " ‖ ".
6. according to claim 4 or 5 described test strips II, it is characterized in that: be adsorbed with the gold mark carrier protein Xi glass wool that lotus root joins medicine to be checked, medicine corresponding antibody to be detected (Wi) detects trace " | ", can be a kind of in the following medicine: lotus root connection penicillin gold mark carrier protein Xi glass wool, penicillin antibody W1 trace, lotus root connection streptomysin gold mark carrier protein Xi glass wool, streptomysin antibody W2 trace, lotus root connection terramycin gold mark carrier protein Xi glass wool, terramycin antibody W3 trace, lotus root connection neomycin gold mark carrier protein Xi glass wool, neomycin antibody W4 trace, lotus root connection erythromycin gold mark carrier protein Xi glass wool, erythromycin antibody W5 trace, lotus root connection tetracycline gold mark carrier protein Xi glass wool, tetracycline antibody W6 trace, lotus root connection aureomycin gold mark carrier protein Xi glass wool, chlortetracycline antibody W7 trace, lotus root connection chloromycetin gold mark carrier protein Xi glass wool, chloramphenicol antibody W8 trace, lotus root connection gentamicin gold mark carrier protein Xi glass wool, gentamicin antibody W9 trace, lotus root connection kanamycins gold mark carrier protein Xi glass wool, kanamycins antibody W10 trace, lotus root connection Norfloxacin gold mark carrier protein Xi glass wool, Norfloxacin antibody W11 trace, lotus root connection Ofloxacin gold mark carrier protein Xi glass wool, Ofloxacin antibody W12 trace, lotus root connection Ciprofloxacin gold mark carrier protein Xi glass wool, Ciprofloxacin antibody W13 trace, lotus root connection bacitracin gold mark carrier protein Xi glass wool, bacitracin antibody W14 trace, lotus root connection estrogen gold mark carrier protein Xi glass wool, estrogen antibody W15 trace, lotus root connection steroid hormone gold mark carrier protein Xi glass wool, steroid hormone antibody W16 trace, lotus root connection sulphadiazine gold mark carrier protein Xi glass wool, sulphadiazine antibody W17 trace, lotus root connection bacteresulf gold mark carrier protein Xi glass wool, bacteresulf antibody W18 trace, lotus root connection nitroimidazole gold mark carrier protein Xi glass wool, nitroimidazole antibody W19 trace, lotus root connection nitrofuran gold mark carrier protein Xi glass wool, nitrofuran antibody W20 trace, lotus root connection gram ball flour gold mark carrier protein Xi glass wool, gram ball powder antibody W21 trace, lotus root connection aflatoxin gold mark carrier protein Xi glass wool, aflatoxin antibody W22 trace, lotus root connection FT gold mark carrier protein Xi glass wool, FT antibody W23 trace.
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| CN 02228103 CN2515678Y (en) | 2002-01-10 | 2002-01-10 | Test paper strip for fast detecting drug residue of animal body and its product |
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| CN 02228103 CN2515678Y (en) | 2002-01-10 | 2002-01-10 | Test paper strip for fast detecting drug residue of animal body and its product |
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