CN224040747U - Purification device is used in production of recombinant collagen - Google Patents

Abstract

This utility model discloses a purification device for recombinant collagen production, relating to the field of recombinant collagen production technology. It includes a housing, with a collection hopper fixedly connected to the lower end of the housing. A connecting pipe is fixedly connected to the outer side of the collection hopper, and a filter box is fixedly connected to the other end of the connecting pipe. A support frame is fixedly connected to the upper end of the filter box, and the upper end of the support frame is fixedly connected to the outer side of the housing. A stirring assembly is installed inside the housing. This utility model uses the output of a drive motor to rotate an upper stirring shaft. The rotation of the upper stirring shaft drives a first bevel gear at the lower end to rotate. Through the transmission of a gear, a second bevel gear can easily drive a second stirring shaft to rotate in the opposite direction. This allows multiple stirring rods to rotate in both directions to stir the recombinant collagen cell solution in the housing, ensuring thorough mixing of the raw materials.

Description

Purification device is used in production of recombinant collagen

Technical Field

The utility model relates to the field of recombinant collagen production, in particular to a purification device for recombinant collagen production.

Background

The recombinant collagen is used as a bioactive material with wide application prospect, and plays a key role in various fields such as medicines, cosmetics, foods and the like.

However, in the existing purification device for recombinant collagen production, since the raw material of the recombinant collagen is usually a cell culture solution containing recombinant genes, the components are extremely complex, and various impurities including cell debris, unreacted culture medium components, foreign proteins, nucleic acids, endotoxin and the like are contained, the conventional device often lacks efficient stirring and cell disruption synergistic mechanisms, so that the recombinant collagen in the cells cannot be fully released, and the impurities are difficult to uniformly disperse, the subsequent purification effect is greatly affected, and in the filtration stage, the existing filter assembly is difficult to conveniently replace and maintain, and when the filter assembly is blocked or the filtration effect is reduced, a great amount of time and labor are consumed for disassembly, cleaning or replacement, so that the production efficiency is seriously affected.

Disclosure of utility model

The utility model mainly aims to provide a purification device for producing recombinant collagen, which can effectively solve the problems in the background technology.

In order to achieve the above purpose, the technical scheme adopted by the utility model is as follows:

The utility model provides a purification device is used in production of recombinant collagen, includes the box, the lower extreme fixedly connected with collecting hopper of box, collecting hopper's outside fixedly connected with connecting pipe, the other end fixedly connected with rose box of connecting pipe, the upper end fixedly connected with support frame of rose box, the upper end fixedly connected with of support frame is in the outside of box, the inner chamber of box is provided with stirring subassembly, stirring subassembly's outside is provided with the ultrasonic breaker, ultrasonic breaker fixed mounting is on the inner wall of box, the inboard of rose box is provided with filtering subassembly, one side fixedly connected with discharging pipe of rose box, the discharging pipe is located filtering subassembly's below, the feed inlet has been seted up to the upper end of box, one side of rose box is provided with spacing subassembly, filtering subassembly passes through spacing subassembly fixed joint in the inboard of rose box.

Preferably, the stirring assembly comprises a driving motor, the driving motor is arranged at the upper end of the box body, an upper stirring shaft is fixedly connected to the output end of the driving motor, the upper stirring shaft is rotationally connected with the box body, a first bevel gear is fixedly connected to the lower end of the upper stirring shaft, a plurality of stirring rods are fixedly connected to the outer side of the upper stirring shaft, and the stirring rods are all arranged in the inner cavity of the box body.

Preferably, the inner chamber of box is last fixedly connected with mount pad, one side of mount pad rotates and is connected with drive gear, drive gear and first bevel gear meshing are connected, drive gear's the outside still meshes there is the second bevel gear, the lower extreme fixedly connected with second (mixing) shaft of second bevel gear, the second (mixing) shaft rotates with the inner wall lower extreme of box to be connected, the outside of second (mixing) shaft also fixedly connected with a plurality of puddlers, a plurality of the inner chamber of box is all located to the puddler.

Preferably, the filter component comprises a filter frame, the filter frame is slidably connected to the inner side of the filter box, a handle is fixedly connected to one side of the filter frame, the handle is arranged on the outer side of the filter box, a microfiltration membrane filter screen is fixedly arranged on the inner side of the filter frame, and the lower end of the microfiltration membrane filter screen is provided with an ultrafiltration membrane filter screen fixedly arranged on the filter frame.

Preferably, the spacing subassembly includes the sleeve, sleeve fixed mounting is in one side of rose box, telescopic inboard sliding connection has the gag lever post, the outside fixedly connected with plectane of gag lever post, plectane sliding connection is in telescopic inboard, one side fixedly connected with spring of plectane, the other end and the sleeve fixed connection of spring, the one end fixedly connected with pull ring of gag lever post, the telescopic outside is located to the pull ring, spacing hole has been seted up in the outside of filter frame, the inner chamber in spacing hole is located to the other end of gag lever post.

Preferably, a valve is arranged on the outer side of the connecting pipe.

Preferably, the outside fixedly connected with installation piece of driving motor, installation piece fixed mounting is in the upper end of box.

Compared with the prior art, the utility model has the following beneficial effects:

1. This purification device is used in production of recombinant collagen, output through driving motor will drive the (mixing) shaft and rotate, go up the (mixing) shaft and rotate and will drive the first bevel gear of lower extreme and rotate, at this moment through drive gear's transmission cooperation, realization second bevel gear that can be convenient drives the second (mixing) shaft and carry out reverse rotation, and then can realize that a plurality of puddlers forward and reverse rotations stir the cell solution of recombinant collagen in the box, ensure raw materials intensive mixing, in the stirring, start the ultrasonic breaker of fixed mounting on the box inner wall, the ultrasonic breaker utilizes the cavitation effect of ultrasonic wave, carry out the breakage to the cell in the raw materials, make the abundant release of intracellular recombinant collagen in solution, stirring and ultrasonic broken synergism, recombinant collagen's release efficiency and homogeneity have been improved.

2. This purification device is used in production of recombinant collagen, when needs change or maintain filter unit, through pulling the pull ring, make the gag lever post slide in the sleeve, the plectane in gag lever post outside slides and compresses the spring thereupon, until the other end of gag lever post deviate from in the spacing hole of seting up in the filter frame outside, at this moment, the spacing between filter frame and the rose box is relieved, can be convenient take out filter frame from the inboard of rose box, carry out maintenance operations such as washing, change filter screen.

Drawings

FIG. 1 is a schematic diagram showing the overall structure of a purification apparatus for producing recombinant collagen according to the present utility model;

FIG. 2 is a schematic diagram showing the overall structure of a purification apparatus for producing recombinant collagen according to the present utility model;

FIG. 3 is a cross-sectional view showing the overall structure of a purification apparatus for recombinant collagen production according to the present utility model;

FIG. 4 is a schematic diagram of a partially exploded structure of a purification device for recombinant collagen production according to the present utility model;

FIG. 5 is an enlarged schematic view of the structure of the purification device for producing recombinant collagen according to the present utility model.

The ultrasonic crusher comprises a box body 1, a supporting frame 2, a collecting hopper 3, a connecting pipe 4, a valve 5, a filter box 6, a filter assembly 7, a filter assembly 71, a filter frame 72, a microfiltration membrane filter screen 73, an ultrafiltration membrane filter screen 8, a handle 9, a limiting assembly 91, a sleeve 92, a limiting rod 93, a circular plate 94, a spring 95, a pull ring 96, a limiting hole 10, a driving motor 11, a mounting block 12, an upper stirring shaft 13, a first bevel gear 14, a transmission gear 15, a second bevel gear 16, a second stirring shaft 17, a stirring rod 18, a discharging pipe 19 and an ultrasonic crusher.

Detailed Description

The utility model is further described in connection with the following detailed description, in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the utility model easy to understand.

As shown in fig. 1-5, a purification device for recombinant collagen production comprises a box body 1, the lower extreme fixedly connected with collecting hopper 3 of box body 1, the outside fixedly connected with connecting pipe 4 of collecting hopper 3, the other end fixedly connected with rose box 6 of connecting pipe 4, the upper end fixedly connected with support frame 2 of rose box 6, the upper end fixedly connected with of support frame 2 is in the outside of box body 1, the inner chamber of box body 1 is provided with stirring subassembly, the outside of stirring subassembly is provided with ultrasonic breaker 19, ultrasonic breaker 19 fixed mounting is on the inner wall of box body 1, the inboard of rose box 6 is provided with filter subassembly 7, one side fixedly connected with discharging pipe 18 of rose box 6, discharging pipe 18 is located the below of filter subassembly 7, the feed inlet has been seted up to the upper end of box body 1, one side of rose box 6 is provided with spacing subassembly 9, filter subassembly 7 passes through spacing subassembly 9 fixed joint in the inboard of rose box 6.

In this embodiment, the stirring subassembly includes driving motor 10, driving motor 10 locates the upper end of box 1, driving motor 10's output so fixedly connected with goes up (mixing) shaft 12, go up (mixing) shaft 12 and box 1 rotation connection, go up (mixing) shaft 12's lower extreme fixedly connected with first bevel gear 13, go up (mixing) shaft 12's outside fixedly connected with a plurality of puddlers 17, the inner chamber of box 1 is all located to a plurality of puddlers 17, fixedly connected with mount pad on the inner chamber of box 1, one side rotation of mount pad is connected with drive gear 14, drive gear 14 and first bevel gear 13 meshing are connected, the outside of drive gear 14 still meshes with second bevel gear 15, second (mixing) shaft 16) is connected with in rotation to second bevel gear 15's lower extreme, second (mixing) shaft 16 and box 1's inner wall lower extreme rotation connection, a plurality of puddlers 17 are also fixedly connected with in the outside of second (mixing) shaft 16, a plurality of puddlers 17 all locate the inner chamber of box 1.

Specifically, through the feed inlet that box 1 upper end was seted up, can throw into the inner chamber of box 1 with the cell solution or other relevant mixture raw materials that contain recombinant collagen, start driving motor 10 this moment, driving motor 10's output will drive upper mixing shaft 12 and rotate, upper mixing shaft 12 rotates and will drive the first bevel gear 13 of lower extreme and rotate, at this moment, drive the cooperation through drive gear 14 at the second, can conveniently realize that second bevel gear 15 drives second (16) and rotate in opposite directions, and then can realize that a plurality of puddlers 17 forward and reverse rotates and stir the cell solution of recombinant collagen in box 1, ensure that the raw materials intensive mixing, at the same time of stirring, the ultrasonic breaker 19 utilizes the cavitation effect of ultrasonic wave, carry out the breakage to the cell in the raw materials, make the inside recombinant collagen of cell fully release in the solution, stirring and ultrasonic breakage's synergism, recombinant collagen's release efficiency and homogeneity have been improved.

In this embodiment, the filter assembly 7 includes a filter frame 71, the filter frame 71 is slidably connected to the inner side of the filter box 6, a handle 8 is fixedly connected to one side of the filter frame 71, the handle 8 is disposed on the outer side of the filter box 6, a microfiltration membrane filter screen 72 is fixedly mounted on the inner side of the filter frame 71, and an ultrafiltration membrane filter screen 73 fixedly mounted on the filter frame 71 is disposed at the lower end of the microfiltration membrane filter screen 72.

Specifically, through the design of connecting pipe 4, can be convenient pour into rose box 6 with solution into rose box 6, after the solution gets into rose box 6, contact micro-filtration membrane filter screen 72 at first and carry out preliminary filtration, can effectively get rid of the relatively big granule impurity such as incomplete broken cell debris, big insoluble substance, then filter at milipore filter screen 73 again, the impurity of relatively big molecular mass, such as partial miscellaneous protein, nucleic acid is blocked, let the recombinant collagen solution pass through simultaneously, realize preliminary purification, the solution will flow out rose box 6 through discharging pipe 18 after filtering, so that collect and be used for follow-up further purification treatment.

In this embodiment, spacing subassembly 9 includes sleeve 91, sleeve 91 fixed mounting is in one side of rose box 6, the inboard sliding connection of sleeve 91 has gag lever post 92, the outside fixedly connected with plectane 93 of gag lever post 92, plectane 93 sliding connection is in the inboard of sleeve 91, one side fixedly connected with spring 94 of plectane 93, the other end and the sleeve 91 fixed connection of spring 94, the one end fixedly connected with pull ring 95 of gag lever post 92, the outside of sleeve 91 is located to pull ring 95, spacing hole 96 has been seted up in the outside of filter frame 71, the inner chamber of spacing hole 96 is located to the other end of gag lever post 92.

Specifically, after a period of time of filtration, the filter assembly 7 will need to be replaced or maintained, at this time, the operator pulls the pull ring 95 to make the stop lever 92 slide in the sleeve 91, the circular plate 93 at the outer side of the stop lever 92 slides along with the stop lever and compresses the spring 94 until the other end of the stop lever 92 is separated from the stop hole 96 formed at the outer side of the filter frame 71, at this time, the limit between the filter frame 71 and the filter box 6 is released, the operator can pull the handle 8 to pull the filter frame 71 out of the inner side of the filter box 6 to perform maintenance operations such as cleaning and replacing a filter screen, and the like, conversely, the filter frame 71 is slid into the inner side of the filter box 6 again, the pull ring 95 is released, the circular plate 93 drives the stop lever 92 to reset under the elastic force of the spring 94, and the other end of the stop lever 92 is reinserted into the inner cavity of the stop hole 96 to fix the filter assembly 7 in the inner side of the filter box 6.

In this embodiment, the valve 5 is provided outside the connection pipe 4.

Specifically, through the design of valve 5, can make things convenient for the staff to control the velocity of flow of solution in connecting pipe 4 as required, it is more convenient.

In this embodiment, the outer side of the driving motor 10 is fixedly connected with a mounting block 11, and the mounting block 11 is fixedly mounted at the upper end of the case 1.

Specifically, by the design of the mounting block 11, the stability of the driving motor 10 at the time of operation can be improved.

It should be noted that, the present utility model is a purification device for producing recombinant collagen, a user firstly puts a cell solution containing recombinant collagen or other related mixture raw materials into an inner cavity of a box 1 through a feed inlet formed at the upper end of the box 1, at this time, a driving motor 10 is started, an output end of the driving motor 10 drives an upper stirring shaft 12 to rotate, the upper stirring shaft 12 rotates to drive a first bevel gear 13 at the lower end to rotate, at this time, through transmission cooperation of a transmission gear 14, the second bevel gear 15 can conveniently realize that the second stirring shaft 16 drives the second stirring shaft 16 to reversely rotate, further, a plurality of stirring rods 17 can realize forward and reverse rotation to stir the cell solution of the recombinant collagen in the box 1, so as to ensure that the raw materials are fully mixed, at the same time of stirring, an ultrasonic breaker 19 fixedly installed on the inner wall of the box 1 is started, the ultrasonic breaker 19 breaks cells in the raw materials by utilizing cavitation effect of ultrasonic waves, so that recombinant collagen in the cells is fully released into the solution, the synergistic effect of stirring and ultrasonic breaking improves the release efficiency and uniformity of the recombinant collagen, at the moment, the valve 5 arranged on the outer side of the connecting pipe 4 is opened, so that the solution can flow to the filter box 6 through the connecting pipe 4, after the solution enters the filter box 6, the solution firstly contacts the micro-filtration membrane filter screen 72 for preliminary filtration, larger particle impurities such as incompletely broken cell fragments and large insoluble substances can be effectively removed, then the impurities with relatively large molecular mass such as partial impurity proteins and nucleic acids are filtered again in the ultra-filtration membrane filter screen 73, meanwhile, the recombinant collagen solution is allowed to pass through, the preliminary purification is realized, the filtered solution flows out of the filter box 6 through the discharging pipe 18, after a period of filtering, the filter assembly 7 needs to be replaced or maintained, at this time, an operator pulls the pull ring 95 to enable the limiting rod 92 to slide in the sleeve 91, the circular plate 93 at the outer side of the limiting rod 92 slides along with the limiting rod and compresses the spring 94 until the other end of the limiting rod 92 is separated from the limiting hole 96 formed at the outer side of the filter frame 71, at this time, the limiting between the filter frame 71 and the filter box 6 is released, and the operator can pull the handle 8 to pull the filter frame 71 out of the inner side of the filter box 6 for maintenance operations such as cleaning and replacing the filter screen, and conversely, slide the filter frame 71 back into the inner side of the filter box 6, release the pull ring 95, and under the action of the elastic force of the spring 94, the circular plate 93 drives the limiting rod 92 to reset, and the other end of the limiting rod 92 is reinserted into the inner cavity of the limiting hole 96 to fix the filter assembly 7 on the inner side of the filter box 6.

The foregoing has shown and described the basic principles and main features of the present utility model and the advantages of the present utility model. It will be understood by those skilled in the art that the present utility model is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present utility model, and various changes and modifications may be made without departing from the spirit and scope of the utility model, which is defined in the appended claims. The scope of the utility model is defined by the appended claims and equivalents thereof.

Claims (7)

1. The purifying device for producing the recombinant collagen comprises a box body (1) and is characterized in that the lower end of the box body (1) is fixedly connected with a collecting hopper (3), the outer side of the collecting hopper (3) is fixedly connected with a connecting pipe (4), the other end of the connecting pipe (4) is fixedly connected with a filter box (6), the upper end of the filter box (6) is fixedly connected with a supporting frame (2), the upper end of the supporting frame (2) is fixedly connected to the outer side of the box body (1), an inner cavity of the box body (1) is provided with a stirring assembly, the outer side of the stirring assembly is provided with an ultrasonic crusher (19), the ultrasonic crusher (19) is fixedly arranged on the inner wall of the box body (1), the inner side of the filter box (6) is provided with a filter assembly (7), one side of the filter box (6) is fixedly connected with a discharging pipe (18), the lower end of the filter box (6) is fixedly connected with a supporting frame (2), the upper end of the box body (1) is provided with a feeding port, one side of the filter box (6) is provided with a clamping assembly (9), and the inner side of the filter assembly (9) is fixedly connected to the filter assembly (9) through the clamping assembly.

2. The purification device for producing recombinant collagen according to claim 1, wherein the stirring assembly comprises a driving motor (10), the driving motor (10) is arranged at the upper end of the box body (1), an upper stirring shaft (12) is fixedly connected with the output end of the driving motor (10), the upper stirring shaft (12) is rotationally connected with the box body (1), a first bevel gear (13) is fixedly connected with the lower end of the upper stirring shaft (12), a plurality of stirring rods (17) are fixedly connected with the outer side of the upper stirring shaft (12), and the stirring rods (17) are all arranged in the inner cavity of the box body (1).

3. The purification device for producing recombinant collagen according to claim 2, wherein an installation seat is fixedly connected to the inner cavity of the box body (1), one side of the installation seat is rotatably connected with a transmission gear (14), the transmission gear (14) is in meshed connection with the first bevel gear (13), a second bevel gear (15) is further meshed with the outer side of the transmission gear (14), a second stirring shaft (16) is fixedly connected to the lower end of the second bevel gear (15), the second stirring shaft (16) is rotatably connected with the lower end of the inner wall of the box body (1), a plurality of stirring rods (17) are fixedly connected to the outer side of the second stirring shaft (16), and the stirring rods (17) are all arranged in the inner cavity of the box body (1).

4. The purification device for recombinant collagen production according to claim 1, wherein the filter assembly (7) comprises a filter frame (71), the filter frame (71) is slidably connected to the inner side of the filter box (6), a handle (8) is fixedly connected to one side of the filter frame (71), the handle (8) is arranged on the outer side of the filter box (6), a microfiltration membrane filter screen (72) is fixedly arranged on the inner side of the filter frame (71), and an ultrafiltration membrane filter screen (73) fixedly arranged on the filter frame (71) is arranged at the lower end of the microfiltration membrane filter screen (72).

5. The purification device for producing recombinant collagen according to claim 4, wherein the limiting component (9) comprises a sleeve (91), the sleeve (91) is fixedly arranged on one side of the filter box (6), a limiting rod (92) is slidably connected to the inner side of the sleeve (91), a circular plate (93) is fixedly connected to the outer side of the limiting rod (92), the circular plate (93) is slidably connected to the inner side of the sleeve (91), a spring (94) is fixedly connected to one side of the circular plate (93), the other end of the spring (94) is fixedly connected with the sleeve (91), a pull ring (95) is fixedly connected to one end of the limiting rod (92), the pull ring (95) is arranged on the outer side of the sleeve (91), a limiting hole (96) is formed in the outer side of the filter frame (71), and the other end of the limiting rod (92) is arranged in an inner cavity of the limiting hole (96).

6. The purification device for recombinant collagen production according to claim 1, wherein a valve (5) is provided outside the connecting tube (4).

7. The purification device for producing recombinant collagen according to claim 2, wherein the outer side of the driving motor (10) is fixedly connected with a mounting block (11), and the mounting block (11) is fixedly arranged at the upper end of the box body (1).