CN223832352U - Needle positioning mechanism and micro-droplet generation device - Google Patents
Needle positioning mechanism and micro-droplet generation deviceInfo
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- CN223832352U CN223832352U CN202520025958.XU CN202520025958U CN223832352U CN 223832352 U CN223832352 U CN 223832352U CN 202520025958 U CN202520025958 U CN 202520025958U CN 223832352 U CN223832352 U CN 223832352U
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Abstract
The utility model discloses a needle positioning mechanism and a micro-droplet generation device, and relates to the technical field of nucleic acid detection. The needle positioning mechanism comprises a needle tube, a sleeve, a needle and an outer catheter. The sleeve is connected with the needle tube, and the sleeve is located outside the needle tube, form the oil feed passageway between sleeve and the needle tube, the syringe needle is installed in the tip of needle tube, outer pipe is installed in telescopic tip, and the sleeve is located outside the syringe needle, form out the oil channel between outer pipe and the syringe needle, out the oil channel and advance oil channel intercommunication, the outer wall of syringe needle is provided with first location rib, first location rib extends along the length direction of syringe needle and sets up, first location rib is used for spacing to outer pipe when outer pipe installation. The needle positioning mechanism provided by the utility model can improve the centering precision of the outer catheter and the needle, ensure the same proportion of the original solution in each water-in-oil micro-droplet, improve the uniformity of the water-in-oil micro-droplet and improve the nucleic acid detection precision.
Description
Technical Field
The utility model relates to the technical field of nucleic acid detection, in particular to a needle positioning mechanism and a micro-droplet generation device.
Background
The microdroplet digital PCR is an absolute quantitative PCR technology, which mainly adopts a microdroplet method to disperse a solution containing target nucleic acid into a large number of water-in-oil micro-droplets to form tens of thousands of independent micro-reaction systems with equal volumes, wherein the number of initial target nucleic acid templates of each droplet is 0, 1 or more, so that after PCR circulation, the droplet with one target nucleic acid initially gives out a fluorescent signal, the droplet without target nucleic acid has no fluorescent signal, and the concentration of the target nucleic acid of the original solution can be calculated by mathematical correction according to the relative proportion of the number of the two droplets and poisson distribution.
The existing preparation method of the water-in-oil micro-droplets generally comprises the steps of mixing an original solution and oil liquid according to a certain proportion, then carrying out spiral stirring, and then dripping out according to a certain flow velocity to form the water-in-oil micro-droplets. However, the preparation method of spiral stirring easily causes uneven mixing of the original solution and the oil liquid, so that the original solution in each water-in-oil micro-droplet has different duty ratios, the uniformity of the water-in-oil micro-droplet is poor, and the nucleic acid detection precision is directly affected.
In view of the above, it is important to design and manufacture a needle positioning mechanism with high degree of alignment and good uniformity, and a micro-droplet generation device, especially in nucleic acid detection.
Disclosure of utility model
The utility model aims to provide a needle positioning mechanism which can improve the centering precision of an outer catheter and a needle, ensure the same proportion of original solution in each water-in-oil micro-droplet, improve the uniformity of the water-in-oil micro-droplet and improve the detection precision of nucleic acid.
The utility model further aims to provide a micro-droplet generation device, which can improve the centering precision of an outer catheter and a needle head, ensure that the original solution in each water-in-oil micro-droplet has the same ratio, improve the uniformity of the water-in-oil micro-droplet and improve the detection precision of nucleic acid.
The utility model is realized by adopting the following technical scheme.
The utility model provides a syringe needle positioning mechanism, including needle tubing, sleeve, syringe needle and outer pipe, the sleeve is connected with the needle tubing, and the cover is located outside the needle tubing, form into oil feed passageway between sleeve and the needle tubing, the tip in the needle tubing is installed to the syringe needle, outer pipe is installed in telescopic tip, and the cover is located outside the syringe needle, form out oil channel between outer pipe and the syringe needle, out oil channel and oil feed passageway intercommunication, the outer wall of syringe needle is provided with first location rib, first location rib extends along the length direction of syringe needle and sets up, first location rib is used for spacing to outer pipe when outer pipe is installed.
Optionally, the outer catheter includes assembly section, linkage segment and the throat section that connects gradually, and assembly section cover locates outside the sleeve, and with sleeve joint, the syringe needle sets up in linkage segment and throat section simultaneously, and first positioning rib cooperates with the linkage segment.
Optionally, the syringe needle includes cooperation section, connection section and the convergent section that connects gradually, and cooperation section cover is located outside the needle tubing, and with needle tubing joint, first location rib sets up simultaneously on cooperation section and connection section, and convergent section part stretches into the setting of throat section.
Optionally, be provided with the second location rib in the throat section, the second location rib extends along the length direction of outer pipe and sets up, and the second location rib is used for spacing the convergent section when outer pipe installs.
Optionally, the necking section is internally provided with a necking inner wall and a straight cylinder inner wall in sequence, one end of the tapering section, which is far away from the connecting section, is extended and provided with a capillary tube, the second positioning rib is arranged on the necking inner wall, and the capillary tube part extends into the straight cylinder inner wall and is coaxially arranged at intervals with the straight cylinder inner wall.
Optionally, the number of the second positioning ribs is multiple, and the multiple second positioning ribs are distributed in an annular array.
Optionally, a limiting table is arranged in the connecting section, a step part is arranged at one end of the matching section, which is far away from the connecting section, and the step part is propped against the limiting table.
Optionally, the step portion is provided with an oil leakage hole, the oil leakage hole and the first positioning rib are arranged in a staggered mode, and the oil leakage hole is used for oil supply.
Optionally, the number of the first positioning ribs is multiple, and the multiple first positioning ribs are distributed in an annular array.
The utility model provides a little liquid droplet generation device, including foretell syringe needle positioning mechanism, this syringe needle positioning mechanism includes the needle tubing, the sleeve, syringe needle and outer pipe, sleeve and needle tube connection, and the cover is located outside the needle tubing, form into oil feed passageway between sleeve and the needle tubing, the tip in the needle tubing is installed to the syringe needle, outer pipe is installed in telescopic tip, and the cover is located outside the syringe needle, form out the oil channel between outer pipe and the syringe needle, oil channel and oil feed passageway intercommunication, the outer wall of syringe needle is provided with first location rib, first location rib extends along the length direction of syringe needle and sets up, first location rib is used for spacing to outer pipe when outer pipe installation.
The needle positioning mechanism and the micro-droplet generating device provided by the utility model have the following
The beneficial effects are that:
According to the needle positioning mechanism provided by the utility model, the sleeve is connected with the needle tube and sleeved outside the needle tube, an oil inlet channel is formed between the sleeve and the needle tube, the needle is arranged at the end part of the needle tube, the outer guide tube is arranged at the end part of the sleeve and sleeved outside the needle tube, an oil outlet channel is formed between the outer guide tube and the needle tube, the oil outlet channel is communicated with the oil inlet channel, the outer wall of the needle tube is provided with the first positioning ribs, the first positioning ribs are arranged in an extending manner along the length direction of the needle tube, and the first positioning ribs are used for limiting the outer guide tube during the installation of the outer guide tube. Compared with the prior art, the needle positioning mechanism provided by the utility model has the advantages that as the first positioning ribs arranged on the outer wall of the needle and the outer guide pipe matched with the first positioning ribs are adopted, the centering precision of the outer guide pipe and the needle can be improved, the same ratio of the original solution in each water-in-oil micro-droplet is ensured, the uniformity of the water-in-oil micro-droplet is improved, and the nucleic acid detection precision is improved.
The micro-droplet generation device provided by the utility model comprises the needle positioning mechanism, so that the centering precision of the outer catheter and the needle can be improved, the same proportion of the original solution in each water-in-oil micro-droplet is ensured, the uniformity of the water-in-oil micro-droplet is improved, and the nucleic acid detection precision is improved.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present utility model and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
Fig. 1 is a schematic structural diagram of a micro-droplet generating device according to an embodiment of the present utility model;
FIG. 2 is a cross-sectional view of a needle positioning mechanism provided in an embodiment of the present utility model;
FIG. 3 is a cross-sectional view of a needle positioning mechanism provided in an embodiment of the present utility model;
FIG. 4 is a cross-sectional view of the outer catheter mated with the needle in the needle positioning mechanism provided by the embodiment of the present utility model;
FIG. 5 is a cross-sectional view of the outer catheter mated with the needle in the needle positioning mechanism provided by the embodiment of the present utility model;
FIG. 6 is a cross-sectional view of an outer catheter of a needle positioning mechanism according to an embodiment of the present utility model;
Fig. 7 is a cross-sectional view of a needle in a needle positioning mechanism according to an embodiment of the present utility model mated with an outer catheter disposed on a carrier plate.
The icons comprise a 10-micro-droplet generating device, a 100-needle positioning mechanism, a 110-needle tube, a 120-sleeve, a 130-needle, a 131-first positioning rib, a 132-first gap, a 133-matching section, a 134-connecting section, a 135-tapering section, a 136-capillary, a 137-step part, a 138-oil leakage hole, a 140-outer conduit, a 141-assembling section, a 142-connecting section, a 143-necking section, a 1431-necking inner wall, a 1432-straight cylinder inner wall, a 144-second positioning rib, a 146-second gap, a 147-limiting table, a 148-hanging lug, a 150-oil inlet channel, a 160-oil outlet channel, a 200-driving mechanism, a 300-push rod, a 400-bearing disc and a 410-through hole.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present utility model more apparent, the technical solutions of the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model, and it is apparent that the described embodiments are some embodiments of the present utility model, but not all embodiments of the present utility model. The components of the embodiments of the present utility model generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the utility model, as presented in the figures, is not intended to limit the scope of the utility model, as claimed, but is merely representative of selected embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
It should be noted that like reference numerals and letters refer to like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.
In the description of the present utility model, it should be noted that, directions or positional relationships indicated by terms such as "inner", "outer", "upper", "lower", "horizontal", etc., are directions or positional relationships based on those shown in the drawings, or those that are conventionally put in use of the inventive product, are merely for convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the apparatus or elements referred to must have a specific direction, be configured and operated in a specific direction, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," "third," and the like are used merely to distinguish between descriptions and should not be construed as indicating or implying relative importance.
In the description of the present utility model, it should also be noted that, unless explicitly specified and limited otherwise, the terms "disposed," "connected," "mounted," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, integrally connected, mechanically connected, electrically connected, directly connected, indirectly connected through an intermediary, or in communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.
Some embodiments of the present utility model are described in detail below with reference to the accompanying drawings. Features of the embodiments described below may be combined with each other without conflict.
Referring to fig. 1 to 3, a droplet generation device 10 for generating water-in-oil droplets for nucleic acid detection is provided in an embodiment of the utility model. The centering accuracy of the outer guide pipe 140 and the needle 130 can be improved, the same proportion of the original solution in each water-in-oil micro-droplet is ensured, the uniformity of the water-in-oil micro-droplet is improved, and the nucleic acid detection accuracy is improved.
The micro-droplet generator 10 is applied to a digital PCR detection apparatus (not shown), and the digital PCR detection apparatus further includes a temperature control device (not shown) and a microscopic photographing device (not shown). The micro-droplet generation device 10 is used for preparing an original solution in a sample into a large number of water-in-oil micro-droplets, injecting the water-in-oil micro-droplets into a reaction disk arranged on a temperature control device, wherein the temperature control device is used for carrying out temperature rise and fall circulation on the water-in-oil micro-droplets through the reaction disk, the number of target nucleic acids in each water-in-oil micro-droplet is doubled every time the temperature rise and fall circulation is carried out, the steps are repeated in such a way that the number of trace target nucleic acids is greatly increased, the micro-photographing device is used for photographing and measuring all the water-in-oil micro-droplets after the temperature rise and fall circulation is completed, the water-in-oil micro-droplets with the target nucleic acids initially give fluorescent signals, the water-in-oil micro-droplets without the target nucleic acids do not have fluorescent signals, and the target nucleic acid concentration of the original solution in the corresponding sample can be calculated according to the relative proportion of the number of the two water-in-oil micro-droplets and the mathematical correction of the loose distribution.
The micro-droplet generation device 10 includes a drive mechanism 200, a pushrod 300, an oil pump mechanism (not shown), and a needle positioning mechanism 100. Wherein, push rod 300 slidably sets up in syringe needle positioning mechanism 100, and push rod 300 can slide for syringe needle positioning mechanism 100 to form negative pressure or malleation in making syringe needle positioning mechanism 100, thereby realize imbibition function and dropping liquid function, syringe needle positioning mechanism 100 can lead and spacing to push rod 300. The driving mechanism 200 is connected with the push rod 300, and the driving mechanism 200 is used for driving the push rod 300 to slide relative to the needle positioning mechanism 100 so as to realize automatic control of the push rod 300. The oil pump mechanism is connected with the needle positioning mechanism 100, and is used for introducing flushing oil into the needle positioning mechanism 100 so as to form water-in-oil micro-droplets.
Needle positioning mechanism 100 includes needle cannula 110, hub 120, needle 130, and outer catheter 140. The sleeve 120 is connected with the needle tube 110 and sleeved outside the needle tube 110, an oil inlet channel 150 is formed between the sleeve 120 and the needle tube 110, and the oil inlet channel 150 is coaxial with and separated from the inner cavity of the needle tube 110, so that the oil inlet channel 150 and the inner cavity of the needle tube 110 are mutually independent and are not affected, wherein the oil inlet channel 150 is used for supplying flushing oil. The push rod 300 is slidably disposed in the needle tube 110, the push rod 300 can slide relative to the needle tube 110, so that negative pressure or positive pressure is formed in the needle tube 110, a liquid sucking function and a liquid dropping function are realized, and the needle tube 110 can guide and limit the push rod 300. The needle 130 is mounted at the end of the needle tube 110, the needle 130 is communicated with the inner cavity of the needle tube 110, the outer conduit 140 is mounted at the end of the sleeve 120 and sleeved outside the needle 130, an oil outlet channel 160 is formed between the outer conduit 140 and the needle 130, the oil outlet channel 160 is communicated with the oil inlet channel 150, and flushing oil in the oil inlet channel 150 can smoothly flow to the oil outlet channel 160. Specifically, the needle 130 is used for extruding the original solution when the push rod 300 slides towards the direction close to the needle 130, the oil outlet channel 160 is used for outputting flushing oil outwards while extruding the original solution to form water-in-oil micro-droplets, and through the limiting action of the needle 130 and the outer conduit 140 and reasonably controlling the flow rates of the original solution and the flushing oil, the original solution and the flushing oil can generate coaxial sheath flow (the original solution flows out of the needle 130 and the flushing oil flows out of the oil outlet channel 160) and form the water-in-oil micro-droplets, so that the same proportion of the original solution in each water-in-oil micro-droplet is ensured, the uniformity of the water-in-oil micro-droplets is improved, and the nucleic acid detection precision is improved.
In the use process of the micro-droplet generation device 10, firstly, air in the needle tube 110 and the needle 130 is removed, then a certain amount of original solution is sucked through the needle 130, then the original solution flows outwards from the needle 130 under the pressure of the push rod 300 to form micro-droplets, in the process, flushing oil uniformly enters the oil inlet channel 150 under the action of external force and outwards flows out through the oil outlet channel 160 to flush the micro-droplets positioned at the end part of the needle 130, and when the acting force (flushing force) of the flushing oil is larger than the surface tension of the micro-droplets, the micro-droplets fall off from the end part of the needle 130 and flow into a reaction disc below along with the flushing oil, so that an original solution droplet (namely, a water-in-oil micro-droplet) wrapped by the flushing oil is formed. Specifically, as long as the flow rates of the original solution and the flushing oil are uniform (the flow rates of the two liquids are different), uniform water-in-oil microdroplets can be formed, the size of the water-in-oil microdroplets is influenced by the flow rates and physical properties (viscosity, density and the like) of the two liquids, and if water-in-oil microdroplets with different sizes are to be generated, the flow rates and the component proportions of the two liquids need to be adjusted.
The needle 130 and the outer catheter 140 are both consumable. In the dripping process, the needle 130 is required to be mounted on the needle tube 110, the needle 130 is extended into the sample solution storage tube to suck the original solution in the sample solution storage tube into the needle 130 (the sucked amount is small, about 20 mu L, so that the original solution cannot enter the needle tube 110), the outer guide tube 140 is mounted on the sleeve 120, the outer guide tube 140 is sleeved outside the needle 130 at intervals, an oil outlet channel 160 is formed between the outer guide tube 140 and the needle 130, the original solution is dripped outwards, and flushing oil is synchronously introduced into the oil inlet channel 150, so that the flushing oil flows out of the oil outlet channel 160 and is wrapped outside the original solution droplets to form water-in-oil micro-droplets. Thus, during each drip, only the needle 130 and outer tube 140 are in contact with the original solution of the sample, and to avoid cross-contamination of multiple samples, it is necessary to remove the used needle 130 and outer tube 140 after each drip is completed and to replace the needle 130 and outer tube 140 with a new one for the next drip.
Referring to fig. 4 to 7 in combination, it is noted that the outer wall of the needle 130 is provided with a first positioning rib 131, the first positioning rib 131 extends along the length direction of the needle 130, and the first positioning rib 131 is used for limiting the outer catheter 140 when the outer catheter 140 is installed, so as to fine-tune the position of the outer catheter 140, improve the centering accuracy of the outer catheter 140 and the needle 130, ensure that the outer catheter 140 is centered with the needle 130 after the installation is completed, thereby improving the coaxiality of the oil outlet channel 160 and the needle 130, further improving the coaxial sheath flow effect of the original solution and the flushing oil, and ensuring that the water-in-oil micro droplets drop out uniformly.
Specifically, the number of the first positioning ribs 131 is multiple, the first positioning ribs 131 are distributed in a ring array, a first gap 132 is formed between two adjacent first positioning ribs 131, the first gap 132 is used for allowing flushing oil to circulate, so that the first positioning ribs 131 are prevented from affecting the flow of the flushing oil in the oil outlet channel 160, and the first gaps 132 act together to ensure that the flow rate of the flushing oil can meet the requirement of dropping liquid.
The outer catheter 140 includes a fitting section 141, a connecting section 142, and a reducing section 143 connected in sequence. In this embodiment, the assembly section 141, the connection section 142 and the necking section 143 are integrally formed to improve the connection strength. The assembly section 141 is sleeved outside the sleeve 120 and is clamped with the sleeve 120, so that the outer guide pipe 140 is detachably connected with the sleeve 120, the disassembly and the assembly are convenient, and the tightness of the communication between the oil outlet channel 160 and the oil inlet channel 150 can be ensured. The needle 130 is simultaneously arranged in the connecting section 142 and the necking section 143, the first positioning ribs 131 are matched with the connecting section 142, and the first positioning ribs 131 are used for limiting the connecting section 142, so that the whole outer catheter 140 is limited, and the centering accuracy of the outer catheter 140 and the needle 130 is ensured.
The needle 130 includes a mating segment 133, a coupling segment 134, and a tapered segment 135, which are connected in sequence. In this embodiment, the mating segment 133, the coupling segment 134 and the tapered segment 135 are integrally formed to enhance the connection strength. The matching section 133 is sleeved outside the needle tube 110 and is clamped with the needle tube 110, so that the needle 130 and the needle tube 110 can be detachably connected, the disassembly and the assembly are convenient, and the tightness of the communication between the needle 130 and the needle tube 110 can be ensured. The first positioning ribs 131 are simultaneously arranged on the matching section 133 and the connecting section 134, the tapered section 135 is partially extended into the necking section 143, the tapered section 135 is used for gradually shrinking the cross section area of the original solution, and the tapered section 135 and the necking section 143 are jointly used for gradually shrinking the cross section area of the flushing oil so as to ensure the outflow stability of the original solution and the flushing oil.
Preferably, a second positioning rib 144 is provided in the reduced section 143. The second positioning ribs 144 extend along the length direction of the outer catheter 140, and the second positioning ribs 144 are used for limiting the tapered section 135 when the outer catheter 140 is installed, so that the outer catheter 140 is displaced under the reaction force, the position of the outer catheter 140 is finely adjusted again, the centering accuracy of the outer catheter 140 and the needle 130 is further improved, the coaxial sheath flow effect of the original solution and the flushing oil is further improved, and uniform dripping of the water-in-oil micro-droplets is ensured.
Preferably, the inside of the necking section 143 is provided with a necking inner wall 1431 and a straight cylinder inner wall 1432 in sequence. The flushing oil in the oil outlet channel 160 sequentially passes through the necking inner wall 1431 and the straight cylinder inner wall 1432 in the outflow process, wherein the necking inner wall 1431 is used for gradually shrinking the cross-sectional area of the flushing oil so that the flushing oil can stably and uniformly flow out of the straight cylinder inner wall 1432. Specifically, the second positioning rib 144 is disposed on the inner wall 1431 of the throat, the second positioning rib 144 is matched with the tapered section 135, and the second positioning rib 144 is used for limiting the tapered section 135, so that the whole outer catheter 140 is limited under the reaction force, and the centering accuracy of the outer catheter 140 and the needle 130 is ensured.
Further, the end of the tapered section 135 remote from the coupling section 134 is provided with a capillary 136 extending. The capillary 136 is a straight tube, the capillary 136 extends into the inner wall 1432 of the straight tube, and is arranged at a coaxial interval with the inner wall 1432 of the straight tube, the capillary 136 is used for the original solution to flow out, the inner wall 1432 of the straight tube is used for the flushing oil to flow out, so that the original solution and the flushing oil generate coaxial sheath flow, when the acting force of the flushing oil is larger than the surface tension of the tiny liquid drops formed by the original solution, the tiny liquid drops fall off from the free ends of the capillary 136, and water-in-oil tiny liquid drops are formed one by one. Specifically, in the process of matching the second positioning rib 144 with the tapered section 135, on one hand, the second positioning rib 144 can limit the tapered section 135 so as to perform high-precision fine adjustment on the position of the outer catheter 140, further improve the centering precision of the outer catheter 140 and the needle 130, and on the other hand, the second positioning rib 144 can effectively protect the capillary 136 and improve the coaxiality of the capillary 136 and the straight cylinder inner wall 1432, thereby improving the coaxial sheath flow effect of the original solution and the flushing oil and ensuring that the water-in-oil micro-droplets uniformly drop out.
Specifically, the number of the second positioning ribs 144 is multiple, the second positioning ribs 144 are distributed in an annular array, a second gap 146 is formed between two adjacent second positioning ribs 144, the second gap 146 is used for allowing flushing oil to circulate, so that the second positioning ribs 144 are prevented from affecting the flow of the flushing oil in the oil outlet channel 160, and the second gaps 146 act together to ensure that the flow rate of the flushing oil can meet the requirement of dropping liquid.
Preferably, a limiting table 147 is disposed in the connecting section 142, and a step 137 is disposed at an end of the mating section 133 away from the coupling section 134. The step portion 137 is propped against the limiting table 147, and the limiting table 147 can limit the step portion 137 so as to limit the whole needle 130, prevent the needle 130 from being separated from the needle tube 110, and ensure the stability of the clamping connection between the needle 130 and the needle tube 110. Specifically, the limiting table 147 and the step portion 137 are all in annular arrangement, and the inner diameter of the limiting table 147 is smaller than the outer diameter of the step portion 137, so that the limiting table 147 can effectively support and limit the step portion 137, and the limiting effect is improved.
Further, the needle 130 is integrally disposed in the oil outlet channel 160, the stepped portion 137 is provided with an oil leakage hole 138, the oil leakage hole 138 is staggered with the first positioning rib 131 and is communicated with the first gap 132, and the oil leakage hole 138 is used for allowing flushing oil to flow through, so that the flushing oil can smoothly flow in the oil outlet channel 160, and the influence of the cooperation of the limiting table 147 and the stepped portion 137 on the flow of the flushing oil is avoided. Specifically, the number of the oil leakage holes 138 is multiple, the number of the oil leakage holes 138 is the same as the number of the first gaps 132, the oil leakage holes 138 are annularly arranged on the step portion 137, and the oil leakage holes 138 act together to simultaneously allow the flushing oil to pass through, so that the flow rate of the flushing oil flowing out of the oil outlet channel 160 is ensured to meet the requirement.
It should be noted that, the outer conduit 140 is disposed on the carrier plate 400, the carrier plate 400 is provided with a through hole 410, and the outer conduit 140 extends into the through hole 410. Specifically, the outer conduit 140 is provided with a hanging ring 148, the hanging ring 148 is annular, the hanging ring 148 is disposed at one end of the assembly section 141 far away from the connection section 142, the diameter of the through hole 410 is larger than the outer diameter of the assembly section 141 and smaller than the outer diameter of the hanging ring 148, and the outer conduit 140 can be hung on the bearing disc 400 through the hanging ring 148. Since the diameter of the through hole 410 is larger than the outer diameter of the mounting section 141, the outer guide tube 140 can be radially displaced by a certain amount in the through hole 410 to achieve fine adjustment of the position of the outer guide tube 140 and improve the centering accuracy of the outer guide tube 140 and the needle 130.
In the process of installing the outer catheter 140, firstly, the position of the needle 130 corresponds to the position of the outer catheter 140 on the bearing disc 400, then the needle tube 110, the sleeve 120 and the needle 130 are controlled to synchronously descend along the vertical direction so that the needle 130 stretches into the outer catheter 140, in the process, the first positioning ribs 131 on the outer wall of the needle 130 perform preliminary limiting on the outer catheter 140 to realize preliminary position fine adjustment of the outer catheter 140, then the needle tube 110, the sleeve 120 and the needle 130 are controlled to continuously descend along the vertical direction so that the second positioning ribs 144 in the necking section 143 are matched with the tapering section 135, so that the outer catheter 140 is limited again under the reactive force to realize the fine adjustment of the position of the outer catheter 140, and when the needle 130 moves to the limit position, the clamping of the outer catheter 140 and the sleeve 120 is completed, and the outer catheter 140 is installed.
According to the needle positioning mechanism 100 provided by the embodiment of the utility model, the sleeve 120 is connected with the needle tube 110 and sleeved outside the needle tube 110, an oil inlet channel 150 is formed between the sleeve 120 and the needle tube 110, the needle 130 is arranged at the end part of the needle tube 110, the outer guide tube 140 is arranged at the end part of the sleeve 120 and sleeved outside the needle 130, an oil outlet channel 160 is formed between the outer guide tube 140 and the needle 130, the oil outlet channel 160 is communicated with the oil inlet channel 150, a first positioning rib 131 is arranged on the outer wall of the needle 130, the first positioning rib 131 extends along the length direction of the needle 130, and the first positioning rib 131 is used for limiting the outer guide tube 140 when the outer guide tube 140 is arranged. Compared with the prior art, the needle positioning mechanism 100 provided by the utility model adopts the first positioning ribs 131 arranged on the outer wall of the needle 130 and the outer guide pipe 140 matched with the first positioning ribs 131, so that the centering precision of the outer guide pipe 140 and the needle 130 can be improved, the same proportion of the original solution in each water-in-oil micro-droplet is ensured, the uniformity of the water-in-oil micro-droplet is improved, and the nucleic acid detection precision is improved. The micro-droplet generation device 10 has high generation efficiency and good generation effect.
The above is only a preferred embodiment of the present utility model, and is not intended to limit the present utility model, but various modifications and variations can be made to the present utility model by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.
Claims (10)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202520025958.XU CN223832352U (en) | 2025-01-06 | 2025-01-06 | Needle positioning mechanism and micro-droplet generation device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202520025958.XU CN223832352U (en) | 2025-01-06 | 2025-01-06 | Needle positioning mechanism and micro-droplet generation device |
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| Publication Number | Publication Date |
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| CN223832352U true CN223832352U (en) | 2026-01-27 |
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| CN202520025958.XU Active CN223832352U (en) | 2025-01-06 | 2025-01-06 | Needle positioning mechanism and micro-droplet generation device |
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| CN (1) | CN223832352U (en) |
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