CN223602534U - An ultrafiltration centrifuge tube - Google Patents

Abstract

The utility model relates to the technical field of centrifuge tubes, in particular to an ultrafiltration centrifuge tube which can be applied to a centrifuge and comprises an outer tube, wherein a plug cover is connected to an opening at the top of the outer tube through a connecting part, an inner tube is inserted from the opening of the outer tube at the top end or the bottom end and stays in the outer tube, the inner tube filters and concentrates samples filled in the inner tube, the bottom end of the inner tube is provided with a dead volume chamber, a supporting structure is arranged between the inner tube and the outer tube, a liquid retaining cavity is arranged between the bottom end of the inner tube and the bottom end of the outer tube, and the concentrated samples are deposited in the liquid retaining cavity. In the utility model, the top end and the bottom end of the inner tube can be respectively inserted into the outer tube, namely, the inner tube is inserted positively and reversely. The outer tube is designed with a support structure for supporting the inner tube when the inner tube is inserted, and the inner tube is designed with a 'dead volume chamber' for preventing excessive centrifugation from drying out the sample, resulting in loss of the sample.

Description

Ultrafiltration centrifuge tube

Technical Field

The utility model relates to the technical field of centrifuge tubes, in particular to an ultrafiltration centrifuge tube.

Background

Ultrafiltration centrifuge tubes are a common medical device that is used mainly for the concentration and desalting of some liquid samples containing proteins. The inner tube structure of the existing ultrafiltration centrifuge tube mainly has three types:

The first structure is to set up filtration in the centrifuging tube, and filtration includes filtration membrane and the milipore filter that is located below the filtration membrane, and typical ultrafiltration tube structure that is used for exosome to draw as disclosed in the grant publication number CN219615282U, this kind of structure sample pass through membrane filtration, reach filtration and concentration effect, but the problem of this structure has the area of membrane little, influences filtration efficiency, does not have the design of dead volume, and the sample excessively centrifugation can lead to the sample to dry, sample loss.

The second structure is to fix the membrane in the designed square frame, and the membrane is sleeved in the centrifuge tube through the square frame, so that the filtering effect is achieved under the action of centrifugal force. The disadvantage of this construction is that the centrifugal force is outward and the membrane and the frame are bonded together, in contrast to the direction of the centrifugal force, the greater the centrifugal force the greater the risk of leakage

The third structure is an ultrasonic welding structure, wherein the ultrafiltration membrane is welded on the inner wall of the centrifuge tube, the ultrafiltration membrane and the inner wall of the centrifuge tube are bonded after being in a molten state by ultrasonic welding, and the ultrafiltration membrane is in an open bag shape after being bonded. And because the ultrafiltration membrane is extremely fragile, vibration can cause invisible damage to the ultrafiltration membrane, so that the actual product filtering effect can be affected.

Disclosure of utility model

The utility model aims to provide an ultrafiltration centrifuge tube so as to solve the problems in the background art.

In order to achieve the above purpose, the present utility model provides the following technical solutions:

an ultrafiltration centrifuge tube, the ultrafiltration centrifuge tube being applicable in a centrifuge and comprising:

The opening at the top of the outer tube is connected with a plug cover through a connecting part;

The inner tube is inserted from the opening of the outer tube at the top end or the bottom end and stays in the outer tube;

The inner tube filters and concentrates the sample filled in the inner tube, and the bottom end of the inner tube is provided with a dead volume chamber;

A supporting structure is arranged between the inner tube and the outer tube, so that a liquid retaining cavity is arranged between the bottom end of the inner tube and the bottom end of the outer tube, and a filtered and concentrated sample is deposited in the liquid retaining cavity;

The liquid retention cavity is internally provided with an anti-reflux part for preventing the sample deposited in the liquid retention cavity from reflux.

Preferably, the dead volume chamber is one tenth to one eighth of the volume of the inner tube.

Preferably, the inner tube comprises a filtering matrix and a filter membrane, the filter membrane is arranged on the filtering matrix, the filtering matrix comprises a sample injection opening arranged at the first end of the filtering matrix, a filter cavity communicated with the sample injection opening, and a flow guide hole arranged on the filtering matrix and corresponding to the second end of the filtering matrix, and the dead volume chamber is positioned at the position of the filter cavity close to the second end.

Preferably, the side of the filtering matrix, which is close to the filtering cavity, is provided with a diversion channel, the diversion hole penetrates through the filtering matrix and is communicated with the diversion channel, and the filter membrane is arranged between the filtering matrix and the filtering cavity and covers the diversion channel and the diversion hole for filtering samples in the filtering cavity.

Preferably, the support structure comprises an annular step in the outer tube, the bottom of the inner tube having a protrusion, the annular step being for supporting the protrusion.

Preferably, the diameter of the inner tube is not smaller than the smallest inner diameter of the annular step.

Preferably, the annular step is provided with an upward convex ring, and the top end and the bottom end of the inner tube are provided with grooves which are matched and inserted with the convex ring.

Preferably, the anti-reflux part comprises a baffle plate, the edge of the baffle plate is fixed on the inner wall of the liquid retaining cavity in a sealing way, the middle part of the baffle plate is provided with a cylindrical part with a flow passage, and the cylindrical part is provided with a penetration hole.

Preferably, the upper part of the baffle plate is provided with an opening, the opening is provided with an upward inclined sliding part, the sliding part is fixed with the inner wall of the liquid retaining cavity in a sealing way, and a cavity is arranged below the opening.

Preferably, the baffle is in an omega shape, the inner diameter of the opening is smaller than that of the cavity, and the top of the cylindrical part is close to the opening.

Compared with the prior art, the utility model has the beneficial effects that:

In the utility model, the top end and the bottom end of the inner tube can be respectively inserted into the outer tube, namely, the inner tube is inserted positively and reversely. The inner tube is designed with a "dead volume chamber" of about 40ul, preventing excessive centrifugation from drying out the sample, resulting in loss of sample. The outer tube design bearing structure for support the inner tube when the inner tube inserts, the inner tube can be inverted the centrifugation, and in the practical application process, the sample volume can be very little after the centrifugation, is difficult to collect, leads to after the centrifugation once more, and concentrated sample can be collected in the outer tube, conveniently collects, and the filter effect is relatively better.

According to the utility model, the anti-reflux part, especially the inner diameter of the opening is smaller than the inner diameter of the cavity, and the top of the columnar part is close to the opening, so that the baffle can prevent/minimize the reflux of filtered liquid into the liquid-retaining cavity or the inner tube above the baffle when the ultrafiltration centrifuge tube is used, and the influence on the filtering effect of the ultrafiltration centrifuge tube is prevented.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings that are needed in the description of the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present utility model, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic view showing the structure of an inner tube inserted into an outer tube (being inserted) at the bottom end thereof according to an exemplary embodiment of the present utility model;

FIG. 2 is a vertical view of FIG. 1 in accordance with the present utility model;

FIG. 3 is a top view of an exemplary embodiment of the present utility model;

FIG. 4 is a left side view of FIG. 1 in accordance with the present utility model;

FIG. 5 is a schematic view showing a state of preparation in which the bottom end of the inner tube is inserted into the outer tube according to an exemplary embodiment of the present utility model;

FIG. 6 is a schematic illustration of the completed insert of FIG. 5 in accordance with the present utility model;

FIG. 7 is a schematic view showing a ready state in which the tip of the inner tube is inserted into the outer tube (upside down insertion) according to an exemplary embodiment of the present utility model;

FIG. 8 is a schematic illustration of the insertion of FIG. 7 in the middle of the present utility model;

FIG. 9 is a schematic view of the structure of an outer tube according to an exemplary embodiment of the present utility model;

FIG. 10 is a schematic view of the structure of an inner tube according to an exemplary embodiment of the present utility model;

FIG. 11 is a schematic view of the cross-sectional structure A-A of FIG. 10 in accordance with the present utility model;

FIG. 12 is a side view of a filter substrate according to an exemplary embodiment of the present utility model;

FIG. 13 is a schematic view of the cross-sectional structure B-B of FIG. 10 in accordance with the present utility model;

FIG. 14 is a top view of FIG. 12 in accordance with the present utility model;

FIG. 15 is a vertical view of FIG. 12 in accordance with the present utility model;

FIG. 16 is a schematic view of another construction of an outer tube according to an exemplary embodiment of the present utility model;

FIG. 17 is a schematic view of another construction of an inner tube according to an exemplary embodiment of the present utility model;

FIG. 18 is a schematic view of the structure of FIG. 16 and the structure of FIG. 17 adapted for insertion in accordance with the present utility model;

Fig. 19 is a schematic view of the structure of the anti-reflux unit of the present utility model.

In the figure, the device comprises a 1-outer tube, a 2-inner tube, a 3-filter membrane, a 4-annular step, a 5-plug cover, a 6-connecting part, a 7-dead volume chamber, an 8-diversion hole, a 9-bulge, a 10-filter matrix, an 11-liquid retention cavity, a 12-sample injection opening, a 13-filter cavity, a 14-diversion channel, a 15-bulge loop, a 16-groove, a 17-backflow prevention part, a 18-baffle, a 19-column part, a 20-opening, a 21-cavity, a 22-permeation hole, a 23-sliding part and a 24-runner.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. The following description of at least one exemplary embodiment is merely exemplary in nature and is in no way intended to limit the utility model, its application, or uses. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the present application. As used herein, the singular is also intended to include the plural unless the context clearly indicates otherwise, and furthermore, it is to be understood that the terms "comprises" and/or "comprising" when used in this specification are taken to specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof.

The relative arrangement of the components and steps, numerical expressions and numerical values set forth in these embodiments do not limit the scope of the present utility model unless it is specifically stated otherwise. Meanwhile, it should be understood that the sizes of the respective parts shown in the drawings are not drawn in actual scale for convenience of description. Techniques, methods, and apparatus known to one of ordinary skill in the relevant art may not be discussed in detail, but should be considered part of the specification where appropriate. In all examples shown and discussed herein, any specific values should be construed as merely illustrative, and not a limitation. Thus, other examples of the exemplary embodiments may have different values. It should be noted that like reference numerals and letters refer to like items in the following figures, and thus once an item is defined in one figure, no further discussion thereof is necessary in subsequent figures.

In the description of the present utility model, it should be understood that the azimuth or positional relationships indicated by the azimuth terms such as "front, rear, upper, lower, left, right", "lateral, vertical, horizontal", and "top, bottom", etc., are generally based on the azimuth or positional relationships shown in the drawings, and are merely for convenience of describing the present utility model and simplifying the description, and these azimuth terms do not indicate and imply that the apparatus or elements referred to must have a specific azimuth or be constructed and operated in a specific azimuth, and thus should not be construed as limiting the scope of the present utility model, and the azimuth terms "inside and outside" refer to inside and outside with respect to the outline of each component itself.

Spatially relative terms, such as "above," "upper" and "upper surface," "above" and the like, may be used herein for ease of description to describe one device or feature's spatial relationship to another device or feature as illustrated in the figures. It will be understood that the spatially relative terms are intended to encompass different orientations in use or operation in addition to the orientation depicted in the figures. For example, if the device in the figures is turned over, elements described as "above" or "over" other devices or structures would then be oriented "below" or "beneath" the other devices or structures. Thus, the process is carried out, the exemplary term "above" may be included. Upper and lower. Two orientations below. The device may also be positioned in other different ways (rotated 90 degrees or at other orientations) and the spatially relative descriptors used herein interpreted accordingly.

In addition, the terms "first", "second", etc. are used to define the components, and are only for convenience of distinguishing the corresponding components, and the terms have no special meaning unless otherwise stated, and therefore should not be construed as limiting the scope of the present utility model.

Referring to fig. 1 to 19, the present utility model provides a technical solution:

An ultrafiltration centrifuge tube which can be used in a centrifuge for separating biological substances such as abzymes, nucleic acids and proteins for the purpose of concentration, desalting, purification and fractionation, such as separation and collection of concentrated substances (hereinafter described in detail as concentrate) such as concentrated urine, serum, plasma and cerebrospinal fluid, and which comprises:

The outer tube 1, the opening at the top of the outer tube 1 is connected with a plug cover 5 through a connecting part 6;

An inner tube 2 inserted from the opening of the outer tube 1 at the top end or the bottom end, and staying inserted in the outer tube 1;

The inner tube 2 filters and concentrates the sample filled in the inner tube 2, and the bottom end of the inner tube 2 is provided with a dead volume chamber 7;

A supporting structure is arranged between the inner tube 2 and the outer tube 1, so that a liquid retaining cavity 11 is arranged between the bottom end of the inner tube 2 and the bottom end of the outer tube 1, and a concentrated sample is deposited in the liquid retaining cavity 11;

The retention chamber 11 has therein an anti-reflux portion 17 for preventing reflux of the sample deposited in the retention chamber 11.

In a specific embodiment, as shown in fig. 1, the dead volume chamber 7 is one tenth to one eighth of the volume of the inner tube 2. The inner tube 2 is designed with a "dead volume chamber" of about 40ul of dead volume chamber 7 to prevent excessive centrifugation from drying out the sample, resulting in loss of sample.

In a specific embodiment, as shown in fig. 4 and fig. 10 to fig. 15, the inner tube 2 includes a filter substrate 10 and a filter membrane 3, the filter membrane 3 is disposed on the filter substrate 10, the filter substrate 10 includes a sample inlet opening 12 disposed at a first end of the filter substrate 10, a filter cavity 13 communicating with the sample inlet opening 12, and a flow guiding hole 8 disposed on the filter substrate 10 and corresponding to a second end of the filter substrate, the dead volume chamber 7 is disposed at a position of the filter cavity 13 near the second end, a flow guiding channel 14 is disposed on a side of the filter substrate 10 near the filter cavity 13, the flow guiding hole 8 penetrates the filter substrate 10 and communicates with the flow guiding channel 14, and the filter membrane 3 is disposed between the filter substrate 10 and the filter cavity 13 and covers the flow guiding channel 14 and the flow guiding hole 8, so as to filter a sample in the filter cavity 13.

A filter 3 such as an ultrafiltration membrane. The volume between the hole 8 at the bottom of the filter membrane 3 and the bottom of the inner tube 2 is the dead volume chamber 7. The sample added to the inner tube 2 passes through the filter membrane 3 during centrifugation, and is used for trapping small particle molecules added to the sample in the inner tube 2. The sample passes through the filter membrane 3 to achieve the effects of filtration and concentration.

The inner tube 2 is used for filtering and centrifuging the sample liquid to be filtered, and the (liquid-retaining cavity 11 of the) outer tube 1 is used for collecting filtered filtrate or concentrated liquid (such as concentrated urine). A sample (or sample fluid) can enter the filter chamber 13 from the sample inlet opening 12 and be filtered through the filter membrane 13 to form a filtrate, which can be discharged from the filter chamber 13 through the flow guiding hole 8, and unfiltered sample fluid (hereinafter described as concentrated fluid) remains in the dead volume chamber 7. The filter base 10 is a three-dimensional housing, preferably a hollow cylinder, with one end open and the other end closed.

When in use, the inner tube 2 extends into the outer tube 1 from the opening of the outer tube 1, when sample liquid is separated and filtered, the sample liquid is filtered by the filter membrane 3, filtrate can be drained into the outer tube 1 from the diversion holes 8, concentrated solution is gathered in the dead volume chamber 7, the inner tube 2 is detached from the outer tube 1, and the concentrated solution in the dead volume chamber 7 is transferred to other experimental containers (such as test tubes) for storage, so that different experimental purposes are realized. And the plug cover 5 is used for sealing and storing, so that waste and pollution are avoided. The opening part of the outer tube 1 can be provided with external threads which are in threaded connection with a cover body provided with internal threads, or the opening part of the outer tube 1 is provided with a plug cover 5, and sealing preservation is realized by plugging the plug cover into the opening.

Sample liquid can be filtered through the filter membrane 3 and then drained to the flow guide hole 8 from the flow guide channel 14, and then discharged to the liquid retaining cavity 11 through the flow guide hole 8, so that the collection of filtrate is realized, the flow guide channel 14 and the flow guide hole 8 are arranged, the filtrate can be drained, and the phenomenon that liquid leakage or incomplete filtrate collection is avoided, and the effect of filtration and separation is influenced.

The diversion channel 14 comprises a plurality of diversion trenches which are uniformly distributed on the side surface of the filtering matrix 10, which is close to the filter membrane 3, and extend along the axial direction of the filtering matrix 10 (the direction of connecting the first end center point and the second end center point), and the diversion trenches are at least communicated with one diversion hole 8 so as to drain the centrifugally filtered filtrate into the liquid reserving cavity 11, thereby avoiding liquid leakage.

Preferably, the diversion holes 8 are arranged in one-to-one correspondence with the diversion trenches, as shown in fig. 13, 4 diversion holes 8 are arranged on each side surface of the diversion trench.

In a specific embodiment, as shown in fig. 2 and 8 to 9, the outer tube 1 is designed as a supporting structure, the supporting structure comprises an annular step 4 in the outer tube 1, the bottom of the inner tube 2 is provided with a protrusion 9, and the annular step 4 is used for supporting the protrusion 9. After the positive insertion is completed, the protrusion 9 at the bottom end of the inner tube 2 is located at the annular step 4, and the annular step 4 supports the protrusion 9. At this time, the completion of the inner tube 2 is explained is inserted into the outer tube 1.

In a specific embodiment, as shown in fig. 5, the diameter of the inner tube 2 is not smaller than the minimum inner diameter of the annular step 4. In the case of the inverted insertion, the top end of the inner tube 2 is inserted into the outer tube 1, and after the top end of the inner tube 2 is in contact with the annular step 4, the annular step 4 supports the top end of the inner tube 2, at this time, it is explained that the inverted insertion of the inner tube 2 into the outer tube 1 is completed.

In the present utility model, the top and bottom ends of the inner tube 2 can be inserted into the outer tube 1, i.e., inserted in the normal direction and inserted in the inverted direction, respectively. After the insertion, the opening of the outer tube 1 is covered by the plug cover 5, so that the inner tube 1 is pressed in the outer tube 1 to form a centrifuge tube, and the centrifuge tube can be placed in a centrifuge for centrifugation.

In the utility model, as shown in fig. 16-18, the annular step 4 is provided with an upward convex ring 15, and the top end and the bottom end of the inner tube 2 are provided with grooves 16 which are matched and inserted with the convex ring 15. Through bulge loop 15 and recess 16 adaptation cartridge, like this after inner tube 2 just inserts or inverts the completion of inserting, utilize bulge loop 15 and recess 16 frictional force each other, can realize the two and fix relatively fast, like this when centrifuging, can reduce the two relative rotation to can assist plug 5 to the mutual fixation between inner tube 2 and the outer tube 1.

The utility model is characterized in that the ultrafiltration tube is provided with a dead volume chamber, in particular, the inside/bottom of the inner tube 2 is reserved with a dead volume chamber, the dead volume chamber 7 is about 40ul, and the sample is dried due to excessive centrifugation, so that the sample is lost. As shown in fig. 1, the dead volume chamber 7 is the volume/volume of the portion below the deflector hole 8 (the bottom of the inner tube 2). As shown in fig. 5 to 8, the outer tube 1 is designed into a supporting structure, the inner tube 2 can be inverted and centrifuged, in the practical application process, the sample size after centrifugation is very small and is difficult to collect, so that after re-centrifugation, the concentrated sample can be collected in (the liquid-retaining cavity 11 of) the outer tube 1, and collection is convenient. The encapsulation technology is that the ultrafiltration centrifuge tube product is integrally formed through the structural design of a mould, and the plastic piece (the inner tube 2) welded with the filter membrane 3 is encapsulated into an integral structure through thermoplastic glue.

The utility model, when in use, comprises the following steps:

1. Inserting the inner tube 2 into the outer tube 1 to form a centrifuge tube, and then adding a liquid sample into the inner tube 2;

2. Placing the centrifugal tube into a centrifugal machine for centrifugation, wherein 12000g of the centrifugal tube is centrifuged for 20mins at room temperature;

3. Taking out the centrifuge tube, replacing the centrifuge tube with a new outer tube 1, at this time, a small amount of concentrated liquid is arranged at the bottom of the inner tube 2, reversely inserting the inner tube 2 into the outer tube 1, centrifuging again, and collecting the concentrated liquid at the bottom of the outer tube 1, so that the subsequent detection is facilitated.

The filter membrane 3 is used for filtration, and is welded at the side recess 10 of the inner tube 2, and the sample concentrated by the filter membrane 3 can be collected in the outer tube 1.

In one embodiment, as shown in fig. 18 to 19, an anti-reflux portion 17 for preventing reflux of the sample deposited in the liquid-retaining chamber 11 is provided in the liquid-retaining chamber 11. The anti-reflux part 17 is positioned at a point below the annular step 4 of the supporting structure, has a certain distance with the top end and the bottom end of the inner tube 2, prevents touching the dead volume chamber 7, and comprises a baffle plate 18, the edge of the baffle plate 18 is fixed on the inner wall of the liquid retaining cavity 11 in a sealing way, the middle part of the baffle plate 18 is provided with a cylindrical part 19 with a flow passage 24, and the cylindrical part 19 is provided with a penetration hole 22. The upper opening 20 of the baffle plate 18 is provided with an upward inclined sliding part 23 at the opening 20, the sliding part 23 is fixed with the inner wall of the liquid retaining cavity 11 in a sealing way, and a cavity 21 is arranged below the opening 20. The baffle 18 is in an omega shape as a whole, the inner diameter of the opening 20 is smaller than that of the cavity 21, and the top of the cylindrical part 19 is close to the opening 20.

The sliding part 23 and the column part 19 are both part of the baffle plate 18, and the sliding part 23 is obliquely arranged, namely, the top part is inclined downwards, so that the filtered liquid formed by filtering through the filter membrane 13 can quickly slide into the cavity 21. The filtered liquid enters the cavity 21 and falls into the liquid retaining cavity 11 below the baffle plate 18 through the penetrating holes 22 and the flow channels 24 in sequence during centrifugation.

Through preventing backward flow portion 17, especially opening 20 internal diameter is less than cavity 21 internal diameter and cylindricality portion 19 top is close to opening 20 department, can be when the ultrafiltration centrifuging tube uses, the separation blade 18 can stop/minimize the liquid backward flow after filtering in the liquid cavity 11 or the inner tube 2 that stay that lie in separation blade 18 top, prevent to influence the filter effect of ultrafiltration centrifuging tube.

The sliding part 23 is inclined, and the inner diameter of the opening 20 is smaller than that of the cavity 21, so that the filtrate below the filter membrane 13 is quickly transferred into the liquid retaining cavity 11 below the baffle plate 18, and the efficiency of the dead volume chamber 7 on the concentration of the filtrate is improved.

The baffle 18 can be made of medical silica gel or PET, or is consistent with the outer tube 1 and the inner tube 2, and can be determined according to actual use conditions.

The present utility model, not described in part, is prior art.

The present utility model is not limited to the above-mentioned embodiments, and any person skilled in the art, based on the technical solution of the present utility model and the inventive concept thereof, can be replaced or changed within the scope of the present utility model.

Claims (10)

1. An ultrafiltration centrifuge tube, wherein the ultrafiltration centrifuge tube is applicable in a centrifuge and comprises:

The plug cover (5) is connected to the opening at the top of the outer tube (1) through a connecting part (6);

An inner tube (2), wherein the inner tube (2) is inserted from the opening of the outer tube (1) at the top end or the bottom end and stays in the outer tube (1);

The inner tube (2) filters and concentrates the sample filled in the inner tube (2), and the bottom end of the inner tube (2) is provided with a dead volume chamber (7);

a supporting structure is arranged between the inner tube (2) and the outer tube (1), so that a liquid retaining cavity (11) is arranged between the bottom end of the inner tube (2) and the bottom end of the outer tube (1), and a filtered and concentrated sample is deposited in the liquid retaining cavity (11);

The liquid retention cavity (11) is internally provided with an anti-reflux part (17) for preventing the sample deposited in the liquid retention cavity (11) from reflux.

2. An ultrafiltration centrifuge tube according to claim 1, characterized in that the dead volume chamber (7) is one tenth to one eighth of the volume of the inner tube (2).

3. An ultrafiltration centrifuge tube according to claim 1, wherein the inner tube (2) comprises a filter matrix (10) and a filter membrane (3), the filter membrane (3) is arranged on the filter matrix (10), the filter matrix (10) comprises a sample introduction opening (12) arranged at a first end of the filter matrix (10), a filter cavity (13) communicated with the sample introduction opening (12), and a flow guide hole (8) arranged on the filter matrix (10) and at a second end corresponding to the first end, and the dead volume chamber (7) is arranged at the position of the filter cavity (13) close to the second end.

4. An ultrafiltration centrifuge tube according to claim 3, wherein the side of the filter matrix (10) adjacent to the filter cavity (13) is provided with a flow guide channel (14), the flow guide hole (8) penetrates the filter matrix (10) and is communicated with the flow guide channel (14), and the filter membrane (3) is arranged between the filter matrix (10) and the filter cavity (13) and covers the flow guide channel (14) and the flow guide hole (8) for filtering the sample in the filter cavity (13).

5. An ultrafiltration centrifuge tube according to claim 3, characterized in that the support structure comprises an annular step (4) in the outer tube (1), the bottom of the inner tube (2) having a bulge (9), the annular step (4) being adapted to support the bulge (9).

6. An ultrafiltration centrifuge tube according to claim 5, wherein the diameter of the inner tube (2) is not smaller than the smallest inner diameter of the annular step (4).

7. An ultrafiltration centrifuge tube according to claim 5, characterized in that the annular step (4) has an upward bulge loop (15), and that the top and bottom ends of the inner tube (2) have grooves (16) which fit into the bulge loop (15).

8. An ultrafiltration centrifuge tube according to claim 1, wherein the anti-reflux portion (17) comprises a baffle (18), the edge of the baffle (18) is fixed on the inner wall of the liquid retaining cavity (11) in a sealing way, a cylindrical portion (19) with a flow passage (24) is arranged in the middle of the baffle (18), and a penetration hole (22) is formed in the cylindrical portion (19).

9. The ultrafiltration centrifuge tube according to claim 8, wherein the upper part of the baffle (18) is provided with an opening (20), an upward inclined sliding part (23) is arranged at the opening (20), the sliding part (23) is fixed with the inner wall of the liquid retaining cavity (11) in a sealing way, and a cavity (21) is arranged below the opening (20).

10. An ultrafiltration centrifuge tube according to claim 9, wherein the baffle (18) is integrally omega-shaped, the opening (20) has an inner diameter smaller than the inner diameter of the cavity (21), and the top of the cylindrical portion (19) is adjacent to the opening (20).