CN223516994U

CN223516994U - Centrifuge tube capable of rapidly purifying protein

Info

Publication number: CN223516994U
Application number: CN202423118661.2U
Authority: CN
Inventors: 张蕴文
Original assignee: Tianjin Gracie Technology Co ltd
Current assignee: Tianjin Gracie Technology Co ltd
Priority date: 2024-12-17
Filing date: 2024-12-17
Publication date: 2025-11-07
Anticipated expiration: 2034-12-17

Abstract

The utility model discloses a centrifuge tube capable of rapidly purifying proteins, which relates to the technical field of centrifuge tubes and comprises an upper tube body, wherein the lower end of the upper tube body is in threaded connection with a lower tube body, a filtering mechanism is arranged at the joint of the upper tube body and the lower tube body, a separation tube is arranged in the upper tube body, two symmetrical liquid through holes are formed in the lower end of the separation tube, a sealing part is arranged at the liquid through holes, a fixing module is arranged at the upper end of the separation tube, larger proteins are trapped in the lower tube body through the filtering mechanism, supernatant and smaller impurities can enter the upper tube body through the filtering mechanism, and the sealing part on the separation tube seals the supernatant and the smaller impurities in the separation tube, so that the supernatant and the larger proteins are separated, the subsequent further operation is facilitated, the separation effect is effectively improved, the working efficiency is improved, the separation tube can be rapidly disassembled and installed through the fixing module, and the supernatant is conveniently taken out.

Description

Centrifuge tube capable of rapidly purifying protein

Technical Field

The utility model relates to the technical field of centrifuge tubes, in particular to a centrifuge tube capable of rapidly purifying protein.

Background

Proteins are typically present in tissues or cells in complex mixtures, each type of cell containing thousands of different proteins. The general goal of protein separation and purification is to increase the purity of the product, the purification is required to separate the desired protein from the impurity protein with reasonable efficiency, speed, yield and purity, a centrifuge tube is required for protein separation, the biological sample suspension is put into the centrifuge tube for high-speed rotation, and the suspended tiny particles (such as organelles, precipitation of biological macromolecules and the like) are settled at a certain speed due to the huge centrifugal force effect, so that the suspended tiny particles are separated from the solution.

After purification is completed, the conventional centrifuge tube generally needs to manually transfer the supernatant separated after centrifugation, and then the lower layer macromolecular protein is poured, but the supernatant separated manually contains impurities easily, so that the subsequent further operation is inconvenient, the separation effect is poor, and the working efficiency is low.

Based on this, now provide a centrifuging tube that can purify protein fast, can eliminate the drawback that current device exists.

Disclosure of utility model

The utility model aims to provide a centrifuge tube capable of rapidly purifying protein, which solves the problems that impurities are easily contained in supernatant liquid obtained by manual separation, the subsequent further operation is inconvenient, the separation effect is poor and the working efficiency is low.

In order to achieve the above purpose, the present utility model provides the following technical solutions:

The centrifuge tube capable of rapidly purifying protein comprises an upper tube body, wherein the lower end of the upper tube body is in threaded connection with a lower tube body, a filtering mechanism is arranged at the joint of the upper tube body and the lower tube body, a separation tube is arranged in the upper tube body, two symmetrical liquid through holes are formed in the lower end of the separation tube, a sealing part is arranged at the liquid through holes, and a fixing module is arranged at the upper end of the separation tube.

Based on the technical scheme, the utility model also provides the following optional technical schemes:

In an alternative scheme, the filtering mechanism comprises a fixed circular ring, the inside of the fixed circular ring is fixedly connected with a separating die, the upper end of the separating die is provided with a fixing frame, the fixing frame is fixedly connected to the inner wall of the fixed circular ring, and the upper end of the fixing frame is fixedly connected with a fixing block.

In an alternative scheme, the sealing component comprises a rotating block, the rotating block is rotatably connected to the lower end of the separating tube, the outer end of the rotating block is fixedly connected with two symmetrically arranged baffles, the positions of the baffles correspond to the liquid through holes, and the lower end of the rotating block is provided with clamping grooves corresponding to the fixing blocks.

In an alternative scheme, the fixing module comprises four limiting blocks, the four limiting blocks are fixedly connected to the upper end of the separating tube, and limiting grooves corresponding to the limiting blocks are formed in the upper end of the upper tube body.

In an alternative scheme, the surface of the outer side wall of the separation tube is provided with an annular protruding block, one side of the protruding block, which is far away from the separation tube, is abutted against the inner wall of the upper tube body, and the protruding block is made of rubber.

In an alternative scheme, the upper end of the separation tube is fixedly connected with a sealing cover.

In an alternative, the upper end surface of the baffle plate completely covers the liquid through hole.

In an alternative scheme, the four limiting blocks are uniformly distributed on the separating tube.

Compared with the prior art, the utility model has the following beneficial effects:

According to the utility model, larger protein is trapped in the lower pipe body through the filtering mechanism, the supernatant and smaller impurities can enter the upper pipe body through the filtering mechanism, and the sealing component on the separating pipe seals the supernatant and the smaller impurities in the separating pipe, so that the supernatant and the larger protein are separated, the subsequent further operation is convenient, the separating effect is effectively improved, the working efficiency is improved, the separating pipe can be quickly disassembled and installed through the fixing module, the supernatant is conveniently taken out, and the practicability is strong.

Drawings

Fig. 1 is a schematic structural view of the present utility model.

Fig. 2 is a cross-sectional view of the present utility model.

Fig. 3 is a schematic structural diagram of a of the present utility model.

Fig. 4 is a schematic structural diagram of the B of the present utility model.

Fig. 5 is a schematic structural view of the separator tube of the present utility model.

Reference numerals are annotated as 100, an upper pipe body, 200, a lower pipe body, 300, a separation pipe, 301, a liquid through hole, 401, a fixed circular ring, 402, a separation mold, 403, a fixing frame, 404, a fixing block, 501, a rotating block, 502, a baffle, 503, a clamping groove, 601, a limiting block, 602, a limiting groove, 700, a protruding block, 800 and a sealing cover.

Detailed Description

The present utility model will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present utility model more apparent.

In one embodiment, as shown in fig. 1-5, a centrifuge tube capable of rapidly purifying protein comprises an upper tube body 100, wherein the lower end of the upper tube body 100 is in threaded connection with a lower tube body 200, a filtering mechanism is arranged at the joint of the upper tube body 100 and the lower tube body 200, a separation tube 300 is arranged in the upper tube body 100, two symmetrical liquid through holes 301 are arranged at the lower end of the separation tube 300, a sealing component is arranged at the liquid through holes 301, a fixing module is arranged at the upper end of the separation tube 300, when the device is used, firstly liquid to be purified is put into the lower tube body 200, the upper tube body 100 is rotated in a threaded connection mode, the lower end of the upper tube body 100 is fixedly connected with the upper end of the lower tube body 200, the separation tube 300 is fixedly connected in the upper tube body 100 through the fixing module, the separation tube 300 is put into a centrifuge for centrifugation, the protein with larger mass is blocked in the lower tube body 200 through the filtering mechanism, impurities with smaller mass enter the separation tube 300 in the upper tube body 100 through the filtering mechanism, the inside the separation tube 300 is automatically taken out, the separation tube 300 is automatically removed through the sealing component, and the lower end of the separation tube 300 is rapidly separated, and thus the protein is rapidly purified.

In this embodiment, as shown in fig. 4, the filtering mechanism includes a fixed ring 401, the inside of the fixed ring 401 is fixedly connected with a separation die 402, the upper end of the separation die 402 is provided with a fixing frame 403, the fixing frame 403 is fixedly connected with the inner wall of the fixed ring 401, the upper end of the fixing frame 403 is fixedly connected with a fixing block 404, the separation die 402 is fixedly connected with the connection position of the lower end of the upper pipe body 100 and the lower pipe body 200 through the fixed ring 401, proteins with different mass sizes are separated and purified through the separation die 402, proteins with larger mass can be blocked at the lower end of the separation die 402, proteins with smaller mass can pass through the separation die 402 and enter the separation pipe 300 through a liquid through hole 301, and the device can be used for quickly separating and purifying proteins, and can also be suitable for purifying different kinds of proteins by replacing the separation die 402 with different apertures according to the proteins with different required sizes.

In one embodiment, as shown in fig. 4 and fig. 5, the sealing member includes a rotating block 501, the rotating block 501 is rotatably connected to the lower end of the separation tube 300, the outer end of the rotating block 501 is fixedly connected to two symmetrically arranged baffles 502, the positions of the baffles 502 correspond to the liquid through holes 301, a clamping groove 503 corresponding to the fixed block 404 is provided at the lower end of the rotating block 501, when the separation tube 300 is inserted into the upper tube body 100, the clamping groove 503 is matched with the fixed block 404, the upper tube body 100 is rotated to drive the fixed block 404 to rotate, the fixed block 404 drives the rotating block 501 to rotate, the rotating block 501 drives the baffles 502 fixedly connected to both sides to rotate, thereby opening the liquid through holes 301, so that the liquid passing through the separation die 402 can enter the separation tube 300 through the liquid through holes 301, after separation, the upper tube body 100 is reversely rotated, the rotating block 501 is driven to rotate, the baffles 502 are reset to seal the liquid through holes 301, the liquid is sealed in the separation tube 300, and thus the protein is rapidly separated and purified.

In one embodiment, as shown in fig. 1 and fig. 3, the fixing module includes four limiting blocks 601, four limiting blocks 601 are fixedly connected to the upper end of the separating tube 300, a limiting groove 602 corresponding to the limiting block 601 is provided at the upper end of the upper tube body 100, when the separating tube 300 is inserted into the upper tube body 100, the limiting block 601 is clamped into the limiting groove 602, and along with rotation of the separating tube 300, the limiting block 601 and the limiting groove 602 cooperate to fix the separating tube 300, and when the separating tube 300 needs to be taken out, only the reverse rotation of the separating tube 300 is needed to take out.

In one embodiment, as shown in fig. 2, the outer side wall surface of the separation tube 300 is provided with an annular protrusion block 700, one side of the protrusion block 700 away from the separation tube 300 is abutted against the inner wall of the upper tube body 100, the protrusion block 700 is made of rubber, the annular rubber protrusion block 700 can seal between the separation tube 300 and the upper tube body 100, prevent leakage, and increase the stability of the separation tube 300, and prevent the separation tube 300 from shaking inside the upper tube body 100 during separation, thereby affecting the separation effect.

In one embodiment, as shown in fig. 2, the upper end of the separation tube 300 is fixedly connected to a sealing cap 800, so as to seal the upper end of the separation tube 300, and prevent the liquid from leaking during the separation process of the separation tube 300.

In one embodiment, as shown in fig. 5, the upper end surface of the baffle 502 completely covers the liquid through hole 301, and plugs the liquid through hole 301, so as to separate proteins with different qualities, and achieve protein purification.

In one embodiment, as shown in fig. 1, four limiting blocks 601 are uniformly distributed on the separating tube 300, so that the layout is stable, the stress is more uniform, the effective utilization of space is realized, and the stability of fixing the separating tube 300 is increased.

The embodiment discloses a centrifuging tube that can purify protein fast, wherein when using the device, liquid that will purify is put into earlier in lower body 200, rotate upper body 100 through threaded connection's mode, with the fixed connection of the lower extreme of upper body 100 and the upper end of lower body 200, insert separating tube 300 in upper body 100, at this moment fixed block 404 card goes into draw-in groove 503, stopper 601 card goes into limit groove 602, rotate upper body 100, drive fixed block 404 and rotate, fixed block 404 drives rotatory piece 501 and rotates, rotatory piece 501 drives both sides fixed connection's baffle 502 rotation, thereby open liquid hole 301, stopper 601 and limit groove 602 mutually support and fix separating tube 300, sealed lid 800 seals the upper end of separating tube 300, prevent to take place to leak, can increase the stability of separating tube 300, prevent to take place in the process of separation, influence the effect of separation, put into centrifugal centrifuge in, separation mould 402 through fixed ring 401 fixed connection's upper body 100 lower end and the lower body 200 can be passed through the separation mould and the large-size of separating tube 402 and can be carried out the purification device with the different quality of protein purification, the same kind can be carried out to the separation mould 402 through the separation mould is big and the purification of protein that can be passed through the lower end of separating mould 402, the purification is different quality of separating device is realized.

The foregoing is merely illustrative of the present application, and the present application is not limited thereto, and any person skilled in the art will readily recognize that variations or substitutions are within the scope of the present application. Therefore, the protection scope of the application is subject to the protection scope of the claims.

Claims

1. A centrifuge tube for rapid protein purification, comprising: an upper tube body (100), characterized in that the lower end of the upper tube body (100) is threadedly connected to a lower tube body (200), a filtration mechanism is provided at the connection between the upper tube body (100) and the lower tube body (200), a separation tube (300) is provided inside the upper tube body (100), two symmetrical liquid passage holes (301) are provided at the lower end of the separation tube (300), a sealing component is provided at the liquid passage hole (301), and a fixing module is provided at the upper end of the separation tube (300).

2. A centrifuge tube for rapid protein purification according to claim 1, characterized in that the filtration mechanism includes a fixed ring (401), a separation mold (402) is fixedly connected inside the fixed ring (401), a fixing frame (403) is provided at the upper end of the separation mold (402), the fixing frame (403) is fixedly connected to the inner wall of the fixed ring (401), and a fixing block (404) is fixedly connected at the upper end of the fixing frame (403).

3. A centrifuge tube for rapid protein purification according to claim 1, characterized in that the sealing component includes a rotating block (501), the rotating block (501) is rotatably connected to the lower end of the separation tube (300), the outer end of the rotating block (501) is fixedly connected to two symmetrically arranged baffles (502), the position of the baffles (502) corresponds to the liquid passage (301), and the lower end of the rotating block (501) is provided with a groove (503) corresponding to the fixed block (404).

4. A centrifuge tube for rapid protein purification according to claim 1, characterized in that the fixing module includes four limiting blocks (601), the four limiting blocks (601) are fixedly connected to the upper end of the separation tube (300), and the upper end of the upper tube body (100) is provided with limiting grooves (602) corresponding to the limiting blocks (601).

5. A centrifuge tube for rapid protein purification according to claim 1, characterized in that an annular protrusion (700) is provided on the outer wall surface of the separation tube (300), the side of the protrusion (700) away from the separation tube (300) abuts against the inner wall of the upper tube body (100), and the protrusion (700) is made of rubber.

6. A centrifuge tube for rapid protein purification according to claim 1, characterized in that a sealing cap (800) is fixedly connected to the upper end of the separation tube (300).

7. A centrifuge tube for rapid protein purification according to claim 3, characterized in that the upper surface of the baffle (502) completely covers the liquid passage hole (301).

8. A centrifuge tube for rapid protein purification according to claim 4, characterized in that the four limiting blocks (601) are evenly arranged on the separation tube (300).