CN223481142U - Inoculating device suitable for high-pressure state - Google Patents
Inoculating device suitable for high-pressure stateInfo
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- CN223481142U CN223481142U CN202422869573.XU CN202422869573U CN223481142U CN 223481142 U CN223481142 U CN 223481142U CN 202422869573 U CN202422869573 U CN 202422869573U CN 223481142 U CN223481142 U CN 223481142U
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- ball valve
- joint
- stainless steel
- peristaltic pump
- inoculation
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Abstract
本实用新型公开了一种适合高压状态的接种装置，涉及发酵工程技术领域，包括菌种瓶、硅胶软管、蠕动泵、加压装置、不锈钢管和卫生球阀，所述卫生球阀设置在发酵罐的接种口上，所述不锈钢管设置在卫生球阀上，所述加压装置的输出端与不锈钢管通过管道连接，所述加压装置的输入端通过硅胶软管与菌种瓶连接，所述蠕动泵设置在硅胶软管上；本实用新型先将菌种瓶中的菌种液通过蠕动泵输送至加压装置中，再由加压装置将菌种液转移至发酵罐中，避免了硅胶软管、蠕动泵等装置无法在高压状态下进行补料的操作，实现了无需对发酵罐进行降压的操作，就实现了补料操作，提高了工作效率，同时，全程菌种液也不与空气接触，避免了菌种液污染的情况。
The utility model discloses an inoculation device suitable for a high-pressure state, relating to the technical field of fermentation engineering. The device comprises a bacterial seed bottle, a silicone hose, a peristaltic pump, a pressurizing device, a stainless steel pipe and a sanitary ball valve, wherein the sanitary ball valve is arranged on the inoculation port of a fermentation tank, the stainless steel pipe is arranged on the sanitary ball valve, the output end of the pressurizing device is connected to the stainless steel pipe through a pipeline, the input end of the pressurizing device is connected to the bacterial seed bottle through the silicone hose, and the peristaltic pump is arranged on the silicone hose. The utility model first transports the bacterial seed liquid in the bacterial seed bottle to the pressurizing device through the peristaltic pump, and then the pressurizing device transfers the bacterial seed liquid to the fermentation tank, thereby avoiding the inability of the silicone hose, the peristaltic pump and other devices to perform feeding operations under high-pressure conditions, realizing the feeding operation without reducing the pressure of the fermentation tank, thereby improving work efficiency, and at the same time, the bacterial seed liquid does not contact with the air during the whole process, thereby avoiding the contamination of the bacterial seed liquid.
Description
Technical Field
The utility model relates to the technical field of fermentation engineering, in particular to an inoculation device suitable for a high-pressure state.
Background
In fermentation engineering, inoculation is a key step, which directly affects the effect of fermentation and the quality of the product. There are many common inoculation modes, and flame inoculation is a more traditional inoculation method still applied in specific situations. The flame inoculation mode mainly uses the high temperature generated by flame to create a local sterile environment, so as to perform inoculation operation. The flame inoculation mode has a plurality of defects, namely, the risk of bacteria infection is high, the operation requirement is strict, the inoculation opening of an inoculation bottle and a fermentation tank is always under flame protection, the bacteria infection is exposed by improper operation or slow action, the flame is easily influenced by environmental factors such as ventilation and the like and is unstable or even extinguished, the aseptic environment is destroyed, the safety of operators is threatened, the hands are easily burned by the high temperature of the flame during the inoculation operation, the automation is difficult to realize, the manual operation is mainly relied on, the combination with automatic equipment and a system is difficult, the large-scale and high-efficiency fermentation production is not facilitated, and the development requirement of modern fermentation engineering pursuing automation and intelligence cannot be met.
The multipurpose fermentation inoculation device provided by application number 202021712448.3 is compact and small in inoculation port, small in occupied space, convenient to install on various fermentation tanks, good in inoculation port sealing performance, low in bacteria-staining risk, convenient to detach and clean, and wide in overall application, can be used for inoculation under a normal fermentation state, can also be used for supplementing materials under the normal fermentation state, and can be used for filtering treatment on air inside and outside a strain bottle in an inoculation process by the breathing filtration device, so that the inoculation device is safer and more rigorous.
Under certain special fermentation processes or under strict requirements on fermentation conditions, the pressure in the fermentation tank can be increased. For example, in some anaerobic fermentation processes, in order to prevent external oxygen from entering, or in the fermentation process, some gases (such as carbon dioxide, etc.) are generated, a certain positive pressure needs to be maintained, and the pressure may reach 0.3-0.8MPa. In addition, in some processes involving high temperature fermentation or requiring high pressure treatment of the fermented material, the pressure is also controlled within this range to improve the fermentation environment of the microorganism or to improve the fermentation efficiency. The working pressure of the conventional small peristaltic pump is not more than 0.2MPa, the maximum pressure of part of industrial peristaltic pumps can reach about 0.7MPa, and the pressure which can be born by a common silica gel hose is generally about 0.3-0.8MPa, so that the feeding operation in the high-pressure treatment process is difficult to be satisfied by the currently used device. Therefore, in order to solve the above problems, an inoculating device suitable for high pressure condition is provided to meet the practical requirement.
Disclosure of utility model
The utility model provides an inoculation device suitable for a high-pressure state, which solves the technical problem that the conventional inoculation device is difficult to meet the requirement of feeding operation in a high-pressure treatment process.
In order to solve the technical problems, the inoculation device suitable for the high-pressure state comprises a strain bottle, a silica gel hose, a peristaltic pump, a pressurizing device, a stainless steel tube and a sanitary ball valve, wherein the sanitary ball valve is arranged on an inoculation port of a fermentation tank, the stainless steel tube is arranged on the sanitary ball valve, the output end of the pressurizing device is connected with the stainless steel tube through a pipeline, the input end of the pressurizing device is connected with the strain bottle through the silica gel hose, and the peristaltic pump is arranged on the silica gel hose.
In some embodiments, the pressurizing device comprises a pressurizing container, the pressurizing container comprises a container shell, a pressurizing piston is arranged in the container shell, the pressurizing piston divides the interior of the container shell into a liquid storage cavity and a cavity, a first connector and a second connector are respectively arranged on one side of the cavity of the container shell, and a third connector and a fourth connector are respectively arranged on one side of the liquid storage cavity of the container shell.
In some embodiments, the pressurized container further comprises a first ball valve, a connecting tube, an air compressor, and a second ball valve, wherein an output end of the first ball valve is connected with the fourth joint, an input end of the connecting tube is connected with the third joint, an output end of the air compressor is connected with the second joint, and an air inlet end of the second ball valve is connected with the first joint.
In some embodiments, the input end of the first ball valve is connected with the output end of the silica gel hose after being provided with the one-way valve, and the output end of the connecting pipe is connected with the input end of the stainless steel pipe.
In some embodiments, a sealing plug is arranged at the bottle mouth of the strain bottle, a breathing tube is arranged on the sealing plug, and a breathing filter is arranged on the breathing tube.
In some embodiments, the silicone hose is sleeved on a pump head roller of the peristaltic pump.
Compared with the related art, the inoculation device suitable for the high-pressure state has the following beneficial effects:
The utility model provides the inoculation device suitable for the high-pressure state, firstly, the strain liquid in the strain bottle is conveyed to the pressurizing device through the peristaltic pump, and then the strain liquid is transferred to the fermentation tank through the pressurizing device, so that the situation that the devices such as a silica gel hose, the peristaltic pump and the like cannot perform material supplementing operation under the high-pressure state is avoided, the material supplementing operation is realized without the operation of reducing the pressure of the fermentation tank, the working efficiency is improved, and meanwhile, the strain liquid is not contacted with the air in the whole process, and the strain liquid pollution is avoided.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present utility model;
FIG. 2 is a schematic view of a pressurizing device according to the present utility model;
FIG. 3 is a schematic cross-sectional view of the pressurized container of the present utility model.
The reference numbers in the figure are 1, a strain bottle, 2, a respiratory tube, 3, a respiratory filter, 4, a silica gel hose, 5, a peristaltic pump, 6, a pressurizing device, 7, a stainless steel tube, 8, a sanitary ball valve, 9, a fermentation tank, 10, an inoculation port, 61, a pressurizing container, 62, a first ball valve, 63, a one-way valve, 64, a connecting pipe, 65, an air compressor, 66, a second ball valve, 611, a container shell, 612, a pressurizing piston, 613, a first connector, 614, a second connector, 615, a third connector, 616 and a fourth connector.
Detailed Description
Example 1
The embodiment provides an inoculation device suitable for a high-pressure state, as shown in fig. 1-3, the inoculation device comprises a strain bottle 1, a silica gel hose 4, a peristaltic pump 5, a pressurizing device 6, a stainless steel pipe 7 and a sanitary ball valve 8, wherein the sanitary ball valve 8 is arranged on an inoculation port 10 of a fermentation tank 9, the stainless steel pipe 7 is arranged on the sanitary ball valve 8, the output end of the pressurizing device 6 is connected with the stainless steel pipe 7 through a pipeline, the input end of the pressurizing device 6 is connected with the strain bottle 1 through the silica gel hose 4, and the peristaltic pump 5 is arranged on the silica gel hose 4.
In this embodiment, the fermentation tank 9 is a sealed cavity with pressure, the inoculation port 10 and the fermentation tank 9 are integrally formed, the sanitary ball valve 8 is connected with the inoculation port 10 and the stainless steel pipe 7 through the sanitary clamp, the stainless steel pipe 7 is made of food-grade stainless steel materials, the peristaltic pump 5 conveys the strain in the strain bottle 1 to the pressurizing device 6 through the silica gel hose 4, the pipeline between the silica gel hose 4 and the pressurizing device 6 is closed, and then the strain enters the fermentation tank 9 from the pressurizing device 6 through the stainless steel pipe 7, the sanitary ball valve 8 and the inoculation port 10, so that the pressurized feeding operation of the fermentation tank 9 is realized. The repeated depressurization and pressurization of the fermentation tank 9 can also be avoided, and the feeding efficiency is improved.
Example two
As shown in fig. 3, the pressurizing device 6 of the present embodiment includes, on the basis of the first embodiment, a pressurizing container 61, the pressurizing container 61 including a container housing 611, a pressurizing piston 612 being provided inside the container housing 611, the pressurizing piston 612 dividing the inside of the container housing 611 into a liquid storage chamber and a cavity, a first joint 613 and a second joint 614 being provided on the cavity side of the container housing 611, and a third joint 615 and a fourth joint 616 being provided on the liquid storage chamber side of the container housing 611, respectively.
In this embodiment, the volume of the liquid storage chamber and the cavity in the vessel housing 611 is changed by providing a pressurizing piston 612 that can move up and down in the vessel housing 611.
Example III
As shown in fig. 2, the pressurized container 61 of the present embodiment further includes, on the basis of the second embodiment, a first ball valve 62, a connection pipe 64, an air compressor 65, and a second ball valve 66, wherein an output end of the first ball valve 62 is connected to a fourth joint 616, an input end of the connection pipe 64 is connected to a third joint 615, an output end of the air compressor 65 is connected to a second joint 614, and an intake end of the second ball valve 66 is connected to the first joint 613. The input end of the first ball valve 62 is provided with a one-way valve 63 and then is connected with the output end of the silica gel hose 4, and the output end of the connecting pipe 64 is connected with the input end of the stainless steel pipe 7.
In this embodiment, the first ball valve 62 may control the opening and closing of the pipeline between the silicone hose 4 and the pressurizing device 6, and after the first ball valve 62 and the second ball valve 66 are opened and the sanitary ball valve 8 is closed, the peristaltic pump 5 may be used to convey the strain liquid in the strain bottle 1 to the liquid storage cavity of the pressurizing container 61, the volume of the liquid storage cavity is increased, and the pressurizing piston 612 moves upward at this time. In which it is possible to avoid damage caused by excessive pressure in the silicone hose 4 due to the fact that the first ball valve 62 is not completely closed by the provision of the check valve 63. The first ball valve 62 and the second ball valve 66 are closed, at this time, the air compressor 65 is opened, the pressure of the air compressor 65 is set to be the same as the pressure in the fermentation tank 9, the sanitary ball valve 8 is opened, the pressure of the air compressor 65 is continuously increased, the pressurizing piston 612 is made to move downwards, the strain liquid sequentially flows into the fermentation tank 9 through the connecting pipe 64, the stainless steel pipe 7 and the sanitary ball valve 8, and then the sanitary ball valve 8 is closed.
Example IV
On the basis of the first embodiment, the bottle mouth of the strain bottle 1 of the embodiment is provided with a sealing plug, the sealing plug is provided with a breathing tube 2, and the breathing tube 2 is provided with a breathing filter 3.
In the embodiment, the strain bottle 1 is made of glass or metal and is used for containing strain liquid or other nutrient solution and acid-base solution, the breathing filter 3 is preferably made of polytetrafluoroethylene or polyvinylidene fluoride,
Example five
On the basis of the first embodiment, the middle part of the silica gel hose 4 of the embodiment is sleeved on a pump head roller of the peristaltic pump 5. The inner diameter of the silicone hose 4 is slightly smaller than the outer diameter of the pump head roller of the peristaltic pump 5, so that the silicone hose 4 can be tightly sleeved on the roller during installation.
In the embodiment, the brand and model selected by the silica gel hose 4 are the Baoding Lei Fu BT101F, so that accurate feeding is realized.
The working principle is that the strain liquid in the strain bottle 1 is firstly conveyed to the pressurizing device 6 through the peristaltic pump 5, and then the strain liquid is transferred to the fermentation tank 9 through the pressurizing device 6, so that the situation that the devices such as the silica gel hose 4, the peristaltic pump 5 and the like cannot perform material supplementing operation under a high pressure state is avoided, the material supplementing operation is realized without the operation of reducing the pressure of the fermentation tank 9, the working efficiency is improved, and meanwhile, the strain liquid is not contacted with air in the whole process, and the strain liquid pollution is avoided.
The experimental steps are as follows:
s1, firstly, connecting devices according to the figure 1;
s2, keeping the sanitary ball valve 8 in a closed state, the second ball valve 66 and the first ball valve 62 in an open state, and ensuring that the position of the pressurizing piston 612 is not excessively high;
S3, starting a peristaltic pump 5, wherein the strain liquid in the strain bottle 1 flows into a liquid storage cavity of the pressurized container 61 through a one-way valve 63, a first ball valve 62 and a fourth connector 616 by a silica gel hose 4, and the pressurized piston 612 moves upwards along with the volume increase of the strain liquid in the liquid storage cavity, so that air in the cavity is discharged from the first connector 613 through a second ball valve 66;
S4, after a certain amount of strain liquid is contained in the container shell 611, the peristaltic pump 5 is closed, and then the second ball valve 66 and the first ball valve 62 are closed;
S5, turning on the air compressor 65, and setting the pressure of the air compressor 65 to be the same as the pressure in the fermentation tank 9;
and S6, after the pressure at the output end of the air compressor 65 reaches the level of the fermentation tank 9, opening the sanitary ball valve 8, continuously increasing the pressure of the air compressor 65, so that the pressurizing piston 612 moves downwards, the strain liquid is completely discharged, the strain liquid flows into the fermentation tank 9 through the connecting pipe 64, the stainless steel pipe 7 and the sanitary ball valve 8 in sequence, and after the strain liquid is completely discharged, the sanitary ball valve 8 is closed, so that the feeding operation is completed.
Claims (6)
1. The utility model provides an inoculation device suitable for high pressure state, includes bacterial bottle (1), silica gel hose (4), peristaltic pump (5), supercharging device (6), stainless steel pipe (7) and sanitary ball valve (8), wherein sanitary ball valve (8) set up on inoculation mouth (10) of fermentation cylinder (9), stainless steel pipe (7) set up on sanitary ball valve (8), the output and the stainless steel pipe (7) of supercharging device (6) pass through the pipe connection, the input of supercharging device (6) is connected with bacterial bottle (1) through silica gel hose (4), peristaltic pump (5) set up on silica gel hose (4).
2. Inoculation device suitable for high pressure conditions according to claim 1, characterized in that the pressurizing device (6) comprises a pressurizing container (61), the pressurizing container (61) comprises a container housing (611), the interior of the container housing (611) is provided with a pressurizing piston (612), the pressurizing piston (612) divides the interior of the container housing (611) into a liquid storage cavity and a cavity, one side of the cavity of the container housing (611) is provided with a first joint (613) and a second joint (614), respectively, and one side of the liquid storage cavity of the container housing (611) is provided with a third joint (615) and a fourth joint (616), respectively.
3. The inoculating device suitable for high pressure conditions according to claim 2, wherein the pressurized container (61) further comprises a first ball valve (62), a connecting pipe (64), an air compressor (65) and a second ball valve (66), wherein the output end of the first ball valve (62) is connected with a fourth joint (616), the input end of the connecting pipe (64) is connected with a third joint (615), the output end of the air compressor (65) is connected with a second joint (614), and the air inlet end of the second ball valve (66) is connected with the first joint (613).
4. A device according to claim 3, characterized in that the input end of the first ball valve (62) is provided with a one-way valve (63) and then connected with the output end of the silicone hose (4), and the output end of the connecting pipe (64) is connected with the input end of the stainless steel pipe (7).
5. The inoculation device suitable for the high-pressure state according to claim 1, wherein a sealing plug is arranged at the bottle mouth of the strain bottle (1), a breathing tube (2) is arranged on the sealing plug, and a breathing filter (3) is arranged on the breathing tube (2).
6. An inoculating device suitable for high pressure conditions according to claim 1, characterized in that the silicone hose (4) is sleeved on the pump head roller of peristaltic pump (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202422869573.XU CN223481142U (en) | 2024-11-25 | 2024-11-25 | Inoculating device suitable for high-pressure state |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202422869573.XU CN223481142U (en) | 2024-11-25 | 2024-11-25 | Inoculating device suitable for high-pressure state |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN223481142U true CN223481142U (en) | 2025-10-28 |
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ID=97438989
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202422869573.XU Active CN223481142U (en) | 2024-11-25 | 2024-11-25 | Inoculating device suitable for high-pressure state |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN223481142U (en) |
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2024
- 2024-11-25 CN CN202422869573.XU patent/CN223481142U/en active Active
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