CN223458321U

CN223458321U - A biological enzyme purification device

Info

Publication number: CN223458321U
Application number: CN202422733214.1U
Authority: CN
Inventors: 张革; 谢宇; 唐章勇
Original assignee: Debord Chengdu Biotechnology Co ltd
Current assignee: Debord Chengdu Biotechnology Co ltd
Priority date: 2024-11-11
Filing date: 2024-11-11
Publication date: 2025-10-21
Anticipated expiration: 2034-11-11

Abstract

The utility model provides a bio-enzyme purification device, which belongs to the technical field of bio-enzyme preparation equipment. It includes a mounting frame, a first cylinder is provided on the mounting frame, a coarse filter mechanism is provided in the first cylinder, a first liquid inlet pipe is provided at the top of the first cylinder, an air inlet pipe is connected to the outer wall, a mounting box is provided on the mounting frame, the first cylinder is connected to the mounting box, a sewage pipe is connected to the bottom of the mounting box, an exhaust pipe is connected to the top, a fine filter mechanism and a second liquid outlet pipe are connected to one side wall of the mounting box, and a valve body is provided in the exhaust pipe. During the process of culture fluid being fed from the first cylinder into the mounting box, the culture fluid gradually fills the mounting box and discharges gas from the exhaust pipe. After the valve body closes the exhaust pipe, the culture fluid is filtered through the filter layer and discharged, thereby obtaining a purified bio-enzyme solution. When the culture fluid drops below the top of the filter layer, the valve is reopened to exhaust the air in time, thereby avoiding the generation of bubbles in the bio-enzyme solution. This device has the advantage of improving the purification effect of the bio-enzyme solution.

Description

Biological enzyme purification device

Technical Field

The utility model relates to the technical field of biological enzyme preparation equipment, in particular to a biological enzyme purification device.

Background

During the production of protease, the protease needs to be purified and separated, namely, the biological tissue is firstly crushed in the extraction process, then clear liquid is taken and added with medicament, and then the liquid is poured into enzyme extraction equipment, and impurities are filtered out through the equipment to obtain the liquid with high protease content.

The patent with the publication number of CN212925016U discloses a protease purifying device, which comprises a supporting frame, a shell fixed on the supporting frame and a first cylinder fixed on the shell, wherein a centrifugal filtering mechanism for centrifugally filtering a solution containing protease is arranged in the first cylinder, a centrifugal power mechanism connected with the centrifugal filtering mechanism is arranged in the shell, a fine filtering mechanism is also arranged in the shell, a liquid power mechanism is also arranged in the shell, the liquid power mechanism sends liquid centrifugally filtered in the first cylinder into the fine filtering mechanism, the protease in the solution is further purified and separated through the fine filtering mechanism, the liquid power mechanism is a pump body, the liquid inlet end of the liquid power mechanism is connected with the first cylinder, and the liquid outlet end of the liquid power mechanism is connected with the fine filtering mechanism.

However, when in actual use, the experimenter cannot accurately observe the content of the preliminarily filtered solution in the first barrel, the solution in the first barrel is completely pumped into the fine filtering mechanism along with the pump body, the pump body can idle, abrasion of the pump body and bubbling of the finely filtered biological enzyme solution are easily caused, the service life of the pump body is reduced, and the follow-up experiment is influenced.

Disclosure of utility model

In view of the above, the present utility model provides a biological enzyme purification apparatus.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

The utility model provides a biological enzyme purification device, including the installation frame, be provided with first barrel in the installation frame, be provided with the straining device in the first barrel, the top of first barrel is provided with just straining device's first feed liquor pipe, be connected with first valve on the first feed liquor pipe, be provided with the mounting box in the below of being located first barrel in the installation frame, the bottom of first barrel is connected with first drain pipe, the other end and the mounting box of first drain pipe are connected with the blow off pipe, the top of mounting box is connected with the blast pipe, be connected with the intake pipe on the outer wall of first barrel, the external air supply of intake pipe is connected with the second drain pipe on one side lateral wall of mounting box, be provided with the fine filtration mechanism between mounting box and the second drain pipe, be provided with the valve body that is used for the evacuation mounting box internal air and restriction solution to get into the blast pipe in the blast pipe.

Further, the rough filtering mechanism comprises a second cylinder body which is rotatably arranged in the first cylinder body, the top opening and the side wall of the second cylinder body are hollowed, a first driving motor is arranged on the mounting rack, an output shaft of the first driving motor stretches into the first cylinder body and is coaxially and fixedly connected to the bottom wall of the second cylinder body, and a filtering sleeve is coaxially and fixedly arranged on the inner wall of the second cylinder body.

Further, the fine filtering mechanism comprises a mounting frame and a filtering layer, wherein the mounting frame is arranged on the side wall of the mounting box, a mounting opening communicated with the mounting box and the second liquid outlet pipe is formed in the mounting frame in a penetrating mode, and the filtering layer is arranged in the mounting opening.

Further, the bottom of the blow-down pipe is detachably connected with a sewage collection tank.

Further, the valve body comprises a floating ball and a connecting frame, an exhaust port is formed in the mounting box, the exhaust pipe is communicated with the exhaust port, the connecting frame is arranged in the mounting box and located right below the exhaust port, the floating ball is vertically and movably arranged in the connecting frame, and the floating ball can block the exhaust port upwards.

Further, be connected with the blowout prevention subassembly on the blast pipe, the blowout prevention subassembly is including covering in proper order to establish the first blowout prevention lid and the second blowout prevention lid in the export outside of blast pipe, and a plurality of first export and second export have evenly been seted up respectively along self circumference on the lateral wall of first blowout prevention lid and the second blowout prevention lid, and first export and second export dislocation each other.

Further, the inner top wall of the mounting box is provided with an inverted conical guide groove, and the exhaust port is connected to the topmost end of the guide groove.

When the biological enzyme is purified, the culture solution is injected into the second cylinder body in the first cylinder body through the first liquid inlet pipe, the first liquid inlet pipe is closed, the second cylinder body is rotated, the culture solution in the second cylinder body is subjected to primary filtration from the filter sleeve on the side wall of the second cylinder body, after the primary filtration, the air source is used for charging air into the first cylinder body from the air inlet pipe, the culture solution in the first cylinder body is sent into the mounting box from the first liquid outlet pipe, when the mounting box is empty with air, the valve body is closed after the mounting box is full of the culture solution, the culture solution is forced to be filtered by the filter layer and then discharged, the purified biological enzyme solution is obtained, the valve is opened to prevent air from being discharged from the second liquid outlet pipe to generate bubbles in the biological enzyme solution when the culture solution in the mounting box is reduced below the top of the filter layer, and the biological enzyme solution purification effect is improved.

Drawings

FIG. 1 is a schematic diagram showing the overall structure of a biological enzyme purification apparatus according to an embodiment of the present application.

Fig. 2 is an enlarged partial schematic view of the portion a in fig. 1.

The device comprises a mounting rack, 11, a first barrel, 111, a first liquid outlet pipe, 112, an air inlet pipe, 12, a first liquid inlet pipe, 121, a first valve, 21, a second barrel, 22, a first driving motor, 23, a filter sleeve, 3, a mounting box, 31, an exhaust pipe, 32, a second liquid outlet pipe, 33, a guide groove, 5, a drain pipe, 51, a dirt collecting tank, 61, a mounting frame, 62, a filter layer, 71, a floating ball, 72, a connecting frame, 73, an exhaust port, 81, a first blowout prevention cover, 82 and a second blowout prevention cover.

Detailed Description

In order to better understand the above technical solutions, the following detailed description will refer to the accompanying drawings and specific embodiments.

The embodiment of the application discloses a biological enzyme purification device, referring to fig. 1 and 2, which comprises a mounting frame 1, wherein a first cylinder 11 is arranged on the mounting frame 1, the interior of the first cylinder 11 is hollow and is provided with a rough filtration mechanism, the top of the first cylinder 11 is connected with a first liquid inlet pipe 12, the first liquid inlet pipe 12 is connected with a valve, one end of the first liquid inlet pipe 12 extending into the first cylinder 11 is opposite to the rough filtration mechanism, and biological enzyme culture solution enters the first cylinder 11 from the first liquid inlet pipe 12 and is released into the rough filtration mechanism. The mounting frame 1 is provided with the mounting box 3 below the first barrel 11, and the bottom of first barrel 11 is connected with first drain pipe 111, and the other end and the mounting box 3 of first drain pipe 111 are connected. The outer wall of the first barrel 11 is connected with an air inlet pipe 112, the air inlet pipe 112 is externally connected with an air source, the air source can be clean air, the bottom of the installation box 3 is connected with a sewage drain pipe 5, the top of the installation box 3 is connected with an exhaust pipe 31, one side wall of the installation box 3 is connected with a second liquid outlet pipe 32, and a fine filtering mechanism is arranged between the installation box 3 and the second liquid outlet pipe 32. An exhaust pipe 31 is connected to the top wall of the mounting box 3, and a valve body for exhausting air in the mounting box 3 and limiting solution to enter the exhaust pipe 31 is arranged in the exhaust pipe 31.

When the device is in operation, after the biological enzyme culture solution is subjected to rough filtration by the rough filtration mechanism, the culture solution enters the first cylinder 11, and air is injected into the first cylinder 11 through an air source, so that the culture solution in the first cylinder 11 enters from the first liquid outlet pipe 111 at the bottom of the first cylinder 11 and flows into the mounting box 3. In the mounting box 3, original air is discharged from the exhaust pipe 31 through the valve body until the mounting box 3 is filled with the culture solution, the valve body closes the exhaust pipe 31 and continuously injects air into the first barrel 11, the culture solution can be extruded to pass through the fine filtering mechanism, the filtered and purified biological enzyme solution is obtained, and the filtered impurities are precipitated in the mounting box 3 and enter the sewage pipe 5 for storage.

In the embodiment of the application, the rough filtering mechanism comprises a second cylinder body 21 rotatably arranged in a first cylinder body 11, the top opening and the side wall of the second cylinder body 21 are hollowed out, and a filtering sleeve 23 is coaxially and fixedly arranged on the inner wall of the second cylinder body 21. The first driving motor 22 is fixedly arranged in the mounting frame 1, an output shaft of the first driving motor 22 stretches into the first cylinder 11 and is coaxially and fixedly connected to the bottom wall of the second cylinder 21, one end of the first liquid inlet pipe 12 stretching into the first cylinder 11 is positioned right above an upper opening of the second cylinder 21, the first driving motor 22 works to drive the second cylinder 21 to rotate, and therefore culture solution in the second cylinder 21 is attached to the inner wall of a filter cylinder under the action of centrifugation, filtered by the filter cylinder and discharged into the first cylinder 11.

The fine filtering mechanism comprises a mounting frame 61 and a filtering layer 62, wherein the mounting frame 61 is arranged on the side wall of the mounting box 3, a mounting opening which is communicated with the mounting box 3 and the second liquid outlet pipe 32 is formed in the mounting frame 61 in a penetrating manner, the filtering layer 62 is arranged in the mounting opening, and the aperture of the filtering layer 62 is smaller than that of the filtering sleeve 23.

In the embodiment of the application, the end of the drain pipe 5 away from the mounting box 3 is detachably connected with the dirt collecting tank 51, the dirt collecting tank 51 is used for collecting the impurities filtered in the mounting box 3, and the dirt collecting tank 51 and the drain pipe 5 can be connected in a threaded connection mode. In other embodiments, a second valve may be installed on the drain pipe 5, and the opening and closing of the drain pipe 5 may be controlled by the valve.

In the embodiment of the application, the valve body comprises a floating ball 71 and a connecting frame 72, the mounting box 3 is internally provided with an exhaust port 73, the exhaust port 73 is not lower than the upper edge of the filter layer 62 in the fine filtration mechanism, the exhaust pipe 31 is communicated with the exhaust port 73, the connecting frame 72 is a hollowed frame body, the connecting frame 72 is fixedly arranged on the inner top wall of the mounting box 3 and is positioned right below the exhaust port 73, the floating ball 71 is vertically and movably arranged in the connecting frame 72, the floating ball 71 can adopt a hollow plastic ball to arrange a rubber outer layer on the surface of the plastic ball, the diameter of the floating ball 71 is larger than that of the exhaust port 73, and the floating ball 71 can upwards seal the exhaust port 73.

Further, a blowout prevention assembly is connected to the exhaust pipe 31, the blowout prevention assembly comprises a first blowout prevention cover 81 and a second blowout prevention cover 82 which are sequentially covered on the outer side of an outlet of the exhaust pipe 31, a plurality of first outlets and second outlets are uniformly formed in the side walls of the first blowout prevention cover 81 and the second blowout prevention cover 82 along the circumferential direction of the side walls, and the first outlets and the second outlets are staggered. When the culture solution in the exhaust pipe 31 upwards gushes out, the first blowout prevention cover 81 is used for blocking the culture solution upwards gushed out, blocking and slowing down the upward blowout impact force of the culture solution, so that the culture solution flows out from the first outlets around the first blowout prevention cover 81, and the second blowout prevention cover 82 is used for secondarily blocking the culture solution, so that the impact force of the culture solution is further slowed down.

Further, the inner top wall of the mounting box 3 is provided with an inverted tapered guide groove 33, and the exhaust port 73 is connected to the uppermost end of the guide groove 33. The air in the mounting box 3 can be collected more easily through the inverted conical guide groove 33, so that the air in the mounting box 3 can be emptied, and air still can be discharged from the fine filtration mechanism when the culture solution passes through the fine filtration mechanism, so that bubbles in the purified biological enzyme solution caused by fine filtration of the culture solution are avoided.

It will be apparent to those skilled in the art that while preferred embodiments of the present utility model have been described, additional variations and modifications may be made to these embodiments once the basic inventive concepts are known to those skilled in the art. It is therefore intended that the following claims be interpreted as including the preferred embodiments and all such alterations and modifications as fall within the scope of the utility model. It will be apparent to those skilled in the art that various modifications and variations can be made to the present utility model without departing from the spirit or scope of the utility model. Thus, it is intended that the present utility model also include such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.

Claims

1. The biological enzyme purification device is characterized by comprising an installation frame (1), a first barrel (11) is arranged on the installation frame (1), a straining mechanism is arranged in the first barrel (11), a first liquid inlet pipe (12) opposite to the straining mechanism is arranged at the top of the first barrel (11), a first valve (121) is connected to the first liquid inlet pipe (12), an installation box (3) is arranged below the first barrel (11) on the installation frame (1), a first liquid outlet pipe (111) is connected to the bottom of the first barrel (11), the other end of the first liquid outlet pipe (111) is connected with the installation box (3), a drain pipe (5) is connected to the bottom of the installation box (3), an exhaust pipe (31) is connected to the top of the installation box (3), an air inlet pipe (112) is connected to the outer wall of the first barrel (11), a second liquid outlet pipe (32) is connected to the side wall of the installation box (3), and a second liquid outlet pipe (32) is arranged on one side wall of the installation box (3), and the second liquid outlet pipe (3) is connected to the drain pipe (5), and the air outlet pipe (31) is arranged between the installation box and the second fine filter body (31) and the air outlet pipe (31) is used for exhausting.

2. The biological enzyme purification device according to claim 1, wherein the straining mechanism comprises a second cylinder (21) rotatably arranged in the first cylinder (11), an opening at the top of the second cylinder (21) and a hollow-out arrangement at the side wall are arranged, a first driving motor (22) is arranged on the mounting rack (1), an output shaft of the first driving motor (22) extends into the first cylinder (11) and is coaxially and fixedly connected to the bottom wall of the second cylinder (21), and a filter sleeve (23) is coaxially and fixedly arranged on the inner wall of the second cylinder (21).

3. The biological enzyme purification device according to claim 2, wherein the fine filtration mechanism comprises a mounting frame (61) and a filter layer (62), the mounting frame (61) is arranged on the side wall of the mounting box (3), the mounting frame (61) is provided with a mounting opening which is communicated with the mounting box (3) and the second liquid outlet pipe (32) in a penetrating manner, and the filter layer (62) is arranged in the mounting opening.

4. A biological enzyme purification device according to claim 1, characterized in that the bottom of the drain pipe (5) is detachably connected with a dirt collection tank (51).

5. The biological enzyme purification device according to any one of claims 1-4, wherein the valve body comprises a floating ball (71) and a connecting frame (72), an exhaust port (73) is formed in the mounting box (3), the exhaust pipe (31) is communicated with the exhaust port (73), the connecting frame (72) is arranged in the mounting box (3) and located right below the exhaust port (73), the floating ball (71) is vertically movably arranged in the connecting frame (72), and the floating ball (71) can block the exhaust port (73) upwards.

6. The biological enzyme purification device according to claim 5, wherein the exhaust pipe (31) is connected with a blowout prevention assembly, the blowout prevention assembly comprises a first blowout prevention cover (81) and a second blowout prevention cover (82) which are sequentially covered outside an outlet of the exhaust pipe (31), a plurality of first outlets and second outlets are uniformly formed in the side walls of the first blowout prevention cover (81) and the second blowout prevention cover (82) along the circumferential direction of the side walls of the first blowout prevention cover and the second blowout prevention cover, and the first outlets and the second outlets are staggered with each other.

7. The biological enzyme purification apparatus according to claim 5, wherein the inner top wall of the mounting box (3) is provided with an inverted cone-shaped guide groove (33), and the air vent (73) is connected to the topmost end of the guide groove (33).