CN223430432U - A test kit for convenient sample bottle placement - Google Patents

Abstract

The utility model discloses a kit convenient for placing sample bottles, which belongs to the technical field of kits and comprises a kit used for storing the sample bottles, wherein centrifugal separation equipment used for carrying out centrifugal operation on the sample bottles is arranged in the kit, the centrifugal separation equipment comprises a driving mechanism, an auxiliary mechanism and a rotating mechanism, the driving mechanism is arranged in the kit, the rotating mechanism is provided with a plurality of rotating mechanisms which are equidistantly and circumferentially arranged on the driving mechanism, and the auxiliary mechanism is arranged in the kit.

Description

Kit convenient to sample bottle is placed

Technical Field

The utility model relates to the technical field of kits, in particular to a kit convenient for placing a sample bottle.

Background

In recent years, exosome technology has become a hotspot for cancer detection and prognosis due to its non-invasive and abundant biomarker content. The method aims at realizing more accurate laryngeal cancer prognosis evaluation by combining saliva exosome extraction technology and embedding the genes into a model as potential markers, wherein exosome extraction and marker detection adopt advanced acoustic fluid technology (such as Acoustofluidic platform) to extract exosomes in saliva of patients, the technology can efficiently separate and extract exosomes, ensure sample purity and stability of biomarkers, extract RNA/DNA from exosomes, detect expression conditions of TIMP1, MNX1, LCK and MMP3, and analyze by using a droplet DIGITAL PCR and other high-precision molecular detection method.

Because the exosome after the sample is placed and is stored in required sample bottle inside, in order to embody the characteristics of "timeliness", "accuracy" and portable that the exosome of sample draws, consequently can place required kit in placing all sample bottles that accomplish and store, play portable, can take the effect of detecting at any time, and in order to the accuracy that the exosome sample goes on in sample bottle inside, at present, no kit possesses the effect of storing and carrying out the centrifugation operation to required sample bottle, and in order to sample detection more accurate analysis, detect and research, carry out the centrifugation to the sample and carry out impurity separation and play vital effect, thereby this device provides a kit convenient to sample bottle is placed.

Disclosure of utility model

The utility model aims at overcoming the defects of the prior art and providing a kit convenient for placing a sample bottle so as to solve the technical problems.

The embodiment of the utility model adopts the following technical scheme that the kit comprises a kit for storing sample bottles, wherein centrifugal separation equipment for carrying out centrifugal operation on the sample bottles is arranged in the kit, the centrifugal separation equipment comprises a driving mechanism, an auxiliary mechanism and a rotating mechanism, the driving mechanism is arranged in the kit, the rotating mechanism is provided with a plurality of rotating mechanisms, the rotating mechanisms are all equidistantly and circumferentially arranged on the driving mechanism, the auxiliary mechanism is arranged in the kit, the rotating mechanisms and the auxiliary mechanism are respectively in a matched rotating state, the kit comprises a reagent chamber and a reagent cover, and the reagent cover is arranged on the reagent chamber and can be opened.

Further, the driving mechanism comprises a driving motor, a driving wheel, a driven wheel and a belt, wherein the driving motor is arranged inside the kit, the driving wheel is arranged at the output end of the driving motor, the driven wheel is rotationally connected inside the kit and is positioned at the side of the driving wheel, and the belt is sleeved on the driving wheel and the driven wheel.

Further, a control system for electrically connecting the driving motor is arranged on the kit, the driving motor is a direct-current speed regulating motor, a control display screen is arranged on the control system, and the control display screen can operate and control the driving motor to run for touching the display screen.

Further, the auxiliary mechanism comprises an auxiliary shaft and an auxiliary bevel gear, the middle of the driven wheel is hollow, the auxiliary shaft is arranged on the kit, the upper end of the auxiliary shaft penetrates through the hollow position of the driven wheel, and the auxiliary bevel gear is arranged at the upper end of the auxiliary shaft.

Further, a plurality of rotary mechanism all includes runing rest, rotation axis and rotatory storage cylinder, the runing rest sets up in from the driving wheel upper end, the upper end of runing rest sets up for the slope, the rotation axis sets up in the upper end of runing rest and towards the inboard, the rotation axis is connected for rotating with the runing rest, the cover is equipped with the rotatory gear with supplementary helical gear looks adaptation looks meshing on the rotation axis, rotatory storage cylinder sets up the upper end at the rotation axis.

Further, the rotary storage cylinder is internally provided with a storage groove which is arranged in a matched size with a traditional sample bottle.

Further, the inclination angles of the upper ends of the plurality of rotating brackets are forty degrees.

The above at least one technical scheme adopted by the embodiment of the utility model can achieve the following beneficial effects:

The utility model can be used for placing and storing a plurality of sample bottles, is convenient for carrying and can take the sample bottles at any time, and in the storing process, the sample bottles which can be stored by the kit can be centrifugally rotated by starting the centrifugal separation equipment, and the exosomes stored in the sample bottles can be separated from the impurities by centrifugation, so that the components of exosome samples are more uniform, and the follow-up samples are more accurately analyzed, detected and researched.

Drawings

The accompanying drawings, which are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate embodiments of the utility model and together with the description serve to explain the utility model and do not constitute a limitation on the utility model. In the drawings:

FIG. 1 is a schematic view showing the structure of the reagent cap in the opened state of the present utility model;

FIG. 2 is a schematic perspective view of the present utility model;

FIG. 3 is a schematic perspective view of a centrifugal separator according to the present utility model;

FIG. 4 is a schematic perspective view of a driving mechanism and a rotating mechanism according to the present utility model;

Fig. 5 is an enlarged view at a in fig. 4.

Reference numerals

The reagent cassette 1, the reagent chamber 11, the reagent cover 12, the centrifugal separation device 2, the driving mechanism 21, the driving motor 211, the driving pulley 212, the driven pulley 213, the belt 214, the control display 215, the auxiliary mechanism 22, the auxiliary shaft 221, the auxiliary bevel gear 222, the rotating mechanism 23, the rotating bracket 231, the rotating shaft 232, the rotating storage cylinder 233, the rotating gear 234, and the storage tank 235.

Detailed Description

In order to make the objects, technical solutions and advantages of the present utility model more apparent, the technical solutions of the present utility model will be clearly and completely described below with reference to specific embodiments of the present utility model and corresponding drawings. It will be apparent that the described embodiments are only some, but not all, embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

The following describes in detail the technical solutions provided by the embodiments of the present utility model with reference to the accompanying drawings.

The embodiment of the utility model provides a kit convenient for placing a sample bottle, which comprises a kit 1 used for storing the sample bottle, wherein a centrifugal separation device 2 used for carrying out centrifugal operation on the sample bottle is arranged inside the kit 1, the centrifugal separation device 2 comprises a driving mechanism 21, an auxiliary mechanism 22 and a rotating mechanism 23, the driving mechanism 21 is arranged inside the kit 1, the rotating mechanism 23 is provided with a plurality of rotating mechanisms 23 which are all equidistantly and circumferentially arranged on the driving mechanism 21, the auxiliary mechanism 22 is arranged inside the kit 1, the rotating mechanisms 23 and the auxiliary mechanism 22 are respectively in a matched rotating state, the kit 1 comprises a reagent chamber 11 and a reagent cover 12, and the reagent cover 12 is arranged on the reagent chamber 11 and can be opened;

the device can be used for placing and storing a plurality of sample bottles, and in the storage process, the sample bottles which can be stored in the kit 1 can be subjected to centrifugal rotation operation through the starting of the centrifugal separation equipment 2, and the exosomes stored in the sample bottles can be separated from the impurities through centrifugation, so that the components of exosome samples are more uniform, and further, the follow-up samples are more accurately analyzed, detected and researched.

In a further preferred embodiment of the present utility model, the driving mechanism 21 includes a driving motor 211, a driving wheel 212, a driven wheel 213 and a belt 214, the driving motor 211 is disposed inside the kit 1, the driving wheel 212 is disposed at an output end of the driving motor 211, the driven wheel 213 is rotatably connected inside the kit 1 and is located at a side of the driving wheel 212, the belt 214 is sleeved on the driving wheel 212 and the driven wheel 213, a control system for electrically connecting the driving motor 211 is disposed on the kit 1, the driving motor 211 is a dc speed regulating motor, a control display screen 215 is disposed on the control system, and the control display screen 215 is a touch display screen operable to control the driving motor 211 to operate;

The driving motor 211 is a direct current speed regulating motor and the control system is provided with a control display screen 215, and because saliva is a complex biological fluid of the kit which is convenient for placing a sample bottle, the saliva contains various components such as cell fragments, protein aggregates, microorganisms and the like. The exosomes can be separated from these impurities by centrifugation. During centrifugation at lower rotational speeds, such as 300-500g, for 5-10 minutes, the saliva may be first freed from cells and large cell debris, which may have a high specific gravity, settle to the bottom of the centrifuge tube under the influence of centrifugal force, and then, increasing rotational speeds stepwise, such as 10,000-20,000g, for 30-60 minutes, may further remove smaller contaminant particles, allowing the exosomes to be enriched in the supernatant or pellet after centrifugation, depending on the size and density characteristics of the exosomes, which may help to obtain higher purity exosome samples for subsequent more accurate analysis and study, concentrating the exosomes, which may be of lower concentration in saliva, in some cases, not being conducive to direct detection and study. Centrifugation can be in the same place the exosome gathering, play concentrated effect, for example, when adopting ultracentrifugation 100,000-200,000g, the exosome can deposit the centrifuging tube bottom under the effect of centrifugal force to can concentrate the exosome that originally disperses in great volume saliva to less volume within range, the convenience is follow-up extraction, the analytical step, when being used for experiments such as proteomics, nucleic acid analysis, can satisfy the requirement of experiment to sample concentration, consequently the operator can set up the rotation speed of driving motor 211 through control display screen 215 according to the kind of sample, with this realization can be according to different sample kinds and demand and adjust to the rotational speed of looks adaptation.

In a further preferred embodiment of the present utility model, the auxiliary mechanism 22 includes an auxiliary shaft 221 and an auxiliary bevel gear 222, the middle of the driven wheel 213 is hollow, the auxiliary shaft 221 is disposed on the kit 1, the upper end of the auxiliary shaft passes through the hollow position of the driven wheel 213, the auxiliary bevel gear 222 is disposed at the upper end of the auxiliary shaft 221, the plurality of rotation mechanisms 23 each include a rotation bracket 231, a rotation shaft 232 and a rotation storage cylinder 233, the rotation brackets 231 are disposed at the upper ends of the driven wheels 213, the upper ends of the rotation brackets 231 are disposed in an inclined manner, the rotation shafts 232 are disposed at the upper ends of the rotation brackets 231 and face inwards, the rotation shafts 232 are rotatably connected with the rotation brackets 231, the rotation shafts 232 are sleeved with rotation gears 234 which are engaged with the auxiliary bevel gear 222 in a matching manner, and the rotation storage cylinders 233 are disposed at the upper ends of the rotation shafts 232;

When the sample bottle is required to be placed and stored, the reagent cover 12 is opened, then the required sample bottle is placed into the corresponding rotary storage cylinder 233, and the reagent cover 12 is closed;

When the centrifugal operation is needed to be carried out on the sample bottles in the reagent box 1, the follow-up better detection operation is realized, the driving motor 211 is controlled to start by operating on the control display screen 215, the driving motor 211 operates to drive the driving wheel 212 on the output end of the driving motor 211 to rotate, the driving wheel 212 rotates to drive the driven wheel 213 to rotate in the reagent box 1 through the cooperation sleeved by the belt 214, the driven wheel 213 rotates to drive the plurality of rotary brackets 231 to rotate, the plurality of rotary brackets 231 rotate to drive the corresponding rotary shafts 232 to rotate, the plurality of rotary shafts 232 rotate to drive the corresponding rotary gears 234 and the rotary storage cylinders to rotate circumferentially around the auxiliary bevel gear 222, the plurality of rotary gears 232 are connected with the corresponding rotary brackets 231 in a rotating mode, the plurality of rotary gears 234 rotate around the auxiliary bevel gear 222 and simultaneously, the plurality of rotary gears 234 also mesh with the auxiliary bevel gear 222, the plurality of rotary gears 232 mean that the rotary shafts 232 rotate, and finally the sample bottles in the plurality of rotary storage cylinders 233 rotate around the auxiliary bevel gear 222, the rotary storage cylinders 233 rotate, the sample bottles rotate around the auxiliary bevel gear 222, the sample bottles are separated from the sample bottles, the sample bottles can be separated from the sample bottles, and the sample bottles can be accurately and stored, and the sample bottles can be more accurately detected by the sample bottles can be separated.

In a further preferred embodiment of the present utility model, a storage groove 235 is provided in the rotary storage barrel 233, and the storage groove 235 is set to be of a size matching a conventional sample bottle;

through rotatory storage cylinder 233 inside be equipped with the storage tank 235 and this storage tank 235 is looks adaptation size setting with traditional sample bottle, can be convenient for sample bottle place.

In a further preferred embodiment of the present utility model, the inclination angle of the upper ends of the plurality of rotating brackets 231 is forty degrees;

The centrifugal rotation angle is generally 20-45 degrees, the sample is placed at a certain angle with the rotating shaft during the centrifugal process, the centrifugal force has components in the vertical direction and the horizontal direction, and when the centrifugal machine is started, the sample can be rapidly gathered to the outer side of the bottom of the centrifugal tube. The centrifugal device has the advantages that the centrifugal speed is relatively high, samples can be precipitated in a relatively short time, for example, in clinical examination, for rapid centrifugation of blood samples, the angle type centrifugal machine can separate blood cells from blood plasma in a few minutes, the design of the inclination angle is favorable for rapid sedimentation of blood cells to the bottom of the centrifugal tube, therefore, the inclination angle of the upper ends of the rotary supports 231 of the device is forty degrees, a plurality of sample bottles can be subjected to centrifugal operation and placement in forty degrees, the occupied space is small, cell fragments, bacterial precipitation and the like can be rapidly separated, and the centrifugal device is a relatively ideal choice.

The foregoing is merely exemplary of the present utility model and is not intended to limit the present utility model. Various modifications and variations of the present utility model will be apparent to those skilled in the art. Any modification, equivalent replacement, improvement, etc. which come within the spirit and principles of the utility model are to be included in the scope of the claims of the present utility model.

Claims (7)

1. A kit for facilitating placement of a sample bottle is characterized by comprising;

A kit (1) for storing sample bottles;

A centrifugal separation device (2) for carrying out centrifugal operation on the sample bottle is arranged in the kit (1);

The centrifugal separation device (2) comprises a driving mechanism (21), an auxiliary mechanism (22) and a rotating mechanism (23);

The utility model discloses a reagent kit, including reagent box (1), rotary mechanism (23), actuating mechanism (21) set up inside reagent box (1), rotary mechanism (23) are equipped with a plurality of, and a plurality of rotary mechanism (23) are the equidistance and encircle setting on actuating mechanism (21), complementary unit (22) set up inside reagent box (1), a plurality of rotary mechanism (23) are in the cooperation rotation state with complementary unit (22) respectively, reagent box (1) include reagent cavity (11) and reagent lid (12), reagent lid (12) set up on reagent cavity (11) and can open the setting.

2. A kit for facilitating sample bottle placement according to claim 1, wherein the driving mechanism (21) comprises a driving motor (211), a driving wheel (212), a driven wheel (213) and a belt (214), the driving motor (211) is arranged inside the kit (1), the driving wheel (212) is arranged at the output end of the driving motor (211), the driven wheel (213) is rotatably connected inside the kit (1) and is located beside the driving wheel (212), and the belt (214) is sleeved on the driving wheel (212) and the driven wheel (213).

3. The kit for facilitating sample bottle placement according to claim 1, wherein a control system for electrically connecting a driving motor (211) is arranged on the kit (1), the driving motor (211) is a direct-current speed-regulating motor, a control display screen (215) is arranged on the control system, and the control display screen (215) can operate and control the driving motor (211) to operate for touching the display screen.

4. A kit for facilitating placement of a sample bottle according to claim 2, wherein the auxiliary mechanism (22) comprises an auxiliary shaft (221) and an auxiliary bevel gear (222), the middle part of the driven wheel (213) is arranged in a hollow mode, the auxiliary shaft (221) is arranged on the kit (1) and the upper end of the auxiliary shaft passes through the hollow position of the driven wheel (213), and the auxiliary bevel gear (222) is arranged at the upper end of the auxiliary shaft (221).

5. The kit for facilitating sample bottle placement according to claim 1, wherein the plurality of rotating mechanisms (23) comprise rotating supports (231), rotating shafts (232) and rotating storage cylinders (233), the rotating supports (231) are arranged at the upper ends of driven wheels (213), the upper ends of the rotating supports (231) are obliquely arranged, the rotating shafts (232) are arranged at the upper ends of the rotating supports (231) and face inwards, the rotating shafts (232) are in rotary connection with the rotating supports (231), rotating gears (234) meshed with the auxiliary bevel gears (222) in a matched mode are sleeved on the rotating shafts (232), and the rotating storage cylinders (233) are arranged at the upper ends of the rotating shafts (232).

6. A kit for facilitating placement of sample vials as defined in claim 5, wherein a storage tank (235) is provided within the rotatable cartridge (233) and the storage tank (235) is sized to fit conventional sample vials.

7. A kit for facilitating placement of sample bottles as claimed in claim 5, wherein the upper ends of said plurality of rotary holders (231) are inclined at an angle of forty degrees.