Carrier fermentation tank
Technical Field
The utility model relates to the technical field of biological fermentation, in particular to a carrier fermentation tank.
Background
A fermenter refers to a container that provides a good environment for the operation of a specific biochemical process of microorganisms, is a device for deep cultivation of microorganisms in industrial fermentation, is also an essential core device in modern fermentation engineering, and is regarded as a heart of the fermentation industry and a bridge connecting raw materials and products. The fermenter is widely used in the fields including dairy products, beverages, bioengineering, pharmaceuticals, fine chemicals, food processing, environmental protection, etc. In the field of biological medicine, the fermenter is used in the production process of biological products such as recombinant proteins, vaccines, antibodies and the like, in the field of food processing, in the production and research and development of traditional fermented foods such as yoghurt, fermented bean curd, soy sauce and the like, in the field of chemical engineering, in the field of fermentation production of chemicals such as organic acids, amino acids and the like, and in the field of environmental protection, can be used for sewage treatment.
The fermentation tank has the working principle that the materials in the tank flow axially and radially by utilizing devices such as mechanical stirring or an air rotor, so that the materials are well mixed, and the solid matters in the liquid are kept in a suspended state, thereby being beneficial to the full contact of the solid matters and the nutrient matters and facilitating the nutrient absorption. Meanwhile, the requirement of maintaining the oxygen of the bacteria by introducing sterile air can be met, and the growth and fermentation of the aerobic bacteria can be realized.
Conventional fermentors typically employ batch fermentation, which has many problems that are difficult to solve:
First, batch fermentation requires frequent feeding, sterilization, cooling, washing, etc., which consume large amounts of materials and energy.
Secondly, the strains all need to undergo a delay period, a logarithmic growth phase, a stationary phase and a decay phase, so that the whole fermentation period is longer, the operation is complex, a large amount of manpower input is required for monitoring and managing the fermentation process, and the occupied time and manpower in a non-production phase (such as sterilization, cleaning and the like) are also more.
Again, conventional general fermentors may suffer from non-uniform mixing, poor mass transfer effects, etc. during fermentation, resulting in low fermentation efficiency, which can affect the yield and quality of the product.
Finally, the need to frequently open and close the fermenter during batch fermentation increases the risk of contamination, which, once it occurs, not only affects the quality of the current batch but also may cause continuous contamination of subsequent batches.
In addition, since it is difficult to precisely control fermentation conditions such as temperature, pH, dissolved oxygen, etc. in the conventional general-purpose fermenter, the yield of the product may be unstable and there is fluctuation, so that consistency between fermentation batches is difficult to ensure.
Therefore, in order to solve the above-mentioned problems, it is necessary to design a carrier fermenter.
It should be noted that the foregoing description of the background art is only for the purpose of providing a clear and complete description of the technical solution of the present utility model and is for the convenience of understanding by those skilled in the art, and is not to be construed as merely illustrative of the background art of the present utility model.
Disclosure of utility model
In order to overcome the defects in the prior art, the utility model aims to disclose a carrier fermentation tank which is used for solving the problems of low production efficiency, high production cost, unstable product quality, technical limitation and the like of the traditional general fermentation tank.
The utility model discloses a carrier fermentation tank which comprises a tank body and a stirring assembly, wherein a fermentation carrier for adsorbing bacteria is arranged in the tank body, a cooling water coil pipe for controlling the temperature in the tank body and an air distributor for controlling dissolved oxygen in the tank body are arranged on the tank body, the stirring assembly comprises a stirring motor arranged at the upper end of the tank body, the output end of the stirring motor is connected with a stirring shaft, the stirring shaft penetrates through the top of the tank body and is arranged in the tank body, a defoaming blade, a top blade, a middle blade and a bottom blade are sequentially arranged on the stirring shaft from top to bottom, the fermentation liquid in the tank body is respectively subjected to defoaming, the upper, middle and lower areas are respectively stirred, the uniformity of mixing of internal substances is ensured, the fermentation carrier is arranged between the tank body and the stirring assembly through a carrier frame, liquid flow generated during stirring can pass through the area where the fermentation carrier is located in a large range, nutrition and sterile air in the liquid flow can be fully contacted with the bacteria on the fermentation carrier, the lower end of the carrier frame is connected with the bottom of the tank body, and the air distributor is arranged at the lower end of the fermentation carrier and is used for providing sterile air.
The fermentation carrier and the corresponding carrier frames are at least two groups, the fermentation carrier is uniformly arranged on the circumference of the stirring shaft through the carrier frames, the carrier frames are vertically arranged by a plurality of carrier plates, and the distribution uniformity of internal thalli is improved.
The preferred technical proposal is that the number of the fermentation carriers and the corresponding carrier racks is five groups.
The preferred technical proposal is that the carrier frame is detachably arranged in the tank body, and is convenient to replace and clean.
The preferable technical proposal is that the top blade and the middle blade are tricuspid blades.
The preferable technical scheme is that the bottom blade is a six-flat blade turbine blade.
The lower end of the stirring shaft is rotationally connected with a bottom bearing, and the bottom bearing is arranged at the bottom of the tank body, so that the stability of the movement of the stirring shaft is improved.
The tank body comprises a cylinder body, openings at the upper end and the lower end of the cylinder body are respectively plugged through an upper sealing head and a lower sealing head, a cooling water coil pipe is arranged on the side wall of the cylinder body, and an air distributor is arranged at the lower sealing head.
The optimized technical scheme is that a manhole, a feed inlet, a feed supplementing port, an inoculation port and an exhaust port are arranged at the upper end socket, an inner coil cooling water inlet, an inner coil cooling water outlet, an outer coil cooling water inlet, an outer coil cooling water outlet, a sampling port and an electrode interface are arranged at the barrel, and an air inlet and a discharge port are arranged at the lower end socket.
The preferable technical scheme is that a support is arranged at the lower end of the tank body, so that the placement stability of the tank body is improved.
Due to the application of the technical scheme, compared with the prior art, the utility model has the following beneficial effects:
The utility model relates to a carrier fermentation tank, which is characterized in that a fermentation carrier is used for adsorbing thalli, the thalli is immobilized, the density of the thalli is improved, the stability and activity of the thalli can be improved, the fermentation period is reduced, the fermentation efficiency is improved, the mass transfer effect in fermentation liquid is improved by adopting a mode of adsorbing the thalli by the fermentation carrier, substrates and oxygen can be more effectively transferred to the surface of the thalli by stirring through continuously arranged paddles, the growth of the thalli and the accumulation of metabolites are promoted, the thalli can be prevented from losing in the fermentation process due to the immobilization of the thalli on the fermentation carrier, the concentration and purity of the thalli are improved, the carrier fermentation tank can more easily realize the accurate control of the fermentation process, such as the adjustment of key parameters of temperature, pH value, dissolved oxygen and the like, the stability and reproducibility of the fermentation are improved, the thalli is not easy to diffuse into the environment along with the flow of the fermentation liquid because the thalli is fixed on the fermentation carrier, the continuous high-density fermentation can be realized, the utilization rate of equipment is improved, the production cost is reduced, and the risk in the fermentation process is reduced.
Drawings
For a clearer description of embodiments of the utility model or of the prior art, the drawings which are used in the description of the embodiments or of the prior art will be briefly described, it being apparent that the drawings in the description below are some embodiments of the utility model and that other drawings can be obtained from them without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of a carrier fermenter according to the present utility model;
FIG. 2 is an internal top view of a carrier fermentor according to the present utility model.
In the drawings, 1, a tank body, 11, a cylinder body, 11a, an inner coil cooling water inlet, 11b, an inner coil cooling water outlet, 11c, an outer coil cooling water inlet, 11d, an outer coil cooling water outlet, 11e, a sampling port, 11f, an electrode interface, 12, an upper sealing head, 12a, a manhole, 12b, a feeding port, 12c, a feeding port, 12d, an inoculation port, 12e, an exhaust port, 13, a lower sealing head, 13a, an air inlet, 13b, a discharge port, 14, a support, 2, a stirring assembly, 21, a stirring motor, 22, a stirring shaft, 22a, defoaming paddles, 22b, a top paddle, 22c, an intermediate paddle, 22d, a bottom paddle, 22e, a bottom bearing, 3, a fermentation carrier, 4, a carrier frame, 5, a cooling water coil, 6 and an air distributor.
Detailed Description
Further advantages and effects of the present utility model will become apparent to those skilled in the art from the disclosure of the present utility model, which is described by the following specific examples.
It should be noted that the terms "first," "second," and the like in the description and the claims of the present application and the above figures are used for distinguishing between similar objects and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used may be interchanged where appropriate in order to describe embodiments of the application herein. Furthermore, the terms "comprises," "comprising," and "having," and their equivalents, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
In the present utility model, the terms "upper", "lower", "left", "right", "front", "rear", "top", "bottom", "inner", "outer", "middle", "vertical", "horizontal", "lateral", "longitudinal" and the like indicate an azimuth or a positional relationship based on that shown in the drawings. These terms are only used to better describe the present utility model and its embodiments and are not intended to limit the scope of the indicated devices, elements or components to the particular orientations or to configure and operate in the particular orientations.
Also, some of the terms described above may be used to indicate other meanings in addition to orientation or positional relationships, for example, the term "upper" may also be used to indicate some sort of attachment or connection in some cases. The specific meaning of these terms in the present utility model will be understood by those of ordinary skill in the art according to the specific circumstances.
Furthermore, the terms "mounted," "configured," "provided," "connected," "sleeved," and "attached" are to be construed broadly. For example, "connected" may be in a fixed connection, a removable connection, or a unitary construction, may be in a mechanical connection, or an electrical connection, may be directly connected, or indirectly connected via an intermediate medium, or may be in internal communication between two devices, elements, or components. For another example, "fit" may be a complete fit or a partial fit. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.
It should be noted that, without conflict, the embodiments of the present application and features of the embodiments may be combined with each other. The application will be described in detail below with reference to the drawings in connection with embodiments.
Examples:
As shown in fig. 1 and 2, the present utility model discloses a carrier fermentation tank, which comprises a tank body 1, a stirring assembly 2, a fermentation carrier 3, a carrier frame 4, a cooling water coil 5 and an air distributor 6, wherein the main components of the present utility model are specifically described below:
As shown in fig. 1 and 2, the tank 1 comprises a cylinder 11, and openings at the upper end and the lower end of the cylinder 11 are respectively plugged through an upper sealing head 12 and a lower sealing head 13, so that the tank 1 is convenient to disassemble, assemble and clean.
As shown in fig. 1 and 2, the stirring assembly 2 comprises a stirring motor 21 arranged at the upper end of the tank body 1, the output end of the stirring motor 21 is connected with a stirring shaft 22 through a coupling, the stirring shaft 22 penetrates through the top of the tank body 1 and is arranged in the tank body, the stirring shaft 22 is sequentially provided with a defoaming blade 22a, a top blade 22b, a middle blade 22c and a bottom blade 22d from top to bottom, fermentation liquor in the tank body 1 is respectively defoamed, and three areas in the upper, middle and lower directions are stirred through the layered blades, so that uniformity of mixing of internal substances is ensured, and foam generated in the tank is eliminated by the defoaming blade 22 a.
As shown in fig. 1 and 2, the fermentation carrier 3 is arranged between the tank 1 and the stirring assembly 2 through the carrier frame 4, the lower end of the carrier frame 4 is connected with the lower sealing head 13, the number of the fermentation carriers 3 and the corresponding carrier frames 4 is five, the fermentation carriers 3 are uniformly arranged along the circumferential direction of the stirring shaft 22 through the carrier frames 4, the carrier frames 4 are vertically arranged by a plurality of carrier plates, so that the bacterial loss on the fermentation carrier 3 can be effectively avoided, and the fermentation efficiency is improved.
As shown in fig. 1 and 2, the cooling water coil 5 is spirally disposed on the inner and outer side walls of the cylinder 11, and the ability to adjust the temperature in the tank 1 is increased.
As shown in fig. 1 and 2, the air distributor 6 is arranged at the lower end closure 13 at the lower end of the fermentation carrier 3, so that the conveyed sterile air enters the tank 1 from the lower end of the fermentation carrier 3, the comprehensive contact between the bacteria on the fermentation carrier 3 and the sterile air is ensured, and the growth of the bacteria and the accumulation of metabolites are promoted.
As shown in fig. 1 and 2, in order to ensure that the energy consumption of the carrier fermentation tank is reduced, the top blade 22b and the middle blade 22c adopt tricuspid blades, and for the upper layer in the tank body 1, the tricuspid blades can effectively reduce the energy consumption while ensuring the mixing effect.
As shown in fig. 1 and 2, to enhance the mixing effect, the bottom blade 22d employs a six-blade turbine blade, which produces a stronger shearing force during the stirring process, so that the substrate and oxygen can be more effectively transferred to the surface of the cell.
As shown in fig. 1 and 2, in order to improve the stability of the movement of the stirring assembly 2, the lower end of the stirring shaft 22 is rotatably connected with a bottom bearing 22e, the bottom bearing 22e is disposed at the bottom of the tank 1, and the stirring shaft 22 is prevented from swinging during operation by the bottom bearing 22 e.
As shown in fig. 1 and 2, in order to facilitate cleaning of the tank 1, the lower end of the carrier frame 4 is detachably connected with the lower end socket 13, and the carrier frame 4 and the fermentation carrier 3 thereon can be taken out for replacement and cleaning during cleaning.
As shown in fig. 1 and 2, in order to improve the convenience of using the carrier fermenter, a manhole 12a, a feed inlet 12b, a feed supplement inlet 12c, a inoculation inlet 12d and an exhaust outlet 12e are provided at the upper closure 12, raw materials added from the upper end are more easily mixed with internal materials, while gas is light, exhaust gas is discharged from above to avoid remaining in the tank, an inner coil cooling water inlet 11a, an inner coil cooling water outlet 11b, an outer coil cooling water inlet 11c, an outer coil cooling water outlet 11d, a sampling inlet 11e and an electrode interface 11f are provided at the cylinder 11, which is more advantageous for temperature control and standard sample collection, and an air inlet 13a and an exhaust outlet 13b are provided at the lower closure 13.
As shown in fig. 1 and 2, in order to ensure the stability of the carrier fermentation tank, a support 14 is provided at the lower end of the tank body 1, and the periphery of the tank body is supported by the support 14 to prevent the tank body 1 from toppling.
Finally, it should be noted that the foregoing description is only a preferred embodiment of the present utility model, and although the present utility model has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the technical solutions described in the foregoing embodiments, or equivalents may be substituted for some of the technical features thereof, and any modifications, equivalents, improvements or changes made within the spirit and principles of the present utility model should be included in the scope of the present utility model.