CN223357643U - A carrier fermentation tank - Google Patents

A carrier fermentation tank

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Publication number
CN223357643U
CN223357643U CN202422593577.XU CN202422593577U CN223357643U CN 223357643 U CN223357643 U CN 223357643U CN 202422593577 U CN202422593577 U CN 202422593577U CN 223357643 U CN223357643 U CN 223357643U
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China
Prior art keywords
carrier
tank body
fermentation
stirring
cooling water
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CN202422593577.XU
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Chinese (zh)
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张雪阳
许健锋
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Wuxi Rongfeng Bioengineering Co ltd
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Wuxi Rongfeng Bioengineering Co ltd
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Abstract

一种载体发酵罐,包括罐体和搅拌组件,罐体内设发酵载体,罐体上设冷却水盘管和空气分布器;搅拌组件包括搅拌电机,搅拌电机的输出端连接搅拌轴,搅拌轴穿过罐体的顶部设置在罐体的内部;搅拌轴从上至下依次设有消泡桨叶、顶部桨叶、中间桨叶和底部桨叶,发酵载体通过载体架设置在罐体与搅拌组件之间;载体架下端与罐体底部连接,空气分布器设置在发酵载体的下端。本实用新型通过发酵载体吸附菌体,提高菌体的密度,从而降低发酵周期,提高发酵效率;通过连续设置的桨叶搅拌,使底物和氧气能够有效地传递到菌体表面;通过冷却水盘管、空气分布器和在电极接口内设置电极,可以更容易地实现发酵过程的精确控制,降低生产成本,减少发酵过程中的污染风险。

A carrier fermentation tank includes a tank body and a stirring assembly, wherein a fermentation carrier is disposed within the tank body, and a cooling water coil and an air distributor are disposed on the tank body; the stirring assembly includes a stirring motor, the output end of which is connected to a stirring shaft, which passes through the top of the tank body and is disposed within the tank body; the stirring shaft is sequentially provided with a defoaming blade, a top blade, a middle blade, and a bottom blade from top to bottom, and the fermentation carrier is disposed between the tank body and the stirring assembly via a carrier frame; the lower end of the carrier frame is connected to the bottom of the tank body, and the air distributor is disposed at the lower end of the fermentation carrier. The utility model absorbs bacteria through the fermentation carrier, thereby increasing the density of the bacteria, thereby reducing the fermentation cycle and improving the fermentation efficiency; through continuous stirring with blades, substrate and oxygen can be effectively transferred to the surface of the bacteria; through the cooling water coil, the air distributor, and the electrodes disposed within the electrode interface, precise control of the fermentation process can be achieved more easily, reducing production costs and minimizing the risk of contamination during the fermentation process.

Description

Carrier fermentation tank
Technical Field
The utility model relates to the technical field of biological fermentation, in particular to a carrier fermentation tank.
Background
A fermenter refers to a container that provides a good environment for the operation of a specific biochemical process of microorganisms, is a device for deep cultivation of microorganisms in industrial fermentation, is also an essential core device in modern fermentation engineering, and is regarded as a heart of the fermentation industry and a bridge connecting raw materials and products. The fermenter is widely used in the fields including dairy products, beverages, bioengineering, pharmaceuticals, fine chemicals, food processing, environmental protection, etc. In the field of biological medicine, the fermenter is used in the production process of biological products such as recombinant proteins, vaccines, antibodies and the like, in the field of food processing, in the production and research and development of traditional fermented foods such as yoghurt, fermented bean curd, soy sauce and the like, in the field of chemical engineering, in the field of fermentation production of chemicals such as organic acids, amino acids and the like, and in the field of environmental protection, can be used for sewage treatment.
The fermentation tank has the working principle that the materials in the tank flow axially and radially by utilizing devices such as mechanical stirring or an air rotor, so that the materials are well mixed, and the solid matters in the liquid are kept in a suspended state, thereby being beneficial to the full contact of the solid matters and the nutrient matters and facilitating the nutrient absorption. Meanwhile, the requirement of maintaining the oxygen of the bacteria by introducing sterile air can be met, and the growth and fermentation of the aerobic bacteria can be realized.
Conventional fermentors typically employ batch fermentation, which has many problems that are difficult to solve:
First, batch fermentation requires frequent feeding, sterilization, cooling, washing, etc., which consume large amounts of materials and energy.
Secondly, the strains all need to undergo a delay period, a logarithmic growth phase, a stationary phase and a decay phase, so that the whole fermentation period is longer, the operation is complex, a large amount of manpower input is required for monitoring and managing the fermentation process, and the occupied time and manpower in a non-production phase (such as sterilization, cleaning and the like) are also more.
Again, conventional general fermentors may suffer from non-uniform mixing, poor mass transfer effects, etc. during fermentation, resulting in low fermentation efficiency, which can affect the yield and quality of the product.
Finally, the need to frequently open and close the fermenter during batch fermentation increases the risk of contamination, which, once it occurs, not only affects the quality of the current batch but also may cause continuous contamination of subsequent batches.
In addition, since it is difficult to precisely control fermentation conditions such as temperature, pH, dissolved oxygen, etc. in the conventional general-purpose fermenter, the yield of the product may be unstable and there is fluctuation, so that consistency between fermentation batches is difficult to ensure.
Therefore, in order to solve the above-mentioned problems, it is necessary to design a carrier fermenter.
It should be noted that the foregoing description of the background art is only for the purpose of providing a clear and complete description of the technical solution of the present utility model and is for the convenience of understanding by those skilled in the art, and is not to be construed as merely illustrative of the background art of the present utility model.
Disclosure of utility model
In order to overcome the defects in the prior art, the utility model aims to disclose a carrier fermentation tank which is used for solving the problems of low production efficiency, high production cost, unstable product quality, technical limitation and the like of the traditional general fermentation tank.
The utility model discloses a carrier fermentation tank which comprises a tank body and a stirring assembly, wherein a fermentation carrier for adsorbing bacteria is arranged in the tank body, a cooling water coil pipe for controlling the temperature in the tank body and an air distributor for controlling dissolved oxygen in the tank body are arranged on the tank body, the stirring assembly comprises a stirring motor arranged at the upper end of the tank body, the output end of the stirring motor is connected with a stirring shaft, the stirring shaft penetrates through the top of the tank body and is arranged in the tank body, a defoaming blade, a top blade, a middle blade and a bottom blade are sequentially arranged on the stirring shaft from top to bottom, the fermentation liquid in the tank body is respectively subjected to defoaming, the upper, middle and lower areas are respectively stirred, the uniformity of mixing of internal substances is ensured, the fermentation carrier is arranged between the tank body and the stirring assembly through a carrier frame, liquid flow generated during stirring can pass through the area where the fermentation carrier is located in a large range, nutrition and sterile air in the liquid flow can be fully contacted with the bacteria on the fermentation carrier, the lower end of the carrier frame is connected with the bottom of the tank body, and the air distributor is arranged at the lower end of the fermentation carrier and is used for providing sterile air.
The fermentation carrier and the corresponding carrier frames are at least two groups, the fermentation carrier is uniformly arranged on the circumference of the stirring shaft through the carrier frames, the carrier frames are vertically arranged by a plurality of carrier plates, and the distribution uniformity of internal thalli is improved.
The preferred technical proposal is that the number of the fermentation carriers and the corresponding carrier racks is five groups.
The preferred technical proposal is that the carrier frame is detachably arranged in the tank body, and is convenient to replace and clean.
The preferable technical proposal is that the top blade and the middle blade are tricuspid blades.
The preferable technical scheme is that the bottom blade is a six-flat blade turbine blade.
The lower end of the stirring shaft is rotationally connected with a bottom bearing, and the bottom bearing is arranged at the bottom of the tank body, so that the stability of the movement of the stirring shaft is improved.
The tank body comprises a cylinder body, openings at the upper end and the lower end of the cylinder body are respectively plugged through an upper sealing head and a lower sealing head, a cooling water coil pipe is arranged on the side wall of the cylinder body, and an air distributor is arranged at the lower sealing head.
The optimized technical scheme is that a manhole, a feed inlet, a feed supplementing port, an inoculation port and an exhaust port are arranged at the upper end socket, an inner coil cooling water inlet, an inner coil cooling water outlet, an outer coil cooling water inlet, an outer coil cooling water outlet, a sampling port and an electrode interface are arranged at the barrel, and an air inlet and a discharge port are arranged at the lower end socket.
The preferable technical scheme is that a support is arranged at the lower end of the tank body, so that the placement stability of the tank body is improved.
Due to the application of the technical scheme, compared with the prior art, the utility model has the following beneficial effects:
The utility model relates to a carrier fermentation tank, which is characterized in that a fermentation carrier is used for adsorbing thalli, the thalli is immobilized, the density of the thalli is improved, the stability and activity of the thalli can be improved, the fermentation period is reduced, the fermentation efficiency is improved, the mass transfer effect in fermentation liquid is improved by adopting a mode of adsorbing the thalli by the fermentation carrier, substrates and oxygen can be more effectively transferred to the surface of the thalli by stirring through continuously arranged paddles, the growth of the thalli and the accumulation of metabolites are promoted, the thalli can be prevented from losing in the fermentation process due to the immobilization of the thalli on the fermentation carrier, the concentration and purity of the thalli are improved, the carrier fermentation tank can more easily realize the accurate control of the fermentation process, such as the adjustment of key parameters of temperature, pH value, dissolved oxygen and the like, the stability and reproducibility of the fermentation are improved, the thalli is not easy to diffuse into the environment along with the flow of the fermentation liquid because the thalli is fixed on the fermentation carrier, the continuous high-density fermentation can be realized, the utilization rate of equipment is improved, the production cost is reduced, and the risk in the fermentation process is reduced.
Drawings
For a clearer description of embodiments of the utility model or of the prior art, the drawings which are used in the description of the embodiments or of the prior art will be briefly described, it being apparent that the drawings in the description below are some embodiments of the utility model and that other drawings can be obtained from them without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of a carrier fermenter according to the present utility model;
FIG. 2 is an internal top view of a carrier fermentor according to the present utility model.
In the drawings, 1, a tank body, 11, a cylinder body, 11a, an inner coil cooling water inlet, 11b, an inner coil cooling water outlet, 11c, an outer coil cooling water inlet, 11d, an outer coil cooling water outlet, 11e, a sampling port, 11f, an electrode interface, 12, an upper sealing head, 12a, a manhole, 12b, a feeding port, 12c, a feeding port, 12d, an inoculation port, 12e, an exhaust port, 13, a lower sealing head, 13a, an air inlet, 13b, a discharge port, 14, a support, 2, a stirring assembly, 21, a stirring motor, 22, a stirring shaft, 22a, defoaming paddles, 22b, a top paddle, 22c, an intermediate paddle, 22d, a bottom paddle, 22e, a bottom bearing, 3, a fermentation carrier, 4, a carrier frame, 5, a cooling water coil, 6 and an air distributor.
Detailed Description
Further advantages and effects of the present utility model will become apparent to those skilled in the art from the disclosure of the present utility model, which is described by the following specific examples.
It should be noted that the terms "first," "second," and the like in the description and the claims of the present application and the above figures are used for distinguishing between similar objects and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used may be interchanged where appropriate in order to describe embodiments of the application herein. Furthermore, the terms "comprises," "comprising," and "having," and their equivalents, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
In the present utility model, the terms "upper", "lower", "left", "right", "front", "rear", "top", "bottom", "inner", "outer", "middle", "vertical", "horizontal", "lateral", "longitudinal" and the like indicate an azimuth or a positional relationship based on that shown in the drawings. These terms are only used to better describe the present utility model and its embodiments and are not intended to limit the scope of the indicated devices, elements or components to the particular orientations or to configure and operate in the particular orientations.
Also, some of the terms described above may be used to indicate other meanings in addition to orientation or positional relationships, for example, the term "upper" may also be used to indicate some sort of attachment or connection in some cases. The specific meaning of these terms in the present utility model will be understood by those of ordinary skill in the art according to the specific circumstances.
Furthermore, the terms "mounted," "configured," "provided," "connected," "sleeved," and "attached" are to be construed broadly. For example, "connected" may be in a fixed connection, a removable connection, or a unitary construction, may be in a mechanical connection, or an electrical connection, may be directly connected, or indirectly connected via an intermediate medium, or may be in internal communication between two devices, elements, or components. For another example, "fit" may be a complete fit or a partial fit. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.
It should be noted that, without conflict, the embodiments of the present application and features of the embodiments may be combined with each other. The application will be described in detail below with reference to the drawings in connection with embodiments.
Examples:
As shown in fig. 1 and 2, the present utility model discloses a carrier fermentation tank, which comprises a tank body 1, a stirring assembly 2, a fermentation carrier 3, a carrier frame 4, a cooling water coil 5 and an air distributor 6, wherein the main components of the present utility model are specifically described below:
As shown in fig. 1 and 2, the tank 1 comprises a cylinder 11, and openings at the upper end and the lower end of the cylinder 11 are respectively plugged through an upper sealing head 12 and a lower sealing head 13, so that the tank 1 is convenient to disassemble, assemble and clean.
As shown in fig. 1 and 2, the stirring assembly 2 comprises a stirring motor 21 arranged at the upper end of the tank body 1, the output end of the stirring motor 21 is connected with a stirring shaft 22 through a coupling, the stirring shaft 22 penetrates through the top of the tank body 1 and is arranged in the tank body, the stirring shaft 22 is sequentially provided with a defoaming blade 22a, a top blade 22b, a middle blade 22c and a bottom blade 22d from top to bottom, fermentation liquor in the tank body 1 is respectively defoamed, and three areas in the upper, middle and lower directions are stirred through the layered blades, so that uniformity of mixing of internal substances is ensured, and foam generated in the tank is eliminated by the defoaming blade 22 a.
As shown in fig. 1 and 2, the fermentation carrier 3 is arranged between the tank 1 and the stirring assembly 2 through the carrier frame 4, the lower end of the carrier frame 4 is connected with the lower sealing head 13, the number of the fermentation carriers 3 and the corresponding carrier frames 4 is five, the fermentation carriers 3 are uniformly arranged along the circumferential direction of the stirring shaft 22 through the carrier frames 4, the carrier frames 4 are vertically arranged by a plurality of carrier plates, so that the bacterial loss on the fermentation carrier 3 can be effectively avoided, and the fermentation efficiency is improved.
As shown in fig. 1 and 2, the cooling water coil 5 is spirally disposed on the inner and outer side walls of the cylinder 11, and the ability to adjust the temperature in the tank 1 is increased.
As shown in fig. 1 and 2, the air distributor 6 is arranged at the lower end closure 13 at the lower end of the fermentation carrier 3, so that the conveyed sterile air enters the tank 1 from the lower end of the fermentation carrier 3, the comprehensive contact between the bacteria on the fermentation carrier 3 and the sterile air is ensured, and the growth of the bacteria and the accumulation of metabolites are promoted.
As shown in fig. 1 and 2, in order to ensure that the energy consumption of the carrier fermentation tank is reduced, the top blade 22b and the middle blade 22c adopt tricuspid blades, and for the upper layer in the tank body 1, the tricuspid blades can effectively reduce the energy consumption while ensuring the mixing effect.
As shown in fig. 1 and 2, to enhance the mixing effect, the bottom blade 22d employs a six-blade turbine blade, which produces a stronger shearing force during the stirring process, so that the substrate and oxygen can be more effectively transferred to the surface of the cell.
As shown in fig. 1 and 2, in order to improve the stability of the movement of the stirring assembly 2, the lower end of the stirring shaft 22 is rotatably connected with a bottom bearing 22e, the bottom bearing 22e is disposed at the bottom of the tank 1, and the stirring shaft 22 is prevented from swinging during operation by the bottom bearing 22 e.
As shown in fig. 1 and 2, in order to facilitate cleaning of the tank 1, the lower end of the carrier frame 4 is detachably connected with the lower end socket 13, and the carrier frame 4 and the fermentation carrier 3 thereon can be taken out for replacement and cleaning during cleaning.
As shown in fig. 1 and 2, in order to improve the convenience of using the carrier fermenter, a manhole 12a, a feed inlet 12b, a feed supplement inlet 12c, a inoculation inlet 12d and an exhaust outlet 12e are provided at the upper closure 12, raw materials added from the upper end are more easily mixed with internal materials, while gas is light, exhaust gas is discharged from above to avoid remaining in the tank, an inner coil cooling water inlet 11a, an inner coil cooling water outlet 11b, an outer coil cooling water inlet 11c, an outer coil cooling water outlet 11d, a sampling inlet 11e and an electrode interface 11f are provided at the cylinder 11, which is more advantageous for temperature control and standard sample collection, and an air inlet 13a and an exhaust outlet 13b are provided at the lower closure 13.
As shown in fig. 1 and 2, in order to ensure the stability of the carrier fermentation tank, a support 14 is provided at the lower end of the tank body 1, and the periphery of the tank body is supported by the support 14 to prevent the tank body 1 from toppling.
Finally, it should be noted that the foregoing description is only a preferred embodiment of the present utility model, and although the present utility model has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the technical solutions described in the foregoing embodiments, or equivalents may be substituted for some of the technical features thereof, and any modifications, equivalents, improvements or changes made within the spirit and principles of the present utility model should be included in the scope of the present utility model.

Claims (10)

1. A carrier fermentation tank comprises a tank body (1) and a stirring assembly (2), wherein a fermentation carrier (3) is arranged in the tank body (1), and is characterized in that a cooling water coil pipe (5) and an air distributor (6) are arranged on the tank body (1), the stirring assembly (2) comprises a stirring motor (21) arranged at the upper end of the tank body (1), the output end of the stirring motor (21) is connected with a stirring shaft (22), the stirring shaft (22) penetrates through the top of the tank body (1) and is arranged in the tank body, defoaming paddles (22 a), top paddles (22 b), middle paddles (22 c) and bottom paddles (22 d) are sequentially arranged on the stirring shaft (22) from top to bottom, the fermentation carrier (3) is arranged between the tank body (1) and the stirring assembly (2) through a carrier frame (4), the lower end of the carrier frame (4) is connected with the bottom of the tank body (1), and the air distributor (6) is arranged at the lower end of the fermentation carrier (3).
2. A carrier fermenter according to claim 1, wherein the number of the fermenting carriers (3) and the corresponding carrier holders (4) is at least two, the fermenting carriers (3) are uniformly arranged in the circumferential direction of the stirring shaft (22) through the carrier holders (4), and the carrier holders (4) are composed of a plurality of carrier plates vertically arranged.
3. A carrier fermenter according to claim 2, wherein the number of fermenting carriers (3) and corresponding carrier holders (4) is five.
4. A carrier fermenter according to claim 3, wherein the carrier frame (4) is detachably arranged in the tank body (1).
5. A carrier fermenter according to claim 1, wherein the top blade (22 b) and the middle blade (22 c) are tricuspid blades.
6. A carrier fermenter according to claim 5, wherein the bottom blades (22 d) are six-bladed turbine paddles.
7. A carrier fermenter according to claim 6, wherein the lower end of the stirring shaft (22) is rotatably connected with a bottom bearing (22 e), the bottom bearing (22 e) being arranged at the bottom of the tank body (1).
8. The carrier fermentation tank of claim 1, wherein the tank body (1) comprises a cylinder body (11), openings at the upper end and the lower end of the cylinder body (11) are respectively plugged through an upper sealing head (12) and a lower sealing head (13), the cooling water coil (5) is arranged on the inner side wall and the outer side wall of the cylinder body (11), and the air distributor (6) is arranged at the lower sealing head (13).
9. The carrier fermentation tank of claim 8, wherein a manhole (12 a), a feed inlet (12 b), a feed supplement inlet (12 c), an inoculation port (12 d) and an exhaust port (12 e) are arranged at the upper end socket (12), an inner coil cooling water inlet (11 a), an inner coil cooling water outlet (11 b), an outer coil cooling water inlet (11 c), an outer coil cooling water outlet (11 d), a sampling port (11 e) and an electrode interface (11 f) are arranged at the barrel (11), and an air inlet (13 a) and a discharge port (13 b) are arranged at the lower end socket (13).
10. A carrier fermenter according to claim 1, wherein the lower end of the tank body (1) is provided with a support (14).
CN202422593577.XU 2024-10-25 2024-10-25 A carrier fermentation tank Active CN223357643U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202422593577.XU CN223357643U (en) 2024-10-25 2024-10-25 A carrier fermentation tank

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202422593577.XU CN223357643U (en) 2024-10-25 2024-10-25 A carrier fermentation tank

Publications (1)

Publication Number Publication Date
CN223357643U true CN223357643U (en) 2025-09-19

Family

ID=97033891

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202422593577.XU Active CN223357643U (en) 2024-10-25 2024-10-25 A carrier fermentation tank

Country Status (1)

Country Link
CN (1) CN223357643U (en)

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