CN223197038U - Microcarrier crosslinked cleaning system - Google Patents
Microcarrier crosslinked cleaning systemInfo
- Publication number
- CN223197038U CN223197038U CN202422491558.6U CN202422491558U CN223197038U CN 223197038 U CN223197038 U CN 223197038U CN 202422491558 U CN202422491558 U CN 202422491558U CN 223197038 U CN223197038 U CN 223197038U
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- Prior art keywords
- cross
- microcarrier
- linking
- filter
- microcarriers
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Abstract
本申请提供一种微载体交联清洗系统，该系统通过过滤器内加入微载体的方式，能够便于微载体与交联试剂、清洗液等试剂的分离，使微载体交联免受生产试剂的干扰；通过搅拌装置的搅拌能够使微载体悬浮于交联剂中，保证微载体交联的均一性；通过搅拌装置的搅拌能够使交联微载体悬浮于清洗液中，减少了交联剂在交联微载体中的残留，使残留量在可控范围内。该系统中，搅拌装置设置在罐体内部而不是设置在过滤器内部，保证了微载体未交联前的物理稳定性，同时保证了微载体不会与搅拌装置接触，进而微载体不会破碎，提高了微载体的质量。该系统能够连续实现微载体前处理、微载体交联、交联微载体清洗及收集的连续生产。
The present application provides a microcarrier cross-linking and cleaning system. The system can facilitate the separation of microcarriers from cross-linking reagents, cleaning solutions and other reagents by adding microcarriers into a filter, thereby protecting the cross-linking of microcarriers from interference from production reagents; the stirring of the stirring device can suspend the microcarriers in the cross-linking agent, thereby ensuring the uniformity of the cross-linking of the microcarriers; the stirring of the stirring device can suspend the cross-linked microcarriers in the cleaning solution, thereby reducing the residue of the cross-linking agent in the cross-linked microcarriers and keeping the residue within a controllable range. In this system, the stirring device is arranged inside the tank body instead of inside the filter, thereby ensuring the physical stability of the microcarriers before cross-linking, and at the same time ensuring that the microcarriers will not come into contact with the stirring device, thereby preventing the microcarriers from being broken, thereby improving the quality of the microcarriers. The system can continuously realize the continuous production of microcarrier pretreatment, microcarrier cross-linking, cross-linked microcarrier cleaning and collection.
Description
Technical Field
The utility model belongs to the technical field of cell culture, and relates to a microcarrier cross-linking cleaning system.
Background
Microcarrier culture technology is an emerging large-scale cell culture technology, which uses tiny particles of microcarriers as cell carriers, and makes cells propagate into a monolayer on the surface or inside of the carriers by stirring and suspending in a culture solution. The microcarrier culture technology is widely applied to the production of vaccines, genetic engineering products and the like, is currently recognized as the most promising animal cell large-scale culture technology, has the advantages of suspension culture and adherence culture, and is easy to amplify.
The stability and mechanical strength of the uncrosslinked microcarrier are low and cannot be applied directly, so that it is often modified by crosslinking to enhance its physical properties. The crosslinking methods generally used are a physical crosslinking method and a chemical crosslinking method, but the degree of crosslinking by the physical crosslinking method is low and nonuniform, and therefore, the crosslinking method generally used is a chemical crosslinking method. Currently, chemical cross-linking agents commonly used include glutaraldehyde, formaldehyde, ethylene glycol, genipin, and the like, and these cross-linking agents are generally cytotoxic, resulting in a decrease in the viability of normal cells, and therefore, the chemically cross-linked microcarriers need to be sufficiently cleaned to control the residual amount of the cross-linking agent, and thus used for cell culture.
In the microcarrier preparation, crosslinking and cleaning processes, the conventional device equipment is usually small equipment in an experimental stage, continuous production cannot be realized, and the prepared microcarrier is nonuniform.
Disclosure of utility model
The utility model aims to provide a microcarrier cross-linking cleaning system so as to solve the problem that the conventional device cannot realize continuous production.
In order to achieve the above purpose, the present utility model adopts the following technical scheme:
The utility model provides a microcarrier cross-linking cleaning system which comprises a bracket and a fixing frame positioned above the bracket, wherein a motor is arranged in the bracket, a tank body is fixedly arranged on the fixing frame, a stirring device is arranged in the tank body, the output end of the motor is connected with the stirring device, a liquid outlet pipe is arranged at the bottom of the tank body, a sealing cover is arranged at the top of the tank body, a feeding port and a filter which are communicated are arranged at two opposite sides of the sealing cover, and the filter is positioned in the tank body and above the stirring device.
Preferably, the filter is a funnel-shaped filter.
Preferably, the filter comprises a filter screen and a filter membrane limiting part positioned in the filter screen, wherein a filter membrane is placed in the filter membrane limiting part, a limiting plate and a clamping groove are arranged at the top of the filter screen, the limiting plate is detachably arranged on the sealing cover, and the filter membrane limiting part is rotationally clamped in the clamping groove.
Preferably, the stirring device comprises a stirring shaft and stirring blades which are connected, and the stirring shaft is connected with the output end of the motor.
Preferably, the sealing cover is provided with at least one pair of the feed inlet and the filter.
Preferably, the top of the sealing cover is also provided with a liquid inlet which is communicated with the inside of the tank body.
Preferably, a liquid outlet valve is arranged on the liquid outlet pipe.
Preferably, a support column is arranged between the support and the fixing frame.
Preferably, the system further comprises a housing, wherein the housing is positioned outside the bracket and the tank body, and the sealing cover and the feed inlet are exposed at the top of the housing.
Preferably, the liquid outlet valve on the liquid outlet pipe is exposed out of the shell.
The utility model has the following beneficial effects:
(1) The microcarrier is conveniently separated from the crosslinking reagent, the cleaning liquid and other reagents by adding the microcarrier into the filter, so that the microcarrier is crosslinked and prevented from being interfered by the production reagent.
(2) The pore diameter of the filter membrane is smaller than the standard diameter of the microcarrier, so that the microcarrier fragments with the diameter smaller than the standard diameter of the microcarrier can be filtered conveniently, and the uniformity of the microcarrier is ensured.
(3) The stirring device can suspend the microcarrier in the crosslinking agent to ensure the uniformity of the crosslinking of the microcarrier, and the stirring device can suspend the crosslinking microcarrier in the cleaning liquid to reduce the residue of the crosslinking agent in the crosslinking microcarrier and ensure the residue amount to be in a controllable range.
(4) The stirring device is arranged in the tank body instead of the filter, so that the physical stability of the microcarrier before crosslinking is ensured, the microcarrier is ensured not to be contacted with the stirring device, the microcarrier is further prevented from being broken, and the quality of the microcarrier is improved.
(5) The feed inlet and the liquid inlet are both funnel-shaped, so that microcarriers and cross-linking agents can conveniently enter the filter, and the filter is funnel-shaped, so that microcarriers can conveniently settle and collect.
(6) The microcarrier cross-linking cleaning system can continuously realize the continuous production of microcarrier pretreatment, microcarrier cross-linking, cross-linking microcarrier cleaning and collection.
Drawings
FIG. 1 is a schematic perspective view of a microcarrier cross-linking cleaning system according to an embodiment of the present application;
FIG. 2 is a cross-sectional view of a microcarrier cross-linked cleaning system provided in an embodiment of the application;
FIG. 3 is a schematic perspective view of a filter according to an embodiment of the present application;
The symbols represent:
1-bracket, 2-fixing frame, 3-motor, 4-tank, 5-stirring device, 6-liquid outlet pipe, 7-sealing cover, 8-feed inlet, 9-filter, 10-liquid outlet valve, 11-liquid inlet, 12-support column and 13-shell;
501-stirring shaft and 502-stirring blade;
901-a filter screen, 902-a filter membrane limiting piece, 903-a limiting plate and 904-a clamping groove.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
The embodiment of the application provides a microcarrier cross-linking cleaning system, which comprises a bracket 1, a fixing frame 2, a tank body 4, a motor 3 and other components, as shown in figures 1 and 2. The bracket 1 is positioned at the bottom end and is used for supporting the fixing frame 2, the tank body 4 and the like. The motor 3 is arranged inside the bracket 1, and the motor 3 is used for driving the stirring device 5 inside the tank body 4 to stir.
The fixing frame 2 is arranged above the bracket 1 and is arranged at the top of the bracket 1 through the supporting column 12, and the top of the fixing frame 2 is provided with a tank body 4. In the embodiment of the present application, the supporting column 12 is a damping supporting column, so as to prevent the tank 4 from shaking during the stirring process, and improve the stability of the system.
The top of the tank body 4 is provided with a sealing cover 7, and the sealing cover 7 is used for sealing the top of the tank body 4 and preventing liquid in the tank body 4 from splashing. The opposite sides of the sealing cover 7 are provided with a feed inlet 8 and a filter 9 which are communicated, the filter 9 is positioned in the tank body 4, and the feed inlet 8 is positioned outside the tank body 4. Microcarriers enter the filter 9 through the feed inlet 8, and microcarriers, microcarrier fragments, liquid and the like with diameters smaller than the standard diameter are removed through the filtering action of the filter 9, and meanwhile, the cleaned cross-linked microcarriers can be conveniently recovered. Further, in order to increase the filtration capacity of the microcarriers, the sealing cap 7 is provided with at least one pair of a feed opening 8 and a filter 9.
The filter 9 in the embodiment of the present application is a funnel-shaped filter, and the filter 9 is detachably connected with the sealing cover 7, as shown in fig. 3. The filter 9 in the embodiment of the present application includes a filter screen 901, a filter membrane stopper 902, and a filter membrane (not shown in the drawings). The top of filter screen 901 is equipped with limiting plate 903 and draw-in groove 904, and this limiting plate 903 can dismantle the setting on sealed lid 7 through rotatory joint's mode, realizes the detachable connection of filter 9 and sealed lid 7. A filter membrane limiting part 902 is arranged in the filter screen 901, and a filter membrane is placed on the filter membrane limiting part 902 and is fixed. The filter screen 901 is clamped in the clamping groove 904 in a rotary clamping mode, so that the fixation of the filter screen 901 and the filter screen limiting piece 902 is realized.
In the embodiment of the application, the pore diameter of the filter membrane is smaller than the standard diameter of the microcarrier, so that the microcarrier and fragments with the diameters smaller than the standard diameter of the microcarrier can be filtered conveniently, and the uniformity of the microcarrier is ensured.
In addition, the stirring device 5 is provided inside the tank 4, and the stirring device 5 is connected to the output end of the motor 3, thereby, when the motor 3 is operated, the stirring device 5 can be driven to stir. Specifically, the stirring device 5 includes a stirring shaft 501 and stirring blades 502 connected, and the stirring shaft 501 is connected to the output end of the motor 3. When the motor 3 is operated, the stirring shaft 501 rotates and drives the stirring blade 502 to rotate, so that the liquid in the tank 4 is stirred to form a vortex flow field. Agitation of the swirling flow field within the tank 4 will cause agitation of the liquid and microcarriers within the filter 9.
To prevent the stirring device 5 from damaging the filter 9 during stirring, the stirring device 5 is located below the filter 9. Since the stirring device 5 is positioned in the tank 4 instead of the filter 9, the microcarriers are not broken by stirring of the stirring device 5, and the quality of the microcarriers is improved.
The bottom of the tank 4 is provided with a liquid outlet pipe 6 for discharging microcarrier chips, liquid and the like. In order to facilitate the discharge of various liquids from the tank 4, a liquid outlet valve 10 is provided at the outlet of the liquid outlet pipe 6.
In addition, the top of sealed lid 7 still is equipped with inlet 11, and inlet 11 intercommunication jar body 4 is inside. The liquid inlet 11 is used for introducing a cross-linking agent liquid or a cleaning liquid into the tank body 4 so as to be convenient for cross-linking with the microcarrier or cleaning the cross-linked microcarrier. The feed inlet 8 and the liquid inlet 11 in the embodiment of the application are both provided with sealing covers, so that pollutants such as dust and the like are prevented from entering the tank body 4 and the filter 9. Meanwhile, in order to facilitate feeding into the tank body 4 and the filter 9, the feed inlet 8 and the liquid inlet 11 are both arranged in a funnel shape.
In an embodiment of the present application, the microcarrier cross-linked cleaning system further comprises a housing 13, which housing 13 is located outside the holder 1 and the tank 4, so as to protect the holder 1 and the tank 4. The sealing cover 7, the feed inlet 8 and the liquid inlet 11 are exposed at the top of the shell 13, so that the tank 4 and the filter 9 can be conveniently fed. The liquid outlet valve 10 on the liquid outlet pipe 6 is exposed out of the shell 13, so that the waste liquid can be conveniently received.
The microcarrier cross-linking cleaning system provided by the embodiment of the application is used for continuously preparing microcarriers, and comprises the steps of microcarrier pretreatment, microcarrier cross-linking, cross-linking microcarrier cleaning and collection, wherein the specific process is as follows:
(1) Microcarrier pretreatment
The sealing cap and the liquid outlet valve 10 at the top of the feed inlet 8 are opened, and the prepared microcarrier suspension is introduced into the filter 9 through the feed inlet 8. In this process, the microcarriers are filtered by the filter 9, leaving microcarriers of standard diameter size inside the filter 9, while microcarriers, microcarrier fragments and liquid smaller than the standard diameter are discharged through the outlet pipe 6.
(2) Microcarrier cross-linking
Closing the liquid outlet valve 10, opening the sealing cover at the top of the liquid inlet 11, and adding the cross-linking agent liquid into the tank body 4. After the cross-linking agent liquid submerges the filter 9, the sealing cover at the top of the liquid inlet is closed. The switch of the motor 3 is turned on to make the motor 3 drive the stirring shaft 501 and the stirring blade 502 to rotate, so that the liquid inside the tank 4 is stirred, and a vortex flow is formed. The swirling flow drives the cross-linking agent and the microcarrier in the filter 9 to rotate, so that the microcarrier is in a moving state, uniform contact between all microcarriers and the cross-linking agent is ensured, and the cross-linking uniformity of the microcarriers is ensured. After the crosslinking is finished, the switch of the motor 3 is closed, the stirring is finished, and the crosslinked microcarrier is settled inside the funnel-shaped filter 9. The outlet valve 10 is opened and the cross-linking agent is discharged through the outlet pipe 6.
(3) Cleaning and collecting the crosslinked microcarrier
Closing the liquid outlet valve 10, opening the sealing cover at the top of the liquid inlet 11, and adding cleaning liquid into the tank body 4. After the cleaning liquid submerges the filter 9, the sealing cover at the top of the liquid inlet 11 is closed. The switch of the motor 3 is turned on to make the motor 3 drive the stirring shaft 501 and the stirring blade 502 to rotate, so that the liquid inside the tank 4 is stirred, and a vortex flow is formed. The vortex flow drives the cleaning solution and the crosslinked microcarrier in the filter 9 to rotate, so that the crosslinked microcarrier is in a moving state, all the crosslinked microcarriers are ensured to be in uniform contact with the cleaning solution, and the residue of the crosslinking agent on the crosslinked microcarrier is reduced. After the cleaning is finished, the switch of the motor 3 is closed, the stirring is finished, and the crosslinked microcarrier is settled inside the funnel-shaped filter 9. The liquid outlet valve 10 is opened and the cleaning liquid is discharged through the liquid outlet pipe 6. The cleaning process can be repeated for a plurality of times until the residual quantity of the cross-linking agent in the cleaning liquid discharged from the liquid outlet pipe 6 meets the requirement.
When the residual quantity of the cross-linking agent in the cleaning liquid discharged from the liquid outlet pipe 6 meets the requirement, the connection between the filter 9 and the sealing cover 7 is opened, the filter 9 is removed, the filter membrane limiting piece 902 is rotated to the clamping groove 904 of the filter screen 901, and the filter membrane is taken out to collect the cross-linked microcarrier deposited on the filter membrane.
The above description is only of the preferred embodiments of the present application and is not intended to limit the present application, but various modifications and variations can be made to the present application by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the protection scope of the present application.
Claims (10)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202422491558.6U CN223197038U (en) | 2024-10-15 | 2024-10-15 | Microcarrier crosslinked cleaning system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202422491558.6U CN223197038U (en) | 2024-10-15 | 2024-10-15 | Microcarrier crosslinked cleaning system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN223197038U true CN223197038U (en) | 2025-08-08 |
Family
ID=96599881
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202422491558.6U Active CN223197038U (en) | 2024-10-15 | 2024-10-15 | Microcarrier crosslinked cleaning system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN223197038U (en) |
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2024
- 2024-10-15 CN CN202422491558.6U patent/CN223197038U/en active Active
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