Background
Centrifuge tubes are a small container commonly used in the laboratory, specifically designed for the experimental operations of sample separation in a centrifuge. As shown in fig. 1, the centrifuge tube in the prior art comprises a tube body 10 and a tube cover 20, wherein the top opening of the tube body 10 forms a tube orifice, the bottom of the tube body 10 is of a conical structure, the tube cover 20 is a pressing type sealing cover, and the tube cover 20 is connected to one side of the tube orifice through a bendable connecting strip. When the centrifuge tube is used, after a sample is added into the centrifuge tube, the tube mouth is closed through the tube cover, the centrifuge tube is placed into the centrifuge tube for centrifugation, so that the sample is layered in the centrifuge tube, then the centrifuge tube is taken out from the centrifuge tube, the tube cover is opened, the suction head of the pipette extends downwards into the centrifuge tube through the tube mouth, and the sample which is correspondingly layered in the centrifuge tube is taken for use.
In some medical detection experimental operations, the centrifuged lower layer sample needs to be taken for use, and the purity of the lower layer sample is important to be kept during sampling. For example, when a patient is subjected to medical tests such as anemia type identification, red blood cell count, and hemoglobin content measurement, whole blood is collected and centrifuged, and the whole blood is centrifuged and is roughly divided into three layers, namely a plasma layer (serum layer), a white blood cell layer, and a red blood cell layer, in this order from top to bottom, and a lower-layer red blood cell sample is taken and used, and the purity of the lower-layer red blood cell sample is maintained during sampling, so that the accuracy of the detection result of red blood cells can be ensured. For example, when biochemical tests, drug concentration tests, virus marker tests, hormone level analyses, electrolyte evaluations, and other medical tests are performed on patients suffering from hyperlipidemia, whole blood is collected and centrifuged, and the upper serum sample is opalescent due to the fact that the upper serum sample contains a large amount of lipid substances, which is called a lipid blood sample, and it is necessary to perform secondary centrifugation on the lipid blood sample, and the lipid blood sample is roughly divided into two layers after secondary centrifugation, namely a chylomide blood lipid layer and a transparent clear serum layer, from top to bottom, and the lower serum sample is taken and used, so that the purity of the lower serum sample is maintained during sampling, and the accuracy of the detection data of serum can be ensured.
The medical detection test operation for taking the lower sample by using the centrifuge tube in the prior art has the following problems that the suction head of the liquid dispenser extends downwards into the centrifuge tube through the tube orifice, the suction head of the liquid dispenser needs to penetrate through the upper sample to suck the lower sample, on one hand, when the suction head of the liquid dispenser penetrates through the upper sample to enter the lower sample, the junction of the upper sample and the lower sample is easy to stir, so that the upper sample is mixed into the lower sample to cause the sucked lower sample to be impure, on the other hand, when the suction head of the liquid dispenser penetrates through the upper sample, part of the upper sample easily enters the suction head of the liquid dispenser or adheres to the surface of the suction head to cause the sucked lower sample to be impure, and therefore, the accuracy of a subsequent detection result is difficult to ensure.
Disclosure of utility model
The utility model aims to provide a simple layered sampling centrifuge tube, so as to solve the technical problem that the sucked lower layer sample is impure when the centrifuge tube in the prior art is used for taking the lower layer sample, thereby influencing the accuracy of a detection result.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
the simple layered sampling centrifuge tube comprises a tube body and a tube cover, wherein the tube cover is detachably and hermetically buckled at the top of the tube body, a bendable connecting strip is connected between the tube body and the tube cover, a sampling structure is arranged at a position, close to the bottom, on the tube body, the sampling structure comprises a sampling hole formed in the tube body and a sampling tube arranged in the tube body, a sealing plug is detachably connected in the sampling hole, one end of the sampling tube is communicated with the sampling hole, and a sealing film is arranged at the other end of the sampling tube.
The principle and beneficial effect of this scheme:
When the sampling tube is in practical application, the tube cover is opened to add a sample into the tube body, and the sealing plug seals the sampling hole, namely, the sealing plug seals one end of the sampling tube, and the sealing film seals the other end of the sampling tube, so that the sample in the tube body cannot enter the sampling tube, and cannot flow out of the tube body through the sampling tube and the sampling hole. Closing the tube cover, putting the centrifuge tube into a centrifuge, and centrifuging the sample to separate the sample into layers in the centrifuge tube. After centrifugation is completed, taking out the centrifuge tube from the centrifuge, taking out the sealing plug from the sampling hole, taking a lower layer sample in the centrifuge tube through the sampling structure by using the pipette, specifically, stretching a suction head of the pipette into the sampling tube through the sampling hole, exerting force on the pipette, enabling the suction head of the pipette to puncture a sealing film, stretching into a position, close to the bottom, in the tube, of the pipette, quantitatively sucking the lower layer sample, taking out the suction head of the pipette from the sampling tube and the sampling hole, sealing the sampling hole again by using the sealing plug, and avoiding outflow of the residual sample in the tube.
1. According to the scheme, the sampling structure is arranged at the position, close to the bottom, on the tube body, after centrifugation is completed, the suction head of the liquid dispenser can directly extend into the position, close to the bottom, in the tube body through the sampling structure, so that the suction head of the liquid dispenser is not required to penetrate through an upper layer sample to directly suck a lower layer sample, the phenomenon that the junction of the upper layer sample and the lower layer sample is muddy when the suction head of the liquid dispenser penetrates through the upper layer sample can be avoided, the upper layer sample is mixed into the lower layer sample, the suction head of the liquid dispenser can be prevented from being firstly introduced into the suction head of the liquid dispenser when the suction head of the liquid dispenser penetrates through the upper layer sample, and the purity of the lower layer sample sucked by the suction head of the liquid dispenser can be guaranteed, and the accuracy of a subsequent detection result is guaranteed.
2. In addition, the sealing plug seals the sampling hole, so that the cleaning in the sampling tube can be kept, and in the sampling operation process after centrifugation is finished, even though the suction head of the liquid transfer device is in contact with the sampling tube, the suction head of the liquid transfer device can be prevented from being polluted, thereby ensuring the purity of the sucked lower-layer sample.
3. The sealing film and the sampling tube of this scheme form the buffer space between sealing plug and inside the body, when carrying out the sampling operation after the sample centrifugation is accomplished, take out the sealing plug from the sample hole and can not lead to the sample of body directly to flow from the sample hole, guarantee going on smoothly of sampling operation, both can avoid the sample extravagant, can avoid sample and experimental environment's two-way pollution again, and when taking out the suction head of pipettor after the sample is accomplished, even if the sample in the body can flow from the position that the sealing film was pricked out, also can flow to the sampling tube at first, will not flow out the body through the sample hole after filling up the sampling tube, reserve the time of sealing the sample hole again with the sealing plug for operating personnel, further prevent that the sample in the body from flowing out.
Preferably, as an improvement, the sealing film has elasticity, and a sampling incision is formed on the sealing film.
The sealing film has the advantages that the sealing film has elasticity, the sampling notch is formed in the sealing film, the sampling notch can be closed under the elastic extrusion action of the sealing film, the tightness of the sealing film is guaranteed, on one hand, the sealing film can be used for double sealing of a sampling structure with a sealing plug, on the other hand, a buffer space can be formed between the sealing plug and the inside of the tube body, a sample is isolated outside the sampling tube, and the sample is prevented from flowing out of the tube body from the sampling hole when the sealing plug is opened.
When the sample centrifugation is finished and then sampling operation is carried out, the suction head of the liquid dispenser stretches into the sampling tube through the sampling hole, the liquid dispenser is forced, the suction head of the liquid dispenser extrudes the sampling notch, and the suction head stretches into the position, close to the bottom, in the tube body through the sampling notch, so that the sampling of the lower-layer sample is completed. And the sampling is accomplished and is withdrawed from the suction head of pipettor, and the sample incision can be reclosed under the elasticity extrusion effect of sealing membrane, continues to isolate the sample outside the sampling tube, avoids lower floor's sample to even in upper strata sample flows to the sampling tube, not only can follow-up secondary sampling, can also seal the sample hole again with the sealing plug at arbitrary time, need not to worry that the sample is full of the sampling tube back and flows out the body through the sample hole, has improved the practicality.
Preferably, as an improvement, the sampling tube is disposed obliquely, and the sealing film is disposed horizontally.
The sample tube is obliquely arranged, the sealing film is horizontally arranged, the centrifugal force in the sample centrifugation process can be avoided, and the sampling notch is prevented from being squeezed out under the action of the centrifugal force, so that the sample is prevented from entering the sample tube through the sampling notch in the centrifugation process, the sample tube, even the sampling hole and the sealing plug are polluted by the sample which is not centrifuged, and the pure lower-layer sample can be smoothly taken through the sampling structure after the centrifugation is ensured.
Preferably, as an improvement, the sampling hole is arranged obliquely, and the axis of the sampling hole coincides with the axis of the sampling tube.
The sampling hole and the sampling tube are coaxially and obliquely arranged, on one hand, the sealing plug can be plugged into the sampling tube through the sampling hole, the contact area of the sealing plug is increased, and therefore the connection stability and the sealing effect of the sealing plug are improved, on the other hand, the included angle between the sampling hole and the sampling tube is eliminated, the diameters of the sampling hole and the sampling tube can be set to be matched with the diameter of the suction head of the liquid transfer device, the volume of the sampling structure is reduced, the suction head of the liquid transfer device can be ensured to extend into the sampling tube smoothly through the sampling hole, and the convenience of sampling operation is improved.
Preferably, as an improvement, the sealing plug comprises an elastic sealing part, and the elastic sealing part is tightly expanded in the sampling hole, so that the sealing plug is detachably connected with the sampling hole.
The sealing plug has the beneficial effects that the sealing plug is arranged to comprise the elastic sealing part, the elastic sealing part is tightly expanded in the sampling hole, the detachable connection of the sealing plug and the sampling hole can be effectively realized, the structure is simple, the operation is convenient, the sealing of the sampling hole can be effectively realized, the sample is prevented from flowing out of the pipe body from the sampling hole, the clean environment in the sampling pipe can be maintained, the purity of the sucked lower-layer sample is ensured, and the accuracy of the subsequent detection result is ensured.
Preferably, as an improvement, the sealing plug further comprises a hard limiting part arranged outside the elastic sealing part, and the size of the hard limiting part is larger than that of the elastic sealing part.
The outer side of the elastic sealing part refers to one side of the elastic sealing part far away from the sampling tube.
The elastic sealing device has the advantages that the hard limiting part is arranged on the outer side of the elastic sealing part, the size of the hard limiting part is larger than that of the elastic sealing part, on one hand, when the elastic sealing part is plugged into the sampling hole to be in tensioning connection, the hard limiting part can limit the elastic sealing part, the elastic limiting part is prevented from being plugged excessively and being difficult to take out, on the other hand, when the elastic limiting part is taken out of the sampling hole, the hard limiting part positioned outside the sampling hole can be used for operating, and the taking-out convenience is improved.
Preferably, as an improvement, the hard limiting part is arc-shaped, and the hard limiting part can be attached to the pipe body.
The centrifugal tube has the beneficial effects that the hard limiting part is arranged into the arc shape, and the hard limiting part is attached to the tube body, so that the volume of the exposed sampling structure outside the tube body can be effectively reduced, the radial volume of the whole centrifugal tube is effectively reduced, and the centrifugal tube can be ensured to be placed in the centrifugal machine for centrifugal treatment.
Preferably, as an improvement, a connecting rope is arranged between the sealing plug and the connecting strip, and the length of the connecting rope is longer than the distance between the sealing plug and the connecting strip.
The connecting rope has the advantages that the sealing plug is connected to the connecting strip through the connecting rope, the situation that the sealing plug is lost after being taken out of the sampling hole is avoided, the sealing plug is used for sealing the sampling hole again later is guaranteed, the length of the connecting rope is larger than the distance between the sealing plug and the connecting strip, namely, when the sealing plug is connected to the sampling hole, the connecting rope has a margin and is in a non-tightening state, an operator can hold the connecting rope, and when the sealing plug is required to be taken out of the sampling hole, the sealing plug can be taken out of the sampling hole through pulling the connecting rope, and the operation convenience of taking out the sealing plug is improved.
Detailed Description
The following is a further detailed description of the embodiments:
Reference numerals in the drawings of the specification comprise a tube body 10, a cylindrical section 11, a conical section 12, a tube cover 20, a connecting strip 21, a sampling structure 30, a sampling hole 31, a sampling tube 32, a sealing plug 40, an elastic sealing part 41, a hard limiting part 42, a sealing film 50, a sampling incision 51 and a connecting rope 60.
Example 1
Simple and easy layering sample centrifuging tube, as shown in figure 2, including body and tube cap 20, tube cap 20 rises tight lock at the body top, realizes the detachable connection of tube cap 20 and body. A bendable connecting strip 21 is connected between the pipe body and the pipe cover 20, one end of the connecting strip 21 is fixed with the pipe body, and the other end of the connecting strip 21 is fixed with the pipe cover 20. The body includes from the top down integrated into one piece's cylindricality section 11 and toper section 12, and the position that is close to the bottom on the body is equipped with sampling structure 30, and the cylindricality section 11 of body is equipped with sampling structure 30 in the position that is close to toper section 12 specifically.
The sampling structure 30 comprises a sampling hole 31 and a sampling tube 32, wherein the sampling hole 31 is obliquely arranged on the cylindrical section 11, the sampling hole 31 penetrates through the cylindrical section 11, the sampling tube 32 is obliquely fixed on the inner wall of the tube body, the top end of the sampling tube 32 is communicated with the sampling hole 31, and the sampling tube 32 and the axis coincide with the axis of the sampling hole 31. In this embodiment, the side wall of the sampling tube 32 is attached to and fixed to the inner wall of the conical section 12 of the tube body, so as to increase the connection area between the sampling tube 32 and the tube body, improve the connection stability between the sampling tube 32 and the tube body, and avoid the sampling tube 32 from falling off in the centrifugation process.
The inside of the sampling hole 31 is detachably connected with a sealing plug 40, the sealing plug 40 comprises an elastic sealing part 41 and a hard limiting part 42, the elastic sealing part 41 is tightly expanded in the sampling hole 31 to realize the detachable connection of the sealing plug 40 and the sampling hole 31, the hard limiting part 42 is fixedly connected to the outer side of the elastic sealing part 41, and the size of the hard limiting part 42 is larger than that of the elastic sealing part 41. The elastic sealing portion 41 is obliquely arranged, and after the elastic sealing portion 41 is plugged into the sampling hole 31 and is tightly fixed, the axis of the elastic sealing portion 41 coincides with the axis of the sampling hole 31. The hard limiting portion 42 is arc-shaped, so that the hard limiting portion 42 can be attached to a tube body, the radial size of the whole centrifuge tube is reduced, and the centrifuge tube can be placed in the centrifuge.
As shown in fig. 3 and 4, the bottom end of the sampling tube 32 is horizontally provided with a sealing film 50, the sealing film 50 is provided with a through sampling incision 51, and the sealing film 50 has elasticity, and the sampling incision 51 is closed under the elastic extrusion action of the sealing film 50. In the present embodiment, the elastic sealing portion 41 and the sealing film 50 of the sealing plug 40 are made of conventional elastic materials such as rubber and silicone, and the present embodiment is not particularly limited as long as the above-described functions can be achieved.
The specific implementation process is as follows:
(1) Sample adding, namely adding a sample to be centrifuged into a space in the tube body, which is positioned outside the sampling tube 32, and tightly fastening the tube cover 20 on the top of the tube body to realize the sealing of the top of the tube body. The elastic sealing part 41 of the sealing plug 40 is in tension connection with the sampling hole 31 to seal the sampling hole 31, thereby sealing the top end of the sampling tube 32. Because the sealing film 50 has elasticity, the sampling notch 51 is closed under the elastic extrusion action of the sealing film 50, so that the bottom end of the sampling tube 32 is sealed. Thus, the sample introduced into the tube body does not enter the sampling tube 32, and does not flow out of the tube body through the sampling tube 32 and the sampling hole 31.
(2) Centrifuging, namely placing the centrifuge tube with the sample into a centrifuge, and centrifuging the sample to separate the sample in the centrifuge tube. Since the sealing film 50 is in a horizontal state, the sampling slit 51 is prevented from being squeezed out by the centrifugal force from the outside horizontally, so that the sample which is not centrifuged is prevented from entering the sampling tube 32 through the sampling slit 51, and the sampling tube 32, even the sampling hole 31 and the sealing plug 40 are prevented from being contaminated.
(3) Sampling, namely taking out the sealing plug 40 from the sampling hole 31 when the lower layer sample is required to be taken out for detection after centrifugation and layering are completed, obliquely extending the suction head of the liquid dispenser into the sampling tube 32 through the sampling hole 31, obliquely downwards exerting force on the liquid dispenser, enabling the suction head of the liquid dispenser to squeeze out the sampling notch 51, and enabling the suction head of the liquid dispenser to extend into the lower portion of the tube body through the sampling notch 51, so that the lower layer sample which is positioned at the lower portion of the tube body after centrifugation can be smoothly sampled. After the sampling is completed, the pipette tip is withdrawn from the sampling tube 32 and the sampling hole 31, the sampling slit 51 is resealed by the elastic pressing of the sealing film 50, and then the sampling hole 31 is resealed using the sealing plug 40.
The lower end of the sampling tube 32 is positioned at the lower part of the tube body, so that the sampling notch 51 on the sealing film 50 is positioned at the lower part of the tube body, after the sample is subjected to centrifugal treatment and layering, the lower layer sample to be taken is also positioned at the lower part of the tube body, and the suction head of the liquid dispenser passes through the sampling notch 51 on the sealing film 50 to directly take the lower layer sample without directly contacting with the upper layer sample, thereby effectively avoiding the pollution of the sucked lower layer sample by the last sample, ensuring the purity of the sucked lower layer sample and further ensuring the accuracy of a detection result.
Example 2
The simple layered sampling centrifuge tube, as shown in fig. 5, is different from the embodiment 1 in that the sampling structure 30 is disposed right under the connecting strip 21, a connecting rope 60 is disposed between the sealing plug 40 and the connecting strip 21, and the length of the connecting rope 60 is longer than the distance between the sealing plug 40 and the connecting strip 21. Specifically, one end of the connecting strip 21 is fastened and fixed on the connecting strip 21, the other end of the connecting strip 21 is adhered and fixed at the middle position of the hard limiting part 42 of the sealing plug 40, and when the sealing plug 40 is connected in the sampling hole 31, the connecting rope 60 has a margin and is in a relaxed state, so that an operator can conveniently hold the connecting rope 60 to pull the sealing plug 40 out of the sampling hole 31, and the sealing plug 40 is detached.
The foregoing is merely exemplary of the present utility model, and specific technical solutions and/or features that are well known in the art have not been described in detail herein. It should be noted that, for those skilled in the art, several variations and modifications can be made without departing from the technical solution of the present utility model, and these should also be regarded as the protection scope of the present utility model, which does not affect the effect of the implementation of the present utility model and the practical applicability of the patent. The protection scope of the present utility model is subject to the content of the claims, and the description of the specific embodiments and the like in the specification can be used for explaining the content of the claims.