CN222274558U - Inoculating device applicable to shaking bottle to bioreactor - Google Patents
Inoculating device applicable to shaking bottle to bioreactor Download PDFInfo
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- CN222274558U CN222274558U CN202420909073.1U CN202420909073U CN222274558U CN 222274558 U CN222274558 U CN 222274558U CN 202420909073 U CN202420909073 U CN 202420909073U CN 222274558 U CN222274558 U CN 222274558U
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Abstract
The utility model discloses an inoculation device suitable for a shake flask transfer bioreactor, which comprises a funnel, an inoculation barrel and a vacuum pump, wherein a small-caliber liquid leakage port of the funnel is communicated with the inoculation barrel through a pipeline, the vacuum pump is communicated with the inoculation barrel through a pipeline, the inoculation barrel is provided with an inoculation barrel liquid discharge pipe, the smooth operation of an inoculation process can be ensured, the smooth culture of a fermentation process is ensured, and a safe and reliable inoculation device is provided for the fermentation production process.
Description
Technical Field
The utility model relates to the technical field of biological inoculation, in particular to an inoculation device applicable to a shake flask transfer bioreactor.
Background
In the fields of bioengineering and biological medicine, large-scale bioreactors are often used for fermentation culture for large-scale production of biological products, wherein the inoculation process of the culture is critical, phage pollution or strain pollution can be caused by improper operation of the inoculation process, the culture cannot be carried out, and the early investment cost is lost.
At present, the inoculation process of fermentation culture can be divided into a primary amplification inoculation process of shaking bottle to large bioreactor and a multistage inoculation process of shaking bottle to small bioreactor and then to large bioreactor according to different amplification stages. In the two inoculation processes, a shaking bottle-to-reactor is needed, a common inoculation method of the shaking bottle-to-reactor is a flame loop method, namely, an alcohol-containing cotton loop is ignited at an inoculation port of the reactor during inoculation, seed liquid is poured into the reactor through the inoculation port on flame, however, the method is difficult and complex to operate, for the process of a plurality of shaking bottle-to-reactor, a plurality of shaking bottles are long in time and difficult to operate in an open mode, pollution risks are difficult to completely avoid, open flame exists in the implementation process of the method, and potential safety hazards are large.
Disclosure of utility model
The utility model aims to provide an inoculating device suitable for a shake flask transfer bioreactor, which is used for solving the problems in the background technology.
In order to achieve the above object, the present utility model provides the following solutions:
An inoculating device suitable for a shake flask transfer bioreactor comprises a funnel, an inoculating barrel and a vacuum pump;
The small-caliber liquid leakage port of the funnel is communicated with the inoculation barrel through a pipeline;
The vacuum pump is communicated with the inoculation barrel through a pipeline;
the inoculation barrel is provided with an inoculation barrel liquid discharge pipe.
Further, a small-caliber liquid leakage port of the funnel is connected with a funnel liquid outlet pipe, and a funnel liquid outlet quick connector is arranged at one end of the funnel liquid outlet pipe away from the funnel;
The inoculating barrel is connected with an inoculating barrel liquid inlet pipe, and one end of the inoculating barrel liquid inlet pipe, which is far away from the inoculating barrel, is provided with an inoculating barrel liquid inlet quick connector;
The funnel liquid outlet quick connector is connected with the inoculation barrel liquid inlet quick connector.
Further, a vacuum pump connecting pipe is connected to the pump interface of the vacuum pump, an inoculation barrel connecting pipe is connected to the inoculation barrel, and the vacuum pump connecting pipe is connected with the inoculation barrel connecting pipe through an air film.
Further, a water level detector is arranged on the funnel liquid outlet pipe, and the water level detector is electrically connected with the vacuum pump through a detection connecting wire.
Further, one end of the inoculation barrel liquid discharge pipe, which is far away from the inoculation barrel, is provided with an inoculation barrel liquid discharge quick connector.
Further, the device also comprises an ultra-clean workbench and a funnel fixing frame arranged in the ultra-clean workbench, wherein the funnel is placed on the funnel fixing frame.
Further, the top of inoculation bucket is provided with the three handhole doors, three connecting hole has been seted up on the three handhole doors, three the connecting hole respectively with inoculation bucket intercommunication, inoculation bucket fluid-discharge tube, inoculation bucket feed liquor pipe and inoculation bucket connecting pipe are respectively through three the connecting hole with inoculation bucket intercommunication.
Compared with the prior art, the utility model has the beneficial effects that:
This device is through increasing an inoculation bucket at inoculation in-process, inoculates the reactor with fungus liquid step by step in, and the in-process that inoculates simultaneously is through the connection of water level detector and vacuum pump in order to increase a feedback system, avoids excessive negative pressure in the inoculation bucket to produce the pollution risk, guarantees going on smoothly of inoculation process, ensures the smooth cultivation of fermentation process, and whole course operates in super clean bench, provides safe and reliable's inoculation device and inoculation mode for fermentation production process, and the device is applicable to the inoculation operation of single reactor and a plurality of reactors simultaneously with the inoculation mode.
Drawings
In order to more clearly illustrate the embodiments of the present utility model or the technical solutions of the prior art, the drawings that are needed in the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present utility model, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of an inoculating device for a shake flask transfer bioreactor according to an embodiment of the present utility model.
Reference numerals illustrate:
1. The device comprises a vacuum pump, a vacuum pump connecting pipe, a detection connecting wire, a 2 inoculation barrel, a 21 three-hole cover, a 22 inoculation barrel connecting pipe, a 23 air film, a 24 inoculation barrel liquid inlet pipe, a 25 inoculation barrel liquid inlet quick connector, a 26 inoculation barrel liquid discharge pipe, a 27 inoculation barrel liquid discharge quick connector, a 3 funnel, a 31 funnel liquid outlet pipe, a 32 funnel liquid outlet quick connector, a 4 water level detector, a 5 funnel fixing frame, a 6 inoculation shaking bottle and a 7 ultra-clean workbench.
Detailed Description
The following describes in further detail the embodiments of the present utility model with reference to the drawings and examples. The following examples are illustrative of the utility model and are not intended to limit the scope of the utility model.
In the description of the present application, it should be understood that the terms "center," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate orientations or positional relationships based on the orientation or positional relationships shown in the drawings, merely to facilitate describing the present application and simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present application.
The terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present application, unless otherwise indicated, the meaning of "a plurality" is two or more.
In the description of the present application, unless explicitly stated or limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected, mechanically connected, electrically connected, directly connected, indirectly connected via an intervening medium, or in communication between two elements. The specific meaning of the above terms in the present application will be understood in specific cases by those of ordinary skill in the art.
The present utility model will be described in further detail with reference to the accompanying drawings for a better understanding of the objects, structures and functions of the present utility model.
Referring to fig. 1, an inoculating device applicable to a shake flask transfer bioreactor of the present embodiment includes a funnel 3, an inoculating barrel 2, a vacuum pump 1, an ultra-clean workbench 7 and an inoculating shake flask 6, wherein a funnel fixing frame 5 is placed in the ultra-clean workbench 7, the funnel 3 is placed on the funnel fixing frame 5 to support the funnel 3, and the ultra-clean workbench 7 ensures sterility of an inoculating process;
The small-caliber liquid leakage port of the funnel 3 is communicated with the inoculation barrel 2 through a pipeline, the vacuum pump 1 is communicated with the inoculation barrel 2 through a pipeline, bacterial liquid in the inoculation shaking bottle 6 is poured into the funnel 3, the vacuum pump 1 is opened to vacuumize the interior of the inoculation barrel 2, a negative pressure environment is formed in the inoculation barrel 2, and bacterial liquid flows into the inoculation barrel 2 along the pipeline;
The inoculation barrel 2 is provided with an inoculation barrel drain pipe 26, and the inoculation barrel 2 can be connected with the reactor through the inoculation barrel drain pipe 26 so as to transfer bacterial liquid in the inoculation barrel 2 into the reactor for inoculation.
Specifically, a small-caliber liquid leakage port of the funnel 3 is connected with a funnel liquid outlet pipe 31, and one end of the funnel liquid outlet pipe 31, which is far away from the funnel 3, is provided with a funnel liquid outlet quick connector 32;
the inoculation barrel 2 is connected with an inoculation barrel liquid inlet pipe 24, and one end of the inoculation barrel liquid inlet pipe 24, which is far away from the inoculation barrel 2, is provided with an inoculation barrel liquid inlet quick connector 25;
The funnel goes out liquid quick-operation joint 32 and is connected with inoculation bucket feed liquor quick-operation joint 25 to connect inoculation bucket 2 and funnel 3 in the mode of quick-operation joint, it is all convenient to install and dismantle.
Specifically, the vacuum pump 1 is connected with a vacuum pump connecting pipe 11 at the pump interface, the inoculation barrel 2 is connected with an inoculation barrel connecting pipe 22, the vacuum pump connecting pipe 11 is connected with the inoculation barrel connecting pipe 22 through an air film 23, the air film 23 can ensure the tightness of the inoculation barrel 2, and the air film 23 is a 0.22 mu m PTFE air film.
Specifically, be provided with water level detector 4 on funnel drain pipe 31, water level detector 4 is through detecting connecting wire 12 and vacuum pump 1 electric connection, is connected with a feedback system in order to increase through water level detector 4 and vacuum pump 1, avoids in the inoculation bucket 2 excessive negative pressure to produce the pollution risk.
Specifically, the inoculating tub drain 26 is provided with an inoculating tub drain quick connector 27 at an end remote from the inoculating tub 2.
Specifically, the top of the inoculation barrel 2 is provided with a three-hole cover 21, three connecting holes are formed in the three-hole cover and are respectively communicated with the inoculation barrel 2, and the inoculation barrel liquid discharge pipe 26, the inoculation barrel liquid inlet pipe 24 and the inoculation barrel connecting pipe 22 are respectively communicated with the inoculation barrel 2 through the three connecting holes.
When bacterial liquid in an inoculation shaking bottle 6 is poured into a funnel 3, a water level detector 4 on a liquid outlet pipe 31 of the funnel detects that the liquid is fed back to a vacuum pump 1, the vacuum pump 1 is started to work, air in an inoculation barrel 2 is pumped out through a vacuum pump connecting pipe 11, negative pressure is formed in the inoculation barrel 2, the bacterial liquid in the funnel 3 can be pressed into the inoculation barrel 2 under the action of atmospheric pressure, so that the operation of transferring the bacterial liquid in the inoculation shaking bottle 6 to the inoculation barrel 2 is completed, after the bacterial liquid completely flows into the inoculation barrel 2, the water level detector 4 detects that no liquid passes through and feeds back to the vacuum pump 1, the vacuum pump 1 stops working, and the bacterial contamination risk caused by excessive negative pressure in the inoculation barrel 2 is prevented;
After the inoculation barrel 2 is transferred, the inoculation barrel 2 can be connected with a reactor through an inoculation barrel liquid discharging quick connector 27, at the moment, gas is filled into the inoculation barrel 2 through a gas film 23, so that positive pressure is formed in the inoculation barrel 2, bacterial liquid enters the reactor through an inoculation barrel liquid discharging pipe 26, and the inoculation process from an inoculation shaking bottle 6 to the reactor is completed;
in addition, for the fermentation process of inoculating a plurality of reactors simultaneously, the inoculating barrel liquid discharging quick connector 27 can be connected with the reactor by disassembling the inoculating barrel 2, and then connected with the quick connector of other reactors, and the inoculating operation of the other reactor can be performed by filling gas into the inoculating barrel 2 and pressing bacteria into the other reactor.
The principles and embodiments of the present utility model have been described herein with reference to specific examples, which are intended to facilitate an understanding of the principles and concepts of the utility model and are to be varied in scope and detail by persons of ordinary skill in the art based on the teachings herein. In summary, the present description should not be construed as limiting the utility model.
Claims (7)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202420909073.1U CN222274558U (en) | 2024-04-28 | 2024-04-28 | Inoculating device applicable to shaking bottle to bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202420909073.1U CN222274558U (en) | 2024-04-28 | 2024-04-28 | Inoculating device applicable to shaking bottle to bioreactor |
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| Publication Number | Publication Date |
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| CN222274558U true CN222274558U (en) | 2024-12-31 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202420909073.1U Active CN222274558U (en) | 2024-04-28 | 2024-04-28 | Inoculating device applicable to shaking bottle to bioreactor |
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| CN (1) | CN222274558U (en) |
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- 2024-04-28 CN CN202420909073.1U patent/CN222274558U/en active Active
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