CN221854610U - Fermentation bacteria inoculation device - Google Patents

Abstract

The utility model relates to a fermentation strain inoculation device, which comprises an inoculation cover, a first inoculation tube, a first valve, an inoculation tank, a second valve, a second inoculation tube and a fermentation tank, wherein the inoculation cover is detachably sealed and connected to one end of the first inoculation tube, the two ends of the inoculation tank are respectively connected to the first inoculation tube and the second inoculation tube, the second inoculation tube is connected to the fermentation tank, the first valve is arranged on the first inoculation tube, and the second valve is arranged on the second inoculation tube. The fermentation strain inoculation device of the utility model can reduce the contamination rate during operation and improve the success rate of aseptic inoculation.

Description

Fermentation bacteria inoculation device

Technical Field

The utility model relates to the technical field of microbial fermentation, in particular to a fermentation bacteria inoculation device.

Background Art

In the process of microbial fermentation production, introducing microbial strains into the seed tank or fermentation tank is the first step of the entire fermentation process. Sterile operation must be ensured. Once contamination occurs during the inoculation process, fermentation failure will occur.

In the existing microbial fermentation production process, flame protection is usually used. After the fermentation tank is reduced to zero pressure, the inoculation cover is opened under the protection of the flame and the seed liquid is poured into the fermentation tank. In this scheme, there is a greater risk of contamination during the process of reducing the pressure to zero. In addition, after the inoculation cover is opened, sterile conditions must be maintained. Usually, the inoculation cover is placed close to the flame, which is cumbersome to operate and the flame may cause damage to the sealing ring of the inoculation cover.

Utility Model Content

Based on the above-mentioned defects in the prior art, the purpose of the present utility model is to provide a fermentation bacteria inoculation device, which can reduce the contamination rate during the operation process and improve the success rate of aseptic inoculation.

To this end, the utility model provides the following technical solutions.

The utility model provides a fermentation bacteria inoculation device, which comprises an inoculation cover, a first inoculation tube, a first valve, an inoculation tank, a second valve, a second inoculation tube and a fermentation tank. The inoculation cover is detachably and sealedly connected to one end of the first inoculation tube, the two ends of the inoculation tank are respectively connected to the first inoculation tube and the second inoculation tube, the second inoculation tube is connected to the fermentation tank, the first valve is arranged on the first inoculation tube, and the second valve is arranged on the second inoculation tube.

Preferably, the fermentation bacteria inoculation device comprises a sterilization tray which is sleeved on the outer circumference of the first inoculation tube, and the sterilization tray is used to support a fire source.

Preferably, the fermentation strain inoculation device comprises a sealing pad, which is installed in the inoculation cover; the fermentation tank is provided with a first air inlet pipe, and the side wall of the inoculation cover is provided with a through hole;

When the inoculation cover is connected to the first position of the first inoculation tube, the sealing gasket seals the port of the first inoculation tube;

When the inoculation cover is connected to the second position of the first inoculation tube, the sealing gasket is separated from the port of the first inoculation tube, the through hole is connected to the first inoculation tube, and the steam introduced into the first air inlet pipe is discharged through the through hole.

Preferably, the fermentation tank is provided with a first air outlet pipe, and the first air inlet pipe cooperates with the first air outlet pipe to perform steam sterilization and/or air pressure regulation on the fermentation tank.

Preferably, the fermentation bacteria inoculation device includes an inoculation tank sterilization assembly, which includes a second air inlet pipe, the second air inlet pipe is provided with a first control valve and one end of the second air inlet pipe is connected to the inoculation tank.

Preferably, the inoculation tank sterilization assembly includes a second air outlet pipe, which is provided with a second control valve; a third control valve is provided at the inlet of the second air inlet pipe, one end of the second air outlet pipe is connected to the second air inlet pipe and the connection point is located between the first control valve and the third control valve.

Preferably, the inoculation tank is a double-conical structure.

Preferably, the inoculation cap is screwed onto the outer circumference of the first inoculation tube.

Preferably, the first valve and the second valve are both non-retention ball valves.

Preferably, the inoculation cover is provided with a handle.

The utility model has the following technical effects:

1. The utility model provides a fermentation strain inoculation device, which provides a temporary storage space for the strain liquid through an inoculation tank. When inoculating the fermentation tank, the inoculation can be performed without reducing the pressure of the fermentation tank, thereby avoiding contamination caused by changes in the pressure in the fermentation tank.

2. The utility model provides a fermentation bacteria inoculation device, which sets a first valve at the inlet of the bacteria liquid of the inoculation tank. When the operation of pouring the bacteria liquid into the inoculation tank is completed, the first valve is closed to block the connection between the first inoculation tube and the inoculation tank, and then the inoculation cover is installed, and then the flame is extinguished. Compared with the existing technical solutions, the sterility requirements for the inoculation cover can be reduced, and the risk of contamination caused by improper sterile operation of the inoculation cover after opening can be avoided. The operation is simpler and safer, and the success rate of sterile inoculation is improved.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG1 is a schematic diagram of a partial structure of a fermentation bacteria inoculation device of the present invention;

FIG2 is an enlarged cross-sectional view of the structure of the fermentation bacteria inoculation device of the present invention when the inoculation cover is connected to the first position of the first inoculation tube;

3 is an enlarged cross-sectional view of the structure of the fermentation bacteria inoculation device of the present invention when the inoculation cover is connected to the second position of the first inoculation tube.

Description of Reference Numerals

100. Fermentation bacteria inoculation device;

1. Inoculation cover; 11. Through hole; 12. Handle;

21. First inoculation tube; 22. Second inoculation tube;

3. The first valve;

4. Inoculation tank;

5. Second valve;

6. fermentation tank; 61. first air inlet pipe; 62. first air outlet pipe;

7. Sterilization tray;

8. Sealing gasket;

9. Sterilization assembly of inoculation tank; 91. Second air inlet pipe; 92. First control valve; 93. Second air outlet pipe; 94. Second control valve; 95. Third control valve.

DETAILED DESCRIPTION

In order to make the technical solution and beneficial effects of the utility model more obvious and easy to understand, the following is a detailed description by listing specific embodiments. Unless otherwise defined, the technical and scientific terms used in this article have the same meaning as those in the technical field to which this application belongs.

In the description of the present invention, unless otherwise explicitly defined, terms such as “center”, “longitudinal”, “lateral”, “length”, “width”, “thickness”, “height”, “up”, “down”, “front”, “back”, “left”, “right”, “vertical”, “horizontal”, “top”, “bottom”, “inside”, “outside”, “clockwise”, “counterclockwise”, etc., indicating directions or positional relationships, are based on the directions or positional relationships shown in the accompanying drawings and are only for the convenience of simplified description of the present invention, and do not indicate that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and should not be understood as a limitation on the present invention.

In the present invention, the terms "first" and "second" are used only for the purpose of clear description and cannot be understood as the relative importance of the indicated features or the number of the indicated technical features. Therefore, the features defined as "first" and "second" can explicitly include at least one of the features. In the description of the present invention, "multiple" means at least two; "several" means at least one, unless otherwise clearly defined.

In the present invention, unless otherwise clearly defined, the terms "install", "connect", "connect", "fix", "set", etc. should be understood in a broad sense. For example, "connection" can be a fixed connection, a detachable connection, or an integral molding; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, or it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

In the present utility model, unless otherwise clearly defined, a first feature being “on”, “above”, “above”, “below”, “below”, “below” or “below” a second feature may mean that the first feature is in direct contact with the second feature, or the first feature and the second feature are in indirect contact through an intermediate medium. Moreover, a first feature being “on”, “above” or “above” a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the horizontal height of the first feature is higher than the horizontal height of the second feature. A first feature being “below”, “below” or “below” a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the horizontal height of the first feature is lower than the horizontal height of the second feature.

The “upper” and “lower” mentioned in the present invention are based on the markings in FIG. 1 .

The fermentation bacteria inoculation device of the present invention will be described in detail below with reference to FIGS. 1 to 3 .

In this embodiment, as shown in Figure 1, the fermentation strain inoculation device 100 includes an inoculation cover 1, a first inoculation tube 21, a first valve 3, an inoculation tank 4, a second valve 5, a second inoculation tube 22 and a fermentation tank 6. The inoculation cover 1 is detachably sealed and connected to one end of the first inoculation tube 21. The two ends of the inoculation tank 4 are respectively connected to the first inoculation tube 21 and the second inoculation tube 22. The second inoculation tube 22 is connected to the fermentation tank 6. The first valve 3 is arranged on the first inoculation tube 21, and the second valve 5 is arranged on the second inoculation tube 22. Before inoculation, the fermentation tank 6 needs to be treated with high-pressure steam for the materials in the tank and the associated pipelines. Therefore, the interior of the fermentation strain inoculation device 100 is sterilized by steam. After sterilization, the inoculation tank 4 is cooled and pressurized by the second valve 5. During inoculation, the first valve 3 remains open and the second valve 5 remains closed. Under the protection of the flame, the inoculation cover 1 is quickly opened to pour the strain liquid from the first inoculation tube 21. When all the strain liquid in the first inoculation tube 21 flows into the inoculation tank 4, the first valve 3 is closed, and then the inoculation cover 1 is installed. The flame is extinguished and the second valve 5 is opened so that the strain liquid in the inoculation tank 4 flows into the fermentation tank 6 through the second inoculation tube 22.

By adopting the above technical solution, the inoculation tank 4 provides a temporary storage space for the bacterial liquid, and when inoculating the fermentation tank 6, the inoculation can be carried out without reducing the pressure of the fermentation tank 6, thereby avoiding bacterial contamination caused by changes in the pressure in the fermentation tank 6. In addition, by setting the first valve 3 at the bacterial liquid inlet of the inoculation tank 4, under the protection of the flame, after the bacterial liquid is poured into the inoculation tank 4, the first valve 3 is first closed to block the connection between the first inoculation tube 21 and the inoculation tank 4, and then the inoculation cover 1 is installed, and then the flame is extinguished. Compared with the existing technical solution, this solution can reduce the sterility requirements for the inoculation cover 1, avoid the risk of bacterial contamination caused by improper aseptic operation of the inoculation cover 1 after opening, and is easier and safer to operate, thereby improving the success rate of aseptic inoculation.

In one embodiment, as shown in FIG. 1 , in order to facilitate the rapid flow of the bacterial liquid, the first inoculation tube 21 , the inoculation tank 4 , the second inoculation tube 22 and the fermentation tank 6 are sequentially distributed from top to bottom.

In one embodiment, as shown in FIG. 1 , the fermentation strain inoculation device 100 includes a sterilization tray 7, which is sleeved on the outer periphery of the first inoculation tube 21, and the sterilization tray 7 is used to support a fire source. After the inoculation cover 1 is opened, the flame ignited on the sterilization tray 7 can provide sterile protection for the pouring of the strain liquid at the entrance of the first inoculation tube 21.

In one embodiment, as shown in Fig. 2 and Fig. 3, the fermentation strain inoculation device 100 includes a sealing gasket 8, which is installed on the top wall of the inner cavity of the inoculation cover 1, the fermentation tank 6 is provided with a first air inlet pipe 61, and the side wall of the inoculation cover 1 is provided with a through hole 11. As shown in Fig. 2, when the inoculation cover 1 is connected to the first position of the first inoculation tube 21, the sealing gasket 8 abuts against the port of the first inoculation tube 21 and seals the cover port. At this time, the through hole 11 is not connected to the first inoculation tube 21, and the outside air cannot enter the fermentation strain inoculation device 100 through the through hole 11, thereby preventing the fermentation strain inoculation device 100 from being contaminated with bacteria. When steam sterilization is required, move the inoculation cover 1 so that the inoculation cover 1 is connected to the second position of the first inoculation tube 21. At this time, the sealing gasket 8 is detached from the port of the first inoculation tube 21, and the through hole 11 is connected to the first inoculation tube 21. The steam introduced by the first air inlet pipe 61 can be discharged through the through hole 11. After passing through the fermentation tank 6, the second inoculation tube 22, the inoculation tank 4 and the first inoculation tube 21 in turn, the steam enters the inoculation cover 1 and is discharged through the through hole 11, so that the interior of the fermentation strain inoculation device 100 can be fully sterilized.

Further, as shown in FIG1 , the fermentation tank 6 is provided with a first air outlet pipe 62, and the first air inlet pipe 61 cooperates with the first air outlet pipe 62 to perform steam sterilization and/or air pressure regulation on the fermentation tank 6. Specifically, when the fermentation tank 6 needs to be steam sterilized separately, steam is introduced from the first air inlet pipe 61, and the steam is discharged from the first air outlet pipe 62 after sterilizing the internal environment of the fermentation tank 6. When the air pressure in the fermentation tank 6 needs to be regulated, the air pressure regulation can be achieved by inletting air from the first air inlet pipe 61 or by exhausting air from the first air outlet pipe 62.

In one embodiment, as shown in FIG1 , the fermentation strain inoculation device 100 includes an inoculation tank sterilization component 9, and the inoculation tank sterilization component 9 includes a second air inlet pipe 91, the second air inlet pipe 91 is provided with a first control valve 92, and one end of the second air inlet pipe 91 is connected to the inoculation tank 4. When the fermentation tank 6 needs to be sterilized again after inoculation in order to repeat the inoculation and/or feeding operation, the second valve 5 is closed, the first control valve 92 is opened, and steam is introduced into the inoculation tank 4 from the second air inlet pipe 91. After the steam flows through the inoculation tank 4 and the first inoculation tube 21 in turn, it enters the inoculation cover 1 and is discharged through the through hole 11, so as to achieve re-sterilization, and the operation is simple. After the sterilization is completed, the second valve 5 is opened, and a small amount of air in the fermentation tank 6 enters the inoculation tank 4, the inoculation tank 4 is cooled and the pressure is maintained, and then the inoculation cover 1 is moved to the first position where it is connected to the first inoculation tube 21.

Further, as shown in FIG1 , the inoculation tank sterilization assembly 9 includes a second air outlet pipe 93, which is provided with a second control valve 94; a third control valve 95 is provided at the entrance of the second air inlet pipe 91, and one end of the second air outlet pipe 93 is connected to the second air inlet pipe 91 and the connection is located between the first control valve 92 and the third control valve 95. When the inoculation tank 4 needs to be sterilized again after the fermentation tank 6 is inoculated, the second valve 5 is closed, the first control valve 92, the second control valve 94 and the third control valve 95 are opened, and part of the steam introduced from the second air inlet pipe 91 enters the inoculation tank 4, and the other part is discharged through the second air outlet pipe 93.

In one embodiment, as shown in FIG. 1 , the inoculation tank 4 is a double-conical structure with a volume of 2-6 L. The inoculation tank 4 is open at both ends and the middle part is bulged. Such a shape structure can increase the storage space of the inoculation tank 4, facilitate the bacterial liquid to flow downward into the fermentation tank 6, and facilitate the steam to flow upward into the first inoculation tube 21. Of course, the structure and shape of the inoculation tank 4 are not limited to this, and can also be a cylinder or a cone.

In one embodiment, as shown in FIG. 2 and FIG. 3 , the inoculation cover 1 is screwed to the outer circumference of the first inoculation tube 21 , and has a simple structure. In addition, the inoculation cover 1 can be opened or installed by rotating, which is easy to operate.

In one embodiment, the first valve 3 and the second valve 5 are both non-retention valves, which avoid residual liquid in the first valve 3 and the second valve 5, thereby preventing the inside of the valve body from rusting and contaminating the bacteria, thereby ensuring the cleanliness of the valve. Preferably, the first valve 3 and the second valve 5 are both ball valves.

In one embodiment, the inoculation cover 1 is provided with a handle 12 to facilitate the user to operate the inoculation cover 1 .

In one embodiment, the first inoculation tube 21 is connected to the inoculation tank 4 by welding, the inoculation tank 4 is connected to the second inoculation tube 22 by welding, and the second inoculation tube 22 is connected to the fermentation tank 6 by welding.

It should be understood that the above embodiments are exemplary and are not intended to include all possible implementations included in the claims. Various modifications and changes may be made on the basis of the above embodiments without departing from the scope of the present disclosure. Similarly, the various technical features of the above embodiments may be arbitrarily combined to form other embodiments of the utility model that may not be explicitly described. Therefore, the above embodiments only express several implementations of the utility model and do not limit the scope of protection of the utility model patent.

Claims (10)

1. Fermentation bacterial inoculation device, its characterized in that, fermentation bacterial inoculation device includes inoculation lid (1), first inoculation pipe (21), first valve (3), inoculation jar (4), second valve (5), second inoculation pipe (22) and fermentation cylinder (6), inoculation lid (1) detachably sealing connection in one end of first inoculation pipe (21), the both ends of inoculation jar (4) respectively with first inoculation pipe (21) with second inoculation pipe (22) intercommunication, second inoculation pipe (22) with fermentation cylinder (6) intercommunication, first valve (3) are located first inoculation pipe (21), second valve (5) are located second inoculation pipe (22).

2. The fermenting species inoculation device according to claim 1, wherein the fermenting species inoculation device comprises a sterilization tray (7) which is sleeved on the periphery of the first inoculation pipe (21), and the sterilization tray (7) is used for supporting a fire source.

3. The fermentation seed inoculating device according to claim 1, comprising a sealing gasket (8) mounted inside the inoculating cap (1); the fermentation tank (6) is provided with a first air inlet pipe (61), and the side wall of the inoculation cover (1) is provided with a through hole (11);

When the inoculating cap (1) is connected to the first position of the first inoculating tube (21), the sealing gasket (8) seals the port of the first inoculating tube (21);

When the inoculation cover (1) is connected to the second position of the first inoculation pipe (21), the sealing gasket (8) is separated from the port of the first inoculation pipe (21), the through hole (11) is communicated with the first inoculation pipe (21), and steam introduced by the first air inlet pipe (61) is discharged through the through hole (11).

4. A fermentation broth inoculation device according to claim 3, wherein the fermenter (6) is provided with a first air outlet duct (62), the first air inlet duct (61) cooperating with the first air outlet duct (62) for steam sterilization and/or air pressure regulation of the fermenter (6).

5. A fermentation broth inoculation apparatus according to claim 3, comprising an inoculation tank sterilization assembly (9) comprising a second air inlet pipe (91), the second air inlet pipe (91) being provided with a first control valve (92) and one end of the second air inlet pipe (91) being connected to the inoculation tank (4).

6. The device according to claim 5, characterized in that the inoculation tank sterilization assembly (9) comprises a second outlet duct (93) provided with a second control valve (94); the inlet of the second air inlet pipe (91) is provided with a third control valve (95), one end of the second air outlet pipe (93) is communicated with the second air inlet pipe (91) and the communication position is located between the first control valve (92) and the third control valve (95).

7. A fermentation broth inoculating device according to any of claims 1-6, wherein the inoculating tank (4) is of biconic configuration.

8. A fermentation broth inoculating device according to any of claims 1-6, wherein the inoculating cap (1) is screw-connected to the outer periphery of the first inoculating tube (21).

9. A fermentation broth inoculating device according to any of claims 1-6, wherein the first valve (3) and the second valve (5) are both detention-free ball valves.

10. A fermentation broth inoculating device according to any of claims 1-6, wherein the inoculating cap (1) is provided with a handle (12).