Microorganism strain breeding device
Technical Field
The utility model relates to the technical field of microbial strain cultivation, in particular to a microbial strain cultivation device.
Background
The microorganism culture method is a method for breeding microorganisms under artificial conditions, according to different types of microorganisms and different requirements on environmental conditions such as nutrients, temperature, oxygen, moisture, pH value and the like, and specific requirements on production and experiments, different culture methods can be adopted, namely an aerobic culture method and an anaerobic culture method, a submerged culture method is suitable for large-scale fermentation culture of aerobic microorganisms, sterile air is introduced into a large-volume liquid culture medium, continuous stirring is carried out, microorganisms can fully contact with the air, and metabolic products can be quickly propagated and accumulated, and the microorganism strain culture device is main equipment for microorganism culture;
The existing partial microorganism strain cultivation device adopts a single-shaft unidirectional stirring mode to mix microorganisms and microorganism culture solution;
There are some problems, such as the adoption of a single-shaft unidirectional stirring mode, which may limit the mixing effect of microorganisms and microorganism culture solution, and further may influence the cultivation effect of microorganism strains.
Disclosure of utility model
The utility model aims to overcome the existing defects, and provides the microbial strain cultivation device which can perform double-layer bidirectional stirring of a microbial culture solution, effectively improve the mixing efficiency of microbial strains and the microbial culture solution, further effectively improve the microbial strain cultivation efficiency and effectively solve the problems in the background technology.
In order to achieve the above purpose, the present utility model provides the following technical solutions: a microorganism strain cultivation device comprises a cultivation tank and a bidirectional driving mechanism;
Cultivation pot: the inside of the cultivation tank is rotationally connected with a rotary drum through a bearing, the inside of the rotary drum is rotationally connected with a rotary column, the upper end of the cultivation tank is provided with a driving bin, the upper end of the rotary column is rotationally connected with the top wall of the driving bin through a bearing II, the lower end of the rotary column is fixedly connected with a connecting column, stirring rods which are uniformly distributed are fixedly connected with the middle parts of the connecting column and the rotary drum, and stirring paddles which are uniformly distributed are fixedly connected with the middle parts of the stirring rods;
Two-way actuating mechanism: the double-layer bidirectional stirring device is arranged in the driving bin, the upper ends of the rotary column and the rotary drum are fixedly connected with the middle part of the bidirectional driving mechanism, so that double-layer bidirectional stirring of the microbial culture fluid can be performed, the mixing efficiency of the microbial strain and the microbial culture fluid is effectively improved, and the microbial strain cultivation efficiency is further effectively improved.
Further, the surface fixedly connected with support of culture tank, the right-hand member fixedly connected with backup pad of support, the rear end fixedly connected with control box of backup pad upper surface, the inside of control box is provided with control switch group, and control switch group's input and external power source electricity are connected, control each electrical apparatus.
Further, two-way actuating mechanism includes drive gear, mount pad, pivot, drive gear and driven gear, drive gear fixedly connected with is in the upper end of column spinner, and the diapire fixedly connected with symmetric distribution's of drive storehouse mount pad, the relative internal surface of mount pad all rotates through the bearing and is connected with the pivot, and the pivot is close to the equal fixedly connected with drive gear of one end of column spinner, and drive gear all is connected with drive gear meshing, and the upper end fixedly connected with driven gear of rotary drum, drive gear all is connected with driven gear meshing, realizes column spinner and rotary drum two-way rotation, and then realizes microorganism and culture solution and sterile air's two-way stirring and mixing.
Further, the bidirectional driving mechanism further comprises a motor, the motor is arranged at the upper end of the driving bin, an output shaft of the motor is fixedly connected with the upper end of the rotary column, and an input end of the motor is electrically connected with an output end of the control switch group to provide driving force for bidirectional stirring and mixing of microorganisms, culture solution and sterile air.
Further, the left end of culture tank upper surface is provided with the inlet pipe, and the middle part of inlet pipe is provided with electric valve one, and the lower extreme of culture tank is provided with the discharging pipe, and the middle part of discharging pipe is provided with electric valve three, and electric valve one and electric valve three's input all are connected with the output electricity of control switch group, realize the business turn over material control of microorganism culture solution and microorganism.
Further, the upper end of backup pad is provided with aseptic air treatment jar, and the upper end outlet duct of aseptic air treatment jar passes through the air inlet intercommunication of connecting pipe and culture tank upper end, and the intake pipe of aseptic air treatment jar communicates with the delivery outlet of outside draught fan, and the front end of culture tank upper surface is provided with the outlet duct, and the middle part of outlet duct is provided with electric valve II, and the input and the output electricity of control switch group of outside draught fan and electric valve II are connected, last provide aseptic air for microorganism bacterial cultivation.
Further, the method. The right-hand member in drive storehouse is articulated through the hinge has the door, and the lower extreme of support is provided with evenly distributed's mounting hole, and the maintenance components and parts of being convenient for effectively ensure microorganism bacterial cultivation job stabilization nature simultaneously.
Compared with the prior art, the utility model has the beneficial effects that: the microbial strain cultivation device has the following advantages:
After the filling is finished, personnel close electric valve one through control switch group, then realize the motor operation through control switch group, the output shaft of motor rotates and drives the column spinner and rotate, the column spinner rotates and drives the spliced pole and rotate, the spliced pole rotates and drives the stirring rod that transversely neighbors and rotate, realize the forward stirring to the culture tank lower floor through the stirring blade that the stirring rod is vertical to be adjacent, the column spinner rotates and drives the drive gear and rotate, and then make two drive gears all rotate under the auxiliary action of the vertical adjacent pivot, the drive gear rotates and makes driven gear rotate, driven gear rotation direction is opposite with the drive gear direction, driven gear reverse rotation drives rotary drum reverse rotation, rotary drum reverse rotation drives the horizontal adjacent stirring rod around the central axis reverse rotation of rotary drum, and then realize the reverse stirring to the culture tank upper strata through vertical adjacent stirring paddle, and then realize the bidirectional stirring mix to microorganism and microorganism culture solution, can carry out the double-deck bidirectional stirring of microorganism culture solution, microorganism strain and culture solution's mixing efficiency with culture solution and sterile air, and then effectively improve microorganism strain cultivation efficiency.
Drawings
FIG. 1 is a schematic diagram of the structure of the present utility model;
FIG. 2 is a schematic cross-sectional view of the interior of the present utility model;
Fig. 3 is an enlarged schematic view of the structure of the present utility model at a.
In the figure: 1 a cultivating tank, 2a bidirectional driving mechanism, 21 a motor, 22 a driving gear, 23 a mounting seat, 24 a rotating shaft, 25 a transmission gear, 26 a driven gear, 3 a rotating column, 4 a rotating drum, 5 a stirring rod, 6 a sterile air treatment tank, 7 an air outlet pipe, 8 a feeding pipe, 9 a first electric valve, 10 a second electric valve, 11 a driving bin, 12 bin gates, 13 a connecting pipe, 14 a supporting plate, 15 a third electric valve, 16 a discharging pipe, 17 a bracket and 18 a control switch group.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Referring to fig. 1-3, the present embodiment provides a technical solution: a microorganism strain cultivation device comprises a cultivation tank 1 and a bidirectional driving mechanism 2;
Cultivation pot 1: the inside of the cultivation pot is rotationally connected with a rotary drum 4 through a bearing, the inside of the rotary drum 4 is rotationally connected with a rotary column 3, the upper end of a cultivation pot 1 is provided with a driving bin 11, the upper end of the rotary column 3 is rotationally connected with the top wall of the driving bin 11 through a bearing II, the lower end of the rotary column 3 is fixedly connected with a connecting column, stirring rods 5 which are uniformly distributed are fixedly connected with the middle parts of the connecting column and the rotary drum 4, stirring paddles which are uniformly distributed are uniformly connected with the middle parts of the stirring rods 5, the outer surface of the cultivation pot 1 is fixedly connected with a bracket 17, the right end of the bracket 17 is fixedly connected with a supporting plate 14, the rear end of the upper surface of the supporting plate 14 is fixedly connected with a control box, the inside of the control box is provided with a control switch group 18, the input end of the control switch group 18 is electrically connected with an external power supply, the right end of the driving bin 11 is hinged with a bin door 12 through a hinge, and the lower end of the bracket 17 is provided with uniformly distributed mounting holes;
Bidirectional drive mechanism 2: the bidirectional driving mechanism is arranged in a driving bin 11, the upper ends of a rotating column 3 and a rotating drum 4 are fixedly connected with the middle part of a bidirectional driving mechanism 2, the bidirectional driving mechanism 2 comprises a driving gear 22, a mounting seat 23, a rotating shaft 24, a transmission gear 25 and a driven gear 26, the driving gear 22 is fixedly connected with the upper end of the rotating column 3, the bottom wall of the driving bin 11 is fixedly connected with symmetrically distributed mounting seats 23, the opposite inner surfaces of the mounting seats 23 are respectively connected with the rotating shaft 24 through three rotation of bearings, one ends of the rotating shafts 24 close to the rotating column 3 are respectively fixedly connected with the transmission gear 25, the transmission gears 25 are respectively connected with the driving gear 22 in a meshing manner, the upper end of the rotating drum 4 is fixedly connected with the driven gear 26, the transmission gears 25 are respectively connected with the driven gear 26 in a meshing manner, the bidirectional driving mechanism 2 further comprises a motor 21, the motor 21 is arranged at the upper end of the driving bin 11, an output shaft of the motor 21 is fixedly connected with the upper end of the rotary column 3, an input end of the motor 21 is electrically connected with an output end of the control switch group 18, the motor 21 runs, the output shaft of the motor 21 rotates to drive the rotary column 3 to rotate, the rotary column 3 rotates to drive the connecting column to rotate, the connecting column rotates to drive the transversely adjacent stirring rod 5 to rotate, forward stirring of the lower layer of the cultivation tank 1 is realized through the stirring blades vertically adjacent to the stirring rod 5, meanwhile, the rotary column 3 rotates to drive the driving gear 22 to rotate, both the two transmission gears 25 rotate under the auxiliary action of the vertically adjacent rotating shafts 24, the transmission gear 24 rotates to drive the driven gear 26 to rotate, the rotation direction of the driven gear 26 is opposite to that of the driving gear 22, the reverse rotation of the driven gear 26 drives the rotary drum 4 to reversely rotate, the reverse rotation of the rotary drum 4 drives the transversely adjacent stirring rod 5 to reversely rotate around the central axis of the rotary drum 4, the reverse stirring of the upper layer of the culture tank 1 is realized through vertically adjacent stirring paddles, and the bidirectional stirring and mixing of microorganisms and microorganism culture solution are further realized;
Wherein: the left end of the upper surface of the cultivation tank 1 is provided with a feed pipe 8, the middle part of the feed pipe 8 is provided with an electric valve I9, the lower end of the cultivation tank 1 is provided with a discharge pipe 16, the middle part of the discharge pipe 16 is provided with an electric valve III 15, and the input ends of the electric valve I9 and the electric valve III 15 are electrically connected with the output end of a control switch group 18;
Wherein: the upper end of backup pad is provided with aseptic air treatment jar, the upper end outlet duct of aseptic air treatment jar passes through the air inlet intercommunication of connecting pipe and culture tank upper end, the intake pipe of aseptic air treatment jar communicates with the delivery outlet of outside draught fan, the front end of culture tank upper surface is provided with the outlet duct, the middle part of outlet duct is provided with electric valve II, the output electricity of outside draught fan and electric valve II and control switch group is connected, the operation of outside draught fan, introduce aseptic air treatment jar 6 with outside air, the inside of aseptic air treatment jar 6 output gets into culture tank 1 through connecting pipe 13, for the cultivation of microorganism bacterial continuously provides aseptic air, electric valve II 10 operates, electric valve II 10 opens, waste gas is discharged to next working area through outlet duct 7.
The working principle of the microorganism strain cultivation device provided by the utility model is as follows: when in work, firstly, the mounting holes evenly distributed at the lower end of the bracket 17 are connected with the threaded holes of the horizontal working area through bolts, so that stable installation of the bracket 17 and the cultivation tank 1 as well as other mechanisms is realized, then, personnel adds microorganism culture solution and microorganisms into the cultivation tank 1 through the feed pipe 8, after filling is finished, the personnel closes the first electric valve 9 through the control switch group 18, then the motor 21 is realized through the control switch group 18, the rotation of the output shaft of the motor 21 drives the rotation column 3 to rotate, the rotation column 3 drives the connection column to rotate, the rotation column drives the transversely adjacent stirring rod 5 to rotate, the vertically adjacent stirring blades of the stirring rod 5 realize forward stirring of the lower layer of the cultivation tank 1, meanwhile, the rotation column 3 drives the driving gear 22 to rotate, and then, the two transmission gears 25 rotate under the auxiliary action of the vertically adjacent rotation shaft 24, the transmission gear 24 rotates to enable the driven gear 26 to rotate, the rotation direction of the driven gear 26 is opposite to that of the driving gear 22, the driven gear 26 reversely rotates to drive the rotary drum 4 to reversely rotate, the rotary drum 4 reversely rotates to drive the transversely adjacent stirring rod 5 to reversely rotate around the central axis of the rotary drum 4, further, reverse stirring of the upper layer of the cultivation tank 1 is achieved through the vertically adjacent stirring paddles, further, bidirectional stirring and mixing of microorganisms and microorganism culture solution are achieved, microorganism strain cultivation efficiency is effectively improved, meanwhile, an external induced draft fan is achieved through the control switch set 18 to operate, external air is introduced into the sterile air treatment tank 6, a filter in the sterile air treatment tank 6 can filter the air entering the sterile tank, microorganisms in the sterile air treatment tank are removed, and in the air filtering process, bacteria in the air can be absorbed by adding coal, fungi and other microorganisms improve the filtering effect, in addition, the sterile air treatment tank 6 is provided with an electric heating tube through self control, a high-temperature environment can be provided, residual microorganisms in the air are killed, the high-temperature sterilization temperature is generally 160-180 ℃, the time lasts for 30-60 minutes, sterile air produced by the sterile air treatment tank 6 enters the interior of the cultivation tank 1 through the connecting tube 13, sterile air is continuously provided for cultivation of microorganism strains, meanwhile, personnel realize operation of the second electric valve 10 through the control switch group 18, the second electric valve 10 is opened, waste gas is discharged to a next working area through the air outlet tube 7, after the cultivation of the microorganism strains is completed, the personnel realize operation of the third electric valve 15 through the control switch group 18, the third electric valve 15 is opened, and the cultivated microorganism strains enter the next working area through the discharging tube 16.
It should be noted that the motor 21 disclosed in the above embodiment may be an ECMA-C20807RS servo driving motor, the first electric valve 9 may be an HK60-D-F electric flange butterfly valve, the second electric valve 10 may be an a20-T8-S2-B electric valve, the third electric valve 15 may be a JG90-D3-DQ-MF7 electric butterfly valve, and the control switch group 18 is provided with control buttons corresponding to the motor 21, the first electric valve 9, the second electric valve 10, the third electric valve 15 and an external induced draft fan one by one and controlling the switch thereof.
The foregoing description is only illustrative of the present utility model and is not intended to limit the scope of the utility model, and all equivalent structures or equivalent processes or direct or indirect application in other related technical fields are included in the scope of the present utility model.