CN221612879U - Reagent disk and biochemical analyzer - Google Patents

Abstract

The present application relates to the technical field of analytical detection equipment, and specifically discloses a reagent tray and a biochemical analyzer, the technical solution of which is as follows: the reagent tray is provided with a circular working area, and at least one sample cup, a plurality of reagent cups and a plurality of reaction cups are provided on the circular working area along its circular track, and the biochemical analyzer includes a reaction tray, a liquid transfer mechanism and an optical signal detection mechanism that can be detachably connected to the reagent tray, and various operations required for biochemical detection are completed by the cooperation of the up and down movement of the liquid transfer head of the liquid transfer mechanism and the rotation of the reaction tray, and the optical signal detection mechanism is used to analyze and detect the optical signal of the reagent or the mixture of the reagent and the sample in the reaction cup. The present application has the effect of rapid detection of multiple items of samples, simple instrument structure, easy operation and accurate results.

Description

Reagent disk and biochemical analyzer

Technical Field

The present application relates to the technical field of analysis and detection equipment, and in particular to a reagent disk and a biochemical analyzer.

Background Art

Biochemical analysis refers to a combination of instruments and reagents that use the principle of photoelectric colorimetry to detect and analyze specific biochemical components in a sample. It can provide important information for the diagnosis, treatment, prevention of diseases and health status assessment in clinical practice, and is an essential basic equipment for medical testing.

In the related technology, clinical biochemical analyzers are currently mostly operated manually or fully automatic. Among them, manual operations such as adding samples, adding reagents, mixing, and testing required for biochemical testing are completed by hand, which is inefficient and has defects such as human errors, which seriously limit its use scenarios. Fully automatic instruments use instruments to automatically complete operations such as adding samples, adding reagents, mixing, testing, cleaning reaction cups, and cleaning sample needles required for biochemical testing. It has the advantages of high detection efficiency and accurate test results. However, since it requires the addition of samples, the addition of reagents, mixing, dilution, cleaning of liquid needles, and cleaning of reaction cups to be designed as corresponding functional modules for implementation, it also has defects such as complex instrument structure, large size, high price, and requires professional personnel to operate, and is not suitable for small sample testing. However, in the vast majority of primary medical institutions (rural health clinics, private clinics, grassroots communities, hospital bedsides, emergency sites, etc.) and animal clinics, due to the fact that most medical staff are non-professional medical testers, the number of test samples is small, the site is small and the funds are limited, there is an urgent need for analytical instruments with simple structure, small size and easy operation to meet the needs of rapid on-site testing.

Especially in some application scenarios, it is necessary to use small equipment to perform rapid biochemical testing of multiple items on samples. Currently, the technology of dry chemical microfluidic biochemical reaction disk is applied to this scenario. This technology can simultaneously complete the detection of more than 20 biochemical items in the sample in more than ten minutes. It has the advantages of small instrument and easy operation. However, this technology has the disadvantages of poor repeatability of test results, complex reagent production process, low yield rate and high cost, which limits its wide application in clinical practice.

Utility Model Content

In order to provide a biochemical analyzer that can perform rapid detection of multiple items on samples at the same time, has a simple instrument structure, is easy to operate, and produces accurate results, the present application provides a reagent disk and a biochemical analyzer.

The present application provides a reagent disk and a biochemical analyzer, which adopt the following technical solutions:

A reagent disc comprises a disc body, wherein the disc body is provided with a circular working area, and at least one sample cup, a plurality of reagent cups and a plurality of reaction cups are arranged on the circular working area along its circular track.

By adopting the above technical solution, the sample cup, reagent cup and reaction cup are integrated in the circular working area of the disk body, so that the sample, reaction cup, first reagent and second reagent elements required for biochemical detection of various projects are integrated on a reagent disk, which can be flexibly controlled and used by rotating the reagent disk. At that time, the pipetting head of the biochemical analyzer only needs to move up and down to achieve the absorption of the corresponding reagent, so that the elements of biochemical detection are integrated, which greatly reduces the dependence on the mechanical structure of the instrument, simplifies the instrument structure, and can make the instrument miniaturized and simple to operate.

Preferably, the tray body is further provided with a sealing member, and the sealing member is used to seal the reagent cup and the reaction cup and is sealedly connected to the reagent cup and the reaction cup.

By adopting the above technical solution, the sealing member seals the reagent cup and the reaction cup, which is convenient for pre-filling the reagents in the reagent cup and the reaction cup, and is beneficial for the storage and transportation of the pre-filled reagents.

Preferably, each of the reaction cups is pre-installed with a first reagent required for all biochemical detection items preset on the reagent disk, the first reagent is a liquid reagent or a solid reagent, and the reaction cup is a transparent material cup.

By adopting the above technical solution, the reaction cup accommodates the first reagent required for each biochemical test respectively, and the reaction cup is a reaction container for each biochemical test item preset on the reagent disk, so that the biochemical test reaction is carried out in the reaction cup. The setting of combining the reagent container and the reaction container into one reduces the volume of the reagent disk. The reaction cup is a transparent material cup to facilitate optical detection of the reagents or the mixture of the reagents and samples of the biochemical test items.

Preferably, each of the reagent cups is pre-installed with a second reagent required for all biochemical test items preset on the reagent disk, and the second reagent is a liquid reagent or a solid reagent.

By adopting the above technical solution, the reagent cup can accommodate each second reagent required for biochemical detection respectively, and when any biochemical reaction requires its specific second reagent to participate in the reaction, the pipetting mechanism can transfer it quantitatively from the reagent cup to the reaction cup.

Preferably, the disk body is further provided with a liquid containing cup along its circular track on the circular working area, the liquid containing cup is pre-filled with cleaning liquid and/or diluent, and the liquid containing cup is sealed by the sealing member.

By adopting the above technical solution, the sealing member seals the liquid-containing cup, which is convenient for pre-filling the cleaning solution and/or diluent in the liquid-containing cup, and is conducive to the storage and transportation of the pre-filled cleaning solution and/or diluent. After the pipette head/pipette tip performs any operation of adding a sample, adding a reagent, and mixing, before switching to the next operation, in order to avoid cross infection, the pipette head/pipette tip can be cleaned in the cleaning solution before performing the next operation of adding a sample, adding a reagent, and mixing. When the sample needs to be diluted, the sample diluent can be pre-filled in the liquid-containing cup, and the sample and the diluent can be quantitatively aspirated and mixed by the pipette head/pipette tip. When the reagents in the reaction cup and the reagent cup are pre-embedded in a solid form, the diluent can be pre-filled in the liquid-containing cup, and the reagent disk can be quantitatively aspirated by the pipette head/pipette tip before the test. The diluent is added to each reaction cup and the reagent cup to re-dissolve the solid reagent before performing biochemical testing.

Preferably, the disk body is further provided with a suction head position along its circular track in the circular working area, and the suction head position is used for placing the suction head.

By adopting the above technical solution, the pipette tip is placed in the pipette tip position, and the pipette tip position is arranged along the circular track of the circular working area, so as to facilitate the pipette tip to be assembled on the pipette head for transferring reagents.

Preferably, the sealing member is any one of an aluminum foil sealing film, a silicone sealing film, a plastic sealing film and a rubber sealing film.

By adopting the above technical scheme, the sealing member is any one of an aluminum foil sealing film, a silicone sealing film, a plastic sealing film and a rubber sealing film, which is conducive to ensuring the sealing. At the same time, the aluminum foil sealing film, the silicone sealing film, the plastic sealing film and the rubber sealing film are economical and practical, and are conducive to manually tearing the reagent disc open or punching it with a punching device before use.

Preferably, the disk body is provided with a connection structure for detachable connection with a biochemical analyzer.

By adopting the above technical solution, the disc body is installed on the reaction disc of the biochemical analyzer through the connecting structure to perform biochemical item detection operations.

Preferably, the reagent disc is integrally formed.

The tray body and various cup shapes are integrally formed by injection molding, which is beneficial to improving the consistency and production efficiency of the reagent tray and making the reagent tray convenient for disposable use.

A biochemical analyzer comprises a reaction disk, a pipetting mechanism and an optical signal detection mechanism. The reaction disk is arranged to rotate horizontally. The reaction disk is provided with a mounting portion for detachably connecting with the disk body of the above-mentioned reagent disk. The optical signal detection mechanism is used to detect optical signals of reagents or mixtures of reagents and samples in reaction cups on the reagent disk. The pipetting mechanism has a pipetting head, and the pipetting head can repeatedly move up and down relative to the reaction disk. Through the cooperation of the up and down movement of the pipetting head and the rotation of the reaction disk, the operations of adding samples, adding reagents and mixing can be realized for the reagents and samples in the reagent disk.

By adopting the above technical scheme, when it is necessary to perform biochemical tests on samples according to the preset biochemical test items on the reagent disk, the staff fixes the disk body of the reagent disk on the reaction disk through the mounting part, and the reaction disk can drive the reagent disk to rotate horizontally, so that the sample cup, reagent cup, reaction cup and liquid cup can all be moved to the position aligned with the pipetting head in the pipetting mechanism. The pipetting mechanism can then realize the transfer of the sample in the sample cup to the reaction cup, the transfer of the reagent in the reagent cup to the reaction cup, the absorption of the diluent from the liquid cup to the reagent cup or the reaction cup to dissolve the solid reagent therein, the cleaning of the pipetting head or the suction tip, and the mixing of the sample and the reagent transferred to the reaction cup, etc. When the mixing operation is completed, the optical signal detection mechanism performs optical signal analysis and detection on the reagent or the mixture of the reagent and the sample, so that the sample can be quickly tested for multiple items, and the instrument structure is simple.

Preferably, a constant temperature heating device is provided in the reaction disk, and the constant temperature heating device heats the reaction disk and heats the reagents in the reagent disk and maintains a predetermined temperature through heat conduction.

By adopting the above technical solution, the constant temperature heating device heats the reaction disk and heats the reagents in the reagent disk through heat conduction and maintains a predetermined temperature, thereby providing a stable temperature environment for biochemical detection and ensuring the stability and reliability of biochemical detection results.

Preferably, the pipetting mechanism includes a pipetting module and a lifting assembly. The pipetting module uses piston-type motion to generate air pressure that is transmitted to a pipetting head through a pipeline. The pipetting head is configured as a small pipeline. The pipetting head inhales and discharges liquid to achieve movement and distribution of the liquid. The pipetting head is fixed on the lifting assembly. The pipetting head is vertically arranged above the reaction disk. As the reaction disk rotates, the lower end of the pipetting head can be aligned with the sample cup, the reagent cup, the reaction cup and the liquid containing cup of the reagent disk respectively. The lifting assembly is arranged on one side of the reaction disk to drive the pipetting head to move vertically up and down.

By adopting the above technical solution, as the reaction disk rotates, the lower end of the pipette head can be aligned with the sample cup, reagent cup, reaction cup and liquid cup respectively, so as to complete operations such as adding samples, adding reagents, and mixing. When the reaction disk rotates to the point where the lower end of the pipette head is aligned with the sample cup, the lifting component drives the pipette head to move vertically up and down to suck out the sample in the sample cup; when the reaction disk rotates to the point where the lower end of the pipette head is aligned with the reagent cup, the lifting component drives the pipette head to move vertically up and down to suck out the reagent in the reagent cup; when the reaction disk rotates to the point where the lower end of the pipette head is aligned with the reaction cup, the lifting component drives the pipette head to move vertically downward, so that the pipette head can be extended into the reaction cup to achieve mixing of the liquid in the reaction cup by repeatedly sucking and releasing the liquid; other operations are performed in the above manner, and examples are not given one by one. In short, through the rotation of the reaction disk, the up and down movement of the pipette head, and the aspiration and discharge actions of the pipette head, the operations required for biochemical testing, such as adding samples to the reaction cup, adding reagents to the reaction cup, mixing the liquid in the reaction cup, cleaning the pipette head/pipette tip in the cleaning solution, adding diluent to the reagent cup, adding diluent to the reaction cup, diluting the sample, etc., can be achieved.

Preferably, the pipetting head can move vertically downward to be detachably and tightly connected with the pipette head placed in the pipette head position.

By adopting the above technical solution, operations such as adding samples, adding reagents, and mixing can be performed by replacing the pipette head with a suction tip, which can greatly reduce the difficulty of cleaning the pipette head, thereby greatly reducing cross contamination of biochemical reactions.

Preferably, it also includes a punching mechanism, which is used to punch holes in the sealing member on the reagent disk, so that the pipette head or suction tip can add samples or perform mixing operations on the sealed reagent cup, the reaction cup and the liquid containing cup without puncture.

By adopting the above technical solution, the punching mechanism punches holes in the seal so that the pipette head can smoothly add samples or mix them in the reagent cup, reaction cup and liquid containing cup. Otherwise, before the test, the sealing layer needs to be torn open manually or the pipette head needs to have a puncture function.

Preferably, the optical signal detection mechanism includes a support seat, a light source, a lens, a filter and an optical signal receiving plate, the support seat is located on one side of the outer periphery of the reaction disk, the light source, the lens and the filter are installed inside the support seat, the optical signal receiving plate is installed inside the reaction disk, and the side wall of the reaction disk is provided with a light-transmitting hole on the light detection path. When the reagent disk is detachably connected to the reaction disk and optical signal detection is performed on the reagent or the mixture of sample and reagent in the reaction cup of the reagent disk, the light source, the lens, the filter, the reaction cup and the optical signal receiving plate are located on the same straight line, and the light emitted by the light source passes through the lens, the filter and the reaction cup in turn and hits the optical signal receiving plate.

By adopting the above technical solution, the support seat supports the light source, the lens and the filter. When the reaction cup needs to be detected, the reaction disk transfers the reaction cup to be detected to the optical path of the optical signal detection mechanism.

Preferably, the optical signal detection mechanism can also be designed based on the post-spectroscopic principle. When this scheme is used to perform optical signal detection on the reaction cup, the light source, the reaction cup, the filter or grating, and the optical signal receiving plate are located on the same straight line. The light emitted by the light source passes through the reaction cup, the filter or grating in turn, and finally shines on the optical signal receiving plate, thereby realizing the detection of the optical signal.

Preferably, the pipetting head of the pipetting mechanism is equipped with an automatic cleaning device, and the automatic cleaning device automatically cleans the pipetting head.

By adopting the above technical solution, the automatic cleaning device automatically cleans the pipette head. Without making major adjustments to the basic structure of the instrument, the liquid cup in the reagent tray for containing the cleaning liquid can be omitted, thereby reducing the complexity of the reagent tray and the volume of the reagent tray, which is conducive to further improving the convenience of the system. When the automatic cleaning technology for the pipette head is adopted, the pipette tip is not used to replace the pipette head for adding samples, adding reagents, and mixing operations.

In summary, the present application includes at least one of the following beneficial technical effects:

1. By integrating the sample cup, reagent cup, reaction cup and liquid cup on a tray, the resource elements required for the detection of multiple biochemical detection items can be integrated on a tray, and the rotation of the tray allows each resource element to be conveniently controlled, thereby greatly reducing the difficulty of providing elements for multiple biochemical detection items, improving the convenience of reagent provision, and greatly reducing the dependence on biochemical detection instruments, simplifying the instrument structure.

2. The biochemical analyzer completes all the actions required for biochemical testing, such as adding samples, adding reagents, mixing, cleaning the pipette head/tip, adding diluent, etc., only by rotating the reaction disk and moving the pipette head of the pipetting mechanism up and down. This greatly simplifies the instrument structure, reduces the instrument cost, reduces the instrument size, makes the instrument easy to operate, and facilitates the product to be used in scenarios requiring rapid on-site testing.

3. The first reagent and the second reagent are set as solid reagents, which are reconstituted with a diluent before use on the reagent disk, which can reduce the temperature requirements for storage and transportation of the reagent disk and further improve the applicability of the reagent disk.

4. By installing a suction tip on the pipetting head to replace the pipetting head for various liquid contact operations, the difficulty of cleaning the pipetting head can be greatly reduced, and the occurrence of cross infection can be reduced. By installing an automatic cleaning device on the pipetting head to clean the pipetting head, the liquid cup in the reagent tray for holding the cleaning liquid can be omitted without making major adjustments to the basic structure of the instrument, thereby reducing the complexity of the reagent tray and the volume of the reagent tray, which is conducive to further improving the convenience of the biochemical analyzer.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG1 is a schematic diagram of the structure of the reagent disk after removing the seal in Example 1 of the present application.

FIG. 2 is a schematic diagram of the structure of the reagent disk when the sealing member is retained in Example 1 of the present application.

FIG3 is a schematic diagram of the structure of the biochemical analyzer in Example 2 of the present application.

FIG. 4 is a schematic structural diagram of the biochemical analyzer in Example 2 of the present application from another perspective.

FIG5 is a cross-sectional view of the biochemical analyzer located at the optical signal detection mechanism in Example 2 of the present application.

FIG. 6 is an enlarged view of portion A in FIG. 5 .

Description of reference numerals:

1. Plate body; 2. Sample cup; 3. Reagent cup; 4. Reaction cup; 5. Seal; 6. Liquid containing cup; 7. Tip position; 8. Tip; 9. Connection structure; 10. Reaction plate; 11. Transfer mechanism; 111. Transfer module; 112. Lifting assembly; 1121. Support column; 1122. Mounting seat; 1123. Driving member; 11231. Driving motor; 11232. Driving screw rod; 1124. Stabilizing block; 113. Transfer head; 12. Optical signal detection mechanism; 121. Support seat; 122. Light source; 123. Lens; 124. Filter; 125. Optical signal receiving board; 13. Limiting block.

DETAILED DESCRIPTION

The present application is further described in detail below in conjunction with Figures 1-6.

Embodiment 1:

Implementation 1 of the present application discloses a reagent disc, referring to FIG1 , comprising a disc body 1, which is arranged in a disc shape, and a circular working area is arranged at the edge of the disc body 1, and at least one sample cup 2, a plurality of reagent cups 3 and a plurality of reaction cups 4 are arranged along the circular track of the circular working area to perform multi-item detection of biochemical analysis.

1 , each reaction cup 4 is pre-installed with the first reagent required for all biochemical detection items preset in the reagent tray. The first reagent is a liquid reagent or a solid reagent. The reaction cup 4 is a transparent cup. In this embodiment, the reaction cup 4 is a colorimetric cup design commonly used in biochemical analyzers to facilitate optical signal detection of the reagent or the mixture of the reagent and the sample in the reaction cup 4.

1 , each reagent cup 3 is pre-installed with a second reagent required for all biochemical test items preset on the reagent disk, and the second reagent is a liquid reagent or a solid reagent.

Referring to FIG1 , the disk body 1 is also provided with a liquid cup 6 along its circular track on the circular working area, and the liquid cup 6 is pre-loaded with a cleaning liquid and/or a diluent. Referring to FIG3 , the cleaning liquid is used to clean the pipette head 113 or the suction tip 8, and the diluent is used to dilute the sample or to redissolve the solid reagents in the reaction cup 4 and the reagent cup 3. Specifically, when the first reagent and the second reagent are set as solid reagents, the diluent in the liquid cup 6 is used to redissolve the solid reagent so that the solid reagent is dissolved into a liquid reagent.

1 and 2, the tray body 1 is also provided with a sealing member 5, which is used to seal the reagent cup 3, the reaction cup 4 and the liquid cup 6 and is sealed and connected with the reagent cup 3, the reaction cup 4 and the liquid cup 6. The sealing member 5 is tightly combined with the openings of the reagent cup 3, the reaction cup 4 and the liquid cup 6 by hot pressing, so as to facilitate the pre-infusion of the reagents in the reagent cup 3 and the reaction cup 4 and the cleaning solution and/or diluent in the liquid cup 6, and is conducive to the storage and transportation of the pre-infused reagents. The sealing member 5 is any one of an aluminum foil sealing film, a silicone sealing film, a plastic sealing film and a rubber sealing film.

Referring to FIG1 , the disk body 1 is also provided with a suction head position 7 along its circular track on the circular working area. Referring to FIG3 , the suction head position 7 is used to place the suction head 8 so as to facilitate the suction head 8 to be assembled on the liquid transfer head 113 for transferring reagents. It should be noted that the suction head 8 is installed on the liquid transfer head 113 to replace the liquid transfer head 113 for liquid suction and discharge operations. Since the suction head 8 is replaceable, the difficulty of cleaning the liquid transfer head 113 can be avoided, and the risk of cross infection can be greatly reduced.

1 , the tray body 1 is provided with a connection structure 9 for detachably connecting with a biochemical analyzer. The tray body 1 is installed on the biochemical analyzer through the connection structure 9 to perform biochemical item detection.

1 , the reagent disc is integrally formed, and the disc body 1 and various cup-shaped ones are integrally injection molded to improve the production efficiency and consistency of the reagent disc, which is conducive to reducing costs and achieving disposable use.

The implementation principle of a reagent disk in Example 1 of the present application is as follows: a sample cup 2, a reagent cup 3, a reaction cup 4 and a liquid containing cup 6 are integrated on a disk body 1, so that elements such as samples, reaction cups 4, first reagents, second reagents, cleaning solutions, etc. required for biochemical tests of various projects are integrated on a reagent disk, and can be flexibly controlled and used by rotating the reagent disk, so that the elements of biochemical tests are concentrated and integrated, which greatly reduces the dependence on the mechanical structure of the instrument, simplifies the structure of the instrument, and can make the instrument miniaturized and simple to operate.

Embodiment 2:

Embodiment 2 of the present application discloses a biochemical analyzer, referring to FIG3 , including a reaction disk 10, a pipetting mechanism 11 and an optical signal detection mechanism 12, wherein the reaction disk 10 is arranged to rotate horizontally, and the reaction disk 10 is provided with a mounting portion for detachably connecting with the disk body 1 of the reagent disk, and the connecting structure 9 of the disk body 1 is inserted into the mounting portion of the reaction disk 10 by a snap-fitting manner to realize the installation of the reagent disk on the reaction disk 10, and at the same time, the rotation of the reaction disk 10 can drive the reagent disk to rotate synchronously. The optical signal detection mechanism 12 is used to perform optical signal detection on the reagent or the mixture of the reagent and the sample in the reaction cup 4 on the reagent disk, and the pipetting mechanism 11 has a pipetting head 113, and the pipetting head 113 can repeatedly move up and down relative to the reaction disk 10, and the reagent and the sample in the reagent disk can be transferred and mixed by the cooperation of the pipetting head 113 and the rotation of the reaction disk 10.

The pipetting mechanism 11 transfers the sample in the sample cup 2 in the reagent disk to the reaction cup 4, transfers the reagent in the reagent cup 3 to the reaction cup 4, draws the diluent from the liquid containing cup 6 to the reagent cup 3 or the reaction cup 4 to re-dissolve the solid reagent therein, cleans the pipetting head 113 or the suction tip 8 of the pipetting mechanism 11, and mixes the sample and reagent transferred to the reaction cup 4, etc. The optical signal detection mechanism 12 is used to analyze and detect the optical signal of the reagent in the reaction cup 4 or the mixture of the reagent and the sample.

3 and 4 , the pipetting mechanism 11 includes a pipetting module 111 and a lifting assembly 112. The pipetting module 111 uses piston motion to generate air pressure which is transmitted to a pipetting head 113 through a pipeline. The pipetting head 113 is configured as a small pipeline. The pipetting head 113 inhales and discharges liquid to achieve quantitative movement and distribution of the liquid. The pipetting head 113 is fixed on the lifting assembly 112. The pipetting head 113 is vertically arranged above the reaction disk 10. As the reaction disk 10 rotates, the lower end of the pipetting head 113 is aligned with the sample cup 2, the reagent cup 3, the reaction cup 4 and the liquid containing cup 6 respectively. The lifting assembly 112 is arranged on one side of the reaction disk 10 to drive the pipetting head 113 to move vertically up and down.

4 , the lifting assembly 112 includes a support column 1121, a mounting seat 1122 and a driving member 1123. The support column 1121 is vertically fixedly arranged on one side of the reaction disk 10. The mounting seat 1122 is slidably connected to the side wall of the support column 1121, and the mounting seat 1122 extends toward the direction of the reaction disk 10. The pipetting head 113 is vertically installed on the side of the mounting seat 1122 facing the reaction disk 10, so that the pipetting head 113 is located above the edge of the reaction disk 10. The driving member 1123 is fixedly installed on the side wall of the support column 1121 and is used to drive the mounting seat 1122 to move vertically up and down. The driving member 1123 drives the mounting seat 1122 to move vertically up and down to drive the pipetting head 113 to move vertically up and down.

4 , the driving member 1123 includes a driving motor 11231 and a driving screw 11232. In the present embodiment, the driving motor 11231 is selected as a servo motor capable of forward and reverse rotation. The driving motor 11231 is fixedly mounted on the side wall of the support column 1121. The output shaft of the driving motor 11231 is vertically arranged upward. The driving screw 11232 is coaxially fixedly connected to the output shaft of the driving motor 11231. The driving screw 11232 is threadedly penetrated through the mounting seat 1122. A side wall of the mounting seat 1122 abuts and slides against the side wall of the support column 1121. The output shaft of the driving motor 11231 rotates to drive the driving screw 11232 to rotate. The driving screw 11232 rotates to drive the mounting seat 1122 to move vertically up and down on the side wall of the support column 1121, thereby realizing the vertical up and down movement of the pipetting head 113 vertically mounted on the mounting seat 1122.

Referring to FIG4 , the lifting assembly 112 further includes a stabilizing block 1124, which is horizontally fixedly disposed on the side wall of the support column 1121. At the same time, a bearing is rotatably disposed in the middle position of the stabilizing block 1124, and the driving screw 11232 is rotatably disposed through the stabilizing block 1124 through the bearing, so as to improve the stability of the driving screw 11232 when rotating. It is worth mentioning that the stabilizing block 1124 is located at the lower end of the driving screw 11232 to limit the vertical downward movement of the mounting seat 1122. Correspondingly, a limiting block 13 is disposed at the upper end of the driving screw 11232, and the limiting block 13 is horizontally fixedly disposed on the side wall of the support column 1121. A bearing is also rotatably disposed in the middle position of the limiting block 13, and the upper end of the driving screw 11232 is rotatably disposed through the limiting block 13 through the bearing, so as to limit the vertical upward movement of the mounting seat 1122.

5 and 6, when it is necessary to perform optical signal detection on the reagent blank, the reagent after adding the sample, and the reaction mixture after adding the second reagent, the reaction disk 10 is rotated to the position where the reaction cup 4 is aligned with the optical path of the optical signal detection mechanism 12 to perform analysis and detection operations. Specifically, the optical signal detection mechanism 12 includes a support seat 121, a light source 122, a lens 123, a filter 124 and an optical signal receiving plate 125. The support seat 121 is fixedly arranged on the upper plate surface of the support plate, and the support seat 121 is located on one side of the outer periphery of the reaction disk 10. The light source 122, the lens 123 and the filter 124 are installed inside the support seat 121, and the light source 122, the lens 123 and the filter 124 are located on the same horizontal plane. The optical signal receiving plate 125 is installed on the reaction disk 1 0, a light-transmitting hole is opened in the side wall of the reaction disk 10 facing the support seat 121. When the reaction cup 4 containing the mixed reagents and samples rotates to a position facing the support seat 121, the light source 122, the lens 123, the filter 124, the reaction cup 4 and the optical signal receiving plate 125 are located on the same straight line. The detection light emitted by the light source 122 passes through the lens 123 and the filter 124 and then enters the mixed reagents and samples in the reaction cup 4, and finally enters the optical signal receiving plate 125 for analysis and detection.

Referring to Fig. 3, a constant temperature heating device is provided in the reaction disk 10, and the constant temperature heating device heats the reaction disk 10 and heats the reagent in the reagent disk and maintains a predetermined temperature through heat conduction. In addition, the biochemical analysis system also includes a punching mechanism and an automatic cleaning device. The punching mechanism is used to punch holes in the sealing member 5, so that the pipetting head 113 can add samples or mix the sealed reagent cup 3, the reaction cup 4 and the liquid containing cup 6 without puncturing. The automatic cleaning device automatically cleans the pipetting head 113. This is a conventional setting in the pipetting device and will not be described in detail here.

The implementation principle of a biochemical analyzer in Example 2 of the present application is as follows: when it is necessary to analyze and test the preset biochemical items on the reagent disk, the biochemical analyzer is first turned on, and the constant temperature heating device in the reaction disk 10 automatically heats and maintains the temperature at 37°C ± 0.2°C, then the reagent disk is installed on the reaction disk 10, the pipette tip 8 is inserted into the pipette tip position 7, the sample to be tested is added to the sample cup 2, the aluminum foil 5 on the reagent disk is manually torn off, and after the detection command is issued to the biochemical analyzer, the reaction disk 10 is rotated to the position where the pipette tip position 7 is directly below the pipette head 113, and the pipette head 113 is driven by the lifting component 112 to move vertically downward to tightly cover the pipette tip 8 on the lower end of the pipette head 113 to complete the automatic installation of the pipette tip 8, and the liquid in the reaction cup 4 and the reagent cup 3 of the reagent disk is filled. After the temperature is raised and kept constant at 37°C±0.2°C, the reaction disk 10 is rotated, the suction head 8 moves up and down, the liquid is sucked and released by the pipetting mechanism 11, the reaction disk 10 rotates and cooperates with the optical signal detection mechanism 12 to detect the optical signal of the reagent or the mixture of the reagent and the sample in the reaction cup 4, and the suction head 8 performs a cleaning operation between two different operation actions. All the biochemical detection items preset on the reagent disk are completed according to their respective detection procedures. The light signal intensity of each analysis stage of all the preset biochemical detection items is obtained, the absorbance value or the absorbance value change of each biochemical detection item is calculated, and compared with the calibration curve of all the biochemical detection items preset on the reaction disk, the detection results of all the biochemical detection items preset on the reagent disk in the sample can be calculated.

The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims (13)

1. A reagent disc, characterized in that: it comprises a disc body (1), the disc body (1) is provided with a circular working area, and at least one sample cup (2), a plurality of reagent cups (3) and a plurality of reaction cups (4) are provided on the circular working area along its circular track; The tray body (1) is further provided with a sealing member (5), and the sealing member (5) is used to seal the reagent cup (3) and the reaction cup (4) and is sealedly connected to the reagent cup (3) and the reaction cup (4); The disk body (1) is also provided with a suction head position (7) along its circular track in the circular working area, and the suction head position (7) is used to place a suction head (8); The disk body (1) is provided with a connection structure (9) for detachably connecting with a biochemical analyzer. 2. A reagent disc according to claim 1, characterized in that each of the reaction cups (4) is pre-installed with the first reagent required for all biochemical detection items preset in the reagent disc, the first reagent is a liquid reagent or a solid reagent, and the reaction cup (4) is a transparent material cup. 3. A reagent disc according to claim 1, characterized in that each of the reagent cups (3) is pre-installed with a second reagent required for all biochemical test items preset in the reagent disc, and the second reagent is a liquid reagent or a solid reagent. 4. A reagent disc according to claim 1, characterized in that: the disc body (1) is also provided with a liquid containing cup (6) along its circular track in the circular working area, the liquid containing cup (6) is pre-filled with a cleaning liquid and/or a diluent, and the liquid containing cup (6) is sealed by the sealing member (5). 5. A reagent disc according to claim 1, characterized in that the sealing member (5) is any one of an aluminum foil sealing film, a silicone sealing film, a plastic sealing film and a rubber sealing film. 6 . The reagent disc according to claim 1 , wherein the reagent disc is integrally formed. 7. A biochemical analyzer, characterized in that it comprises a reaction disk (10), a pipetting mechanism (11) and an optical signal detection mechanism (12), wherein the reaction disk (10) is arranged to rotate horizontally, the reaction disk (10) is provided with a mounting portion for detachably connecting with the disk body (1) of the reagent disk according to any one of claims 1 to 6, the optical signal detection mechanism (12) is used to detect the optical signal of the reagent or the mixture of the reagent and the sample in the reaction cup (4) on the reagent disk, the pipetting mechanism (11) has a pipetting head (113), the pipetting head (113) can repeatedly move up and down relative to the reaction disk (10), and the operation of adding sample, adding reagent and mixing can be realized for the reagent and sample in the reagent disk through the cooperation of the up and down movement of the pipetting head (113) and the rotation of the reaction disk (10); The disk body (1) is also provided with a liquid containing cup (6) along its circular track in the circular working area; The disk body (1) is also provided with a suction head position (7) along its circular track in the circular working area, and the suction head position (7) is used to place a suction head (8). 8. A biochemical analyzer according to claim 7, characterized in that: a constant temperature heating device is arranged in the reaction disk (10), and the constant temperature heating device heats the reaction disk (10) and heats the reagents in the reagent disk through heat conduction and maintains a predetermined temperature. 9. A biochemical analyzer according to claim 7, characterized in that: the pipetting mechanism (11) comprises a pipetting module (111) and a lifting assembly (112), the pipetting module (111) uses piston-type motion to generate air pressure which is transmitted to a pipetting head (113) through a pipeline, the pipetting head (113) is configured as a small pipeline, the pipetting head (113) inhales and discharges liquid to achieve liquid movement and distribution, the pipetting head (113) is fixed on the lifting assembly (112), the pipetting head (113) is located above the reaction disk (10) and is vertically arranged, as the reaction disk (10) rotates, the lower end of the pipetting head (113) can be respectively aligned with the sample cup (2), the reagent cup (3), the reaction cup (4) and the liquid containing cup (6) of the reagent disk, the lifting assembly (112) is arranged on one side of the reaction disk (10) for driving the pipetting head (113) to move vertically up and down. 10. A biochemical analyzer according to claim 7, characterized in that the pipetting head (113) can move vertically downward to be detachably and tightly connected with the pipette head (8) placed in the pipette head position (7). 11. A biochemical analyzer according to claim 7, characterized in that it also includes a punching mechanism, which is used to punch holes in the sealing member (5) on the reagent disk, so that a pipette head (113) or a suction tip (8) can add samples or perform mixing operations on the sealed reagent cup (3), the reaction cup (4) and the liquid containing cup (6) without puncturing. 12. A biochemical analyzer according to claim 7, characterized in that: the optical signal detection mechanism (12) comprises a support seat (121), a light source (122), a lens (123), a filter (124) and an optical signal receiving plate (125), the support seat (121) is located on one side of the periphery of the reaction disk (10), the light source (122), the lens (123) and the filter (124) are installed inside the support seat (121), the optical signal receiving plate (125) is installed inside the reaction disk (10), and the reaction disk (10) A light-transmitting hole is provided on the side wall of the reaction disk (10) on the path of light detection. When the reagent disk is detachably connected to the reaction disk (10) and light signal detection is performed on the reagent or the mixture of the sample and the reagent in the reaction cup (4) of the reagent disk, the light source (122), the lens (123), the filter (124), the reaction cup (4) and the light signal receiving plate (125) are located on the same straight line. The light emitted by the light source (122) passes through the lens (123), the filter (124) and the reaction cup (4) in sequence and then hits the light signal receiving plate (125). 13. A biochemical analyzer according to claim 7, characterized in that the pipetting head (113) of the pipetting mechanism (11) is equipped with an automatic cleaning device, and the automatic cleaning device automatically cleans the pipetting head (113).