CN221544611U

CN221544611U - Stem cell sampler

Info

Publication number: CN221544611U
Application number: CN202323441708.4U
Authority: CN
Inventors: 孙媛媛
Original assignee: Shanghai Junhang Biotechnology Co ltd
Current assignee: Shanghai Junhang Biotechnology Co ltd
Priority date: 2023-12-18
Filing date: 2023-12-18
Publication date: 2024-08-16
Anticipated expiration: 2033-12-18

Abstract

The utility model relates to the technical field of samplers, in particular to a stem cell sampler, which comprises a sampler; a sampling tube which extends downwards and is telescopic is fixed at the center of the connecting cap; the top of the connecting cap is provided with a three-way valve, and one joint of the three-way valve is provided with a drain pipe; when sampling, the connecting cap is sleeved at the bottleneck of the culture bottle, the sampling tube is directly inserted into the bottle bottom of the culture bottle, the piston is pushed to move by the push rod to generate negative pressure in the measuring cylinder, under the action of the negative pressure, stem cell culture fluid in the culture bottle can be sucked into the measuring cylinder through the sampling tube, and when the sample is required to be discharged for detection, the three-way valve is regulated to enable the sampling tube to be communicated with the measuring cylinder, so that the taken stem cell culture fluid can be discharged; the design can control the quantity of the stem cell culture fluid taken out at a time through the measuring cylinder and the like, and can take out more stem cell culture fluid for detection at a time, thereby having the advantages of convenient use and difficult pollution to the culture bottle.

Description

Stem cell sampler

Technical Field

The utility model relates to the technical field of samplers, in particular to a stem cell sampler.

Background

Stem cells are a type of cells capable of self-renewal and differentiating into a plurality of terminally differentiated cells under specific conditions, and various tissues and organs of a human body can be replaced, repaired, rebuilt or regenerated by utilizing a stem cell technology, so that the stem cells have important significance for research and culture of the stem cells; currently, in the course of culturing stem cells, culture can be performed using a flask.

In the process of culturing stem cells in a culture bottle, in order to know the culture state of the stem cells, and the like, part of stem cell culture solution needs to be extracted periodically for detection, and in the sampling process, a sampling tool is needed to be used for taking the stem cell culture solution out of the culture bottle, and a rubber head dropper is usually adopted for sampling the stem cell culture solution, but the rubber head dropper is difficult to control the single sampling amount when sampling, and when extracting more stem cell culture solution, the extraction needs to be repeated for a plurality of times, so that the operation is inconvenient, and pollution of the culture bottle is easy to cause.

Disclosure of utility model

The present utility model aims to solve at least one of the technical problems in the related art to some extent. Therefore, an object of the present utility model is to provide a stem cell sampler, which can control the amount of stem cell culture fluid taken out at a time by a measuring cylinder or the like, and can take out a large amount of stem cell culture fluid for detection at a time, and has the advantages of convenient use and difficult pollution to a culture bottle.

In order to achieve the above purpose, the present utility model provides the following technical solutions:

A stem cell sampler comprising a sampler;

The sampler comprises a connecting cap which can be sleeved at the bottleneck of the culture bottle, and a sampling tube which extends downwards and is telescopic is fixed at the center of the connecting cap;

The sampling tube comprises an upper tube section fixedly connected with the connecting cap, and two limiting rings are fixed on the inner wall of the upper tube section; a spring is arranged between the two limiting rings; a lower pipe section is arranged below the upper pipe section, the top end of the lower pipe section passes through a limiting ring at the bottom, the top end of the lower pipe section is fixedly provided with a slip ring which is in sliding connection with the inner cavity of the upper pipe section, and the bottom end of the outer wall of the lower pipe section is provided with a plurality of sampling ports;

The top of the connecting cap is provided with a three-way valve, and one joint of the three-way valve is provided with a drain pipe;

A measuring cylinder with an opening at the top is fixed at the top of the three-way valve, a connecting pipe is arranged at the top end of the outer wall of the measuring cylinder, and the other two interfaces of the three-way valve are respectively communicated with the measuring cylinder and the sampling pipe; a sealing cover is inserted at the top of the measuring cylinder;

a negative pressure component is arranged above the sealing cover.

In addition, the stem cell sampler proposed according to the application above may further have the following additional technical features:

Specifically, the negative pressure component comprises a tube body which is transversely arranged, a piston is slidably arranged in an opening at the front end of the tube body, a push rod is fixed at the piston, and a push handle is fixed at the tail end of the push rod; the rear end of the pipe body is provided with an exhaust pipe, and the bottom end of the exhaust pipe is fixed with an exhaust pipe which is spliced and matched with the connecting pipe.

Specifically, the measuring cylinder is a transparent finished product made of glass materials, and the outer wall of the measuring cylinder is provided with scales.

Specifically, the top of sealed lid closely bonds there is the connecting seat, and the center department of connecting seat has seted up the fixed orifices that is used for fixed body.

Specifically, the bottom of the lower pipe section is fixed with a convex head which is hemispherical.

Specifically, a valve is arranged on the exhaust pipe.

Specifically, a first one-way valve is arranged at the tail end of the exhaust pipe, and a second one-way valve is arranged in the exhaust pipe.

Specifically, the size of the piston is matched with the size of the front end opening of the pipe body, and the piston is a finished product made of rubber materials.

Compared with the prior art, the utility model has the beneficial effects that:

1. Through the sampler that sets up: when sampling, the connecting cap is sleeved at the bottleneck of the culture bottle, the sampling tube is directly inserted into the bottle bottom of the culture bottle, the piston is pushed to move by the push rod to generate negative pressure in the measuring cylinder, under the action of the negative pressure, stem cell culture fluid in the culture bottle can be sucked into the measuring cylinder through the sampling tube, and when the sample is required to be discharged for detection, the three-way valve is regulated to enable the sampling tube to be communicated with the measuring cylinder, so that the taken stem cell culture fluid can be discharged; the design can control the quantity of the stem cell culture fluid taken out at a time through the measuring cylinder and the like, and can take out more stem cell culture fluid for detection at a time, thereby having the advantages of convenient use and difficult pollution to the culture bottle;

2. through the telescopic sampling tube that sets up, do benefit to the sampling tube and insert in the blake bottle to different degree of depth and take a sample, improved the convenience of sampling operation.

Drawings

FIG. 1 is a schematic diagram of the overall structure of the present utility model;

FIG. 2 is a schematic view of the present utility model in use;

FIG. 3 is a cross-sectional view of the overall structure of the present utility model;

FIG. 4 is an enlarged schematic view of the portion A of FIG. 3 according to the present utility model;

FIG. 5 is a cross-sectional view of the structure of a sampling tube according to the present utility model;

Fig. 6 is a schematic view of a part of an exploded structure in the present utility model.

In the figure:

1. A sampler; 10. a connecting cap; 11. a sampling tube; 111. an upper pipe section; 112. a limiting ring; 113. a spring; 114. a lower pipe section; 1141. a sampling port; 115. a slip ring; 116. a nose; 12. a three-way valve; 13. a discharge tube; 14. a measuring cylinder; 141. a connecting pipe; 15. sealing cover; 151. a connecting seat; 16. a negative pressure assembly; 161. a tube body; 162. a piston; 163. a push rod; 164. a push handle; 165. an exhaust pipe; 1651. a first one-way valve; 166. a valve; 167. an exhaust pipe; 1671. and a second one-way valve.

Description of the embodiments

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

The embodiment provides a technical scheme:

Referring to fig. 1-6, a stem cell sampler includes a sampler 1. The sampler 1 comprises a connecting cap 10 sleeved at the bottleneck of the culture flask, and a sampling tube 11 which extends downwards and is telescopic is fixed at the center of the connecting cap 10; the sampling tube 11 comprises an upper tube section 111 fixedly connected with the connecting cap 10, and two limiting rings 112 are fixed on the inner wall of the upper tube section 111; a spring 113 is arranged between the two limiting rings 112; a lower pipe section 114 is arranged below the upper pipe section 111, the top end of the lower pipe section 114 passes through a limiting ring 112 at the bottom, a slip ring 115 which is in sliding connection with the inner cavity of the upper pipe section 111 is fixed at the top end of the lower pipe section 114, and a plurality of sampling ports 1141 are arranged at the bottom end of the outer wall of the lower pipe section 114. The top of the connecting cap 10 is provided with a three-way valve 12, and one interface of the three-way valve 12 is provided with a drain pipe 13; a measuring cylinder 14 with an opening at the top is fixed at the top of the three-way valve 12, a connecting pipe 141 is arranged at the top end of the outer wall of the measuring cylinder 14, and the other two interfaces of the three-way valve 12 are respectively communicated with the measuring cylinder 14 and the sampling pipe 11; a sealing cover 15 is inserted at the top of the measuring cylinder 14; a negative pressure assembly 16 is arranged above the sealing cover 15. The negative pressure assembly 16 comprises a tube body 161 which is transversely arranged, a piston 162 is slidably arranged in an opening at the front end of the tube body 161, a push rod 163 is fixed at the piston 162, and a push handle 164 is fixed at the tail end of the push rod 163; an exhaust pipe 165 is installed at the rear end of the pipe body 161, and an exhaust pipe 167 which is in plug-in fit with the connecting pipe 141 is fixed at the bottom end of the exhaust pipe 165.

In this embodiment, the measuring cylinder 14 is a transparent glass product, and the outer wall of the measuring cylinder 14 is provided with graduations. The transparent design and the graduation design facilitate the observation of the amount of stem cell culture fluid withdrawn from the graduated cylinder 14.

In this embodiment, the top of the sealing cover 15 is tightly adhered with a connection seat 151, and a fixing hole for fixing the pipe body 161 is formed in the center of the connection seat 151. The connecting seat 151 is tightly adhered to the pipe body 161, and the negative pressure assembly 16 is supported and fixed by the arrangement of the connecting seat 151.

In this embodiment, the bottom of the lower pipe section 114 is fixed with a protruding head 116, and the protruding head 116 is hemispherical. When the raised head 116 abuts against the bottom of the culture flask, the raised head 116 is hemispherical, so that damage caused by excessive extrusion of the raised head 116 on the bottom of the culture flask can be avoided.

In this embodiment, valve 166 is mounted on exhaust pipe 165. The venting of exhaust tube 165 may be controlled by the provision of valve 166, facilitating control of the amount of sampling by controlling the bleed.

In this embodiment, a first check valve 1651 is mounted at the end of exhaust pipe 165, and a second check valve 1671 is mounted in exhaust pipe 167. Air in exhaust pipe 165 cannot enter into connecting pipe 141 through second check valve 1671, air in connecting pipe 141 can flow back into exhaust pipe 165 through second check valve 1671, air from outside cannot enter into exhaust pipe 165 and pipe body 161 through first check valve 1651, and air in exhaust pipe 165 and pipe body 161 can be discharged to outside through first check valve 1651; this design facilitates the creation of negative pressure within cylinder 14 by movement of piston 162.

In this embodiment, the size of the piston 162 is adapted to the size of the front opening of the tube 161, and the piston 162 is made of rubber. This design facilitates smooth sliding of piston 162 within tube 161 while facilitating the creation of negative pressure by movement of piston 162.

Before specific use, a user firstly inserts the sampling tube 11 into the bottle mouth of the culture bottle in alignment with the bottle mouth of the culture bottle, the upper tube section 111 and the lower tube section 114 are inserted into the culture bottle, the raised head 116 abuts against the bottom of the culture bottle, the sliding ring 115 slides along the inner cavity of the upper tube section 111, the spring 113 is compressed at the same time, and then the user sleeves the connecting cap 10 at the bottle mouth of the culture bottle;

when the device is specifically used, a user holds the push rod 163 and pulls the push rod, at this time, air in the measuring cylinder 14 enters the pipe body 161 through the connecting pipe 141, the exhaust pipe 167 and the exhaust pipe 165, negative pressure is generated in the measuring cylinder 14, stem cell culture solution in the culture bottle flows into the measuring cylinder 14 through the lower pipe section 114, the upper pipe section 111 and the three-way valve 12 under the action of the negative pressure, when the stem cell culture solution in the measuring cylinder 14 reaches a preset scale, the user tightens the valve 166 and stops pulling the push rod 163, then the user adjusts the three-way valve 12 to enable the discharge pipe 13 to be communicated with the measuring cylinder 14, and the stem cell culture solution in the measuring cylinder 14 flows into the container through the discharge pipe 13 by using the container, so that sampling is completed.

The foregoing has shown and described the basic principles, principal features and advantages of the utility model. It will be understood by those skilled in the art that the present utility model is not limited to the above-described embodiments, and that the above-described embodiments and descriptions are only preferred embodiments of the present utility model, and are not intended to limit the utility model, and that various changes and modifications may be made therein without departing from the spirit and scope of the utility model as claimed. The scope of the utility model is defined by the appended claims and equivalents thereof.

Claims

1. Stem cell sampler, including sampler (1), its characterized in that:

The sampler (1) comprises a connecting cap (10) sleeved at the bottleneck of the culture flask, and a sampling tube (11) which extends downwards and is telescopic is fixed at the center of the connecting cap (10);

The sampling tube (11) comprises an upper tube section (111) fixedly connected with the connecting cap (10), and two limiting rings (112) are fixed on the inner wall of the upper tube section (111); a spring (113) is arranged between the two limiting rings (112); a lower pipe section (114) is arranged below the upper pipe section (111), the top end of the lower pipe section (114) passes through a limiting ring (112) at the bottom, a slip ring (115) which is in sliding connection with the inner cavity of the upper pipe section (111) is fixed at the top end of the lower pipe section (114), and a plurality of sampling ports (1141) are formed at the bottom end of the outer wall of the lower pipe section (114);

The top of the connecting cap (10) is provided with a three-way valve (12), and one interface of the three-way valve (12) is provided with a drain pipe (13);

A measuring cylinder (14) with an opening at the top is fixed at the top of the three-way valve (12), a connecting pipe (141) is arranged at the top end of the outer wall of the measuring cylinder (14), and the other two interfaces of the three-way valve (12) are respectively communicated with the measuring cylinder (14) and the sampling pipe (11); a sealing cover (15) is inserted at the top of the measuring cylinder (14);

a negative pressure component (16) is arranged above the sealing cover (15).

2. The stem cell sampler of claim 1 wherein: the negative pressure assembly (16) comprises a tube body (161) which is transversely arranged, a piston (162) is slidably arranged in an opening at the front end of the tube body (161), a push rod (163) is fixed at the position of the piston (162), and a push handle (164) is fixed at the tail end of the push rod (163); an exhaust pipe (165) is arranged at the rear end of the pipe body (161), and an exhaust pipe (167) which is in plug-in connection with the connecting pipe (141) is fixed at the bottom end of the exhaust pipe (165).

3. The stem cell sampler of claim 1 wherein: the measuring cylinder (14) is a transparent glass manufactured product, and the outer wall of the measuring cylinder (14) is provided with scales.

4. The stem cell sampler of claim 1 wherein: the top of the sealing cover (15) is tightly adhered with a connecting seat (151), and a fixing hole for fixing the pipe body (161) is formed in the center of the connecting seat (151).

5. The stem cell sampler of claim 1 wherein: the bottom of the lower pipe section (114) is fixed with a protruding head (116), and the protruding head (116) is hemispherical.

6. The stem cell sampler of claim 2, wherein: a valve (166) is mounted on the exhaust pipe (165).

7. The stem cell sampler of claim 2, wherein: a first one-way valve (1651) is arranged at the tail end of the exhaust pipe (165), and a second one-way valve (1671) is arranged in the exhaust pipe (167).

8. The stem cell sampler of claim 2, wherein: the size of the piston (162) is matched with the size of the front end opening of the pipe body (161), and the piston (162) is made of rubber.