CN221371174U - Multifunctional seed culture and transplanting device for aseptic fermentation - Google Patents
Multifunctional seed culture and transplanting device for aseptic fermentation Download PDFInfo
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- CN221371174U CN221371174U CN202323211199.6U CN202323211199U CN221371174U CN 221371174 U CN221371174 U CN 221371174U CN 202323211199 U CN202323211199 U CN 202323211199U CN 221371174 U CN221371174 U CN 221371174U
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- 238000000855 fermentation Methods 0.000 title claims abstract description 29
- 230000004151 fermentation Effects 0.000 title claims abstract description 29
- 238000011218 seed culture Methods 0.000 title claims abstract description 28
- 238000011081 inoculation Methods 0.000 claims abstract description 58
- 238000004140 cleaning Methods 0.000 claims abstract description 50
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 47
- 239000000463 material Substances 0.000 claims abstract description 44
- 230000001954 sterilising effect Effects 0.000 claims abstract description 43
- 238000005070 sampling Methods 0.000 claims abstract description 36
- 230000000007 visual effect Effects 0.000 claims description 8
- 238000004321 preservation Methods 0.000 claims description 3
- 230000008447 perception Effects 0.000 claims 2
- 230000000813 microbial effect Effects 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 description 11
- 239000001963 growth medium Substances 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 238000000034 method Methods 0.000 description 6
- 229910000831 Steel Inorganic materials 0.000 description 5
- 238000012258 culturing Methods 0.000 description 5
- 239000010959 steel Substances 0.000 description 5
- 238000010276 construction Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000007599 discharging Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000011540 sensing material Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
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Abstract
The utility model relates to the technical field of microbial seed fermentation, in particular to a multifunctional seed culture and transplanting device for aseptic fermentation. The device comprises a tank body, wherein a charging port, a pressure sensor for sensing the pressure in the tank body and a thermometer for sensing the temperature of materials in the tank body are arranged at the upper end of the tank body; the air inlet and seed transfer structure comprises an air inlet and seed transfer pipe which is inserted into the tank body from the top end of the tank body to the bottom of the tank body, and the air inlet and seed transfer pipe exposed outside the tank body is sequentially connected with an air inlet structure, a steam sterilization structure, an inoculation sterilization structure and an inoculation structure; the air inlet structure is reversely connected with a sampling structure. The seed culture and transplanting device integrates the functions of tank body cleaning, thallus seed culture, thallus disinfection and transplanting, and has the advantages of convenient operation and high thallus culture efficiency.
Description
Technical Field
The utility model relates to the technical field of microbial seed fermentation, in particular to a multifunctional seed culture and transplanting device for aseptic fermentation.
Background
The prior fermentation generally comprises multi-stage seed liquid, including inclined seed liquid, primary seed liquid, secondary seed liquid and the like, wherein the primary seed liquid is subjected to shaking culture under proper conditions by utilizing a plurality of shaking bottles, then the primary seed liquid of each bottle is combined into a large steel bottle, and then the mixed primary seed liquid is transferred to a secondary seed tank, so that the whole process is required to be completed under a sterile state. Chinese patent CN201720691054.6 discloses a seed transfer bottle, which is a device that combines cultured primary seed solutions, and then transfers the seed to a secondary seed tank. The prior art has the following problems: 1. the volume of the first-stage seed liquid usually reaches several liters, more shake flasks are needed for culturing, each shake flask needs repeated batching, sterilization, inoculation and culture, and then the operations of merging into a steel bottle are carried out, so that the steps are numerous, the operation is complex, and the contaminated bacteria are easy to cause. 2. Shake flasks are typically sterilized off-line using a sterilization kettle, and require special shaking tables and sterile rooms for culture, requiring stringent facilities and sites. 3. The primary seed liquid of the shaking bottle is generally required to be combined into a slightly large steel bottle and then transferred to a secondary seed tank, and as the primary seed liquid is required to be combined into the large steel bottle after being cultured, the quantity of the shaking bottles is generally large, and the risk of once contaminating infectious microbe is increased in the process of pouring the seed liquid into the steel bottle each time.
Disclosure of Invention
Aiming at the technical defects, the utility model solves the problems that the prior art is easy to cause pollution to strains and strict in requirements on facilities and sites, and provides a multifunctional seed culture and transplanting device for aseptic fermentation.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows: the multifunctional seed culturing and transplanting device for aseptic fermentation comprises a tank body, wherein a feed inlet, a pressure sensor for sensing the pressure in the tank body and a thermometer for sensing the temperature of materials in the tank body are arranged at the upper end of the tank body, and the upper end of the tank body is connected with an exhaust structure, a cleaning structure, a material pressing structure, an air inlet and transplanting structure; the air inlet and seed transfer structure comprises an air inlet and seed transfer pipe which is inserted into the tank body from the top end of the tank body to the bottom of the tank body, and the air inlet and seed transfer pipe exposed outside the tank body is sequentially connected with an air inlet structure, a steam sterilization structure, an inoculation sterilization structure and an inoculation structure; the air inlet structure is reversely connected with a sampling structure.
Further: in the multifunctional seed culture and transplanting device for aseptic fermentation, the exhaust structure comprises an exhaust pipe communicated with the inside of the tank body and an exhaust valve arranged on the exhaust pipe.
The cleaning structure comprises a cleaning pipe communicated with the inside of the tank body and a cleaning valve arranged on the cleaning pipe, and a cleaning ball is arranged at the top end of the cleaning pipe in the tank body.
The material pressing structure comprises a material pressing air pipe communicated with the inside of the tank body and a material pressing valve arranged on the material pressing air pipe.
The air inlet structure comprises an air inlet pipe communicated with the air inlet pipe and the seed transfer pipe and an air inlet valve arranged on the air inlet pipe; the sampling structure is reversely communicated with the air inlet pipe and comprises a sampling pipe reversely communicated with the air inlet pipe, and a sampling valve is arranged on the sampling pipe; the air inlet pipe and the material pressing air pipe are connected in parallel and then connected with an air pipe, and an air valve and an air filter are sequentially arranged on the air pipe. The upper end of the air filter is provided with a pressure gauge, and the lower end of the air filter is provided with a filter blow-down valve.
The steam sterilizing structure comprises a steam pipe communicated with the air inlet and the seed transferring pipe and a steam valve arranged on the steam pipe, a steam sterilizing valve is arranged between the tail end of the steam pipe and the tail end of the air pipe, and the front end of the steam sterilizing structure is provided with the seed transferring valve.
The inoculation and disinfection structure comprises an inoculation and disinfection steam pipe communicated with the air inlet pipe and the seed transfer pipe, an inoculation and disinfection valve is arranged on the inoculation and disinfection steam pipe, and a clamp flange is arranged at the front end of the inoculation and disinfection structure.
The inoculation structure comprises an inoculation pipe communicated with the air inlet and the seed transfer pipe and an inoculation valve arranged at the front end of the inoculation pipe.
Still further: in the multifunctional seed culture and transplanting device for aseptic fermentation, a visual window is arranged on the tank body; the outer side wall of the tank body is provided with a heat-insulating jacket; the bottom of the tank body is provided with a hydraulic lifting device for vertically lifting the tank body.
The lower end of the whole cultivating and seed transplanting device is provided with a universal wheel which is convenient for the cultivating and seed transplanting device to horizontally move, and the universal wheel is provided with a brake structure.
Compared with the prior art, the multifunctional seed culture and seed transfer device for aseptic fermentation comprises a tank body, wherein a feed inlet, a pressure sensor for sensing the pressure in the tank body and a thermometer for sensing the temperature of materials in the tank body are arranged at the upper end of the tank body, and the upper end of the tank body is connected with an exhaust structure, a cleaning structure, a material pressing structure, an air inlet and a seed transfer structure; the air inlet and seed transfer structure comprises an air inlet and seed transfer pipe which is inserted into the tank body from the top end of the tank body to the bottom of the tank body, and the air inlet and seed transfer pipe exposed outside the tank body is sequentially connected with an air inlet structure, a steam sterilization structure, an inoculation sterilization structure and an inoculation structure; the air inlet structure is reversely connected with a sampling structure. The seed culture and transplanting device integrates the functions of tank body cleaning, thallus seed culture, thallus disinfection and transplanting, and has the advantages of convenient operation and high thallus culture efficiency.
Drawings
FIG. 1 is a schematic diagram showing the structure of a multifunctional seed culture and transplanting device for aseptic fermentation according to an embodiment;
FIG. 2 is a schematic diagram showing the construction of a multi-functional seed culture and transplanting apparatus for aseptic fermentation according to the second embodiment;
FIG. 3 is a schematic view showing the construction of a multi-functional seed culture and transplanting apparatus for aseptic fermentation according to the third embodiment;
Wherein: 1 tank body, 2 charging port, 3 pressure sensor, 4 air inlet and seed transfer pipe, 5 exhaust pipe, 6 exhaust valve, 7 cleaning pipe, 8 cleaning valve, 9 cleaning ball, 10 air compressing pipe, 11 air compressing valve, 12 air inlet pipe, 13 air inlet valve, 14 sampling pipe, 15 sampling valve, 16 air pipe, 17 air valve, 18 air filter, 19 steam pipe, 20 steam valve, 21 steam sterilizing valve, 22 seed transfer valve, 23 inoculation sterilizing steam pipe, 24 inoculation sterilizing valve, 25 clamp flange, 26 inoculation pipe, 27 inoculation valve, 28 visual window, 29 hydraulic lifting device, 30 universal wheel, 31 filter blow-down valve, 32 heat preservation jacket, 33 thermometer.
Detailed Description
The multifunctional seed culture and seed transfer device integrates the functions of tank body cleaning, thallus seed culture, thallus disinfection, seed transfer and the like, and has the advantages of convenient operation and high thallus culture efficiency. The present utility model will be further described in detail with reference to the following examples, which are not intended to limit the scope of the utility model, but rather the choice of the individual raw materials in the material will not have any substantial effect on the results depending on the particular circumstances.
Embodiment one: as shown in figure 1, the multifunctional seed culture and seed transfer device for aseptic fermentation comprises a tank body 1, wherein a feed inlet 2, a pressure sensor 3 for sensing the pressure in the tank body and a thermometer 33 for sensing the temperature of materials in the tank body are arranged at the upper end of the tank body, and an exhaust structure, a cleaning structure, a material pressing structure, an air inlet and seed transfer structure are connected at the upper end of the tank body; the air inlet and seed transfer structure comprises an air inlet and seed transfer pipe 4 which is inserted into the tank body from the top end of the tank body to the bottom of the tank body, and the air inlet and seed transfer pipe 4 exposed outside the tank body is sequentially connected with an air inlet structure, a steam sterilization structure, an inoculation sterilization structure and an inoculation structure; the air inlet structure is reversely connected with a sampling structure. The exhaust structure comprises an exhaust pipe 5 communicated with the inside of the tank body and an exhaust valve 6 arranged on the exhaust pipe 5. The cleaning structure comprises a cleaning pipe 7 communicated with the inside of the tank body and a cleaning valve 8 arranged on the cleaning pipe 7, and a cleaning ball 9 is arranged at the top end of the cleaning pipe in the tank body. The material pressing structure comprises a material pressing air pipe 10 communicated with the inside of the tank body and a material pressing valve 11 arranged on the material pressing air pipe 10. The air inlet structure comprises an air inlet pipe 12 communicated with the air inlet and seed transfer pipe 4 and an air inlet valve 13 arranged on the air inlet pipe; the sampling structure is reversely communicated with the air inlet pipe and comprises a sampling pipe 14 reversely communicated with the air inlet pipe, and a sampling valve 15 is arranged on the sampling pipe; the air inlet pipe and the material pressing air pipe are connected in parallel and then connected with an air pipe 16, and an air valve 17 and an air filter 18 are sequentially arranged on the air pipe 16. The steam sterilizing structure comprises a steam pipe 19 communicated with the air inlet and seed transferring pipe 4 and a steam valve 20 arranged on the steam pipe 19, a steam sterilizing valve 21 is arranged between the tail end of the steam pipe and the tail end of the air pipe, and the front end of the steam sterilizing structure is provided with a seed transferring valve 22. The inoculation and disinfection structure comprises an inoculation and disinfection steam pipe 23 communicated with the air inlet and the seed transfer pipe 4, an inoculation and disinfection valve 24 is arranged on the inoculation and disinfection steam pipe, and a clamp flange 25 is arranged at the front end of the inoculation and disinfection structure. The inoculating structure comprises an inoculating tube 26 communicated with the air inlet and the seed transferring tube 4 and an inoculating valve 27 arranged at the front end of the inoculating tube. The tank body is provided with a visual window 28; the outer side wall of the tank body is provided with a heat-insulating jacket layer 32; the bottom of the tank body is provided with a hydraulic lifting device 29 for vertically lifting the tank body. The lower end of the whole cultivating and seed transplanting device is provided with a universal wheel 30 which is convenient for the cultivating and seed transplanting device to horizontally move, and the universal wheel is provided with a brake structure.
The working principle is as follows:
Firstly, sterilizing an empty tank, sealing and covering a charging port 2 by a cover, connecting an air pipe 16 with an air source, temporarily avoiding air from being introduced, connecting a steam pipe 19 with the steam source, opening a steam sterilizing valve 21, slightly opening a filter blow-off valve 31 to discharge steam water, then opening an air valve 17, a material pressing valve 11, an air inlet valve 13 and an air outlet valve 6, sterilizing steam at the moment, entering the tank 1, sterilizing, discharging steam in the tank from the tank through the air outlet valve 6, observing the pressure displayed by a pressure sensor 3 and the temperature displayed by a thermometer 33, and adjusting the pressure in the tank by each valve. When the empty can is disinfected, the sampling valve 15, the seed shifting valve 22 and the cleaning valve 8 are slightly opened, and the three valves, the pipeline on which the valves are installed and the cleaning ball 9 are disinfected. After the disinfection of the empty can is finished, the valve which is opened outside the exhaust valve 6 is completely closed, then the air source is opened for ventilation, the air valve 17 is opened, the air inlet valve 13 is opened, and the positive pressure in the can is kept for standby.
When the culture medium is sterilized, the air inlet valve 13 is closed, and when the pressure in the tank is 0, the feed inlet 2 is opened, and the culture medium is placed in the tank body 1. The volume of the culture medium is determined by observation through the sight glass scale of the visual window 28, and then the feed inlet 2 is covered, screwed and sealed. Closing an air source, opening a steam sterilizing valve 21, slightly opening a filter blow-off valve 31 to drain steam water, then opening an air valve 17, an air inlet valve 13 and an air outlet valve 6, sterilizing steam at the moment enters the tank body 1 to sterilize, discharging the steam in the tank out of the tank body through the air outlet valve 6, observing the pressure gauge of the pressure sensor 3 to display pressure, and regulating the air inlet valve 13 to regulate the pressure in the tank. At this time, the culture medium materials begin to be disinfected, when the disinfection is finished according to the process requirement, the air inlet valve 13, the filter drain valve 31 and the steam disinfection valve 21 are sequentially closed, the air source is opened, the air enters the air pipe 16, then the air inlet valve 13 is opened, the air is introduced into the tank body 1, the positive pressure is kept in the tank body 1, the heat preservation jacket 32 is connected to constant temperature water to cool the culture medium, and the temperature of the constant temperature water is regulated to enable the temperature of the culture medium to reach the process requirement.
When the temperature of the culture medium reaches the process requirement temperature, a fire ring inoculation mode is used, strains are added into the tank body 1 from the feed inlet 2, and primary strain culture is carried out. In the strain culturing process, sampling can be performed by the sequence of opening the material pressing valve 11, closing the air inlet valve 13 and opening the sampling valve 15, and after sampling, the sequence of opening the air inlet valve 13, closing the material pressing valve 11 and closing the sampling valve 15 is finished.
After the strain is cultivated, the air pipe 16, the steam pipe 19, the heat-preserving jacket 32, the air source, the steam source and the constant temperature water are disconnected, the universal wheel 30 is unlocked to brake, the strain cultivating device is pushed to the vicinity of a secondary strain tank to be inoculated, and the proper height is adjusted through the hydraulic lifting device 29, so that the height of the hoop flange 25 is matched with the height of the inoculating pipe 26.
The air pipe 16 is connected to an air source, the steam pipe 19 and the inoculation sterilizing steam pipe 23 are connected to the steam source, the steam valve 20 is opened, the seed moving valve 22 is sterilized to the clamp flange 25, the inoculation sterilizing valve 24 is opened, the inoculation pipe 26 is sterilized, the clamp flange 25 is connected with the clamp flange at one end of the inoculation pipe 26 in a flame mode, the steam valve 20 and the inoculation sterilizing valve 24 are closed firstly during connection, and then the clamp flange 25 is connected under the condition of keeping flame. Then closing the air inlet valve 13, opening the material pressing valve 11, enabling the pressure in the tank body 1 to be higher than the pressure in the secondary seed tank, opening the seed shifting valve 22 and the inoculating valve 27, enabling strains to enter the secondary seed tank, observing the seed shifting amount according to the visual window 28, and closing the seed shifting valve 22 and the inoculating valve 27 after the seed shifting amount is reached, and finishing seed shifting.
Residual strains in the tank body 1 are discharged through the 15 sampling valve, the material pressing valve 11 is closed, the cleaning pipe 7 is connected to a cleaning water source, the cleaning valve 8 is opened to clean the tank body 1, the cleaning valve 8 is closed after cleaning is finished, the material pressing valve 11 and the sampling valve 15 are opened, the cleaning water in the tank body 1 is discharged, and the multifunctional seed transfer device for aseptic fermentation is in a standby state, and the second embodiment: the multifunctional seed culturing and transplanting device for aseptic fermentation comprises a tank body 1, wherein a feed inlet 2, a pressure sensor 3 for sensing the pressure in the tank body and a thermometer 33 for sensing the temperature of materials in the tank body are arranged at the upper end of the tank body, and the upper end of the tank body is connected with an exhaust structure, a cleaning structure, a material pressing structure, an air inlet and transplanting structure; the air inlet and seed transfer structure comprises an air inlet and seed transfer pipe 4 which is inserted into the tank body from the top end of the tank body to the bottom of the tank body, and the air inlet and seed transfer pipe 4 exposed outside the tank body is sequentially connected with an air inlet structure, a steam sterilization structure, an inoculation sterilization structure and an inoculation structure; the air inlet structure is reversely connected with a sampling structure. The exhaust structure comprises an exhaust pipe 5 communicated with the inside of the tank body and an exhaust valve 6 arranged on the exhaust pipe 5. The cleaning structure comprises a cleaning pipe 7 communicated with the inside of the tank body and a cleaning valve 8 arranged on the cleaning pipe 7, and a cleaning ball 9 is arranged at the top end of the cleaning pipe in the tank body. The material pressing structure comprises a material pressing air pipe 10 communicated with the inside of the tank body and a material pressing valve 11 arranged on the material pressing air pipe 10. The air inlet structure comprises an air inlet pipe 12 communicated with the air inlet and seed transfer pipe 4 and an air inlet valve 13 arranged on the air inlet pipe; the sampling structure is reversely communicated with the air inlet pipe and comprises a sampling pipe 14 reversely communicated with the air inlet pipe, and a sampling valve 15 is arranged on the sampling pipe; the air inlet pipe and the material pressing air pipe are connected in parallel and then connected with an air pipe 16, and an air valve 17 and an air filter 18 are sequentially arranged on the air pipe 16. The steam sterilizing structure comprises a steam pipe 19 communicated with the air inlet and seed transferring pipe 4 and a steam valve 20 arranged on the steam pipe 19, a steam sterilizing valve 21 is arranged between the tail end of the steam pipe and the tail end of the air pipe, and the front end of the steam sterilizing structure is provided with a seed transferring valve 22. The inoculation and disinfection structure comprises an inoculation and disinfection steam pipe 23 communicated with the air inlet and the seed transfer pipe 4, an inoculation and disinfection valve 24 is arranged on the inoculation and disinfection steam pipe, and a clamp flange 25 is arranged at the front end of the inoculation and disinfection structure. The inoculating structure comprises an inoculating tube 26 communicated with the air inlet and the seed transferring tube 4 and an inoculating valve 27 arranged at the front end of the inoculating tube. The tank body is provided with a visual window 28; the outer side wall of the tank body 1 is provided with a heat-insulating jacket 32; the bottom of the tank body is provided with a hydraulic lifting device 29 for vertically lifting the tank body.
Compared with the working principle of the first embodiment, the second embodiment is different in that: after the strain is cultivated, the connection between the air pipe 16 and the steam pipe 19 and the air source and the steam source is released, the strain cultivating device is pushed to the vicinity of the secondary strain tank to be inoculated in any mode, and the proper height is adjusted through the hydraulic lifting device 29, so that the height of the hoop flange 25 is matched with the height of the inoculating pipe 26. Other operations are the same as those of the first embodiment.
Embodiment III: referring to fig. 3, a multifunctional seed culturing and transplanting device for aseptic fermentation comprises a tank body 1, wherein a feed inlet 2, a pressure sensor 3 for sensing pressure in the tank body and a thermometer 33 for sensing material temperature in the tank body are arranged at the upper end of the tank body, and an exhaust structure, a cleaning structure, a material pressing structure, an air inlet and transplanting structure are connected at the upper end of the tank body; the air inlet and seed transfer structure comprises an air inlet and seed transfer pipe 4 which is inserted into the tank body from the top end of the tank body to the bottom of the tank body, and the air inlet and seed transfer pipe 4 exposed outside the tank body is sequentially connected with an air inlet structure, a steam sterilization structure, an inoculation sterilization structure and an inoculation structure; the air inlet structure is reversely connected with a sampling structure. The exhaust structure comprises an exhaust pipe 5 communicated with the inside of the tank body and an exhaust valve 6 arranged on the exhaust pipe 5. The cleaning structure comprises a cleaning pipe 7 communicated with the inside of the tank body and a cleaning valve 8 arranged on the cleaning pipe 7, and a cleaning ball 9 is arranged at the top end of the cleaning pipe in the tank body. The material pressing structure comprises a material pressing air pipe 10 communicated with the inside of the tank body and a material pressing valve 11 arranged on the material pressing air pipe 10. The air inlet structure comprises an air inlet pipe 12 communicated with the air inlet and seed transfer pipe 4 and an air inlet valve 13 arranged on the air inlet pipe; the sampling structure is reversely communicated with the air inlet pipe and comprises a sampling pipe 14 reversely communicated with the air inlet pipe, and a sampling valve 15 is arranged on the sampling pipe; the air inlet pipe and the material pressing air pipe are connected in parallel and then connected with an air pipe 16, and an air valve 17 and an air filter 18 are sequentially arranged on the air pipe 16. The steam sterilizing structure comprises a steam pipe 19 communicated with the air inlet and seed transferring pipe 4 and a steam valve 20 arranged on the steam pipe 19, a steam sterilizing valve 21 is arranged between the tail end of the steam pipe and the tail end of the air pipe, and the front end of the steam sterilizing structure is provided with a seed transferring valve 22. The inoculation and disinfection structure comprises an inoculation and disinfection steam pipe 23 communicated with the air inlet and the seed transfer pipe 4, an inoculation and disinfection valve 24 is arranged on the inoculation and disinfection steam pipe, and a clamp flange 25 is arranged at the front end of the inoculation and disinfection structure. The inoculating structure comprises an inoculating tube 26 communicated with the air inlet and the seed transferring tube 4 and an inoculating valve 27 arranged at the front end of the inoculating tube. The tank body is provided with a visual window 28; the outer side wall of the tank body 1 is provided with a heat-insulating jacket layer 32.
Compared with the working principle of the first embodiment, the third embodiment is different in that: after the strain is cultivated, the connection between the air pipe 16 and the steam pipe 19 and the air source and the steam source is released, the strain cultivating device is pushed to the vicinity of the secondary strain tank to be inoculated in any mode, and the height of the hoop flange 25 can be matched with the height of the inoculating pipe 26 in any mode. Other operations are the same as those of the first embodiment.
The above embodiments are only preferred embodiments of the present utility model, and are not intended to limit the scope of the present utility model, and any obvious modifications and substitutions are possible without departing from the scope of the utility model.
Claims (10)
1. The utility model provides a sterile fermentation is with multi-functional seed culture and move kind device, includes a jar body (1), jar body upper end is equipped with charge door (2), perception jar internal pressure's pressure sensor (3) and the internal material temperature's of perception jar thermometer, its characterized in that:
The upper end of the tank body is connected with an exhaust structure, a cleaning structure, a pressing structure, an air inlet and seed shifting structure;
The air inlet and seed shifting structure comprises an air inlet and seed shifting pipe (4) which is inserted into the tank body from the top end of the tank body to the bottom of the tank body, and the air inlet and seed shifting pipe (4) exposed outside the tank body is sequentially connected with an air inlet structure, a steam sterilization structure, an inoculation sterilization structure and an inoculation structure; the air inlet structure is reversely connected with a sampling structure.
2. The multifunctional seed culture and transplanting device for aseptic fermentation according to claim 1, wherein: the exhaust structure comprises an exhaust pipe (5) communicated with the inside of the tank body and an exhaust valve (6) arranged on the exhaust pipe (5).
3. The multifunctional seed culture and transplanting device for aseptic fermentation according to claim 1, wherein: the cleaning structure comprises a cleaning pipe (7) communicated with the inside of the tank body and a cleaning valve (8) arranged on the cleaning pipe (7), and a cleaning ball (9) is arranged at the top end of the cleaning pipe in the tank body.
4. The multifunctional seed culture and transplanting device for aseptic fermentation according to claim 1, wherein: the material pressing structure comprises a material pressing air pipe (10) communicated with the inside of the tank body and a material pressing valve (11) arranged on the material pressing air pipe (10).
5. The multifunctional seed culture and transplanting device for aseptic fermentation according to claim 1, wherein: the air inlet structure comprises an air inlet pipe (12) communicated with the air inlet and seed transfer pipe (4) and an air inlet valve (13) arranged on the air inlet pipe; the sampling structure is reversely communicated with the air inlet pipe and comprises a sampling pipe (14) reversely communicated with the air inlet pipe, and a sampling valve (15) is arranged on the sampling pipe; the air inlet pipe and the material pressing air pipe are connected in parallel and then connected with an air pipe (16), and an air valve (17) and an air filter (18) are sequentially arranged on the air pipe (16).
6. The multifunctional seed culture and transfer apparatus for aseptic fermentation according to claim 5, wherein: the steam sterilizing structure comprises a steam pipe (19) communicated with the air inlet and seed transferring pipe (4) and a steam valve (20) arranged on the steam pipe (19), a steam sterilizing valve (21) is arranged between the tail end of the steam pipe and the tail end of the air pipe, and the front end of the steam sterilizing structure is provided with the seed transferring valve (22).
7. The multifunctional seed culture and seed transfer apparatus for aseptic fermentation according to claim 6, wherein: the inoculation and disinfection structure comprises an inoculation and disinfection steam pipe (23) communicated with the air inlet and the seed transfer pipe (4), an inoculation and disinfection valve (24) is arranged on the inoculation and disinfection steam pipe, and a clamp flange (25) is arranged at the front end of the inoculation and disinfection structure.
8. The multifunctional seed culture and transfer apparatus for aseptic fermentation according to claim 7, wherein: the inoculation structure comprises an inoculation pipe (26) communicated with the air inlet and the seed transfer pipe (4) and an inoculation valve (27) arranged at the front end of the inoculation pipe.
9. The multifunctional seed culture and seed transfer apparatus for aseptic fermentation according to any one of claims 1 to 8, wherein: the tank body is provided with a visual window (28); the outer side wall of the tank body (1) is provided with a heat preservation jacket; the bottom of the tank body is provided with a hydraulic lifting device (29) for vertically lifting the tank body.
10. The multifunctional seed culture and seed transfer apparatus for aseptic fermentation according to any one of claims 1 to 8, wherein: the lower end of the whole cultivating and seed transferring device is provided with a universal wheel (30) which is convenient for the cultivating and seed transferring device to horizontally move, and the universal wheel is provided with a brake structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202323211199.6U CN221371174U (en) | 2023-11-27 | 2023-11-27 | Multifunctional seed culture and transplanting device for aseptic fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202323211199.6U CN221371174U (en) | 2023-11-27 | 2023-11-27 | Multifunctional seed culture and transplanting device for aseptic fermentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN221371174U true CN221371174U (en) | 2024-07-19 |
Family
ID=91863207
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202323211199.6U Active CN221371174U (en) | 2023-11-27 | 2023-11-27 | Multifunctional seed culture and transplanting device for aseptic fermentation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN221371174U (en) |
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2023
- 2023-11-27 CN CN202323211199.6U patent/CN221371174U/en active Active
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