CN220166134U - Single-chain preparation instrument - Google Patents

Abstract

The utility model provides a single-chain preparation instrument, which includes a support part, a pipetting tip head, a tip driving guide assembly, a pipetting part, a pipetting driving guide assembly, a liquid support part, a transverse driving guide assembly, and a vacuuming part. , multiple pipette tips are detachably installed on the pipette tip, the pipette tip, the pipette tip and the pipette part are connected, with suction and injection functions, and the pipette drive guide assembly drives the pipette part The suction head driving guide assembly drives the pipetting tip and pipetting part. There are multiple sets of workstations on the liquid support part. The transverse driving guide assembly connects and drives the liquid support part. The vacuum part connects and extracts the liquid support part. Liquid on the assembly station. This utility model replaces the traditional high-speed centrifugal force with the pneumatic drive of a miniature vacuum pump. The prepared single-stranded DNA does not contain large-volume and high-quality microbeads, and provides pure liquid phase raw materials for subsequent reactions such as downstream sequencing, which can ensure the accuracy of downstream experiments. Uniformity.

Description

A single chain preparation instrument

Technical field

The utility model belongs to the field of medical equipment, specifically a single-chain preparation instrument.

Background technique

In medical tests and experiments, it is often necessary to obtain DNA single-stranded products. However, the preparation steps for DNA single-stranded products are cumbersome, and there are strict requirements on the amount of reactants in each step. If inspection or experimental personnel only rely on conventional instruments such as test tubes, measuring cups, and pipettes to prepare DNA single-stranded products, it will lead to heavy workload, low work efficiency, and inevitable operating errors. Therefore, special instruments for preparing DNA single-stranded products are needed to assist workers in preparing DNA single-stranded products accurately and efficiently. Currently, using biotin-labeled DNA double-stranded products as starting materials to prepare single-stranded DNA is the mainstream technical route for product development. However, the single-stranded preparation process involves multiple high-speed centrifugation steps, which is difficult to automate due to the high centrifugal speed. When there are many samples, manual operations lead to low standardization. In addition, in the existing technology, microspheres are generally used to bind a single strand of DNA, and then the combination of the microsphere and the single strand of DNA is used as a raw material for subsequent reactions such as sequencing. However, the mass of the microspheres is relatively large and easy to deposit at the bottom of the solution, that is, it is not a pure liquid phase, which reduces the uniformity of subsequent reactions such as sequencing.

Utility model content

In view of the above-mentioned problems existing in the prior art, the purpose of this utility model is to provide a single-strand preparation instrument that can prepare DNA single-strand products based on PCR products. Each reactant corresponds to multiple work stations, using the pneumatic power of a miniature vacuum pump. The drive replaces traditional high-speed centrifugal force. The prepared single-stranded DNA does not contain large and high-quality microbeads. It provides pure liquid phase raw materials for downstream sequencing and other subsequent reactions, which can ensure the uniformity of downstream experiments.

In order to achieve the above purpose, the technical solution adopted by the present utility model is:

A single-chain preparation instrument, including a support part and a pipette tip installed on the support part, a tip drive guide assembly, a pipette part, a pipette drive guide assembly, a liquid support part, a transverse drive guide assembly, and a vacuum part, a plurality of pipetting tips are detachably installed on the pipetting tip, and the pipetting tip, the pipetting tip and the pipetting part are connected to form a structure with suction and injection functions, The pipetting drive guide assembly is connected to and drives the pipetting part to move for suction or injection. The tip driving guide assembly is connected to and drives the pipetting tip and the pipetting part to rise and fall. The liquid support part is located on the pipetting tip. Below, the liquid support part is provided with multiple sets of stations for installing reaction tubes and/or holding reactants required for preparing single chains. The transverse drive guide assembly is connected and drives the liquid support part to move linearly back and forth in the horizontal plane, pumping The vacuum part connects and extracts the liquid from a group of stations on the liquid support part.

As a further improvement of the above technical solution:

The liquid support part includes a supporting plate and a filter tip installation piece installed on the supporting plate. The supporting plate is provided with a positioning groove. The positioning groove includes a first groove and a second groove. The second groove is opened in the first groove. The groove on the bottom of the tank, the filter tip installation piece is provided with a plurality of filter tip installation stations for installing filter tips with filtering functions, and the filter tip installation stations are through holes that penetrate the filter tip installation piece , multiple filter tip installation stations are arranged in a row at parallel intervals. When the filter tip installation piece is installed on the pallet, the filter tip installation station is connected to the second groove, and the vacuuming part is connected to the second groove.

The side wall of the second groove is provided with a liquid suction hole which is a through hole and is used to connect the vacuum suction part.

The vacuuming part includes a waste liquid collection bottle, a vacuuming mechanism and a vacuuming pipe. The waste liquid collection bottle is connected to the vacuuming mechanism through a vacuuming pipe, and the waste liquid collection bottle is also connected to the second groove through another vacuuming pipe.

The liquid support part also includes a pallet assembly mounted on the pallet. The pallet is provided with at least one pallet positioning pin for positioning the pallet assembly. The pallet assembly is provided with multiple sets of workstations.

The workstations on the tray assembly include a first tank, a second tank, a plurality of sequencing tube positions, a plurality of mixed liquid levels and a plurality of denatured liquid levels. The first tank and the second tank are grooves opened on the upper surface of the pallet. , multiple sequencing tube positions are arranged in parallel and spaced in a row, multiple mixed liquid levels are arranged in parallel and spaced in a row, multiple denaturation liquid levels are arranged in parallel and spaced in a row, the length direction of the first tank, the length direction of the second tank, multiple The arrangement directions of the sequencing tube positions, the arrangement directions of the multiple mixing liquid positions, and the arrangement directions of the multiple denaturation liquid positions are parallel to each other.

The sequencing tube position, mixed liquid level and denaturing liquid level are all blind holes opened on the tray assembly. The sequencing tube position, mixed liquid level and denaturing liquid level are respectively used to install reaction tubes.

The pipetting tip head includes a pipetting tip installation piece, a cavity pressure plate and a first module connecting plate. The pipetting tip installation piece is provided with multiple pipetting tip installation positions, and the pipetting tip installation position is through Through the through hole of the pipetting tip mounting piece, multiple pipetting tip mounting positions are arranged in a row at parallel intervals. The upper end of the pipetting tip is removably installed at the bottom of the pipetting tip mounting position. The lower end of the pipetting tip Suspended downward, one end of the chamber pressure plate is connected to the pipetting tip installation piece, and the other end is connected to the first module connecting plate. The tip driving guide assembly is connected to and drives the first module connecting plate, the chamber pressure plate and the pipetting suction plate. The head mount raises or lowers.

The pipetting part includes a push rod pressure plate and a plurality of piston rods. The push rod pressure plate is located above the cavity pressure plate. One end of the push rod pressure plate is connected to a plurality of piston rods, and the other end is slidably provided on the first module connecting plate. The upper end of the piston rod is connected to the bottom of the push rod pressure plate, and the lower end is suspended downward. Multiple piston rods are arranged in a row at parallel intervals. The lower ends of the multiple piston rods are respectively inserted into multiple pipette tip installation positions. The pipetting drive guide assembly Connect and drive the push rod pressure plate to rise or fall, driving the piston rod to move along the pipetting tip installation position.

The single-chain preparation instrument also includes a controller and a control panel. The control panel is installed on the support part. The vacuum mechanism, the control panel, the suction head drive guide assembly, the pipetting drive guide assembly, and the transverse drive guide assembly are all integrated with the control unit. electrical connection.

The beneficial effects of the utility model are: DNA single-stranded products can be prepared based on PCR products, each reactant corresponds to multiple work stations, and the number of work stations used can be selected according to specific needs; the work stations corresponding to various reactants are arranged in an orderly manner , which improves the orderliness and accuracy of the preparation process; through the coordinated movement of the pipette tip and the liquid support part, the pipette tip can be aligned or inserted into different work stations; through the extraction and injection functions of the pipette part, different The liquid is transferred in the work station so that each solution reacts step by step to finally obtain the required single-chain product, and the single-chain preparation efficiency is high; each motor can be electrically connected to the controller and the reaction can be automated or semi-automated through programming. , the single-strand preparation instrument replaces the traditional high-speed centrifugal force with the pneumatic drive of a miniature vacuum pump. The prepared single-stranded DNA does not contain large and high-quality microbeads, and provides pure liquid phase raw materials for subsequent reactions such as downstream sequencing. Ensure the uniformity of downstream experiments.

Description of drawings

Figure 1 is a schematic structural diagram of an embodiment of the present utility model.

Figure 2 is a schematic diagram from another perspective of an embodiment of the present invention.

Figure 3 is a schematic diagram of one embodiment of the present invention with the push rod pressure plate removed.

Figure 4 is a schematic structural diagram of a pipetting tip mounting member according to an embodiment of the present invention.

Figure 5 is a schematic structural diagram of a supporting plate according to an embodiment of the present invention.

Figure 6 is a schematic structural diagram of a filter suction head installation component according to an embodiment of the present invention.

Figure 7 is a schematic structural diagram of a tray assembly according to an embodiment of the present invention.

Detailed ways

Specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

For the convenience of description, spatially relative terms can be used here, such as "on...", "on...", "on the upper surface of...", "above", etc., to describe what is shown in the figure. The spatial relationship between one device or feature and other devices or features. It will be understood that the spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. For example, if a feature in the figure is turned upside down, then one feature described as "above" or "on top of" other features or features would then be oriented "below" or "below" the other features or features. under other devices or structures". Thus, the exemplary term "over" may include both orientations "above" and "below." The device may be otherwise oriented (rotated 90 degrees or at other orientations) and the spatially relative descriptors used herein interpreted accordingly.

A single-chain preparation instrument, as shown in Figures 1 to 7, includes a support part and a pipette tip installed on the support part, a tip drive guide assembly, a pipette part, a pipette drive guide assembly, and a liquid support part 6. Lateral drive guide assembly, vacuuming part and control part.

The support part includes a bottom plate 11 and a vertical plate 12. The bottom plate 11 is supported on the ground or a workbench and is used to bear the weight of the entire instrument. One end of the vertical plate 12 is vertically connected to the middle of the bottom plate 11, and the other end hangs upward.

The pipetting tip head includes a pipetting tip mounting part 21, a cavity pressure plate 22, a first module connecting plate 23 and a second module connecting plate 24. The pipetting tip mounting part 21 is used to install the pipetting tip 101. The pipetting tip mounting part 21, the chamber pressure plate 22, the first module connecting plate 23 and the second module connecting plate 24 are connected in sequence.

The pipetting tip mounting piece 21 is provided with a plurality of pipetting tip mounting positions 211. The pipetting tip mounting positions 211 are through holes that penetrate the pipetting tip mounting piece 21. The plurality of pipetting tip mounting positions 211 are parallel. They are arranged in a row at intervals, and the center line of the pipette tip installation position 211 is perpendicular to the horizontal plane. The pipetting tip installation position 211 is a through hole with steps on the inner wall. The pipette tip 101 is detachably installed into the pipette tip installation position 211 from the bottom of the pipette tip installation position 211 . Specifically, the pipetting tip 101 is tapered as a whole, and the pipetting tip 101 has through holes penetrating its upper and lower ends. There is a suction nozzle at the bottom of the pipetting tip installation position 211. During installation, the pipetting tip 101 is placed outside the suction nozzle. The inner diameter of the upper end of the pipetting tip 101 and the outer diameter of the pipetting tip 101 are The diameters are the same, that is, there is no gap between the circumferential surface of the suction nozzle and the inner surface of the pipetting tip 101. The suction nozzle is provided with a mounting position 211 connecting the inside of the pipetting tip 101 and the liquid tip. of through holes. After the pipetting tip 101 is installed on the pipetting tip installation position 211, the upper end of the pipetting tip 101 is installed on the suction nozzle and the lower end hangs downward.

The cavity pressure plate 22 is plate-shaped, and its plane is parallel to the horizontal plane. One end of the chamber pressure plate 22 is connected to the pipette tip mounting member 21, and the other end is connected to the first module connecting plate 23. The middle portion of the end of the cavity pressure plate 22 close to the first module connecting plate 23 is concave toward the pipette tip mounting member 21 , forming a vertically penetrating shape with an opening on the side close to the first module connecting plate 23 .

The first module connecting plate 23 is plate-shaped, and its plane is perpendicular to the horizontal plane. One side of the first module connecting plate 23 is connected to the cavity pressure plate 22 , and the other side is connected to the second module connecting plate 24 . Preferably, the cavity pressure plate 22 is connected to the lower end of the first module connecting plate 23 . In order to improve the connection strength, connecting ribs 25 are provided, and the connecting ribs 25 connect the cavity pressure plate 22 and the first module connecting plate 23 at the same time.

Preferably, the cavity pressure plate 22 and the first module connection plate 23 are fixedly connected, and the first module connection plate 23 and the second module connection plate 24 are fixedly connected.

The suction head driving guide assembly includes a first motor 31 , a first screw rod 32 , a suction head slide rail 33 and two suction head slide blocks 34 . The first screw rod 32 is rotatably installed on a side of the vertical plate 12 close to the pipetting head, and the length direction of the first screw rod 32 is perpendicular to the horizontal plane. The first motor 31 is installed on the vertical plate 12 . The first motor 31 is located below the first screw 32 . The first motor 31 is connected to and drives the first screw 32 to rotate. A suction head slider 34 is screwed onto the first screw rod 32 . The suction head slide rail 33 is fixedly installed on the side of the vertical plate 12 close to the pipetting suction head. The suction head slide rail 33 and the first screw rod 32 are arranged in parallel and spaced apart. A suction head slide rail is slidably provided on the suction head slide rail 33. Block 34. The two suction head sliders 34 are connected to two ends of the second module connecting plate 24 respectively. When the first motor 31 drives the first screw rod 32 to rotate, the first screw rod 32 drives a suction head slider 34 to move linearly along the length direction of the first screw rod 32 , that is, to move perpendicularly to the horizontal plane. The suction head slider 34 drives the second module connecting plate 24 to rise and fall synchronously, that is, the entire pipetting suction head can be raised and lowered synchronously. The tip slide rail 33 and the other tip slider 34 play a role in guiding and supporting the lifting and balancing of the pipetting tip.

The pipetting part includes a push rod pressure plate 41 and a plurality of piston rods 42 . The push rod pressure plate 41 is plate-shaped and its plane is parallel to the horizontal plane. The push rod pressure plate 41 is located above the cavity pressure plate 22. One end of the push rod pressure plate 41 is connected to the first module connecting plate 23 and the other end is suspended. A plurality of piston rods 42 are fixedly connected to the bottom of the overhanging end of the push rod pressure plate 41 . The upper end of the piston rod 42 is connected to the bottom of the push rod pressure plate 41, and the lower end is suspended downward. A plurality of piston rods 42 are arranged in a parallel and spaced row. The aperture of the piston rod 42 is no larger than the aperture of the pipette tip installation position 211. Preferably, the aperture of the piston rod 42 is equal to the minimum aperture of the pipette tip installation position 211. Multiple piston rods 42 can be inserted into multiple pipettes respectively. In the pipetting tip mounting position 211 and along the length direction of the through hole on the pipetting tip mounting position 211 .

The pipetting drive guide assembly includes a drive assembly, two pressure plate slide blocks 52 and two pressure plate slide rails 53 . The two pressure plate slide rails 53 are arranged in parallel and spaced apart on the side of the first module connecting plate 23 close to the pipetting tip mounting part 21, and the pressure plate slide rails 53 are perpendicular to the horizontal plane. The two pressure plate slide blocks 52 are slidably disposed on the two pressure plate slide rails 53 respectively. The push rod pressure plate 41 is connected to the first module connecting plate 23 through two pressure plate slide blocks 52, and the push rod pressure plate 41 is connected to the pressure plate slide blocks 52. The driving part 51 is connected to and drives the push rod pressure plate 41 to rise and fall, and the cooperation of the pressure plate slide block 52 and the pressure plate slide rail 53 serves to guide the push rod pressure plate 41 . The driving assembly is installed on the first module connection plate 23 .

The driving assembly includes a second motor 511, a second screw 512 and a connecting block 513. The second motor 511 is installed on the first module connecting plate 23. The second motor 511 is connected to and drives the second screw 512 to rotate. The length direction of the rod 512 is perpendicular to the horizontal plane. The second screw rod 512 passes through the inner recess of the cavity pressure plate 2. The connecting block 513 is screwed to the second screw rod 512. The connecting block 513 is also connected to the cavity pressure plate 22. The block 513 is located between the two platen slide blocks 52 . The second motor 511 drives the second screw rod 512 to rotate. The second screw rod 512 drives the connecting block 513 to move along the length direction of the second screw rod 512. The connecting block 513 drives the push rod pressure plate 41 and the plurality of piston rods 42 to rise or fall.

The liquid support part 6 is located below the pipette tip 101 supported on the pipette tip installation position 211 . The liquid support part 6 includes a supporting plate 61 , a filter tip mounting part 62 and a tray assembly 63 . The supporting plate 61 is plate-shaped. The supporting plate 61 is provided with a positioning groove and two tray positioning pins 612 . The positioning groove and the two tray positioning pins 612 are located on the same surface of the supporting plate 61 . The positioning groove is used to install the filter tip mounting piece 62. The positioning groove is a long groove. Preferably, the positioning groove is a stepped groove, which is equivalent to two grooves, namely the first groove 610 and the second groove 611. The second groove 611 is opened at the third groove. There is a groove in the middle of the bottom surface of the groove 610. The length direction of the first groove 610 is parallel to the length direction of the second groove 611. A plurality of pin holes are provided on the steps of the positioning groove. In other words, pin holes are provided on the bottom surface of the first groove 610 on both sides of the second groove 611 . The second groove 611 is used to hold liquid. The side wall of the second groove 611 is provided with a liquid suction hole 613 which is a through hole.

The two tray positioning pins 612 are used to position the tray assembly 63, and the two tray positioning pins 612 are spaced apart. One end of the pallet positioning pin 612 is fixedly connected to the top surface of the pallet 61 and the other end is suspended upward. The connection line of the two tray positioning pins 612 is parallel to the length direction of the positioning groove. The cross section of a pallet positioning pin 612 is circular, that is, it is a cylindrical pin. The cross section of the other tray positioning pin 612 is a polygon, that is, a prism pin, and in this embodiment is a rhombus.

The filter tip mounting piece 62 is supported and installed in the positioning groove and is fixedly connected to the supporting plate 61 through the pin hole. After the filtering tip mounting piece 62 and the supporting plate 61 are connected, the contact surfaces of the two fit together to meet the air tightness requirements. The filter tip installation part 62 is provided with a plurality of filter tip installation stations 621. The filter tip installation stations 621 are used to install filter tips. The filter tip is provided with a filter element so that the filter tip has a filtering function. The filter tip installation station 621 is a through hole that penetrates the filter tip installation member 62. A plurality of filter tip installation stations 621 are arranged in a parallel row and the center line of the filter tip installation station 621 is perpendicular to the horizontal plane. The filter tip installation station 621 is a through hole with steps on the inner wall to stably support the filter tip. When the filter suction head is installed on the filter suction head installation station 621, the filter suction head is supported on the step. The filter tip installation part 62 is provided with numbers that mark the filter tip installation station 621 . When the filter suction head mounting member 62 is installed in the positioning groove, the filter suction head installation station 621 is connected to the second groove 611 . The contact surface between the filter tip mounting part 62 and the first groove 610 has good sealing performance.

The tray assembly 63 is supported on the pallet 61 . The bottom of the tray assembly 63 is provided with pin holes that cooperate with the two tray positioning pins 612 . The positioning and accurate installation of the tray assembly 63 is achieved through the cooperation of the pin holes and the tray positioning pins 612 . The tray assembly 63 is provided with a first tank 631, a second tank 632, a plurality of sequencing tube positions 633, a plurality of mixed liquid positions 634 and a plurality of denatured liquid levels 635. The first groove 631 and the second groove 632 are grooves formed on the upper surface of the supporting plate 61 . Steps are provided on the inner walls of the first groove 631 and the second groove 632 to serve as limit marks, or scale marks are provided on the inner walls of the first groove 631 and the second groove 632 . Multiple sequencing tube positions 633 are arranged in parallel and spaced in a row, multiple mixed liquid positions 634 are arranged in parallel and spaced in a row, and multiple denaturation liquid levels 635 are arranged in parallel and spaced in a row. The sequencing tube position 633 , the mixed liquid position 634 and the denaturing liquid position 635 are all blind holes opened on the tray assembly 63 . The pore sizes of the sequencing tube position 633, the mixing liquid position 634, and the denaturing liquid position 635 may be the same or different from each other. The length direction of the first groove 631, the length direction of the second groove 632, the arrangement direction of the plurality of sequencing tube positions 633, the arrangement direction of the plurality of mixed liquid positions 634, and the arrangement direction of the plurality of denaturation liquid positions 635 are parallel to each other. The first tank 631, the second tank 632, a row of sequencing tube positions 633, a row of mixed liquid positions 634 and a row of denaturing liquid levels 635 are arranged in sequence.

In this embodiment, the first tank 631 and the second tank 632 are used to contain pure water, the sequencing tube position 633 is used to install the sequencing reaction tube, the mixed liquid position 634 is used to install the mixed liquid tube, and the denaturation liquid level 635 is used to install the denaturant. liquid tube.

The transverse drive guide assembly is provided on the bottom plate 11 and is used to drive the liquid support part 6 to move linearly. The transverse drive guide assembly includes a third motor 71 , a conveyor belt 72 , a transverse slide block 73 and a transverse slide rail 74 . The transverse slide rail 74 is installed on the top surface of the base plate 11 . The length direction of the transverse slide rail 74 is perpendicular to the vertical plate 12 . The transverse slide block 73 is slidably disposed on the transverse slide rail 74 . The transverse slide block 73 is also connected to the bottom of the supporting plate 61 . The conveyor belt 72 is connected to two conveyor wheels spaced apart and arranged in the same plane. A third motor 71 is connected to and drives one of the conveyor wheels to rotate, driving the conveyor belt 72 to realize transmission. The transmission direction of the conveyor belt 72 is parallel to the transverse slide rail 74 . The bottom of the pallet 61 is also fixedly connected to the conveyor belt 72 through fasteners. The two conveyor wheels and the transverse slide rails 74 form a stable rigid quadrilateral, and the running speed of the conveyor wheels is very low, so the pallet 61 will not be unstable. . That is, the two ends of the bottom of the supporting plate 61 are respectively connected to the transverse slide block 73 and the conveyor belt 72. When the conveyor belt 72 is transmitted, the supporting plate 61 is driven to move linearly along the length direction of the transverse slide rail 74, and the moving direction is perpendicular to the length direction of the first groove 631. .

The vacuuming part includes a Schott bottle holder 81, a waste liquid collection bottle 82, a vacuuming mechanism 83 and a vacuuming tube. The Schott bottle holder 81 is fixedly arranged on the side of the vertical plate 12 away from the pipetting head, and the waste liquid collection bottle 82 is installed on the Schott bottle holder 81 . The vacuum mechanism 83 is installed on the bottom plate 11 and located below the waste liquid collection bottle 82 . The waste liquid collection bottle 82 is connected to the vacuum mechanism 83 through a vacuum pipe, and the waste liquid collection bottle 82 is also connected to the liquid suction hole 613 through another vacuum pipe. After the vacuum mechanism 83 is started, the liquid in the second groove 611 is pumped into the waste liquid collection bottle 82 . The waste liquid collection bottle 82, the vacuum mechanism 83, the vacuum tube and the second groove 611 form a sealing effect on one side of the filter tip installation station 621.

The vacuuming mechanism 83 can use a vacuum pump in the prior art.

The control part includes a controller and a control panel 91. The vacuum mechanism 83, each motor, and the control panel 91 are all electrically connected to the controller. The control panel 91 is set at one end of the base plate 11. The current status of the preparation instrument is displayed through the control panel 91. and perform operations. The technical solution for controlling the vacuuming mechanism 83 and each motor through the control panel 91 can adopt existing technology, and will not be described again here.

It can be seen from the above that the pipetting tip and the pipetting part can rise or fall, and the liquid support part 6 can move linearly back and forth in the horizontal plane.

Based on the above structure, the working principle and process of the utility model are:

Step 1: Install a filter tip on the filter tip installation station 621. The number of filter tips is determined as needed. Pour solution A into the filter tip. Solution A is the binding solution, PCR product and A mixture of microspheres. In this example, each filter tip contains a mixture of 38 ul of binding solution, 20 ul of PCR product and 2 ul of microspheres. When the vacuuming mechanism 83 is started, the substances in solution A that can penetrate the filter element in the filter tip are pumped into the waste liquid collection bottle 82. Specifically, the substances that penetrate the filter element first enter the second groove 611, and then are drawn from the vacuum pump. The liquid hole 613 is drawn into the waste liquid collection bottle 82 . PCR products and microspheres that are impermeable to the filter element are blocked by the filter element and remain on the surface of the filter element.

If the vacuum mechanism 83 is not activated, it is equivalent to the liquid suction hole 613 being sealed. At the same time, the contact surface between the filter tip mounting part 62 and the supporting plate 61 is airtight, which is equivalent to the lower end of the filter tip being sealed. , although the filter tip is a through hole and the filter element is permeable to liquid, the liquid above the filter element will not drop.

Add pure water to the first tank 631 and the second tank 632 to the step or scale. Add 30ul of denaturing liquid to the denaturing liquid tube at denaturing liquid level 635, add 6ul of annealing buffer and 1ul of sequencing primer to the mixing liquid tube at mixing liquid level 634, and add enzyme and substrate to the sequencing reaction tube at sequencing tube level 633.

Step 2: Use the vacuuming mechanism 83 to vacuum out the liquid in solution A that is permeable to the filter element, and the liquid in solution A that is permeable to the filter element is pumped to the waste liquid collection bottle 82 .

Step 3: Clean the material on the filter element in the filter tip, extract the water in the first tank 631 and the second tank 632 through the pipette tip 101, and clean the material left on the surface of the filter element in the filter tip. The fallen impurities and waste water pass through the filter element and are pumped into the waste liquid collection bottle 82.

Step 4: Use the pipette tip 101 to extract the denatured liquid in the denatured liquid level 635 into the filter tip on the filter tip installation station 621. Mix the denatured liquid and microspheres to obtain solution B. At this time, no When the vacuum mechanism 83 is started, the solution B will stay on the surface of the filter element without falling through the filter element.

Step 5: Prepare DNA single-stranded product: After allowing solution B to stand for a period of time, extract the supernatant C of solution B (which cannot contain microspheres), and transfer the supernatant C to the mixing liquid tube at the mixing liquid position 634. (the tube contains annealing buffer and sequencing primers), mix well to obtain mixture D.

Step 6: Use the pipette tip 101 to extract the mixed solution D into the sequencing tube position 633 to obtain a DNA single-stranded product.

The steps for extracting liquid from a container in the liquid support part 6 to another container through the pipetting tip 101 are as follows:

S1: The first motor 31 drives the pipetting tip mounting part 21 and the push rod pressing plate 41, so that the pipetting tip mounting part 21 and the pushing rod pressing plate 41 rise until the pipetting tip 101 is above the liquid support part 6, so as to The pipette tip 101 is prevented from interfering with the movement of the liquid support part 6 .

S2: The third motor 71 drives the liquid support part 6 so that the extraction liquid level is located directly below the plurality of pipetting tips 101 . Then, the first motor 31 drives the plurality of pipetting tips 101, so that the plurality of pipetting tips 101 are inserted into the extraction liquid level.

In this step, when the extraction liquid level is the first groove 631 and the second groove 632, multiple pipetting tips 101 are inserted into the first groove 631 and the second groove 632 at the same time. When the extraction liquid level is the sequencing tube level 633, the mixed liquid level 634, or the denatured liquid level 635, multiple pipetting tips 101 are inserted into the multiple sequencing tube positions 633, the multiple mixed liquid levels 634, or the denatured liquid level 635 respectively.

S3: The second motor 511 drives the push rod pressure plate 41 to rise. The push rod pressure plate 41 drives the plurality of piston rods 42 to rise, and draws the liquid in the extraction liquid level into the plurality of pipetting tips 101 .

S4: Same as S1, the pipetting tip 101 is driven by the first motor 31 to be positioned above the liquid support part 6 .

S5: The third motor 71 drives the liquid support part 6 so that the target liquid level is located directly below the plurality of pipetting tips 101.

S6: The second motor 511 drives and transmits the drive to the push rod pressure plate 41 to lower the push rod pressure plate 41. The push rod pressure plate 41 drives the plurality of piston rods 42 to drop, and the liquids in the multiple pipetting tips 101 are respectively injected into the target. in liquid level.

It can be seen from the above that in this solution, the target liquid level is the filter tip on the filter tip installation station 621 or the sequencing reaction tube on the sequencing tube position 633.

The transmission path through which the first motor 31 drives the pipette tip mounting member 21 and the push rod pressure plate 41 to rise and fall is: the first motor 31 drives the first screw rod 32 to rotate, and the first screw rod 32 is connected through the nut block and drives the second module. The connecting plate 24 rises or falls, and the second module connecting plate 24 drives the first module connecting plate 23, the chamber pressure plate 22, the pipetting tip mounting part 21, a plurality of pipetting tips 101, the push rod pressure plate 41, and the pressure plate. The slider 52 and the plurality of piston rods 42 rise or fall.

The transmission path of the second motor 511 driving the push rod pressing plate 41 is: the second motor 511 drives the second screw rod 512 to rotate, the second screw rod 512 is connected through the nut block and drives the connecting block 513 to rise or fall, and the connecting block 513 drives the push rod. The pressure plate 41 and the plurality of piston rods 42 rise or fall, and the pressure plate slide block 52 and the pressure plate slide rail 53 cooperate and guide.

It can be seen from the above that the piston rod 42, the pipette tip mounting position 211 and the pipette tip 101 form a syringe structure to realize the suction and injection functions. In order to ensure its functional effectiveness, one end of the piston rod 42 remains positioned at the pipette tip. In the installation position 211.

In this solution, the pipetting tip 101, the filter tip, and the sequencing reaction tube are used as consumables.

The above steps can be performed automatically or semi-automatically by setting a control program. By controlling the rotation distance of each motor, the moving distance of the pipetting tip 101, the moving distance of the liquid support part 6, and the amount of liquid extracted or injected by the pipetting tip 101 are controlled. , avoiding manual errors and improving preparation efficiency.

Finally, it is necessary to explain here that the above embodiments are only used to further explain the technical solution of the present utility model in detail and cannot be understood as limiting the protection scope of the present utility model. Some non-essential improvements and adjustments made belong to the protection scope of the present utility model.

Claims (10)

1. A single-strand preparation instrument, characterized in that it includes a support part and a pipette head mounted on the support part, a pipette drive guide assembly, a pipette part, a pipette drive guide assembly, a liquid support part (6), a transverse drive guide assembly, and a vacuum pumping part, wherein a plurality of pipette tips (101) are detachably mounted on the pipette head, the pipette tip (101), the pipette head and the pipette part are connected to form a structure with suction and injection functions, the pipette drive guide assembly is connected and drives the pipette part to move for suction or injection, the pipette drive guide assembly is connected and drives the pipette head and the pipette part to rise and fall, the liquid support part (6) is located below the pipette head, a plurality of groups of workstations for installing reaction tubes and/or holding reactants required for preparing single-stranded products are provided on the liquid support part (6), the transverse drive guide assembly is connected and drives the liquid support part (6) to move back and forth in a straight line in a horizontal plane, and the vacuum pumping part is connected and extracts liquid from a group of workstations on the liquid support part (6). 2. The single chain preparation instrument according to claim 1 is characterized in that: the liquid support part (6) includes a support plate (61) and a filter tip mounting member (62) installed on the support plate (61), a positioning groove is provided on the support plate (61), the positioning groove includes a first groove (610) and a second groove (611), the second groove (611) is a groove opened on the bottom surface of the first groove (610), and a plurality of filter tip mounting positions (621) for mounting filter tips with filtering functions are provided on the filter tip mounting member (62), the filter tip mounting position (621) is a through hole penetrating the filter tip mounting member (62), and the plurality of filter tip mounting positions (621) are arranged in a row in parallel and at intervals, and when the filter tip mounting member (62) is installed on the support plate (61), the filter tip mounting position (621) is communicated with the second groove (611), and the vacuum part is connected to the second groove (611). 3. The single chain preparation apparatus according to claim 2, characterized in that a liquid extraction hole (613) which is a through hole and is used to connect to the vacuum extraction part is provided on the side wall of the second groove (611). 4. The single chain preparation instrument according to claim 2 or 3 is characterized in that the vacuum pumping part includes a waste liquid collecting bottle (82), a vacuum pumping mechanism (83) and a vacuum pumping tube, the waste liquid collecting bottle (82) is connected to the vacuum pumping mechanism (83) through a vacuum pumping tube, and the waste liquid collecting bottle (82) is also connected to the second groove (611) through another vacuum pumping tube. 5. The single chain preparation apparatus according to claim 2 is characterized in that the liquid support part (6) further includes a tray assembly (63) mounted on a support plate (61), the support plate (61) is provided with at least one tray positioning pin (612) for positioning the tray assembly (63), and the tray assembly (63) is provided with multiple groups of workstations. 6. The single-strand preparation instrument according to claim 5 is characterized in that: the workstations on the tray assembly (63) include a first groove (631), a second groove (632), a plurality of sequencing tube positions (633), a plurality of mixed liquid positions (634) and a plurality of denaturing liquid positions (635); the first groove (631) and the second groove (632) are grooves provided on the upper surface of the support plate (61); the plurality of sequencing tube positions (633) are arranged in parallel and spaced apart in a row; the plurality of mixed liquid positions (634) are arranged in parallel and spaced apart in a row; the plurality of denaturing liquid positions (635) are arranged in parallel and spaced apart in a row; the length direction of the first groove (631), the length direction of the second groove (632), the arrangement direction of the plurality of sequencing tube positions (633), the arrangement direction of the plurality of mixed liquid positions (634) and the arrangement direction of the plurality of denaturing liquid positions (635) are parallel to each other. 7. The single-strand preparation instrument according to claim 6 is characterized in that the sequencing tube position (633), the mixing liquid level (634) and the denaturation liquid level (635) are all blind holes opened on the tray assembly (63), and the sequencing tube position (633), the mixing liquid level (634) and the denaturation liquid level (635) are respectively used to install reaction tubes. 8. The single chain preparation instrument according to claim 1 is characterized in that: the pipette head includes a pipette tip mounting part (21), a cavity pressure plate (22) and a first module connecting plate (23), and the pipette tip mounting part (21) is provided with a plurality of pipette tip mounting positions (211), the pipette tip mounting position (211) is a through hole that passes through the pipette tip mounting part (21), and the plurality of pipette tip mounting positions (211) are arranged in a row in parallel and spaced apart, the upper end of the pipette tip (101) is detachably mounted on the bottom of the pipette tip mounting position (211), and the lower end of the pipette tip (101) is cantilevered downward, one end of the cavity pressure plate (22) is connected to the pipette tip mounting part (21), and the other end is connected to the first module connecting plate (23), and the pipette tip driving guide component is connected and drives the first module connecting plate (23), the cavity pressure plate (22) and the pipette tip mounting part (21) to rise or fall. 9. The single-chain preparation instrument according to claim 8 is characterized in that: the pipetting part includes a push rod pressure plate (41) and multiple piston rods (42), the push rod pressure plate (41) is located above the cavity pressure plate (22), one end of the push rod pressure plate (41) is connected to multiple piston rods (42), and the other end is slidably arranged on the first module connecting plate (23), the upper end of the piston rod (42) is connected to the bottom of the push rod pressure plate (41), and the lower end is cantilevered downward, the multiple piston rods (42) are arranged in parallel and spaced apart in a row, the lower ends of the multiple piston rods (42) are respectively inserted into multiple pipette tip mounting positions (211), and the pipetting drive guide assembly is connected and drives the push rod pressure plate (41) to rise or fall, driving the piston rod (42) to move along the pipette tip mounting position (211). 10. The single-strand preparation instrument according to claim 1 is characterized in that: the single-strand preparation instrument also includes a controller and a control panel (91), the control panel (91) is installed on the support part, and the vacuum mechanism (83), the control panel (91), the tip drive guide assembly, the pipetting drive guide assembly, and the lateral drive guide assembly are all electrically connected to the controller.