CN220140431U - Special cutter Miao Die for plant tissue culture - Google Patents
Special cutter Miao Die for plant tissue culture Download PDFInfo
- Publication number
- CN220140431U CN220140431U CN202321077558.0U CN202321077558U CN220140431U CN 220140431 U CN220140431 U CN 220140431U CN 202321077558 U CN202321077558 U CN 202321077558U CN 220140431 U CN220140431 U CN 220140431U
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- miao
- die
- cutter
- box
- tissue culture
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- 238000004161 plant tissue culture Methods 0.000 title claims abstract description 20
- 238000009423 ventilation Methods 0.000 claims description 13
- 229920000742 Cotton Polymers 0.000 claims description 6
- 229920003023 plastic Polymers 0.000 claims description 6
- 244000005700 microbiome Species 0.000 claims description 4
- 238000005520 cutting process Methods 0.000 abstract description 37
- 238000000034 method Methods 0.000 abstract description 3
- 230000008569 process Effects 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 description 8
- 239000000463 material Substances 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 5
- 238000004659 sterilization and disinfection Methods 0.000 description 5
- 230000001954 sterilising effect Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 238000005299 abrasion Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 230000037039 plant physiology Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 230000017423 tissue regeneration Effects 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the field of plant tissue culture, in particular to a seedling cutting disc special for plant tissue culture. The utility model provides a dedicated cut Miao Die of plant tissue culture, includes cut Miao Die and aseptic box, cuts Miao Die and is circular dish form household utensils, cuts Miao Die and is provided with aseptic box, and aseptic box includes box body and lid, and aseptic box is used for placing and cuts Miao Die. By adopting the technical scheme of the utility model, the seedling cutting operation process is more convenient, the plant tissues are not easy to fall off during the seedling cutting, and the pollution to the plant tissues is reduced.
Description
Technical Field
The utility model relates to the field of plant tissue culture, in particular to a seedling cutting disc special for plant tissue culture.
Background
In recent 40 years, plant tissue culture technology has been rapidly developed, has penetrated into various research fields of plant physiology, pathology, pharmacy, genetics, breeding, biochemistry and the like, is widely applied to various industries of agriculture, forestry, industry, medicine industry and the like, generates huge economic benefit and social benefit, and becomes a most viable subject in the contemporary bioscience. In plant tissue culture, aseptic seedlings need to be cut, so that tissue regeneration culture is facilitated, and a cushion for cutting the seedlings is needed.
In order to solve the problems, chinese patent publication No. CN203167761U discloses a backing plate for cutting seedlings in tissue culture, which is made of stainless steel, two arc-shaped supporting legs are arranged on two sides of the lower part of the front end of the backing plate, a groove is formed in the upper part of the front end of the backing plate, the rear end of the backing plate is a smooth panel for cutting, and the number of the grooves is 2-3. The upper part of the front end of the utility model is provided with the groove, so that cut different explants can be conveniently placed, the rear end of the base plate is provided with the smooth cutting panel, the tissue culture seedling can be conveniently cut, two arc-shaped supporting legs are arranged on two sides of the lower part of the front end of the base plate, when a plurality of base plates are placed together, a space is reserved between the base plates, and air flow can be uniformly passed in the sterilization process, so that each base plate can be fully sterilized, and the utility model is convenient for repeated use.
The backing plate for cutting seedlings in tissue culture is made of stainless steel materials, although the backing plate is durable and wear-resistant, the cutting tool is easy to blunt due to the fact that the cutting tool touches the backing plate, meanwhile, generated noise is harsh, the backing plate is not friendly to a user, the arc-shaped supporting legs enable the panel to incline when being used normally, the operation is inconvenient, plant tissues roll off easily when the operation is improper, and the plant tissues are possibly polluted.
Disclosure of Invention
In order to solve the problems, the utility model aims to provide the special cutting Miao Die for plant tissue culture, which is more convenient in seedling cutting operation process, and the plant tissue is not easy to fall off during seedling cutting, so that the pollution to the plant tissue is reduced.
In order to achieve the above object, the technical scheme of the present utility model is as follows:
the utility model provides a dedicated cut Miao Die of plant tissue culture, includes cut Miao Die and aseptic box, cuts Miao Die and is circular dish form household utensils, cuts Miao Die and is provided with aseptic box, and aseptic box includes box body and lid, and aseptic box is used for placing and cuts Miao Die.
After the scheme is adopted, the following beneficial effects are realized:
the dish-shaped cutter Miao Die is large in opening, the bottom of the inner wall of the cutter Miao Die is flat, the seedling cutting operation is performed at the lower part of the inner wall of the cutter Miao Die, when the seedling cutting operation is performed, the upper part of the inner wall of the cutter Miao Die effectively prevents plant tissues from rolling to the outside, the cut plant tissues are contained in the seedling cutting dish in situ, the plant tissues are moved into the culture medium for cultivation after the whole seedling cutting operation is completed, the seedling cutting operation is more convenient, the step of temporarily storing the cut plant tissues into a special container is saved, and the pollution possibility of the plant tissues is reduced.
The dish-shaped seedling cutting dish is convenient for cutting Miao Die to stack, and the cutting Miao Die can be sterilized together with the culture medium, so that the resource waste is reduced; unused cut Miao Die is placed in an aseptic box, a box cover is arranged, and the aseptic box cover is covered to provide an aseptic environment for the cut Miao Die, so that the pollution of the cut Miao Die by the external environment is avoided, and the pollution risk of plant tissue culture is reduced.
Further, the slit Miao Die and the sterile box are all made of a non-toxic plastic that is transparent and resistant to high temperatures greater than 125 ℃.
The beneficial effects are that: the cut Miao Die and the aseptic box can be used after high-temperature sterilization, the sterilization temperature of the current plant culture medium is 121 ℃, and the cut Miao Die and the aseptic box which are made of materials resistant to the high temperature of 125 ℃ can be sterilized together with the plant culture medium, so that the resource waste is reduced; the transparent material is convenient for a user to observe the usage condition of the cut Miao Die and the sterile box; during seedling cutting operation, the cutter touches the cutter Miao Die, so that the abrasion of the cutter can be reduced by the plastic material, the replacement frequency of the cutter is reduced, and the cost is saved; meanwhile, the plastic material can reduce noise generated by touching the cutter with the cutter Miao Die, reduce influence of acoustic environment on a user, and ensure the stability of seedling cutting operation of the user; the nontoxic plastic can avoid the influence of the material of the cut Miao Die and the aseptic box on the seedling cutting operation.
Further, the bottom wall of the cutter Miao Die is provided with bulges which are uniformly distributed along the edge of the bottom wall, and the number of the bulges is at least three.
The beneficial effects are that: the cut Miao Die needs to be stacked for saving space, the cut Miao Die needs to be cleaned after being used, water or water vapor is also present on the surface of the non-drained cut seedling tray after being cleaned, a water film is easy to form between the stacked cut seedling trays, the tension is high, the cut seedling trays are adhered and not easy to separate, the bottom wall is provided with a bulge to separate the cut Miao Die from each other, and the adhesion between the cut seedling trays is reduced; the bulges are symmetrically distributed along the edge of the bottom wall, so that the stability of the placement of the cutter Miao Die is facilitated; at least three protrusions can guarantee the stability of the seedling cutting disc when placed.
Further, a handle is arranged at the top edge of the cutter Miao Die, the handle is a U-shaped handle, and at least one handle is arranged.
The beneficial effects are that: the handle is arranged to be convenient for the stress when taking the seedling cutting disc, and the user can move the cutting Miao Die smoothly.
Further, the aseptic box is rectangular.
The beneficial effects are that: the aseptic box is cuboid shape and makes cut seedling dish put into aseptic box back, and the four corners leaves the space in the box, and convenient to use person takes out and cuts Miao Die, and cuboid shape is convenient for accomodate.
Further, the box body is provided with a ventilation cylinder which communicates the inside of the sterile box with the external environment, and the number of the ventilation cylinders is at least one.
The beneficial effects are that: the ventilation cylinder is arranged to communicate the interior of the sterile box with the outside environment, and gas can circulate between the sterile box and the outside environment through the ventilation cylinder, so that the pressure change in the sterile box caused by the temperature change of the outside environment is avoided, the pressure stability in the sterile box is ensured, and the sterile box is not easy to deform; one ventilation cylinder can meet the requirement, and a plurality of ventilation cylinders can enable gas circulation to be more stable.
Further, cotton is plugged into the inside of the ventilation cylinder to isolate microorganisms in the external environment and keep the gas inside and outside the sterile box in circulation.
The beneficial effects are that: the breathable cylinder can be communicated with the aseptic box and the external environment, pollutants such as bacteria in the external environment can enter the aseptic box through the breathable cylinder to cause environmental pollution in the aseptic box, cotton is plugged into the breathable cylinder, microorganisms such as bacteria can be isolated, gas circulation is not affected, and the influence of the external environment on the internal environment of the aseptic box is reduced.
Further, the box cover is rotationally connected with the box body.
The beneficial effects are that: the box cover is rotationally connected with the box body, so that the box cover is widely applied to the market and is convenient for a user to open.
Drawings
FIG. 1 is a schematic diagram of an embodiment of the present utility model.
Fig. 2 is an overall schematic view of a seedling cutting disc according to an embodiment of the present utility model.
Fig. 3 is a schematic top view of an embodiment of the utility model Miao Die.
Fig. 4 is an overall schematic view of a sterile cassette according to an embodiment of the present utility model.
Detailed Description
The following is a further detailed description of the embodiments:
reference numerals in the drawings of the specification include: miao Die 1, handle 11, projection 12, aseptic box 2, box body 21, box cover 22, air permeable cylinder 23, cotton 24.
In the description of the present utility model, it should be understood that the terms "longitudinal," "transverse," "vertical," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the utility model and to simplify the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be configured and operated in a particular orientation, and thus should not be construed as limiting the utility model.
In the description of the present utility model, unless otherwise specified and defined, it should be noted that the terms "mounted," "connected," and "coupled" are to be construed broadly, and may be, for example, mechanical or electrical, or may be in communication with each other between two elements, directly or indirectly through intermediaries, as would be understood by those skilled in the art, in view of the specific meaning of the terms described above.
The embodiment is basically as shown in fig. 1 and fig. 2, and the special cutter Miao Die 1 for plant tissue culture comprises a cutter Miao Die 1 and a sterile box 2, wherein the cutter Miao Die 1 and the sterile box 2 are both made of transparent plastic with the temperature resistance of more than 125 ℃, and the embodiment is made of modified high-transparency PET material with the temperature resistance of 150 ℃; the cutter Miao Die is a circular dish-shaped vessel, the diameter of the top opening of the cutter Miao Die 1 is 18 cm, the diameter of the flat area at the lower part of the inner wall of the cutter Miao Die 1 is 13 cm, and the diameter of the bottom wall of the cutter Miao Die 1 is 13 cm; the handles 11 are arranged at the top edge of the cutter Miao Die, the handles 11 are U-shaped, two handles 11 are arranged in the embodiment, the handles 11 extend upwards along the inner wall of the seedling cutting disc 1 and the outer wall of the seedling cutting disc 1, the two handles 11 are symmetrical, and the handles 11 and the cutter Miao Die are integrally formed; the bottom wall of the cutter Miao Die is provided with the protrusions 12, the protrusions 12 are uniformly distributed along the edge of the bottom wall, the number of the protrusions 12 is at least three, and the three protrusions 12 are selected and integrally formed with the bottom wall in the embodiment.
In the embodiment, the cutter Miao Die 1 is provided with a sterile box 2, and the sterile box 2 is used for placing the cutter Miao Die 1; the aseptic box 2 is cuboid, the aseptic box 2 is cube-shaped in the embodiment, and the aseptic box 2 comprises a box body 21 and a box cover 22; the side wall of the box body 21 is provided with the ventilation cylinders 23, the ventilation cylinders 23 communicate the inside of the sterile box 2 with the outside environment, the number of the ventilation cylinders 23 is at least one, and the number of the ventilation cylinders 23 is two in the embodiment, so that the ventilation of gas is enhanced, and the temperature stability in the box body 21 is maintained; cotton 24 is plugged into the breathable cylinder 23, and the cotton can isolate microorganisms in the external environment and keep gas circulation inside and outside the sterile box; the box cover 22 is rotatably connected with the box body 21.
The unused cut Miao Die 1 is put into the sterilized sterile box 2 after being sterilized, a plurality of seedling cutting discs 1 are stacked, and the box cover 22 and the box body 21 are kept in a covering state after the placement is finished; before the seedling cutting operation, taking out the cut Miao Die 1 from the aseptic box 2, putting plant tissues into the seedling cutting disc 1, and performing the seedling cutting operation on the lower part of the bottom inner wall of the seedling cutting disc 1; after the seedling cutting operation is completed, plant tissues are moved into a culture medium for cultivation, the used cut Miao Die 1 is cleaned, and the plant tissues are placed into a sterile box 2 for standby after disinfection and sterilization.
The foregoing is merely exemplary embodiments of the present utility model, and specific structures and features that are well known in the art are not described in detail herein. It should be noted that modifications and improvements can be made by those skilled in the art without departing from the structure of the present utility model, and these should also be considered as the scope of the present utility model, which does not affect the effect of the implementation of the present utility model and the utility of the patent. The protection scope of the present utility model is subject to the content of the claims, and the description of the specific embodiments and the like in the specification can be used for explaining the content of the claims.
Claims (7)
1. A special cutter Miao Die for plant tissue culture, which is characterized in that: the novel sterile box comprises a cutter Miao Die and a sterile box, wherein the cutter Miao Die is a circular dish-shaped vessel, the cutter Miao Die is provided with the sterile box, the sterile box comprises a box body and a box cover, and the sterile box is used for placing the cutter Miao Die; the cut Miao Die and the sterile box are all made of transparent plastic which can resist high temperature of more than 125 ℃.
2. The plant tissue culture specific cutter Miao Die according to claim 1, wherein: the bottom wall of the cutter Miao Die is provided with bulges which are uniformly distributed along the edge of the bottom wall, and the number of the bulges is at least three.
3. The plant tissue culture specific cutter Miao Die according to claim 1, wherein: the top edge of the cutter Miao Die is provided with at least one handle, wherein the handle is a U-shaped handle.
4. The plant tissue culture specific cutter Miao Die according to claim 1, wherein: the aseptic box is cuboid.
5. The plant tissue culture specific cutter Miao Die according to claim 1, wherein: the box body is provided with ventilative drum, ventilative drum communicates aseptic box inside and external environment, ventilative drum quantity is at least one.
6. The plant tissue culture specific cutter Miao Die according to claim 5, wherein: cotton is plugged into the breathable cylinder, so that microorganisms in the external environment are isolated, and the ventilation of the inside and outside of the sterile box is kept.
7. The plant tissue culture specific cutter Miao Die according to claim 1, wherein: the box cover is rotationally connected with the box body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321077558.0U CN220140431U (en) | 2023-05-08 | 2023-05-08 | Special cutter Miao Die for plant tissue culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321077558.0U CN220140431U (en) | 2023-05-08 | 2023-05-08 | Special cutter Miao Die for plant tissue culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220140431U true CN220140431U (en) | 2023-12-08 |
Family
ID=89019205
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321077558.0U Active CN220140431U (en) | 2023-05-08 | 2023-05-08 | Special cutter Miao Die for plant tissue culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220140431U (en) |
-
2023
- 2023-05-08 CN CN202321077558.0U patent/CN220140431U/en active Active
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