CN219935373U - Biological sample tissue free water tritium extraction and collection device - Google Patents
Biological sample tissue free water tritium extraction and collection device Download PDFInfo
- Publication number
- CN219935373U CN219935373U CN202320327992.3U CN202320327992U CN219935373U CN 219935373 U CN219935373 U CN 219935373U CN 202320327992 U CN202320327992 U CN 202320327992U CN 219935373 U CN219935373 U CN 219935373U
- Authority
- CN
- China
- Prior art keywords
- biological sample
- tritium
- free water
- collection device
- vacuum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 99
- YZCKVEUIGOORGS-NJFSPNSNSA-N Tritium Chemical compound [3H] YZCKVEUIGOORGS-NJFSPNSNSA-N 0.000 title claims abstract description 88
- 229910052722 tritium Inorganic materials 0.000 title claims abstract description 88
- 239000012472 biological sample Substances 0.000 title claims abstract description 66
- 238000000605 extraction Methods 0.000 title claims abstract description 31
- 238000001291 vacuum drying Methods 0.000 claims abstract description 47
- 239000007788 liquid Substances 0.000 claims abstract description 25
- 239000000110 cooling liquid Substances 0.000 claims description 33
- 239000000523 sample Substances 0.000 claims description 26
- 239000002274 desiccant Substances 0.000 claims description 9
- 238000001035 drying Methods 0.000 claims description 7
- 238000005485 electric heating Methods 0.000 claims description 3
- 238000009833 condensation Methods 0.000 abstract description 16
- 230000005494 condensation Effects 0.000 abstract description 16
- 238000010438 heat treatment Methods 0.000 abstract description 10
- 238000001704 evaporation Methods 0.000 abstract description 5
- 230000008020 evaporation Effects 0.000 abstract description 5
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 2
- 238000004458 analytical method Methods 0.000 description 12
- 238000000034 method Methods 0.000 description 11
- 238000012360 testing method Methods 0.000 description 11
- 230000005855 radiation Effects 0.000 description 9
- WYTGDNHDOZPMIW-RCBQFDQVSA-N alstonine Natural products C1=CC2=C3C=CC=CC3=NC2=C2N1C[C@H]1[C@H](C)OC=C(C(=O)OC)[C@H]1C2 WYTGDNHDOZPMIW-RCBQFDQVSA-N 0.000 description 7
- 238000001816 cooling Methods 0.000 description 7
- 238000001514 detection method Methods 0.000 description 7
- 238000012544 monitoring process Methods 0.000 description 7
- 239000011521 glass Substances 0.000 description 6
- 239000007789 gas Substances 0.000 description 5
- 239000003960 organic solvent Substances 0.000 description 5
- 238000010533 azeotropic distillation Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000009489 vacuum treatment Methods 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
- 239000002826 coolant Substances 0.000 description 3
- 239000012809 cooling fluid Substances 0.000 description 3
- 230000007797 corrosion Effects 0.000 description 3
- 238000005260 corrosion Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000011835 investigation Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 244000271437 Bambusa arundinacea Species 0.000 description 2
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 2
- 235000011613 Pinus brutia Nutrition 0.000 description 2
- 241000018646 Pinus brutia Species 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 230000018044 dehydration Effects 0.000 description 2
- 238000006297 dehydration reaction Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000000967 suction filtration Methods 0.000 description 2
- 239000010409 thin film Substances 0.000 description 2
- 238000009777 vacuum freeze-drying Methods 0.000 description 2
- XDTMQSROBMDMFD-UHFFFAOYSA-N Cyclohexane Chemical compound C1CCCCC1 XDTMQSROBMDMFD-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 231100000045 chemical toxicity Toxicity 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000004508 fractional distillation Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000004630 mental health Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000003595 mist Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000010815 organic waste Substances 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003828 vacuum filtration Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 239000008096 xylene Substances 0.000 description 1
Landscapes
- Sampling And Sample Adjustment (AREA)
Abstract
The utility model discloses a biological sample Tissue Free Water Tritium (TFWT) extraction and collection device, which is characterized in that a vacuum drying box, a condensation component, a suction filter connector and a vacuum pump are sequentially connected, a constant-temperature heating and negative-pressure environment is provided by the vacuum drying box to directly heat and suck a biological sample at the same time, so that the evaporation of TFWF in the biological sample is accelerated, TFWT steam is condensed into liquid tritium water through the condensation component and flows out of the suction filter connector, and finally the liquid tritium water is collected by a tritium water collection box connected to the bottom end of the suction filter connector, thereby the tissue free water tritium in the biological sample is extracted rapidly and efficiently, and no chemical reagent is introduced into the biological sample in the whole extraction process, so that the extraction device is safer and more convenient.
Description
Technical Field
The utility model belongs to the technical field of radiation environment monitoring and investigation, and particularly relates to a device for extracting and collecting free water tritium from biological sample tissues.
Background
Tritium is a natural radionuclide widely existing in the environment, and is also an important artificial radionuclide in the field of nuclear industry, and the tritium participates in the global hydrogen circulation and all biological metabolic processes and irradiates organisms with radiation. Tritium in the environment exists primarily in the form of gaseous tritium, tritium water, or organic tritium in the atmosphere, in water bodies, and in organisms, wherein the organisms exist primarily in the form of organized free water tritium (TFWT) and Organically Bound Tritium (OBT). The detection analysis of The Free Water Tritium (TFWT) and the organically combined tritium (OBT) of the tissue in the organism is definitely regulated in relevant specifications such as radiation environment monitoring technical Specification (HJ 61-2021), radiation environment background investigation technical Specification before the operation of the nuclear power plant (HJ 969-2018) and the like in the process of radiation environment quality monitoring, radiation background investigation monitoring before the operation of the nuclear power plant, radiation environment monitoring during the operation of the nuclear power plant and the like. The detection and analysis method is implemented by referring to the analysis method of tritium in water (HJ 1126-2020), and the standard only provides for detection and analysis of the tritium content in a water sample containing tritium or in a liquid effluent, but no clear regulation is seen how to extract and collect Tissue Free Water Tritium (TFWT) in organisms and convert the tissue free water tritium into tritium water which can be detected by the analysis method of tritium in water (HJ 1126-2020).
At present, TFWT in organisms is usually separated by freeze dehydration, vacuum freeze drying, azeotropic distillation and other methods at home and abroad, wherein the former two methods can obtain higher recovery rate (more than 90 percent) of TFWT by continuous operation of a professional freeze dryer for 2-3 days, and biological samples are required to be frozen and decomposed before the operation of the instrument so as to meet the requirements of sample split charging and freeze drying. The azeotropic distillation method is to break up the biological sample, then put the broken biological sample into organic solvent, carry out TFWT in the organism by heating and distilling the organic solvent, and realize TFWT separation by fractional distillation. Organic solvents with a low azeotropic point and a high water content of the azeotropic point, such as benzene, toluene, xylene, cyclohexane, etc., are generally selected by azeotropic distillation, and the method can complete the extraction and separation of the biological TFWT in several hours, but has a low recovery rate (generally less than 70%). Because the organic solvent is volatile and has chemical toxicity, the organic solvent inevitably has certain harm to the health of the testers. In addition, the method can generate a certain amount of organic waste liquid, and the separated tritium water can contain a small amount of organic solution so as to influence the analysis and measurement results of the sample.
The Chinese patent publication No. CN 210442151U discloses a sampling device for free water tritium in tissue of biological sample, which is based on distillation principle, heats the sample to be measured by a low-temperature analysis furnace to obtain tritium-containing vapor, and the vapor is led out to a collecting pipe by a glass pipe, and is collected under the condensation of a cold trap. The TFWT in the pine needle leaf and the common bamboo root leaf are analyzed by the device in the embodiment 1 of the specification, the analysis temperature is 105 ℃, and the collection efficiency of the pine needle leaf and the common bamboo root leaf TFWT is 78% and 82% respectively.
Meng Dan et al in the publication of journal nuclear electronics and detection technology, "low temperature analysis-liquid flash method for detecting tritium content in plants," disclose a sampling device similar to the above patent, and when such a device processes a biological sample, it is usually necessary to break the biological sample, freeze it, and put it into an analysis glass tube, the pretreatment is complicated, and the amount of sample that can be analyzed at one time is small, for example, the analysis of biological samples with lighter quality (such as vegetables and crop branches and leaves) is required to be performed multiple times to obtain a sufficient amount of TFWT.
Therefore, there is a need to develop a device for extracting and collecting free water tritium from biological sample tissue, which is rapid, efficient and convenient and safe to use.
Disclosure of Invention
The utility model aims to provide a device for extracting and collecting free water tritium from biological sample tissues, which is rapid and efficient in extraction and collection and convenient and safe in use.
In order to achieve the purpose of the utility model, the utility model adopts the following technical scheme:
a biological sample tissue free water tritium extraction and collection device, comprising:
the vacuum drying box is provided with a vacuum valve and a gas release valve;
a condensing assembly comprising a condensing tube having an inlet and an outlet, the inlet being connected to the vacuum valve;
the suction filter connector comprises a connector body, wherein a cavity is formed in the connector body in a hollow mode, interfaces are arranged at the top end, the bottom end and the side wall of the connector body, the interfaces are communicated with the cavity, and the interface at the top end of the connector body is connected with the outlet of the condenser pipe;
the tritium water collector is connected with the bottom end of the connector body;
and the vacuum pump is connected with an interface of the side wall of the connector body, so that the vacuum pump is sequentially connected with the suction filter connector, the condensing tube and the vacuum drying box.
Preferably, the suction filter connector is a water vapor filter, and a filter element is also accommodated in the cavity.
Preferably, the device further comprises a dryer, one end of the dryer is connected with the interface of the side wall of the connector body, and the other end of the dryer is connected with the vacuum pump.
Preferably, the condensing assembly further comprises a low-temperature cooling liquid circulating pump, the low-temperature cooling liquid circulating pump is provided with a cooling liquid inlet and a cooling liquid outlet, the condensing tube comprises an inner tube and an outer tube, the inner tube is sleeved in the outer tube, the outer tube is provided with a liquid inlet and a liquid outlet, the liquid inlet is communicated with the cooling liquid outlet, and the liquid outlet is communicated with the cooling liquid inlet.
Preferably, the condenser tube is a serpentine condenser tube, and the serpentine condenser tube is obliquely arranged. The inclined arrangement can be realized by further installing a fixing frame or other conventional fixing modes.
Preferably, the suction filter connector and the dryer are both arranged vertically, and the interface of the side wall of the connector body is arranged close to the top end. The upright arrangement may be achieved by further mounting of a mount or the like in a conventional manner.
Optionally, the vacuum drying oven is a constant temperature electric heating vacuum drying oven, a plurality of sample chambers are arranged in the drying oven, and the temperature control range of the sample chambers is room temperature +10 ℃ to 200 ℃.
Optionally, the vacuum pump is a rotary vane vacuum pump.
Optionally, the dryer comprises a straight through pipe with two ends communicated, and the drying agent is contained in the pipe.
Preferably, the use temperature of the cryogenic cooling fluid circulation pump is set to 4 ℃.
Compared with the prior art, the utility model has the beneficial effects that at least the following steps are included:
1. according to the biological sample tissue free water tritium extraction and collection device, the vacuum drying box, the condensation component, the suction filter connector and the vacuum pump are sequentially connected, the environment of constant temperature heating and negative pressure provided by the vacuum drying box is utilized to directly heat and suck the biological sample at the same time, so that evaporation of TFWF in the biological sample is accelerated, TFWT steam is condensed into liquid tritium water through the condensation component, and the liquid tritium water flows out of the suction filter connector and is collected by the tritium water collection box connected to the bottom end of the suction filter connector, thereby the tissue free water tritium in the biological sample is extracted rapidly and efficiently, no chemical reagent is introduced into the biological sample in the whole extraction process, on one hand, physical and mental health and safety of test personnel are facilitated, and on the other hand, the cost is saved;
2. in the biological sample tissue free water tritium extraction and collection device provided by the utility model, the vacuum drying box has high inclusion degree on biological samples, the cleaned biological samples are only required to be laid in the tray and put in the sample chamber of the vacuum drying box, the biological samples are not required to be divided and crushed into fine particles or grains, the vacuum drying box with proper volume can be selected according to the actual sample condition, or the vacuum drying box with larger volume is selected, so that the extraction requirements of TFWT (thin film wt) in different biological samples can be met at one time, namely, enough biological TFWT water tritium can be extracted for subsequent test analysis only by carrying out one time of test, the use is convenient and efficient, the sample processing time is saved, and the practicability is good;
3. according to the utility model, the cooling assembly comprises the condensing pipe and the low-temperature cooling liquid circulating pump, the cooling liquid is circularly conveyed into the outer pipe of the condensing pipe through the low-temperature cooling liquid circulating pump to condense tritium-containing steam in the inner pipe, the low-temperature cooling liquid circulating pump is convenient for controlling the actual cooling temperature, and the TFWT steam can be effectively condensed without being frozen into ice to influence the flow property of a pipeline, so that the use safety of the device is further improved;
4. in the utility model, the suction filter connector can be preferably a water vapor filter, namely, a filter element is also accommodated in the inner cavity of the suction filter connector, the water vapor filter not only can filter tritium water flowing out of the condenser pipe, but also has the functions of liquefying vapor and fog, and discharges filtered air, so that the collection efficiency of the TFWT can be further improved, and when the side wall interface of the suction filter connector is connected with the dryer, the replacement frequency of a drying agent in the dryer can be reduced, and the vacuum pump is protected from being corroded by water vapor;
5. in the device for extracting and collecting the free water tritium of the biological sample tissue, the side wall interface of the suction filter connector can be further connected with a dryer, so that the air exhausted by the suction filter connector is further dried, and the corrosion to a vacuum pump is reduced.
Drawings
Fig. 1: the embodiment of the utility model provides a schematic diagram of a free water tritium extraction and collection device for biological sample tissues.
In the figure: 10. a vacuum drying box 101, a vacuum valve 102 and a gas release valve;
201. a condensing pipe 2011, an inner pipe 202 and a low-temperature cooling liquid circulating pump;
30. a suction filter connector 301, a top end interface 302, a bottom end interface 303 and a side wall interface;
40. a tritium water collector;
50. a vacuum pump;
60. a connecting pipe;
70. dryer, 701, drier.
Detailed Description
In the prior art, the technical problems that the pretreatment of the biological sample is complex, the device is not convenient, safe and efficient enough for extracting and collecting free water tritium of the biological sample tissue, and further improvement is needed exist.
The utility model is therefore to be considered in all respects as illustrative and not restrictive, and the utility model will be described in detail with reference to the accompanying drawings and examples.
The utility model provides a device for extracting and collecting free water tritium from biological sample tissues, which is shown by referring to fig. 1, and comprises a vacuum drying oven 10, a condensation assembly, a suction filtration connector 30, a tritium water collector 40 and a vacuum pump 50 which are connected in sequence. Wherein the vacuum drying oven 10 is provided with a vacuum valve 101 and a gas release valve 102, the condensation assembly comprises a condensation pipe 201, the condensation pipe 201 is provided with the inlet and the outlet, and the inlet is connected with the vacuum valve 101; the suction filter connector 30 comprises a connector body, a cavity (not labeled in the figure) is formed in the hollow structure of the connector body, interfaces (namely, a top end interface 301, a bottom end interface 302 and a side wall interface 303) are arranged at the top end, the bottom end and the side wall of the connector body, the interfaces are communicated with the cavity, the interface (namely, the top end interface 301) at the top end of the connector body is connected with the outlet of the condenser tube 201 and is used for receiving tritium water condensed out of the condenser tube 201, and the tritium water flows into the cavity through the top end interface 301 and flows out of the bottom end interface 302. The tritium water collector 40 is connected with the bottom end interface 302 of the suction filter connector 30 and is used for collecting tritium water obtained after condensation, and the collector can be a common container and is made of glass or quartz; the vacuum pump 50 is connected to the side wall interface (i.e., the side wall interface 303) of the connector body, so that the vacuum pump 50 is sequentially connected to the suction filter connector 30, the condenser pipe 201, and the vacuum drying oven 10, so as to perform vacuum treatment on the vacuum drying oven 10, the condenser pipe 201, and the suction filter connector 30.
The technical principle adopted by the utility model is that TFWT steam is obtained by accelerating the evaporation of TFWT in a biological sample by simultaneously acting on the biological sample in a heating and sucking mode, and the TFWT steam is converted into liquid tritium water for collection after being cooled at a low temperature. Aiming at the quality and density differences of different biological samples, the utility model adopts the design concept of a vacuum drying box to increase the sample capacity, and simultaneously provides a working environment with constant temperature heating and negative pressure for the biological samples through the vacuum drying box so as to improve the TFWT evaporation rate in the biological samples, promote the rapid drying of the biological samples, and simultaneously avoid the external gas from entering the drying box to further influence the composition and content of tritium water.
In the present utility model, the vacuum drying oven 10 can be a commercially available product, which is a traditional and mature drying device, and is often applied to the industries of pharmacy, chemical industry, food, etc., and has not been found in the related report of applying the vacuum drying oven to the extraction and collection field of tissue free water tritium in biological samples. The commercially available vacuum drying oven 10 is generally provided with a box body, a plurality of sample chambers for placing samples are arranged in the box body, the sample chambers can be separated by a plurality of partition boards, the box body is also provided with a vacuum valve 101 and a deflation valve 102, the vacuum valve 101 and the deflation valve 102 are communicated with the sample chambers, so that the vacuum treatment can be performed on the sample chambers through the vacuum valve 101, the vacuum treatment is performed on the sample chambers after the vacuum treatment through the deflation valve 102 and the external ventilation, and the vacuum meter is further arranged on the surface of the box body, so that the vacuum degree in the sample chambers can be reacted in real time. As an example, the vacuum drying oven 10 of the present utility model may be a constant temperature electric heating vacuum drying oven, such as an intelligent temperature control vacuum drying oven (DZF series) manufactured by Shanghai-constant scientific instrument, and more specifically, the specific parameters of the vacuum drying oven may be: the working environment temperature of the vacuum drying box is 5-40 ℃, the temperature control range in the sample chamber is between Room Temperature (RT) +10-200 ℃, the temperature fluctuation degree is 1 ℃, and the maximum vacuum degree in the sample chamber can reach 133pa.
The water content of the biological samples of different types is generally about 60-90%, and the amount of tritium water required in the radiation detection process is about 50g, and the inventor finds that the biomass required for TFWT extraction and collection is better between 250g and 350g in the actual test process. Wherein, the higher the water content of the biological sample, the less biomass is required, and vice versa. Based on the differences in the amount of biological sample and the density of biological species, in a preferred embodiment, the vacuum oven 10 employs a liner size (mm) of: 320 The method has the advantages that (length) multiplied by 320 (width) multiplied by 300 (height) can meet the requirement of accommodating a sufficient amount of biological samples (different biological types meet the capacity requirement) at one time, heating and sucking TFWT in the body of the biological samples, multiple test extractions are not needed, and the test period duration is reduced. When the device provided by the utility model is used for extracting and collecting free water tritium from biological sample tissues, the biological samples do not need to be chopped, and only clean biological samples are paved on the tray and then are placed in the sample chamber of the drying box, so that human resources and treatment time are further saved.
Referring to fig. 1, in the present utility model, the condensing unit further includes a low-temperature cooling liquid circulation pump 202, the low-temperature cooling liquid circulation pump 202 is provided with a cooling liquid inlet and a cooling liquid outlet, the condensing pipe 201 includes an inner pipe 2011 and an outer pipe (not labeled in the drawing), the inner pipe 2011 is sleeved in the outer pipe, the outer pipe is provided with a liquid inlet and a liquid outlet (the condensing pipe generally follows the principle of liquid inlet at the lower port and liquid outlet at the upper port), the liquid inlet is communicated with the cooling liquid outlet, and the liquid outlet is communicated with the cooling liquid inlet. The condenser 201 may be a serpentine condenser or a straight condenser or a bulb condenser, which are commonly used in the market.
In the utility model, the main function of the condensing component is to cool TFWT steam pumped out by a vacuum drying oven into liquid tritium water. In a specific embodiment, the volume of the cooling fluid in the low-temperature cooling fluid circulation pump 202 may be 4L, the temperature range is-10-normal temperature, and the flow rate is 15L/min. The low-temperature antifreezing cooling liquid can be a cooling liquid consisting of 30% of ethanol and 70% of deionized water by volume, and the condensation point of the cooling liquid is about-10 ℃, so that the functional effect of cooling liquid circulation cooling can be satisfied. The extraction and collection device in the prior art adopts liquid nitrogen as a cold trap, so that the use cost is high, and the risk that the air duct is blocked by the water vapor freezing ice column to cause the air duct or the connecting device to be flushed by high pressure exists. The utility model adopts the low-temperature cooling liquid circulating pump as a cooling device, is convenient for controlling the actual cooling temperature, and can ensure that TFWT steam is effectively condensed and can not be frozen into ice to influence the flow property of a pipeline. In operation, the temperature of the cryocoolant circulation pump 202 may be set at 2-6deg.C, e.g., 2deg.C, 4deg.C, 6deg.C, to ensure that TFWT vapor effectively condenses while not freezing to ice to affect the flow through the tubing. As an example, the cryo-coolant circulation pump 202 may be a model LC-LTC-5/10 cryo-coolant circulation pump manufactured by the company of instrument technology, inc.
In a preferred embodiment, the effect of condensing TFWT vapor into tritium water is further ensured by selecting a serpentine condenser tube and extending the length of the serpentine condenser tube and its layout in combination with the use of the cryogenic coolant circulation pump 202. A serpentine condenser pipe with the effective length of 600mm, the caliber of 24mm and the outer diameter of 40mm is selected, and the circulation resistance of TFWT steam is increased by obliquely arranging the condenser pipe 201 and prolonging the detention time of condensed tritium water in the condenser pipe, so that the condensation effect of the TFWT steam is improved. The angle of the condenser tube 201 to the horizontal may be about 45.
In the present utility model, the suction filter connector 30 may be a tee joint made of glass material, and may be further modified into a water vapor filter, where the inner cavity accommodates a filter element. The tritium water of the TFWT after cooling, which is led out after condensation through the inner pipe 2011 of the condensation pipe 202, also contains a small amount of water vapor and air components, and the tail end of the condensation pipe is connected with a water vapor filter, so that the collection efficiency of the TFWT can be further improved. The water vapor filter not only can filter tritium water flowing out of the condenser pipe 201, but also has the functions of liquefying vapor and fog, and discharges filtered air. The water vapor filter may be disposed in an inclined or upright arrangement so that filtered tritium water may flow under gravity from the bottom end interface 302 into the tritium water collector 40.
In a preferred embodiment, the selected vapor filter is configured with a vertical inlet and a horizontal outlet, with a drain port (i.e., bottom port 302) at the bottom. The water vapor filter adopts a vertical arrangement mode, filtered and collected TFWT tritium water can directly flow into the tritium water collector 40 from the bottom end interface 302, and filtered air is pumped out from the side wall interface 303. The sidewall interface 303 may be further designed to be disposed proximate the top end. As an example, the Filter connector may be a CEMS-Filter with a model GL-2T manufactured by su zhou Jing-huan Filter technology, and has a length of about 20cm and a diameter of about 6cm, the Filter connector housing is made of thickened high-quality transparent glass material, the highest withstand temperature reaches 180 ℃, the corrosion resistance is strong, the pore diameter of the matched Filter core can reach 0.1 μm, the Filter precision is high, the water vapor and mist in the gas can be effectively separated, and the Filter effect of the TFWT is ensured.
In the utility model, the vacuum pump 50 is sequentially connected with the suction filter connector 30, the condenser pipe 201 and the vacuum drying oven 10 through the connecting pipeline 60 (such as a connecting hose), so that a negative pressure environment can be provided for the interior of the pipeline, the circulation of TFWT steam, air, moisture and the like is promoted, and the collection rate of the TFWT is accelerated. In one embodiment, the vacuum pump 50 may be a rotary vane vacuum pump, and specific parameters of the vacuum filtration pump may be: the dimensions (length. Times. Width. Times. Height) were 300X 120X 235mm, the pumping rate was 120L/min, the limiting pressure was about 950kpa, the vacuum in the tube was reduced to 50mbar, and the operation was continued for 24 hours.
Based on the above structure and design, the present utility model may further include a dryer 70. One end of the dryer 70 is connected to the sidewall interface 303 of the connector body, and the other end is connected to the vacuum pump 50. Referring to fig. 1, the dryer 70 may be a straight tube with two ends communicating with each other, and a desiccant 701 is accommodated in the tube. The dryer 70 is preferably arranged upright, with the air exiting the suction connector 30 (sidewall interface 303) being fed from the bottom of the straight tube and being discharged from the top. The main function of the dryer 70 is to further dry the air exiting the suction connector 30 to reduce the corrosion of the vacuum pump 50 by the moisture in the air. In one embodiment, the drying tube 50 is formed of a straight tube having a length of about 20cm and a diameter of about 5cm and having two ends communicating with each other, the tube wall is formed of transparent glass, and SiO is contained in the tube 2 And (5) drying agent. In use, the color change (SiO) of the desiccant in the tube can be observed 2 The desiccant is normally blue in color and turns pink after being wet) to replace the desiccant. The wet and color-changed drying agent can be reused after being dried, and is convenient and practical.
The working flow of the biological sample tissue free water tritium extraction and collection device provided by the utility model can comprise the following steps:
(1) Pre-cooling of the condensing assembly: the circulation of the cooling liquid and the refrigerating button of the low-temperature cooling liquid circulation pump 202 are opened, and pre-refrigerating is carried out for 20 minutes, so that the temperature of the cooling liquid is reduced to about 4 ℃ (the acceptable error range is +/-2 ℃);
(2) Heating the biological sample: cleaning biological samples, spreading the biological samples in a tray, placing the biological samples in a sample chamber of a vacuum drying oven 10, closing a chamber door and a release valve 102 of the vacuum drying oven 10, opening a vacuum valve 101 and a heating switch, and setting the temperature of the sample chamber of the vacuum drying oven to be 105 ℃; vacuumizing the vacuum drying oven 10 through the vacuum pump 50, closing the vacuum valve 101 of the vacuum drying oven 10 when the vacuum degree in the vacuum drying oven 10 is 85kpa, and then closing the switch of the vacuum pump 50; the biological sample begins to be heated in the vacuum drying oven 10, releasing TFWT water vapor;
(3) Suction filtration TFWT vapor
Due to the evaporation of TFWT steam in the biological sample in the vacuum drying oven 10, the vacuum degree in the vacuum drying oven 10 is continuously reduced, when the vacuum degree in the vacuum drying oven 10 is reduced to 50kpa, the vacuum pump 50 and the vacuum valve 101 of the vacuum drying oven 10 are sequentially opened, the TFWT steam starts to be extracted, the steam is pumped into the condenser pipe 201 through the connecting pipe to be condensed into liquid tritium water, then flows in through the top end interface 301 of the suction filter connector 30, flows out into the tritium water collector 40 through the bottom end interface 302 and is collected into tritium water to be detected and analyzed later;
(4) Closing device
After a sufficient amount of tritium water is collected, a heating switch of the vacuum drying oven 10 is turned off, the vacuum pump 50 is utilized to continuously vacuumize for about 1 hour, then the vacuum valve 101 of the vacuum drying oven 10 is turned off, the air release valve 102 is turned on, then the low-temperature cooling liquid circulating pump 202 and the vacuum pump 50 are turned off in sequence, finally the tritium water collector is taken out, and the biological free water tritium is poured out.
The biological sample tissue free water tritium extraction and collection device (according to the layout shown in fig. 1, a serpentine condenser tube is adopted to be obliquely arranged, a water vapor filter and a dryer are added and are vertically arranged) is used for carrying out TFWT (thin film wt) collection and extraction tests on different types of organisms according to the method, and test results show that various organisms are laid in a sample chamber of a vacuum drying box after pretreatment, heated and pumped in the device for 2 hours, and continuously pumped for 1 hour after heating is stopped, enough tritium water can be collected (specific test data are shown in table 1), and the requirements of subsequent detection and analysis on the sample amount are met. The test result shows that the extraction efficiency of the device for biological TFWT is obviously higher than that of the current widely popular modes such as freeze dehydration, vacuum freeze drying, azeotropic distillation and the like, and the condensation collection efficiency of TFWT is better than or is at the same level with other treatment modes.
TABLE 1 TFWT extraction and collection test results provided by the present utility model
The biological tissue free tritium (TFWT) extraction and collection device provided by the utility model is safe, convenient and efficient, and the use and popularization of the device can effectively improve the detection/monitoring working efficiency of the biological tissue free tritium in radiation environment monitoring.
The above embodiments are only for illustrating the technical solution of the present utility model and not for limiting the same, and although the above embodiments specifically illustrate the present utility model, it should be understood by those skilled in the art that modifications and equivalents may be made to the specific embodiments of the present utility model without departing from the spirit and scope of the present utility model, and any modifications and equivalents are intended to be covered by the scope of the claims of the present utility model.
Claims (10)
1. A biological sample tissue free water tritium draws collection device which characterized in that: comprising the following steps:
the vacuum drying box is provided with a vacuum valve and a gas release valve;
a condensing assembly comprising a condensing tube having an inlet and an outlet, the inlet being connected to the vacuum valve;
the suction filter connector comprises a connector body, wherein a cavity is formed in the connector body in a hollow mode, interfaces are arranged at the top end, the bottom end and the side wall of the connector body, the interfaces are communicated with the cavity, and the interface at the top end of the connector body is connected with the outlet of the condenser pipe;
the tritium water collector is connected with the bottom end of the connector body;
and the vacuum pump is connected with an interface of the side wall of the connector body, so that the vacuum pump is sequentially connected with the suction filter connector, the condensing tube and the vacuum drying box.
2. The biological sample tissue free water tritium extraction and collection device of claim 1, wherein: the suction filter connector is a water vapor filter, and a filter element is also accommodated in the cavity.
3. The biological sample tissue free water tritium extraction and collection device of claim 2, wherein: the vacuum pump is characterized by further comprising a dryer, wherein one end of the dryer is connected with the interface of the side wall of the connector body, and the other end of the dryer is connected with the vacuum pump.
4. The biological sample tissue free water tritium extraction and collection device of claim 1, wherein: the condensing assembly further comprises a low-temperature cooling liquid circulating pump, the low-temperature cooling liquid circulating pump is provided with a cooling liquid inlet and a cooling liquid outlet, the condensing tube comprises an inner tube and an outer tube, the inner tube is sleeved in the outer tube, the outer tube is provided with a liquid inlet and a liquid outlet, the liquid inlet is communicated with the cooling liquid outlet, and the liquid outlet is communicated with the cooling liquid inlet.
5. The biological sample tissue free water tritium extraction and collection device of claim 1, 2 or 3, wherein: the condenser pipe is a snakelike condenser pipe, and the snakelike condenser pipe inclines to set up.
6. A biological sample tissue free water tritium extraction and collection device as claimed in claim 3, wherein: the suction filter connector and the dryer are vertically arranged, and the interface of the side wall of the connector body is close to the top end.
7. The biological sample tissue free water tritium extraction and collection device of claim 1, wherein: the vacuum drying oven is a constant-temperature electric heating vacuum drying oven, a plurality of sample chambers are arranged in the drying oven, and the temperature control range of the sample chambers is room temperature plus 10-200 ℃.
8. The biological sample tissue free water tritium extraction and collection device of claim 1, wherein: the vacuum pump is a rotary vane vacuum pump.
9. A biological sample tissue free water tritium extraction and collection device as claimed in claim 3, wherein: the dryer comprises a straight through pipe with two ends communicated, and a drying agent is contained in the pipe.
10. The device for extracting and collecting free water tritium from biological sample tissue according to claim 4, wherein: the use temperature of the low-temperature cooling liquid circulating pump is set to be 2-6 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320327992.3U CN219935373U (en) | 2023-02-27 | 2023-02-27 | Biological sample tissue free water tritium extraction and collection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320327992.3U CN219935373U (en) | 2023-02-27 | 2023-02-27 | Biological sample tissue free water tritium extraction and collection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219935373U true CN219935373U (en) | 2023-10-31 |
Family
ID=88497160
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320327992.3U Active CN219935373U (en) | 2023-02-27 | 2023-02-27 | Biological sample tissue free water tritium extraction and collection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219935373U (en) |
-
2023
- 2023-02-27 CN CN202320327992.3U patent/CN219935373U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11359860B2 (en) | Herb essential oil processing equipment | |
| CN106404459B (en) | The automation sampler of tritium in a kind of water | |
| CN219935373U (en) | Biological sample tissue free water tritium extraction and collection device | |
| CN106769389A (en) | One kind quickly catches up with sour instrument | |
| CN107462011A (en) | Air-drying box | |
| CN116465682A (en) | Method and device for extracting and collecting free water tritium from biological sample tissue | |
| CN109270226A (en) | A kind of the organic liquid volatilization and processing unit of controlled condition | |
| CN211635263U (en) | Self-heating water-saving intelligent rotary evaporator | |
| CN203971416U (en) | A kind of high-effect solvent concentration systems | |
| CN205920025U (en) | A full -automatic recycled asphalt retrieves appearance for trichloro ethylene extract | |
| CN110208042A (en) | It is a kind of biology sample in tissue free water tritium sampler | |
| CN205103070U (en) | Water -soluble attitude heavy metal sampling collection device in atmospheric particulates | |
| CN109883773A (en) | It is a kind of original position root system of plant volatile organic matter collect and measuring method | |
| CN206161390U (en) | Vacuum transfer device is collected to atmosphere steam | |
| CN212568131U (en) | Cigarette smoke trapping device of adjustable intake pipe length | |
| CN214472089U (en) | Simple exhaled air condensate collecting device | |
| CN213995348U (en) | Organic waste gas's absorption regenerating unit | |
| CN210921972U (en) | Vacuum drying oven with cold trap | |
| CN209132249U (en) | A kind of the organic liquid volatilization and processing unit of controlled condition | |
| CN208493299U (en) | A kind of refrigerating plant being pyrolyzed instrument | |
| CN203112792U (en) | Device for extracting plant essential oil | |
| CN109603187B (en) | Device and method for collecting aroma in Tieguanyin making process | |
| CN209052710U (en) | New Refrigerating anaerobic culture box | |
| CN110772822A (en) | Self-heating water-saving intelligent rotary evaporator | |
| CN112924248A (en) | Particulate matter collecting device and method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |