CN219637233U - Stem cell culture preparation device - Google Patents
Stem cell culture preparation device Download PDFInfo
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- CN219637233U CN219637233U CN202320688603.XU CN202320688603U CN219637233U CN 219637233 U CN219637233 U CN 219637233U CN 202320688603 U CN202320688603 U CN 202320688603U CN 219637233 U CN219637233 U CN 219637233U
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- cell culture
- stem cell
- preparation device
- culture
- placing
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 37
- 238000004113 cell culture Methods 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 238000010438 heat treatment Methods 0.000 claims abstract description 15
- 230000000844 anti-bacterial effect Effects 0.000 claims description 10
- 230000001954 sterilising effect Effects 0.000 claims description 7
- 238000003466 welding Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 abstract description 9
- 239000003570 air Substances 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 230000002070 germicidal effect Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 239000012080 ambient air Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000011982 device technology Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000001778 pluripotent stem cell Anatomy 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the technical field of cell culture, in particular to a stem cell culture preparation device, which comprises a constant temperature box, wherein a perspective box door is movably connected to the outside of the constant temperature box, sliding rails are inserted at two sides of the inner wall of the constant temperature box, pulleys are embedded in the sliding rails, a placing plate is connected to the outside of the pulleys in a sliding manner, air holes are formed in the placing plate, a limiting spring is fixedly connected to the upper surface of the placing plate, a first culture dish is inserted at one end of the limiting spring, the improved stem cell culture preparation device is provided with a humidifier and a heating plate, a dry and wet dual-purpose culture environment is realized, a plurality of stem cells are cultured at one time, culture conditions are convenient to control, culture efficiency is improved, the growth environment of the stem cells is observed in real time through a temperature detector and a humidity detector, meanwhile, the internal environment can be adjusted at any time through a fan and a sealing cover, and culture success rate is improved.
Description
Technical Field
The utility model relates to the technical field of cell culture, in particular to a stem cell culture preparation device.
Background
Stem cells are cells with self-replication function and multidirectional differentiation potential, can regenerate various cells, tissues or organs of a human body under specific conditions, are called universal cells in the medical field, have great significance in basic research and transformation medicine application, and have wide application prospects in the fields of regenerative medicine, disease models, medicine screening, precise medicine and the like, but due to the fact that the conventional stem cells exist in insufficient quantity, the stem cell research is developing in the field of inducing the pluripotent stem cells, the problem that the stem cells are used as seed cells is attempted to be solved, a stem cell culture device is developed in China, a large number of stem cells are cultured, and the existing stem cell culture device technology is not mature and any of the stem cell culture device needs to be solved.
The prior patent (publication number: CN 214781903U) discloses a stem cell culture device, relates to the technical field of stem cell culture, and comprises: the device comprises a box body, a lifting device, a culture table, a culture dish, a transfusion device and a liquid suction device, wherein one end of the lifting device is fixedly connected to the inner bottom surface of the box body, the culture table is arranged at the other end of the lifting device, the culture dish is arranged on the culture table, and the transfusion device and the liquid suction device are all arranged at the top of the box body. Through the arrangement of the device, the cell culture solution can be replaced under the condition that the box door is not opened, so that the possibility of pollution in the incubator body is reduced.
The inventors found that the following problems exist in the prior art in the process of implementing the present utility model: 1. the culture environment is monotonous, a plurality of stem cells cannot be simultaneously cultured at one time, and the culture efficiency is low; 2. the external growth environment of the stem cells cannot be observed in real time, timely adjustment and modification cannot be performed, and finally culture failure can be caused.
Disclosure of Invention
The utility model aims to provide a stem cell culture preparation device for solving the problems in the background art. In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a stem cell culture preparation facilities, includes the thermostated container, the outside swing joint of thermostated container has perspective chamber door, peg graft there is the slide rail in the inner wall both sides of thermostated container, the inside of slide rail is embedded to have the pulley, the outside sliding connection of pulley has place the board, the bleeder vent has been seted up to the inside of placing the board, the last fixed surface of placing the board is connected with spacing spring, the one end grafting of spacing spring has a culture dish, the first bactericidal lamp of top fixedly connected with of culture dish, one side joint of culture dish has temperature detector, the below welding of placing the board has the hot plate, the both ends joint of hot plate has the fan, sealed lid has been cup jointed to the top of fan, the below fixedly connected with heat insulating board of hot plate, the below swing joint of heat insulating board has the second bactericidal lamp, the below swing joint of second bactericidal lamp has the culture dish No. two, the top of thermostated container is embedded to have the filter, the top threaded connection of filter has the piston, the inside of inner wall bottom of thermostated container has the humidifier, the humidity detector of humidifier, the universal wheel has the universal wheel of peg graft.
Further preferably, the spacing spring and the placing plate are arranged in parallel.
Further preferably, the heating plate and the placing plate are arranged in parallel.
Further preferably, two placing plates are provided, and the two placing plates are respectively located below the first sterilizing lamp and below the second sterilizing lamp.
Further preferably, two fans are provided, and the two fans are respectively positioned at two ends of the heating plate.
Further preferably, the first culture dish and the placing plate are arranged in a vertical structure.
Compared with the prior art, the utility model has the beneficial effects that:
according to the utility model, through the arrangement of the humidifier and the heating plate, a dry-wet dual-purpose culture environment is realized, a plurality of stem cells are cultured at one time, the culture efficiency is high, the culture condition is convenient to control, and the culture efficiency is improved.
According to the utility model, the growth environment of stem cells is observed in real time through the temperature detector and the humidity detector, meanwhile, the internal environment can be adjusted at any time through the fan and the sealing cover, the sealing cover is arranged above the fan, when the heating plate is heated, the sealing cover and the fan are opened to form hot air flow circulation, the humidity and the temperature are adjusted at any time, and the success rate of culture is improved.
Drawings
FIG. 1 is a schematic diagram of the front internal structure of the present utility model;
FIG. 2 is a schematic side view of the internal structure of the present utility model;
FIG. 3 is a schematic top view of the internal structure of the present utility model;
FIG. 4 is a schematic view of the internal structure of the fan of the present utility model.
In the figure: 1. a constant temperature box; 2. perspective box door; 3. a slide rail; 4. a pulley; 5. placing a plate; 6. ventilation holes; 7. a limit spring; 8. a first culture dish; 9. a first germicidal lamp; 10. a temperature detector; 11. a heating plate; 12. a blower; 13. sealing cover; 14. a heat insulating plate; 15. a second germicidal lamp; 16. a second culture dish; 17. a filter; 18. a piston; 19. a humidifier; 20. a humidity detector; 21. a universal wheel; 22. a brake pad.
Description of the embodiments
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which are obtained by a worker of ordinary skill in the art without creative efforts, are within the protection scope of the present utility model based on the embodiments of the present utility model.
Referring to fig. 1 to 4, the present utility model provides a technical solution: the utility model provides a stem cell culture preparation facilities, including incubator 1, the outside swing joint of incubator 1 has perspective chamber door 2, the inner wall both sides grafting of incubator 1 has slide rail 3, the inside of slide rail 3 is embedded to have pulley 4, the outside sliding connection of pulley 4 has place board 5, bleeder vent 6 has been seted up to the inside of placing board 5, the last fixed surface of placing board 5 is connected with spacing spring 7, the one end grafting of spacing spring 7 has culture dish 8, the top fixedly connected with first bactericidal lamp 9 of culture dish 8, one side joint of culture dish 8 has temperature detector 10, the below welding of placing board 5 has hot plate 11, the both ends joint of hot plate 11 has fan 12, sealed lid 13 has been cup jointed to fan 12's top, the below fixedly connected with heat insulating board 14 of hot plate 11, the below swing joint of heat insulating board 14 has second bactericidal lamp 15, the below swing joint of second bactericidal lamp 15 has culture dish 16 No. two, the top of incubator 1 is embedded to have filter 17, the top threaded connection of filter 17 has piston 18, the inside of inner wall bottom of incubator 1 has humidifier 19, the universal wheel 21 of humidifier 19 has the universal wheel 21 of humidity detector, the universal wheel 21 is connected with the outside of the universal wheel 21.
In this embodiment, as shown in fig. 1, a parallel structure is set between the spacing spring 7 and the placement plate 5, and it needs to be described that, when the first culture dish 8 is clamped into the upper surface of the placement plate 5, the horizontal line of the spacing spring 7 is parallel to the placement plate 5, the top of the spacing spring 7 is contracted inwards, and the spacing spring 7 is compressed, so that the first culture dish 8 is fixed more tightly, the spacing spring 7 is reset when being taken, and no blocking is formed, and the embodiment is more convenient.
In this embodiment, as shown in fig. 1, a parallel structure is formed between the heating plate 11 and the placing plate 5, and it should be noted that, when the heating plate 11 heats, the upper portion of the placing plate 5 can be uniformly heated, and the air holes 6 are formed in the placing plate 5, so that the effect of rapid heating and drying can be achieved through the air holes 6.
In this embodiment, as shown in fig. 1 and 3, two placement plates 5 are provided, and the two placement plates 5 are respectively located below the first germicidal lamp 9 and below the second germicidal lamp 15, and it should be noted that the two placement plates 5 are respectively located in different environments, and the upper side of the placement plates respectively prevents the first culture dish 8 and the second culture dish 16, so as to realize simultaneous culture of a plurality of stem cells, and improve the culture efficiency of the stem cells.
In this embodiment, as shown in fig. 1 and fig. 4, two fans 12 are provided, and the two fans 12 are respectively located at two ends of the heating plate 11, it should be noted that, a sealing cover 13 is provided above the fans 12, when the heating plate 11 heats, the culture environment of the second culture dish 16 will not be affected, if the ambient air temperature of the second culture dish 16 needs to be increased, the sealing cover 13 and the fans 12 are opened to form a hot air flow circulation, and the hot air flow circulation is adjusted at any time, thereby improving the success probability of stem cell culture.
In this embodiment, as shown in fig. 1, a vertical structure is set between the first culture dish 8 and the placing plate 5, it needs to be noted that a first sterilizing lamp 9 is arranged above the first culture dish 8, a sliding rail 3 is arranged below the first culture dish 8, the first culture dish 8 is always kept vertical to the placing plate 5 through the sliding rail 3, stem cells are always in a stable state, and survival rate is improved.
The application method and the advantages of the utility model are as follows: the stem cell culture preparation device has the following working procedures when in use:
as shown in fig. 1, fig. 2, fig. 3 and fig. 4, firstly we open the perspective box door 2, put the first culture dish 8 and the second culture dish 16 with stem cells on the limit spring 7 of the placing plate 5, compress the first culture dish 8 and the second culture dish 16 by the spring, close the sealing cover 13, turn on the first sterilizing lamp 9 and the second sterilizing lamp 15, simultaneously turn on the humidifier 19 and the heating plate 11, observe the values of the temperature detector 10 and the humidity detector 20, wait until the required number is the accurate number suitable for the survival of the stem cells, close the humidifier 19 and the heating plate 11, observe the culture environment of the first culture dish 8 in real time through the temperature detector 10, observe the culture environment of the second culture dish 16 in real time through the humidity detector 20, such as the too high temperature opens the piston 18, avoid pollution while reducing the temperature, and if the second culture dish 16 needs to be heated, turn on the fan 12 and the sealing cover 13, the hot air flow above the fan circulates to increase the temperature, and reduce the humidity.
The foregoing has shown and described the basic principles, principal features and advantages of the utility model. It will be understood by those skilled in the art that the present utility model is not limited to the above-described embodiments, and that the above-described embodiments and descriptions are only preferred embodiments of the present utility model, and are not intended to limit the utility model, and that various changes and modifications may be made therein without departing from the spirit and scope of the utility model as claimed. The scope of the utility model is defined by the appended claims and equivalents thereof.
Claims (6)
1. The stem cell culture preparation device comprises an incubator (1), and is characterized in that: the utility model discloses a refrigerator, including incubator (1), slide rail (3) are pegged graft to the outside swing joint of incubator (1), slide rail (3) are pegged graft to the inner wall both sides of incubator (1), the inside of slide rail (3) is embedded to have pulley (4), the outside sliding connection of pulley (4) has place board (5), bleeder vent (6) have been seted up to the inside of placing board (5), the upper surface fixedly connected with spacing spring (7) of placing board (5), peg graft and have culture dish (8) No. one, the top fixedly connected with first bactericidal lamp (9) of culture dish (8) No. one side joint of culture dish (8) has temperature detector (10), the below welding of placing board (5) has hot plate (11), the both ends joint of hot plate (11) has fan (12), sealed lid (13) have been cup jointed to the top of fan (12), the below fixedly connected with (14) of hot plate (11), the below swing joint of heat insulating plate (14) has first bactericidal lamp (15), the top of second bactericidal lamp (15) has screw thread connection (17) on second bactericidal lamp (17), the intelligent constant temperature cabinet is characterized in that a humidifier (19) is embedded in the bottom of the inner wall of the constant temperature cabinet (1), a humidity detector (20) is inserted above the humidifier (19), a universal wheel (21) is fixedly connected to the bottom of the constant temperature cabinet (1), and a brake block (22) is inserted outside the universal wheel (21).
2. The stem cell culture preparation device according to claim 1, wherein: the spacing spring (7) and the placing plate (5) are arranged into a parallel structure.
3. The stem cell culture preparation device according to claim 1, wherein: the heating plate (11) and the placing plate (5) are arranged in parallel.
4. The stem cell culture preparation device according to claim 1, wherein: the two placing plates (5) are arranged in total, and the two placing plates (5) are respectively positioned below the first sterilizing lamp (9) and below the second sterilizing lamp (15).
5. The stem cell culture preparation device according to claim 1, wherein: the two fans (12) are arranged in total, and the two fans (12) are respectively positioned at two ends of the heating plate (11).
6. The stem cell culture preparation device according to claim 1, wherein: the first culture dish (8) and the placing plate (5) are arranged in a vertical structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320688603.XU CN219637233U (en) | 2023-03-31 | 2023-03-31 | Stem cell culture preparation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320688603.XU CN219637233U (en) | 2023-03-31 | 2023-03-31 | Stem cell culture preparation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219637233U true CN219637233U (en) | 2023-09-05 |
Family
ID=87819032
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320688603.XU Active CN219637233U (en) | 2023-03-31 | 2023-03-31 | Stem cell culture preparation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219637233U (en) |
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2023
- 2023-03-31 CN CN202320688603.XU patent/CN219637233U/en active Active
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