CN219024357U - Sample adding structure of fluorescence quantitative pcr instrument - Google Patents
Sample adding structure of fluorescence quantitative pcr instrument Download PDFInfo
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- CN219024357U CN219024357U CN202223478139.6U CN202223478139U CN219024357U CN 219024357 U CN219024357 U CN 219024357U CN 202223478139 U CN202223478139 U CN 202223478139U CN 219024357 U CN219024357 U CN 219024357U
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- 238000003753 real-time PCR Methods 0.000 title claims abstract description 13
- 238000002347 injection Methods 0.000 claims description 31
- 239000007924 injection Substances 0.000 claims description 31
- 238000007789 sealing Methods 0.000 claims description 5
- 239000000654 additive Substances 0.000 abstract description 2
- 230000000996 additive effect Effects 0.000 abstract description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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Abstract
The utility model discloses a sample adding structure of a fluorescence quantitative pcr instrument, which comprises a base, wherein the base is fixedly connected with a top plate through two supporting rods, the lower end of the top plate is fixedly connected with a guide rod, the lower end of the guide rod is fixedly connected to the base, the base is rotatably connected with a lifting screw, the upper end of the top plate is fixedly connected with a servo motor, an output shaft of the servo motor is fixedly connected with the lifting screw, a sample adding plate is connected to the lifting screw through threads, a plurality of test tube grooves are formed in the upper end of the sample adding plate, and the lower end of the top plate is fixedly connected with a sample adding box through a connecting rod. According to the utility model, by arranging the servo oil cylinder, the piston plate can be pulled to move up and down through the servo oil cylinder, so that samples with the same dosage can be added to all test tubes on the sample adding plate at one time, the same additive dosage of all test tubes is ensured, the operation efficiency is obviously improved, and meanwhile, the sample pollution caused by manual operation is avoided.
Description
Technical Field
The utility model relates to the technical field of pcr instruments, in particular to a sample adding structure of a fluorescence quantitative pcr instrument.
Background
The fluorescent quantitative PCR instrument is one kind of analytical instrument for chemical, biological, agricultural and forestry fields and is used mainly in quantitative analysis of unknown template via standard curve.
When a sample is added to a PCR instrument, it is necessary to add the sample to a plurality of test tubes to obtain sufficient experimental data, and then to put the test tube containing the sample into PCR for analysis. At present, samples are generally added to a plurality of test tubes manually, so that the dosage of each test tube is difficult to ensure to be the same, the analysis result of subsequent experiments is influenced, and meanwhile, the operation of adding samples one by one is performed, so that the sample adding efficiency is lower.
Disclosure of Invention
The utility model aims to solve the defects in the prior art, and provides a sample adding structure of a fluorescence quantitative pcr instrument.
In order to achieve the above purpose, the present utility model adopts the following technical scheme:
the utility model provides a fluorescence ration pcr appearance's application of sample structure, includes the base, the base passes through two bracing piece fixedly connected with roof, roof lower extreme fixedly connected with guide bar, just the lower extreme fixedly connected with of guide bar is on the base, rotate on the base and be connected with lifting screw, roof upper end fixedly connected with servo motor, servo motor's output shaft and lifting screw fixed connection, threaded connection has the application of sample board on the lifting screw, a plurality of test tube grooves have been seted up to application of sample board upper end, the roof lower extreme passes through connecting rod fixedly connected with application of sample box, sealing sliding connection has the piston board in the application of sample box, the through-hole has been seted up to the piston board upper end, just the application of sample box intercommunication has the application of sample pipe, all install the check valve in application of sample pipe and the through-hole, application of sample box lower extreme intercommunication has a plurality of injection pipes.
Preferably, an air bag is fixedly connected to the bottom in the test tube groove, a groove is formed in the base, and an air injection device for injecting air into the air bag is arranged in the groove.
Preferably, the gas injection device comprises a plunger, a spring and a plurality of gas guide pipes, wherein a gas injection groove is formed in the inner wall of the groove, the plunger is connected in the gas injection groove in a sealing sliding mode, the plunger is connected to the inner wall of the gas injection groove through the spring, the gas guide pipes are used for communicating corresponding air bags with the gas injection groove, and a moving mechanism for enabling the plunger to move is arranged in the groove.
Preferably, the moving mechanism comprises a one-way bearing, a rack and an incomplete gear, wherein the incomplete gear is connected with the lifting screw rod through the one-way bearing, and the rack is fixedly connected with the pushing plug.
Preferably, a servo oil cylinder is fixedly arranged at the lower end of the top plate, and the telescopic end of the servo oil cylinder is fixedly connected with the piston plate.
Preferably, one end of the spring is fixedly connected with the plunger, and the other end of the spring is fixedly connected to the inner wall of the gas injection groove.
The utility model has the following beneficial effects:
1. through setting up servo cylinder, accessible servo cylinder pulling piston board reciprocates, can once only add the sample of the same dose to each test tube on the sample plate, guarantee that each test tube additive dose is the same still obviously improved operating efficiency, still avoided manual operation to pollute the sample simultaneously.
2. Through setting up gasbag and gas injection device, can be when the sample board moves down, constantly shake each test tube, promote the sample misce bene in the test tube.
Drawings
FIG. 1 is a schematic structural diagram of a sample adding structure of a fluorescence quantitative pcr instrument;
FIG. 2 is an enlarged schematic view of the structure at A in FIG. 1;
FIG. 3 is a schematic perspective view of the sample plate according to the present utility model.
In the figure: 1 a base, 2 support rods, 3 top plates, 4 guide rods, 5 lifting screws, 6 servo motors, 7 servo cylinders, 8 connecting rods, 9 sample adding boxes, 10 piston plates, 11 sample adding pipes, 12 through holes, 13 injection pipes, 14 sample adding plates, 15 sample pipe grooves, 16 air bags, 17 test pipes, 18 one-way bearings, 19 incomplete gears, 20 racks, 21 pushing plugs, 22 springs, 23 air ducts, 24 air injection grooves and 25 grooves.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments.
In the description of the present utility model, it should be understood that the terms "upper," "lower," "front," "rear," "left," "right," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the present utility model and to simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model.
Referring to fig. 1-3, a sample loading structure of a fluorescence quantitative pcr instrument comprises a base 1, the base 1 is fixedly connected with a top plate 3 through two support rods 2, the lower end of the top plate 3 is fixedly connected with a guide rod 4, the lower end of the guide rod 4 is fixedly connected to the base 1, a lifting screw 5 is rotatably connected to the base 1, the upper end of the top plate 3 is fixedly connected with a servo motor 6, an output shaft of the servo motor 6 is fixedly connected with the lifting screw 5, a sample loading plate 14 is in threaded connection with the lifting screw 5, a plurality of test tube slots 15 are formed in the upper end of the sample loading plate 14, the lower end of the top plate 3 is fixedly connected with a sample loading box 9 through a connecting rod 8, a piston plate 10 is in sealing sliding connection with the sample loading box 9, a servo cylinder 7 is fixedly arranged at the lower end of the top plate 3, and a telescopic end of the servo cylinder 7 is fixedly connected with the piston plate 10.
The upper end of the piston plate 10 is provided with a through hole 12, the sample adding box 9 is communicated with a sample adding pipe 11, check valves are arranged in the sample adding pipe 11 and the through hole 12, and the lower end of the sample adding box 9 is communicated with a plurality of injection pipes 13. It should be noted that the sample adding tube 11 may be directly connected to the container containing the sample, and the check valve in the sample adding tube 11 allows the sample to flow only from the sample adding tube 11 to the sample adding cartridge 9, and the check valve in the through hole 12 allows the sample to flow only from above the piston plate 10 to below.
Further, when the sample flows from above to below the piston plate 10, if the piston plate 10 is not pressed downward, the sample below it does not naturally flow out under the air pressure balance, so that no dripping occurs.
The air bag 16 is fixedly connected to the bottom in the test tube groove 15, the base 1 is provided with a groove 25, and an air injection device for injecting air into the air bag 16 is arranged in the groove 25. The gas injection device comprises a push plug 21, a spring 22 and a plurality of gas guide pipes 23, wherein a gas injection groove 24 is formed in the inner wall of a groove 25, the push plug 21 is connected in the gas injection groove 24 in a sealing sliding manner, the push plug 21 is connected to the inner wall of the gas injection groove 24 through the spring 22, one end of the spring 22 is fixedly connected with the push plug 21, and the other end of the spring 22 is fixedly connected to the inner wall of the gas injection groove 24.
Each air duct 23 communicates the corresponding air bag 16 with the air injection groove 24, and a moving mechanism for moving the plunger 21 is installed in the groove 25.
The moving mechanism comprises a one-way bearing 18, a rack 20 and an incomplete gear 19, wherein the incomplete gear 19 is connected with the lifting screw 5 through the one-way bearing 18, and the rack 20 is fixedly connected with the plunger 21. It should be noted that, as shown in fig. 2, the incomplete gear 19 is matched with the rack 20, when the incomplete gear 19 continuously rotates, the incomplete gear 19 will be periodically meshed with the rack 20, when the incomplete gear 19 is meshed with the rack 20, the rack 20 and the plunger 21 can be pulled to move in a certain direction, and when the incomplete gear 19 is separated from the rack 20, the spring 22 can pull the plunger 21 to reset. This moves the plunger 21 back and forth left and right, thereby evacuating or inflating the balloon 16 through the air duct 23, and finally expanding and contracting the balloon 16 to push the cuvette 17.
In addition, the one-way bearing 18 does not drive the incomplete gear 19 to rotate when the lifting screw 5 rotates forward, and can transmit the torque of the lifting screw 5 to the incomplete gear 19 to drive the incomplete gear 19 to rotate when the lifting screw 5 rotates reversely.
When the device is used, after the test tube 17 is placed in the test tube groove 15, the servo motor 6 can be started to drive the lifting screw 5 to rotate forward, at the moment, the sample adding plate 14 can be lifted, and each injection tube 13 can enter the test tube 17, so that samples can be conveniently injected and dripping can be prevented.
Subsequently, the servo cylinder 7 is operated to drive the piston plate 10 to move downwards, so that the airtight space above the piston plate 10 is increased, negative pressure can be generated, and a certain amount of sample can be sucked into the sample loading box 9 through the sample loading tube 11.
Then the servo cylinder 7 is controlled to pull the piston plate 10 to move upwards, so that samples above the piston plate 10 can be forced to enter below the piston plate 10 through the through holes 12, the piston plate 10 is moved downwards again, and the samples below the piston plate 10 can be uniformly extruded into the test tubes 17 through the injection tubes 13, so that the uniformity of the added sample metering of the test tubes 17 can be ensured, and meanwhile, the samples can be added into a plurality of test tubes 17 at one time, and the efficiency is high.
In addition, after the sample is added, the servo motor 6 can be controlled to drive the lifting screw 5 to rotate reversely, at the moment, the lifting screw 5 drives the incomplete gear 19 to rotate circularly through the one-way bearing 18, and the incomplete gear 19 can be meshed with the rack 20 periodically when continuously rotating, so that the plunger 21 moves left and right under the action of the spring 22, and when the plunger 21 moves left, air is pumped from the air bag 16, so that the air bag 16 contracts; when the plunger 21 moves right, the air in the air injection groove 24 is input into the air bag 16 along the air duct 23 to expand, so that the air bag 16 is continuously expanded and contracted in the downward movement process of the sample adding plate 14, and the test tube 17 in the test tube groove 15 can be continuously and slightly pushed to promote uniform mixing of samples in the test tube 17.
The foregoing is only a preferred embodiment of the present utility model, but the scope of the present utility model is not limited thereto, and any person skilled in the art, who is within the scope of the present utility model, should make equivalent substitutions or modifications according to the technical scheme of the present utility model and the inventive concept thereof, and should be covered by the scope of the present utility model.
Claims (6)
1. The utility model provides a fluorescence ration pcr appearance's application of sample structure, includes base (1), its characterized in that, base (1) is through two bracing pieces (2) fixedly connected with roof (3), roof (3) lower extreme fixedly connected with guide bar (4), just the lower extreme fixedly connected with of guide bar (4) is on base (1), rotate on base (1) and be connected with lift screw (5), roof (3) upper end fixedly connected with servo motor (6), the output shaft and lift screw (5) fixed connection of servo motor (6), threaded connection has application of sample board (14) on lift screw (5), a plurality of test tube grooves (15) have been seted up to application of sample board (14) upper end, roof (3) lower extreme is through connecting rod (8) fixedly connected with application of sample box (9), sealed sliding connection has piston plate (10) in application of sample box (9), through-hole (12) have been seted up to piston plate (10) upper end, just application of sample box (9) intercommunication has application of sample board (11), application of sample board (11) and injection valve (13) have in the injection case (13) all to connect.
2. The sample adding structure of the fluorescence quantitative pcr instrument according to claim 1, wherein an air bag (16) is fixedly connected to the bottom in the test tube groove (15), a groove (25) is formed in the base (1), and an air injection device for injecting air into the air bag (16) is arranged in the groove (25).
3. The sample adding structure of the fluorescence quantitative pcr instrument according to claim 2, wherein the gas injection device comprises a plunger (21), a spring (22) and a plurality of gas guide pipes (23), a gas injection groove (24) is formed in the inner wall of the groove (25), the plunger (21) is connected in the gas injection groove (24) in a sealing sliding manner, the plunger (21) is connected to the inner wall of the gas injection groove (24) through the spring (22), each gas guide pipe (23) communicates a corresponding gas bag (16) with the gas injection groove (24), and a moving mechanism for moving the plunger (21) is installed in the groove (25).
4. The sample adding structure of the fluorescence quantitative pcr instrument according to claim 3, wherein the moving mechanism comprises a one-way bearing (18), a rack (20) and an incomplete gear (19), the incomplete gear (19) is connected with the lifting screw (5) through the one-way bearing (18), and the rack (20) is fixedly connected with the pushing plug (21).
5. The sample adding structure of the fluorescence quantitative pcr instrument according to claim 1, wherein a servo oil cylinder (7) is fixedly arranged at the lower end of the top plate (3), and a telescopic end of the servo oil cylinder (7) is fixedly connected with the piston plate (10).
6. A sample loading structure of a fluorescence quantitative pcr instrument according to claim 3, wherein one end of the spring (22) is fixedly connected with the plunger (21), and the other end of the spring (22) is fixedly connected to the inner wall of the gas injection groove (24).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223478139.6U CN219024357U (en) | 2022-12-26 | 2022-12-26 | Sample adding structure of fluorescence quantitative pcr instrument |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223478139.6U CN219024357U (en) | 2022-12-26 | 2022-12-26 | Sample adding structure of fluorescence quantitative pcr instrument |
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| CN219024357U true CN219024357U (en) | 2023-05-16 |
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| CN202223478139.6U Active CN219024357U (en) | 2022-12-26 | 2022-12-26 | Sample adding structure of fluorescence quantitative pcr instrument |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115990529A (en) * | 2022-12-26 | 2023-04-21 | 上海大学温州研究院 | A sample loading structure of a fluorescent quantitative PCR instrument |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115990529A (en) * | 2022-12-26 | 2023-04-21 | 上海大学温州研究院 | A sample loading structure of a fluorescent quantitative PCR instrument |
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