CN219024109U - Microfluidic device - Google Patents

Abstract

The utility model relates to the technical field of microfluid control, in particular to a microfluidic device, which comprises a shell, wherein a liquid pushing mechanism is arranged in the shell, a microfluidic liquid phase mixing mechanism is communicated above the liquid pushing mechanism, a liquid receiving mechanism is communicated below the microfluidic liquid phase mixing mechanism, a touch screen interaction control mechanism is arranged at the upper part of the shell, and the touch screen interaction control mechanism is used for controlling and connecting the liquid pushing mechanism, the microfluidic liquid phase mixing mechanism and the liquid receiving mechanism; the utility model has simple structure, good large-screen touch interaction experience, convenient operation, high automation degree, stable preparation, high accuracy and good compatibility, can automatically control the liquid inlet amount and liquid inlet speed of raw material liquid, realize the accurate and rapid mixing of two-phase liquid with different metering and different mixing speeds, and accurately control the liquid inlet amount and liquid inlet speed of the two-channel raw liquid into the micro-fluid liquid phase mixing mechanism through automation, thereby achieving the aim of accurately controlling the proportion of mixing two groups of liquid phases.

Description

A microfluidic device

technical field

The utility model relates to the technical field of microfluid control, in particular to a microfluid control device.

Background technique

Microfluidics is a new research field based on the intersection of physics, chemistry, biology, fluid dynamics, microelectronics and material science. Microfluidics is considered to be a system science and technology capable of handling or manipulating small volumes (10 ^-9 ~ 10 ^-18 L) of fluids using channels of tens to hundreds of microns in size. In recent years, as microfluidic devices and technologies have been successfully used in the development and production of mRNA COVID-19 vaccines based on lipid nanoparticles (LNP), more and more different mRNA vaccines, such as preventing malaria, Vaccines such as human immunodeficiency virus, influenza and cancer are all using this research method for product research. As the key means of preparing nucleic acid carrier LNP, microfluidic equipment and technology have received unprecedented attention from various fields, especially the pharmaceutical industry.

In the preparation of lipid nanoparticles LNP, microfluidic devices and technologies present many advantages, such as relatively simple and fast operation, quick optimization of LNP preparation conditions, mild conditions, and easy production scale-up. These advantages have not only prompted LNPs prepared with microfluidic devices and techniques to be used as RNA delivery platforms, but also enabled laboratory-level use to be scaled up to actual product production applications. Currently, LNP-based RNA, DNA, ribonucleoprotein, drug and other nanoparticle delivery platforms are being researched and developed using microfluidic devices and technologies. In the future, microfluidic devices and techniques are likely to be used as industry standard means for LNP preparation. Nucleic acid/gene therapy is an emerging field with broad application prospects and value. As the most advantageous equipment and technology for preparing key delivery vectors, it will be a crucial part of this field.

At present, the commercialized microfluidic devices on the market mainly include NanoAssemblr and ILiNP products. The domestic market is mainly based on this kind of imported equipment. The chips used in the equipment can only be used once due to the program setting, which makes the use of microfluidics quite expensive. In addition, the current microfluidic devices (such as NanoAssemblr products) used for small-dose research and development still have the following main shortcomings: the maximum volume of single mixing is 12ml, which cannot meet the requirements for large-volume sample preparation; the maximum mixing speed is 16ml/min, It cannot meet the requirements of high-speed mixing of liquids; the types of compatible syringes are limited, and only 1ml syringes can be matched. However, domestic self-developed automated microfluidic equipment that can replace imported equipment is still very limited. Therefore, a microfluidic mechanism that can achieve different meter types, different mixing speeds, high compatibility, and precise and rapid mixing of two-phase liquids has been developed. has great significance.

Utility model content

The technical problem to be solved by the utility model is to provide a simple structure, good interactive experience of large-screen touch control, convenient operation, high degree of automation, stable preparation, high precision, automatic and precise control of the liquid intake and liquid intake of the raw material liquid. A microfluidic device that achieves precise and rapid mixing of two-phase liquids of different meter types and different mixing speeds.

In order to solve the above-mentioned technical problems, the technical proposal of the present utility model is: a microfluidic device, including a casing, a pushing liquid mechanism is installed inside the casing, and a microfluidic liquid phase is connected above the pushing liquid mechanism. A mixing mechanism, a liquid contacting mechanism is connected under the microfluidic liquid phase mixing mechanism, a touch screen interactive control mechanism is installed on the upper part of the housing, and the touch screen interactive control mechanism controls and connects the pushing liquid mechanism, the The microfluidic liquid phase mixing mechanism and the liquid contact mechanism.

As a preferred technical solution, the pushing liquid mechanism includes two pushing liquid mounting seats installed at the inner bottom of the housing, each of the pushing liquid mounting seats is respectively vertically installed with a slide table screw, and each of the sliding table screws Pushing liquid faders are respectively installed on the screw rods of the platform, and initial position detection sensors are installed on the pushing liquid mounting base.

As a preferred technical solution, the pushing liquid clipper includes a clipper body installed on the screw rod of the slide table, the upper part of the clipper body is hinged with a clipper contact plate through a clipper contact plate bearing, and the clipper The lower part of the main body is fixedly installed with a clip support structure, and a trigger sensor is installed on the clip support structure, and the lower surface of the clip contact plate is in elastic contact with the trigger sensor.

As a preferred technical solution, the liquid contact mechanism includes a mixed liquid receiving device support body fixedly installed in the upper part of the housing, a semicircular slot is provided on the bottom surface of the mixed liquid receiving device support body, and the semicircular slot A semi-circular rotating liquid receiving structure is installed in the circular slot, one side of the rotating liquid collecting structure is provided with a rotating liquid collecting mechanism gear, and the bottom surface of the support body of the mixed liquid receiving device is also installed with the The driving gear of the rotating liquid collecting mechanism meshed with the gear of the rotating liquid collecting mechanism.

As a preferred technical solution, two collection test tube clamp structures are arranged in the middle of the support body of the mixed liquid receiving device, and the liquid-contacting centrifuge tube and the sample collection centrifuge tube are respectively installed on the collection test tube clamp structure.

As a preferred technical solution, the microfluidic liquid phase mixing mechanism includes a chip installed on the top of the support of the mixed liquid receiving device, a microfluidic mixing channel is provided inside the chip, and one end of the microfluidic mixing channel is provided with Two liquid inlets, the other end of the microfluidic mixing channel is provided with a liquid outlet, and the middle position of the rotating liquid receiving structure is provided with two syringe placement connection holes corresponding to the liquid inlet, Liquid inlet syringes are respectively installed in the connecting holes of the syringes, and the liquid outlets are used in conjunction with the liquid contact centrifuge tube and the sample collection centrifuge tube.

As a preferred technical solution, the chip is a Y-shaped structure chip or a T-shaped structure chip.

As a preferred technical solution, an opening is opened on the front of the housing, and a door is installed on the opening.

As a preferred technical solution, the touch screen of the touch screen interactive control mechanism forms an angle of 75° with the horizontal plane.

Due to the adoption of the above technical solution, a microfluidic device includes a housing, a liquid pushing mechanism is installed inside the housing, a microfluidic liquid phase mixing mechanism is connected above the liquid pushing mechanism, and a microfluidic liquid phase mixing mechanism is connected below the microfluidic liquid phase mixing mechanism. Connected with a liquid contact mechanism, the upper part of the housing is equipped with a touch screen interactive control mechanism, which controls and connects the push liquid mechanism, the micro-flow liquid phase mixing mechanism and the liquid contact mechanism; the beneficial effects of the utility model are:

(1) The equipment has a simple structure, a high degree of automation, a friendly interactive solution, and high preparation accuracy. The utility model achieves precise control of the mixing ratio of two groups of liquid phases through automatic and precise control of the feed volume and speed of two channel raw liquids entering the micro-fluid liquid phase mixing mechanism.

(2) Touch screen interactive control mechanism, including touch screen, micro-control chip, various sensor components, etc., to form a complete system. The UI interface is beautiful, intuitive, and easy to operate, and the feedback of the working status and process is intuitive and clear.

(3) The liquid pushing mechanism is combined with a variety of sensors to collect movement process data, accurately deliver the liquid, and automatically process the movement in the liquid phase preparation process.

(4) The microfluidic liquid phase mixing mechanism is compatible with various types of syringes, and is equipped with a redeveloped software debugging system. The design can be tested for various parameters of the newly added syringe, and after the test is completed, the syringe can be added to be used as the utility model.

(5) The utility model can be used for the preparation of liquid-phase mixed samples with a higher flow rate, for example, the preparation of LNP with a flow rate higher than 16ml/min. The utility model uses a driving motor with a larger torque, and adopts a redesigned software system and power supply system. The driving motor adopted in the utility model can realize high-precision and large-load driving injection and pushing mechanism. The software system used in the utility model can further subdivide the pulse transmission of the driving unit, so as to realize the mixing of the liquid phase at a high flow rate.

(6) The liquid contact mechanism adopts a semi-circular circular motion mechanism, and two sets of clamp structures for collecting test tubes are set. The collection test tube fixture structure adopts a 200-degree arc structure design, which is convenient for fixing and picking and placing the test tube or centrifuge tube for collecting liquid. The semicircular mechanism is arranged around the microfluidic liquid phase mixing mechanism, which not only saves the structure space, but also perfectly realizes the receiving and replacement movement of waste liquid and target liquid. The design has a symmetrical structure style, the equipment has a balanced counterweight, the equipment operates stably, and the appearance design is beautiful.

(7) The housing of the utility model adopts the design of opening the door on the left side, which is more ergonomic and more convenient to use and operate.

(8) The flow rate of the mixed liquid phase of the present invention ranges from 0 to 200ml/min, and has a wider application range. It can be used in various business scenarios such as pre-process exploration and post-process amplification.

Description of drawings

The following drawings are only intended to illustrate and explain the utility model schematically, and do not limit the scope of the utility model. in:

Fig. 1 is a schematic diagram of the structure of a microfluidic device of the present invention in the open state of the door;

Fig. 2 is a schematic structural diagram of a microfluidic device of the present invention in the state of opening and closing the door;

3 is a schematic diagram of the internal structure of a microfluidic device of the present invention;

Fig. 4 is a partially enlarged view of a liquid pushing mechanism of a microfluidic device of the present invention;

Fig. 5 is one of the partially enlarged views of the liquid-contacting mechanism of a microfluidic device of the present invention;

Fig. 6 is the second partial enlarged view of the liquid-contacting mechanism of a microfluidic device of the present invention;

Fig. 7 is a structural schematic diagram of a microfluidic liquid phase mixing mechanism of a microfluidic device of the present invention;

Fig. 8 is a schematic structural view of a pushing fluid pusher of a microfluidic device of the present invention;

9 is a schematic diagram of the internal structure of a chip of a microfluidic device of the present invention;

Fig. 10 is a schematic diagram of the external structure of a chip of a microfluidic device of the present invention;

In the figure: 1-housing; 2-touch screen; 3-push liquid mechanism; 31-push liquid mount; 32-slide screw; 33-fader body; 34-initial position detection sensor; 35-fader contact plate Bearing; 36-fader contact plate; 37-fader support structure; 38-trigger sensor; 4-microfluidic liquid phase mixing mechanism; 41-chip; 42-microfluidic mixing channel; 43-liquid inlet; 44-outlet Liquid port; 45-syringe placement connection hole; 46-injection syringe; 5-liquid contact mechanism; 51-support body of mixed liquid receiving device; 52-rotary liquid receiving structure; The driving gear of the liquid receiving mechanism; 55-the fixture structure of the collection test tube; 56-the liquid-contacting centrifuge tube; 57-the sample collection centrifuge tube; 6-opening the door.

Detailed ways

Below in conjunction with accompanying drawing and embodiment, further set forth the utility model. In the following detailed description, certain exemplary embodiments of the present invention are described by way of illustration only. Needless to say, those skilled in the art would realize that the described embodiments may be modified in various different ways, all without departing from the spirit and scope of the present invention. Accordingly, the drawings and description are illustrative in nature and not intended to limit the scope of the claims.

As shown in Figures 1 to 10, a microfluidic device includes a housing 1, a liquid pushing mechanism 3 is installed inside the housing 1, and a microfluidic liquid phase mixing mechanism 4 is communicated above the liquid pushing mechanism 3, The bottom of the microfluidic liquid phase mixing mechanism 4 is connected with a liquid receiving mechanism 5, and the upper part of the housing 1 is equipped with a touch screen interactive control mechanism, which controls and connects the pushing liquid mechanism 3, the microfluidic liquid phase mixing mechanism 4 and the contactor. The liquid mechanism 5 ; the liquid pushing mechanism 3 and the liquid receiving mechanism 5 are all accommodated in the casing 1 ; the microfluidic liquid phase mixing mechanism 4 includes two liquid inlets 43 , a microfluidic mixing channel 42 and a liquid outlet 44 . The liquid inlet 43 and the liquid outlet 44 are connected through the microfluidic mixing channel 42 , the liquid pushing mechanism 3 can push the raw material liquid into the liquid inlet 43 , and the liquid receiving mechanism 5 can receive the microfluidic mixed liquid from the liquid outlet 44 .

The utility model has the following advantages:

(1) The equipment has a simple structure, a high degree of automation, a friendly interactive solution, and high preparation accuracy. The utility model achieves precise control of the mixing ratio of the two groups of liquid phases through automatic and precise control of the feed volume and speed of the raw liquids of the two channels into the micro-flow liquid phase mixing mechanism 4 .

(2) The touch screen interactive control mechanism, including the touch screen 2, the micro-control chip 41, various sensor components, etc., constitutes a complete system. The UI interface is beautiful, intuitive, and easy to operate, and the feedback of the working status and process is intuitive and clear.

(3) The liquid pushing mechanism 3 is combined with a variety of sensors to collect movement process data, accurately deliver the liquid, and automatically handle the movement in the liquid phase preparation process.

(4) The microfluidic liquid-phase mixing mechanism 4 is compatible with various types of liquid-feeding syringes 46, and is equipped with a newly developed software debugging system. This design can be tested for various parameters of the newly added fluid injector 46, and can be used as the utility model after the test is completed.

(5) The utility model can be used for the preparation of liquid-phase mixed samples with a higher flow rate, for example, the preparation of LNP with a flow rate higher than 16ml/min. The utility model uses a driving motor with a larger torque, and adopts a redesigned software system and power supply system. The driving motor adopted in the utility model can realize high-precision and large-load driving injection and pushing mechanism. The software system used in the utility model can further subdivide the pulse transmission of the driving unit, so as to realize the mixing of the liquid phase at a high flow rate.

(6) The liquid-contacting mechanism 5 adopts a semi-circular circular motion mechanism, and is provided with two sets of clamp structures 55 for collecting test tubes. The collection test tube fixture structure 55 adopts a 200-degree arc structure design, which is convenient for fixing and taking and placing the test tube or centrifuge tube for collecting liquid. The semicircular mechanism is arranged around the microfluidic liquid-phase mixing mechanism 4, which not only saves structural space, but also perfectly realizes the receiving and replacement movement of waste liquid and target liquid. The design has a symmetrical structure style, the equipment has a balanced counterweight, the equipment operates stably, and the appearance design is beautiful.

(7) The housing 1 of the utility model adopts the design of opening the door on the left side, which is more ergonomic and more convenient to use and operate.

(8) The flow rate of the mixed liquid phase of the present invention ranges from 0 to 200ml/min, and has a wider application range. It can be used in various business scenarios such as pre-process exploration and post-process amplification.

As shown in Figure 1, Figure 3, Figure 4 and Figure 8, the pushing liquid mechanism 3 includes two pushing liquid mounting seats 31 installed on the inner bottom of the housing 1, and each pushing liquid mounting seat 31 is vertically installed with a Slide table screw rods 32, each slide table screw rod 32 is respectively equipped with a pushing liquid fader, and an initial position detection sensor 34 is installed on the pushing liquid mounting seat 31. The liquid pushing mechanism 3 is used to push the raw material liquid into the microfluidic mixing channel 42 of the chip 41 at a set ratio and speed. Two sets of sliding table screws 32 are longitudinally arranged inside the housing 1, two pushing liquid faders are installed on the two sets of sliding table screws 32 to move with the movement of the screws, and two sets of initial position detection sensors 34 are installed at the ends of the two sets of sliding table screws 32 Fixed position for precise positioning of the initial position of the push fluid fader. When the pushing liquid pusher moves upwards following the sliding table screw 32, it pushes the liquid-feeding syringe 46, pushes the medicine in the two liquid-feeding syringes 46 into the microfluidic liquid phase mixing mechanism 4 for mixing, and the liquid pushing mechanism 3 also includes power Driving device, power driving device mainly adopts the driving technology that slide table screw rod 32 and stepper motor combine. The utility model uses the ball screw slide table with the highest precision to push the two-phase liquid, and then uses the stepping motor to drive the advanced timer of the MCU under program control to accurately output the specified high-frequency PWM waveform, thereby realizing precise control of the pushing speed and distance. The utility model can realize the maximum movement accuracy of 0.0006mm by further subdividing and outputting PWM in the main control software. The power drive mechanism combines the advantages of the sliding table screw 32 and the stepping motor drive technology, thereby realizing automatic precise positioning and pushing of liquid mixing with high precision and high test repeatability.

As shown in Fig. 1, Fig. 3, Fig. 4 and Fig. 8 together, the pushing fluid fader includes a fader body 33 installed on the slide screw 32, and the upper part of the pusher body 33 is hinged with a pusher through a pusher contact plate bearing 35. The sub-contact plate 36 and the lower part of the fader body 33 are fixedly equipped with a fader support structure 37 on which a trigger sensor 38 is installed, and the lower surface of the fader contact plate 36 is in elastic contact with the trigger sensor 38. The fader contact plate 36 is connected to the fader body 33 through the fader contact plate bearing 35, and can move up and down in a small range. The trigger sensor 38 is installed under the fader contact plate 36 and is located inside the fader support structure 37. The trigger sensor 38 elastically supports the fader contact plate 36 .

As shown in Fig. 1, Fig. 3, Fig. 5, Fig. 6 and Fig. 7, the liquid contact mechanism 5 includes a mixed liquid receiving device support body 51 fixedly installed in the upper part of the housing 1, and the bottom surface of the mixed liquid receiving device support body 51 There is a semicircular slot on the top, and a semicircular rotating liquid receiving structure 52 is installed in the semicircular slot. One side of the rotating liquid collecting structure 52 is provided with a rotating liquid collecting mechanism gear 53, and the mixed liquid receiving device supports The bottom surface of the body 51 is also equipped with a rotating liquid collecting mechanism driving gear 54 meshing with the rotating liquid collecting mechanism gear 53 . The liquid-receiving mechanism 5 has a unique circular motion structure. The rotating liquid-receiving mechanism driving gear 54 meshes with the rotating liquid-receiving mechanism gear 53 provided on the rotating liquid-receiving structure 52 to drive the rotating liquid-receiving structure 52 to achieve left-right rotation. The collection test tube fixture structure 55 is arranged in the middle of the semicircle of the driven rotating liquid collection structure 52 , on which a liquid-receiving centrifuge tube 56 and a sample collection centrifuge tube 57 can be installed for receiving the mixed liquid output from the chip 41 .

As shown in Fig. 1, Fig. 3, Fig. 5 and Fig. 6, the middle part of the support body 51 of the mixed liquid receiving device is provided with two collection test tube fixture structures 55, and the liquid-contacting centrifuge tubes 56 are respectively installed on the collection test tube fixture structures 55. And sample collection centrifuge tube 57. At the initial stage of the two-liquid-phase bolus injection, the mixed liquid before the mixing ratio reaches the set parameter is collected as waste liquid by the liquid-contacting centrifuge tube 56 . The samples when the set mixing parameters are reached are collected by the sample collection centrifuge tube 57 . The liquid contact centrifuge tube 56 and the sample collection centrifuge tube 57 can be automatically switched by the system according to parameter settings.

As shown in Fig. 1, Fig. 3, Fig. 5, Fig. 7, Fig. 9 and Fig. 10, the microfluidic liquid phase mixing mechanism 4 includes a chip 41 installed on the top of the support body 51 of the mixed liquid receiving device, and the chip 41 is provided with micro One end of the microfluidic mixing channel 42 is provided with two liquid inlets 43, the other end of the microfluidic mixing channel 42 is provided with a liquid outlet 44, and the middle position of the rotating liquid receiving structure 52 is provided with two The syringe placement connection hole 45 corresponding to the liquid inlet 43 is respectively equipped with a liquid inlet syringe 46 in the syringe placement connection hole 45, and the liquid outlet 44 is used in conjunction with the liquid contact centrifuge tube 56 and the sample collection centrifuge tube 57. The microfluidic liquid phase mixing mechanism 4 can be used in conjunction with the internal Y-shaped structure chip 41 and the T-shaped structure chip 41 . As shown in Figure 9, taking the Y-shaped structure chip 41 as an example for illustration, the microfluidic liquid phase mixing mechanism 4 has two threaded interfaces, that is, two liquid inlets 43, which can realize tight connection with two liquid inlet syringes 46. connection to ensure no leakage. Under the precise driving of the liquid pushing mechanism 3 , the two-phase liquid can be rapidly mixed in the microfluidic liquid phase mixing mechanism 4 .

As shown in Fig. 1 and Fig. 2 together, an opening is opened on the front of the casing 1, and a door 6 is installed on the opening. The housing 1 adopts the design of opening the door on the left side, which is more ergonomic and more convenient to use and operate.

As shown in Figure 1 and Figure 2 together, the touch screen 2 of the touch screen interactive control mechanism forms an angle of 75° with the horizontal plane. The touch screen interactive control mechanism is used to set the liquid propulsion volume, propulsion speed ratio, total sample flow rate, waste liquid volume, and collected sample volume of the utility model. The touch screen interactive control mechanism adopts The core STM32F7 series ultra-high performance MCU platform. Through the touch screen interactive control UI to set the sample preparation parameters, realize the control of the drive and the control of various sensors, thereby realizing the automatic operation of the equipment.

Work process of the present utility model:

Connect the two liquid inlet syringes 46 to the two liquid inlets 43 of the chip 41 through the syringe placement connection holes 45; place the liquid contact centrifuge tube 56 and the sample collection centrifuge tube 57 in the collection test tube fixture structure 55, and follow the rotation The liquid receiving structure 52 rotates; then, the two-phase liquid volumes are set to 3ml and 12ml respectively on the touch screen interactive control mechanism, the total flow rate is 16ml/min, and the waste liquid collection is 0.5ml. Then, through the initialization setting, the two pushing liquid faders move with the screw rods through two sets of slide table screws 32, and are precisely positioned at the initial positions of the pushing liquid faders under the control of two sets of initial position detection sensors 34. Finally, through the start-up procedure of the touch-screen interactive control mechanism, the pushing liquid pusher pushes the two-phase liquid according to the set ratio and flow rate through two sets of sliding table screws 32, and at the same time, the liquid-contacting centrifuge tube 56 collects the set volume of waste liquid Finally, by rotating the liquid collection mechanism, the drive gear 54 moves out of the liquid contact position, and the sample collection centrifuge tube 57 moves to the liquid contact position for sample collection. After completing the setting program and sample preparation, the liquid pushing movement will stop, and the sample collection centrifuge tube 57 can be removed. During the two-phase liquid pushing process, if one of the liquid phases is lower than the set parameter, the liquid pushing mechanism 3 will stop moving in advance.

The utility model uses the sliding table screw 32 in combination with a stepping motor, and uses The core STM32F7 series ultra-high-performance MCU platform develops a set of touch screen interactive control UI, precise real-time stepping motor drive, and a variety of sensor drivers work closely together to realize automatic and precise positioning and pushing of liquid mixing devices. Stepper motor drive: Write a program to drive the advanced timer of the MCU to accurately output the specified high-frequency PWM waveform to achieve precise control of the pushing speed and distance. MCU can output PWM frequency range: 20HZ～100KHZ. In the main control software, by further subdividing and outputting PWM, the maximum movement accuracy can be realized to be 0.0006mm. Significant for precise mixing of two-phase liquids.

The working process of the core components of the utility model: the amount and speed of the pushing liquid of the two channels are respectively set in the touch screen interactive control mechanism, and the system obtains the set value, which is converted into the corresponding PWM quantity and PWM frequency through calculation. Then the data is sent to the driver, and the specific timer-related register data is set in the MCU, and then the timer is started to work. The timer work does not occupy the main process of the MCU, which avoids the timing impact caused by the main thread running on the accuracy of driving the stepper motor. Therefore, the stepper motors of the two channels start to move according to the setting, and push the syringes of the respective channels to mix through the chip 41 .

The unique circular motion structure of the utility model is used for collecting waste liquid and target samples. Its structure is simple and ingenious, which can effectively reduce the volume of the equipment, and the symmetrical structure style is conducive to the balance of the counterweight of the equipment, improves the stability of the equipment operation, and improves the design aesthetics.

At the beginning of the design of the utility model, the single mixing capacity, speed, and use scenarios compatible with various types of syringes have been taken into consideration. In order to meet the situation of large-capacity mixing, the design uses a screw slide mechanism with a larger effective stroke, and the effective load is also increased accordingly. The equipment software system is also optimized and adapted for large-capacity mixing. A series of improvement measures ensure that the equipment can effectively complete the large-capacity liquid-phase mixing requirements.

The utility model is compatible with various types of syringes, and the software system has developed a set of debugging system for testing various parameters of the newly added syringes. After the test is completed, the syringe can be added and used as the device.

The utility model can not only be used for the screening of low-flow-velocity nano-medicine lipids in the range of 1-20ml/min in the early stage, but also solves the problem that the traditional microfluidic control device cannot be scaled up, and the maximum flow rate can support up to 200ml/min. min.

The utility model has the advantages of simple equipment structure, high degree of automation, convenient operation, and high preparation precision. The microfluid mixed liquid is prepared by a micro-mixing device, and the amount and speed of the raw material liquid entering the micro-mixing device are precisely controlled through automation. , to accurately control the ratio of the various raw material liquids, in addition, the different liquid receiving tubes of the liquid receiving mechanism 5 can be automatically switched, and the multi-dose continuous preparation of the prepared microfluidic mixture can be realized.

The utility model prepares the classic LNP prescription situation table:

Through this utility model, the cationic Dlin-MC3-DMA classic LNP prescription is adopted, and four components including cationic, cholesterol, auxiliary phospholipid DOPC, and PEG2000-DSPE are dissolved in ethanol according to the molar ratio of 50:38:10:2 to obtain 10ml lipid ethanol solution; Dilute the pDNA plasmid expressing firefly luciferase in the sodium acetate solution of pH 4, set the parameters according to the following table 1 to obtain pDNA/MC3LNP, the particle size and distribution of the obtained LNP And the encapsulation efficiency is shown in Table 1 below.

Table 1 Preparation of classic LNP prescription situation table

The above-mentioned PDI represents the dispersibility index of the particles, and the results of the data confirm that the DNA-encapsulating gene drug obtained by the utility model has a small particle size, good dispersibility, and high encapsulation efficiency. The pDNA/MC3 LNP prepared by the utility model shows a good pDNA delivery effect in the in vivo experiment of mice, and the expression level of firefly luciferase in the mice is correlated with the injection dose, and has a stable expression within 14 days.

This application involves the technical features of software and circuit programs, and the realization of its functions belongs to the prior art. The essence of the technical solution of this application is to improve the composition and connection relationship of hardware parts, and does not involve the software program or circuit structure itself. Improve.

In describing the present utility model, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", The orientation or positional relationship indicated by "outside" is based on the orientation or positional relationship shown in the drawings, which is only for the convenience of describing the utility model and simplifying the description, rather than indicating or implying that the referred device or element must have a specific orientation , are constructed and operated in a specific orientation and therefore cannot be construed as limiting the invention.

The basic principles, main features and advantages of the present utility model have been shown and described above. Those skilled in the industry should understand that the utility model is not limited by the above-mentioned embodiments. The above-mentioned embodiments and descriptions only illustrate the principles of the utility model. Without departing from the spirit and scope of the utility model, the utility model The new model also has various changes and improvements, and these changes and improvements all fall within the scope of the claimed utility model. The scope of protection required by the utility model is defined by the appended claims and their equivalents.

Claims (9)

1. Microfluidic device comprising a housing (1), characterized in that: internally mounted of casing (1) has propelling movement liquid mechanism (3), the top intercommunication of propelling movement liquid mechanism (3) has little stream liquid phase mixing mechanism (4), the below intercommunication of little stream liquid phase mixing mechanism (4) has liquid receiving mechanism (5), touch screen interaction control mechanism is installed on the upper portion of casing (1), touch screen interaction control mechanism control connects propelling movement liquid mechanism (3 little stream liquid phase mixing mechanism (4) with liquid receiving mechanism (5).

2. A microfluidic device according to claim 1, wherein: the pushing liquid mechanism (3) comprises two pushing liquid installation seats (31) installed at the inner bottom of the shell (1), a sliding table screw rod (32) is vertically installed on each pushing liquid installation seat (31), pushing liquid clippers are installed on each sliding table screw rod (32), and an initial position detection sensor (34) is installed on each pushing liquid installation seat (31).

3. A microfluidic device as claimed in claim 2, wherein: the pushing liquid clipper comprises a clipper body (33) arranged on the sliding table screw rod (32), a clipper contact plate (36) is hinged to the upper portion of the clipper body (33) through a clipper contact plate bearing (35), a clipper support structure (37) is fixedly arranged on the lower portion of the clipper body (33), a trigger sensor (38) is arranged on the clipper support structure (37), and the lower surface of the clipper contact plate (36) is in elastic contact with the trigger sensor (38).

4. A microfluidic device according to claim 1, wherein: the liquid receiving mechanism (5) comprises a mixed liquid receiving device supporting body (51) fixedly installed at the inner upper part of the shell (1), a semicircular groove is formed in the bottom surface of the mixed liquid receiving device supporting body (51), a semicircular annular rotary liquid receiving structure body (52) is installed in the semicircular groove, a rotary liquid receiving mechanism gear (53) is arranged on one side of the rotary liquid receiving structure body (52), and a rotary liquid receiving mechanism driving gear (54) meshed with the rotary liquid receiving mechanism gear (53) is further installed on the bottom surface of the mixed liquid receiving device supporting body (51).

5. A microfluidic device according to claim 4, wherein: the middle part of the mixed liquid receiving device support body (51) is provided with two collection test tube clamp structures (55), and a liquid receiving centrifuge tube (56) and a sample collection centrifuge tube (57) are respectively arranged on the collection test tube clamp structures (55).

6. A microfluidic device according to claim 5, wherein: the micro-fluid liquid phase mixing mechanism (4) comprises a chip (41) arranged at the top of a support body (51) of the mixed liquid receiving device, a micro-fluid mixing channel (42) is arranged inside the chip (41), two liquid inlets (43) are formed in one end of the micro-fluid mixing channel (42), a liquid outlet (44) is formed in the other end of the micro-fluid mixing channel (42), two injector placing connecting holes (45) corresponding to the liquid inlets (43) are formed in the middle of the rotary liquid receiving structure (52), liquid inlet injectors (46) are respectively arranged in the injector placing connecting holes (45), and the liquid outlet (44) is matched with the liquid receiving centrifugal tube (56) and the sample collecting centrifugal tube (57).

7. A microfluidic device according to claim 6, wherein: the chip (41) is a Y-shaped structure chip or a T-shaped structure chip.

8. A microfluidic device according to claim 1, wherein: an opening is formed in the front of the shell (1), and a door opening (6) is arranged on the opening.

9. A microfluidic device according to any one of claims 1 to 6, wherein: the touch screen (2) of the touch screen interaction control mechanism forms an angle of 75 degrees with the horizontal plane.

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