CN217973193U - Cell culture bottle with replaceable filter membrane - Google Patents

Abstract

The utility model proposes a cell culture bottle with a replaceable filter membrane, which includes a bottle body and a bottle cap, the bottle cap includes an upper cover and a lower cover, and a micropore hydrophobic is arranged between the upper cover and the lower cover. The filter membrane, the upper cover and the lower cover are connected by threads, the upper cover is located outside the lower cover, the bottle body includes a bottle body and a bottle mouth, and the lower cover and the bottle mouth are connected by threads. The culture bottle has a simple structure, is easy to use, can realize low-cost repeated use, and is beneficial to reduce the cost of cell culture.

Description

Replaceable filter membrane cell culture flask

technical field

The utility model relates to the technical field of cell culture devices, in particular to a replaceable membrane cell culture bottle.

Background technique

At present, in vitro cell experiments involved in the field of biomedicine often require cell culture, and cell culture containers are generally used as cell culture containers. Currently, the common cell culture bottles on the market are divided into two categories, recyclable glass cell culture bottles and disposable aseptic plastic culture bottles. Among them, the glass cell culture bottle includes a glass bottle body and a matching high-temperature-resistant bottle cap, which can achieve the aseptic effect required for cell experiments after high-pressure sterilization and ultraviolet irradiation. Disposable aseptic plastic culture bottles include aseptic plastic bottles and aseptic plastic bottle caps, wherein aseptic plastic bottle caps are divided into bottle caps with filter membranes and bottle caps without filter membranes.

As far as the effect of cell culture is concerned, the environment inside the bottle cap with a filter membrane can communicate with the external environment, especially in the cell incubator, which can stably control the concentration of carbon dioxide, which is conducive to promoting cell growth. But it can only be used once, and the cost of cell culture is relatively high. There is no product on the market that can increase the communication between the inside and outside of the bottle without a filter membrane and can be used repeatedly. At most, the bottle cap can only be loosened when culturing cells, but this greatly increases the chance of cell culture contamination. risk.

Contents of the invention

In order to overcome the deficiencies of the prior art, the utility model proposes a replaceable filter membrane cell culture bottle, which has a simple structure, is easy to use, can realize low-cost repeated use, and is beneficial to reduce the cost of cell culture.

In order to achieve the above object, the replaceable filter membrane cell culture bottle of the present invention includes a bottle body and a bottle cap, the bottle cap includes an upper cover and a lower cover, a microporous hydrophobic filter is arranged between the upper cover and the lower cover, and the upper cover It is threadedly connected with the lower cap, the upper cap is located outside the lower cap, the bottle body includes a bottle body and a bottle mouth, and the lower cap and the bottle mouth are connected by threads.

Further, the bottom of the lower cover is provided with a protruding part, and the outer diameter of the protruding part is larger than that of the upper cover.

Further, the top opening of the lower cover, the top opening of the upper cover, the inner diameter of the lower cover opening and the inner diameter of the upper cover opening are all less than the diameter of the microporous hydrophobic filter membrane, and the microporous hydrophobic filter membrane is arranged on the opening of the upper cover and the opening of the lower cover. In between, the opening of the upper cover is also provided with limiting ribs for limiting the microporous hydrophobic filter membrane.

Further, the top of the upper cover is also provided with a sealing groove, the sealing groove is located below the opening of the upper cover, the inner diameter of the sealing groove is larger than the outer diameter of the microporous hydrophobic filter membrane, and an O-ring is arranged in the sealing groove, and the O-ring fits On the periphery of the microporous hydrophobic filter membrane.

Further, the bottle mouth is arranged obliquely on the bottle body, and the top of the bottle mouth is outside the edge of the bottle body.

Further, the microporous hydrophobic filter membrane is disc-shaped, and the pore diameter of the microporous hydrophobic filter membrane is 0.2 μm.

The replaceable filter membrane cell culture bottle of the utility model has a simple structure, is easy to use, can realize low-cost repeated use, and is beneficial to reduce the cost of cell culture.

Description of drawings

Below in conjunction with accompanying drawing, the utility model is further described and set forth.

Fig. 1 is a schematic diagram of the overall structure of a replaceable filter membrane cell culture bottle in the preferred embodiment of the present invention.

Fig. 2 is a structural schematic diagram for embodying the bottle cap.

Fig. 3 is a sectional view showing the structure of the bottle cap.

Fig. 4 is an enlarged view at point A in Fig. 3 .

Reference signs: 1, bottle body; 11, bottle body; 12, bottle mouth; 2, bottle cap; 21, upper cover; 211, sealing groove; 212, limit rib; 22, lower cover; 221, protruding 3. Microporous hydrophobic membrane; 4. O-ring.

detailed description

The technical solution of the utility model will be explained more clearly and completely through the description of preferred embodiments of the utility model in conjunction with the accompanying drawings.

As shown in Figure 1, the replaceable filter membrane cell culture bottle of the preferred embodiment of the present utility model includes a bottle body 1 and a bottle cap 2, the bottle body 1 includes a bottle body 11 and a bottle mouth 12, and the bottle cap 2 is arranged on the bottle mouth 12 superior. Both the bottle body 1 and the bottle cap 2 can be sterilized by high-pressure moist heat, so as to achieve the effect of repeated use.

The bottle mouth 12 is obliquely arranged on the bottle body 11, and the top of the bottle mouth 12 is outside the edge of the bottle body 11. When the bottle body 1 is placed horizontally like this, keep the bottle mouth 12 above the liquid level of the culture liquid, avoid the culture liquid from contacting the bottle cap 2, thereby reducing the occurrence of culture liquid pollution.

As shown in Fig. 3 and Fig. 4, the bottle cap 2 includes an upper cap 21 and a lower cap 22, the upper cap 21 is sleeved on the outside of the lower cap 22, and the two are connected by threads. The lower cover 22 is threadedly connected to the bottle mouth 12 . The bottom of lower cover 22 is provided with projection 221, and the outer diameter of projection 221 is greater than the external diameter of upper cover 21, and promptly projection 221 can protrude beyond upper cover 21, and operator can select like this to be bottle cap 2 The whole is taken off from the bottle body 1, or the upper cover 21 is separated from the lower cover 22 to avoid misuse.

As shown in Figures 3 and 4, the top opening of the lower cover 22, the top opening of the upper cover 21, and the opening of the upper cover 21 is provided with a disc-shaped microporous hydrophobic filter membrane 3 with an aperture of 0.2 μm between the openings of the lower cover 22. . The inner diameter of the lower cover 22 opening and the inner diameter of the upper cover 21 opening are all less than the diameter of the microporous hydrophobic filter membrane 3, and the upper cover 21 opening is also provided with a limiting rib 212 for limiting the microporous hydrophobic filter membrane 3, so that The microporous hydrophobic filter membrane 3 is well limited between the upper cover 21 and the lower cover 22 .

As shown in Figures 2 and 4, in addition, the top of the upper cover 21 is also provided with a sealing groove 211, the sealing groove 211 is located below the opening of the upper cover 21, and the inner diameter of the sealing groove 211 is greater than the outer diameter of the microporous hydrophobic filter membrane 3, An O-ring 4 is arranged in the sealing groove 211, and the O-ring 4 is attached to the outer periphery of the microporous hydrophobic filter membrane 3, and the sealing degree is improved through the O-ring 4, so that the air can only circulate through the microporous hydrophobic filter membrane 3, Improve filtration efficiency and avoid culture contamination.

When the replaceable filter membrane cell culture bottle of the present utility model is reused, it is only necessary to separate the upper cover 21 from the lower cover 22 and then discard the used microporous hydrophobic filter membrane 3, which is sterilized simultaneously with the bottle body 1, and after disinfection, the upper layer Just can use by packing into new sterilization microporous hydrophobic filter membrane 3 between cover 21 and lower floor cover 22. Only the microporous hydrophobic filter membrane 3 needs to be replaced, and the bottle body 1 and the bottle cap 2 can be reused many times, which greatly reduces the cost of cell culture.

The above-mentioned specific embodiments only describe the preferred embodiments of the present utility model, but do not limit the protection scope of the present utility model. Under the premise of not departing from the design concept and spirit category of the present utility model, those skilled in the art may make various deformations, substitutions and improvements to the technical solution of the present utility model according to the written description and accompanying drawings provided by the utility model, All should belong to the protection category of the present utility model. The protection scope of the utility model is determined by the claims.

Claims (6)

1. a replaceable membrane cell culture bottle, is characterized in that, comprises bottle body (1) and bottle cap (2), and described bottle cap (2) comprises upper cover (21) and lower floor cover (22), so A microporous hydrophobic filter membrane (3) is provided between the upper cover (21) and the lower cover (22), and the upper cover (21) and the lower cover (22) are connected by threads, and the upper cover (21) is located at The outside of the lower cover (22), the bottle body (1) includes a bottle body (11) and a bottle mouth (12), and the lower cover (22) and the bottle mouth (12) are connected by threads. 2. replaceable filter membrane cell culture bottle according to claim 1, is characterized in that, the bottom of described lower cover (22) is provided with protruding part (221), and the outer diameter of described protruding part (221) Greater than the outer diameter of the upper cover (21). 3. replaceable membrane cell culture bottle according to claim 1, is characterized in that, the top opening of described lower cover (22), the top opening of described upper cover (21), the described lower cover (22) The inner diameter of the opening and the inner diameter of the upper cover (21) opening are all less than the diameter of the microporous hydrophobic filter membrane (3), and the microporous hydrophobic filter membrane (3) is arranged on the opening of the upper cover (21) and the opening of the lower cover (22). Between, the opening of the upper cover (21) is also provided with limiting ribs (212) for limiting the microporous hydrophobic filter membrane (3). 4. replaceable filter membrane cell culture flask according to claim 2, is characterized in that, the top of described upper layer cover (21) is also provided with sealing groove (211), and described sealing groove (211) is positioned at upper layer cover ( 21) Below the opening, the inner diameter of the sealing groove (211) is greater than the outer diameter of the microporous hydrophobic filter membrane (3), the sealing groove (211) is provided with an O-ring (4), and the O-ring (4) Attached to the periphery of the microporous hydrophobic filter membrane (3). 5. The replaceable filter membrane cell culture bottle according to claim 1, characterized in that, the bottle mouth (12) is obliquely arranged on the bottle body (11), and the top of the bottle mouth (12) Outside the bottle body (11) edge. 6. replaceable filter membrane cell culture flask according to claim 1, is characterized in that, described microporous hydrophobic filter membrane (3) is disk shape, and the aperture of described microporous hydrophobic filter membrane (3) 0.2 μm.

Images