CN217836833U

CN217836833U - High-efficient reverse transcription kit

Info

Publication number: CN217836833U
Application number: CN202222132279.1U
Authority: CN
Inventors: 王海冰
Original assignee: Zhejiang Yiside Biotechnology Co ltd
Current assignee: Zhejiang Yiside Biotechnology Co ltd
Priority date: 2022-08-13
Filing date: 2022-08-13
Publication date: 2022-11-18
Anticipated expiration: 2032-08-13

Abstract

The utility model discloses a high-efficient reverse transcription kit, including the kit body, the inside mounting bracket that is equipped with of kit body, mounting bracket and the cooperation of pegging graft of kit body, the mounting bracket top is equipped with the glass board, glass board and kit body normal running fit, a plurality of holding tanks have been seted up at the mounting bracket top, a plurality of ventilation holes have been seted up to the holding tank bottom, all be equipped with clamping device in a plurality of holding tanks, clamping device and mounting bracket fixed connection, the mounting bracket bottom is equipped with cooling device, cooling device and kit body cooperation of sliding, the standing groove has been seted up to the mounting bracket both sides, kit one side is equipped with the kit lid, kit lid and kit body lock joint cooperation, the utility model discloses rational in infrastructure, the activity of reverse transcriptase is reduced in cooling through cooling device, effectively prevents the unable experiment of deactivation, the cooling device's of being convenient for more change, clamping device can make the test tube store more firm in the kit.

Description

High-efficient reverse transcription kit

Technical Field

The utility model relates to a biological kit technical field, in particular to high-efficient reverse transcription kit.

Background

Reverse transcription is a process of synthesizing DNA using RNA as a template, i.e., DNA synthesis under RNA guidance. In this process, the process of nucleic acid synthesis and transcription (DNA to RNA) is opposite to the flow direction of genetic information (RNA to DNA), and is called reverse transcription. The reverse transcription process is one of the replicative forms of RNA viruses and is catalyzed by reverse transcriptase.

The reverse transcription kit is needed to be used when RNA template reverse transcription is carried out, the conventional reverse transcription kit generally simply places a test tube, reverse transcriptase gradually loses activity in a high-temperature environment, so that the reverse transcription effect cannot be finished, the test tube does not comprise a fastening device in the kit, and a solution is provided aiming at the problems.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a high-efficient reverse transcription kit solves the problem mentioned in the background art, and cooling device reduces reverse transcriptase's activity, prevents the inactivation of reverse transcriptase, can make the test tube in the reagent box keep firm when removing through clamping device.

The above technical purpose of the present invention can be achieved by the following technical solutions:

the utility model provides a high-efficient reverse transcription kit, includes the kit body, the inside mounting bracket that is equipped with of kit body, mounting bracket and the cooperation of pegging graft of kit body, the mounting bracket top is equipped with the glass board, glass board and kit body normal running fit, a plurality of holding tank have been seted up at the mounting bracket top, a plurality of ventilation holes have been seted up to the holding tank bottom, and are a plurality of all be equipped with clamping device in the holding tank, clamping device and mounting bracket fixed connection, the mounting bracket bottom is equipped with cooling device, cooling device and kit body cooperation of sliding, the standing groove has been seted up to the mounting bracket both sides, kit one side is equipped with the kit lid, kit lid and kit body lock joint cooperation.

Adopt above-mentioned technical scheme, the test tube is placed in the holding tank through the mounting bracket, the mounting bracket can effectively avoid test tube collision each other to cause the damage, stabilize test tube bottom through clamping device, cooling device brings cold wind into to the holding tank in through the ventilation hole, guarantee that the reverse transcriptase in the test tube is in low temperature environment all the time, keep its activity, cooling device can change the cooling material at any time through sliding, the kit lid can guarantee that the kit keeps encapsulated situation, can place the required instrument of experiment in the standing groove.

As preferred, clamping device includes spring and swash plate, seted up a plurality of resettlement grooves in the holding tank, the spring is arranged in the resettlement groove, the swash plate is arranged in resettlement groove one side, swash plate and holding tank normal running fit, spring one end and resettlement groove fixed connection, the spring other end and swash plate fixed connection.

Adopt above-mentioned technical scheme, put into the holding tank with the test tube in, the spring receives the extrusion, can contract to the mounting groove in, and the swash plate tends to the laminating of holding tank inner wall, and when the test tube was taken out, the spring was through restoring to the throne with the swash plate with hold the groove top to being certain angle.

Preferably, the mounting frame is provided with a plurality of through holes, the inner walls of the through holes are provided with rubber pads, and the rubber pads are fixedly connected with the through holes.

Adopt above-mentioned technical scheme, the rubber pad in the through-hole can effectively reduce the test tube and place the collision wearing and tearing on the mounting bracket.

As preferred, cooling device includes baffle, box body and handle, the baffle is arranged in the mounting bracket bottom, baffle and the cooperation of kit body joint, the box body is arranged in the baffle top, the cooperation of sliding of box body and kit body, the handle is arranged in box body one side, handle and box body fixed connection.

Adopt above-mentioned technical scheme, the baffle can avoid with cooling water direct contact to can bring air conditioning into the mounting bracket through the baffle, can change the processing with the cooling water in the box body through the handle.

Preferably, a protection pad is arranged on one side of the inclined plate and fixedly connected with the inclined plate.

Adopt above-mentioned technical scheme, reduce the wearing and tearing of test tube when placing the holding tank.

Preferably, a snap ring is arranged on one side of the kit cover and fixedly connected with the kit cover, a convex block is arranged on one side of the kit body and fixedly connected with the kit body, and the snap ring is matched with the convex block in a buckling manner.

By adopting the technical scheme, the retaining ring is matched with the lug. The opening and closing of the kit cover are convenient, and the operation of experimenters is convenient.

Preferably, a sealing ring is arranged on one side, close to the glass plate, of the kit cover, and the sealing ring is detachably connected with the kit cover.

Adopt above-mentioned technical scheme, help the inside sealed environment that keeps of kit body.

Has the beneficial effects that: the cooling water in the cooling device can effectively keep low temperature to this internal test tube of kit for reverse transcriptase in the test tube has activity all the time, the required research of experiment of being convenient for, cooling device accessible box body is changed it, the laboratory technician's of being convenient for save time, the change process need not to take the test tube, will place in the test tube of holding tank and fasten through the effect of swash plate and spring, test tube collision each other or rock and overturn in that the kit is originally internal when avoiding removing.

Drawings

FIG. 1 is a schematic structural diagram of an embodiment;

FIG. 2 is a schematic diagram showing an internal top view position of the embodiment;

FIG. 3 is a front sectional view of the embodiment;

FIG. 4 is a sectional view of the embodiment;

FIG. 5 is an enlarged view of the embodiment showing the direction C;

FIG. 6 is an enlarged view of the embodiment shown in the direction A;

fig. 7 is an enlarged view of the embodiment showing the direction B.

Reference numerals are as follows: 1. a kit body; 2. a mounting frame; 3. a glass plate; 4. accommodating grooves; 5. a vent hole; 6. a clamping device; 7. a cooling device; 8. a placement groove; 9. a kit cover; 10. a spring; 11. a sloping plate; 12. a placing groove; 13. a through hole; 14. a rubber pad; 15. a partition plate; 16. a case body; 17. a handle; 18. a protective pad; 19. a retaining ring; 20. a bump; 21. and (5) sealing rings.

Detailed Description

The following is only the preferred embodiment of the present invention, the protection scope is not limited to this embodiment, and all technical solutions belonging to the idea of the present invention should belong to the protection scope of the present invention. It should also be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and such modifications and decorations should also be regarded as the protection scope of the present invention.

See fig. 1 to 7, a high-efficient reverse transcription kit, including kit body 1, kit body 1 is inside to be equipped with mounting bracket 2, mounting bracket 2 and kit body 1 grafting cooperation, mounting bracket 2 top is equipped with glass board 3, glass board 3 and kit body 1 normal running fit, a plurality of through-holes 13 have been seted up on mounting bracket 2, a plurality of through-hole 13 inner walls are equipped with rubber pad 14, rubber pad 14 and through-hole 13 fixed connection, a plurality of holding tank 4 have been seted up at mounting bracket 2 top, a plurality of ventilation holes 5 have been seted up to holding tank 4 bottom, ventilation holes 5 can be convenient for the circulation of air, conveniently drive cold and hot air and flow, all be equipped with clamping device 6 in a plurality of holding tank 4, clamping device 6 and mounting bracket 2 fixed connection, clamping device 6 includes spring 10 and swash plate 11, a plurality of arrangement groove 12 have been seted up to holding tank 4, spring 10 arranges in arrangement groove 12, swash plate 11 arranges in arrangement groove 12 one side, swash plate 11 and holding tank 4 normal running fit, swash plate 11 one side is equipped with protection pad 18, protection pad 18 and swash plate 11 fixed connection, the other end of hard material can reduce the friction that the test tube slides, avoid swash plate 11 and test tube wearing and wear.

The bottom of the mounting frame 2 is provided with the cooling device 7, the cooling device 7 is matched with the kit body 1 in a sliding manner, the cooling device 7 comprises a partition plate 15, a box body 16 and a handle 17, the partition plate 15 is arranged at the bottom of the mounting frame 2, the partition plate 15 is matched with the kit body 1 in a clamping manner, the box body 16 is arranged at the top of the partition plate 15, the box body 16 is matched with the kit body 1 in a sliding manner, the handle 17 is arranged on one side of the box body 16, the handle 17 is fixedly connected with the box body 16, the two sides of the mounting frame 2 are provided with the placing grooves 8, one side of the kit is provided with the kit box cover 9, the kit box cover 9 is matched with the kit body 1 in a buckling manner, one side of the kit box cover 9 is provided with a buckle ring 19, the buckle ring 19 is fixedly connected with the kit box cover 9, the kit box body 1 can be conveniently opened and closed through buckling engagement, one side of the kit box body 16 is provided with a bump 20, the bump 20 is fixedly connected with the bump 20, one side of the kit box cover 9 close to the glass plate 3 is provided with a sealing ring 21, the sealing ring 21 and the sealing ring 21 can be detachably connected with the inside of the kit box body 1, and the sealing ring 21 can better enable the environment state of the test tube to be ensured.

Open kit lid 9, the test tube is put into to holding tank 4 from the test tube on the mounting bracket 2 in, swash plate 11 in the holding tank 4 when the test tube is placed, can be compressed back to in the settling tank 12, effort through spring 10 and swash plate 11 is with the test tube centre gripping, fasten the test tube, thereby make the test tube can not tumble in kit body 1 easily when the transport, also can avoid the collision wearing and tearing between the test tube, place the cooling water in cooling device 7, ventilation hole 5 through baffle 15 and holding tank 4 bottom, steam is to the test tube motion in the mounting bracket 2, reduce the ambient temperature around the test tube, guarantee the activity of the reverse transcriptase in the test tube, in the use, can will place the cooling water in box body 16, change through handle 17, effectively keep the temperature in kit body 1 for a long time in the low temperature state, can guarantee that reverse transcriptase is in the low temperature state when storing the test tube, keep its activity.

Claims

1. The utility model provides a high-efficient reverse transcription kit, includes kit body (1), its characterized in that, inside mounting bracket (2) that is equipped with of kit body (1), mounting bracket (2) and kit body (1) cooperation of pegging graft, mounting bracket (2) top is equipped with glass board (3), glass board (3) and kit body (1) normal running fit, a plurality of holding tank (4) have been seted up at mounting bracket (2) top, a plurality of ventilation hole (5) have been seted up to holding tank (4) bottom, and is a plurality of all be equipped with clamping device (6) in holding tank (4), clamping device (6) and mounting bracket (2) fixed connection, mounting bracket (2) bottom is equipped with cooling device (7), cooling device (7) and kit body (1) cooperation of sliding, standing groove (8) have been seted up to mounting bracket (2) both sides, kit one side is equipped with kit lid (9), kit lid (9) and kit body (1) lock joint cooperation.

2. The kit for reverse transcription with high efficiency according to claim 1, wherein the clamping device (6) comprises a spring (10) and an inclined plate (11), a plurality of placing grooves (12) are formed in the accommodating groove (4), the spring (10) is arranged in the placing groove (12), the inclined plate (11) is arranged on one side of the placing groove (12), the inclined plate (11) is in rotating fit with the accommodating groove (4), one end of the spring (10) is fixedly connected with the placing groove (12), and the other end of the spring (10) is fixedly connected with the inclined plate (11).

3. The kit for reverse transcription as claimed in claim 1, wherein the mounting rack (2) is provided with a plurality of through holes (13), rubber pads (14) are provided on inner walls of the plurality of through holes (13), and the rubber pads (14) are fixedly connected with the through holes (13).

4. The high-efficiency reverse transcription kit according to claim 1, wherein the cooling device (7) comprises a partition plate (15), a kit body (16) and a handle (17), the partition plate (15) is arranged at the bottom of the mounting frame (2), the partition plate (15) is in clamping fit with the kit body (1), the kit body (16) is arranged at the top of the partition plate (15), the kit body (16) is in sliding fit with the kit body (1), the handle (17) is arranged on one side of the kit body (16), and the handle (17) is fixedly connected with the kit body (16).

5. The reverse transcription kit according to claim 2, wherein a protective pad (18) is disposed on one side of the tilted plate (11), and the protective pad (18) is fixedly connected with the tilted plate (11).

6. The kit of claim 1, wherein a retaining ring (19) is disposed on one side of the kit cover (9), the retaining ring (19) is fixedly connected with the kit cover (9), a protrusion (20) is disposed on one side of the kit body (16), the protrusion (20) is fixedly connected with the kit body (16), and the retaining ring (19) and the protrusion (20) are in snap fit.

7. The kit for reverse transcription as claimed in claim 1, wherein the side of the kit cover (9) close to the glass plate (3) is provided with a sealing ring (21), and the sealing ring (21) is detachably connected with the kit cover (9).