Detailed Description
In order to facilitate an understanding of the invention, specific embodiments thereof will be described in more detail below with reference to the accompanying drawings.
As used herein, unless otherwise specified or defined, "first, \ 8230," is used merely to distinguish between names, and does not denote a particular quantity or order.
As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items, unless specified or otherwise defined.
It should be noted that "fixed" and "connected" in this document may be directly fixed or connected to one element or may be indirectly fixed or connected to one element.
As shown in fig. 1 to 5, the automatic semen processing station comprises a workstation main body 10, an operating area 101 is arranged on the workstation main body 10, a manipulator 102 is arranged in the operating area 101, and a semen cup placing platform 20, a consumable platform, an incubator, an automatic semen cup sample feeding device 30, a semen operating platform 40, an automatic uncovering device 50 and an automatic semen analyzing device 60 are arranged around the manipulator 102, and a control module electrically connected with the manipulator 102 is arranged in the workstation main body 10. The manipulator 102 is controlled by the control module to enter the semen cup placing table 20, the consumable table, the incubator, the automatic semen cup sample feeding device 30, the semen operation table 40, the automatic cover opening device 50 and the automatic semen analyzing device 60 on the operation area 101, so that the full-automatic semen processing operation is realized.
The control module is a central processing unit. The semen processing method comprises the steps of presetting the semen processing method in a central processing unit through software.
The operation area 101 is a closed operation area or an open operation area; in this embodiment, an open operation area is preferred, and one end of the operation area 101 is arranged in an open manner.
The bottom of the operation area 101 is provided with the manipulator 102, one side of the manipulator 102 is provided with the incubator, and the other side is provided with the automatic semen cup sample feeding device 30, the semen operation table 40 and the automatic semen analysis device 60; the consumable table is disposed at one end of the manipulator 102, and the automatic lid opening device 50 is disposed at the other end.
The incubator includes a first incubator 103 and a second incubator 104, the first incubator 103 is provided at a side portion of the operation area 101 to be embedded in a side portion of the workstation body 10, and the second incubator 104 is provided at a bottom portion of the operation area 101 to be embedded in a bottom portion of the workstation body 10.
The first incubator 103 is provided with an openable door 1031 facing the wall of the operation area 101 for the manipulator 102 to extend into the first incubator 103 for sampling or lofting.
The door body 1031 is a single-side door or a sliding door.
A plurality of layers of tower-type turntables 1032 are arranged in the first incubator 103, and a plurality of clamping grooves 1033 are arranged on the tower-type turntables 1032 and used for fixing the sperm collecting vessel 703.
An openable and closable sampling hole 1041 is formed in a wall of the second incubator 104 facing the operation area 101, so that the manipulator 102 can extend into the second incubator 104 for sampling or lofting.
The sampling hole 1041 adopts an electric control lateral opening and closing structure.
The first incubator 103 and the second incubator 104 are independent single gas incubators or triple gas incubators, and a single gas incubator is preferred in this embodiment. The first incubator 103 is of a single-side door opening structure or a sliding door opening structure, and the temperature, the humidity and the gas concentration inside the first incubator can be adjusted and controlled; the second incubator 104 is of an upper cover-opening structure, and the temperature, the humidity and the gas concentration inside the second incubator can be adjusted and controlled.
The fine cup placing table 20 is fixedly installed on the side of the work station main body 10 opposite to the first incubator 103.
The fine cup placing table 20 is provided with a plurality of fine cup placing layers 201, a plurality of fine cup constant temperature clamping grooves (not shown) are formed in the fine cup placing layers 201 and used for placing fine cups 202, and at least 1/3 or 1/2 of the bottoms of the fine cups 202 are embedded into the fine cup constant temperature clamping grooves.
The fine cup placing table 20 can be used independently.
The side of the workstation main body 10 is provided with a first display screen 105, the top of the workstation main body 10 is provided with a second display screen 106, and the first display screen 105 and the second display screen 106 are used for displaying the operation steps, the sample check pictures, the real-time pictures and the video playback in the working area of the workstation.
A mounting groove 107 is formed in the top of the workstation body 10, the mounting groove 107 is communicated with the operating area 101, and a filtering module 108, an illuminating module (not shown) and a sterilizing module (not shown) are arranged in the mounting groove 107.
The filter module 108 includes a coarse filter (not shown), a high efficiency filter (not shown), and a blower 1081 for filtering and purifying air in the operation area 101.
The lighting module may be an LED lamp, an incandescent lamp, a fluorescent lamp, an energy saving lamp, a halogen tungsten lamp, or the like, as long as the purpose of lighting the operation area 101 can be achieved.
The sterilization module is an ultraviolet sterilization lamp, preferably a low-pressure ultraviolet sterilization lamp, and it should be understood that the sterilization module is not limited to the ultraviolet sterilization lamp of this embodiment as long as the purpose of performing sterilization treatment on the operation area 101 after the operation is completed on a single day can be achieved.
The first display screen 105 and the second display screen 106 of this embodiment are capacitive liquid crystal display screens or touch resistance liquid crystal display screens, and are used for operating and controlling the start and stop of the lighting module and the filtering module, and the parameter display and setting of the first incubator 103 and the second incubator 104.
The top of the operating area 101 is provided with a plurality of monitoring cameras 109 for monitoring and recording semen processing operations in the operating area 101.
The monitoring camera 109 is a CCD (charge coupled device) monitoring camera.
A garbage collection box 110 is arranged in the bottom of the workstation main body 10, a sample dropping hole 111 is formed in the operation area 101, and the sample dropping hole 111 is communicated with the garbage collection box 110.
A first cabinet 112 is arranged at the right side of the bottom of the workstation main body 10, and the garbage collection box 110 is arranged in the first cabinet 112.
An opening/closing mechanism (not shown) capable of electrically opening/closing the sample discarding hole 111 is provided in the first cabinet 112 to control the opening/closing of the sample discarding hole 111.
A second cabinet 113 is arranged on the left side of the bottom of the workstation main body 10, and the control module is arranged in the second cabinet 113.
A third cabinet 114 is arranged at the bottom of the workstation main body 10 between the first cabinet 112 and the second cabinet 113 for placing consumables.
The consumable table comprises a sperm collection dish placing table 70, a test tube placing table 80 and a sample transfer gun head placing table 90, the sperm collection dish placing table 70 is arranged at the end part of the manipulator 102, the test tube placing table 80 is arranged at one side of the sperm collection dish placing table 70, and the sample transfer gun head placing table 90 is arranged at the other side of the sperm collection dish placing table 70.
Referring to fig. 20 to 21, the sperm cell receiving plate 70 includes a first base 701 and a receiving plate 702, the first base 701 is mounted on the bottom of the operation area 101, the receiving plate 702 is detachably mounted on the first base 701, and a plurality of first engaging grooves (not shown) for receiving sperm cells 703 are formed on the receiving plate 702.
The collecting vessel placing plate 702 is arranged in a ladder shape, and a plurality of first clamping grooves are formed in each layer of ladder; or the collecting dish placing plate 702 is tiled, and the first clamping grooves are arranged on the collecting dish placing plate 702 at intervals in multiple rows.
The first base 701 is provided with a first groove (not shown), two sides of the first base 701 are provided with first guide blocks 7011 extending towards the first groove, two sides of the collecting dish placing plate 702 are provided with first sliding grooves 7021, and the first guide blocks 7011 are embedded into the first sliding grooves 7021. This structure can not only be placed the board 702 installation to first base 701 and collection dish and play the guide effect on the one hand, and on the other hand can play and place board 702 and carry out spacing effect to collection dish after board 702 is placed to the collection dish and installs on first base 701.
As shown in fig. 18 and 19, the cuvette placing table 80 includes a second base 801 and a cuvette placing plate 802, the second base 801 is mounted on the bottom of the operation area 101, and the cuvette placing plate 802 is detachably mounted on the second base 801.
The test tube placing plate 802 is arranged in a ladder shape, and each layer of ladder is provided with a plurality of test tube clamping grooves (not shown) for placing test tubes 803.
The test tube placing plate 802 or the second base 801 is provided with a heating mechanism (not shown) inside for heating or keeping a constant temperature of the test tubes placed in the test tube slots.
The second base 801 is provided with a second groove (not shown), the two sides of the second base 801 are provided with second guide blocks 8011 extending towards the direction of the second groove, the two sides of the test tube placing plate 802 are provided with second sliding grooves 8021, and the second guide blocks 8011 are embedded in the second sliding grooves 8021. This structure can place board 802 installation to second base 801 and test tube and play the guide effect on the one hand, and on the other hand can play and place board 802 and carry out spacing effect to the test tube after board 802 is installed on second base 801 to the test tube.
The sample transfer gun head placing platform 90 is installed at the bottom of the operating area 101 and is used for placing a sample transfer gun head 901 for sucking semen.
The sperm collecting vessel placing table 70, the test tube placing table 80 and the specimen transfer gun head placing table 90 can be used independently.
Referring to fig. 16 to 17, the automatic sample feeding device 30 for the semen cup includes a base 301, the base 301 and a sample feeder 302, and a placing chute 303 is disposed in the sample feeder 302 for placing the semen cup 202.
A sample feeding motor (not shown) is arranged in the sample feeder 302 to control the placing chute 303 to rotate, so as to drive the semen cup to move in the placing chute 303.
The sample feeder 302 is provided with a sensor (not shown) facing the placing chute 303 for detecting whether a fine cup is placed in the placing chute 303.
The sensor is an infrared sensor, or a weight sensor, or a distance sensor, etc., as long as the purpose of detecting whether the fine cup is placed in the placing chute 303 can be achieved.
Referring to fig. 14 to 15, the semen console 40 includes a bottom plate 401, and the bottom plate 401 has a test tube accommodating groove 402, a semen cup accommodating groove 403, a sperm collecting vessel accommodating groove 404, a vessel cover accommodating groove 405, a slide accommodating groove 406, and a slide sample dropping groove 407.
The test tube accommodating groove 402 is disposed on one side of the front end portion of the bottom plate 401 for accommodating a test tube 803, and the semen cup accommodating groove 403 is disposed on the opposite side of the front end portion of the bottom plate 401 from the test tube accommodating groove 402 for accommodating a semen cup 202.
The slide accommodating groove 406 is provided on a side of the rear end portion of the base plate 401 for stacking a slide 408, and the slide drop groove 407 is provided on a side of the rear end portion of the base plate 401 opposite to the slide accommodating groove 406 for accommodating the slide 408, so that the robot 102 can make a slide sample.
The sperm collection vessel containing groove 404 is disposed on one side of the middle portion of the bottom plate 401, is located between the sperm cup containing groove 403 and the slide sample dripping groove 407, and is used for placing the sperm collection vessel 703, the vessel cover containing groove 405 is disposed on the middle portion of the bottom plate 401, is located on the opposite side of the sperm collection vessel containing groove 404, is located between the test tube containing groove 402 and the slide containing groove 406, and is used for placing the vessel cover 704 of the sperm collection vessel 703.
The semen automatic analysis device 60 is arranged at the side part of the operation area 101 and is positioned below the semen cup placing platform 20, and is used for reading, analyzing and recording semen information on the slide sample.
The automatic semen analysis device 60 is internally provided with a light source for illumination, a lens for monitoring and recording the analysis steps or the analysis process, and a CDD monitoring module.
The automatic semen analysis device 60 is provided with a jack for inserting a slide sample.
As shown in fig. 11 to 13, the automatic lid opening device 50 includes a box 501, the box 501 is fixed to the workstation main body 10, and a lid opening portion is provided on the box 501.
The case 501 is fixed to an end of the workstation body 10 opposite to the open end of the operating area 101.
The box body 501 is provided with an uncovering area 502, the uncovering area 502 is internally provided with the uncovering part, and the uncovering part is used for uncovering or closing the semen cup 202 and the test tube 803.
The open cover part comprises a first open cover part 503 and a second open cover part 504, the first open cover part 503 is used for opening or closing the cover of the test tube 803, and the second open cover part 504 is used for opening or closing the cover of the semen cup 202.
The first cap opening part 503 comprises a rotating shaft 5031, the top end of the rotating shaft 5031 is embedded in the top wall of the cap opening area 502 or is rotatably installed on the top wall of the cap opening area 502, a cap opening groove 5032 is formed at the bottom end of the rotating shaft 5031 for inserting, clamping and fixing the test tube cap of the test tube 803, and the cap opening or closing process of the test tube 803 is realized along with the clockwise or counterclockwise rotation of the rotating shaft 5031.
The uncapping area 502 is provided with a test tube holding plate 5033 at a position below the first uncapping portion 503, and the test tube holding plate 5033 is provided with an arc-shaped clamping groove 5034 for holding the test tube 803, so that the stability of the test tube 803 during uncapping or uncapping can be effectively improved.
A test tube supporting plate 5035 is arranged below the test tube holding plate 5033 to support the test tube 803, so as to further improve the stability of the test tube 803 when the test tube is opened or closed.
The second cover opening part 504 comprises a rotating rod 5041, the top end of the rotating rod 5041 is embedded into the top wall of the cover opening area 502 or is rotatably installed on the top wall of the cover opening area 502, a clamping jaw 5042 is arranged at the bottom of the rotating rod 5041 and used for clamping the sperm cup 202, and the rotating rod 5041 rotates to realize the cover opening or closing of the sperm cup 202.
As shown in fig. 6 to 10, the robot arm 102 is provided with a gripper mechanism 115, and the gripper mechanism 115 includes a connecting rod 1151, a gripper motor 1152, a first gripper 1153, a second gripper 1154, and an RFID reading module 1155.
Install on the connecting rod 1151 the cleft hand motor 1152, cleft hand motor 1152 one end is connected with first cleft hand 1153, and the other end is connected with second cleft hand 1155, and first cleft hand 1153 all forms with second cleft hand 1154 and snatchs position 1156, install on the lateral wall of cleft hand motor 1152 the RFID reads module 1155, the RFID reads the module orientation snatch position 1156.
The grasping position 1156 of the first gripper 1153 is used for holding the test tube 803, and the grasping position 1156 of the second gripper 1154 is used for holding the sperm cup 202 or the sperm collecting vessel 703.
The top surface of the end of the connecting rod 1151 is provided with a mounting hole 1157, and the manipulator 102 fixes the gripper mechanism 115 to the top of the manipulator 102 through the connection with the mounting hole 1157.
The claw motor 1152 is connected to the end bottom surface of the connection rod 1151 (the opposite surface of the mounting hole 1157).
The other end of the connecting rod 1151 is provided with a CDD monitoring module 1158 for photographing operation steps and operation processes of the monitoring robot 102.
The CDD monitoring module 1158 is a CDD monitoring camera.
RFID chips for the RFID reading module 1155 to read are attached to the semen cup 202, the semen collection dish 703 and the test tube 803.
In the automatic semen processing station provided by the embodiment, the manipulator 102 is arranged in the operation area 101 of the workstation main body 10, and the control module which is arranged in the workstation main body 10 and electrically connected with the manipulator 102 is arranged, so that on one hand, each time node can be accurately recorded by using the control module, and the starting time of each operation step performed by the manipulator 102 is reasonably arranged to achieve the optimal operation processing time; on the other hand can control manipulator 102 through control module, gets into and places platform 20, consumptive material platform, incubator, the automatic appearance device 30 that send of smart cup, semen operation panel 40, automatic uncapping device 50 and semen automatic analysis device 60 in around the smart cup that manipulator 102 distributes and carry out automatic semen processing operation, replaces loaded down with trivial details manual operation, reduces the risk that lowers misoperation when artifical input.
By arranging the RFID reading module 1155 on the gripper mechanism 115 of the manipulator 102, when semen processing is performed, the RFID reading module 1155 reads, identifies and checks RFID chips attached to the semen cup 202, the semen collection dish 703 and the test tube 803, thereby avoiding errors occurring during manual checking.
By arranging a plurality of monitoring cameras 109 in the operation area 101 and arranging the CDD monitoring module 1158 on the gripper mechanism 115 of the manipulator 102, in the semen processing process, each step of semen processing operation of the manipulator 102 can be photographed or archived for later review, so as to avoid the occurrence of wrong operation in the semen processing process.
The semen cup placing table 20, the consumable table, the incubator, the automatic semen cup sample feeding device 30, the semen operating table 40, the automatic lid opening device 50 and the automatic semen analyzing device 60 which are adopted in the embodiment can be used in the embodiment by combining the whole device in the embodiment, and can also be separately and independently used. Or for other devices.
As shown in fig. 22, the semen processing method further comprising the automatic semen processing station comprises the preparation steps before the semen automatic processing:
the first step is as follows: the sperm upstream liquid was placed into the second incubator 104 overnight equilibration the day ahead;
the second step is that: checking whether parameter display of the first incubator 103 and the second incubator 104 and other facilities on the second display screen 106 of the workstation main body 10 is normal;
the third step: placing an appropriate amount of slide 408 into the analytical slide receiving slot 407;
the fourth step: placing an appropriate amount of test tubes 803 into the test tube accommodating grooves 402;
the fifth step: placing a proper amount of the sperm collection vessel 703 into the sperm collection vessel 405;
and a sixth step: two boxes of the sample transfer gun heads 901 are placed on the sample transfer gun head placing table 90.
As shown in fig. 23, the semen processing method comprises the following automated semen processing steps:
step 1: placing the semen cup 202 filled with the semen sample into a placing chute 303 on a sample feeder 302 of the automatic semen cup sample feeder 30, detecting that the semen cup 202 is placed in the placing chute 303 by a sensor on the sample feeder 302, driving the placing chute 303 to rotate by a sample feeding motor, and rotating the semen cup 202 to the tail end of the placing chute;
step 2: the control module senses that a semen cup 202 is placed in the automatic semen cup sample feeding device 30, the manipulator 102 transfers the semen cup 202 to the semen cup placing table 20, and the RFID information, time and placing position of the semen cup 202 are recorded;
and step 3: after the control module judges that the semen cup 202 is placed on the semen cup placing table 20 for 30min according to the stored information (the time can be set by self), the manipulator 102 transfers the semen cup 202 from the semen cup placing table 20 to the automatic cover opening device 50 for opening the cover, and then transfers the semen cup 202 to the semen cup accommodating groove 403 of the semen operation table 40;
and 4, step 4: the manipulator 102 transfers the sperm collection vessel 703 attached with the same information RFID chip from the sperm collection vessel placing table 70 of the consumable table to the sperm collection vessel accommodating groove 404 of the semen operation table 40, and opens the dish cover 704 to be placed in the dish cover accommodating groove 405 of the semen operation table 40;
and 5: the manipulator 102 obtains a clean sample transfer gun head 901 on the sample transfer gun head placing platform 90, sucks a certain amount of semen from the semen cup 202 in the semen cup accommodating groove 403, and injects the semen into the bottom of the collecting hole of the semen collecting vessel 703;
step 6: the manipulator 102 sucks a small amount of semen in the semen cup 202 again and injects the semen into the slide 408 in the slide dripping groove 407 to prepare a slide sample, and the slide sample is inserted into the semen automatic analysis device 60 for reading, analysis and recording;
and 7: the manipulator 102 throws the used sample-moving gun head 901 into the garbage collection box 110 below the sample-throwing hole 111 through the sample-throwing hole 111; continuously obtaining a clean sample transferring gun head 901 on the sample transferring gun head placing platform 90, entering the second incubator 104 through the sampling hole 1041 on the top wall of the second incubator 104, and sucking quantitative liquid to inject into the collecting hole of the sperm collecting vessel 703;
and 8: the manipulator 102 covers the dish cover 704 back onto the sperm collection dish 703, transfers the sperm collection dish 703 to the tower turntable 1032 in the first incubator 103, and records the standing time;
and step 9: the manipulator 102 transfers the semen cup 202 on the semen operation table 40 to the automatic cover opening device 50 for closing the cover, and throws the semen cup into the garbage collection box 110 below the sample losing hole 111 from the sample losing hole 111, and takes out the slide sample from the automatic semen analysis device 60, and throws the slide sample into the garbage collection box 110 below the sample losing hole 111 from the sample losing hole 111;
step 10: after the control module judges that the sperm collection vessel 703 is placed in the first incubator 103 for 60min (the time can be set by itself) according to the stored information, the manipulator 102 takes the sperm collection vessel 703 out of the first incubator 103 and transfers the sperm collection vessel 703 to the sperm collection vessel accommodating groove 404 of the semen console 40;
step 11: the manipulator 102 transfers the test tube 803 attached with the same information RFID chip from the test tube accommodating groove 403 to the automatic cap opening device 50 for cap opening and then transfers the test tube 803 to the test tube accommodating groove 402 of the semen operating platform 40;
step 12: the manipulator 102 opens the capsule 704 and places the capsule into the capsule accommodating groove 405 of the semen operating platform 40, then the sample transfer gun head placing platform 90 obtains a clean sample transfer gun head 901, sucks quantitative upstream liquid of the sperms from the sperm collecting capsule 703 and transfers the upstream liquid into the test tube 803;
step 13, the manipulator 102 sucks a trace amount of upstream liquid of the sperms in the test tube 803 again and injects the upstream liquid into the slide 408 in the slide dropping groove 407 to prepare a slide sample, and the slide sample is inserted into the automatic semen analyzing device 60 to be read, analyzed and recorded; the manipulator 102 throws the used sample transferring gun head 901 into the garbage collection box 110 below the sample throwing hole 111 through the sample throwing hole 111;
step 14: the manipulator 102 transfers the test tube 803 to the automatic cap opening device 50 for cap closing and puts it back into the test tube accommodating groove 402;
step 15: the manipulator 102 covers the dish cover 704 back to the sperm collecting dish 703 and throws the dish cover from the sample throwing hole 111 into the garbage collection box 110 below the sample throwing hole 111; and the slide specimen is taken out of the automatic semen analyzing apparatus 60 and dropped from the discard hole 111 into the trash receptacle 110 below the discard hole 111.
The clean sample transfer gun head 901 is specifically in a relatively clean state (sterile clean state) as long as the semen is not affected when the semen is sucked, and the absolute clean state is not required to be realized.
It should be noted that the above working steps are operation steps of a single semen sample, and actually, a plurality of semen samples can be alternately operated in sequence through the identification of the RFID chip information and the judgment of each time node by the control module; all operations are recorded by the monitoring camera 109 in the whole process, especially the RFID information check and the photographing and keeping of files are needed in each sample transfer of the semen console 40, and related information is also displayed on the first display screen 105 at the side of the workstation body 10.
The above embodiments are not intended to be exhaustive list of the present invention, and there may be many other embodiments not listed. Any replacement and improvement made on the basis of not violating the concept of the utility model belong to the protection scope of the utility model.