CN217499235U - Probiotics multi-strain mixed fermentation system for genital tract - Google Patents
Probiotics multi-strain mixed fermentation system for genital tract Download PDFInfo
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- CN217499235U CN217499235U CN202221457954.1U CN202221457954U CN217499235U CN 217499235 U CN217499235 U CN 217499235U CN 202221457954 U CN202221457954 U CN 202221457954U CN 217499235 U CN217499235 U CN 217499235U
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- 238000000855 fermentation Methods 0.000 title claims abstract description 85
- 230000004151 fermentation Effects 0.000 title claims abstract description 85
- 210000005000 reproductive tract Anatomy 0.000 title claims abstract description 21
- 239000006041 probiotic Substances 0.000 title claims description 14
- 235000018291 probiotics Nutrition 0.000 title claims description 14
- 238000011534 incubation Methods 0.000 claims abstract description 39
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 37
- 230000001580 bacterial effect Effects 0.000 claims abstract description 16
- 238000003756 stirring Methods 0.000 claims abstract description 16
- 238000002156 mixing Methods 0.000 claims description 18
- 239000011229 interlayer Substances 0.000 claims description 9
- 230000000529 probiotic effect Effects 0.000 claims description 7
- 239000010410 layer Substances 0.000 claims description 3
- 241000233866 Fungi Species 0.000 abstract description 9
- 230000008901 benefit Effects 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 description 9
- 239000001963 growth medium Substances 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000012258 culturing Methods 0.000 description 3
- 238000009630 liquid culture Methods 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 206010061218 Inflammation Diseases 0.000 description 2
- 230000008485 antagonism Effects 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 229940001882 lactobacillus reuteri Drugs 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 208000004926 Bacterial Vaginosis Diseases 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 208000036649 Dysbacteriosis Diseases 0.000 description 1
- 208000027244 Dysbiosis Diseases 0.000 description 1
- 241000186604 Lactobacillus reuteri Species 0.000 description 1
- 241000218588 Lactobacillus rhamnosus Species 0.000 description 1
- 208000007074 Trichomonas Vaginitis Diseases 0.000 description 1
- 208000025206 Trichomonas vaginitis urogenital infection Diseases 0.000 description 1
- 201000008100 Vaginitis Diseases 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000007140 dysbiosis Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a genital tract is with many bacterials of living fungus mixed fermentation system of benefit, including steam conduit, compressed air pipeline, constant temperature circulating water pipeline, mixed incubation jar and a plurality of fermentation cylinder, steam conduit one end be connected with the compressed air pipeline, constant temperature circulating water pipeline include constant temperature circulating water inlet pipe and constant temperature circulating water return water pipeline, mixed incubation jar be equipped with agitator motor respectively with the fermentation cylinder top, the agitator motor output stretch into jar internal portion and connect and be equipped with the stirring screw. The utility model discloses a genital tract is with many bacterials of living fungus mixed fermentation system can high-efficiently realize many bacterials and mix the fermentation as required, mixes the bacterial strain and stabilizes and effectual.
Description
Technical Field
The utility model relates to a living fungus processing production technical field specifically indicates a reproduction way is with many strains of living fungus mixed fermentation system of living fungus of benefit.
Background
The health of the genital tract flora is the key to keeping the genital tract flora free from pathogenic bacteria, for example, the infection of female vagina inflammation is mainly caused by the dysbacteriosis of the genital tract flora and pathogenic bacteria infection. The healthy genital tract flora is complex and diverse, and diseases of the genital tract such as bacterial vaginitis and trichomonas vaginitis can be treated by probiotics with bacteriostatic or inflammation eliminating functions. The research and application of probiotics in the aspects of preventing and treating genital tract bacterial/viral infection, inflammation and the like are relatively deep, and multiple strains are generally adopted for combined use.
At present, a multi-strain combined preparation is usually prepared in a mode of obtaining single-strain freeze-dried powder and then mixing and compounding, but biological antagonism exists among different strains, and a certain adaptation process is needed in the early stage of co-growth. The more effective multi-strain combined preparation is to perform multi-strain mixed fermentation in the fermentation process, but in the actual multi-strain mixed fermentation process, the propagation rate and the adaptability of different strains are different, only individual strains become dominant strains when the multi-strain mixed strains are inoculated into a fermentation tank for fermentation, and other strains hardly grow or have lower proportion.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model is to solve above problem, provide a reproduction way with many bacterials of living fungus mixed fermentation system.
In order to solve the technical problem, the utility model provides a technical scheme does: a multi-strain mixed fermentation system of probiotics for genital tracts comprises a steam pipeline, a compressed air pipeline, a constant-temperature circulating water pipeline, a mixed incubation tank and a plurality of fermentation tanks, wherein one end of the steam pipeline is connected with the compressed air pipeline, the constant-temperature circulating water pipeline comprises a constant-temperature circulating water inlet pipeline and a constant-temperature circulating water return pipeline, stirring motors are respectively arranged above the mixed incubation tank and the fermentation tanks, the output ends of the stirring motors stretch into the tank body and are connected with stirring propellers, the mixed incubation tank and the side wall above the fermentation tanks are respectively connected with a tank body interlayer water outlet pipeline which is communicated with each other, the upper ends of the tank body interlayer water outlet pipelines are connected with the constant-temperature circulating water return pipeline, one sides above the mixed incubation tank and the upper sides above the fermentation tanks are respectively connected with a pipeline which is provided with steam/compressed air to enter the tank body, and the upper ends of the steam/compressed air to enter the tank body inner pipeline are connected with the compressed air pipeline, the mixed incubation tank and the fermentation tank top one side between be connected and be equipped with exhaust duct, the fermentation tank lower extreme between connect and be equipped with drain pipe one, the drain pipe other end and mixed incubation tank upper end be connected, drain pipe lateral wall be connected with steam conduit, the mixed incubation tank lower extreme connect and be equipped with drain pipe two, constant temperature circulating water inlet pipe lower extreme be equipped with and stretch into the constant temperature circulating water inlet tank body intermediate layer pipeline that mixes incubation tank and a plurality of fermentation tank below lateral wall respectively.
As an improvement, the side walls of the pipelines are respectively provided with a valve.
As an improvement, the stirring propeller comprises a vertical rod and a plurality of blades arranged on the side wall of the vertical rod, and the number of the blades is three.
As a modification, the number of the fermentation tanks is at least two.
Compared with the prior art, the utility model the advantage lie in: the utility model discloses a genital tract is with many bacterial strains of living fungus mixed fermentation system of living fungus cultivates each bacterial strain of living fungus respectively in the fermentation cylinder earlier, makes it can obtain good growth, and the jar is hatched in the mixture of rethread, makes each bacterial strain of many bacterial strains joint preparation that obtains grow well, increases the adaptability between each bacterial strain, increases the utility of product.
Drawings
Fig. 1 is a schematic structural diagram of the probiotic multi-strain mixed fermentation system for the genital tract of the utility model.
Fig. 2 is a schematic structural diagram of a fermentation tank of the probiotic multi-strain mixed fermentation system for the genital tract of the utility model.
As shown in the figure: 1. a mixing incubation tank; 2. a fermentation tank; 3. a stirring motor; 4. a stirring propeller; 5. a water outlet pipeline of the interlayer of the tank body; 6. the steam/compressed air enters a pipeline inside the tank body; 7. a steam line; 8. a compressed air conduit; 9. a constant temperature circulating water inlet pipeline; 10. a constant-temperature circulating water return pipeline; 11. an exhaust duct; 12. a liquid discharge pipeline I; 13. a second liquid discharge pipeline; 14. circulating water at constant temperature enters the interlayer pipeline of the tank body; 15. and (4) a valve.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
With reference to the attached drawings 1 and 2, the probiotic multi-strain mixed fermentation system for the genital tract comprises a steam pipeline 7, a compressed air pipeline 8, a constant-temperature circulating water pipeline, a mixed incubation tank 1 and a plurality of fermentation tanks 2, wherein one end of the steam pipeline 7 is connected with the compressed air pipeline 8, the constant-temperature circulating water pipeline comprises a constant-temperature circulating water inlet pipeline 9 and a constant-temperature circulating water return pipeline 10, the number of the fermentation tanks 2 is at least two, the fermentation tanks can be cultured by increasing the number of the fermentation tanks according to the number of strains to be cultured, stirring motors 3 are respectively arranged above the mixed incubation tank 1 and the fermentation tanks 2, the output ends of the stirring motors 3 extend into the tank body and are connected with stirring propellers 4, the side walls above the mixed incubation tank 1 and the fermentation tanks 2 are respectively connected with communicated tank interlayer water outlet pipelines 5, the upper ends of the tank interlayer water outlet pipelines 5 are connected with the constant-temperature circulating water return pipeline 10, one side above the mixed incubation tank 1 and the fermentation tank 2 is respectively connected with a steam/compressed air inlet tank body internal pipeline 6, the upper end of the steam/compressed air inlet tank body internal pipeline 6 is connected with a compressed air pipeline 8, an exhaust pipeline 11 is connected between the mixed incubation tank 1 and one side above the fermentation tank 2, a drainage pipeline I12 is connected between the lower end of the fermentation tank 2, the other end of the drainage pipeline I12 is connected with the upper end of the mixed incubation tank 1, the side wall of the drainage pipeline I12 is connected with a steam pipeline 7, the lower end of the mixed incubation tank 1 is connected with a drainage pipeline II 13, the lower end of the constant temperature circulating water inlet pipeline 9 is provided with a constant temperature circulating water inlet tank body interlayer pipeline 14 which respectively extends into the side walls below the mixed incubation tank 1 and the plurality of fermentation tanks 2, the side walls of the pipelines are respectively provided with a valve 15, used for controlling the independent culture and the mixed culture of the strains.
Stirring screw 4 include vertical pole and set up a plurality of paddles at vertical pole lateral wall, the quantity of paddle be three, make the strain in mixing incubation jar 1 and the fermentation cylinder 2 cultivate well.
The utility model discloses when the concrete implementation, a mixed fermentation system for many bacterial strains of genital tract probiotics, cultivate different bacterial strains in fermentation cylinder 2 respectively, each valve 15 is in the closed condition, 1.5h before each bacterial strain fermentation finishes, open respectively and mix exhaust duct 11 of incubation jar 1, steam conduit 7 connected with drain line 12, drain line 15 on drain line one 12 and the drain line two 13, and carry out high temperature (115 ℃) sterilization 0.5h with drain line one 12, mix incubation jar 1, drain line two 13 after, close drain line two 13 in proper order, mix exhaust duct 11 of incubation jar 1, drain line one 12 and steam conduit 7's valve 15, open compressed air pipe 8's valve 15, regulate and control the valve 15 that the constant temperature circulating water advances on jar body intermediate layer pipeline 14, make fermentation cylinder 2 be in the malleation state, pressure is in the 0.5-1.0Mpa within range, opening a valve 15 on a drainage pipeline I12, performing a mixing rotary tank to enable fermentation liquor to enter a mixing incubation tank 1, adjusting and controlling the valve 15 on an exhaust pipeline 11 connected with the mixing incubation tank 1 to enable the interior of the mixing incubation tank 1 to be in a positive pressure state, wherein the pressure range is 0.2-0.4Mpa until a fermentation tank 2 is emptied, and closing the valve 15, wherein during the mixing rotary tank, the fermentation liquor with a target mixing proportion can be obtained by adjusting the pressure in the fermentation tank 2 and the valve 15 on the drainage pipeline I12, the fermentation liquor is further fermented in the mixing incubation tank 1, after the mixing incubation fermentation is completed, the valve 15 on a drainage pipeline II 13 is opened, the fermentation liquor is taken out, and strains are harvested through centrifugation, so that the obtained multiple strains meet requirements better.
Meanwhile, the number of the fermentation tanks 2 is at least two, and the number of the fermentation tanks can be increased according to the number of the strains to be cultured for culturing; the stirring propeller 4 comprises a vertical rod and a plurality of blades arranged on the side wall of the vertical rod, and the number of the blades is three, so that the strains in the mixed incubation tank 1 and the fermentation tank 2 are well cultured.
In order to verify the effect of the multi-strain mixed fermentation system, two strains of probiotics for reproductive tract, namely lactobacillus rhamnosus GR-1 and lactobacillus reuteri RC-14 are selected.
Streaking two strains in MRS culture medium, selecting single colony to 10ml MRS liquid culture medium, culturing at 37 deg.C for 12 hr, and adjusting OD of two strains with liquid MRS culture medium 600 And (5) obtaining seed liquid with the same viable bacteria amount of the two strains. The control group was: respectively taking two strain seed solutions, inoculating the two strain seed solutions to an MRS liquid culture medium according to the inoculation amount of 5%, culturing for 12-16 hours at 37 ℃ until the logarithmic phase of strain fermentation, and taking end-point fermentation liquid as a sample A.
The experimental groups were: by using the multi-strain mixed fermentation system, two strain seed liquids are respectively taken and respectively inoculated to an MRS liquid culture medium according to the inoculation amount of 5%, the two strain seed liquids are cultured for 11 to 15 hours at 37 ℃ until the logarithmic phase of strain fermentation, and then the two strain fermentation liquids are mixed and incubated for 1 hour to obtain a mixed incubation fermentation liquid as a sample B.
Viable bacteria count and metagenome sequencing are respectively carried out on the fermentation liquor sample A and the sample B, and the results are respectively shown in tables 1 and 2. The number of viable bacteria in the mixed incubation fermentation broth of the experimental group is higher than that of the viable bacteria in the control group, the proportional structure of each strain is close to 1:1, and the ratio difference of two strains in the final fermentation broth is larger after the strains of the experimental group are inoculated and fermented according to the ratio of 1: 1. Therefore, the effect of the multi-strain mixed fermentation system is expected, and the system is more suitable for mixed fermentation of strains with inconsistent growth rate or mutual antagonism.
TABLE 1 fermentation broth sample viable count
| Bacterial strain | Fermentation broth A | Fermentation broth B |
| Viable count (CFU/mL) | 4.2×10 9 | 6.7×10 9 |
TABLE 2 fermentation broth sample metagenome sequencing results (species level)
| Strain | Fermentation broth A | Fermentation broth B |
| S_Lactobacillus reuteri | 6.73％ | 43.72％ |
| S_Lactobacillus rhamnosus | 87.15％ | 55.20％ |
| S_Others | 6.12％ | 1.08％ |
The present invention and the embodiments thereof have been described above, but the description is not limited thereto, and the embodiment shown in the drawings is only one of the embodiments of the present invention, and the actual structure is not limited thereto. In summary, those skilled in the art should understand that they should not be limited to the embodiments described above, and that they can design the similar structure and embodiments without departing from the spirit of the invention.
Claims (4)
1. The utility model provides a genital tract is with many bacterials of probiotic mixed fermentation system, includes steam conduit (7), compressed air pipeline (8), constant temperature circulating water pipeline, mixes incubation tank (1) and a plurality of fermentation cylinder (2), its characterized in that: one end of the steam pipeline (7) is connected with the compressed air pipeline (8), the constant-temperature circulating water pipeline comprises a constant-temperature circulating water inlet pipeline (9) and a constant-temperature circulating water return pipeline (10), stirring motors (3) are respectively arranged above the mixing incubation tank (1) and the fermentation tank (2), the output end of each stirring motor (3) stretches into the tank body and is connected with a stirring propeller (4), the side walls above the mixing incubation tank (1) and the fermentation tank (2) are respectively connected with a communicated tank body interlayer water outlet pipeline (5), the upper end of the tank body interlayer water outlet pipeline (5) is connected with the constant-temperature circulating water return pipeline (10), one side above the mixing incubation tank (1) and the fermentation tank (2) is respectively connected with a steam/compressed air inlet tank body inner pipeline (6), and the upper end of the steam/compressed air inlet tank body inner pipeline (6) is connected with the compressed air pipeline (8), the mixing incubation tank (1) and the fermentation tank (2) top one side between connect and be equipped with exhaust duct (11), fermentation tank (2) lower extreme between connect and be equipped with drain pipe one (12), drain pipe one (12) other end and mixing incubation tank (1) upper end be connected, drain pipe one (12) lateral wall be connected with steam conduit (7), mixing incubation tank (1) lower extreme connect and be equipped with drain pipe two (13), constant temperature circulating water inlet pipe (9) lower extreme be equipped with and stretch into respectively that the mixing incubation tank (1) and the constant temperature circulating water of a plurality of fermentation tank (2) below lateral wall enter jar body intermediate layer pipeline (14).
2. The probiotic multi-strain mixed fermentation system for the genital tract according to claim 1, wherein: the side walls of the pipelines are respectively provided with a valve (15).
3. The probiotic multi-strain mixed fermentation system for the genital tract according to claim 1, wherein: the stirring propeller (4) comprises a vertical rod and a plurality of blades arranged on the side wall of the vertical rod, and the number of the blades is three.
4. The probiotic multi-strain mixed fermentation system for the genital tract according to claim 1, wherein: the number of the fermentation tanks (2) is at least two.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202221457954.1U CN217499235U (en) | 2022-06-13 | 2022-06-13 | Probiotics multi-strain mixed fermentation system for genital tract |
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| CN202221457954.1U CN217499235U (en) | 2022-06-13 | 2022-06-13 | Probiotics multi-strain mixed fermentation system for genital tract |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN120555151A (en) * | 2025-07-30 | 2025-08-29 | 成都圣恩生物科技股份有限公司 | Multi-category vegetable circulation fermentation system and fermentation method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN120555151A (en) * | 2025-07-30 | 2025-08-29 | 成都圣恩生物科技股份有限公司 | Multi-category vegetable circulation fermentation system and fermentation method |
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