CN217385316U - Uric acid detection test paper - Google Patents
Uric acid detection test paper Download PDFInfo
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- CN217385316U CN217385316U CN202221208387.6U CN202221208387U CN217385316U CN 217385316 U CN217385316 U CN 217385316U CN 202221208387 U CN202221208387 U CN 202221208387U CN 217385316 U CN217385316 U CN 217385316U
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- 238000012360 testing method Methods 0.000 title claims abstract description 84
- 238000001514 detection method Methods 0.000 title claims abstract description 47
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 title claims abstract description 26
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 title claims abstract description 26
- 229940116269 uric acid Drugs 0.000 title claims abstract description 26
- 239000010410 layer Substances 0.000 claims abstract description 71
- 239000008280 blood Substances 0.000 claims abstract description 44
- 210000004369 blood Anatomy 0.000 claims abstract description 44
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 27
- 239000000758 substrate Substances 0.000 claims abstract description 26
- 239000011229 interlayer Substances 0.000 claims abstract description 18
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 27
- 229910052799 carbon Inorganic materials 0.000 claims description 27
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims description 16
- 229910052709 silver Inorganic materials 0.000 claims description 10
- 239000004332 silver Substances 0.000 claims description 10
- 238000009534 blood test Methods 0.000 abstract description 3
- 210000001124 body fluid Anatomy 0.000 abstract 1
- 239000010839 body fluid Substances 0.000 abstract 1
- 238000005192 partition Methods 0.000 description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 6
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 6
- 239000008103 glucose Substances 0.000 description 6
- 239000012790 adhesive layer Substances 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 125000006850 spacer group Chemical group 0.000 description 5
- 239000007789 gas Substances 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 3
- 235000012000 cholesterol Nutrition 0.000 description 3
- 239000011248 coating agent Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 229940088598 enzyme Drugs 0.000 description 3
- 229920003063 hydroxymethyl cellulose Polymers 0.000 description 3
- 229940031574 hydroxymethyl cellulose Drugs 0.000 description 3
- 229910052708 sodium Inorganic materials 0.000 description 3
- 239000011734 sodium Substances 0.000 description 3
- 239000012445 acidic reagent Substances 0.000 description 2
- 230000009977 dual effect Effects 0.000 description 2
- -1 potassium ferricyanide Chemical compound 0.000 description 2
- YRNWIFYIFSBPAU-UHFFFAOYSA-N 4-[4-(dimethylamino)phenyl]-n,n-dimethylaniline Chemical compound C1=CC(N(C)C)=CC=C1C1=CC=C(N(C)C)C=C1 YRNWIFYIFSBPAU-UHFFFAOYSA-N 0.000 description 1
- 108010089254 Cholesterol oxidase Proteins 0.000 description 1
- 229910000604 Ferrochrome Inorganic materials 0.000 description 1
- 108010050375 Glucose 1-Dehydrogenase Proteins 0.000 description 1
- 201000005569 Gout Diseases 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 201000001431 Hyperuricemia Diseases 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 108010055297 Sterol Esterase Proteins 0.000 description 1
- 102000000019 Sterol Esterase Human genes 0.000 description 1
- 108010092464 Urate Oxidase Proteins 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 229910021538 borax Inorganic materials 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- UQGFMSUEHSUPRD-UHFFFAOYSA-N disodium;3,7-dioxido-2,4,6,8,9-pentaoxa-1,3,5,7-tetraborabicyclo[3.3.1]nonane Chemical compound [Na+].[Na+].O1B([O-])OB2OB([O-])OB1O2 UQGFMSUEHSUPRD-UHFFFAOYSA-N 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- XGZVUEUWXADBQD-UHFFFAOYSA-L lithium carbonate Chemical compound [Li+].[Li+].[O-]C([O-])=O XGZVUEUWXADBQD-UHFFFAOYSA-L 0.000 description 1
- 229910052808 lithium carbonate Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- ZPIRTVJRHUMMOI-UHFFFAOYSA-N octoxybenzene Chemical compound CCCCCCCCOC1=CC=CC=C1 ZPIRTVJRHUMMOI-UHFFFAOYSA-N 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- NRHMKIHPTBHXPF-TUJRSCDTSA-M sodium cholate Chemical compound [Na+].C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC([O-])=O)C)[C@@]2(C)[C@@H](O)C1 NRHMKIHPTBHXPF-TUJRSCDTSA-M 0.000 description 1
- 235000010339 sodium tetraborate Nutrition 0.000 description 1
- 239000004328 sodium tetraborate Substances 0.000 description 1
- 229940005267 urate oxidase Drugs 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The application relates to the technical field of uric acid detects, especially relates to a uric acid test paper, and it includes substrate, carbon-layer electrode, well interlayer, adhesion layer and upper cover layer, the one end of substrate is equipped with the body fluid inflow end of examining, the other end of substrate is equipped with circuit loop output, be equipped with the wire on the substrate, be equipped with the detect reagent who is used for the detection on the well interlayer, the carbon-layer electrode is including full blood electrode, working electrode and contrast electrode. The full blood test of the test paper to the detected body can be realized through the arrangement of the contrast electrode, the working electrode and the full blood electrode, and the test accuracy of the test paper is improved through the arrangement of the full blood electrode.
Description
Technical Field
The application relates to the technical field of electrode-method blood glucose testing, in particular to uric acid detection test paper.
Background
At present, the number of hyperuricemia patients in China reaches 1.2 hundred million, wherein the number of gout patients exceeds 7500 million, and the annual growth rate is rapidly increased by 9.7 percent every year, thus showing the trend of youthfulness. Therefore, the regular detection of the blood fat concentration in vivo is as important as the detection of blood sugar or uric acid, and no matter in clinical use or home health care of hospitals, the multifunctional tester is matched with the blood sugar, uric acid and blood fat test pieces for use, so that the accuracy and convenience are very needed
The prior art provides a blood sugar test paper, including the substrate, the one end of substrate is equipped with the siphon passageway, and the other end is equipped with the return circuit output terminal that is used for being connected with the blood glucose meter, be equipped with working electrode, reference electrode and counter electrode between siphon passageway and the return circuit output terminal, the last enzyme coating that covers of working electrode, the one end and the enzyme coating of siphon passageway connect, the other end is equipped with the blood sampling mouth, and the enzyme coating top covers there is the hydrophilic membrane of one deck.
The technology has the same structure as the conventional blood glucose test paper, a hydrophilic layer is provided for one more process, the design of filling the identification electrode is lacked, the measurement fails when the blood volume is too small, the alarm function is not provided, and the siphon channel on one side causes troubles to users with different habits of left and right hands.
SUMMERY OF THE UTILITY MODEL
In order to improve the test accuracy of test paper, this application provides a uric acid test paper.
The application provides a uric acid test paper adopts following technical scheme:
a uric acid detection test paper comprises a substrate, a carbon layer electrode, a middle interlayer, an adhesive layer and an upper cover layer, wherein one end of the substrate is provided with a sample inflow end, the other end of the substrate is provided with a circuit loop output end, the substrate is provided with a lead, the middle interlayer is provided with a detection reagent for detection, and the carbon layer electrode comprises a full blood electrode, a working electrode and a comparison electrode.
By adopting the technical scheme, the sample flows in from the sample inflow end of the substrate, sequentially passes through the working electrode, the contrast electrode and the full blood electrode on the carbon layer electrode, and then is input into the tester from the output end of the circuit loop; the full blood electrode is arranged on the carbon layer electrode, so that when the blood volume is too small, prompt can be performed, the situation of measurement failure when the blood volume is too small is reduced, full blood test of the test paper on a sample can be realized through the arrangement of the contrast electrode, the working electrode and the full blood electrode, the multifunctional application of the test paper is realized, the detection situation of insufficient blood volume is reduced through the arrangement of the full blood electrode, and the test accuracy of the test paper is improved.
Optionally, the detection reagent is arranged on one side of the middle partition layer close to the inflow end of the sample.
By adopting the technical scheme, the detection reagent is arranged on the side, close to the inflow end of the sample, of the middle partition layer, the speed of the sample flowing into the detection reagent can be improved, the detection efficiency of the test paper is improved, the distance between the detection reagent and the inflow end of the sample is shortened, the sample can immediately react with the detection reagent when flowing in, and the detection accuracy is improved.
Optionally, the lead is a silver lead, and the silver lead is short-circuited or open-circuited with three electrode contacts of the carbon layer electrode to serve as a detection identifier.
Through adopting above-mentioned technical scheme, silver-colored wire and carbon-layer electrode mutually support, can guarantee that electric current flows through working electrode under the stable condition of electric potential to the realization is to the detection of examining the body, and silver-colored wire and carbon-layer electrode short circuit each other or when opening circuit, as the detection sign of examining the body, can carry out dual guarantee to detecting, improve the detection accuracy of test paper.
Optionally, the contrast electrode and the working electrode loop provide a sample triggering function, and the contrast electrode and the full blood electrode loop provide a full blood identification function.
By adopting the technical scheme, the comparison electrode and the working electrode form a short circuit or an open circuit firstly, the current triggers the detection function of the sample after flowing through, and then the current passing through the comparison electrode and the full blood electrode identifies the blood volume so as to ensure that the blood volume meets the detection.
Optionally, the middle interlayer is provided with a second vent hole or a third vent hole.
By adopting the technical scheme, the second vent hole or the third vent hole is formed in the middle partition layer, so that gas generated when the test body flows through the middle partition layer can be discharged through the vent holes, the test body can flow on the test paper more smoothly, and the inspection error caused by gas in the test process can be reduced; the reagent is arranged on the middle interlayer and reacts on the middle interlayer, and the exhaust hole is arranged on the middle interlayer, so that the reaction accuracy can be improved, and the test accuracy can be improved.
Optionally, the upper cover layer is provided with a first vent hole.
By adopting the technical scheme, the upper cover layer is provided with the first vent hole and is positioned at the uppermost part of the test paper, bubbles can be generated in the bonding or testing process of the test paper because the test paper comprises a plurality of layers of different material layers, and the first vent hole is arranged on the upper cover layer, so that the bubbles generated in the bonding or testing process can be removed.
Optionally, the carbon layer electrode, the middle spacer layer, the adhesion layer and the upper cover layer are sequentially overlapped on the substrate.
By adopting the technical scheme, the carbon layer electrode, the middle interlayer, the adhesion layer and the upper cover layer are overlapped on the substrate, so that the volume of the test paper can be saved, the space occupation condition of the test paper is reduced, and the convenience in use and carrying of the test paper is improved; and reduces the production cost.
Optionally, the carbon layer electrode and the silver wire are provided with identification pins corresponding in number and position, so that the carbon layer and the silver wire are simultaneously short-circuited or opened.
Through adopting above-mentioned technical scheme, through set up quantity and the corresponding foot position of discerning in position on carbon-layer and silver-colored wire, be favorable to realizing that carbon-layer and silver-colored wire realize short circuit or open circuit simultaneously to satisfy the effective detection of test paper to the physical examination.
In summary, the present application includes at least one of the following beneficial technical effects:
1. the full blood test of the test paper on a test body can be realized by arranging the contrast electrode, the working electrode and the full blood electrode, the multifunctional application of the test paper is realized, the detection condition of insufficient blood volume is reduced by arranging the full blood electrode, and the test accuracy of the test paper is improved;
2. the detection reagent is arranged on one side of the middle partition layer close to the inflow end of the test body, so that the speed of the test body flowing into the detection reagent can be increased, and the detection efficiency of the test paper is improved;
3. through the dual arrangement of the sample trigger circuit and the full blood identification circuit, the misjudgment rate of the detection index is reduced, and therefore the accuracy of the test paper is improved.
Drawings
FIG. 1 is a schematic view of the overall structure of embodiment 1;
FIG. 2 is an exploded view of example 1;
FIG. 3 is an exploded view of example 2;
FIG. 4 is an exploded view of embodiment 3.
Description of reference numerals: 1. a substrate; 2. a wire; 3. a carbon layer electrode; 31. a contrast electrode; 32. a working electrode; 33. a full blood electrode; 4. a middle barrier layer; 41. a second vent hole; 42. a third exhaust hole; 5. a detection reagent; 6. an adhesive layer; 61. a sample inflow port; 7. an upper cover layer; 71. a first exhaust hole.
Detailed Description
The present application is described in further detail below with reference to the accompanying drawings.
Example 1
The embodiment of the application discloses uric acid detection test paper. Referring to fig. 1 and 2, a uric acid test paper comprises a substrate 1 provided with a lead 2, a carbon layer electrode 3, a middle partition layer 4 provided with a detection reagent 5, an adhesive layer 6 and an upper cover layer 7, specifically, the lead 2 is a silver lead 2, a sample inflow end is arranged at one end of the substrate 1, a circuit loop output end for the carbon layer electrode 3 to work is arranged at the other end of the substrate 1, the silver lead 2 and the carbon layer electrode 3 comprise two corresponding identification pins and a grounding part, a reaction area is arranged on the middle partition layer 4, and the detection reagent 5 is identified by short circuit or open circuit of three circuit contact points of the silver lead 2 and the carbon layer electrode 3.
Referring to fig. 1 and 2, the detection reagent 5 is a blood glucose reagent for detecting blood glucose, a uric acid reagent for detecting uric acid, or a total cholesterol reagent for detecting total cholesterol, the detection reagent 5 is disposed at one end of the middle spacer layer 4 close to the identification foot of the carbon layer electrode 3, and specifically, the blood glucose reagent mainly comprises sodium hydroxymethyl cellulose, glucose dehydrogenase, potassium ferricyanide, sodium hydrogen phosphate buffer and polyethylene glycol octyl phenyl ether; the main components of the uric acid reagent are sodium hydroxymethyl cellulose, urate oxidase, ferrochrome salt, lithium carbonate, sodium tetraborate and potassium dihydrogen phosphate; the total cholesterol reagent mainly comprises sodium hydroxymethyl cellulose, sodium cholate, cholesterol esterase, cholesterol oxidase, horseradish peroxidase, tetramethyl benzidine and potassium ferricyanide.
Referring to fig. 1 and 2, the carbon layer electrode 3 includes a full blood electrode 33, a working electrode 32 and a contrast electrode 31, in this embodiment, the contrast electrode 31 and the working electrode 32 form a loop to provide a sample triggering function, the contrast electrode 31 and the full blood electrode 33 form a loop to provide a full blood identification function, and detection is performed when the blood volume on the test paper is sufficient; further, when the test paper is detected, after the currents of the working electrode 32 and the comparison electrode 31 on the test paper are collected, the currents are transmitted to a detector for detection to amplify and convert signals, the signals are converted into concentrations and then transmitted to a display screen of the detector, and a final measurement result is displayed.
Referring to fig. 1 and 2, the substrate 1, the carbon layer electrode 3, the spacer 4, the adhesion layer 6 and the upper cover layer 7 are sequentially overlapped and connected, and have substantially the same shape; in this embodiment, a rectangular specimen inlet 61 is provided at one end of the spacer layer 4 and the adhesive layer 6 away from the carbon layer electrode 3 at which the foot position is recognized, and the size and shape of the specimen inlet 61 of the spacer layer 4 and the adhesive layer 6 are matched.
Referring to fig. 1 and 2, in the present embodiment, the upper cover layer 7 has a first exhaust hole 71 opened at one end close to the sample inlet 61, and specifically, the first exhaust hole 71 has a substantially circular shape, is smaller in area than the sample inlet 61, and communicates with the sample inlet 61.
The implementation principle of the embodiment 1 is as follows: the test paper flows into the test paper through the test inlet 61, and sequentially passes through a loop formed by the silver lead 2 and the carbon layer electrode 3 on the substrate 1 to react with the reagent in the septal layer 4, the reagent is sucked into the test paper after a user collects blood, the current on the loops of the contrast electrode 31 and the working electrode 32 is transmitted to a tester for detection through the output end of the circuit loop, the tester converts the collected current into concentration and displays the measurement result through a display screen, and the detection accuracy of the test paper on the test paper can be improved through the arrangement of the double detection lines of the contrast electrode 31, the working electrode 32 and the full blood electrode 33.
Example 2
The embodiment of the application discloses uric acid detection test paper. Referring to fig. 1 and 3, the present embodiment is different from embodiment 1 in that: the position and shape of the vent holes are different. Specifically, in the present embodiment, the second exhaust hole 41 having an opening facing the side is opened at one end of the partition layer 4 close to the sample inlet 61, the second exhaust hole 41 is substantially rectangular, and the second exhaust hole 41 is perpendicular to the opening direction of the sample inlet 61.
The implementation principle of the embodiment 2 is as follows: the test sample flows into the test paper through the test sample inflow port 61, and sequentially passes through a loop formed by the silver lead 2 and the carbon layer electrode 3 on the substrate 1 to react with the reagent in the interlayer 4, the test sample after blood collection by a user sucks the reagent, the current on the loops of the contrast electrode 31 and the working electrode 32 is transmitted to a tester for detection through the output end of the circuit loop, and the second exhaust hole 41 is arranged in the interlayer 4, so that the gas generated when the test sample flows into the test paper can be reduced, the purity of the test sample is improved, and the accuracy of test of the test sample is improved.
Example 3
The embodiment of the application discloses uric acid detection test paper. Referring to fig. 1 and 4, the present embodiment is different from embodiment 1 in that: the position and shape of the vent holes are different. Specifically, in the present embodiment, the end of the middle partition layer 4 close to the sample inflow port 61 is provided with a third exhaust hole 42 with an opening facing to the side, the third exhaust hole 42 is substantially rectangular, and the third exhaust hole 42 is perpendicular to the opening direction of the sample inflow port 61; the second exhaust hole 41 and the third exhaust hole 42 have the same size and are opened in opposite directions.
The implementation principle of the embodiment 3 is as follows: the test sample flows into the test paper through the test sample inflow port 61, and sequentially passes through a loop formed by the silver lead 2 and the carbon layer electrode 3 on the substrate 1 to react with the reagent in the interlayer 4, the test sample after blood collection by a user sucks the reagent, the current on the loops of the contrast electrode 31 and the working electrode 32 is transmitted to a tester for detection through the output end of the circuit loop, and the second exhaust hole 41 is arranged in the interlayer 4, so that the gas generated when the test sample flows into the test paper can be reduced, the purity of the test sample is improved, and the accuracy of test of the test sample is improved.
The above embodiments are preferred embodiments of the present application, and the protection scope of the present application is not limited by the above embodiments, so: equivalent changes in structure, shape and principle of the present application shall be covered by the protection scope of the present application.
Claims (8)
1. The uric acid test paper is characterized in that: including substrate (1), carbon layer electrode (3), well interlayer (4), adhesion layer (6) and upper cover layer (7), the one end of substrate (1) is equipped with the body and flows into the end, the other end of substrate (1) is equipped with circuit loop output, be equipped with wire (2) on substrate (1), be equipped with on well interlayer (4) and be used for detecting detection reagent (5) of uric acid, carbon layer electrode (3) are including full blood electrode (33), working electrode (32) and contrast electrode (31).
2. The uric acid test strip according to claim 1, characterized in that: the detection reagent (5) is arranged on one side of the middle interlayer (4) close to the inflow end of the sample.
3. The uric acid test strip according to claim 1, characterized in that: the lead (2) is a silver lead (2), and three electrode contact points of the silver lead (2) and the carbon layer electrode (3) are in short circuit or open circuit to serve as detection marks.
4. The uric acid test strip according to claim 1, characterized in that: the contrast electrode (31) and working electrode (32) circuit provides a sample trigger function, and the contrast electrode (31) and full blood electrode (33) circuit provides a full blood identification function.
5. The uric acid test strip according to claim 1, characterized in that: the middle interlayer (4) is provided with a second exhaust hole (41) or a third exhaust hole (42).
6. The uric acid test strip according to claim 1, characterized in that: the upper cover layer (7) is provided with a first exhaust hole (71).
7. The uric acid test strip according to claim 1, characterized in that: the carbon layer electrode (3), the middle interlayer (4), the adhesion layer (6) and the upper cover layer (7) are sequentially arranged on the substrate (1) in an overlapping mode.
8. The uric acid test strip according to claim 3, characterized in that: the carbon layer electrode (3) and the silver wire (2) are provided with identification pins with corresponding quantity and positions, so that the carbon layer and the silver wire (2) are simultaneously short-circuited or open-circuited.
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