CN216979100U

CN216979100U - Sample analysis apparatus

Info

Publication number: CN216979100U
Application number: CN202123204181.4U
Authority: CN
Inventors: 陈宇; 张少维; 李江波; 汪云; 程蛟; 李成杰
Original assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd
Current assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd
Priority date: 2021-12-17
Filing date: 2021-12-17
Publication date: 2022-07-15
Anticipated expiration: 2031-12-17

Abstract

The utility model discloses sample analysis equipment, which comprises a sample mechanism, a sample storage mechanism and a sample analysis module, wherein the sample mechanism is provided with at least one sample position; the reaction mechanism is provided with at least one reaction site; the sample dispensing mechanism comprises a first moving component and a sample needle arranged on the first moving component, the sample needle comprises a first main body part and a first needle tip part, the first needle tip part is connected with the first main body part, the outer diameter of the first main body part is larger than that of the first needle tip part, the outer diameter of the first needle tip part is constant, and the outer diameter is 0.45 mm-0.6 mm; a measuring mechanism for measuring a reaction solution to be measured; the ultrasonic cleaning mechanism comprises a cleaning pool and an ultrasonic sound source assembly, wherein the cleaning pool is used for containing cleaning liquid, and the ultrasonic sound source assembly is used for providing sound field vibration for the cleaning liquid in the cleaning pool; and a liquid path support mechanism for supplying the cleaning liquid into the cleaning tank. The sample analysis equipment can not only realize the cleanness of the sample needle before sampling, but also avoid the bending hidden trouble caused by the thinning of the tip part of the first needle.

Description

Sample analysis apparatus

Technical Field

The utility model relates to the technical field of medical instruments, in particular to sample analysis equipment.

Background

The sample analysis device comprises a sample needle which is used for collecting a sample in a sample container and can discharge the sample into a reaction container so that the sample is mixed with a reagent to form a reaction liquid. In the use process, the sample needle needs to be frequently contacted with different samples, and the samples remained on the outer surface of the sample needle are easy to cause cross contamination, so that the detection result is influenced.

SUMMERY OF THE UTILITY MODEL

The present invention is directed to solving at least one of the problems of the prior art. Therefore, the utility model provides a sample analysis device which can reduce the area of the outer peripheral surface of the needle tip part, thereby reducing the residue of liquid.

A sample analysis apparatus according to a first embodiment of the present invention includes:

the sample mechanism is provided with at least one sample position, the sample position is used for placing a sample container, and the sample container is used for accommodating a sample;

the reaction mechanism is provided with at least one reaction position, the reaction position is used for placing a reaction container and incubating reaction liquid in the reaction container, and the reaction liquid is prepared by the sample;

a sample dispensing mechanism including a first moving member and a sample needle provided on the first moving member, the first moving member for driving the sample needle to move between the sample site and the reaction site to aspirate a sample at the sample site and discharge the sample at the reaction site through the sample needle, the sample needle including a first body portion and a first needle tip portion connected to the first body portion, the first body portion having an outer diameter larger than that of the first needle tip portion, wherein the first needle tip portion has a constant outer diameter and an outer diameter of 0.45mm to 0.6 mm;

a measuring unit for measuring the reaction solution incubated in the reaction container;

an ultrasonic cleaning mechanism including a cleaning tank for containing a cleaning liquid into which at least a portion of the first needle tip portion of the sample needle is inserted to clean the sample needle, and an ultrasonic sound source assembly for supplying sound field vibration to the cleaning liquid in the cleaning tank;

a liquid path support mechanism for supplying the cleaning liquid into the cleaning tank.

The sample analysis device provided by the embodiment of the utility model has at least the following beneficial effects:

sample analysis equipment has reduced the outer peripheral face area of first needle point portion through the mode of reducing first needle point portion external diameter to can reduce the area of adhering to of liquid, promptly, when first needle point portion breaks away from the sample liquid level, the sample of adhering to on it will reduce, be convenient for carry out subsequent washing step. Meanwhile, the sample analysis equipment carries out non-contact type cleaning through the ultrasonic cleaning mechanism, interference of human factors can be eliminated, cleaning of the sample needle before sampling can be achieved, and bending hidden danger caused by thinning of the tip of the first needle can be avoided.

In other embodiments of the present invention, the first needle tip has an inner diameter of 0.25mm to 0.4 mm.

In other embodiments of the present invention, the first body portion has an inner diameter of 0.6mm to 0.8 mm.

In other embodiments of the present invention, the sample needle further comprises a first transition portion connected between the first needle tip portion and the first body portion;

wherein the outer diameter of the first transition portion tapers in a direction from the first body portion to the end of the first needle tip portion, wherein the taper angle of the outer wall of the first transition portion is less than or equal to 70 °;

and/or the inner diameter of the first main body part is larger than that of the first needle tip part, and the inner diameter of the first transition part is gradually reduced along the direction from the first main body part to the end part of the first needle tip part, wherein the taper angle of the inner wall of the first transition part is smaller than or equal to 70 degrees.

In other embodiments of the present invention, the sample analysis device further comprises:

the reagent mechanism is provided with at least one reagent position, the reagent position is used for placing a reagent container, and the reaction liquid is prepared by the sample and the reagent;

reagent divides injection mechanism, reagent divides injection mechanism includes second removal part and reagent needle, the second removal part is used for the drive the reagent needle is in the reagent position and move between the reaction position, in order to pass through the reagent needle is in the reagent position absorbs reagent and in the reaction position discharges reagent, the reagent needle includes second main part and second needle point portion, second needle point portion connect in second main part and external diameter are invariable, the external diameter of second main part is greater than the external diameter of second needle point portion, the external diameter of second needle point portion is 0.7mm to 1.0 mm.

In other embodiments of the present invention, the second needle tip portion has an inner diameter of 0.5mm to 0.8 mm.

In other embodiments of the present invention, the second needle tip portion has a length to inner diameter ratio of less than or equal to 10.

In other embodiments of the present invention, the second body portion has an inner diameter of 1.4mm to 1.8 mm.

In other embodiments of the present invention, the reagent needle further comprises a second transition portion connected between the second tip portion and the second body portion;

wherein, along the direction from the second main body part to the end part of the second needle tip part, the outer diameter of the second transition part is gradually reduced, and the taper angle of the outer wall of the second transition part is less than or equal to 12 degrees;

and/or the inner diameter of the second main body part is larger than that of the second needle tip part, the inner diameter of the second transition part is gradually reduced along the direction from the second main body part to the end part of the second needle tip part, and the taper angle of the inner wall of the second transition part is smaller than or equal to 12 degrees.

the reagent dispensing mechanism comprises a second moving component and a reagent needle, wherein the second moving component is used for driving the reagent needle to move between the reagent position and the reaction position so as to suck a reagent at the reagent position and discharge the reagent at the reaction position through the reagent needle, the reagent needle comprises a third main body part and a third needle tip part, the third needle tip part is connected with the third main body part, the outer diameter of the third main body part is larger than that of the third needle tip part, the outer diameter of the third needle tip part is gradually reduced along the direction from the third main body part to the end part of the third needle tip part, the outer diameter of the end part of the third needle tip part is 0.7mm to 0.9mm, and the taper angle of the outer wall of the third needle tip part is smaller than or equal to 6 degrees.

In other embodiments of the present invention, the third body portion has an inner diameter of 1.4mm to 1.8 mm.

In other embodiments of the present invention, the ultrasonic sound source assembly is located at the bottom of the cleaning tank to transmit sound field vibration to the cleaning liquid in the cleaning tank through the bottom wall of the cleaning tank.

In other embodiments of the present invention, the cleaning pool is provided with a cleaning chamber and an overflow chamber, the sample needle is inserted into the cleaning liquid in the cleaning chamber, and the overflow chamber is used for receiving the cleaning liquid overflowing from the cleaning chamber.

A sample analysis apparatus according to a second embodiment of the present invention includes:

a sample dispensing mechanism including a first moving member and a sample needle provided on the first moving member, the first moving member being configured to drive the sample needle to move between the sample position and the reaction position to draw a sample at the sample position and discharge the sample at the reaction position through the sample needle, the sample needle including a fourth body portion and a fourth tip portion, the fourth tip portion being connected to the fourth body portion, an outer diameter of the fourth body portion being larger than an outer diameter of the fourth tip portion, the outer diameter of the fourth tip portion being gradually reduced in a direction from the fourth body portion to an end of the fourth tip portion, wherein the outer diameter of the end of the fourth tip portion is 0.45mm to 0.6mm, and a taper angle of an outer wall is less than or equal to 6 °;

a measuring mechanism for measuring a reaction solution to be measured;

an ultrasonic cleaning mechanism including a cleaning tank for containing a cleaning liquid into which at least a portion of the fourth needle-tip portion of the sample needle is inserted to clean the sample needle, and an ultrasonic sound source assembly for supplying sound field vibration to the cleaning liquid in the cleaning tank;

In other embodiments of the present invention, an inner diameter of an end of the fourth needle tip portion is 0.29mm to 0.33 mm.

A sample analysis apparatus according to a third embodiment of the present invention includes:

a sample dispensing mechanism including a first moving member and a sample needle provided on the first moving member, the first moving member for driving the sample needle to move between the sample position and the reaction position to aspirate a sample at the sample position and discharge the sample at the reaction position through the sample needle, the sample needle including a fifth body portion and a fifth needle tip portion, the fifth needle tip portion being connected to the fifth body portion and having a constant outer diameter, the fifth body portion having an outer diameter larger than that of the fifth needle tip portion, wherein the fifth needle tip portion has an outer diameter of 0.45mm to 0.6mm, and at least an outer surface of the fifth needle tip portion is provided with a polymer coating having a contact angle larger than 90 °;

and a measuring mechanism for measuring the reaction solution to be measured.

A sample analysis apparatus according to a fourth embodiment of the present invention includes:

a sample dispensing mechanism including a first moving member and a sample needle provided on the first moving member, the first moving member is used for driving the sample needle to move between the sample position and the reaction position, to aspirate a sample at the sample site and discharge the sample at the reaction site through the sample needle, the sample needle comprising a sixth body portion and a sixth needle tip portion, the sixth needle tip portion is connected to the sixth body portion, the outer diameter of the sixth body portion is larger than the outer diameter of the sixth needle tip portion, the outer diameter of the sixth needle tip portion is gradually reduced in a direction from the sixth body portion to an end portion of the sixth needle tip portion, wherein the outer diameter of the end part of the sixth needle tip part is 0.45mm to 0.6mm, the taper angle of the outer wall is less than or equal to 6 degrees, and at least the outer surface of the sixth needle tip portion is provided with a polymer coating, and the contact angle of the polymer coating is larger than 90 degrees;

and a measuring mechanism for measuring the reaction solution to be measured.

In other embodiments of the present invention, the contact angle of the polymer coating is greater than or equal to 120 °.

Additional aspects and advantages of the utility model will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the utility model.

Drawings

The utility model is further described with reference to the following figures and examples, in which:

FIG. 1 is a schematic plan view of a sample analysis apparatus in an embodiment of the present invention;

FIG. 2 is a schematic cross-sectional view of a sample needle in accordance with an embodiment of the present invention;

FIG. 3 is a schematic top perspective view of an ultrasonic cleaning mechanism for a sample needle in accordance with one embodiment of the present invention;

FIG. 4 is a schematic side view of the ultrasonic cleaning mechanism for the sample needle of FIG. 3;

FIG. 5 is a schematic perspective view of an ultrasonic cleaning mechanism for a sample needle in accordance with another embodiment of the present invention;

FIG. 6 is a side perspective view of the ultrasonic cleaning mechanism for the sample needle of FIG. 5;

FIG. 7 is a schematic sectional view of a reagent needle according to an embodiment of the present invention;

FIG. 8 is a schematic sectional view of a reagent needle according to another embodiment of the present invention;

FIG. 9 is a schematic cross-sectional view of a sample needle in another embodiment of the present invention.

Reference numerals:

a sample mechanism 10;

a sample dispensing mechanism 20, a sample needle 21, a first body portion 211, a first tip portion 212, a first transition portion 213, a fourth body portion 214, a fourth tip portion 215, and a first moving member 22;

a sample needle normal washing mechanism 30;

a sample needle ultrasonic cleaning mechanism 40, a cleaning cavity 41, an ultrasonic sound source assembly 42, an overflow cavity 43, a liquid inlet 44, a first waste liquid outlet 45 and a second waste liquid outlet 46;

a reagent mechanism 50;

a reagent dispensing mechanism 60, a reagent needle 61, a second body portion 611, a second tip portion 612, a second transition portion 613, a third body portion 614, a third tip portion 615, and a second moving member 62;

a reagent needle normal washing mechanism 70;

a reaction mechanism 80;

a blending mechanism 90;

a measurement unit 100;

the reagent needle ultrasonic cleaning mechanism 110.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.

In the description of the present invention, it should be understood that the orientation or positional relationship referred to in the description of the orientation, such as the upper, lower, front, rear, left, right, etc., is the orientation or positional relationship described based on the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.

In the description of the present invention, the meaning of a plurality is one or more, the meaning of a plurality is two or more, and the above, below, exceeding, etc. are understood as excluding the present numbers, and the above, below, within, etc. are understood as including the present numbers. If the first and second are described for the purpose of distinguishing technical features, they are not to be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.

In the description of the present invention, unless otherwise explicitly limited, terms such as arrangement, installation, connection and the like should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above terms in the present invention in combination with the specific contents of the technical solutions.

In the description of the present invention, reference to the description of the terms "one embodiment," "some embodiments," "an illustrative embodiment," "an example," "a specific example," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

Referring to fig. 1, a schematic plan view of a sample analysis apparatus according to an embodiment of the present invention is shown, in which an arc-shaped broken line indicates a rotation locus of a rotating member. The sample analysis apparatus according to the embodiment of the present invention includes a sample mechanism 10, a sample dispensing mechanism 20, a sample needle cleaning mechanism, a reaction mechanism 80, a kneading mechanism 90, and a measurement mechanism 100, and a fluid path support mechanism cooperating with the sample needle cleaning mechanism is further provided in the present embodiment, which will be described in detail below with reference to the drawings.

The sample mechanism 10 is used for carrying a sample to be injected. For example, the Sample mechanism 10 may be implemented by a Sample Delivery Module (SDM) and a front end track. In other embodiments, it may also be a rotary sample tray, and the sample tray is provided with at least one sample position for placing a sample container such as a sample tube, and the sample can be dispatched to a corresponding position, such as a position for the sample dispensing mechanism 20 to suck the sample, by the rotation of the sample tray.

The sample dispensing mechanism 20 includes a sample needle 21 and a first moving member 22. The first moving member 22 can drive the sample needle 21 to move to suck the sample and discharge it into the reaction container located at the reaction site. In some embodiments, the data of the sample needle 21 may be one or more pieces. In some embodiments, when the sample needles 21 are plural, the first moving member 22 may drive the sample needles 21 to move independently of each other. The first moving part 22 may be a two-dimensional or three-dimensional driving mechanism, which may be designed according to specific requirements. For the sample needle 21, a typical set of timing actions is such that: the sample needle 21 moves to, for example, a sample site to aspirate a sample, then moves to, for example, a reaction site to discharge the aspirated sample to a reaction vessel, and then moves to a predetermined position to be washed; of course, in some examples, the sample needle 21 may be cleaned once before the sample needle 21 sucks the sample and discharges the sample.

The sample needle cleaning mechanism is used for cleaning the sample needle 21 so as to prevent the residual sample on the sample needle 21 from contacting with other samples and generating cross contamination.

The reaction mechanism 80 is used for carrying a reaction container and is provided with at least one reaction site, the reaction site is used for placing the reaction container so as to hatch reaction liquid in the reaction container, and the reaction liquid is prepared by at least a sample and a reagent together. In some embodiments, the reaction mechanism 80 may be a rotary reaction tray, and the reaction tray is provided with a plurality of reaction sites, and specifically, the reaction tray can rotate and drive the reaction containers in the reaction sites thereof to rotate, so as to schedule the reaction containers in the reaction tray and incubate the reaction solution in the reaction containers.

The mixing mechanism 90 is used for mixing the reaction solution to be mixed uniformly in the reaction vessel. For example, the kneading mechanism 90 kneads the reaction solution at the kneading position.

The measurement means 100 may be a photometric device for performing photometric measurement on the reaction solution after completion of incubation to obtain reaction data of the sample. For example, the optical measuring unit may detect the absorbance of a reaction solution to be measured, and calculate the concentration of a component to be measured in a sample from a calibration curve.

Based on the above, the sample needle 21 is an important component for sample transfer, and the tip portion thereof needs to be frequently contacted with a sample, so as to avoid cross contamination caused by the sample remained in the previous sampling step contacting with the subsequent sample through the sample needle, and to ensure the cleanliness of the sample needle 21 before each sampling. The present embodiment proposes a sample needle 21 that reduces the outer surface area of the needle tip by reducing the outer diameter of the needle tip, thereby reducing the residue of liquid on the outer surface of the needle tip.

The sample needle 21 is generally an elongated tubular structure, and is made of a material (for example, a metal) which has a certain strength and is not easy to be subjected to liquid hanging, specifically referring to fig. 2, fig. 2 is a schematic cross-sectional view of the sample needle in this embodiment, and is divided according to functions, the sample needle 21 may include a first main body portion 211 and a first needle tip portion 212, an outer diameter of the first main body portion 211 is larger than an outer diameter of the first needle tip portion 212, under driving of a driving mechanism such as a syringe, sample liquid can be sucked into the sample needle 21 through an opening at a front end of the first needle tip portion 212, and can also be discharged out of the sample needle 21 through the opening, the sample liquid is sucked and stored in the first needle tip portion 212, the first main body portion 211 is connected to a rear end of the first needle tip portion 212, the first main body portion 211 is generally long, and is for clearly showing a structure of the sample needle, and fig. 2 only shows a partial structure of the first main body portion 211.

As shown in the figure, the first needle tip portion 212 in the present embodiment has a cylindrical structure, and the outer diameter is kept constant, since the first needle tip portion 212 is a sample needle 21 contacting the sampleThereby reducing fluid residue on the first needle tip 212 and helping to avoid cross-contamination. To achieve the objective of reducing residue, the present embodiment reduces the outer diameter dimension of the first needle tip portion 212, specifically, the outer diameter D of the first needle tip portion 212₁0.45mm to 0.6mm, according to the calculation formula of the area of the outer peripheral surface of the cylinder, when the outer diameter is reduced, the area of the outer peripheral surface of the first needle tip portion 212 is reduced simultaneously, so that the attachment area of the liquid can be reduced, that is, when the first needle tip portion 212 is separated from the liquid surface of the sample, the residual sample attached to the first needle tip portion is reduced, thereby facilitating the subsequent cleaning step; meanwhile, after the area of the outer peripheral surface of the first needle tip portion 212 is reduced, larger droplets do not adhere to the outer surface of the first needle tip portion 212.

Specifically, two kinds of sample needles were selected, each having a cylindrical tip portion, and the length L of the tip portion extending into the sample liquid during sampling was set to 5mm, the sample needle a had an outer diameter size according to the related art, and the sample needles b to d had an outer diameter size according to the present embodiment, and the outer peripheral surface area S calculated from the above data was shown in table 1, and the outer peripheral surface area S was decreased in synchronization with the decrease in the outer diameter.

TABLE 1

It should be noted that the first needle tip portion 212 may have any value within the range of 0.45mm to 0.6mm, and is not limited to the values listed in the above table.

Typically, the sample needle cleaning mechanism includes a sample needle normal cleaning mechanism for performing normal mode cleaning of the sample needle. A common cleaning mechanism for a sample needle is used for cleaning operations such as washing and/or soaking the sample needle with a cleaning liquid and/or a cleaning agent or the like. The cleaning solution herein refers to a liquid medium such as physiological saline, distilled water or diluent, and the cleaning agent refers to some medium that can chemically react with the pollutant on the object to be cleaned, for example, some acidic liquid, etc. The common cleaning mechanism for the sample needle can be realized by adopting some existing or future cleaning structures.

However, it is difficult to completely remove the contamination on the outer wall surface of the sample needle 21 by merely washing and/or soaking with the cleaning solution and/or the cleaning agent, and therefore, the cleaning is usually performed manually, in this case, along with the reduction of the outer diameter of the first needle tip portion 212, the strength of the first needle tip portion 212 is also reduced correspondingly, which causes the first needle tip portion 212 to be easily bent during the cleaning process, and in severe cases, even causes the sample needle 21 to be broken, so that the utility model in the field usually does not consider reducing the liquid residue in a manner of reducing the outer diameter of the first needle tip portion 212. In view of the above problem, the present embodiment employs a non-contact cleaning method to remove the liquid remained on the first needle tip portion 212, thereby avoiding the damage of the first needle tip portion 212. The term "non-contact washing" as used herein means that the operator does not directly contact the sample needle, or the operator indirectly contacts the sample needle 21 via a tool, thereby eliminating the damage to the sample needle due to human errors.

Referring to fig. 1, the sample needle cleaning mechanism includes a sample needle ultrasonic cleaning mechanism 40 for performing non-contact cleaning, which is used for ultrasonic mode cleaning of the sample needle. Ultrasonic mode cleaning or ultrasonic cleaning (ultrasonic cleaning) is to disperse, emulsify and strip the pollutants by utilizing the direct and indirect actions of cavitation action, acceleration action and/or direct current action of ultrasonic waves in liquid on the liquid and the pollutants to achieve the aim of cleaning. Generally, ultrasonic cleaning can peel off and remove dirt on the surface of an object to be cleaned by strong cavitation and vibration generated by ultrasonic waves, and can decompose and emulsify greasy dirt. In some embodiments, the media used for ultrasonic mode cleaning herein comprises a chemical agent, such as the cleaning agents described above. The chemical agent may be an alkaline cleaning solution, such as sodium hydroxide (NaOH) or potassium hydroxide (KOH). In addition, surface active components may be added to the chemical agents to enhance the cleaning effect. In some embodiments, after the cleaning solution is used to perform one-time ultrasonic cleaning on the object to be cleaned, the deionized water is used to perform ultrasonic cleaning on the object to be cleaned again to scrub the object once, so that the cleaning solution is prevented from remaining on the object to be cleaned to influence the test result of the subsequent test. Since the ultrasonic cleaning has an excellent cleaning effect, the sample needle 21 does not need to be manually wiped to remove stains on the surface of the sample needle 21, and the sample needle 21 is prevented from being deformed, so that the sample needle 21 can be thinned to reduce sample residues.

It can be understood that, referring to fig. 1, the sample needle cleaning mechanism in some embodiments may include both the sample needle ultrasonic cleaning mechanism 40 and the sample needle normal cleaning mechanism 30, and both the sample needle normal cleaning mechanism 30 and the sample needle ultrasonic cleaning mechanism 40 can be used for cleaning the sample needle 21. In some embodiments, the sample needle normal washing mechanism 30 is disposed on the movement trajectory of the sample needle 21, so that the sample needle 21 can be moved to the sample needle normal washing mechanism 30 for washing. Similarly, in some embodiments, the sample needle ultrasonic cleaning mechanism 40 is disposed on the motion trajectory of the sample needle 21, such that the sample needle 21 can be moved to the sample needle ultrasonic cleaning mechanism 40 to be cleaned.

Referring to fig. 3 to 6, the sample needle ultrasonic cleaning mechanism 40 is for effecting non-contact cleaning of the sample needle 21, and the sample needle ultrasonic cleaning mechanism 40 includes a cleaning tank for containing a cleaning liquid, and an ultrasonic sound source assembly 42, at least a portion of the first needle tip portion 212 of the sample needle 21 being inserted into the cleaning liquid in the cleaning tank, thereby enabling the sample needle 21 to be cleaned. The ultrasonic sound source assembly 42 is used to provide sound field vibration to the cleaning fluid in the ultrasonic cleaning tank and the fluid path support mechanism is used to provide cleaning fluid into the cleaning tank. In the cleaning process, the first needle tip portion 212 only contacts with the cleaning liquid, and does not contact with an operator or other tools, so that the first needle tip portion 212 can be effectively prevented from bending due to the fact that the first needle tip portion contacts with a foreign object.

Through the above, the analytical equipment of this embodiment can realize steps such as sample, detection, washing through the cooperation of each mechanism and each part, especially to the washing link, and sample needle 21 can independently move to the washing pond in and carry out non-contact washing, gets rid of the interference of human factor, combines above-mentioned scheme that reduces pinpoint portion outer peripheral face area in order to reduce the sample and remain, and this embodiment can enough realize the abundant cleanness of sample needle 21 before the sample, can avoid again because of the bending hidden danger that pinpoint portion becomes thin and lead to.

In some embodiments of the present invention, the ultrasonic sound source assembly 42 is located at the bottom of the cleaning tank, specifically, the cleaning tank includes a cleaning chamber 41, the cleaning chamber 41 is used for carrying a cleaning liquid, so that the sample needle 21 can at least partially extend into the cleaning chamber 41, the ultrasonic sound source assembly 42 is specifically located at the bottom of the cleaning chamber 41, the vibration of the sound field is transmitted to the cleaning liquid through the bottom wall of the cleaning chamber 41, the wall of the cleaning chamber 41 vibrates during the cleaning process, and the dirt substance cleaned does not adhere to the wall of the cleaning chamber 41, so that the cleaning of the cleaning chamber 41 is maintained, and it can be understood that, due to the gravity, the dirt is more easily attached to the bottom of the cleaning chamber 41, but since the ultrasonic sound source assembly 42 is disposed at the bottom of the cleaning chamber 41 in the present embodiment, the bottom wall of the cleaning chamber 41 vibrates more, and the dirt can be effectively prevented from being attached to the bottom wall of the cleaning chamber 41. In addition, the ultrasonic sound source assembly 42 is arranged at the bottom of the cleaning pool, and the center of gravity of the ultrasonic sound source assembly 42 and the center shaft of the cleaning pool are on the same line by adopting a bottom seismic source mode, so that the center of gravity can not shift even after long-time use.

In order to realize liquid inlet and liquid discharge of the cleaning liquid, the cleaning pool further comprises a liquid inlet 44 and a first waste liquid outlet 45, the liquid inlet 44 is located on the lateral side of the cleaning pool and communicated with the cleaning cavity 41, and the first waste liquid outlet 45 is arranged at the bottom of the cleaning pool and also communicated with the cleaning cavity 41. It is understood that the sample needle ultrasonic cleaning mechanism 40 may discharge the cleaning liquid in the cleaning chamber 41 through the first waste liquid discharge port 45 after the ultrasonic cleaning is completed; the cleaning liquid in the cleaning chamber 41 may be discharged during the ultrasonic cleaning process, and may be set according to the cleaning requirement. By providing the first waste liquid discharge port 45, the cleaning liquid after the ultrasonic cleaning in the cleaning chamber 41 can be completely or as much as possible discharged, and the dirt is prevented from remaining in the cleaning chamber 41, thereby preventing the remaining dirt from affecting the next ultrasonic cleaning.

In some embodiments, referring to fig. 3 and 4, the cleaning pool further includes an overflow chamber 43, and a second waste liquid outlet 46 communicated with the overflow chamber 43, the overflow chamber 43 is used for receiving the cleaning liquid overflowed from the cleaning chamber 41, and the second waste liquid outlet 46 is used for discharging the liquid overflowed from the cleaning chamber 41, so that the amount of the cleaning liquid in the cleaning chamber 41 can be kept constant, and the consistency of energy in the cleaning process can be improved.

Referring to fig. 3, in some embodiments, an overflow chamber 43 is disposed around the wash chamber 41, wherein the height of the side wall of the wash chamber 41 is less than the height of the side wall of the overflow chamber 43. It can be understood that, in general, the sample needle ultrasonic cleaning mechanism is placed horizontally, the upper surface of the cleaning cavity 41 is located on the same horizontal plane, and when the liquid volume of the cleaning cavity 41 being injected is larger than the volume of the cleaning cavity 41, the cleaning liquid can overflow from any direction, so that the overflow cavity 43 is arranged around the cleaning cavity 41, and can receive the liquid overflowing from any direction of the cleaning cavity 41, thereby having a better overflow liquid receiving effect.

In some embodiments of the present invention, referring to fig. 2, the inner diameter d of the first needle tip 212₁Is 0.25mm to 0.4mm, one end of the first main body part 211 departing from the first needle tip part 212 is connected with a sample needle liquid path component, the sample needle liquid path component comprises a water tank, a pumping mechanism, a control valve and an injector which are sequentially connected through a connecting pipeline, one end of the injector is connected with the control valve through the connecting pipeline, the other end of the injector is connected with the first main body part 211 through the connecting pipeline, when the piston in the injector reciprocates, water in the pipeline can enter the piston cavity of the injector, or flows out of the piston cavity, so that negative pressure or positive pressure is formed in the pipeline, and the sample needle is driven to suck or discharge the sample, in order to avoid the water in the liquid path component from being polluted by the sample, an air section exists between the sample and the water in the connecting pipeline when the sample is sucked, that is, a section of air is sucked before the sample is sucked, and it is necessary to avoid the bubble in the air section from breaking during the sucking and discharging of the sample. When water flows in the pipeline, the expansion and contraction of the soft pipeline can be caused, so that the inner diameter of the pipeline is changed, and the accuracy of the sample suction of the sample needle 21 is influencedFlow resistance when flowing in the first needle tip portion 212, so, when the flow velocity and the flow of the liquid medium in the pipe change because of the elastic deformation of the pipe, because the relatively large flow resistance needs to be overcome in the flow of the sample liquid in the first needle tip portion 212, the amplitude that it is influenced will correspondingly reduce, thereby the accuracy of sampling by the sample needle 21 is guaranteed to a certain extent.

In some embodiments of the present invention, the first body portion 211 is also a cylindrical structure with a constant inner diameter and a size ranging from 0.6mm to 0.8mm, and as mentioned above, the syringe transmits pressure through the liquid medium to achieve the aspiration and the drainage of the sample needle, so that the liquid medium also enters the first body portion 211. Along with the increase of the volume of the sample liquid in the first main body part 211, the volume of the corresponding liquid medium can be reduced simultaneously, and in the process of retreating the liquid medium, because the inner wall of the first main body part 211 is difficult to realize complete smoothness, a small amount of the liquid medium can remain on the inner wall, and the remaining liquid medium can be mixed with the sample liquid to dilute the sample liquid, so that the measurement result is affected. Based on this, the present embodiment employs the first body portion 211 having a larger inner diameter, which is dependent on the diameter of the cylinder (the inner diameter d mentioned above) according to the calculation formula of the area and volume of the outer peripheral surface of the cylinder₂) That is, although the area of the outer circumferential surface of the cylinder (the area of the inner wall surface of the first body portion 211) increases synchronously with the increase in the diameter, which leads to a synchronous increase in the residual amount of the liquid medium, the volume (the volume of the first body portion 211) increases more, and the sample liquid is contained more, so that the dilution rate of the sample liquid can be reduced.

In some embodiments of the present invention, the sample needle 21 further includes a first transition portion 213, and the first transition portion 213 can realize transition connection between the first body portion 211 and the first needle tip portion 212, so as to prevent abrupt change of the tube diameter. The internal diameter of first main part 211 is greater than the internal diameter of first pinpoint portion 212, and follow the direction of first main part 211 to first pinpoint portion 212, the internal diameter of first transition portion 213 reduces gradually, the less head end of its internal diameter is connected in the tail end of first pinpoint portion 212, the great tail end of internal diameter is connected in the head end of first main part 211, the cone angle alpha of first transition portion 213 inner wall is less than or equal to 70, can avoid sample needle 21's internal diameter to take place the sudden change through setting up first transition portion 213, guarantee the steady flow of sample liquid, also can avoid simultaneously that the velocity of flow changes too big and lead to the bubble in the air section to break, avoid the sample to pollute liquid in the sample needle liquid path subassembly. In some embodiments, the outer diameter of the first main body portion 211 is larger than the outer diameter of the first needle tip portion 212, and the outer diameter of the first transition portion 213 is also gradually reduced along the direction from the first main body portion 211 to the first needle tip portion 212, that is, the first transition portion 213 may have a conical structure in the figure, so that the risk of breaking the sample needle 21 due to sudden change of the outer diameter can be avoided. The taper angle of the outer wall and the inner wall of the first transition portion 213 may be the same, i.e., the wall thickness of the first transition portion 213 is uniform. It should be noted that, in the present embodiment, a relatively large outer wall taper angle range is adopted, so that the length of the first transition portion 213 can be shortened, which is beneficial for the mold to process the first transition portion 213 by roll forming.

Referring to fig. 1, the analysis apparatus in the embodiment of the present invention may further include a reagent mechanism 50 and a reagent dispensing mechanism 60, where the reagent mechanism 50 is configured to carry a reagent. In an embodiment, the reagent mechanism 50 is disposed in a disc-shaped structure, the reagent mechanism 50 has a plurality of reagent sites for carrying reagent containers, and the reagent mechanism 50 can rotate and drive the reagent containers carried by the reagent mechanism 50 to rotate, so as to rotate the reagent containers to the reagent sites, so that the reagent dispensing mechanism 60 can aspirate the reagent. In one embodiment, the reagent mechanism 50 is a single one, which may be separately disposed outside the reaction mechanism 80.

The reagent dispensing mechanism 60 includes a reagent needle 61 and a second moving member 62. The second moving member 62 can drive the reagent needle 61 to move to aspirate and discharge the reagent into the reaction vessel located at the reagent addition site. In some embodiments, the reagent needle 61 may be one or more. In some embodiments, when the reagent needle 61 is plural, the second moving member 62 may drive the reagent needles 61 to move independently of each other. The second moving member 62 may be a two-dimensional or three-dimensional driving mechanism, which may be designed according to specific requirements. For the reagent needle 61, a typical set of time-sequential actions is such that: the reagent needle 61 is moved to, for example, a reagent site to aspirate a reagent, then moved to, for example, a reagent addition site to discharge the aspirated reagent into a reaction vessel, and then moved to a predetermined position to be washed; of course, in some examples, the outer wall of the reagent needle 61 may be cleaned once after the reagent needle 61 sucks the reagent and before the reagent is discharged. In some embodiments, when the reagent needle is set to consecutively aspirate a plurality of reagents and then discharge them together, the reagent needle 61 consecutively performs a plurality of aspiration reagent operations to aspirate a desired plurality of reagents; wherein the outer wall of the reagent needle 61 can be cleaned after completing one reagent sucking operation and before starting the next reagent sucking operation in the process of sucking a plurality of required reagents.

Referring to fig. 7, fig. 7 is a cross-sectional view of the reagent needle 61 in the present embodiment, similar to the sample needle 21, the reagent needle 61 is also an elongated tubular structure and is divided according to functions, the reagent needle 61 includes a second body portion 611 and a second needle tip portion 612, the outer diameter of the second body portion 611 is larger than the outer diameter of the second needle tip portion 612, under the driving of the driving mechanism, the reagent can be sucked into the reagent needle 61 through an opening at the head end of the second needle tip portion 612 and can also be discharged out of the reagent needle 61 through the opening, the second body portion 611 is connected to the tail end of the second needle tip portion 612, the second body portion 611 is generally long, and in order to clearly display the structure of the reagent needle, only a part of the second body portion 611 is shown in fig. 7.

Since the reagent needle 61 needs to contact the sample solution in the reaction container when discharging the reagent, there is also a possibility of cross contamination, and therefore the reagent needle 61 needs to be cleaned after completing the discharge of the reagent to ensure the cleanliness, obviously, the less the reagent remains, the more convenient the subsequent cleaning step. Based on this, the size of the outer diameter of the second needle tip portion 612 is also limited in this embodiment, since the volume of the reagent needle 61 for sucking the reagent once is much larger than the volume of the sample needle 21 for sucking the sample once, the inner diameter and the outer diameter of the second needle tip portion 612 are correspondingly larger than the inner diameter and the outer diameter of the first needle tip portion 212 to adapt to the difference of the flow rates, and the increase of the outer diameter also helps to improve the strength of the reagent needle 61, in this embodiment, the outer diameter D of the second needle tip portion 612 is also limited₂Range of (1)0.7mm to 1.0 mm. This embodiment reduces the outer diameter D of the second needle tip portion 612₂Based on the same principle, when the outer diameter D of the second needle tip portion 612₂When the reagent needle 61 is separated from the reagent liquid surface, the remaining reagent is also reduced, and it is obvious that the present embodiment can further ensure the cleanliness of the reagent needle 61 with the same cleaning capability.

Specifically, two kinds of reagent needles were selected, the tip portions of the two reagent needles were cylindrical, the length L of the reagent needle or the reaction solution inserted into the reagent needle when the tip portions aspirated and discharged the reagent was recorded, the length L was selected to be 5mm, the reagent needle a was an outer diameter size in the related art, the reagent needles b to d were outer diameter sizes in this embodiment, and the outer peripheral surface area S calculated from the above data was shown in table 2, and the outer peripheral surface area S was also reduced in synchronization with the reduction in the outer diameter.

TABLE 2

It should be noted that the second needle tip portion 612 may have any value within the range of 0.7mm to 1.0mm, and is not limited to the values listed in the above table.

The reagent needle 61 of the present embodiment may be cleaned in a non-contact manner to prevent the reagent needle 61 from being bent. Based on this, the sample analysis device is further provided with a reagent needle cleaning mechanism to eliminate the wiping step by the user, which may include a reagent needle ultrasonic cleaning mechanism 110, see fig. 1. In some embodiments, the sample analysis apparatus includes two ultrasonic cleaning mechanisms, that is, the above-described sample needle ultrasonic cleaning mechanism 40 and reagent needle ultrasonic cleaning mechanism 110, the sample needle ultrasonic cleaning mechanism 40 is disposed on a movement locus of the sample needle 21, the reagent needle ultrasonic cleaning mechanism 110 is disposed on a movement locus of the reagent needle 61, the sample needle ultrasonic cleaning mechanism 40 ultrasonically cleans the sample needle 21 when ultrasonic cleaning conditions are satisfied, and the reagent needle ultrasonic cleaning mechanism 110 ultrasonically cleans the reagent needle 61 when the ultrasonic cleaning conditions are satisfied. Of course, the sample needle ultrasonic cleaning mechanism 40 and the reagent needle ultrasonic cleaning mechanism 110 may be the same ultrasonic cleaning mechanism for ultrasonically cleaning the sample needle 21 and the reagent needle 61, and the ultrasonic cleaning mechanism is provided at the intersection of the movement traces of the sample needle 21 and the reagent needle 61.

In some embodiments, referring to fig. 1, the reagent needle washing mechanism may also include a reagent needle normal washing mechanism 70 for performing normal mode washing of the reagent needle 61. The reagent needle normal washing mechanism 70 is used for washing operations such as washing and/or soaking of the reagent needle 61 with a washing liquid and/or a washing agent or the like. The reagent needle general cleaning mechanism 70 may be implemented using existing or future cleaning structures. In some embodiments, the reagent needle normal washing mechanism 70 is disposed on a movement track of the reagent needle 61 so that the reagent needle 61 can be moved to the reagent needle normal washing mechanism 70 for washing.

In some embodiments, referring to fig. 1, the reagent needle cleaning mechanism may include the reagent needle ultrasonic cleaning mechanism 110, or may also include the reagent needle normal cleaning mechanism 70, and both the reagent needle ultrasonic cleaning mechanism 110 and the reagent needle normal cleaning mechanism 70 can clean the reagent needle 61.

In some embodiments of the present invention, referring to FIG. 7, the inner diameter d of the second needle tip portion 612₃0.5mm to 0.8mm, since the single aspiration and discharge amount of the reagent needle 61 is much larger than the single discharge amount of the sample needle 21, for example, the single aspiration and discharge amount of the reagent needle 61 can reach 200 μ l, and the single aspiration and discharge amount of the sample needle 21 is only 25 μ l, the aspiration and discharge speed of the reagent needle 61 is larger than that of the sample needle 21, if the inner diameter of the second needle tip portion 612 is too small, the reagent is easily atomized due to too fast drainage speed during the discharge process, and a large number of micro bubbles are formed in the reaction solution, based on this, the second needle tip portion 612 of the present embodiment samples a relatively large inner diameter, which can slow down the flow speed when the reagent is discharged, and reduce the amount of bubbles in the reaction solution.

In some embodiments of the present invention, the length L and the inner diameter d of the second needle tip portion 612 are adjusted₃The ratio of (a) to (b) is defined,the ratio of the two is less than or equal to 10. Similar to the sample needle 21, in order to suck and discharge the reagent, the reagent needle 61 also needs to transmit power by means of a liquid medium, specifically, one end of the second body portion 611, which is away from the second needle tip portion 612, is connected with a reagent needle fluid path assembly, which includes a water tank, a pumping mechanism, a control valve and a syringe, which are sequentially connected by a connecting pipeline, one end of the syringe is connected with the control valve by the connecting pipeline, and the other end of the syringe is connected with the second body portion 611 by the connecting pipeline, when a piston in the syringe reciprocates, water in the pipeline can enter a piston cavity of the syringe or flow out of the piston cavity, so as to form a negative pressure or a positive pressure in the pipeline, and further drive the reagent needle 61 to suck or discharge the reagent, in order to prevent the water in the fluid path assembly from being polluted by the reagent, when the reagent is sucked, an air section also exists between the reagent and the water in the connecting pipeline, that is, a section of air is sucked before reagent is sucked, and it is necessary to avoid the air bubble in the section from being broken during the sucking and discharging of the reagent. Based on the above, if the length of the second needle tip portion 612 is too long, laminar flow is developed sufficiently when the reagent flows in the second needle tip portion 612, and the longer the length, the more sufficient the laminar flow is, the higher the central flow rate is, which is likely to cause bubble collapse, and therefore, the present embodiment needs to limit the length of the second needle tip portion 612 to avoid the phenomenon of significant stratification in the fluid. Note that, the second needle tip portion 612 needs to extend to a certain depth below the liquid surface both at the time of sucking and discharging the reagent, and therefore the length of the second needle tip portion 612 cannot be too short, for example, not less than 5 mm.

Based on the above, in order to simultaneously satisfy the purpose of reducing reagent residue and reducing reagent flow rate, the second needle tip portion 612 is required to have a smaller outer diameter and a larger inner diameter, that is, the wall thickness of the second needle tip portion 612 cannot be too thick, in some embodiments of the present invention, the wall thickness of the second needle tip portion 612 is 0.1mm to 0.2mm, so that the second needle tip portion 612 can satisfy the requirements of both small outer diameter and large inner diameter while ensuring the processing and forming.

In some embodiments of the present invention, to reduce the dilution ratio of the reagent, the inner diameter d of the second body portion 611₄Is 1.4mm to 1.8 mm. Similar to the sample needle 21, the liquid medium for transmitting power enters the second body portion 611, and the liquid medium in the second body portion 611 decreases synchronously with the increase of the reagent, and a residue is formed on the inner wall of the second body portion 611 during the liquid level moving backward.

In order to avoid abrupt change of the tube diameter between the second needle tip portion 612 and the second main body portion 611, in some modified implementations of the reagent needle described above, the reagent needle 61 further includes a second transition portion 613, the inner diameter of the second main body portion 611 is larger than the inner diameter of the second needle tip portion 612, the inner diameter of the second transition portion 613 gradually decreases along the direction from the second main body portion 611 to the second needle tip portion 612, the head end with the smaller inner diameter is connected to the tail end of the second needle tip portion 612, the tail end with the larger inner diameter is connected to the head end of the second main body portion 611, the taper angle β of the inner wall of the second transition portion 613 is less than or equal to 12 °, based on the above knowledge, the reagent in the reagent needle 61 has the characteristics of large flow rate and fast flow rate, if the inner diameter of the reagent needle 61 abruptly changes, or the change amplitude is too large, the bubble is easily broken, therefore, the embodiment employs a relatively small taper angle range, so that the inner diameter of the second transition portion 230 increases with a decreasing amplitude, thereby reducing the change of the flow rate and reducing the probability of breaking the bubbles due to the sudden change of the flow rate. In some embodiments, the outer diameter of the second main body portion 611 is larger than the outer diameter of the second needle tip portion 612, and the outer diameter of the second transition portion 613 is also gradually reduced along the direction from the second main body portion 611 to the second needle tip portion 612, that is, the second transition portion 613 may have a cone structure in the figure, so that the risk of breaking the reagent needle 61 due to sudden change of the outer diameter can be avoided. The taper angle of the outer wall and the inner wall of the second transition portion 613 may be the same, i.e., the wall thickness of the second transition portion 613 is uniform.

Instead of the reagent needle described above, the reagent needle 61 may be a tapered needle, that is, the tip portion of the reagent needle 61 for aspirating and discharging the reagent may be tapered. Specifically, referring to fig. 8, the reagent needle 61 of the present embodiment includes a third body portion 614 and a third needle tip 615, and the tail end of the third needle tip 615 is connected to the third needle tip 615The head end of the main body 614, along the direction from the third main body 614 to the third needle tip 615, the outer diameter of the third needle tip 615 is gradually reduced, i.e. the outer diameter D of the end of the third needle tip 615, i.e. the outer diameter D of the smallest end of the third needle tip₃0.7mm to 0.9mm, and the taper angle γ of the outer wall is 6 ° or less, which also serves the purpose of reducing the area of the outer peripheral surface of the needle tip portion as compared with the cylindrical reagent needle in the related art.

Specifically, two kinds of reagent needles are selected, wherein the needle tip portion of one of the reagent needles is a cylindrical structure, the needle tip portion of the other reagent needle is a conical structure, the length L of the reagent needle or the reaction solution extending into the reagent needle or the reaction solution when the needle tip portion sucks and discharges the reagent is recorded, the length L is selected to be 5mm, the reagent needle a adopts the outer diameter size in the related art, the reagent needles b to f adopt the outer diameter size in the present embodiment, the outer peripheral surface area S calculated according to the above data is shown in table 3, the outer peripheral surface area of the third needle tip portion 615 is smaller than the outer peripheral surface area of the reagent needle a in the related art, and the outer peripheral surface area is correspondingly reduced along with the reduction of the outer diameter size of the minimum end. On the other hand, combining the reagent needles b, e, and f in table 3, it can be seen that the area of the outer peripheral surface of the third needle tip 615 decreases in synchronization with the decrease in the taper angle. To sum up, reducing the minimum end outer diameter and the taper angle of the third needle tip portion 615 can reduce the outer circumferential surface area of the third needle tip portion 615.

TABLE 3

It should be noted that, although the outer peripheral surface area of the third needle tip portion 615 is larger than that of the second needle tip portion 612 of the same specification (combining the reagent needle d in table 2 with the reagent needle c in table 3), it is more favorable for stabilizing the reagent flow rate because: since the inner diameter of the second needle tip portion 612 is constant and smaller than the inner diameter of the second main body portion 611, even if the taper angle of the second transition portion 613 is further reduced, the flow rate of the reagent inevitably changes abruptly when the reagent enters the second transition portion 613 from the second needle tip portion 612, that is, the flow rate of the reagent is suddenly reduced from being substantially constant, and therefore, there is a risk that the separation bubble between the reagent and the power fluid path is broken, and the reagent is diluted. However, the needle tip portion of the reagent needle 61 of the present embodiment does not involve the transition from the cylindrical section to the conical section, and the inner diameter of the large end of the third needle tip portion 615 is equal to the inner diameter of the third main body portion 614, so that the flow rate of the reagent entering the third needle tip portion 615 is gradually reduced and always flows in the third main body portion 614 at a substantially constant flow rate, and no abrupt change of the flow rate occurs in the process, thereby reducing the possibility of the bubble being broken due to the change of the flow rate.

It should be noted that the minimum outer diameter and taper angle of the third needle tip portion 615 can be any value within the above range, and are not limited to the values listed in the above table.

In some modified implementations of the reagent needle described above, the inner diameter d of the third body portion 614₅1.4mm to 1.8mm, and the principle of using this size range is the same as that of the second body portion 611, and will not be described in detail.

In another embodiment of the present invention, another analyzing apparatus is disclosed, the sample dispensing mechanism of the analyzing apparatus includes a sample needle 21 having a tapered tip, and referring to fig. 9, the sample needle 21 of this embodiment includes a fourth body portion 214 and a fourth tip portion 215, an outer diameter of the fourth body portion 214 is larger than an outer diameter of the fourth tip portion 215, a trailing end of the fourth tip portion 215 is connected to a leading end of the fourth body portion 214, and an outer diameter of the fourth tip portion 215 is gradually reduced in a direction from the fourth body portion 214 to an end of the fourth tip portion 215, that is, a tapered structure, wherein an outer diameter D of an end portion, that is, a smallest end of the fourth tip portion 215 is a smallest diameter₄0.45mm to 0.6mm, and the taper angle δ of the outer wall is 6 ° or less, which also serves the purpose of reducing the area of the outer peripheral surface of the needle tip portion as compared with the cylindrical sample needle in the related art.

Specifically, two kinds of sample needles are selected, wherein the tip portion of one sample needle is a cylindrical structure, the tip portion of the other sample needle is a conical structure, the length L of the tip portion extending into the sample during reagent suction and discharge is recorded, the length L is selected to be 5mm, the sample needle a adopts the outer diameter size of the related art, the sample needles b to e adopt the outer diameter size of the embodiment, the outer peripheral surface area S calculated according to the data is shown in table 4, the outer peripheral surface area of the fourth tip portion 215 is smaller than that of the sample needle a of the related art, and the outer peripheral surface area is correspondingly reduced along with the reduction of the outer diameter size of the smallest end. On the other hand, in combination of the sample needle b and the sample needle e in table 4, it can be seen that as the taper angle decreases, the area of the outer peripheral surface of the fourth needle-tip portion 215 also decreases synchronously. To sum up, reducing the minimum end outer diameter and the taper angle of the fourth needle tip portion 215 can reduce the outer circumferential surface area of the fourth needle tip portion 215.

TABLE 4

The analyzing apparatus also includes the above-described sample dispensing mechanism 20, sample mechanism 10, reaction mechanism 80, measurement mechanism 100, reagent dispensing mechanism 60, reagent mechanism 50, sample needle ultrasonic cleaning mechanism 40, and liquid path support mechanism, so that the sample needle 21 of the present embodiment can be cleaned in a non-contact manner by the ultrasonic cleaning mechanism as well, and the outer diameter of the needle tip is reduced, and the needle tip is prevented from being damaged by bending or the like during wiping.

In some embodiments of the present invention, the inner diameter of the end of the fourth needle tip portion 215 is 0.29mm to 0.33mm, and similar to the above embodiments, this embodiment can increase the flow resistance of the sample flowing in the fourth needle tip portion 215 by setting a smaller inner diameter, so that when the flow rate and the flow rate of the liquid medium in the pipeline are changed due to the elastic deformation of the pipeline, the affected amplitude of the sample liquid will be correspondingly reduced because the sample liquid flowing in the fourth needle tip portion 215 needs to overcome a relatively large flow resistance, thereby ensuring the sampling accuracy of the sample needle 21 to a certain extent.

In other embodiments of the present invention, another analysis apparatus is disclosed, the analysis apparatus includes the above-mentioned sample dispensing mechanism 20, sample mechanism 10, reaction mechanism 80, measurement mechanism 100, reagent dispensing mechanism 60, and reagent mechanism 50, the sample dispensing mechanism 20 includes a sample needle 21, and the sample needle 21 further reduces the residue of the sample by adding a coating. Specifically, the sample needle 21 of the present embodiment includes a fifth main body portion and a fifth needle tip portion, the outer diameter of the fifth main body portion is larger than the outer diameter of the fifth needle tip portion, the tail end of the fifth needle tip portion is connected to the head end of the fifth main body portion, and both of the fifth main body portion and the fifth needle tip portion are cylindrical structures, that is, the sample needle 21 of the present embodiment may be the same as or similar to the sample needle 21 shown in fig. 6. The fifth needle tip portion has an outer diameter of 0.45mm to 0.6mm, and the sample needle of the present embodiment has a smaller needle tip portion than the sample needle in the related art, so that the area of the outer circumferential surface of the needle tip portion can be reduced, thereby reducing the attachment area of the sample.

On the other hand, the outer surface of the fifth needle tip portion is provided with the polymer coating, and the contact angle of the polymer coating is larger than 90 degrees, namely the polymer coating presents hydrophobic property, so that the residue of the sample liquid can be further reduced, and the subsequent cleaning is facilitated. Of course, the inner surface of the fifth needle tip may also be provided with a polymer coating.

In this embodiment, non-contact cleaning may be performed by using an ultrasonic cleaning mechanism.

In some embodiments of the utility model, the contact angle of the polymer coating may further be greater than or equal to 120 °, the greater the contact angle, the stronger the hydrophobic properties of the coating. Specifically, the polymer coating can adopt a teflon coating, has excellent hydrophobicity and stability, and can avoid reaction with the sample liquid to influence the detection result while reducing the liquid hanging.

In still another embodiment of the present invention, another analyzing apparatus is disclosed, the analyzing apparatus includes the above-mentioned sample dispensing mechanism 20, the sample mechanism 10, the reaction mechanism 80, the measurement mechanism 100, the reagent dispensing mechanism 60 and the reagent mechanism 50, the sample dispensing mechanism 20 includes the sample needle 21, the sample needle 21 of the present embodiment includes a sixth body portion and a sixth needle tip portion, the outer diameter of the sixth body portion is larger than the outer diameter of the sixth needle tip portion, the trailing end of the sixth needle tip portion is connected to the leading end of the sixth body portion, and the outer diameter of the sixth needle tip portion is gradually reduced in a direction from the sixth body portion to the end of the sixth needle tip portion, that is, the sample needle 21 of the present embodiment may be the same as or similar to the sample needle 21 shown in fig. 9. The outer diameter of the end of the sixth needle tip portion 190 is 0.45mm to 0.6mm, the taper angle of the outer wall is less than or equal to 6 °, and compared with a sample needle in the related art, the sample needle of the present embodiment has a smaller needle tip portion, so that the area of the outer peripheral surface of the needle tip portion can be reduced, and the adhesion area of a sample can be further reduced.

In this embodiment, the outer surface of sixth needle point portion also is provided with the polymer coating, and the contact angle of polymer coating is greater than 90 °, shows hydrophobic property promptly, can further reduce the residue of sample liquid, is convenient for subsequent washing. Of course, the inner surface of the sixth needle tip may also be provided with a polymer coating.

The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge of those skilled in the art without departing from the gist of the present invention. Furthermore, the embodiments of the present invention and the features of the embodiments may be combined with each other without conflict.

Claims

1. A sample analysis apparatus, comprising:

2. The sample analysis device of claim 1, wherein the first needle tip has an inner diameter of 0.25mm to 0.4 mm.

3. The sample analysis device of claim 1, wherein the first body portion has an inner diameter of 0.6mm to 0.8 mm.

4. The sample analysis apparatus of claim 1, wherein the sample needle further comprises a first transition portion connected between the first needle tip portion and the first body portion;

5. The sample analysis apparatus of claim 1, further comprising:

the reagent mechanism is provided with at least one reagent position, the reagent position is used for placing a reagent container, the reagent container is used for accommodating a reagent, and the reaction liquid is prepared by the sample and the reagent;

6. The sample analysis device of claim 5, wherein the second needle tip portion has an inner diameter of 0.5mm to 0.8 mm.

7. The sample analysis apparatus of claim 5, wherein the second needle tip portion has a length to inner diameter ratio less than or equal to 10.

8. The sample analysis device of claim 5, wherein the second body portion has an inner diameter of 1.4mm to 1.8 mm.

9. The sample analysis device of claim 5, wherein the reagent needle further comprises a second transition portion connected between the second tip portion and the second body portion;

10. The sample analysis apparatus of claim 1, further comprising:

11. The sample analysis device of claim 10, wherein the third body portion has an inner diameter of 1.4mm to 1.8 mm.

12. The sample analysis apparatus of claim 1, wherein the ultrasonic sound source assembly is located at a bottom of the wash tank to transmit sound field vibrations through a bottom wall of the wash tank to the wash liquid within the wash tank.

13. The sample analysis apparatus according to claim 12, wherein the wash basin is provided with a wash chamber into which the sample needle is inserted and an overflow chamber for receiving the wash liquid overflowing from the wash chamber.

14. A sample analysis apparatus, comprising:

a sample dispensing mechanism including a first moving member and a sample needle provided on the first moving member, the first moving member being configured to drive the sample needle to move between the sample position and the reaction position to suck a sample at the sample position and discharge the sample at the reaction position by the sample needle, the sample needle including a fourth body portion and a fourth tip portion, the fourth tip portion being connected to the fourth body portion, an outer diameter of the fourth body portion being larger than an outer diameter of the fourth tip portion, the outer diameter of the fourth tip portion being gradually reduced in a direction from the fourth body portion to an end of the fourth tip portion, wherein the outer diameter of the end of the fourth tip portion is 0.45mm to 0.6mm, and a taper angle of an outer wall of the fourth tip portion is less than or equal to 6 °;

a measuring mechanism for measuring a reaction solution to be measured;

15. The sample analysis device of claim 14, wherein an inner diameter of an end of the fourth needle tip portion is 0.29mm to 0.33 mm.

16. A sample analysis apparatus, comprising:

and a measuring mechanism for measuring the reaction solution to be measured.

17. A sample analysis apparatus, comprising:

a sample dispensing mechanism including a first moving member and a sample needle provided on the first moving member, the first moving member for driving the sample needle to move between the sample position and the reaction position to aspirate a sample at the sample position and discharge the sample at the reaction position through the sample needle, the sample needle including a sixth body portion and a sixth needle tip portion, the sixth needle tip portion being connected to the sixth body portion, the sixth body portion having an outer diameter larger than an outer diameter of the sixth needle tip portion, the sixth needle tip portion having an outer diameter gradually reduced in a direction from the sixth body portion to an end of the sixth needle tip portion, wherein the outer diameter of an end of the sixth needle tip portion is 0.45mm to 0.6mm, and a taper angle of an outer wall of the sixth needle tip portion is 6 ° or less, and at least an outer surface of the sixth needle tip portion is provided with a polymer coating layer, the contact angle of the polymer coating is greater than 90 °;

and a measuring mechanism for measuring the reaction solution to be measured.

18. The sample analysis device of claim 16 or 17, wherein the polymer coating has a contact angle greater than or equal to 120 °.