CN216890949U - Water supply pipe microbiological reaction simulation device - Google Patents

Abstract

The utility model provides a microbial reaction simulation device for a water supply pipe, which comprises a rectangular pipe, two ends of the rectangular pipe are respectively connected with a water inlet pipe and a water outlet pipe, the water inlet pipe is provided with a pressurized water pump and a valve group; There are a plurality of installation grooves connected to the inside of the installation groove, each installation groove is disassembled and connected with a sterile glass sheet, and the lower end surface of each sterile glass sheet is flush with the top inner surface of the rectangular tube; Under the action of the pressure pump, it enters the rectangular tube at a certain initial speed through the water inlet pipe. After a period of time, the tube is full, and the water flows out of the rectangular tube from the water outlet pipe. After a period of reaction time, a microbial community will be formed on the sterile glass sheet. The bacterial glass sheet can obtain the microbial community that can be used for experimental analysis. Since the water supply pipeline microbial reaction simulation device in this scheme has no built-in filler in the rectangular tube, it will not affect the water flow state, and can accurately simulate the type and quantity of the microbial community in the actual water pipeline. .

Description

A microbial reaction simulation device for water supply pipeline

technical field

The utility model relates to the technical field of environmental microbiology, in particular to a microbial reaction simulation device for a water supply pipeline.

Background technique

At present, most of the microbial community growth simulation devices in the prior art maintain a certain water flow, and place fillers in the simulation device to increase the adhesion area of microorganisms, so that more microorganisms in the water are trapped on the sampling device. However, such a simulation device fails to take into account the shape and hydraulic conditions of the actual water supply pipe network. In addition, the placed filler will inevitably affect the water flow state in the device. Previous studies have shown that hydraulic conditions such as water flow velocity in the water supply pipe, shear stress of the water flow near the pipe wall, and static pressure will affect the microbial community growing on the pipe wall. Because most of the reported simulation devices fail to simulate the hydraulic conditions in the actual pipeline network, it is difficult to ensure that the type and quantity of the water body in the simulation device and the collected microorganisms are close to the actual situation.

Utility model content

In view of the above problems in the prior art, the present invention aims to provide a water supply pipeline microbial reaction simulation device, which solves the problem that the microbial community growth simulation device cannot accurately simulate the microbial community in the actual water pipeline because the built-in filler affects the water flow state. kind and quantity issues.

In order to achieve the above-mentioned purpose of the invention, the technical scheme adopted by the utility model is as follows:

Provided is a microbial reaction simulation device for a water supply pipeline, which comprises a rectangular tube, two ends of the rectangular tube are respectively connected with a water inlet pipe and a water outlet pipe, and a pressurized water pump and a valve group are arranged on the water inlet pipe;

The top outer surface of the rectangular tube is provided with a plurality of installation grooves that are communicated with the inside thereof, each installation groove is disassembled and connected with a piece of sterile glass sheet, and the lower end surface of each piece of the sterile glass sheet is connected with the top of the rectangular tube. The inner surface is flush.

The basic principle of the microbial reaction simulation device for the water supply pipeline in this scheme is as follows: open the valve group, and the water enters the rectangular tube at a certain initial speed through the water inlet pipe under the action of the pressurized water pump. After a period of reaction time, a microbial community will be formed on the sterile glass slide, and the microbial community that can be used for experimental analysis can be obtained by directly removing the sterile glass slide. At the same time, the sterile glass sheets are directly sterilized in a 120°C high temperature sterilization pot before use, which can ensure that the collected microbial community microorganisms are generated during the water supply process of the simulated device, and they can be sterilized again after use and can be reused; The microbial reaction simulation device for the water supply pipeline in this scheme has no built-in filler in the rectangular pipe, which will not affect the water flow state, and can accurately simulate the type and quantity of the microbial community in the actual water pipe.

Further, the number of the installation grooves is 4, and the 4 installation grooves are evenly arranged at 0.4m to 1.2m in the length direction of the rectangular pipe. Compared with the round tube, do you need to explain? There are aseptic glass sheets installed in the 4 installation slots, which can collect samples of multiple types and quantities of microbial communities, which can not only improve the collection efficiency, but also obtain multiple sterile glass sheets with attached microbial communities. More prepared and comprehensive simulation of the type and quantity of microorganisms in the effluent pipe; at the same time, the sterile glass sheet is set at the position of 0.4m to 1.2m in the rectangular pipe, and the shear stress of the pipe wall and the state of water flow at the position of 0.4m to 1.2m of the rectangular pipe. For stability, it can reduce the impact of pipe wall shear stress on the microbial community on the glass slide, and ensure that the microbial community generated by the entire microbial reaction simulation device is consistent with the actual water supply network.

Further, as a specific design method of the rectangular tube and the sterile glass sheet, the size of the rectangular tube is 15000×40×30mm, the size of each piece of the sterile glass sheet is 760×260×1mm, and the installation groove is the same length as the sterile glass slide. Due to the need to install sterile glass sheets, the pipe needs to be set as a rectangular pipe, but the rectangular pipe will affect the water flow inside it. In order to simulate the hydraulic conditions in the real pipe network, the design size of the rectangular pipe is simulated and determined by CFD calculation. The size of the rectangular tube is 15000 × 40 × 30mm, so that the water flow in the rectangular tube is a non-circulating flow state, which can truly simulate the water flow state in the pipeline.

Further, as a specific connection method between the sterile glass sheet and the installation groove, each of the four corners of the sterile glass sheet is provided with a mounting hole, and each of the mounting holes is provided with a threaded connector, and the thread The connecting piece includes a screw rod and a hand-held wrench fixedly arranged on the top of the screw rod; the setting of the hand-held wrench is convenient for the experimenter to twist the screw rod by hand without touching the tool, thereby improving the installation and removal efficiency of the sterile glass sheet. The bottom of the installation slot is provided with 4 threaded holes matching the positions of the 4 installation holes, the depth of the threaded holes is greater than the length of the screw; The lower surface of the hand-held wrench is in close contact with the upper end surface of the sterile glass slide.

Further, a rubber gasket is provided on the lower surface of the hand-held wrench. By twisting the hand-held wrench, the screw is moved downward until the lower end surface of the hand-held wrench is in contact with the rubber gasket, so that the rubber gasket is deformed and then stops. The screw is loose to ensure that the sterile glass sheet is well fixed; at the same time, the rubber gasket can buffer the force of the hand-held wrench on the sterile glass sheet, which can prevent the sterile glass sheet from being crushed by the hand-held wrench.

Further, a sealing gasket is provided on the contact surface between the lower surface of the sterile glass sheet and the installation groove; the sealing gasket can prevent the water in the rectangular tube from overflowing from the gap between the sterile glass sheet and the installation groove.

The beneficial effects of the present invention are as follows: 1. Since the water supply pipeline microbial reaction simulation device in this scheme has no built-in filler in the rectangular tube, the water flow state will not be affected, and the type and quantity of the microbial community in the actual water pipeline can be accurately simulated.

2. When sampling the microbial reaction simulation device of the water supply pipeline in this scheme, it is only necessary to directly remove the sterile glass piece fixed on the upper pipe wall. The sampling process does not affect the microbial environment in the simulation device, and the size of the sterile glass piece can be adjusted. It is directly used for the direct analysis of laboratory microscopes and point spray desorption ionization instruments without additional transfer, avoiding possible interference during the transfer of microbial communities, and ensuring the quality of microbial samples.

Description of drawings

FIG. 1 is a schematic structural diagram of a microbial reaction simulation device for a water supply pipeline.

Fig. 2 is a schematic diagram of the structure of the sterile glass sheet disposed in the installation groove.

FIG. 3 is a schematic top view of the structure of the installation slot provided on the rectangular tube.

Figure 4 is a schematic structural diagram of a sterile glass sheet.

Among them, 1. Rectangular pipe; 2. Inlet pipe; 3. Outlet pipe; 4. Pressurized water pump; 5. Valve group; 6. Installation groove; 7. Aseptic glass sheet; 8. Installation hole; 9. Screw; 10 , Hand wrench; 11, threaded hole; 12, rubber gasket; 13, gasket.

Detailed ways

The specific embodiments of the present invention are described below to facilitate those skilled in the art to understand the present invention, but it should be clear that the present invention is not limited to the scope of the specific embodiments. Various changes are within the spirit and scope of the present utility model defined and determined by the appended claims, and these changes are obvious, and all inventions and creations utilizing the concept of the present utility model are included in the protection list.

As shown in Figures 1 to 4, the utility model provides a water supply pipeline microbial reaction simulation device, which comprises a rectangular tube 1, and the two ends of the rectangular tube 1 are respectively connected with a water inlet pipe 2 and a water outlet pipe 3, The water inlet pipe 2 is provided with a pressurized water pump 4 and a valve group 5 . The model of the pressurized water pump 4 can be a JWS-BL horizontal variable frequency water pump, and the valve group 5 can be a solenoid valve water valve.

The top outer surface of the rectangular tube 1 is provided with a plurality of installation grooves 6 communicating with its interior, and a piece of sterile glass sheet 7 is disassembled and connected in each installation groove 6, and the lower end face of each described sterile glass sheet 7 is Flush with the top inner surface of the rectangular tube 1.

The basic principle of the water supply pipeline microbial reaction simulation device in this scheme is: open the valve group 5, and the water enters the rectangular pipe 1 through the water inlet pipe 2 at a certain initial speed under the action of the pressurized water pump 4. After a period of time, the pipe is full, and the water The rectangular tube 1 flows out from the water outlet pipe 3. After a period of reaction time, a microbial community will be formed on the sterile glass sheet 7, and the sterile glass sheet 7 can be directly removed to obtain a microbial community that can be used for experimental analysis. At the same time, the sterile glass sheet 7 is directly sterilized in a 120°C high-temperature sterilization pot before use, which can ensure that the collected microbial community microorganisms are generated during the water supply process of the simulated device, and are sterilized after use and can be reused; Since the water supply pipeline microbial reaction simulation device in this scheme has no built-in filler in the rectangular pipe 1, it will not affect the water flow state, and the type and quantity of the microbial community in the actual water pipe can be accurately simulated.

When sampling the water supply pipeline microbial reaction simulation device, it is only necessary to directly remove the sterile glass sheet 7 fixed on the upper pipe wall. The sampling process does not affect the microbial environment in the simulation device, and the size of the sterile glass sheet 7 can be directly used for experiments. The direct analysis of the chamber microscope and the point spray desorption ionization instrument does not require another transfer, which avoids possible interference during the transfer of the microbial community and ensures the quality of the microbial samples.

Preferably, but not limitedly, the number of the installation grooves 6 is 4, and the four installation grooves 6 are evenly spaced at 0.4m to 1.2m in the length direction of the rectangular tube 1, and within the four installation grooves 6 All are equipped with sterile glass sheets 7, which can collect samples of multiple types and quantities of microbial communities, which can not only improve the collection efficiency, but also obtain multiple sterile glass sheets 7 with attached microbial communities, which can be more prepared and comprehensively simulated. The type and quantity of microorganisms in the 3 channels of the water outlet pipe; at the same time, the sterile glass sheet 7 is set at the position of 10.4m to 1.2m of the rectangular pipe, and the shear stress and water flow state of the pipe wall at the position of 10.4m to 1.2m of the rectangular pipe tend to be stable, and can Reduce the impact of pipe wall shear stress on the microbial community on the glass slide, and ensure that the microbial community generated by the entire microbial reaction simulation device is consistent with the actual water supply network.

Since the sterile glass sheet 7 needs to be installed, the pipe needs to be set as a rectangular pipe 1, preferably but not limitedly, the size of the rectangular pipe 1 is 15000×40×30mm, the rectangular pipe 1 will affect the water flow inside it, in order to simulate the real The hydraulic conditions in the pipe network, the design size of the rectangular pipe 1 is simulated by simulation, and the size of the rectangular pipe 1 is determined to be 15000 × 40 × 30mm through CFD calculation, so that the water flow in the rectangular pipe 1 is a non-circulating flow state, which can truly simulate the pipeline In the state of medium water flow, the size of each sterile glass piece 7 is 760×260×1 mm.

As a specific connection method between the sterile glass sheet 7 and the mounting groove 6, each of the four corners of the sterile glass sheet 7 is provided with a mounting hole 8, and each of the mounting holes 8 is provided with a threaded connector , the threaded connector includes a screw 9 and a hand-held wrench 10 fixed on the top of the screw 9; the setting of the hand-held wrench 10 is convenient for the experimenter to manually twist the screw 9 without touching the tool, improving the installation and removal efficiency of the sterile glass sheet 7. The bottom of the mounting slot 6 is provided with four threaded holes 11 that match the positions of the four mounting holes 8, and the depth of the threaded holes 11 is greater than the length of the screw 9; the bottom of the screw 9 passes through the mounting holes 8 and The threaded hole 11 is threadedly connected, and the lower surface of the hand-held wrench 10 is in close contact with the upper end surface of the sterile glass sheet 7 .

The lower surface of the hand-held wrench 10 is provided with a rubber gasket 12 . The screw 9 is moved downward by twisting the hand-held wrench 10 until the lower end surface of the hand-held wrench 10 is in contact with the rubber gasket 12, so that the rubber gasket 12 is deformed and then stopped. After the rubber gasket 12 is deformed, a hand-held wrench 10 will be given The upward force can prevent the screw 9 from loosening and ensure that the sterile glass sheet 7 is well fixed; at the same time, the rubber gasket 12 can buffer the force of the hand-held wrench 10 on the sterile glass sheet 7, which can prevent the sterile glass sheet 7 from being held by hand. Wrench 10 crushed case.

A sealing gasket 13 is provided on the contact surface between the lower surface of the sterile glass sheet 7 and the installation groove 6; Filling the gap between the sterile glass sheet 7 and the bottom surface of the mounting groove 6 , the gasket 13 can prevent the water in the rectangular tube 1 from overflowing from the gap between the sterile glass sheet 7 and the mounting groove 6 .

Claims (6)

1. A microbial reaction simulation device for a water supply pipeline is characterized by comprising a rectangular pipe, wherein a water inlet pipe and a water outlet pipe are respectively connected to two ends of the rectangular pipe, and a pressure water pump and a valve group are arranged on the water inlet pipe;

be provided with a plurality of mounting grooves rather than inside intercommunication on the top surface of rectangular pipe, all dismantle in every mounting groove and be connected with a aseptic glass piece, every the lower terminal surface of aseptic glass piece all with the top internal surface parallel and level of rectangular pipe.

2. The microbial reaction simulation device for the water supply pipeline according to claim 1, wherein the number of the installation grooves is 4, and the 4 installation grooves are uniformly arranged at the positions of 0.4-1.2 m in the length direction of the rectangular pipe at intervals.

3. The microbial reaction simulation device for the water supply pipeline according to claim 2, wherein the size of the rectangular pipe is 15000 x 40 x 30mm, the size of each sterile glass sheet is 760 x 260 x 1mm, and the length of the installation groove is the same as that of the sterile glass sheet.

4. The microbial reaction simulation device for the water supply pipeline according to claim 3, wherein a mounting hole is formed in each of four corners of each sterile glass sheet, a threaded connecting piece is arranged in each mounting hole, and each threaded connecting piece comprises a screw and a handheld wrench fixedly arranged at the top of the screw;

the bottom of the mounting groove is provided with 4 threaded holes matched with the 4 mounting holes in position, and the depth of each threaded hole is greater than the length of each screw rod;

the bottom of the screw rod penetrates through the mounting hole to be in threaded connection with the threaded hole, and the lower surface of the handheld wrench is in close contact with the upper end face of the sterile glass sheet.

5. The microbial reaction simulation device for the water supply pipeline according to claim 4, wherein a rubber gasket is arranged on the lower surface of the hand-held wrench.

6. The microbial reaction simulation device for the water supply pipeline according to claim 5, wherein a sealing gasket is arranged on a contact surface of the lower surface of the sterile glass sheet and the mounting groove.

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