CN216036354U - A transportation save set for peripheral blood DNA methylation detects - Google Patents

Abstract

The utility model belongs to the field of reagent storage devices, and particularly relates to a transportation and storage device for peripheral blood DNA methylation detection. The utility model can effectively preserve the quality of the reagent for a long time and overcome the problems of vibration and leakage in the transportation process.

Description

A transportation save set for peripheral blood DNA methylation detects

Technical Field

The utility model belongs to the field of reagent storage devices, and particularly relates to a transportation and storage device for peripheral blood DNA methylation detection.

Background

DNA methylation is an important mechanism for gene expression regulation, and DNA methylation detection refers to the determination of the degree of DNA methylation in tumor cells by various methods. In the development of malignant tumor, the methylation state is not invariable, the hypomethylation degree of the whole genome in the tumor cell has a close relation with the disease progression, the tumor size and the malignancy degree, and the DNA methylation detection has an important meaning for judging the malignancy degree of the tumor.

The accuracy of the test is very high for researchers, and therefore, the preservation and transportation of the sample to be tested becomes the most critical link. Reagent bottle is sealed badly, leads to reagent to spill over under vibrations or the jolt environment, and the reagent in the reagent bottle contacts outside air for a long time, and then leads to reagent to take place the oxidation, seriously influences the precision that detects requirement and testing result. Generally, reagent bottles need to be stored in a specified low-temperature environment, and a common cooling device is complex in structure, high in energy consumption, short in maintaining time of refrigeration and heat preservation effects and poor in long-term storage capacity. Therefore, a transport and storage device for peripheral blood DNA methylation detection is needed.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a transportation and storage device for peripheral blood DNA methylation detection, so as to solve the problems.

In order to achieve the purpose, the utility model provides the following scheme: a transportation and preservation device for peripheral blood DNA methylation detection comprises a box body, wherein a box cover is hinged to the long edge of the top surface of the box body, a buffering heat-insulating body is arranged in the box cover and the box body, a plurality of reagent bottles are placed in the buffering heat-insulating body, a cooling mechanism for maintaining the low temperature of the reagent bottles is arranged at the bottom of the inner side of the box body, and four corners of the bottom of the outer side of the box body are respectively provided with a damping support;

the buffering heat-preservation body comprises a fixed connection and is in the case cover is close to a first heat-preservation main body and a fixed connection on one side of the case body are in a second heat-preservation main body on the inner side of the case body, the first heat-preservation main body is matched with the top surface of the second heat-preservation main body, the first heat-preservation main body is close to a plurality of limiting blocks fixedly connected on one side of the case body, the reagent bottle is arranged in the second heat-preservation main body, the limiting blocks correspond to the reagent bottle, the reagent bottle is located above the cooling mechanism, and the reagent bottle is used for hermetically preserving peripheral blood DNA methylation reagents.

Preferably, the reagent bottle is including pegging graft the internal test tube of second heat preservation main part, the mouth of pipe of test tube is provided with sealed lid, sealed inboard keeping away from the one end fixedly connected with sealing plug main part at the bottom of the test tube, the mouth of pipe of test tube is pegged graft the sealing plug main part with between the sealed lateral wall of covering, the test tube with be provided with the rubber seal cover between the sealing plug main part, the rubber seal cover cup joints on the sealing plug main part lateral wall, sealing plug main part center is provided with and is used for discharging the inside gaseous exhaust portion of test tube.

Preferably, the exhaust portion is including seting up the constant voltage passageway at sealing plug main part center, the constant voltage passageway is kept away from the inboard grafting of one end of test tube has and is used for sealing the shutoff of constant voltage passageway, the constant voltage passageway is close to the one end intercommunication of test tube has seted up the sealed piece activity chamber at sealing plug main part center, sealed piece activity chamber is kept away from the one end of constant voltage passageway has seted up the exhaust hole, sealed piece activity intracavity side is provided with the exhaust floating block, the exhaust floating block is close to one side in exhaust hole with exhaust hole looks adaptation.

Preferably, the edge of the exhaust floating block is in sliding connection with the inner side wall of the movable cavity of the sealing block, a plurality of air holes are formed in the edge of the exhaust floating block at equal intervals in the circumferential direction, a circular truncated cone structure is arranged below the exhaust floating block, a protrusion is arranged on one side, away from the exhaust floating block, of the circular truncated cone structure, and the protrusion is matched with the air holes.

Preferably, second heat preservation main part top surface seted up with the recess of first heat preservation main part looks adaptation, the grooved underside is provided with places the layer, it is used for placing a plurality of to offer on the layer to place a plurality of chambeies of accomodating of reagent bottle, it is provided with the test tube and places the chamber to accomodate the intracavity, place and seted up the spacing groove on the layer, the spacing groove sets up the test tube place the chamber top and with the test tube places the chamber intercommunication, the spacing groove with stopper looks adaptation, the test tube is placed the intracavity and is provided with and is used for fixing the fixed establishment of reagent bottle, the stopper with the fixed establishment butt.

Preferably, the fixing mechanism comprises a chute and a sealing cover plate which are arranged at the bottom of the test tube placing cavity, the chute is arranged at one end close to the bottom of the test tube, the sealing cover plate is arranged at one end close to the sealing cover, a fixing rod is fixedly connected in the chute, one end of the fixing rod far away from the bottom of the test tube is fixedly connected at one end of the sealing cover plate far away from the sealing cover, one end of the fixing rod close to the bottom of the test tube is fixedly connected to one side surface of the chute close to the bottom of the test tube, the central line of the fixing rod is parallel to the central line of the test tube, a sliding block is slidably connected to the outer side of the fixing rod, the outer side of the sliding block is slidably connected in the chute, a first spring is arranged between the sliding block and the second heat preservation main body, the first spring is sleeved on the outer side of the fixing rod, and a test tube limiting shell is hinged above the sliding block, the at the bottom of the test tube is pegged graft the spacing casing of test tube is inboard, the test tube transversely sets up the intracavity side is placed to the test tube, sealed lid is kept away from the one end of test tube with the intracavity lateral wall butt is placed to the test tube, the stopper with the spacing casing lateral wall butt of test tube.

Preferably, the spacing casing of test tube with the articulated shaft outside of slider has cup jointed the torsional spring, it is provided with a plurality of archs in the layer to place, protruding bottom with sealed side butt of covering, the arch is located sealed lid is kept away from the one end top at the bottom of the test tube.

Preferably, damping support is including seting up the annular cavity of bottom half, annular cavity center is provided with the support column, support column fixed connection be in the bottom half, support column bottom diameter is greater than support column top diameter, support column bottom outside spiro union has the rotation regulation cover, the rotation regulation cover is located in the annular cavity, support column bottom surface fixedly connected with slides telescopic link one end, slide telescopic link other end fixedly connected with cushion, the support column bottom surface with be provided with the second spring between the cushion, just the second spring sets up the slide telescopic link outside.

Preferably, the cooling mechanism comprises a liquid nitrogen dewar arranged at the bottom of the inner side of the box body, one end of the liquid nitrogen dewar, which is close to the side wall of the box body, is provided with a liquid inlet, one end of the liquid nitrogen dewar, which is far away from the liquid inlet, is communicated with one end of the liquid outlet, the other end of the liquid outlet is communicated with the bottom end of a second cooling cavity, the top end of the second cooling cavity is communicated with a first cooling cavity through a nitrogen channel, the first cooling cavity is connected with a pressure release valve, and the first cooling cavity is positioned below the reagent bottle; the reagent bottle cooling device is characterized in that a low-temperature conducting layer is arranged on the top of the first cooling cavity, a heat insulating layer is arranged at the bottom of the first cooling cavity, the low-temperature conducting layer is located between the reagent bottle and the top of the first cooling cavity, and the heat insulating layer is located between the bottom of the first cooling cavity and the second cooling cavity.

Preferably, a gap layer is formed between the first heat preservation body and the placing layer, and the gap layer is communicated with the first cooling cavity through a ventilation channel.

The utility model has the following technical effects: the box cover and the box body are mainly used for storing reagent bottles, and the reagent bottles have good sealing effect; the cooling mechanism is mainly used for providing a continuous low-temperature storage environment for the reagent bottle, prolonging the storage period and maintaining the quality of the reagent; the main functions of the buffer heat-insulating body are that on one hand, the influence of external vibration on the reagent bottle can be avoided, and on the other hand, the heat insulation can be carried out on the reagent bottle; the first heat preservation main part and the limiting block are beneficial to fixing the reagent bottle in the second heat preservation main part.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

FIG. 1 is a schematic view of a transportation and storage unit;

FIG. 2 is a vertical sectional view of a reagent bottle;

FIG. 3 is a schematic view of the exhaust float;

FIG. 4 is a schematic view of the lateral placement of a reagent bottle;

FIG. 5 is a front sectional view of the shock mount;

FIG. 6 is a front sectional view of the cooling mechanism;

FIG. 7 is a cross-sectional view of an empty test tube placement chamber;

FIG. 8 is a sectional view of a shock absorbing mount according to a second embodiment in a front view;

wherein, 1, a box cover; 2. a box body; 3. a shock-absorbing support; 4. a first thermal insulation body; 5. a limiting block; 6. a second insulation main body; 601. placing the layer; 7. a reagent bottle; 8. a liquid nitrogen dewar flask; 9. a liquid inlet; 10. a liquid outlet; 11. a vent passage; 12. a test tube; 13. a sealing cover; 14. a sealing plug body; 15. a rubber sealing sleeve; 16. a constant pressure channel; 17. plugging; 18. A sealing block active cavity; 19. sealing the floating block; 20. air holes are formed; 21. an exhaust hole; 22. a test tube limiting shell; 23. a test tube placement chamber; 24. a sealing cover backing plate; 25. a slider; 26. fixing the rod; 27. a first spring; 28. an annular cavity; 29. a support pillar; 30. rotating the adjusting sleeve; 31. a second spring; 32. sliding the telescopic rod; 33. cushion blocks; 34. a first insulating layer; 35. A pressure relief valve; 36. a second insulating layer; 37. a first cooling chamber; 38. a flow control valve; 39. A second cooling chamber; 40. a nitrogen channel; 41. a limiting groove; 42. a first check valve; 43. a second one-way valve.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.

The first embodiment is as follows:

referring to fig. 1-7, the utility model provides a transportation and preservation device for peripheral blood DNA methylation detection, which comprises a box body 2, wherein a box cover 1 is hinged to the long edge of the top surface of the box body 2, a buffering heat-insulating body is arranged in the box cover 1 and the box body 2, a plurality of reagent bottles 7 are placed in the buffering heat-insulating body, a cooling mechanism for maintaining the low temperature of the reagent bottles 7 is arranged at the bottom of the inner side of the box body 2, and four corners of the bottom of the outer side of the box body 2 are respectively provided with a damping support 3;

buffering heat-preservation body includes that fixed connection is close to the first heat preservation main part 4 of 2 one sides of box at case lid 1, fixed connection is at the inboard second heat preservation main part 6 of box 2, first heat preservation main part 4 and the 6 top surface looks adaptations of second heat preservation main part, first heat preservation main part 4 is close to a plurality of stoppers 5 of 2 one side fixedly connected with of box, reagent bottle 7 sets up in second heat preservation main part 6, stopper 5 corresponds the setting with reagent bottle 7, and reagent bottle 7 is located cooling body's top, reagent bottle 7 is used for the sealed peripheral blood DNA methylation reagent of preserving. The box cover 1 and the box body 2 are mainly used for storing the reagent bottle 7, and the reagent bottle 7 has a good sealing effect; the cooling mechanism is mainly used for providing a continuous low-temperature storage environment for the reagent bottle 7, prolonging the storage period and maintaining the quality of the reagent; the main functions of the buffer heat-insulation body are that on one hand, the influence of external vibration on the reagent bottle 7 can be avoided, and on the other hand, the heat insulation can be carried out on the reagent bottle 7; the first thermal insulation body 4 and the stopper 5 help to fix the reagent bottle 7 in the second thermal insulation body 6.

Further optimize the scheme, reagent bottle 7 is including pegging graft test tube 12 in second heat preservation main part 6, test tube 12's the mouth of pipe is provided with sealed lid 13, the one end fixedly connected with sealing plug main part 14 at the bottom of the tube of test tube 12 is kept away from to sealed lid 13 inboard, test tube 12's the mouth of pipe is pegged graft between sealing plug main part 14 and sealed 13 lateral walls, be provided with rubber seal cover 15 between test tube 12 and the sealing plug main part 14, rubber seal cover 15 cup joints on sealing plug main part 14 lateral wall, sealing plug main part 14 center is provided with the exhaust portion that is used for discharging test tube 12 inside gas. The sealing cover 13 can seal the inside of the test tube 12 through the rubber sealing sleeve 15, so that the reagent in the test tube 12 is prevented from overflowing in the transportation process, external air can be prevented from entering the test tube 12, and the reagent is effectively prevented from being oxidized due to air contact; the main function of the exhaust part is to exhaust the redundant air in the test tube 12 when the reagent in the test tube 12 is sealed, thereby effectively avoiding the oxidation caused by the contact between the air and the reagent and influencing the long-term preservation of the reagent.

Further optimize the scheme, the exhaust portion is including seting up the constant voltage passageway 16 at sealing plug main part 14 center, the inboard grafting of the one end that constant voltage passageway 16 kept away from test tube 12 has the shutoff 17 that is used for sealing constant voltage passageway 16 of constant voltage passageway 16, the one end intercommunication that constant voltage passageway 16 is close to test tube 12 has the sealed piece activity chamber 18 of seting up at sealing plug main part 14 center, the exhaust hole 21 has been seted up to the one end that constant voltage passageway 16 was kept away from in sealed piece activity chamber 18, sealed piece activity chamber 18 inboard is provided with exhaust floating block 19, the one side and the exhaust hole 21 looks adaptation of exhaust floating block 19 near exhaust hole 21. When the sealing cover 13 is used for sealing the test tube 12, and the test tube 12 is vertically placed, namely the sealing cover 13 moves towards the tube bottom of the test tube 12, under the action of the rubber sealing sleeve 15, the reagent is effectively prevented from overflowing from the space between the sealing plug main body 14 and the wall of the test tube 12; when the sealing plug main body 14 increases the pressure in the test tube 12, the sealing plug main body 14 will continue to move, because the pressure in the test tube 12 increases, the air in the test tube 12 presses the air exhaust floating block 19, the air exhaust floating block 19 slides in the sealing block movable cavity 18 towards the direction of the constant pressure channel 16, meanwhile, the air in the test tube 12 overflows from the air exhaust hole of the air exhaust floating block 19 and is exhausted out of the test tube 12 through the constant pressure channel 16, when the air exhaust floating block 19 contacts the air inlet of the constant pressure channel 16, the air overflow is completed, at this time, under the internal pressure of the test tube 12, the air inlet of the constant pressure channel 16 is blocked by the air exhaust floating block 19, then the air outlet of the constant pressure channel 16 is blocked by the blocking block 17, and the sealing of the reagent is completed.

Further optimize the scheme, the edge of the air exhaust floating block 19 is connected with the inner side wall of the sealing block movable cavity 18 in a sliding mode, a plurality of air holes 20 are formed in the edge of the air exhaust floating block 19 at equal intervals in the circumferential direction, a circular truncated cone structure is arranged below the air exhaust floating block 19, and a protrusion is arranged on one side, far away from the air exhaust floating block 19, of the circular truncated cone structure and matched with the air exhaust hole 21. The blocking of the air inlet of the constant pressure passage 16 by the center position of the air exhaust floating block 19 can be accomplished only after the air in the test tube 12 is exhausted by disposing the air vents 20 at the edge of the air exhaust floating block 19.

Further optimization scheme, the recess with 4 looks adaptations of first heat preservation main part is seted up to 6 top surfaces of second heat preservation main part, the recess bottom surface is provided with places layer 601, place and offer a plurality of chambeies of accomodating that are used for placing a plurality of reagent bottles 7 on the layer 601, it is provided with the test tube and places chamber 23 to accomodate the intracavity, place and seted up spacing groove 41 on the layer 601, spacing groove 41 sets up places chamber 23 top and places chamber 23 intercommunication with the test tube at the test tube, spacing groove 41 and 5 looks adaptations of stopper, the test tube is placed and is provided with the fixed establishment that is used for fixed reagent bottle 7 in the chamber 23, stopper 5 and fixed establishment butt. After the reagent bottle 7 is placed in the test tube placing cavity 23, under the matching of the limiting block 5 and the limiting groove 41, the position stability of the reagent bottle 7 in the second heat preservation main body 6 is facilitated; the fixing mechanism can further limit the position of the reagent bottle 7 in the second heat preservation main body 6, and effectively prevents the reagent bottle 7 from moving due to bumping in the transportation process.

Further optimization scheme, the fixing mechanism comprises a sliding groove and a sealing cover plate 24 which are arranged at the bottom of the test tube placing cavity 23, the sliding groove is arranged at one end close to the tube bottom of the test tube 12, the sealing cover plate 24 is arranged at one end close to the sealing cover 13, a fixing rod 26 is fixedly connected in the sliding groove, one end of the fixing rod 26 far away from the tube bottom of the test tube 12 is fixedly connected at one end of the sealing cover plate 24 far away from the sealing cover 13, one end of the fixing rod 26 close to the tube bottom of the test tube 12 is fixedly connected at one side face of the sliding groove close to the tube bottom of the test tube 12, the central line of the fixing rod 26 is parallel to the central line of the test tube 12, a sliding block 25 is connected outside the fixing rod 26 in a sliding manner, the outer side of the sliding block 25 is connected in the sliding groove in a sliding manner, a first spring 27 is arranged between the sliding block 25 and the second heat preservation main body 6, the first spring 27 is sleeved outside the fixing rod 26, a test tube limiting shell 22 is hinged above the sliding block 25, the tube 12 is inserted inside the limiting shell 22, test tube 12 transversely sets up places the chamber 23 inboardly at the test tube, and sealed lid 13 one end of keeping away from test tube 12 places chamber 23 inside wall butts with the test tube, stopper 5 and the spacing 22 lateral walls butts of casing of test tube. Under the initial condition, the vertical upwards of the spacing casing 22 opening of test tube, insert the spacing casing 22 of test tube with the socle of test tube 12 in, then promote the spacing casing 22 of test tube to the direction of first spring 27, the spacing casing 22 of test tube drives the first spring 27 of slider 25 compression, after first spring 27 is compressed to the certain degree, rotate test tube 12, drive the spacing casing 22 of test tube and rotate to the direction of keeping away from first spring 27, make whole reagent bottle 7 level place, then move reagent bottle 7 along first spring 27 opposite direction, place chamber 23 inside wall contact with the test tube until the one end of the test tube 12 socle is kept away from to reagent bottle 7, at this moment, there is the resilience force after first spring 27 is extruded, and reagent bottle 7 places and test tube and forms the butt between the chamber 23 inside wall.

Further optimize the scheme, the torsional spring has been cup jointed in the articulated shaft outside of the spacing casing 22 of test tube and slider 25, places and is provided with a plurality of archs in the layer 601, protruding bottom and the 13 side butt of sealed lid, and the arch is located the one end top that sealed lid 13 kept away from test tube 12 socle. When the opening of the test tube limiting shell 22 is vertically upward, the torsion spring is in a free state, when the reagent bottle 7 is inserted into the test tube limiting shell 22 and moves to a horizontal position, the torsion spring is in a compressed state, resilience force is generated inside the torsion spring, and at the moment, the upper part of one end, far away from the tube bottom of the test tube 12, of the reagent bottle 7 cannot be bounced by the resilience force of the torsion spring under the limitation of the protrusion; the main effect of this arrangement is to subject the reagent bottle 7 to two forces, the first is from the axial force of the first spring 27, and the second is from the resilience force of the torsion spring, and the reagent bottle 7 can be more stably fixed in the second thermal insulation main body 6 by the two forces.

Further optimize the scheme, shock-absorbing support 3 is including seting up the annular cavity 28 in the box 2 bottom, annular cavity 28 center is provided with support column 29, support column 29 fixed connection is in the box 2 bottom, support column 29 bottom diameter is greater than support column 29 top diameter, support column 29 bottom outside spiro union has rotary adjusting sleeve 30, rotary adjusting sleeve 30 is located annular cavity 28, support column 29 bottom surface fixedly connected with slides telescopic link 32 one end, slide telescopic link 32 other end fixedly connected with cushion 33, be provided with second spring 31 between support column 29 bottom surface and the cushion 33, and second spring 31 sets up in the slide telescopic link 32 outside. In an initial state, the rotary adjusting sleeve 30 is far away from the cushion block 33, the box body 2 effectively absorbs the vibration transmitted to the box body 2 from the outside under the action of the second spring 31, and the second spring 31 absorbs the force from the vertical direction of the box body 2 under the vertical telescopic action of the sliding telescopic rod 32 of the box body 2; when the box body 2 is in a static state, namely the reagent bottle 7 is stored in an unmovable environment, the box body 2 is not vibrated in transportation any more, the rotary adjusting sleeve 30 is adjusted to the lowest end until the bottom end of the rotary adjusting sleeve 30 is contacted with the top surface of the cushion block 33, at the moment, the second spring 31 is in a free state, the rotary adjusting sleeve 30 can protect the second spring 31 and the sliding telescopic rod 32, the second spring 31 and the sliding telescopic rod 32 are used in a proper environment, and the damping effect of the box body 2 when needed is ensured.

According to a further optimized scheme, the cooling mechanism comprises a liquid nitrogen Dewar flask 8 arranged at the bottom of the inner side of the box body 2, a liquid inlet 9 is formed in one end, close to the side wall of the box body 2, of the liquid nitrogen Dewar flask 8, one end, far away from the liquid inlet 9, of the liquid nitrogen Dewar flask 8 is communicated with one end of a liquid outlet 10, the other end of the liquid outlet 10 is communicated with the bottom end of a second cooling cavity 39, the top end of the second cooling cavity 39 is communicated with a first cooling cavity 37 through a nitrogen channel 40, the first cooling cavity 37 is connected with a pressure release valve 35, and the first cooling cavity 37 is located below the reagent bottle 7; the top of the first cooling cavity 37 is provided with a low-temperature conducting layer 34, the bottom of the first cooling cavity 37 is provided with a heat insulating layer 36, the low-temperature conducting layer 34 is positioned between the reagent bottle 7 and the top of the first cooling cavity 37, and the heat insulating layer 36 is positioned between the bottom of the first cooling cavity 37 and the second cooling cavity 39. Liquid nitrogen can be supplemented into the liquid nitrogen Dewar flask 8 in time through the liquid inlet 9, a flow control valve 38 is connected between the liquid outlet 10 and the second cooling cavity 39, the flow control valve 38 can adjust the discharge amount of the liquid nitrogen, and the low temperature of the box body 2 can be accurately controlled; the low-temperature conductive layer 34 is used for transmitting the low temperature in the first cooling chamber 37 to the periphery of the reagent bottle 7 and maintaining the low-temperature storage environment of the reagent bottle 7; the low-temperature conducting layer 34 adopts a cold-conducting metal aluminum conducting layer, so that the weight is light, the cold-conducting property is good, and the cost is low; the heat-insulating layer 36 is a pearly-lustre sand layer and has good ultralow-temperature heat-insulating performance.

In a further optimized solution, a gap layer is formed between the first heat preservation body 4 and the placing layer 601, and the gap layer is communicated with the first cooling cavity 37 through the ventilation channel 11. The liquid nitrogen is gasified to absorb heat, the temperature of the surrounding environment is reduced, the low temperature is transmitted to the gap layer through the ventilation channel 11, and further a low-temperature storage environment is created for the reagent bottle 7.

In a further optimized scheme, the second cooling cavity 39, the first cooling cavity 37 and the gap layer are respectively connected with a temperature sensor, and the first cooling cavity 37 is internally connected with a pressure sensor. Temperature sensors are used to monitor the ambient temperature inside the second cooling chamber 39 and the first cooling chamber 37, and pressure sensors are used to monitor the nitrogen pressure inside the second cooling chamber 39 and the first cooling chamber 37.

Further optimize the scheme, relief valve 35, flow control valve 38, temperature sensor (not marked in the figure) and pressure sensor (not marked in the figure) electric connection have PLC controller (not shown in the figure), and PLC controller sets up in 2 inboard bottoms of box, PLC controller electric connection alarms.

In a further optimized scheme, a buckle (not shown in the figure) is arranged between the outer side wall of the box cover 1 and the outer side wall of the box body 2. The snap is used to lock the cover 1 to the housing 2.

The working process of the embodiment is as follows: after the reagent bottle 7 is fixed in the box body 2, the box cover 1 and the box body 2 are locked, then an opening instruction is transmitted to the flow control valve 38 through the PLC, then liquid nitrogen in the liquid nitrogen Dewar flask 8 flows into the second cooling cavity 39, the liquid nitrogen starts to volatilize after absorbing hot air in the cavity, the air temperature in the cavity starts to reduce, low temperature is transmitted to the first cooling cavity 37 through the nitrogen channel 40, and the temperature sensor and the pressure sensor monitor the low temperature and the low temperature pressure in the first cooling cavity 37; when the temperature is too low, the temperature sensor sends a low-temperature signal to the PLC, and the PLC transmits the converted signal to the flow control valve 38 to control the opening degree or opening time of the flow control valve 38; when the pressure is too high, the pressure sensor sends a high-pressure signal to the PLC, the PLC transmits the conversion signal to the pressure release valve 35, the opening of the pressure release valve 35 is controlled, and high-pressure gas in the cavity is released; the arrangement is such that the reagent bottle 7 can be kept in a low-temperature environment for a long time.

Can judge the liquid nitrogen surplus in the liquid nitrogen dewar bottle 8 through flow control valve 38 and PLC controller, and then supply the liquid nitrogen in to liquid nitrogen dewar bottle 8 through inlet 9, when the liquid nitrogen surplus in the liquid nitrogen dewar bottle 8 is not enough, the alarm can report to the police and indicate, and the alarm is prior art, and it is no longer detailed to describe here.

Example two

Referring to fig. 8, the damping mount of the present embodiment is different from the first embodiment only in that a first check valve 42 and a second check valve 43 are communicated with the rod-free cavity of the sliding telescopic rod 32, and the first check valve 42 and the second check valve 43 are respectively connected to the side walls of the sliding telescopic rod 32; the outlet size of the first check valve 41 is larger than the inlet size of the second check valve 43.

The working process of the embodiment is as follows: the box body 2 vibrates during transportation, when the box body 2 falls downwards, the second spring 31 is extruded, the sliding telescopic rod 32 is compressed downwards by the box body 2, at the moment, the pressure in the rodless cavity is increased and is greater than the opening pressure of the first one-way valve 42, air in the rodless cavity is discharged from the first one-way valve 42, when the second spring 31 absorbs the vibration, upward resilience force is generated, under the action of the resilience force, the box body 2 moves upwards, a negative pressure environment is formed in the rodless cavity, at the moment, the first one-way valve 42 is closed, the negative pressure is greater than the opening pressure of the second one-way valve 43, and because the air inlet of the second one-way valve 43 is smaller, the air in the rodless cavity slowly enters, the speed that slip telescopic link 32 resumes promptly is slow, and the speed that box 2 rises is slow, sets up like this and has just reached the range that reduces box 2 and reciprocate to reduce the vibration range of box 2, reached effectual shock attenuation effect.

In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, are merely for convenience of description of the present invention, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.

The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.

Claims (10)

1. A transportation and preservation device for peripheral blood DNA methylation detection is characterized in that: the reagent bottle box comprises a box body (2), wherein a box cover (1) is hinged to a long edge of the top surface of the box body (2), a buffering heat-insulating body is arranged in the box cover (1) and the box body (2), a plurality of reagent bottles (7) are placed in the buffering heat-insulating body, a cooling mechanism for maintaining the low temperature of the reagent bottles (7) is arranged at the bottom of the inner side of the box body (2), and four corners of the bottom of the outer side of the box body (2) are respectively provided with a damping support (3);

the buffer heat-preservation body comprises a box cover (1) fixedly connected with a first heat-preservation main body (4) and a second heat-preservation main body (6) which are close to one side of the box body (2), the first heat-preservation main body (4) is fixedly connected with the top surface of the second heat-preservation main body (6), the first heat-preservation main body (4) is close to a plurality of limit blocks (5) which are fixedly connected with one side of the box body (2), a reagent bottle (7) is arranged in the second heat-preservation main body (6), the limit blocks (5) are correspondingly arranged with the reagent bottle (7), the reagent bottle (7) is located above the cooling mechanism, and the reagent bottle (7) is used for sealing and preserving peripheral blood DNA methylation reagents.

2. The transportation and storage device for peripheral blood DNA methylation detection according to claim 1, wherein: reagent bottle (7) are including pegging graft test tube (12) in second heat preservation main part (6), the mouth of pipe of test tube (12) is provided with sealed lid (13), sealed lid (13) inboard is kept away from the one end fixedly connected with sealing plug main part (14) at the bottom of test tube (12) tube, the mouth of pipe of test tube (12) is pegged graft sealing plug main part (14) with between sealed lid (13) lateral wall, test tube (12) with be provided with rubber seal cover (15) between sealing plug main part (14), rubber seal cover (15) sealing plug cup joint on sealing plug main part (14) lateral wall, main part (14) center is provided with and is used for discharging test tube (12) inside gas's exhaust portion.

3. The transportation and storage device for peripheral blood DNA methylation detection according to claim 2, wherein: exhaust portion is including seting up at constant voltage passageway (16) at sealing plug main part (14) center, constant voltage passageway (16) are kept away from the inboard grafting of one end of test tube (12) has and is used for sealing shutoff (17) of constant voltage passageway (16), constant voltage passageway (16) are close to the one end intercommunication of test tube (12) has seted up sealing plug main part (14) center sealed piece activity chamber (18), sealed piece activity chamber (18) are kept away from exhaust hole (21) have been seted up to the one end of constant voltage passageway (16), sealed piece activity chamber (18) inboard is provided with exhaust floating block (19), exhaust floating block (19) are close to one side of exhaust hole (21) with exhaust hole (21) looks adaptation.

4. The transportation and storage device for peripheral blood DNA methylation detection according to claim 3, wherein: exhaust floating block (19) edge with sealed piece activity chamber (18) inside wall sliding connection, a plurality of bleeder vents (20) have been seted up to exhaust floating block (19) edge circumference equidistant, exhaust floating block (19) below sets up to the round platform structure, the round platform structure is kept away from one side of exhaust floating block (19) is provided with the arch, protruding with exhaust hole (21) looks adaptation.

5. The transportation and storage device for peripheral blood DNA methylation detection according to claim 4, wherein: the recess with first heat preservation main part (4) looks adaptation is seted up to second heat preservation main part (6) top surface, the grooved underside is provided with places layer (601), place and offer on layer (601) and be used for placing a plurality of the chamber is accomodate in a plurality of reagent bottles (7), it is provided with the test tube and places chamber (23) to accomodate the intracavity, place and seted up spacing groove (41) on layer (601), spacing groove (41) set up the test tube place chamber (23) top and with the test tube places chamber (23) intercommunication, spacing groove (41) with stopper (5) looks adaptation, the test tube is placed and is provided with in chamber (23) and is used for fixing the fixed establishment of reagent bottle (7), stopper (5) with the fixed establishment butt.

6. The transportation and storage device for peripheral blood DNA methylation detection according to claim 5, wherein: the fixing mechanism comprises a sliding groove and a sealing cover plate (24) which are arranged at the bottom of the test tube placing cavity (23), the sliding groove is arranged at a position close to one end of the tube bottom of the test tube (12), the sealing cover plate (24) is arranged at a position close to one end of the sealing cover (13), a fixing rod (26) is fixedly connected in the sliding groove, one end of the fixing rod (26) far away from the tube bottom of the test tube (12) is fixedly connected at one end of the sealing cover plate (24) far away from the sealing cover (13), one end of the fixing rod (26) close to the tube bottom of the test tube (12) is fixedly connected at one side face of the sliding groove close to the tube bottom of the test tube (12), the central line of the fixing rod (26) is parallel to the central line of the test tube (12), a sliding block (25) is connected at the outer side of the fixing rod (26) in a sliding manner, and the sliding block (25) is connected in the sliding groove, slider (25) with be provided with first spring (27) between second heat preservation main part (6), first spring (27) cup joint dead lever (26) outside, slider (25) top articulates there is spacing casing of test tube (22), test tube (12) socketed the spacing casing of test tube (22) inboard, test tube (12) transversely set up the test tube is placed chamber (23) inboard, sealed lid (13) are kept away from the one end of test tube (12) with the test tube is placed chamber (23) inside wall butt, stopper (5) with spacing casing of test tube (22) lateral wall butt.

7. The transportation and storage device for peripheral blood DNA methylation detection according to claim 6, wherein: spacing casing of test tube (22) with the articulated shaft outside of slider (25) has cup jointed the torsional spring, it is provided with a plurality of archs in layer (601) to place, protruding bottom with sealed lid (13) side butt, the arch is located sealed lid (13) are kept away from the one end top at test tube (12) socle.

8. The transportation and storage device for peripheral blood DNA methylation detection according to claim 1, wherein: the shock absorption support (3) comprises an annular cavity (28) arranged at the bottom of the box body (2), a supporting column (29) is arranged at the center of the annular cavity (28), the supporting column (29) is fixedly connected to the bottom of the box body (2), the diameter of the bottom end of the supporting column (29) is larger than that of the top end of the supporting column (29), the outer side of the bottom of the supporting column (29) is in threaded connection with a rotary adjusting sleeve (30), the rotary adjusting sleeve (30) is positioned in the annular cavity (28), one end of a sliding telescopic rod (32) is fixedly connected with the bottom surface of the supporting column (29), a cushion block (33) is fixedly connected at the other end of the sliding telescopic rod (32), a second spring (31) is arranged between the bottom surface of the supporting column (29) and the cushion block (33), and the second spring (31) is arranged outside the sliding telescopic rod (32).

9. The transportation and storage device for peripheral blood DNA methylation detection according to claim 5, wherein: the cooling mechanism comprises a liquid nitrogen Dewar flask (8) arranged at the bottom of the inner side of the box body (2), a liquid inlet (9) is formed in one end, close to the side wall of the box body (2), of the liquid nitrogen Dewar flask (8), one end, far away from the liquid inlet (9), of the liquid nitrogen Dewar flask (8) is communicated with one end of a liquid outlet (10), the other end of the liquid outlet (10) is communicated with the bottom end of a second cooling cavity (39), the top end of the second cooling cavity (39) is communicated with a first cooling cavity (37) through a nitrogen channel (40), the first cooling cavity (37) is connected with a pressure release valve (35), and the first cooling cavity (37) is located below the reagent bottle (7); the top of the first cooling cavity (37) is provided with a low-temperature conducting layer (34), the bottom of the first cooling cavity (37) is provided with a heat-insulating layer (36), the low-temperature conducting layer (34) is located between the reagent bottle (7) and the top of the first cooling cavity (37), and the heat-insulating layer (36) is located between the bottom of the first cooling cavity (37) and the second cooling cavity (39).

10. The transportation and storage device for peripheral blood DNA methylation detection according to claim 9, wherein: a gap layer is formed between the first heat preservation body (4) and the placing layer (601), and the gap layer is communicated with the first cooling cavity (37) through a ventilation channel (11).

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