CN215856152U - Embryo culture device - Google Patents
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- CN215856152U CN215856152U CN202023262787.9U CN202023262787U CN215856152U CN 215856152 U CN215856152 U CN 215856152U CN 202023262787 U CN202023262787 U CN 202023262787U CN 215856152 U CN215856152 U CN 215856152U
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Abstract
The utility model discloses an embryo culture device, which comprises: at least one embryo culture module capable of independently culturing, wherein the embryo culture module comprises a culture chamber and a culture assembly; the culture chamber comprises a top cover and a culture platform, wherein a cavity is formed below the top cover, the culture platform is slidably arranged in the cavity, and a sealed culture space for accommodating the culture assembly can be formed between the top cover and the culture platform; the culture assembly comprises a culture dish arranged on the culture platform, at least one embryo cover arranged in the culture dish, and a liquid inlet needle and a liquid discharge needle which are respectively arranged on two sides of the embryo cover. The utility model can realize the automatic liquid changing function in the embryo culture process and can avoid the influence of repeated unpacking and liquid changing operations on the embryo; the embryo culture chamber adopts a pull-type bin gate structure, and can be conveniently matched with an optical monitoring system to realize online monitoring; the utility model can effectively restrain the embryo from separating from the optical visual field during the liquid changing operation, can support the on-line automatic liquid changing and can realize the dynamic culture.
Description
Technical Field
The utility model relates to the field of embryo in-vitro culture, in particular to an embryo culture device.
Background
Birth defects have long been a significant problem in the reproductive health field. The total incidence rate of birth defects in China is about 5.6%, and the disease types mainly include congenital heart disease, cleft lip and palate and other structural deformities, phenylketonuria, severe spinal muscular atrophy and other hereditary diseases. Birth defects may occur at any stage of the pregnancy process, especially early stages. With the comprehensive opening of the two-birth policy in China in 2015, the prevention and treatment of birth defects become important scientific research directions in the field of reproductive biology. However, at present, there are many unbelievable puzzles on the physiological process of normal development of human embryos. Such as the dynamic changes of inactivation of the X chromosome, the process of early nervous system development, the mechanism of cardiac formation, etc., are important scientific problems that have not been clearly explained in the field of developmental biology. Therefore, the research on the normal pregnancy and the embryo development process of human beings not only helps people to further understand the origin of life and the embryo development, but also provides a theoretical basis for solving the major reproductive health problems such as birth defects and the like.
At present, in the field of assisted reproduction, the embryo in vitro culture technology is gradually mature, a common desktop or box type incubator can only realize basic culture functions, namely, basic environments such as temperature, humidity and CO2 mixed gas can be provided, real-time optical monitoring cannot be carried out, the embryo development details can be observed, the morphological change condition in the embryo development process can be recorded, or dynamic images possibly appearing in the later embryo development stage can be captured. In the long-term continuous development process of embryo, the demand to culture solution nutrient composition is dynamic change, and some metabolites also can slowly be released to embryo self, need in time remove, and present incubator all need take out the culture dish and carry out the liquid change operation, must lead to the fact potential influence to embryo development.
Therefore, a more reliable solution is now needed.
SUMMERY OF THE UTILITY MODEL
The present invention aims to solve the above-mentioned problems of the prior art and to provide an embryo culture device. The utility model can be conveniently integrated with an optical system to realize on-line monitoring, can realize the automatic liquid changing function, can avoid the influence of repeated box opening and liquid changing operations on embryos, and has the function of dynamic culture.
In order to achieve the purpose, the utility model adopts the technical scheme that: an embryo culture device comprising: at least one independently culturable embryo culture module comprising a culture chamber and a culture assembly;
the culture chamber comprises a top cover and a culture platform, wherein a cavity is formed below the top cover, the culture platform is slidably arranged in the cavity, a sealed culture space for accommodating the culture assembly is formed between the top cover and the culture platform, and the culture assembly is arranged on the culture platform;
the culture assembly comprises a culture dish arranged on the culture platform, at least one embryo cover arranged in the culture dish, and a liquid inlet needle and a liquid discharge needle which are respectively arranged at two sides of the embryo cover;
wherein the embryo cover is a cylindrical structure and is used for restraining the embryo in the space inside the embryo cover, and a plurality of through holes with the size capable of allowing the culture solution to pass and preventing the embryo from passing are arranged on the outer wall of the embryo cover.
Preferably, the embryo culturing device further comprises an optical monitoring system, the optical monitoring system comprises an illuminating module and an imaging module, the embryo culturing module is arranged between the illuminating module and the imaging module, a light-transmitting observation channel is arranged on the embryo culturing module, the embryo cover is arranged in the observation visual field range of the observation channel, and light emitted by the illuminating module can reach the imaging module after illuminating the embryo cover through the observation channel, so that the embryo cultured in the embryo cover can be observed by the optical monitoring system through the observation channel.
Preferably, the cross section of the top cover is U-shaped, the top cover comprises a flat plate and side edges connected with the lower parts of two sides of the flat plate, and the bottom surface of the flat plate protrudes downwards to form a top cover inclined plane;
the upper end edge of the culture platform forms a platform inclined plane matched with the top cover inclined plane, and the inclined angle of the top cover inclined plane is the same as that of the platform inclined plane, so that sealing matching can be realized through mutual contact of the top cover inclined plane and the platform inclined plane.
Preferably, an accommodating groove is formed in the middle of the top cover inclined surface upwards, a sealing groove is formed in the top cover inclined surface and surrounds the accommodating groove, and a sealing strip is arranged in the sealing groove;
after the culture platform is pushed into the top cover, the sealing strip is in contact with the platform inclined plane, so that sealing is realized.
Preferably, the outer end of the culture platform is connected with a latch hook through a hinge, a locking block is arranged on a flat plate at an inlet of the top cover, a locking groove is formed in the locking block, and a locking rod matched with the latch hook is connected in the locking groove;
after the inner end of the culture platform is pushed into the top cover in place, the lock hook can extend into the locking groove and hook and pull the lock rod to realize locking;
the outside of latch hook has arc portion, the bottom surface in locking groove be provided with arc portion complex arc guide inclined plane.
Preferably, the upper surface of the flat plate and the bottom surface of the outer side of the culture platform are both attached with heating elements and temperature sensors;
the bottom surface inboard of culture platform is offered and is used for setting up the mounting groove of culture dish and be used for with the spiral groove that the feed liquor pipeline ring winding that the feed liquor needle is connected was arranged.
Preferably, the culture assembly further comprises a fixed seat and a T-shaped liquid inlet distribution block arranged on the fixed seat, a liquid inlet channel and a plurality of liquid outlet channels communicated with the liquid inlet channel are arranged in the liquid inlet distribution block, and the tail ends of the liquid outlet channels are connected with the liquid inlet needles;
the liquid inlet needles are in one-to-one correspondence with the embryo covers, the outlet ends of the liquid inlet needles are horizontally arranged and just face the embryo covers, and the inlet ends of the liquid discharge needles are close to the inner wall of the bottom of the culture dish.
Preferably, a through groove is formed in the middle of the fixed seat in a penetrating manner along the vertical direction, and an installation groove communicated with the through groove is formed in the surface of the first side of the fixed seat; a supporting strip fixedly connected with the inner wall of the through groove is arranged in the through groove, the supporting strip divides the through groove into a sample adding groove and an observation groove, and the observation groove is positioned right above the embryo covers;
t type feed liquor distribution piece including advance kind of portion and with the perpendicular distribution portion of connecting of kind portion, connect in the distribution portion a plurality of liquid needles of advancing, advance kind of portion cooperation and set up in the mounting groove, the distribution portion overlap joint is in on the support bar, a plurality of feed liquor needles pass the application of sample groove stretches into in the culture dish.
Preferably, two circular holes are vertically perforated on two sides of the mounting groove, a bushing is fixedly arranged in each circular hole, a positioning pin is slidably inserted in each bushing, and the bottom of each positioning pin is connected with the culture platform;
the bottom surface of the first side of the fixed seat is provided with an arc-shaped sinking groove, and the two round holes are respectively communicated with the two sides of the sinking groove;
the spring wire is arranged in the sinking groove, the spring wire is restrained in the sinking groove through a plurality of pressing blocks which are arranged below the sinking groove and connected with the fixing seat, and the distance from the position, closest to the center of the round hole, on the spring wire to the center of the round hole is smaller than the radius of the round hole, so that after the positioning pin is inserted into the bushing, the spring wire can be in contact with the positioning pin and can extrude the positioning pin.
Preferably, the upper surface of the flat plate is provided with a first glass window, the mounting groove is internally provided with a second glass window, and the first glass window, the observation groove and the second glass window are opposite in position in the vertical direction, so that the observation channel capable of penetrating through illumination light is formed.
The utility model has the beneficial effects that:
the embryo culture module can realize the automatic liquid changing function in the embryo culture process, and can avoid the influence of repeated box opening and liquid changing operations on embryos;
the embryo culture chamber adopts a pull-type bin gate structure, and can be conveniently matched with an optical monitoring system to realize online monitoring;
the culture assembly can effectively restrain the embryo from being separated from the optical visual field during the liquid changing operation, can support the on-line automatic liquid changing and can realize dynamic culture.
Drawings
FIG. 1 is a schematic diagram of an embryo culture module in an embodiment of the present invention;
FIG. 2 is a schematic view of the structure of a culture assembly in an embodiment of the present invention;
FIG. 3 is a schematic diagram of one form of embryo shield in an embodiment of the present invention;
FIG. 4 is a schematic diagram of another form of embryo shield in an embodiment of the present invention;
FIG. 5 is a schematic diagram of an embryo culture device in an embodiment of the present invention;
FIG. 6 is a schematic diagram of another perspective of an embryo culture device in an embodiment of the present invention;
FIG. 7 is a schematic bottom view of an embryo culture module in an embodiment of the present invention;
FIG. 8 is a schematic structural view of a top cover in an embodiment of the present invention;
FIG. 9 is a schematic structural view of a culture platform in an embodiment of the present invention;
FIG. 10 is an enlarged partial view of FIG. 1 at A in accordance with the present invention;
FIG. 11 is a schematic cross-sectional view of an embryo culture module in an embodiment of the present invention;
FIG. 12 is an exploded view of some of the components of an embryo culture module in an embodiment of the utility model;
FIG. 13 is a schematic structural view of a culture assembly in an embodiment of the present invention;
fig. 14 is a schematic view of a T-shaped liquid inlet distribution block in cooperation with a fixed seat in an embodiment of the present invention;
FIG. 15 is a schematic structural view of the bottom of the culture assembly in the example of the present invention (with the petri dish removed);
fig. 16 is a schematic structural view of the bottom of the fixing base in the embodiment of the utility model.
Description of reference numerals:
1-embryo culture module; 2-culture room; 3-a culture assembly; 4-observation channel; 5-optical monitoring system; 50-screw motor driving mechanism;
20-a top cover; 21-a cavity; 22-culture platform; 23-a heating element; 24-insulating layer; 25-linear guide rail;
200-plate; 201-side; 202-top cover ramp; 203-accommodating grooves; 204-a sealing groove; 205-sealing strip; 206-a locking block; 207-locking groove; 208-locking lever; 209-arc guide slope; 2000-first glass window;
220-platform slope; 221-a latch hook; 222-an arc; 223-mounting the groove; 224-a helical groove; 225 — a second glass window;
30-culture dish; 31-embryo shield; 32-T type feed liquor distribution block; 33-a liquid inlet needle; 34-a drainage needle; 235-a fixed seat; 236-through groove; 237-mounting groove; 238-supporting bar; 2310-through holes; 2311-flange structure; 2320-sample introduction part; 2321 — a distributing section; 2350-round hole; 2351-a liner; 2352-locating pin; 2353-sink tank; 2354-spring wire; 2355-mounting holes; 2356-briquetting; 2360 sample addition slot; 2361 observation tank.
Detailed Description
The present invention is further described in detail below with reference to examples so that those skilled in the art can practice the utility model with reference to the description.
It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
Example 1
Referring to fig. 1 to 12, an embryo culture apparatus of the present embodiment includes: at least one embryo culture module 1 capable of independently culturing, wherein the embryo culture module 1 comprises a culture chamber 2 and a culture assembly 3;
the culture chamber 2 comprises a top cover 20 and a culture platform 22, wherein a cavity 21 is formed below the top cover 20, the culture platform 22 is slidably arranged in the cavity 21, a sealed culture space for accommodating the culture component 3 can be formed between the top cover 20 and the culture platform 22, and the culture component 3 is arranged on the culture platform 22;
the culture assembly 3 comprises a culture dish 30 arranged on the culture platform 22, at least one embryo cover 31 arranged in the culture dish 30, and a liquid inlet needle 33 and a liquid discharge needle 34 which are respectively arranged at two sides of the embryo cover 31;
wherein the embryo enclosure 31 is a cylindrical structure for confining an embryo in the space inside thereof, one embryo enclosure 31 accommodates one embryo therein. The outer wall of the embryo shield 31 is provided with a number of through holes 2310 of a size that allows the passage of culture medium but prevents the passage of embryos. The holes 2310 are arranged to allow liquid to freely pass through the embryo cover 31 and keep the embryo confined therein, so that the embryo can be prevented from flowing with the liquid and exceeding the observation visual field when liquid exchange is carried out. The embryo is cultured in the culture dish 30 and is restrained by the embryo cover 31 to keep the position fixed, thereby being convenient for monitoring and observation. The structure of the through hole 2310 on the outer wall of the embryo shield 31 can be obtained by integrally forming a plurality of grid holes (as shown in fig. 3) on the outer wall of the embryo shield 31, or integrally forming a hollowed-out frame (as shown in fig. 4) and then winding a layer of microporous net on the hollowed-out frame. In one embodiment, the size of the through hole 2310 of the embryo cover 31 is about 80-100 μm, the early embryo is approximately 150-200 μm spheroid, the embryo cannot pass through the grid hole, and the culture solution inside and outside the embryo cover 31 can be freely exchanged. In a preferred embodiment, the inside diameter of the embryo shield 31 is about 5mm and the height is about 6mm, and the bottom of the embryo shield 31 has a flange 2311 for easy attachment to the bottom surface of the culture dish 30.
In a preferred embodiment, the embryo culture apparatus further comprises an optical monitoring system 5, which may be a conventional optical monitoring system 5 in the form of an inverted microscope for imaging embryos, comprising an illumination module and an imaging module, the embryo culture module 1 being positioned between the illumination module and the imaging module, the illumination module providing illumination, and the imaging module being used for monitoring and imaging embryos. The embryo culture mold is provided with a light-transmitting observation channel 4, the embryo cover 31 is positioned in the observation visual field range of the observation channel 4, and light emitted by the illumination module can reach the imaging module after illuminating the embryo cover 31 through the observation channel 4, so that the optical monitoring system 5 can observe embryos cultured in the embryo cover 31 through the observation channel 4. Referring to FIG. 6, in a preferred embodiment, the embryo culture apparatus further comprises a driving mechanism for realizing the linear movement of the optical monitoring system 5, the driving mechanism can adopt a conventional screw motor driving mechanism 50, and the optical monitoring system 5 is driven to move linearly, so that a plurality of embryo culture apparatuses arranged in a single row in a linear manner can be respectively monitored.
In this embodiment, the embryo culture chamber 2 is a pull-type door structure, the top cover 20 and the culture platform 22 are slidably matched through the linear guide rail 25, the slide block and the guide rail of the linear guide rail 25 are respectively installed at the proper positions of the side edges 201 of the top cover 20 and the culture platform 22, and the top cover 20 and the culture platform 22 can slide relatively in the horizontal direction under the constraint of the linear guide rail 25, so that the push-pull operation is realized. The drawing moving direction of the culture platform 22 is vertical to the moving direction of the optical monitoring system 5, and the embryo culture chamber 2 is of a drawing structure, so that observation of a plurality of embryo culture modules 1 can be realized through one optical monitoring system 5, and no space interference phenomenon exists.
Referring to fig. 8, in a preferred embodiment, the top cover 20 has a U-shaped cross section, and includes a flat plate 200 and side edges 201 connected to the lower sides of the flat plate 200, wherein the bottom surface of the flat plate 200 protrudes downward to form a top cover inclined surface 202;
the upper edge of the culture platform 22 forms a platform inclined plane 220 matched with the top cover inclined plane 202, and the inclined angles of the top cover inclined plane 202 and the platform inclined plane 220 are the same, so that the top cover inclined plane 202 and the platform inclined plane 220 can be in sealing fit through mutual contact.
The middle part of the top cover inclined surface 202 is upwards provided with an accommodating groove 203, a sealing groove 204 is arranged on the top cover inclined surface 202 and surrounds the accommodating groove 203, and a sealing strip 205 is arranged in the sealing groove 204; after the growth platform 22 is pushed into the lid 20, the sealing strip 205 contacts the platform ramp 220 to effect a seal.
In a further preferred embodiment, the outer end of the culture platform 22 is connected with a latch hook 221 through a hinge, a locking block 206 is arranged on the flat plate 200 at the inlet of the top cover 20, a locking groove 207 is formed on the locking block 206, and a lock rod 208 used for being matched with the latch hook 221 is connected in the locking groove 207; after the inner end of the culture platform 22 is pushed into the top cover 20 to a proper position, the locking hook 221 can extend into the locking groove 207 and hook the locking rod 208 to realize locking.
Still further, the outer side of the locking hook 221 has an arc portion 222, and the bottom surface of the locking groove 207 is provided with an arc guide slope 209 which is matched with the arc portion 222. When the culture platform 22 is pushed into the top cover 20 and the lock hook 221 extends into the locking groove 207, the arc part 222 of the lock hook 221 is in fit contact with the arc guide inclined surface 209, so that the lock hook 221 can automatically rotate anticlockwise to hook the lock rod 208 from bottom to top, and because the top cover inclined surface 202 is in contact fit with the inclined surface of the platform inclined surface 220, the top cover 20 has a movement tendency of being separated from the culture platform 22, so that the lock hook 221 forms a pretightening force on the lock rod 208, the top cover 20 and the culture platform 22 are locked, and the sealing between the top cover 20 and the culture platform 22 is ensured. When the culture platform 22 needs to be pulled out, the outer end of the locking hook 221 is pulled upwards manually, so that the locking hook 221 rotates anticlockwise to be separated from the locking lever 208, and the culture platform 22 can be pulled out from the top cover 20 easily.
In a preferred embodiment, the upper surface of the plate 200 and the outer bottom surface of the culture platform 22 are covered with a heating element 23 and a temperature sensor; the heating element 23 is used for heating the culture chamber 2, and the temperature sensor detects the temperature so as to cooperate with the heating element 23 to realize the temperature control of the culture chamber 2. In a further preferred embodiment, the heating element 23 is also covered with an insulating layer 24.
Further, the inner side of the bottom surface of the culture platform 22 is provided with a mounting groove 223 for arranging the culture dish 30 and a spiral groove 224 for annularly winding a liquid inlet pipeline (not shown in the figure) connected with the liquid inlet needle 33. Because the bottom of the culture platform 22 is attached with the heating element 23, the liquid inlet pipeline is wound and embedded in the spiral groove 224, and the culture liquid in the liquid inlet pipeline can be rapidly heated.
The mounting groove 223 is used for arranging the culture dish 30, the culture dish 30 can be a general culture dish 30, such as a 35mm dish, a 60mm dish, or a special stationary culture dish 30, and the mounting groove 223 is configured to be matched with the culture dish 30 in shape and size.
Example 2
Referring to fig. 13-16, as a further improvement on embodiment 1, in this embodiment, the cultivation assembly 3 further includes a fixing base 235 and a T-shaped liquid inlet distribution block 32 disposed on the fixing base 235, a liquid inlet channel and a plurality of liquid outlet channels (i.e. one-to-many channels, in this embodiment, 1-to-4 channels, not shown in the figures) are disposed in the liquid inlet distribution block, and the end of the liquid outlet channel is connected to a liquid inlet needle 33;
the liquid inlet pins 33 correspond to the embryo covers 31 one by one, that is, the first side of each embryo cover 31 has one sample adding pin, in this embodiment, the liquid inlet pins include 4 embryo covers 31 arranged in a row, and each embryo cover 31 is configured with one sample adding pin. The outlet end of the liquid inlet needle 33 is horizontally arranged and is opposite to the embryo shield 31, and the inlet end of the liquid outlet needle 34 is close to the inner wall of the bottom of the culture dish 30. During liquid changing operation, the culture solution can be divided into multiple paths after entering the T-shaped liquid inlet distribution block 32, the multiple paths are injected into the culture dish 30 through the sample adding needle, and then the waste liquid needle is used for removing the redundant culture solution, so that liquid changing is realized; during dynamic culture, the culture solution enters the T-shaped liquid inlet distribution block 32 and then is divided into multiple paths, the liquid inlet needle 33 performs reciprocating perfusion and suck-back actions on the embryo cover 31, dynamic liquid flow is presented in the embryo cover 31, and a fluid shearing effect is generated on embryos, so that the dynamic culture function can be realized.
Wherein, the middle part of the fixed seat 235 is provided with a through groove 236 in a penetrating manner along the vertical direction, and the surface of the first side of the fixed seat 235 is provided with a mounting groove 237 communicated with the through groove 236; a supporting bar 238 fixedly connected with the inner wall of the through groove 236 is arranged in the through groove 236, the supporting bar 238 divides the through groove 236 into a sample adding groove 2360 and an observation groove 2361, and the observation groove 2361 is positioned right above the plurality of embryo covers 31; so that embryos in the embryo shields 31 can be observed through the observation grooves 2361.
The T-shaped liquid inlet distribution block 32 comprises a sample injection part 2320 and a distribution part 2321 vertically connected with the sample injection part 2320, the distribution part 2321 is connected with a plurality of liquid inlet needles 33, the sample injection part 2320 is arranged in the mounting groove 237 in a matching manner, the distribution part 2321 is lapped on the supporting bar 238, and the liquid inlet needles 33 penetrate through the sample injection groove 2360 and extend into the culture dish 30, so that the culture solution is conveyed.
Wherein, two sides of the mounting groove 237 are vertically provided with two round holes 2350 in a through manner, a bush 2351 is fixedly arranged in the round hole 2350, a positioning pin 2352 is slidably inserted in the bush 2351, and the bottom of the positioning pin 2352 is connected with the culture platform 22;
an arc-shaped sinking groove 2353 is formed in the bottom surface of the first side of the fixed seat 235, and the two round holes 2350 are respectively communicated with two sides of the sinking groove 2353;
the spring wire 2354 is arranged in the sinking groove 2353, the spring wire 2354 is restrained in the sinking groove 2353 through a plurality of pressing blocks 2356 which are arranged below the sinking groove 2353 and connected with the fixing seat 235, the spring wire 2354 is in a pre-tightening state in the sinking groove 2353, and the distance from the position, closest to the center of the round hole 2350, on the spring wire 2354 to the center of the round hole 2350 is smaller than the radius of the round hole 2350, so that after the positioning pin 2352 is inserted into the bushing 2351, the spring wire 2354 can be in contact with the positioning pin 2352 and can extrude the positioning pin 2352.
Cultivate subassembly 3 integral erection on cultivateing room 2, the lower extreme of locating pin 2352 is connected on cultivateing room 2, fixing base 235 is established through bush 2351 cover and is installed on locating pin 2352, fixing base 235 can slide from top to bottom on locating pin 2352, because spring 2354 can extrude the disc outside locating pin 2352, the clearance between bush 2351 and locating pin 2352 has been eliminated, guarantee certain contact rigidity, thereby produce certain extrusion frictional force and make fixing base 235 can stop and can not freely fall in any position of locating pin 2352 up-and-down sliding stroke, the installation is more reliable.
In a preferred embodiment, the fixing base 235 further defines a mounting hole 2355 for installing the drainage needle 34.
In a preferred embodiment, a first glass window 2000 is disposed on the upper surface of the plate 200, a second glass window 225 is disposed in the mounting groove 223, and the first glass window 2000, the observation groove 2361 and the second glass window 225 are vertically opposite to each other, so as to form an observation channel 4 through which illumination light can pass.
While embodiments of the utility model have been disclosed above, it is not limited to the applications listed in the description and the embodiments, which are fully applicable in all kinds of fields of application of the utility model, and further modifications may readily be effected by those skilled in the art, so that the utility model is not limited to the specific details without departing from the general concept defined by the claims and the scope of equivalents.
Claims (10)
1. An embryo culture device, comprising: at least one independently culturable embryo culture module comprising a culture chamber and a culture assembly;
the culture chamber comprises a top cover and a culture platform, wherein a cavity is formed below the top cover, the culture platform is slidably arranged in the cavity, a sealed culture space for accommodating the culture assembly is formed between the top cover and the culture platform, and the culture assembly is arranged on the culture platform;
the culture assembly comprises a culture dish arranged on the culture platform, at least one embryo cover arranged in the culture dish, and a liquid inlet needle and a liquid discharge needle which are respectively arranged at two sides of the embryo cover;
wherein the embryo cover is a cylindrical structure and is used for restraining the embryo in the space inside the embryo cover, and a plurality of through holes with the size capable of allowing the culture solution to pass and preventing the embryo from passing are arranged on the outer wall of the embryo cover.
2. The embryo culture device according to claim 1, further comprising an optical monitoring system, wherein the optical monitoring system comprises an illumination module and an imaging module, the embryo culture module is located between the illumination module and the imaging module, the embryo culture module is provided with a light-transmitting observation channel, the embryo cover is located within the observation visual field range of the observation channel, and light emitted from the illumination module illuminates the embryo cover through the observation channel and then reaches the imaging module, so that the embryo cultured in the embryo cover can be observed by the optical monitoring system through the observation channel.
3. The embryo culture device according to claim 2, wherein the top cover has a U-shaped cross section, and comprises a flat plate and side edges connected with the lower parts of two sides of the flat plate, and the bottom surface of the flat plate protrudes downwards to form a top cover inclined surface;
the upper end edge of the culture platform forms a platform inclined plane matched with the top cover inclined plane, and the inclined angle of the top cover inclined plane is the same as that of the platform inclined plane, so that sealing matching can be realized through mutual contact of the top cover inclined plane and the platform inclined plane.
4. The embryo culture device according to claim 3, wherein a receiving groove is formed upward in the middle of the top cover inclined surface, a sealing groove is formed on the top cover inclined surface and surrounds the receiving groove, and a sealing strip is arranged in the sealing groove;
after the culture platform is pushed into the top cover, the sealing strip is in contact with the platform inclined plane, so that sealing is realized.
5. The embryo culture device according to claim 4, wherein the outer end of the culture platform is connected with a latch hook through a hinge, a flat plate at the inlet of the top cover is provided with a locking block, the locking block is provided with a locking groove, and a locking rod matched with the latch hook is connected in the locking groove;
after the inner end of the culture platform is pushed into the top cover in place, the lock hook can extend into the locking groove and hook and pull the lock rod to realize locking;
the outside of latch hook has arc portion, the bottom surface in locking groove be provided with arc portion complex arc guide inclined plane.
6. The embryo culture device according to claim 3, wherein the heating element and the temperature sensor are attached to the upper surface of the flat plate and the bottom surface of the outer side of the culture platform;
the bottom surface inboard of culture platform is offered and is used for setting up the mounting groove of culture dish and be used for with the spiral groove that the feed liquor pipeline ring winding that the feed liquor needle is connected was arranged.
7. The embryo culturing device according to claim 1, wherein the culturing assembly further comprises a fixing seat and a T-shaped liquid inlet distribution block arranged on the fixing seat, a liquid inlet channel and a plurality of liquid outlet channels communicated with the liquid inlet channel are arranged in the liquid inlet distribution block, and the tail ends of the liquid outlet channels are connected with the liquid inlet needles;
the liquid inlet needles are in one-to-one correspondence with the embryo covers, the outlet ends of the liquid inlet needles are horizontally arranged and just face the embryo covers, and the inlet ends of the liquid discharge needles are close to the inner wall of the bottom of the culture dish.
8. The embryo culture device according to claim 7, wherein a through groove is formed in the middle of the fixing seat in a penetrating manner along the vertical direction, and a mounting groove communicated with the through groove is formed in the surface of the first side of the fixing seat; a supporting strip fixedly connected with the inner wall of the through groove is arranged in the through groove, the supporting strip divides the through groove into a sample adding groove and an observation groove, and the observation groove is positioned right above the embryo covers;
t type feed liquor distribution piece including advance kind of portion and with the perpendicular distribution portion of connecting of kind portion, connect a plurality of liquid needles of advancing on the distribution portion, advance kind of portion cooperation and set up in the mounting groove, the distribution portion overlap joint is in on the support bar, a plurality of liquid needles pass the application of sample groove stretches into in the culture dish.
9. The embryo culture device according to claim 8, wherein two circular holes are vertically perforated on two sides of the mounting groove, a bushing is fixedly arranged in each circular hole, a positioning pin is slidably inserted in each bushing, and the bottom of each positioning pin is connected with the culture platform;
the bottom surface of the first side of the fixed seat is provided with an arc-shaped sinking groove, and the two round holes are respectively communicated with the two sides of the sinking groove;
the spring wire is arranged in the sinking groove, the spring wire is restrained in the sinking groove through a plurality of pressing blocks which are arranged below the sinking groove and connected with the fixing seat, and the distance from the position, closest to the center of the round hole, on the spring wire to the center of the round hole is smaller than the radius of the round hole, so that after the positioning pin is inserted into the bushing, the spring wire can be in contact with the positioning pin and can extrude the positioning pin.
10. The embryo culture device according to claim 6, wherein the upper surface of the plate is provided with a first glass window, the mounting groove is provided with a second glass window, and the first glass window, the observation groove and the second glass window are vertically opposite to each other, so as to form the observation channel which can be penetrated by illumination light.
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| CN202023262787.9U CN215856152U (en) | 2020-12-29 | 2020-12-29 | Embryo culture device |
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| CN202023262787.9U CN215856152U (en) | 2020-12-29 | 2020-12-29 | Embryo culture device |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112625907A (en) * | 2020-12-29 | 2021-04-09 | 中国科学院苏州生物医学工程技术研究所 | Embryo long-term culture system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112625907A (en) * | 2020-12-29 | 2021-04-09 | 中国科学院苏州生物医学工程技术研究所 | Embryo long-term culture system |
| CN112625907B (en) * | 2020-12-29 | 2024-06-25 | 中国科学院苏州生物医学工程技术研究所 | Embryo long-time culture system |
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