CN215757393U - Magnetic bead separation device for separating adeno-associated virus particles - Google Patents
Magnetic bead separation device for separating adeno-associated virus particles Download PDFInfo
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- CN215757393U CN215757393U CN202122174140.9U CN202122174140U CN215757393U CN 215757393 U CN215757393 U CN 215757393U CN 202122174140 U CN202122174140 U CN 202122174140U CN 215757393 U CN215757393 U CN 215757393U
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Abstract
The utility model discloses a magnetic bead separation device for separating adeno-associated virus particles, which comprises a magnetic bead separation box, wherein a magnetic bead separation cavity and a separated particle collection cavity are arranged in the magnetic bead separation box, a magnet supporting plate is arranged at the bottom end of an inner cavity of the magnetic bead separation cavity, a scraper is arranged at one end of the top of the magnet supporting plate, and one end of the scraper is connected with a telescopic end of a second electric push rod; and a feeding head is arranged on one side of the top end of the magnetic bead separation cavity. The utility model discloses a bottom of top cap is equipped with the tongue, and the bottom both sides of top cap all are equipped with first electric push rod, can seal the top cap on the feed inlet automatically through it to it is sealed by the tongue embedding, sealed effect is higher, and the middle part of top cap is equipped with the stirring frame by motor control, be equipped with the stirring leaf in the both sides of stirring frame, can stir the granule that needs the separation through it, make it under centrifugal action, the separation is accomplished to the cooperation magnet layer board, this kind of mode effectively improves the efficiency of separation.
Description
Technical Field
The utility model relates to the field of magnetic bead separation devices, in particular to a magnetic bead separation device for separating adeno-associated virus particles.
Background
The cells are specifically labeled with superparamagnetism, after the cells are magnetically labeled, the cells are put through a sorting column placed in a strong and stable magnetic field, a matrix in the sorting column causes a high gradient magnetic field, and the magnetically labeled cells are retained in the column while the unlabeled cells are discharged. When the sorting column is removed from the magnetic field, the magnetically labeled cells in the retention column can be eluted, so that both labeled and unlabeled cell fractions are fully available.
The magnetic bead separator at present stage is reinforced and gets the material inconvenient inadequately, and can't carry out once only to accomplish the separation operation in a large number, needs frequently to add the raw materials, leads to efficiency greatly reduced, and automatic effect is poor, after the magnetic bead is screened, under magnetic force, makes its separation take out comparatively troublesome, and adsorbs easily on the bottom plate, influences subsequent use.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a magnetic bead separation device for separating adeno-associated virus particles, and aims to solve the problems that the magnetic bead separation device provided in the background art is inconvenient to feed and take materials, cannot complete separation operation in a large amount at one time, needs to frequently feed raw materials, greatly reduces efficiency, has poor automatic effect, is troublesome to separate and take out under the action of magnetic force after magnetic beads are screened, and is easy to adsorb on a bottom plate, so that subsequent use is influenced.
In order to achieve the purpose, the utility model provides the following technical scheme: a magnetic bead separation device for separating adeno-associated virus particles comprises a magnetic bead separation box, wherein a magnetic bead separation cavity and a separated particle collection cavity are arranged in the magnetic bead separation box, a magnet supporting plate is arranged at the bottom end of an inner cavity of the magnetic bead separation cavity, a scraper is arranged at one end of the top of the magnet supporting plate, and one end of the scraper is connected with a telescopic end of a second electric push rod;
the magnetic bead separation chamber is characterized in that a feeding head is arranged on one side of the top end of the magnetic bead separation chamber, a top cover is arranged right above the feeding head, a convex groove is arranged in the middle of the bottom end of the top cover and embedded into the feeding head, and the two sides of the bottom of the top cover are embedded and fixed with the telescopic end of the first electric push rod.
In a further embodiment, the middle part of top cap is equipped with the motor, and the one end of motor is equipped with the stirring frame, the both sides of stirring frame all are equipped with the stirring leaf.
In a further embodiment, a material conveying groove is formed between the magnetic bead separation cavity and the separated particle collection cavity, a material discharging head is arranged at a position below one end of the separated particle collection cavity, a material taking opening is formed in the top of the separated particle collection cavity, and a sealing cover is installed on the material taking opening.
In a further embodiment, the cylinder body of the first electric push rod is embedded in the magnetic bead separation box, embedding grooves are formed in two sides of the bottom of the top cover, and the front end of the cylinder body can be embedded in the embedding grooves.
In a further embodiment, a material guide cavity with an electromagnetic valve is arranged on one side of the magnetic bead separation cavity, and a magnetic bead discharge port is arranged on the front surface of the material guide cavity.
In a further embodiment, the bottom end of the magnetic bead separation box is provided with a bracket, and the bottom of the bracket is provided with a stabilizing base.
Compared with the prior art, the utility model has the beneficial effects that:
1. the utility model discloses a bottom of top cap is equipped with the tongue, and the bottom both sides of top cap all are equipped with first electric push rod, can seal the top cap on the feed inlet automatically through it to it is sealed by the tongue embedding, sealed effect is higher, and the middle part of top cap is equipped with the stirring frame by motor control, be equipped with the stirring leaf in the both sides of stirring frame, can stir the granule that needs the separation through it, make it under centrifugal action, the separation is accomplished to the cooperation magnet layer board, this kind of mode effectively improves the efficiency of separation.
2. The utility model discloses a top of magnet layer board is equipped with the scraper blade by second electric push rod control, makes it scrape the magnetic bead of adsorptive separation on the magnet layer board, makes its convenient subsequent use, and conveniently gets the material, can avoid causing the pollution to the magnet layer board.
Drawings
FIG. 1 is a schematic structural diagram of a magnetic bead separation device for separating adeno-associated virus particles according to the present invention;
FIG. 2 is a schematic view of a magnetic bead separation chamber according to the present invention;
FIG. 3 is a top view of a magnetic bead separation chamber according to the present invention;
fig. 4 is a front view of the magnet pallet of the present invention.
In the figure: 1. a top cover; 2. a convex groove; 3. a motor; 4. a stirring frame; 5. stirring blades; 6. a first electric push rod; 7. a feed head; 8. a second electric push rod; 9. a bracket; 10. a stabilizing base; 11. a magnetic bead discharge port; 12. a sealing cover; 13. a magnetic bead separation box; 14. a discharge head; 15. a material taking port; 16. a squeegee; 17. a magnet supporting plate; 18. a magnetic bead separation chamber; 19. a separated particle collection chamber.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Referring to fig. 1-4, an embodiment of the present invention is shown: a magnetic bead separation device for separating adeno-associated virus particles comprises a magnetic bead separation box 13, wherein a magnetic bead separation cavity 18 and a separated particle collection cavity 19 are arranged inside the magnetic bead separation box 13, the magnetic bead separation cavity 18 and the separated particle collection cavity 19 are used for separating magnetic beads and collecting separated particles respectively, a magnet supporting plate 17 is arranged at the bottom end of an inner cavity of the magnetic bead separation cavity 18, the separated magnetic beads are adsorbed on the magnet supporting plate 17, a scraper 16 is arranged at one end of the top of the magnet supporting plate 17, one end of the scraper 16 is connected with a telescopic end of a second electric push rod 8, and the scraper 16 can scrape the magnetic beads at the top of the magnet supporting plate 17, so that the magnetic beads are separated from the magnet supporting plate 17 and are discharged from a magnetic bead discharge port 11;
magnetic bead separation chamber 18's top one side is equipped with feed head 7, and is equipped with top cap 1 directly over feed head 7, and top cap 1's bottom middle part is equipped with tongue 2, can automatic control top cap 1 seal on feed head 7 through first electric push rod 6, and the tongue 2 embedding is in feed head 7, and the bottom both sides of top cap 1 all inlay fixedly with the flexible end of first electric push rod 6.
Further, the middle part of top cap 1 is equipped with motor 3, and the one end of motor 3 is equipped with stirring frame 4, and the both sides of stirring frame 4 all are equipped with stirring leaf 5, rotate through motor 3 control stirring frame 4, make stirring frame 4 carry out the centrifugal stirring through the stirring leaf 5 in its outside to the granule.
Further, a material conveying groove is formed between the magnetic bead separation cavity 18 and the separated particle collection cavity 19, a material discharging head 14 is arranged at a position below one end of the separated particle collection cavity 19, a material taking opening 15 is formed in the top of the separated particle collection cavity 19, a sealing cover 12 is installed on the material taking opening 15, the separated particles are conveyed into the separated particle collection cavity 19 through the material conveying groove, and the separated particles are taken out through the material discharging head 14.
Further, the cylinder body of first electric putter 6 is inlayed in the inside of magnetic bead separator box 13, and the bottom both sides of top cap 1 all are equipped with inlays the groove, and the leading end of cylinder body can be embedded in inlaying the inslot, not only conveniently is connected with the one end of first electric putter 6 through inlaying the groove, and can accomodate the cylinder body after drawing in.
Further, a material guide cavity with an electromagnetic valve is arranged on one side of the magnetic bead separation cavity 18, a magnetic bead discharge port 11 is arranged on the front surface of the material guide cavity, and magnetic beads are conveyed to the magnetic bead discharge port 11 through the material guide cavity and taken out.
Further, the bottom of magnetic bead separator box 13 is equipped with bracket 9, and the bottom of bracket 9 is equipped with stabilizing base 10, makes things convenient for holding of magnetic bead separator box 13 fixed through bracket 9, and can make magnetic bead separator box 13 carry out the stable stay through stabilizing base 10 and place.
The working principle is as follows: when the device is used, virus particles are added into the magnetic bead separation cavity 18 through the feeding head 7, the first electric push rod 6 controls the top cover 1 to be pressed downwards, the convex groove 2 at the bottom end of the top cover 1 is embedded and sealed with the feeding head 7, the stirring frame 4 enters the magnetic bead separation cavity 18, the motor 3 controls the stirring frame 4 to rotate, the stirring frame 4 carries out centrifugal stirring on the particles, the magnetic beads marked on the magnetic bead are adsorbed to the magnetic field of the magnet supporting plate 17, the magnetic beads are separated, the separated particles are conveyed into the separated particle collection cavity 19 through the conveying groove, then the discharge head 14 is discharged, after separation is completed, the second electric push rod 8 controls the scraper 16 to scrape the magnet supporting plate 17, the magnetic beads adsorbed on the magnetic bead are pushed to one end of discharge, and the electromagnetic valve is opened to be discharged through the magnetic bead discharge port 11.
It will be evident to those skilled in the art that the utility model is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (6)
1. A magnetic bead separator for separating adeno-associated virus particles, includes magnetic bead separator case (13), its characterized in that: a magnetic bead separation cavity (18) and a separated particle collection cavity (19) are arranged inside the magnetic bead separation box (13), a magnet supporting plate (17) is arranged at the bottom end of an inner cavity of the magnetic bead separation cavity (18), a scraper (16) is arranged at one end of the top of the magnet supporting plate (17), and one end of the scraper (16) is connected with the telescopic end of the second electric push rod (8);
the magnetic bead separation chamber is characterized in that a feeding head (7) is arranged on one side of the top end of the magnetic bead separation chamber (18), a top cover (1) is arranged right above the feeding head (7), a convex groove (2) is arranged in the middle of the bottom end of the top cover (1), the convex groove (2) is embedded into the feeding head (7), and the two sides of the bottom of the top cover (1) are embedded and fixed with the telescopic end of the first electric push rod (6).
2. A magnetic bead separation device for separating adeno-associated virus particles according to claim 1, wherein: the middle part of top cap (1) is equipped with motor (3), and the one end of motor (3) is equipped with stirring frame (4), the both sides of stirring frame (4) all are equipped with stirring leaf (5).
3. A magnetic bead separation device for separating adeno-associated virus particles according to claim 1, wherein: the magnetic bead separation chamber (18) and the granule after the separation are collected and are equipped with the defeated silo between chamber (19), the one end of the granule after the separation is collected chamber (19) and is located to be equipped with row stub bar (14) down, the top of the granule after the separation is collected chamber (19) is equipped with gets material mouth (15), and gets and install sealed lid (12) on material mouth (15).
4. A magnetic bead separation device for separating adeno-associated virus particles according to claim 1, wherein: the cylinder body of first electric putter (6) is inlayed in the inside of magnetic bead separator box (13), the bottom both sides of top cap (1) all are equipped with inlays the groove, and the proud of cylinder body can be embedded in inlaying the inslot.
5. A magnetic bead separation device for separating adeno-associated virus particles according to claim 1, wherein: a material guide cavity with an electromagnetic valve is arranged on one side of the magnetic bead separation cavity (18), and a magnetic bead discharge port (11) is arranged on the front surface of the material guide cavity.
6. A magnetic bead separation device for separating adeno-associated virus particles according to claim 1, wherein: the bottom of magnetic bead separator box (13) is equipped with bracket (9), and the bottom of bracket (9) is equipped with stabilizing base (10).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122174140.9U CN215757393U (en) | 2021-09-09 | 2021-09-09 | Magnetic bead separation device for separating adeno-associated virus particles |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202122174140.9U CN215757393U (en) | 2021-09-09 | 2021-09-09 | Magnetic bead separation device for separating adeno-associated virus particles |
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| CN215757393U true CN215757393U (en) | 2022-02-08 |
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| CN202122174140.9U Active CN215757393U (en) | 2021-09-09 | 2021-09-09 | Magnetic bead separation device for separating adeno-associated virus particles |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114196638A (en) * | 2021-12-28 | 2022-03-18 | 武汉美博尔津生物科技有限公司 | Adeno-associated virus purification kit and purification method |
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2021
- 2021-09-09 CN CN202122174140.9U patent/CN215757393U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114196638A (en) * | 2021-12-28 | 2022-03-18 | 武汉美博尔津生物科技有限公司 | Adeno-associated virus purification kit and purification method |
| CN114196638B (en) * | 2021-12-28 | 2023-12-15 | 武汉美博尔津生物科技有限公司 | Adeno-associated virus purification kit and purification method |
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