CN215103239U

CN215103239U - A new type of PBMC cell separation tube

Info

Publication number: CN215103239U
Application number: CN202121264297.4U
Authority: CN
Inventors: 康恺; 李佐青; 陈大为; 刘津
Original assignee: Chuangmo Biotechnology Beijing Co ltd
Current assignee: Chuangmo Biotechnology Beijing Co ltd
Priority date: 2021-06-07
Filing date: 2021-06-07
Publication date: 2021-12-10
Anticipated expiration: 2031-06-07

Abstract

The utility model relates to the technical field of medicine, in particular to a novel PBMC cell separating tube, which comprises a separating tube and a rotating cover, wherein the surface of the separating tube is close to the top end and the inner wall of the rotating cover is provided with coaxial threads, the rotating cover is meshed with the coaxial threads on the separating tube, the rotating cover is sealed and sheathed on the top end of the separating tube, the top end of the rotating cover is provided with a liquid injection hole, a first isolation funnel is arranged in the separating tube, the bottom end of the isolation funnel is provided with a through hole, the bottom end of the through hole is provided with a second isolation funnel, the surface of the first isolation funnel is provided with a leak hole, the surface of the separating tube is provided with two groups of limiting fixture blocks, the surface of the separating tube is provided with a spring, one end of the spring is connected with the limiting fixture blocks, the surfaces of the separating tube and the limiting fixture blocks are provided with limiting retainer rings, the surfaces of the retainer rings are inclined inwards, the surface of the separating tube is provided with scales, and the device can rapidly add a blood sample to the liquid level of the separating liquid, and can prevent the mixing of the blood liquid layer and the separation liquid.

Description

Novel PBMC cell separating tube

Technical Field

The utility model relates to a medical science technical field specifically is a novel PBMC cell separator tube.

Background

Peripheral blood mononuclear cells are mainly lymphocytes, and are the most commonly used cell population in immunological experiments. Monocytes are a more commonly used cell in basic and clinical in vitro studies. How to rapidly and effectively separate high-purity mononuclear cells from peripheral blood is a problem which is worth to be discussed. The traditional method for separating the mononuclear cells is a density gradient separation method, but the difficulty of density gradient stratification is as follows: too fast, the unbalanced or unclear layering that opens the brake of centrifuging process application of sample, centrifuging tube arouses all can influence the experiment effect, and the separation process is loaded down with trivial details, and the time spent is too long.

For example, the patent publication "CN 204022830U" is named as: the patent of "a cell separation pipe for separating and draw PBMC", includes tube cap and body, the coaxial screw thread lid of tube cap closes and installs in the top of body, the coaxial sealing installation isolation layer of well lower part in the body, the isolation layer is open-top's hollow hourglass hopper-shaped, and the bottom of this isolation layer is the toper bottom, the hole is annotated to the coaxial setting in bottom center of isolation layer.

This a cell separation pipe for separating and draw PBMC, when using, single isolation layer makes too slow that parting liquid and blood model fuse, great promotion the time of separation, and the cold-resistant and heat-resisting degree of this body is low excessively, makes the not thorough inadequately of separation easily.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

Aiming at the defects of the prior art, the utility model provides a novel PBMC cell separating tube.

(II) technical scheme

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a novel PBMC cell separating tube, includes the separator tube and changes the lid, the separator tube surface sets up coaxial screw with changeing the lid inner wall, change the coaxial screw intermeshing on lid and the separator tube, just it adorns on the separator tube top to change the lid seal, it sets up the notes liquid hole to change the lid top, set up first isolation funnel in the separator tube, keep apart funnel bottom equipartition through-hole, the through-hole bottom sets up the second and keeps apart the funnel, first isolation funnel surface equipartition small opening, the separator tube surface sets up two sets of spacing fixture blocks, the separator tube surface sets up the spring, spring one end is connected with spacing fixture block, separator tube and spacing cardboard surface set up spacing rand, spacing rand surface leanin, the separator tube surface sets up the scale.

In order to increase separator tube intensity, the utility model discloses the improvement has, the separator tube inner wall sets gradually heat-resisting layer, winter protection layer and antiseized layer, just the separator tube inner wall sets up the strengthening rib.

In order to facilitate the increase of the heat resistance and the cold resistance of the separation tube, the utility model discloses the improvement has, antiseized layer, heat-resisting layer and winter protection layer material are sodium carboxymethyl cellulose coating, aerogel felt and polyethylene insulation material respectively.

In order to facilitate the fixing of the separation tube, the utility model discloses the improvement has, the separation tube bottom is circular-arc.

In order to facilitate the isolation of the blood sample plate, the utility model discloses the improvement has, the diameter of small opening is 1 ~ 1.5 mm.

In order to look over the capacity of blood and separating medium, the utility model discloses the improvement has, the scale is 10ml scale dress volume, just first isolation funnel is located the 3ml department of separating tube inner wall.

(III) advantageous effects

Compared with the prior art, the utility model provides a novel PBMC cell separator tube possesses following beneficial effect:

this novel PBMC cell separation tube, through the cooperation of first isolation funnel, second isolation funnel and small opening, can add the blood sample to the liquid level of parting liquid fast to can prevent the mixture of blood liquid layer and parting liquid, through the cooperation on antiseized layer, heat-resistant layer and winter protection layer, can increase separator tube heat resistance and cold resistance, and antiseized layer can prevent the liquid adhesion at the separator tube inner wall.

Drawings

Fig. 1 is a 3D diagram of the structure of the present invention;

FIG. 2 is a front view of the structure of the present invention;

fig. 3 is a top view of a first isolation funnel structure of the present invention;

fig. 4 is a sectional view of the inner wall structure of the separation tube according to the present invention.

In the figure: 1. a separation tube; 2. rotating the cover; 3. coaxial threads; 4. a liquid injection hole; 5. a first isolation funnel; 6. a second isolation funnel; 7. a leak hole; 8. a limiting clamping block; 9. a spring; 10. a limiting retainer ring; 11. calibration; 12. an anti-sticking layer; 13. a heat-resistant layer; 14. a cold-proof layer; 15. and (5) reinforcing ribs.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-4, a novel PBMC cell separation tube 1 includes a separation tube 1 and a rotary cover 2, and is characterized in that: the utility model discloses a separation tube, including separator tube 1, rotary cover 2, separating tube 1, rotary cover 2 and the coaxial screw thread 3 intermeshing on the separator tube 1, just rotary cover 2 seals suit in 1 top of separator tube, 2 tops of rotary cover set up annotate liquid hole 4, set up first isolation funnel 5 in the separator tube 1, first 5 bottom equipartition through-holes of isolation funnel, funnel 6 is kept apart to a plurality of second in through-hole bottom, 5 surperficial equipartition small openings 7 of first isolation funnel, separator tube 1 surface sets up two sets of spacing fixture blocks 8, separator tube 1 surface sets up spring 9, spring 9 one end is connected with spacing fixture block 8, separator tube 1 and spacing cardboard surface set up spacing rand 10, spacing rand 10 surface leanin, separator tube 1 surface sets up scale 11.

1 inner wall of separator tube sets gradually heat-resistant layer 13, winter protection layer 14 and antiseized layer 12, just 1 inner wall of separator tube sets up strengthening rib 15, can increase 1 heat resistance of separator tube and cold resistance through setting up heat-resistant layer 13 and winter protection layer 14, and antiseized layer 12 can prevent the liquid adhesion at 1 inner wall of separator tube.

The anti-sticking layer 12, the heat-resistant layer 13 and the cold-proof layer 14 are made of sodium carboxymethyl cellulose coating, aerogel felt and polyethylene heat-insulating materials, and the sodium carboxymethyl cellulose coating, the aerogel felt and the polyethylene heat-insulating materials can reduce the occupied area of the materials and enhance the strength of the separation pipe 1.

The bottom of the separation tube 1 is arc-shaped, and the bottom end of the separation tube 1 is arc-shaped, so that the separation tube 1 can be conveniently placed on a reagent rack.

The diameter of the leak hole 7 is 1-1.5 mm, and the separation of the separation liquid and the blood sample plate is facilitated by controlling the diameter of the leak hole 7.

The scale 11 is the 11 volume of 10ml scale, just first isolation funnel 5 is located the 3ml department of separator tube 1 inner wall, can be convenient for control the weight that the separator poured into separator tube 1 in through setting up first isolation funnel 5 in separator tube 13ml department.

To sum up, when the novel PBMC cell separation tube 1 is used, the separation liquid with the pre-filled component enters the separation tube 1 through the liquid injection hole 4 and then enters the bottom end of the separation tube 1 through the first isolation funnel 55, so that the separation liquid is fully precipitated at the bottom end of the separation tube 1, the blood sample is injected into the separation tube 1 through the liquid injection hole 4, and the separation tube 1 is placed in a centrifuge for separation; after separation, liquid stratification is presented in the cell separation tube 1 due to different densities, wherein the red blood cells are granulocytes, separation liquid, mononuclear cells (leucocyte layer) and diluted blood plasma which are sequentially arranged at the bottommost layer, the leucocyte layer is the mononuclear cells to be extracted, the separated PBMC can be directly poured into a new centrifugal tube easily after centrifugation, the diluted liquid is used for cleaning twice, and the PBMC required to be extracted is deposited at the bottommost part of the test tube and taken out. And the design of first isolation funnel 5 and second isolation funnel 6 can add the blood sample to the liquid level of parting liquid fast, and can prevent the mixture of liquid layer and parting liquid, when centrifugation, need not to close centrifuge brake, when needs place separator tube 1 on the test-tube rack, make 8 bottoms of spacing fixture block pass the test-tube rack, and spring 9 can increase elasticity to spacing fixture block 8, make separator tube 1 be convenient for fix the slope of placing the test-tube rack on the test-tube rack, and spacing rand 10 can further fixed separator tube 1.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A novel PBMC cell separation tube comprises a separation tube (1) and a rotary cover (2), and is characterized in that: the separation tube (1) surface and the inner wall of the rotary cover (2) are provided with coaxial threads (3), the rotary cover (2) is meshed with the coaxial threads (3) on the separation tube (1), the rotary cover (2) is sleeved on the top end of the separation tube (1) in a sealing manner, the top end of the rotary cover (2) is provided with a liquid injection hole (4), a first isolation funnel (5) is arranged in the separation tube (1), through holes are uniformly distributed at the bottom end of the first isolation funnel (5), a plurality of second isolation funnels (6) are arranged at the bottom end of the through holes, leakage holes (7) are uniformly distributed on the surface of the first isolation funnel (5), two groups of limiting clamping blocks (8) are arranged on the surface of the separation tube (1), a spring (9) is arranged on the surface of the separation tube (1), one end of the spring (9) is connected with the limiting clamping blocks (8), and limiting clamping rings (10) are arranged on the surfaces of the separation tube (1) and the limiting clamping plates, the surface of the limiting clamping ring (10) inclines inwards, and scales (11) are arranged on the surface of the separation pipe (1).

2. The novel PBMC cell separation tube according to claim 1, wherein: the inner wall of the separation pipe (1) is sequentially provided with a heat-resistant layer (13), a cold-proof layer (14) and an anti-sticking layer (12), and the inner wall of the separation pipe (1) is provided with a reinforcing rib (15).

3. The novel PBMC cell separation tube according to claim 2, wherein: the anti-sticking layer (12), the heat-resistant layer (13) and the cold-proof layer (14) are respectively made of a sodium carboxymethyl cellulose coating, an aerogel felt and a polyethylene heat-insulating material.

4. The novel PBMC cell separation tube according to claim 3, wherein: the bottom of the separation pipe (1) is arc-shaped.

5. The novel PBMC cell separation tube according to claim 1, wherein: the diameter of the leak hole (7) is 1-1.5 mm.

6. The novel PBMC cell separation tube according to claim 1, wherein: the scale (11) is 10ml of scale loading amount, and the first isolation funnel (5) is positioned at 3ml of the inner wall of the separation tube (1).