CN215088029U - High-purity platelet continuous separation device - Google Patents

Abstract

The utility model discloses a high-purity platelet continuous separation device aims at the continuous type platelet separator that platelet separation purity, concentration and collection efficiency are high that provides. The utility model discloses a following technical scheme realizes: a high purity platelet continuous separation device comprising: the tubular centrifuge that is equipped with once centrifugal district and secondary centrifugal district reaches the elasticity that is connected with tubular centrifuge and moves back the bundle of twisting, anticoagulant whole blood gets into and carries out the first separation of platelet in the first centrifugal district, and the platelet that separates once in the first centrifugal district is transferred to the secondary centrifugal district and is carried out purification separation once more, takes out earlier impurity cell from the secondary centrifugal district and gets back to the first centrifugal district, collects purification platelet from the secondary centrifugal district again.

Description

High-purity platelet continuous separation device

Technical Field

The utility model relates to a separation device mainly used for separating blood components, in particular to a separation device for single blood collecting platelets and high-purity platelets.

Background

Apheresis is a new blood transfusion technique developed in recent decades with the progress of medical science and medical technology. The emergence and development of apheresis is a milestone in the advancement of modern transfusion, with apheresis being significantly superior to whole blood transfusion. Transfusions of blood components have the following advantages over transfusions of whole blood: 1. high purity and good curative effect; 2. safe for transfusion and has small side effect; 3. the stability is good, and the storage and the transportation are convenient; 4. comprehensive utilization and blood saving.

Human blood is composed of red blood cells, white blood cells, platelets, plasma, etc. Component blood donation is to separate and extract the designated blood components from the blood of the donor by using a blood component separation device, and to return the rest components to the donor. In GB 18469-2012 Whole blood and component blood quality requirement, higher requirements are set for the platelet apheresis. Besides the platelet concentration of the collected platelets and the volume of the finished product, the mixing amount of red blood cells and white blood cells is also required. Currently, the common platelet apheresis devices on the market mainly include a cup type centrifugal separation device represented by MCS + product of american blood technologies and a bag type centrifugal separation device represented by Trima product of talocene, japan, and the above separation devices have the following problems and disadvantages:

1. cup-type centrifugal separation device:

a cup type centrifugal separation device belongs to an intermittent centrifugal separation device, and the separation device adopts a fixed volume type centrifugal cup as a centrifugal separator. The fixed volume centrifuge cup has two ports, one of which is an inlet for anticoagulated whole blood and the other of which is an outlet for constituent blood. The working principle is that the anticoagulated whole blood enters the rotary fixed volumetric centrifugal cup from the anticoagulated whole blood inlet, various components in the anticoagulated whole blood are layered under the action of centrifugal force due to different densities, and the components sequentially flow out of a component blood outlet from low-density component blood to high-density component blood when cells are fully stacked in the centrifugal cup. Because the position of the blood cell layered interface can not be dynamically and freely adjusted in the process of one round of collection, and the positions of the blood component overflow port and the blood layered interface are not parallel, the blood component separation has to be carried out by adopting modes such as elutriation and surfing and the like so as to obtain the platelet meeting the quality control requirement. It has the defects of overlarge blood circulation of donors in vitro, high qualification rate of platelet apheresis, low efficiency and the like.

2. Bag-type centrifugal separation apparatus:

the bag type centrifugal separator belongs to the field of continuous centrifugal separator, and is one ring centrifugal bag. The annular centrifuge bag typically has four ports, one of which is an inlet for anticoagulated whole blood and the remaining three of which are outlets for three-component blood. The working principle is that the anticoagulated whole blood enters a rotating centrifugal bag, various components in the anticoagulated whole blood are layered under the action of centrifugal force due to different densities, and layered component blood flows out from a corresponding component blood outlet. Because the layering interface area of blood cells is overlarge in the separation process, the thickness of each component blood cell layering area is very thin, and the quality control requirement of component blood collection cannot be met by adopting a conventional method to extract the layering cells from the layering pipe orifice. In order to meet the collection quality control requirement of collected component blood, the LRS centrifugal bin is used for secondary centrifugal separation. The method can cause a great amount of white blood cells and platelets of the donor to be stuck in the LRS centrifugal bin and cannot be returned to the donor, and has the problems and defects of low platelet collection rate and great loss of white blood cells of the donor.

In order to meet the requirement of collecting high-purity platelets, the high-purity platelet separation device which achieves better single-platelet purity, purity and collection efficiency simultaneously meets the following technical characteristics:

1. the area of the blood component stratification interface is small enough to make the thickness of the blood component cell stratification region larger;

2. the purity and concentration of the platelets can be improved by secondary centrifugation and re-separation;

3. the layered interface position of each component blood can be dynamically adjusted by adjusting the flow rate of the anticoagulated whole blood flowing into the centrifuge and the flow rate of the component blood flowing out of the centrifuge, so that the aim of collecting the target component blood or gathering the target component blood cells in the centrifuge is fulfilled;

4. the component blood collecting port is parallel or approximately parallel to each component blood layering interface;

5. when the component blood is collected, a certain centrifugal force is required to be ensured at the corresponding component blood outlet so as to maintain the stability of the component blood layered interface;

6. after separation, the component blood in the centrifuge can be discharged out of the centrifuge and returned to the donor, and the residue is small enough;

7. safe feedback emptying measures are provided;

8. the centrifuge can continuously rotate along a certain axis, and anticoagulated whole blood entering the centrifuge is layered under the action of centrifugal force;

9. anticoagulated whole blood can continuously flow into the centrifuge, and non-collected target component blood can continuously extrude out of the centrifuge and can be safely returned to a human body.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the problems and shortcomings existing in the prior art and providing a high-purity continuous platelet separation device.

The utility model discloses a following technical scheme realizes above-mentioned purpose: a high purity platelet continuous separation device comprising: be equipped with tubular centrifuge 1 of once centrifugal district 2 and secondary centrifugal district 3 and the elasticity of being connected with tubular centrifuge 1 and move back and turn round tube bank 10, its characterized in that: the primary centrifugal area 2 and the secondary centrifugal area 3 are mutually isolated, an anticoagulated whole blood vessel 14, a component blood vessel 15 and a erythrocyte tube 16 are fixed and inserted into the primary centrifugal area 2 of the tubular centrifuge 1, the anticoagulated whole blood vessel 14 is provided with an anticoagulated whole blood inlet 4 in the primary centrifugal area 2, the component blood vessel 15 is provided with a component blood outlet 5 in the primary centrifugal area 2, the erythrocyte tube 16 is provided with an erythrocyte outlet 6 in the primary centrifugal area 2, the anticoagulated whole blood inlet 4, the component blood outlet 5 and the erythrocyte outlet 6 are all positioned at the same side of a centrifugal rotation central line 21 in the centrifugal rotation process, and the vertical distances from the centrifugal rotation central line 21 are the erythrocyte outlet 6, the anticoagulated whole blood inlet 4 and the component blood outlet 5 from large to small in sequence, the erythrocyte outlet 6 is close to the tube bottom 50 of the primary centrifugal area, the secondary centrifugal area 3 is at least provided with a transfer tube one 17 and a transfer tube three transfer tube 18, the transfer tube one 17, the transfer tube one transfer tube 17, the component blood outlet and the transfer tube outlet 5, The third transfer tube 18 is fixed and inserted into the secondary centrifugal area 3 of the tubular centrifuge 1, the first transfer tube 17 is provided with a first transfer port 7 in the secondary centrifugal area 3, the third transfer tube 18 is provided with a third transfer port 8 in the secondary centrifugal area 3, the first transfer port 7 and the third transfer port 8 are both positioned on the same side of the centrifugal rotation central line 21 in the centrifugal rotation process, the first transfer port 7 is close to the tube bottom 51 of the secondary centrifugal area, the vertical distance from the first transfer port 7 to the centrifugal rotation central line 21 is greater than the vertical distance from the third transfer port 8 to the centrifugal rotation central line 21, the anticoagulation whole blood vessel 14, the component blood vessel 15, the erythrocyte tube 16, the first transfer tube 17 and the third transfer tube 18 are converged into an elastic untwisted tube bundle 10 on the same side of the outside of the tubular centrifuge 1, and the elastic untwisted tube bundle fixing head 11 is arranged on the elastic untwisted tube bundle 10.

The utility model discloses compare and have following beneficial effect in prior art:

the quality of the platelet finished product is stable. The utility model discloses a tubular centrifuge 1 realizes anti-freezing whole blood separation in tubular centrifuge 1 along the rotatory mode of centrifugal rotation central line 21, effectively reduces blood layering interfacial area, improves layering interface thickness, has reduced the possibility that other composition blood got into secondary centrifugation district 3, and the finished product stable in quality is collected to the platelet.

The platelet collection purity is high. The utility model discloses a method of taking out earlier in the secondary centrifugation district 3 foreign cell and carrying out the platelet collection again can effectively get rid of foreign cell, improves platelet and collects purity and concentration.

The donor cells are less lost. The utility model discloses a keep tubular centrifuge 1 rotatory along centrifugal rotation center line 21, to the method of pouring into the air in tubular centrifuge 1, can effectively empty among the tubular centrifuge 1 composition blood and realize the feedback, reduced the loss of donor's cell.

The platelet collection rate is high. The utility model discloses the platelet that is contained in the foreign matter cell who takes out in the secondary centrifugation district 3 gets into once again through anticoagulation whole blood vessel 14 and separates once more in centrifugation district 2, and the platelet all can not the feedback to human 44 in tubular centrifuge 1 in whole continuous collection in-process, has improved the platelet collection rate.

Drawings

FIG. 1 is a schematic cross-sectional view of a high-purity platelet continuous separation apparatus according to the present invention;

FIG. 2 is a schematic view of the rotational untwisting principle of FIG. 1;

FIG. 3 is a schematic diagram of the connection of the single needle and single blood platelet embodiment of FIG. 1;

FIG. 4 is a schematic diagram illustrating the evacuation flow of the return line in the single needle and single blood platelet embodiment of FIG. 1;

FIG. 5 is a schematic diagram of a blood inlet centrifugation stratification procedure in the single needle platelet apheresis embodiment of FIG. 1;

FIG. 6 is a schematic diagram of the in-process return flow of the single needle single blood platelet embodiment of FIG. 1;

FIG. 7 is a schematic view illustrating the flow of platelets transferred to the secondary centrifugation chamber in the single-needle single-platelet embodiment of FIG. 1;

FIG. 8 is a schematic diagram illustrating the plasma flushing process for preparing the secondary centrifuge chamber in the single needle single platelet embodiment of FIG. 1;

FIG. 9 is a schematic diagram of a process for removing foreign cells in the single needle and single blood platelet embodiment of FIG. 1;

FIG. 10 is a schematic diagram of a platelet collection procedure in the single needle and single platelet embodiment of FIG. 1;

FIG. 11 is a schematic diagram of the collection completion feedback flow in the single needle single blood platelet embodiment of FIG. 1;

FIG. 12 is a schematic cross-sectional view of a high-purity platelet continuous separation apparatus according to the present invention with a platelet collection tube installed independently;

FIG. 13 is a schematic diagram of the connection of the single needle platelet apheresis embodiment of FIG. 12;

FIG. 14 is a schematic diagram illustrating a return line evacuation flow in the single needle platelet collection embodiment of FIG. 12;

FIG. 15 is a schematic diagram of a blood inlet centrifugation stratification procedure in the single needle platelet apheresis embodiment of FIG. 12;

FIG. 16 is a schematic diagram of the in-process return flow of the single needle single blood platelet embodiment of FIG. 12;

FIG. 17 is a schematic diagram illustrating the flow of platelets transferred to the secondary centrifugation chamber in the single needle platelet apheresis embodiment of FIG. 12;

FIG. 18 is a schematic diagram of a plasma flow scheme for preparing a secondary centrifuge chamber flush in the single needle platelet apheresis embodiment of FIG. 12;

FIG. 19 is a schematic diagram of a process for removing contaminating cells in the single needle platelet apheresis embodiment of FIG. 12;

FIG. 20 is a schematic diagram of the procedure for collecting high purity platelets in the single needle platelet apheresis embodiment of FIG. 12;

FIG. 21 is a schematic diagram of a collection completion return flow in the single needle platelet collection embodiment of FIG. 12;

FIG. 22 is a schematic cross-sectional view of a simplified tube clamp valve implementation of a high purity platelet continuous separation device of the present invention;

FIG. 23 is a schematic diagram of the connection of the single needle platelet apheresis embodiment of FIG. 22;

FIG. 24 is a schematic diagram illustrating the return line evacuation flow in the single needle platelet collection embodiment of FIG. 22;

FIG. 25 is a schematic diagram of a blood inlet centrifugation slice flow in the single needle platelet apheresis embodiment of FIG. 22;

FIG. 26 is a schematic diagram of the in-process return flow of the single needle single blood platelet embodiment of FIG. 22;

FIG. 27 is a schematic view of the flow of platelets transferred to the secondary centrifugation chamber in the single needle platelet apheresis embodiment of FIG. 22;

FIG. 28 is a schematic diagram of a plasma flow scheme for preparing a secondary centrifuge chamber flush in the single needle platelet apheresis embodiment of FIG. 22;

FIG. 29 is a schematic diagram of the flow of contaminant cell removal in the single needle platelet apheresis embodiment of FIG. 22;

FIG. 30 is a schematic diagram of the procedure for collecting high purity platelets in the single needle platelet apheresis embodiment of FIG. 22;

FIG. 31 is a schematic diagram of a collection completion return flow in the single needle platelet collection embodiment of FIG. 22;

in the figure: 1 tubular centrifuge, 2 primary centrifugation zone, 3 secondary centrifugation zone, 4 anticoagulated whole blood inlet, 5 component blood outlet, 6 erythrocyte outlet, 7 transfer port I, 7-1 transfer port II, 8 transfer port III, 9 platelet collection port, 10 elastic untwisted tube bundle, 11 elastic untwisted tube bundle fixing head, 12 centrifuge untwisted stent, 13 centrifugal disk motor, 14 anticoagulated whole blood vessel, 15 component blood vessel, 16 erythrocyte tube, 17 transfer tube I, 17-1 transfer tube II, 18 transfer tube III, 19 platelet collection tube, 20 centrifugal disk, 21 centrifugal rotation center line, 22 untwisted rotation center line, 23 anticoagulated whole blood peristaltic pump, 24 anticoagulant peristaltic pump, 25 feedback peristaltic pump, 26 transfer peristaltic pump, 27 pinch valve I, 28 pinch valve II, 29 pinch valve III, 30 pinch valve IV, 31 pinch valve V, 32 buffer bag, 33 platelet collection bag, 34 buffer bag lower level sensor, 35 buffer bag upper liquid level sensor, 36 blood component sensor, 37 impurity cell tube, 38 buffer bag communicating tube, 39 anticoagulant bag, 40 feedback tube, 41 whole blood communicating tube, 42 anticoagulant tube, 43 puncture needle, 44 human body, 45 red blood cell, 46 white blood cell, 47 platelet, 48 plasma, 49 frame, 50 primary centrifugal zone tube bottom, 51 secondary centrifugal zone tube bottom.

Detailed Description

The first specific embodiment is as follows:

see fig. 1. In a first embodiment described below, a high purity platelet continuous separation device includes: be equipped with tubular centrifuge 1 of once centrifugal district 2 and secondary centrifugal district 3 and the elasticity of being connected with tubular centrifuge 1 and move back and turn round tube bank 10, its characterized in that: the primary centrifugal area 2 and the secondary centrifugal area 3 are isolated from each other, an anticoagulated whole blood vessel 14, a component blood vessel 15 and a erythrocyte tube 16 are fixed and inserted into the primary centrifugal area 2 of the tubular centrifuge 1, the anticoagulated whole blood vessel 14 is provided with an anticoagulated whole blood inlet 4 in the primary centrifugal area 2, the component blood vessel 15 is provided with a component blood outlet 5 in the primary centrifugal area 2, the erythrocyte tube 16 is provided with an erythrocyte outlet 6 in the primary centrifugal area 2, the anticoagulated whole blood inlet 4, the component blood outlet 5 and the erythrocyte outlet 6 are all positioned at the same side of a centrifugal rotation central line 21 in the centrifugal rotation process, the vertical distances from the centrifugal rotation central line 21 are the erythrocyte outlet 6, the anticoagulated whole blood inlet 4 and the component blood outlet 5 from large to small in turn, the erythrocyte outlet 6 is close to the tube bottom 50 of the primary centrifugal area, a transfer tube I17 and a transfer tube III 18 are fixed and inserted into the secondary centrifugal area 3 of the tubular centrifuge 1, the first transfer tube 17 is provided with a first transfer port 7 in the secondary centrifugal area 3, the third transfer tube 18 is provided with a third transfer port 8 in the secondary centrifugal area 3, the first transfer port 7 and the third transfer port 8 are both positioned on the same side of the centrifugal rotation central line 21 in the centrifugal rotation process, the first transfer port 7 is close to the tube bottom 51 of the secondary centrifugal area, the vertical distance from the first transfer port 7 to the centrifugal rotation central line 21 is greater than the vertical distance from the third transfer port 8 to the centrifugal rotation central line 21, the anticoagulation whole blood vessel 14, the component blood vessel 15, the erythrocyte tube 16, the first transfer tube 17 and the third transfer tube 18 are converged into an elastic untwisted tube bundle 10 on the same side outside the tube centrifuge 1, and the elastic untwisted tube bundle 10 is provided with an elastic untwisted tube bundle fixing head 11.

Preferably, all tubes are hard tubes inside the tube centrifuge 1 in order to ensure that the tubes inside the tube centrifuge 1 do not shake during centrifugation; to ensure smooth untwisting, all tubes are provided with flexible tubes outside the tube centrifuge 1.

Preferably, the blood component outlet 5 is provided at the position where the vertical distance from the primary centrifugal region 2 to the centrifugal rotation center line 21 is minimum in order to ensure complete evacuation of the air in the primary centrifugal region 2, and the transfer port three 8 is provided at the position where the vertical distance from the secondary centrifugal region 3 to the centrifugal rotation center line 21 is minimum in order to ensure complete evacuation of the air in the secondary centrifugal region 3.

See fig. 2. The centrifugal disc motor 13 is connected with the centrifugal disc 20, the centrifugal disc 20 is provided with a centrifuge back-twist support 12 fixed on the outer ring of a bearing, the centrifugal disc motor 13 is fixed on a frame 49, an elastic back-twist tube bundle fixing head 11 on an elastic back-twist tube bundle 10 is fixed on the frame 49, and the tubular centrifuge 1 is inserted into the centrifuge back-twist support 12 with the bearing and forms a tight fit with the inner ring of the bearing. When the centrifugal disc motor 13 rotates, the centrifugal back-twist bracket 12 on the centrifugal disc 20 drives the tubular centrifuge 1 to rotate along the centrifugal rotation central line 21, and simultaneously, due to the elastic stress existing on the elastic back-twist tube bundle 10, the tubular centrifuge 1 is driven to rotate and back-twist along the back-twist rotation central line 22, so that the device can continuously rotate and centrifuge without knotting.

See fig. 3. The anticoagulated whole blood vessel 14 is clamped on the anticoagulated whole blood peristaltic pump 23 and connected with a whole blood communicating pipe 41, the erythrocyte tube 16 is connected with a buffer bag 32, the horizontal position of a connecting port is not lower than the liquid level sensor 35 on the buffer bag, the component blood tube 15 is clamped on the transfer peristaltic pump 26 and provided with a blood component sensor 36, the component blood tube 15 is communicated with a transfer tube I17 and provided with a pinch valve I27 at the communicating position, the component blood tube 15 is communicated with a transfer tube III 18 and provided with a pinch valve III 29 at the communicating position, the transfer tube I17 is communicated with the impurity cell tube 37 and provided with a pinch valve II 28 at the communicating position, the transfer tube III 18 is communicated with a buffer bag communicating pipe 38 and provided with a pinch valve IV 30 at the communicating position, the transfer tube I17 is communicated with the platelet collecting bag 33 and provided with a pinch valve V31, each pinch valve is a pipeline which is not communicated with two ends of the platelet collecting bag when being closed, the pipelines connected with two ends of the transfer tube communicating pipe when the transfer tube, the impurity cell tube 37 is communicated with the anticoagulated whole blood vessel 14, the buffer bag communicating pipe 38 is communicated with the buffer bag 32, the horizontal position of the connector is not lower than that of the buffer bag upper liquid level sensor 35, the buffer bag 32 is provided with the buffer bag lower liquid level sensor 34 and the buffer bag upper liquid level sensor 35, one end of the feedback pipe 40 is lower than that of the buffer bag lower liquid level sensor 34, the other end of the buffer bag 32 is connected with the whole blood communicating pipe 41, the feedback pipe 40 is clamped on the feedback peristaltic pump 25, one end of the anticoagulant pipe 42 is communicated with the anticoagulant bag 39, the other end of the anticoagulant pipe is communicated with the whole blood communicating pipe 41, the anticoagulant pipe 42 is clamped on the anticoagulant peristaltic pump 24, and the port of the whole blood communicating pipe 41 is provided with the puncture needle 43.

See fig. 4. The puncture needle 43 is punctured into a human body 44, the feedback peristaltic pump 25 rotates forwards at a flow rate larger than that of the anticoagulant peristaltic pump 24 to enable whole blood to enter the whole blood communicating pipe 41, the anticoagulant peristaltic pump 24 rotates forwards to enable anticoagulant in the anticoagulant bag 39 to enter the whole blood communicating pipe 41 and to be mixed with the whole blood to form anticoagulated whole blood, the anticoagulated whole blood enters the buffer bag 32 and reaches the buffer bag lower liquid level sensor 34, then the anticoagulant peristaltic pump 24 and the feedback peristaltic pump 25 are stopped rotating, and air in a pipeline can be safely evacuated to enable air not to enter the human body 44 during feedback.

See fig. 5. The tubular centrifuge 1 rotates along the centrifugal rotation central line 21, the first pinch valve 27 is opened, the second pinch valve 28 is closed, the third pinch valve 29 is closed, the fourth pinch valve 30 is opened, the fifth pinch valve 31 is closed, the anti-coagulated whole blood peristaltic pump 23 simultaneously rotates at a flow rate higher than that of the anti-coagulated whole blood peristaltic pump 24, so that the anti-coagulated whole blood enters the primary centrifugal area 2 from the anti-coagulated whole blood vessel 14, the anti-coagulated whole blood components are layered into red blood cells 45, white blood cells 46, platelets 47 and plasma 48 from far to near under the action of centrifugal force from the centrifugal rotation central line 21 due to different densities, the transfer peristaltic pump 26 positively rotates at a flow rate lower than that of the anti-coagulated whole blood peristaltic pump 23 to transfer the plasma 48 to the secondary centrifugal area 3 through the component blood vessel 15 and the transfer tube one 17 and flows into the communicating tube 32 through the transfer tube three 18 and the buffer bag 38, since the flow rate of the transfer peristaltic pump 26 is lower than the flow rate of the anti-coagulated whole blood peristaltic pump 23, the red blood cells 45 will continue to be expressed out of the red blood cell tube 16 into the buffer bag 32.

At this time, when the flow rate of the transfer peristaltic pump 26 is lower than the flow rate of the plasma separated from the anticoagulated whole blood entering the tube centrifuge 1, the total amount of the plasma 48 in the primary centrifugal region 2 tends to increase, all the cell layer interfaces in the primary centrifugal region 2 move in the direction away from the centrifugal rotation center line 21, and when the flow rate of the transfer peristaltic pump 26 is higher than the flow rate of the plasma separated from the anticoagulated whole blood entering the primary centrifugal region 2, the total amount of the plasma 48 in the primary centrifugal region 2 tends to decrease, and all the cell layer interfaces in the primary centrifugal region 2 move in the direction close to the centrifugal rotation center line 21.

Since the distance from each component blood in the tube centrifuge 1 to the centrifugal rotation center line 21 is much greater than the distance from each component blood to the untwisting rotation center line 22, the centrifugal rotation angular velocity is equal to the untwisting rotation angular velocity when the tube centrifuge 1 rotates along the centrifugal rotation center line 21, and the centrifugal acceleration applied to each component blood is much greater than the gravitational acceleration, it can be considered that the layering interface of each component blood is approximately parallel to the centrifugal rotation center line 21.

See fig. 6. When the liquid level in the buffer bag 32 reaches the upper buffer bag liquid level sensor 35, the feedback peristaltic pump 25 reversely rotates at a flow rate larger than that of the anti-coagulated whole blood peristaltic pump 23 to rapidly feed the blood component in the buffer bag 32 back to the human body 44 until the liquid level in the buffer bag 32 reaches the lower buffer bag liquid level sensor 34, and the feedback in the collection process is completed.

Preferably, the anti-coagulant whole blood peristaltic pump 23, the anti-coagulant peristaltic pump 24 and the transfer peristaltic pump 26 are decelerated in equal proportion when the reverse-flow peristaltic pump 25 is reversed.

See fig. 7. Keeping the tube centrifuge 1 rotating along the centrifugal rotation central line 21, opening the first tube clamp valve 27, closing the second tube clamp valve 28, closing the third tube clamp valve 29, opening the fourth tube clamp valve 30, closing the fifth tube clamp valve 31, increasing the flow rate of the transfer peristaltic pump 26 under the precondition that the flow rate of the transfer peristaltic pump 26 is lower than that of the anticoagulated whole blood peristaltic pump 23, moving the cell layer interface in the primary centrifugal area 2 to the direction close to the centrifugal rotation central line 21 until the blood component sensor 36 detects the white blood cells 46, transferring the platelets 47 from the primary centrifugal area 2 to the secondary centrifugal area 3, calculating the volume of the platelets 47 and the volume of the impurity cells transferred from the primary centrifugal area 2 to the secondary centrifugal area 3 in the process according to the time when the component sensor 36 detects the platelets 47 and the white blood cells 46 and the flow rate of the transfer peristaltic pump 26, and because the density of the blood components in the secondary centrifugal area 3 is different, layering from far to near the centrifugal rotation central line 21 into white blood due to the density difference of the blood components in the secondary centrifugal area 3 Cells 46, platelets 47, plasma 48.

This may also be accomplished by transferring platelets 47 from the primary centrifugation zone 2 to the secondary centrifugation zone 3 until the blood component sensor 36 detects red blood cells 45. Preferably, the volume of impurity cells entering the secondary centrifugation region 3 can be reduced by completing the transfer of platelets to the secondary centrifugation chamber upon detection of leukocytes 46 by the blood component sensor 36, and platelets with higher purity can be obtained.

See fig. 8. Keeping the tubular centrifuge 1 rotating along the centrifugal rotation central line 21, opening the first pinch valve 27, closing the second pinch valve 28, closing the third pinch valve 29, opening the fourth pinch valve 30, closing the fifth pinch valve 31, reducing the flow rate of the transfer peristaltic pump 26 under the precondition that the flow rate of the transfer peristaltic pump 26 is lower than that of the anticoagulated whole blood peristaltic pump 23, moving the cell stratification interface in the primary centrifugation area 2 away from the centrifugal rotation central line 21, at the moment, the plasma 48 of the component blood outlet 5 enters the secondary centrifugation area 3 from the primary centrifugation area 2 through the component blood vessel 15 and the transfer tube 17, flushing all the cells in the component blood vessel 15 and the transfer tube 17 to the secondary centrifugation area 3, preparing pure plasma 48 for collecting platelets 47 in the secondary centrifugation area 3 and removing impurity cells in the secondary centrifugation area 3 when the wheel collects the platelets 47, and continuously stratifies the whole blood in the primary centrifugation area 2, to prepare for the next round of collection, while prolonging the centrifugation time of the platelets 47 and leukocytes 46 in the secondary centrifugation zone 3 to allow more complete stratification.

See fig. 9. Keeping the tubular centrifuge 1 rotating along the centrifugal rotation central line 21, closing the first tube clamp valve 27, opening the second tube clamp valve 28, opening the third tube clamp valve 29, closing the fourth tube clamp valve 30, closing the fifth tube clamp valve 31, filling the plasma 48 in the primary centrifugal zone 2 into the secondary centrifugal zone 3 from the transfer port three 8 according to the volume of the impurity cells entering the secondary centrifugal zone 3 obtained in the process of transferring the platelets to the secondary centrifugal bin, and removing the impurity cells such as leukocytes 46 out of the secondary centrifugal zone 3 through the first transfer tube 17 and the impurity cell tube 37 and entering the anticoagulation whole blood vessel 14.

This process removes a portion of platelets 47 from the secondary centrifugation zone 3, which re-enters the primary centrifugation zone 2 from the anticoagulated whole blood vessel 14 to continue the next round of separation and collection, without leaving the tube centrifuge 1 and returning to the body, with little effect on platelet collection rate.

See fig. 10. Keeping the tube centrifuge 1 rotating along the centrifugal rotation central line 21, closing the first tube clamp valve 27, closing the second tube clamp valve 28, opening the third tube clamp valve 29, closing the fourth tube clamp valve 30, opening the fifth tube clamp valve 31, filling the plasma 48 in the primary centrifugal area 2 into the secondary centrifugal area 3 from the transfer port three 8 according to the volume of the platelets 47 entering the secondary centrifugal area 3 obtained in the process of transferring the platelets to the secondary centrifugal chamber, and allowing the platelets 47 to enter the platelet collection bag 33 through the transfer tube one 17.

See fig. 11. Circularly performing a process of transferring platelets to a secondary centrifugal chamber, a process of preparing the secondary centrifugal chamber to flush plasma, a process of removing impurity cells, and a process of collecting high-purity platelets until enough platelets are collected, keeping the tube centrifuge 1 to rotate along a centrifugal rotation central line 21, opening a first tube clamp valve 27, closing a second tube clamp valve 28, opening a third tube clamp valve 29, opening a fourth tube clamp valve 30, closing a fifth tube clamp valve 31, stopping the rotation of the anticoagulated whole blood peristaltic pump 23 and the anticoagulation peristaltic pump 24, reversing the transfer peristaltic pump 26 to enable air in the buffer bag 32 to be filled into the secondary centrifugal region 3 through the buffer bag communicating tube 38 and the transfer tube third 18, wherein the linear distance from blood components to the centrifugal rotation central line 21 is larger than air because the density of all blood components is larger than that of air, and the blood components in the secondary centrifugal region 3 are extruded to the primary centrifugal region 2 through the transfer tube first 17, then the red blood cells are extruded from the primary centrifugal area 2 to the buffer bag 32 through the red blood cell outlet 6 and are back-transfused to the human body 44 through the back-transfusing peristaltic pump 25, the blood components in the primary centrifugal area 2 and the secondary centrifugal area 3 can be emptied, and the collection and the back-transfusing process are completed.

The second specific embodiment:

see fig. 12. Preferably, since the possible residual impurity cells in the first transfer tube 17 in the "impurity cell removing process" of the first embodiment are collected in the platelet collection bag 33 in the "platelet collection process", in order to obtain a higher platelet purity than that in the first process, a platelet collection port 9 is additionally provided in the secondary centrifugation region 3 for collecting platelets exclusively to obtain a higher platelet purity. A high purity platelet continuous separation device comprising: be equipped with tubular centrifuge 1 of once centrifugal district 2 and secondary centrifugal district 3 and the elasticity of being connected with tubular centrifuge 1 and move back and turn round tube bank 10, its characterized in that: the primary centrifugal area 2 and the secondary centrifugal area 3 are isolated from each other, an anticoagulated whole blood vessel 14, a component blood vessel 15 and a erythrocyte tube 16 are fixed and inserted into the primary centrifugal area 2 of the tubular centrifuge 1, the anticoagulated whole blood vessel 14 is provided with an anticoagulated whole blood inlet 4 in the primary centrifugal area 2, the component blood vessel 15 is provided with a component blood outlet 5 in the primary centrifugal area 2, the erythrocyte tube 16 is provided with an erythrocyte outlet 6 in the primary centrifugal area 2, the anticoagulated whole blood inlet 4, the component blood outlet 5 and the erythrocyte outlet 6 are all positioned at the same side of a centrifugal rotation central line 21 in the centrifugal rotation process, and the vertical distances from the centrifugal rotation central line 21 are the erythrocyte outlet 6, the anticoagulated whole blood inlet 4 and the component blood outlet 5 from large to small in turn, the erythrocyte outlet 6 is close to the tube bottom 50 of the primary centrifugal area, a transfer tube I17, a transfer tube III 18 and a platelet collection tube 19 are fixed and inserted into the secondary centrifugal area 3 of the tubular centrifuge 1, the first transfer tube 17 is provided with a first transfer port 7 in the secondary centrifugal area 3, the third transfer tube 18 is provided with a third transfer port 8 in the secondary centrifugal area 3, the platelet collecting tube 19 is provided with a platelet collecting port 9 in the secondary centrifugal area 3, the first transfer port 7, the third transfer port 8 and the platelet collecting port 9 are all positioned at the same side of the centrifugal rotation central line 21 in the centrifugal rotation process, the first transfer port 7 and the platelet collecting port 9 are both close to the tube bottom 51 of the secondary centrifugal area, the vertical distances from the first transfer port 7 and the platelet collecting port 9 to the centrifugal rotation central line 21 are both greater than the vertical distance from the third transfer port 8 to the centrifugal rotation central line 21, the anticoagulation whole blood vessel 14 and the component blood vessel 15, the erythrocyte tube 16, the transfer tube I17, the transfer tube III 18 and the platelet collecting tube 19 are converged into an elastic untwisted tube bundle 10 at the same side outside the tubular centrifuge 1, and the elastic untwisted tube bundle 10 is provided with an elastic untwisted tube bundle fixing head 11.

See fig. 13. An anticoagulated whole blood vessel 14 is clamped on an anticoagulated whole blood peristaltic pump 23 and connected with a whole blood communicating pipe 41, a red cell pipe 16 is connected with a buffer bag 32, the horizontal position of a connecting port is not lower than that of a liquid level sensor 35 on the buffer bag, a component blood pipe 15 is clamped on a transfer peristaltic pump 26 and provided with a blood component sensor 36, the component blood pipe 15 is communicated with a transfer pipe I17 and provided with a pipe clamp valve I27 at the communicating position, the component blood pipe 15 is communicated with a transfer pipe III 18 and provided with a pipe clamp valve III 29 at the communicating position, the transfer pipe I17 is communicated with an impurity cell pipe 37 and provided with a pipe clamp valve II 28 at the communicating position, the transfer pipe III 18 is communicated with a buffer bag communicating pipe 38 and provided with a pipe clamp valve IV 30 at the communicating position, a platelet collecting pipe 19 is communicated with a platelet collecting bag 33 and provided with a pipe clamp valve V31, each pipe clamp valve is a pipeline which is not communicated with two ends of the platelet collecting bag when the platelet collecting bag is closed, the tube communicated with two ends of the impurity cell pipe when the platelet collecting bag is opened, the impurity cell pipe 37 is communicated with the anticoagulated whole blood vessel 14, the buffer bag communicating pipe 38 is communicated with the buffer bag 32, the horizontal position of the connector is not lower than that of the buffer bag upper liquid level sensor 35, the buffer bag 32 is provided with the buffer bag lower liquid level sensor 34 and the buffer bag upper liquid level sensor 35, one end of the feedback pipe 40 is lower than that of the buffer bag lower liquid level sensor 34, the other end of the buffer bag 32 is connected with the whole blood communicating pipe 41, the feedback pipe 40 is clamped on the feedback peristaltic pump 25, one end of the anticoagulant pipe 42 is communicated with the anticoagulant bag 39, the other end of the anticoagulant pipe is communicated with the whole blood communicating pipe 41, the anticoagulant pipe 42 is clamped on the anticoagulant peristaltic pump 24, and the port of the whole blood communicating pipe 41 is provided with the puncture needle 43.

See fig. 14. The puncture needle 43 is punctured into a human body 44, the feedback peristaltic pump 25 rotates forwards at a flow rate larger than that of the anticoagulant peristaltic pump 24 to enable whole blood to enter the whole blood communicating pipe 41, the anticoagulant peristaltic pump 24 rotates forwards to enable anticoagulant in the anticoagulant bag 39 to enter the whole blood communicating pipe 41 and to be mixed with the whole blood to form anticoagulated whole blood, the anticoagulated whole blood enters the buffer bag 32 and reaches the buffer bag lower liquid level sensor 34, then the anticoagulant peristaltic pump 24 and the feedback peristaltic pump 25 are stopped rotating, and air in a pipeline can be safely evacuated to enable air not to enter the human body 44 during feedback.

See fig. 15. The tubular centrifuge 1 rotates along the centrifugal rotation central line 21, the first pinch valve 27 is opened, the second pinch valve 28 is closed, the third pinch valve 29 is closed, the fourth pinch valve 30 is opened, the fifth pinch valve 31 is closed, the anti-coagulated whole blood peristaltic pump 23 simultaneously rotates at a flow rate higher than that of the anti-coagulated whole blood peristaltic pump 24, so that the anti-coagulated whole blood enters the primary centrifugal area 2 from the anti-coagulated whole blood vessel 14, the anti-coagulated whole blood components are layered into red blood cells 45, white blood cells 46, platelets 47 and plasma 48 from far to near under the action of centrifugal force from the centrifugal rotation central line 21 due to different densities, the transfer peristaltic pump 26 positively rotates at a flow rate lower than that of the anti-coagulated whole blood peristaltic pump 23 to transfer the plasma 48 to the secondary centrifugal area 3 through the component blood vessel 15 and the transfer tube one 17 and flows into the communicating tube 32 through the transfer tube three 18 and the buffer bag 38, since the flow rate of the transfer peristaltic pump 26 is lower than the flow rate of the anti-coagulated whole blood peristaltic pump 23, the red blood cells 45 will continue to be expressed out of the red blood cell tube 16 into the buffer bag 32.

See fig. 16. When the liquid level in the buffer bag 32 reaches the upper buffer bag liquid level sensor 35, the feedback peristaltic pump 25 reversely rotates at a flow rate larger than that of the anti-coagulated whole blood peristaltic pump 23 to rapidly feed the blood component in the buffer bag 32 back to the human body 44 until the liquid level in the buffer bag 32 reaches the lower buffer bag liquid level sensor 34, and the feedback in the collection process is completed.

See fig. 17. Keeping the tube centrifuge 1 rotating along the centrifugal rotation central line 21, opening the first tube clamp valve 27, closing the second tube clamp valve 28, closing the third tube clamp valve 29, opening the fourth tube clamp valve 30, closing the fifth tube clamp valve 31, increasing the flow rate of the transfer peristaltic pump 26 under the precondition that the flow rate of the transfer peristaltic pump 26 is lower than that of the anticoagulated whole blood peristaltic pump 23, moving the cell layer interface in the primary centrifugal area 2 to the direction close to the centrifugal rotation central line 21 until the blood component sensor 36 detects the white blood cells 46, transferring the platelets 47 from the primary centrifugal area 2 to the secondary centrifugal area 3, calculating the volume of the platelets 47 and the volume of the impurity cells transferred from the primary centrifugal area 2 to the secondary centrifugal area 3 in the process according to the time when the component sensor 36 detects the platelets 47 and the white blood cells 46 and the flow rate of the transfer peristaltic pump 26, and because the density of the blood components in the secondary centrifugal area 3 is different, layering from far to near the centrifugal rotation central line 21 into white blood due to the density difference of the blood components in the secondary centrifugal area 3 Cells 46, platelets 47, plasma 48.

See fig. 18. Keeping the tube centrifuge 1 rotating along the centrifugal rotation central line 21, opening the first tube clamp valve 27, closing the second tube clamp valve 28, closing the third tube clamp valve 29, opening the fourth tube clamp valve 30, closing the fifth tube clamp valve 31, reducing the flow rate of the transfer peristaltic pump 26 under the precondition that the flow rate of the transfer peristaltic pump 26 is lower than that of the anticoagulated whole blood peristaltic pump 23, so that the cell stratification interface in the primary centrifugation area 2 moves away from the centrifugal rotation central line 21, at the moment, the plasma 48 of the component blood outlet 5 enters the secondary centrifugation area 3 from the primary centrifugation area 2 through the component blood vessel 15 and the first transfer tube 17, and all the cells in the component blood vessel 15 and the first transfer tube 17 are washed to the secondary centrifugation area 3, and simultaneously prolonging the centrifugation time of the platelets 47 and the white blood cells 46 in the secondary centrifugation area 3, so that the cells are more thoroughly layered.

See fig. 19. Keeping the tubular centrifuge 1 rotating along the centrifugal rotation central line 21, closing the first tube clamp valve 27, opening the second tube clamp valve 28, opening the third tube clamp valve 29, closing the fourth tube clamp valve 30, closing the fifth tube clamp valve 31, filling the plasma 48 in the primary centrifugal zone 2 into the secondary centrifugal zone 3 from the transfer port three 8 according to the volume of the impurity cells entering the secondary centrifugal zone 3 obtained in the process of transferring the platelets into the secondary centrifugal bin, and moving the impurity cells such as the white blood cells 46 out of the secondary centrifugal zone 3 through the first transfer tube 17 and the impurity cell tube 37 and entering the anticoagulation whole blood vessel 14, wherein in the process, part of the platelets 47 may be removed from the secondary centrifugal zone 3, and enter the primary centrifugal zone 2 again from the anticoagulation whole blood vessel 14 to continue the next round of separation and collection, so that the platelets cannot leave the system and are returned to the human body, and the platelet collection rate is ensured.

See fig. 20. Keeping the tube centrifuge 1 rotating along the centrifugal rotation central line 21, closing the first tube clamp valve 27, closing the second tube clamp valve 28, opening the third tube clamp valve 29, closing the fourth tube clamp valve 30, opening the fifth tube clamp valve 31, filling the plasma 48 in the primary centrifugal area 2 from the transfer port three 8 into the secondary centrifugal area 3 according to the volume of the platelets 47 entering the secondary centrifugal area 3 obtained in the process of transferring the platelets to the secondary centrifugal chamber, and allowing the platelets 47 to enter the platelet collection bag 33 through the platelet collection tube 19.

See fig. 21. Circularly performing a process of transferring platelets to a secondary centrifugal chamber, a process of preparing the secondary centrifugal chamber to flush plasma, a process of removing impurity cells, and a process of collecting high-purity platelets until enough platelets are collected, keeping the tube centrifuge 1 to rotate along a centrifugal rotation central line 21, opening a first tube clamp valve 27, closing a second tube clamp valve 28, opening a third tube clamp valve 29, opening a fourth tube clamp valve 30, closing a fifth tube clamp valve 31, stopping the rotation of the anticoagulated whole blood peristaltic pump 23 and the anticoagulation peristaltic pump 24, reversing the transfer peristaltic pump 26 to enable air in the buffer bag 32 to be filled into the secondary centrifugal region 3 through the buffer bag communicating tube 38 and the transfer tube third 18, wherein the linear distance from blood components to the centrifugal rotation central line 21 is larger than air because the density of all blood components is larger than that of air, and the blood components in the secondary centrifugal region 3 are extruded to the primary centrifugal region 2 through the transfer tube first 17, then the red blood cells are extruded from the primary centrifugal area 2 to the buffer bag 32 through the red blood cell outlet 6 and are back-transfused to the human body 44 through the back-transfusing peristaltic pump 25, the blood components in the primary centrifugal area 2 and the secondary centrifugal area 3 can be emptied, and the collection and the back-transfusing process are completed.

The third concrete implementation scheme is as follows:

see fig. 22. In order to simplify the design of an external pipeline, reduce the number of external valves simply and reduce the use of a three-way pipe and a multi-way pipe so as to avoid damping a blood component passage, a second transfer pipe 17-1 can be additionally arranged in a secondary centrifugal area 3, and the high-purity platelet continuous separation device comprises: be equipped with tubular centrifuge 1 of once centrifugal district 2 and secondary centrifugal district 3 and the elasticity of being connected with tubular centrifuge 1 and move back and turn round tube bank 10, its characterized in that: the primary centrifugal area 2 and the secondary centrifugal area 3 are isolated from each other, an anticoagulated whole blood vessel 14, a component blood vessel 15 and a erythrocyte tube 16 are fixed and inserted into the primary centrifugal area 2 of the tubular centrifuge 1, the anticoagulated whole blood vessel 14 is provided with an anticoagulated whole blood inlet 4 in the primary centrifugal area 2, the component blood vessel 15 is provided with a component blood outlet 5 in the primary centrifugal area 2, the erythrocyte tube 16 is provided with an erythrocyte outlet 6 in the primary centrifugal area 2, the anticoagulated whole blood inlet 4, the component blood outlet 5 and the erythrocyte outlet 6 are all positioned at the same side of a centrifugal rotation central line 21 in the centrifugal rotation process, the vertical distances from the centrifugal rotation central line 21 are the erythrocyte outlet 6, the anticoagulated whole blood inlet 4 and the component blood outlet 5 from large to small in turn, the erythrocyte outlet 6 is close to the tube bottom 50 of the primary centrifugal area, a transfer tube I17, a transfer tube II 17-1, a transfer tube III 18 and a platelet collection tube 19 are fixed and inserted into the secondary centrifugal area 3 of the tubular centrifuge 1, the first transfer tube 17 is provided with a first transfer port 7 in the secondary centrifugal area 3, the second transfer tube 17-1 is provided with a second transfer port 7-1 in the secondary centrifugal area 3, the third transfer tube 18 is provided with a third transfer port 8 in the secondary centrifugal area 3, the platelet collecting tube 19 is provided with a platelet collecting port 9 in the secondary centrifugal area 3, the first transfer port 7, the second transfer port 7-1, the third transfer port 8 and the platelet collecting port 9 are all positioned at the same side of the centrifugal rotation central line 21 in the centrifugal rotation process, the first transfer port 7, the second transfer port 7-1 and the platelet collecting port 9 are all close to the tube bottom 51 of the secondary centrifugal area, the vertical distances from the first transfer port 7, the second transfer port 7-1 and the platelet collecting port 9 to the centrifugal rotation central line 21 are all larger than the vertical distance from the third transfer port 8 to the centrifugal rotation central line 21, the anticoagulated whole blood vessel 14 and the component blood vessel 15, The erythrocyte tube 16, the first transfer tube 17, the second transfer tube 17-1, the third transfer tube 18 and the platelet collecting tube 19 are converged into an elastic untwisted tube bundle 10 at the same side outside the tubular centrifuge 1, and the elastic untwisted tube bundle 10 is provided with an elastic untwisted tube bundle fixing head 11.

See fig. 23. An anticoagulated whole blood vessel 14 is clamped on an anticoagulated whole blood peristaltic pump 23 and connected with a whole blood communicating pipe 41, a red cell tube 16 is connected with a buffer bag 32, the horizontal position of a connecting port is not lower than the liquid level sensor 35 on the buffer bag, a component blood tube 15 is clamped on a transfer peristaltic pump 26 and provided with a blood component sensor 36, the component blood tube 15 is communicated with a transfer tube two 17-1, the transfer tube one 17 is communicated with an impurity cell tube 37, the communicating part of the transfer tube one 17 is provided with a tube clamp valve two 28, the transfer tube three 18 is communicated with the buffer bag communicating pipe 38, the communicating part of the transfer tube three is provided with a tube clamp valve four 30, a platelet collecting tube 19 is communicated with a platelet collecting bag 33 and provided with a tube clamp valve five 31, each tube clamp valve is a closed pipeline with two ends connected and communicated, the pipelines with two ends connected are communicated when the tube is opened, the impurity cell tube 37 is communicated with the anticoagulated whole blood vessel 14, the buffer bag 38 is communicated with the buffer bag 32, and the horizontal position of the connecting port is not lower than the liquid level sensor 35 on the buffer bag, the buffer bag 32 is provided with a buffer bag lower liquid level sensor 34 and a buffer bag upper liquid level sensor 35, one end of the feedback pipe 40 is lower than the buffer bag lower liquid level sensor 34, the other end of the buffer bag 32 is connected with a whole blood communicating pipe 41, the feedback pipe 40 is clamped on the feedback peristaltic pump 25, one end of the anticoagulant pipe 42 is communicated with the anticoagulant bag 39, the other end of the anticoagulant pipe is communicated with the whole blood communicating pipe 41, the anticoagulant pipe 42 is clamped on the anticoagulant peristaltic pump 24, and the port of the whole blood communicating pipe 41 is provided with a puncture needle 43.

See fig. 24. The puncture needle 43 is punctured into a human body 44, the feedback peristaltic pump 25 rotates forwards at a flow rate larger than that of the anticoagulant peristaltic pump 24 to enable whole blood to enter the whole blood communicating pipe 41, the anticoagulant peristaltic pump 24 rotates forwards to enable anticoagulant in the anticoagulant bag 39 to enter the whole blood communicating pipe 41 and to be mixed with the whole blood to form anticoagulated whole blood, the anticoagulated whole blood enters the buffer bag 32 and reaches the buffer bag lower liquid level sensor 34, then the anticoagulant peristaltic pump 24 and the feedback peristaltic pump 25 are stopped rotating, and air in a pipeline can be safely evacuated to enable air not to enter the human body 44 during feedback.

See fig. 25. The tubular centrifuge 1 rotates along the centrifugal rotation central line 21, the second pinch valve 28 is closed, the fourth pinch valve 30 is opened, the fifth pinch valve 31 is closed, the anti-coagulated whole blood peristaltic pump 23 rotates at the same time at a flow rate higher than that of the anti-coagulated whole blood peristaltic pump 24, so that the anti-coagulated whole blood enters the primary centrifugal area 2 from the anti-coagulated whole blood vessel 14, the anti-coagulated whole blood components are layered into red blood cells 45, white blood cells 46, platelets 47 and plasma 48 from far to near from the central rotation central line 21 under the action of centrifugal force due to different densities, the transfer peristaltic pump 26 positively rotates at a flow rate lower than that of the anti-coagulated whole blood peristaltic pump 23 to transfer the plasma 48 to the secondary centrifugal area 3 through the component blood vessel 15 and the transfer tube II 17-1 and flows into the buffer bag 32 through the transfer tube III 18 and the buffer bag communicating tube 38, since the flow rate of the transfer peristaltic pump 26 is lower than the flow rate of the anti-coagulated whole blood peristaltic pump 23, the red blood cells 45 will continue to be expressed out of the red blood cell tube 16 into the buffer bag 32.

See fig. 26. When the liquid level in the buffer bag 32 reaches the upper buffer bag liquid level sensor 35, the feedback peristaltic pump 25 reversely rotates at a flow rate larger than that of the anti-coagulated whole blood peristaltic pump 23 to rapidly feed the blood component in the buffer bag 32 back to the human body 44 until the liquid level in the buffer bag 32 reaches the lower buffer bag liquid level sensor 34, and the feedback in the collection process is completed.

See fig. 27. Keeping the tube centrifuge 1 rotating along the centrifugal rotation central line 21, closing the tube clamp valve II 28, opening the tube clamp valve IV 30, closing the tube clamp valve V31, increasing the flow rate of the transfer peristaltic pump 26 under the precondition that the flow rate of the transfer peristaltic pump 26 is lower than that of the anticoagulated whole blood peristaltic pump 23, moving the cell layer interface in the primary centrifugal area 2 to the direction close to the centrifugal rotation central line 21 until the blood component sensor 36 detects the white blood cells 46, transferring the platelets 47 from the primary centrifugal area 2 to the secondary centrifugal area 3, calculating the volume of the platelets 47 and the volume of the impurity cells transferred from the primary centrifugal area 2 to the secondary centrifugal area 3 in the process according to the time when the component sensor 36 detects the platelets 47 and the white blood cells 46 and the flow rate of the transfer peristaltic pump 26, and calculating the volume of the platelets 47 and the impurity cells transferred from the primary centrifugal area 2 to the secondary centrifugal area 3 from far to near to the centrifugal rotation central line 21 due to the difference of the density of the blood components in the secondary centrifugal area 3, Platelets 47, plasma 48.

See fig. 28. Keeping the tube centrifuge 1 rotating along the centrifugal rotation central line 21, closing the tube clamp valve II 28, opening the tube clamp valve IV 30, closing the tube clamp valve V31, reducing the flow rate of the transfer peristaltic pump 26 under the precondition that the flow rate of the transfer peristaltic pump 26 is lower than that of the anticoagulated whole blood peristaltic pump 23, moving the cell stratification interface in the primary centrifugation area 2 to the direction far away from the centrifugal rotation central line 21, at the moment, the plasma 48 of the component blood outlet 5 enters the secondary centrifugation area 3 from the primary centrifugation area 2 through the component blood vessel 15 and the transfer tube II 17-1, washing all the cells in the component blood vessel 15 and the transfer tube II 17-1 to the secondary centrifugation area 3, and prolonging the centrifugation time of the platelets 47 and the white blood cells 46 in the secondary centrifugation area 3, so that the cells are layered more thoroughly.

See fig. 29. Keeping the tubular centrifuge 1 rotating along the centrifugal rotation central line 21, opening the second tube clamp valve 28, closing the fourth tube clamp valve 30, closing the fifth tube clamp valve 31, filling the plasma 48 in the primary centrifugal area 2 from the second transfer tube 17-1 into the secondary centrifugal area 3 according to the volume of the impurity cells entering the secondary centrifugal area 3 obtained in the process of transferring the platelets into the secondary centrifugal chamber, and removing the impurity cells such as leucocytes 46 from the secondary centrifugal area 3 through the first transfer tube 17 and the impurity cell tube 37 and entering the anticoagulation whole blood vessel 14, wherein in the process, part of the platelets 47 may be removed from the secondary centrifugal area 3, and enter the primary centrifugal area 2 again from the anticoagulation whole blood vessel 14 to continue to be separated and collected for the next round without leaving the system and returning to the human body, thereby ensuring the platelet collection rate.

See fig. 30. Keeping the tube centrifuge 1 rotating along the centrifugal rotation central line 21, closing the second tube clamp valve 28, closing the fourth tube clamp valve 30, opening the fifth tube clamp valve 31, filling the plasma 48 in the primary centrifugal area 2 from the second transfer tube 17-1 into the secondary centrifugal area 3 according to the volume of the platelets 47 entering the secondary centrifugal area 3 obtained in the process of transferring the platelets to the secondary centrifugal chamber, and allowing the platelets 47 to enter the platelet collection bag 33 through the platelet collection tube 19.

See fig. 31. The process of 'platelet transfer to secondary centrifugal bin flow', 'preparation of secondary centrifugal bin plasma flushing flow', 'impurity cell removal flow', 'collection of high purity platelet flow' is carried out circularly until enough platelets are collected, the tubular centrifuge 1 is kept to rotate along the centrifugal rotation central line 21, the second pinch valve 28 is closed, the fourth pinch valve 30 is opened, the fifth pinch valve 31 is closed, the rotation of the anticoagulated whole blood peristaltic pump 23 and the anticoagulant peristaltic pump 24 is stopped, the transfer peristaltic pump 26 is reversed, so that the air in the buffer bag 32 is filled into the secondary centrifugal zone 3 through the buffer bag communicating pipe 38 and the third transfer pipe 18, the linear distance from the blood components to the centrifugal rotation central line 21 is larger than that of air due to the fact that the density of all the blood components is larger than that of air, the blood components in the secondary centrifugal zone 3 are extruded to the primary centrifugal zone 2 through the second transfer pipe 17-1, and then are extruded to the buffer bag 32 from the primary centrifugal zone 2 through the red cell outlet 6 and are returned to the human body 44 through the return peristaltic pump 25, the blood components in the primary centrifugal area 2 and the secondary centrifugal area 3 can be emptied, and the collection and the return transfusion process are completed.

If the double-needle continuous platelet collection is to be realized, the return tube 40 can be connected with another puncture needle and can be punctured to another part of the human body.

The method for detecting the liquid level in the buffer bag 32 can be realized by adopting a weighing mode besides the mode of adopting the lower buffer bag liquid level sensor 34 and the upper buffer bag liquid level sensor 35.

If plasma is collected during platelet collection, a plasma collecting tube, a plasma collecting bag and a corresponding tube clamp valve can be additionally arranged at any position on a plasma flowing passage.

The first pinch valve 27 and the second pinch valve 28 may be combined into a gate pinch valve, and the third pinch valve 29 and the fourth pinch valve 30 may be combined into a gate pinch valve.

The foregoing is directed to the preferred embodiment of the present invention and it is noted that the above-mentioned embodiments illustrate rather than limit the invention, and that those skilled in the art will be able to design alternative embodiments without departing from the scope of the appended claims. Various other modifications and changes may be made by those skilled in the art based on the above-described technical solutions and concepts, and all such modifications and changes should fall within the scope of the claims of the present invention.

Claims (10)

1. A high purity platelet continuous separation device comprising: be equipped with tubular centrifuge (1) in once centrifugal district (2) and secondary centrifugal district (3) and the elasticity back twist tube bank (10) of being connected with tubular centrifuge (1), its characterized in that: the primary centrifugal area (2) and the secondary centrifugal area (3) are mutually isolated, an anticoagulated whole blood vessel (14), a component blood vessel (15) and a erythrocyte tube (16) are fixed and inserted into the primary centrifugal area (2) of the tubular centrifuge (1), the anticoagulated whole blood vessel (14) is provided with an anticoagulated whole blood inlet (4) in the primary centrifugal area (2), the component blood vessel (15) is provided with a component blood outlet (5) in the primary centrifugal area (2), the erythrocyte tube (16) is provided with an erythrocyte outlet (6) in the primary centrifugal area (2), the anticoagulated whole blood inlet (4), the component blood outlet (5) and the erythrocyte outlet (6) are all positioned on a centrifugal rotation central line (21) in the centrifugal rotation process, and the vertical distances to the centrifugal rotation central line (21) are sequentially the erythrocyte outlet (6), the anticoagulated whole blood inlet (4) and the component blood outlet (5) from the same side to the same side, the erythrocyte outlet (6) is close to the tube bottom (50) of the primary centrifugal area, the secondary centrifugal area (3) is at least provided with a first transfer tube (17) and a third transfer tube (18), the first transfer tube (17) and the third transfer tube (18) are fixed and inserted into the secondary centrifugal area (3) of the tube centrifuge (1), the first transfer tube (17) is provided with a first transfer port (7) in the secondary centrifugal area (3), the third transfer tube (18) is provided with a third transfer port (8) in the secondary centrifugal area (3), the first transfer port (7) and the third transfer port (8) are both positioned on the same side of the centrifugal rotation central line (21) in the centrifugal rotation process, the first transfer port (7) is close to the tube bottom (51) of the secondary centrifugal area, the vertical distance from the first transfer port (7) to the centrifugal rotation central line (21) is greater than the vertical distance from the third transfer port (8) to the centrifugal rotation central line (21), and the anticoagulation whole blood vessel (14), The component blood vessel (15), the erythrocyte tube (16), the transfer tube I (17) and the transfer tube III (18) are converged into an elastic untwisted tube bundle (10) at the same side of the outer part of the tubular centrifuge (1), and the elastic untwisted tube bundle (10) is provided with an elastic untwisted tube bundle fixing head (11).

2. A high purity platelet continuous separation apparatus according to claim 1, wherein: the secondary centrifugal area (3) can be additionally provided with a platelet collecting tube (19) and a second transfer tube (17-1), and a platelet collecting port (9) of the platelet collecting tube (19) in the secondary centrifugal area (3) and a second transfer port (7-1) of the second transfer tube (17-1) in the secondary centrifugal area (3) are both close to the tube bottom (51) of the secondary centrifugal area.

3. A high purity platelet continuous separation apparatus according to claim 1, wherein: the centrifugal disc motor (13) is connected with the centrifugal disc (20), a centrifuge back-twist support (12) fixed on the outer ring of a bearing is arranged on the centrifugal disc (20), the centrifugal disc motor (13) is fixed on a rack (49), an elastic back-twist tube bundle fixing head (11) on an elastic back-twist tube bundle (10) is fixed on the rack (49), and the tubular centrifuge (1) is inserted into the centrifuge back-twist support (12) with the bearing and is tightly matched with the inner ring of the bearing; when the centrifugal disc motor (13) rotates, the centrifuge untwisting bracket (12) on the centrifugal disc (20) drives the tubular centrifuge (1) to rotate along the centrifugal rotation central line (21), and simultaneously, the elastic untwisting tube bundle (10) has elastic stress, so that the tubular centrifuge (1) is driven to rotate and untwist along the untwisting rotation central line (22).

4. A high purity platelet continuous separation apparatus according to claim 1, wherein: keeping the tubular centrifuge (1) rotating along the centrifugal rotation central line (21), the anticoagulated whole blood enters the primary centrifugal area (2) from the anticoagulated whole blood vessel (14), the anticoagulated whole blood component is layered into red blood cells (45), white blood cells (46), platelets (47) and plasma (48) from far to near under the action of centrifugal force under the action of different densities from the centrifugal rotation central line (21), the transfer peristaltic pump (26) positively rotates at a flow rate lower than that of the anticoagulated whole blood peristaltic pump (23) to transfer the component blood of the primary centrifugal area (2) from the component blood outlet (5) to the secondary centrifugal area (3), the plasma (48) flows out from the transfer port three (8) of the secondary centrifugal area (3) and flows into the buffer bag (32), the red blood cells (45) are continuously extruded from the erythrocyte tube (16) to the buffer bag (32), under the condition that the anticoagulated whole blood flow rate flowing into the primary centrifugal area (2) is higher than the component blood flow rate flowing out of the primary centrifugal area (2), when the flow rate of the component blood flowing out of the primary centrifugal area (2) is lower than the flow rate of the plasma separated from the anticoagulated whole blood flowing into the primary centrifugal area (2), all cell layered interfaces in the primary centrifugal area (2) move towards the direction far away from the centrifugal rotation center line (21), and when the flow rate of the component blood flowing out of the tubular centrifuge (1) is higher than the flow rate of the plasma separated from the anticoagulated whole blood flowing into the primary centrifugal area (2), all cell layered interfaces in the primary centrifugal area (2) move towards the direction close to the centrifugal rotation center line (21).

5. A high purity platelet continuous separation apparatus according to claim 1, wherein: keeping the tubular centrifuge (1) rotating along the centrifugal rotation center line (21), increasing the flow rate of the component blood flowing out of the primary centrifugal area (2) under the precondition that the flow rate of the anticoagulated whole blood flowing into the primary centrifugal area (2) is ensured to be larger than the flow rate of the component blood flowing out of the primary centrifugal area (2), enabling the cell stratification interface in the primary centrifugal area (2) to move towards the direction close to the centrifugal rotation center line (21) until the blood component sensor (36) detects the white blood cells (46), and transferring the platelets (47) from the primary centrifugal area (2) to the secondary centrifugal area (3).

6. A high purity platelet continuous separation apparatus according to claim 1, wherein: keeping the tube type centrifuge (1) to rotate along the centrifugal rotation central line (21), injecting plasma into the secondary centrifugal area (3) from any pipe orifice of the secondary centrifugal area (3), and leading white blood cells (46) and other impurity cells to flow out of the secondary centrifugal area (3) from a pipe orifice close to the pipe bottom (51) of the secondary centrifugal area before platelets (47).

7. A high purity platelet continuous separation apparatus according to claim 1, wherein: and removing impurity cells such as white blood cells (46) from the secondary centrifugal area (3), and then returning the removed part of platelets (47) from the anticoagulation whole blood vessel (14) to the primary centrifugal area (2) for further separation and collection.

8. A high purity platelet continuous separation apparatus according to claim 1, wherein: keeping the tube centrifuge (1) rotating along the centrifugal rotation central line (21), when the impurity cells such as leucocytes (46) and the like flow out of the secondary centrifugal area from the pipe orifice close to the tube bottom (51) of the secondary centrifugal area, continuously filling the plasma into the secondary centrifugal area (3) from any pipe orifice of the secondary centrifugal area (3), and enabling the pure platelets (47) to flow out of the secondary centrifugal area (3) from the pipe orifice close to the tube bottom (51) of the secondary centrifugal area.

9. A high purity platelet continuous separation apparatus according to claim 1, wherein: keep tubular centrifuge (1) rotatory along centrifugation rotation center line (21), stop anticoagulation whole blood peristaltic pump (23) and anticoagulant peristaltic pump (24) rotatory, pour into secondary centrifugation district (3) by arbitrary transfer port with the air for the composition blood of secondary centrifugation district (3) flows into primary centrifugation district (2) from pressing close to secondary centrifugation district socle (51) and the mouth of pipe that communicates with primary centrifugation district (2), continue to pour into the air and make the composition blood of primary centrifugation district (2) extrude buffer bag (32) by erythrocyte export (6), reinfusion peristaltic pump (25) reinfusion to human body (44).

10. A high purity platelet continuous separation apparatus according to claim 1, wherein: the primary centrifugal area (2) and the secondary centrifugal area (3) can realize the component blood transfer process and the air transfer process by arranging peristaltic pumps on the component blood vessels (15) or can also realize the component blood transfer process by arranging the peristaltic pumps on the transfer tube I (17), the transfer tube II (17-1), the transfer tube III (18) and the platelet collection tube (19) respectively.

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