CN214361426U - 电穿孔盒筒和电穿孔系统 - Google Patents

电穿孔盒筒和电穿孔系统 Download PDF

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Publication number
CN214361426U
CN214361426U CN202022401077.3U CN202022401077U CN214361426U CN 214361426 U CN214361426 U CN 214361426U CN 202022401077 U CN202022401077 U CN 202022401077U CN 214361426 U CN214361426 U CN 214361426U
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electroporation
chamber
cartridge
electrode
volume
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Chinese (zh)
Inventor
S·H·约
S·W·洪
魏寒
Y·佩克
W·X·李
B·石
C·W·陈
B·H·林
D·山穆加姆
S·张
J·Y·谭
K·S·钟
M·高登
N·安德罗尼库
C·洛斯曼
X·德莫莱拉迪热
U·福克
L·Y·林
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Life Technology Holdings Pte Ltd
Life Technologies Corp
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Life Technology Holdings Pte Ltd
Life Technologies Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/44—Multiple separable units; Modules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Electromagnetism (AREA)
  • Cell Biology (AREA)
  • Thermal Sciences (AREA)
  • Computer Hardware Design (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
CN202022401077.3U 2019-10-25 2020-10-26 电穿孔盒筒和电穿孔系统 Active CN214361426U (zh)

Applications Claiming Priority (2)

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US201962926078P 2019-10-25 2019-10-25
US62/926,078 2019-10-25

Publications (1)

Publication Number Publication Date
CN214361426U true CN214361426U (zh) 2021-10-08

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CN202011154566.1A Pending CN112708552A (zh) 2019-10-25 2020-10-26 用于含细胞流体的电穿孔的系统、方法和设备

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US (2) US20210123009A1 (https=)
EP (1) EP4048776A1 (https=)
JP (2) JP7784374B2 (https=)
KR (1) KR20220088469A (https=)
CN (2) CN214361426U (https=)
AU (1) AU2020370481B2 (https=)
BR (1) BR112022007622A2 (https=)
CA (1) CA3154506A1 (https=)
DE (1) DE202020106067U1 (https=)
WO (1) WO2021081381A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112708552A (zh) * 2019-10-25 2021-04-27 生命科技控股私人有限公司 用于含细胞流体的电穿孔的系统、方法和设备

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12097345B2 (en) * 2018-06-01 2024-09-24 Penumbra, Inc. Infusion catheter and methods of use
USD943760S1 (en) * 2020-05-11 2022-02-15 Maxcyte, Inc. Electroporation processing assembly
USD944410S1 (en) 2020-05-11 2022-02-22 Maxcyte, Inc. Electroporation processing assembly
USD965170S1 (en) * 2020-10-23 2022-09-27 Life Technologies Corporation Electroporation device
US20230110090A1 (en) * 2021-10-08 2023-04-13 Life Technologies Holdings Pte Limited System and Method of Electroporation Protocol Optimization
TW202400835A (zh) 2022-03-09 2024-01-01 荷蘭商Ａｓｍ Ｉｐ私人控股有限公司 反應器系統、控制液體源的溫度之方法、及經調適以容納容器的裝置
WO2023230253A1 (en) * 2022-05-27 2023-11-30 Sqz Biotechnologies Company Cartridges and devices for use in a system for delivery of a payload into a cell
CN117431158A (zh) * 2022-07-14 2024-01-23 苏州壹达生物科技有限公司 一种流式电穿孔装置
US20260092248A1 (en) 2022-09-15 2026-04-02 Life Technologies Corporation Bioreactors operable in static and dynamic modes and methods of use
KR102609307B1 (ko) * 2022-10-24 2023-12-04 티큐브잇(주) 유전자 형질 감염 장치
CN115722292B (zh) * 2022-11-22 2023-12-29 山东省煤田地质局第一勘探队 一种地下水自然资源调查用分类收集装置及使用方法
CN120882852A (zh) * 2023-06-21 2025-10-31 隆萨科隆有限公司 多腔室电穿孔盒及其使用方法
WO2026043806A2 (en) 2024-08-19 2026-02-26 Life Technologies Corporation Systems, methods, and assemblies for cell processing
CN120028438B (zh) * 2025-04-23 2025-08-26 晋城市鑫环球铸造有限公司 一种基于球墨铸铁管生产的探伤检测设备及方法

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5545130A (en) * 1992-04-08 1996-08-13 Genetronics, Inc. Flow through electroporation method
US5650305A (en) * 1996-05-15 1997-07-22 Health Research Inc. Method to produce hybrid cells by electrofusion
US6150148A (en) 1998-10-21 2000-11-21 Genetronics, Inc. Electroporation apparatus for control of temperature during the process
US6969604B1 (en) * 2003-06-20 2005-11-29 Yakovenko Sergey A Electroporation chamber
US20050282283A1 (en) * 2004-04-19 2005-12-22 Laura Vozza-Brown Electroporation apparatus and methods
DK1766057T3 (en) * 2004-05-12 2015-03-02 Maxcyte Inc Methods and devices related to a regulated flowelektroporationskammer
WO2006112870A1 (en) * 2005-04-19 2006-10-26 Excellin Life Sciences, Inc. Device and method for controlled electroporation and molecular delivery in cells and tissue
WO2011050009A1 (en) * 2009-10-19 2011-04-28 Rational Biotechnology Inc. Method, device and apparatus for inducing self-adjusting cell electroporation
US9382510B2 (en) 2011-08-25 2016-07-05 Jian Chen Methods and devices for electroporation
US9204833B2 (en) * 2012-03-27 2015-12-08 Phlebotics, Inc. Cartridge for automated blood sampling system
US8766641B2 (en) * 2012-08-28 2014-07-01 Halliburton Energy Services, Inc. Determining surface wetting of rock with changing well fluids
CN104403943B (zh) * 2014-12-02 2016-06-15 陈剑 一种电击管和具有电击管的细胞电转仪
WO2016088062A1 (zh) * 2014-12-02 2016-06-09 陈剑 一种电击管和具有电击管的细胞电转仪
CA3017108A1 (en) * 2015-03-12 2016-09-15 The Trustees Of The University Of Pennsylvania System, method, and device for high-throughput, automated culturing of genetically modified organisms
JP6660057B2 (ja) 2015-12-11 2020-03-04 国立大学法人豊橋技術科学大学 エレクトロポレーション用電極及び外来物質導入装置
MX2019015505A (es) * 2017-06-30 2020-07-28 Inscripta Inc Metodos, modulos, instrumentos y sistemas de procesamiento de celulas automatizados.
CN109679844B (zh) 2017-10-19 2023-08-22 苏州壹达生物科技有限公司 一种流式电穿孔装置
JP2021506309A (ja) * 2017-12-20 2021-02-22 スクイーズ バイオテクノロジーズ カンパニー 細胞の中へのペイロードの送達のためのシステム
US20190225928A1 (en) * 2018-01-22 2019-07-25 Inscripta, Inc. Automated cell processing methods, modules, instruments, and systems comprising filtration devices
EP4048776A1 (en) * 2019-10-25 2022-08-31 Life Technologies Corporation Systems and devices for electroporation of cell-containing fluid

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112708552A (zh) * 2019-10-25 2021-04-27 生命科技控股私人有限公司 用于含细胞流体的电穿孔的系统、方法和设备

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AU2020370481A1 (en) 2022-05-26
WO2021081381A1 (en) 2021-04-29
JP2026040486A (ja) 2026-03-09
CA3154506A1 (en) 2021-04-29
US20210123009A1 (en) 2021-04-29
EP4048776A1 (en) 2022-08-31
AU2020370481B2 (en) 2026-04-02
KR20220088469A (ko) 2022-06-27
JP2023501142A (ja) 2023-01-18
DE202020106067U1 (de) 2020-12-04
JP7784374B2 (ja) 2025-12-11
BR112022007622A2 (pt) 2022-10-11
US20240263124A1 (en) 2024-08-08
CN112708552A (zh) 2021-04-27

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