CN213739524U - Cell culture bottle difficult to pollute and easy to identify - Google Patents

Cell culture bottle difficult to pollute and easy to identify Download PDF

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Publication number
CN213739524U
CN213739524U CN202022383294.4U CN202022383294U CN213739524U CN 213739524 U CN213739524 U CN 213739524U CN 202022383294 U CN202022383294 U CN 202022383294U CN 213739524 U CN213739524 U CN 213739524U
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CN
China
Prior art keywords
groove
culture bottle
easy
bottle body
wall
Prior art date
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Expired - Fee Related
Application number
CN202022383294.4U
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Chinese (zh)
Inventor
吴迪
尹元琴
李妍
张玲
姜又红
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
First Hospital of China Medical University
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First Hospital of China Medical University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
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Priority to CN202022383294.4U priority Critical patent/CN213739524U/en
Application granted granted Critical
Publication of CN213739524U publication Critical patent/CN213739524U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

The utility model discloses a difficult cell culture bottle who pollutes easy discernment, including the blake bottle body, set up in the culture solution input port of blake bottle body one side and set up in the scavenge port on blake bottle body top, the inside filter screen that is provided with of scavenge port, the scavenge port top is rotated and is provided with and is used for coveing the closing cap of filter screen, closing cap one end with be provided with the bullet between scavenge port one side and detain the subassembly, be provided with the valve plug in the culture solution input port inner wall. The utility model discloses a valve plug lift operation can shield the intraoral hole of culture solution input and close, leads to outside dust to get into when preventing to this internal nutrient solution of carrying of blake bottle, leads to the cell death of cultivateing, rotates through the closing cap simultaneously and opens and can take a breath to this internal portion of blake bottle, makes the inside cell of blake bottle carry out aerobic respiration, increases activity to turn round the lid through the split and can make and wash this internal wall of blake bottle more convenient.

Description

Cell culture bottle difficult to pollute and easy to identify
Technical Field
The utility model relates to a cell culture bottle technical field specifically is a difficult cell culture bottle who pollutes easy discernment.
Background
Cell culture refers to a method of simulating the in vivo environment in vitro, allowing it to survive, grow, reproduce and maintain the main structure and function. The culture of the cells has great significance to modern science and technology, and is beneficial to new progress in medical technology by exploring the cell culture.
Cell culture usually is in the culture flask adherent growth, and the culture environment of cell needs to advance under sterile environment to still need to give the cell and carry culture solution and take a breath, but present culture flask opens the opening when carrying the culture solution and when taking a breath, leads to dust and germ in the air to get into easily, leads to cell death, and the inside big opening of culture flask is little simultaneously, leads to wasing inconvenient.
SUMMERY OF THE UTILITY MODEL
Based on the above mentioned shortcomings in the prior art, the utility model provides a cell culture bottle which is not easy to pollute and is easy to identify.
The utility model discloses an adopt following technical scheme to overcome above technical problem, specifically do:
the utility model provides a cell culture bottle of easy discernment is difficult for polluting, include the blake bottle body, set up in the culture solution input port of blake bottle body one side and set up in the scavenge port on blake bottle body top, the inside filter screen that is provided with of scavenge port, the scavenge port top is rotated and is provided with and is used for hiding the closing cap of filter screen, closing cap one end with be provided with the bullet spiral-lock subassembly between scavenge port one side, be provided with the valve plug in the culture solution input port inner wall, the draw-in groove has been seted up on the culture solution input port inner wall, the draw-in groove with the valve plug cooperatees, the inner groove is inside to be provided with and is used for going up and down the control assembly of valve plug, blake bottle body one side is provided with turns round the lid.
As a further aspect of the present invention: the elastic buckle assembly comprises a connecting block fixed on one side of the bottom end of the sealing cover, a supporting rod is fixed on one side of the air vent, an adapter rod is arranged on the supporting rod in a rotating mode, and a first spring is arranged between one end of the adapter rod and the surface of the air vent.
As a further aspect of the present invention: the control assembly comprises a first thread groove arranged inside the valve plug, a threaded rod is inserted inside the inner groove, the threaded rod is in threaded connection with the first thread groove, a rotating knob is fixed at one end of the outer portion of the culture solution input port, a second spring is sleeved on one section of the surface inside the inner groove, and a limiting assembly is arranged on the inner wall of the inner groove.
As a further aspect of the present invention: the limiting assembly comprises a sliding groove formed in the inner wall of the inner groove, a limiting block is sleeved in the sliding groove in a sliding mode, and one end of the limiting block is fixedly connected with the surface of the valve plug.
As a further aspect of the present invention: the novel culture bottle is characterized in that a threaded shaft is arranged on one side of the twist cover, a second threaded groove is formed in one end of the inner wall of the culture bottle body, the second threaded groove is in threaded connection with the threaded shaft, an annular groove is formed in the joint of the twist cover and the culture bottle body, and a sealing rubber ring is arranged inside the annular groove.
As a further aspect of the present invention: the inside wall of blake bottle body evenly is provided with latticed line.
After the structure more than adopting, the utility model discloses compare in prior art, possess following advantage: can shield the intraoral hole of culture solution input through valve plug lift operation and close, lead to outside dust to get into when preventing to this internal nutrient solution of carrying of blake bottle, lead to the cell death of cultivateing, rotate simultaneously through the closing cap and open and can take a breath to this internal portion of blake bottle, make the inside cell of blake bottle carry out aerobic respiration, increase activity to turn round the lid through the split and can make and wash this internal wall of blake bottle more convenient.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Fig. 2 is an enlarged schematic view of a point a in fig. 1 according to the present invention.
Fig. 3 is an enlarged schematic view of the point B in fig. 1 according to the present invention.
Fig. 4 is a three-dimensional view of the twist cover and the culture bottle body of the utility model.
In the figure: 1. a culture bottle body; 2. a culture solution input port; 3. a ventilation port; 4. a filter screen; 5. sealing the cover; 6. a valve plug; 7. a card slot; 8. an inner tank; 9. twisting a cover; 10. connecting blocks; 11. a strut; 12. a transfer lever; 13. a first spring; 14. a first thread groove; 15. a threaded rod; 16. turning; 17. a second spring; 18. a chute; 19. a limiting block; 20. a threaded shaft; 21. a second thread groove; 22. an annular groove; 23. and sealing the rubber ring.
Detailed Description
In order to facilitate understanding of the present invention, the present invention will be described more fully hereinafter with reference to the accompanying drawings. The preferred embodiments of the present invention are shown in the drawings. The invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
In addition, an element of the present invention may be said to be "secured to" or "disposed on" another element, either directly on the other element or with intervening elements present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only and do not represent the only embodiments.
Referring to fig. 1-4, in an embodiment of the present invention, a cell culture bottle with less contamination and easy recognition comprises a culture bottle body 1, a culture solution inlet 2 disposed at one side of the culture bottle body 1, and a ventilation port 3 disposed at the top end of the culture bottle body 1, a filter screen 4 is arranged inside the air exchange port 3, a sealing cover 5 for covering the filter screen 4 is rotatably arranged at the top end of the air exchange port 3, a spring buckle component is arranged between one end of the sealing cover 5 and one side of the air vent 3, a valve plug 6 is arranged in the inner wall of the culture solution input port 2, a clamping groove 7 is arranged on the inner wall of the culture solution input port 2, the clamping groove 7 is matched with the valve plug 6, an inner groove 8 is formed in the inner wall of the culture solution inlet 2, a control assembly for lifting the valve plug 6 is arranged in the inner groove 8, and a twist cover 9 is arranged on one side of the culture bottle body 1;
through after cultivateing the cell on 1 inner wall of blake bottle body, open closing cap 5 through the bullet knot subassembly, take a breath to 1 inside of blake bottle body, the cell is breathed through aerobic, external dust and germ get into when filter screen 4 can prevent to take a breath, open valve plug 6 through control assembly simultaneously, carry the culture solution to the cell of 1 inside of blake bottle body, reduce dust and germ and get into simultaneously, turn round lid 9 and unpack apart when cell culture accomplishes, the convenience is washd 1 inner wall of blake bottle body.
In an embodiment of the present invention, the elastic buckle assembly includes a connecting block 10 fixed on one side of the bottom end of the cover 5, a supporting rod 11 is fixed on one side of the ventilation port 3, an adapting rod 12 is rotatably arranged on the supporting rod 11, and a first spring 13 is arranged between one end of the adapting rod 12 and the surface of the ventilation port 3;
through pressing the one end of switching pole 12, the one end perk that switching pole 12 and connecting block 10 buckled mutually is loosened, and closing cap 5 then can rotate and open and take a breath, rotates when the completion of taking a breath and buckles closing cap 5, then loosens switching pole 12, and first spring 13 can be supported switching pole 12 and rotate, makes connecting block 10 being buckled to switching pole 12 one end, prevents that connecting block 10 from taking off.
In another embodiment of the present invention, the control component includes a first thread groove 14 opened inside the valve plug 6, a threaded rod 15 is inserted inside the inner groove 8, the threaded rod 15 is in threaded connection with the first thread groove 14, a knob 16 is fixed at one end of the threaded rod 15 outside the culture solution input port 2, a second spring 17 is sleeved on one section of the surface of the threaded rod 15 inside the inner groove 8, and a limit component is arranged on the inner wall of the inner groove 8;
support 6 one side of valve plug in 2 inside through inserting the transfer line earlier culture solution input ports, then turn round 16 through rotating and drive threaded rod 15, threaded rod 15 makes valve plug 6 can the lift operation in 14 inside spiral rotations of first thread groove, opens the back through valve plug 6 and carries the culture solution again, can reduce the pollution of outside air and dust.
In another embodiment of the present invention, the limiting component includes a sliding groove 18 opened on the inner wall of the inner groove 8, a limiting block 19 is slidably sleeved inside the sliding groove 18, and one end of the limiting block 19 is fixedly connected with the surface of the valve plug 6;
the limiting block 19 can prevent the valve plug 6 from shaking in the lifting process when sliding in the sliding groove 18.
In another embodiment of the present invention, a threaded shaft 20 is disposed on one side of the twist cap 9, a second threaded groove 21 is disposed on one end of the inner wall of the culture bottle body 1, the second threaded groove 21 is in threaded connection with the threaded shaft 20, an annular groove 22 is disposed at the connection between the twist cap 9 and the culture bottle body 1, and a sealing rubber ring 23 is disposed inside the annular groove 22;
can follow culture flask body 1 one side through turning round lid 9 and tear open, conveniently wash culture flask body 1 inner wall.
In another embodiment of the present invention, the inner side wall of the culture bottle body 1 is uniformly provided with grid-shaped lines;
the inside latticed line of blake bottle body 1 can increase the hydrophobicity, is favorable to the cell adherent growth, can the effectual space growth that utilizes blake bottle body 1 inner wall.
The foregoing is illustrative of the preferred embodiments of the present invention only, and is not to be construed as limiting the claims. The present invention is not limited to the above embodiments, and the specific structure thereof is allowed to be changed. However, all changes which come within the scope of the independent claims of the invention are to be embraced therein.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

Claims (6)

1. The cell culture bottle is not easy to pollute and easy to identify, comprises a culture bottle body (1), a culture solution input port (2) arranged on one side of the culture bottle body (1) and a ventilation port (3) arranged on the top end of the culture bottle body (1), and is characterized in that a filter screen (4) is arranged inside the ventilation port (3), a sealing cover (5) used for covering the filter screen (4) is rotatably arranged on the top end of the ventilation port (3), an elastic buckle component is arranged between one end of the sealing cover (5) and one side of the ventilation port (3), a valve plug (6) is arranged in the inner wall of the culture solution input port (2), a clamping groove (7) is formed in the inner wall of the culture solution input port (2), the clamping groove (7) is matched with the valve plug (6), and an inner groove (8) is formed in the inner wall of the culture solution input port (2), the internal groove (8) is internally provided with a control component used for lifting the valve plug (6), and one side of the culture bottle body (1) is provided with a twist cover (9).
2. The cell culture bottle not easy to contaminate and easy to identify according to claim 1, wherein the snap-on assembly comprises a connecting block (10) fixed on one side of the bottom end of the cover (5), a support rod (11) is fixed on one side of the vent (3), an adapter rod (12) is rotatably arranged on the support rod (11), and a first spring (13) is arranged between one end of the adapter rod (12) and the surface of the vent (3).
3. The cell culture bottle not easy to pollute and easy to identify according to claim 1, wherein the control component comprises a first thread groove (14) arranged inside the valve plug (6), a threaded rod (15) is inserted inside the inner groove (8), the threaded rod (15) is in threaded connection with the first thread groove (14), a knob (16) is fixed at one end of the threaded rod (15) outside the culture solution inlet (2), a second spring (17) is sleeved on one section of the surface of the threaded rod (15) inside the inner groove (8), and a limiting component is arranged on the inner wall of the inner groove (8).
4. The cell culture bottle with characteristics of difficult contamination and easy identification as claimed in claim 3, wherein the limiting component comprises a sliding groove (18) formed on the inner wall of the inner groove (8), a limiting block (19) is slidably sleeved inside the sliding groove (18), and one end of the limiting block (19) is fixedly connected with the surface of the valve plug (6).
5. The cell culture bottle not easy to pollute and easy to identify according to claim 1, wherein a threaded shaft (20) is arranged on one side of the twist cap (9), a second threaded groove (21) is formed at one end of the inner wall of the culture bottle body (1), the second threaded groove (21) is in threaded connection with the threaded shaft (20), an annular groove (22) is formed at the joint of the twist cap (9) and the culture bottle body (1), and a sealing rubber ring (23) is arranged inside the annular groove (22).
6. The cell culture bottle not easy to pollute and easy to identify according to claim 1, characterized in that the inner side wall of the culture bottle body (1) is uniformly provided with grid-shaped lines.
CN202022383294.4U 2020-10-23 2020-10-23 Cell culture bottle difficult to pollute and easy to identify Expired - Fee Related CN213739524U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202022383294.4U CN213739524U (en) 2020-10-23 2020-10-23 Cell culture bottle difficult to pollute and easy to identify

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202022383294.4U CN213739524U (en) 2020-10-23 2020-10-23 Cell culture bottle difficult to pollute and easy to identify

Publications (1)

Publication Number Publication Date
CN213739524U true CN213739524U (en) 2021-07-20

Family

ID=76852434

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202022383294.4U Expired - Fee Related CN213739524U (en) 2020-10-23 2020-10-23 Cell culture bottle difficult to pollute and easy to identify

Country Status (1)

Country Link
CN (1) CN213739524U (en)

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GR01 Patent grant
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CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20210720

CF01 Termination of patent right due to non-payment of annual fee