CN213275532U - Experimental device for be used for detecting air flue smooth muscle tension - Google Patents
Experimental device for be used for detecting air flue smooth muscle tension Download PDFInfo
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- CN213275532U CN213275532U CN202021584880.9U CN202021584880U CN213275532U CN 213275532 U CN213275532 U CN 213275532U CN 202021584880 U CN202021584880 U CN 202021584880U CN 213275532 U CN213275532 U CN 213275532U
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Abstract
The utility model provides an experimental apparatus for be used for detecting tensile air flue smooth muscle, including the iron stand platform, the iron stand platform is by supreme fixed support pole, L type glass stick and the tension transducer of being equipped with in proper order down, the other end of fixed support pole is equipped with double-deck wheat bath ware, the inside of double-deck wheat bath ware is equipped with the trachea strip, the bottom of trachea strip is connected with L type glass stick, the top of trachea strip is connected with the tension transducer, the tension transducer is connected with computer system, one side of iron stand platform is equipped with heating cycle basin and gas holder. Compared with the prior art, the method and the device for detecting the airway smooth muscle of the experimental animal are provided; the design of the tension transducer, the MedLab biological signal acquisition and processing system and the computer system improves the sensitivity of the equipment and the accuracy of data; the use of the heating circulating water tank, the double-layer Maifang bath vessel and the gas storage tank enhances the sustainability of the experimental process.
Description
[ technical field ] A method for producing a semiconductor device
The utility model relates to an experimental apparatus technical field, in particular to an experimental apparatus for be used for detecting air flue smooth muscle tension.
[ background of the invention ]
Asthma, a chronic respiratory disease, is increasingly becoming a major health hazard to humans. In developed western countries, the incidence of asthma is as high as 8% to 20% of the general population. Statistically, over 2 million people in the united states suffer from asthma, and over 5 thousand children die of asthma each year. The situation is substantially the same in other western countries. Of further concern is the rising incidence of asthma at a rate of 20% to 25% increase every 10 years, especially in developing countries, including china. Chinese asthma patients are estimated to be about 3000 thousands at present. Nevertheless, the etiology and mechanism of progression of asthma has not been clearly explained to date. Known important pathological causes relate to various aspects such as individual constitutions and environmental factors. Asthma is a biomechanical disease in the sense that there may be no necessary causal relationship between airway hyperresponsiveness and airway inflammation factors. In fact, if the mechanical-biological properties of airway smooth muscle are not pathologically changed, all other pathological factors are not fatal. The lungs are involved in all the stages of gas exchange, the basic vital function. Thus, the biomechanical behavior of airway smooth muscle plays an important role in the pathogenesis of asthma.
During breathing, the flow of gas within the airway is directly related to the airway caliber. Although in some pathological conditions, such as asthma, chronic obstructive emphysema, the thickness of the airway mucosa and secretions of a patient are important factors influencing the caliber of the airway, it is generally considered that the tension change of the ASM is still the main reason for determining the caliber of the airway.
ASM is very sensitive to changes in the surrounding physical environment, and abnormal mechanical stimuli may cause changes in the structure or function of airway smooth muscle cells, which in turn causes symptoms such as excessive contraction of airway smooth muscle. In recent years, a great deal of research has been conducted on the biomechanical behavior of airway smooth muscle and its role in the pathological mechanism of asthma, and many important findings have been obtained, mainly including: the contraction of airway smooth muscle, the tissue structure of skeleton fiber, the functional length range and adaptability of airway smooth muscle, the change of airway smooth muscle cell structure and function caused by mechanical stimulation, the regulation and control of airway smooth muscle tension to the response of strain induction, the dynamic behavior of cytoskeleton soft vitreous state and the like. Therefore, in the scientific fields of clinical medicine, scientific experiments, teaching experiments, drug research and the like, the strain of the ASM, particularly the strain of the ASM of an experimental animal, is often required to be detected so as to judge whether a certain stimulus or a certain drug is effective. Such as: when developing a specific asthma relieving medicine, whether the main component of the medicine can rapidly dilate the trachea or not is often required to be detected.
The existing muscle tension detection device has certain instability in the detection process, and is particularly used for detecting in-vitro local tissue muscles. At present, most of muscle tension detection devices are designed for human body application, and devices for detecting the muscle tension of experimental animals applied to the scientific field are very few, but the devices for detecting the airway smooth muscle tension of the experimental animals are less.
The following patent documents relevant to the present application are found by search, and specific disclosures are as follows:
1. the utility model provides a spasm sensor (CN108433735A) based on muscle tension detects, including the muscle power transmission module that is used for gathering human surface muscle tension, adjust the quick unit module of force of muscle power transmission module and human muscle contact dynamics, receive and handle sensor data and finally show the detection module of spasm characteristic information, a casing module for installing muscle power transmission module, quick unit module of force and detection module, muscle power transmission module connects the lower extreme at casing module, quick unit module of force and detection module are located the upper end of muscle power transmission module and are linked to each other with casing module's upper end. The method can stably obtain the amplitude and frequency of the muscle tension, evaluate the spasm characteristic information from the perspective of muscle tension perception, and realize quantitative evaluation of the spasm; the spasm sensor has small volume and light weight, and is convenient for clinical measurement of rehabilitation.
2. A muscle tension detection and analysis device and a detection method (CN1077026A) are provided, the muscle tension detection and analysis device is composed of a plurality of modules such as a transmission device, a force measuring device, a resistance detection device and an amplification module, the force measuring device is connected with a force sensor through a sliding frame in the force measuring device, and a skeleton support is communicated with an output shaft, so that the condition of muscle tension can be directly, quantitatively and qualitatively detected.
3. An animal muscle tone detection device (CN203597957U) comprising a data acquisition and processing system and a force detection system, the force detection system comprising: the support is provided with at least one support column; the force sensing device is connected to the supporting column and is used for acquiring muscle tension data; the force sensing device comprises a force sensor, a muscle connector and an inelastic guide wire connected between the force sensor and the muscle connector; the force sensor and the muscle connector are respectively connected with the data acquisition and processing system and the sample to be measured, and the guide line is kept perpendicular to the force measuring surface of the force sensor. The utility model discloses simple structure, the equipment cost is low, convenient operation, the accuracy is high, can the wide application detect the different position's of different sizes animal muscle tension.
Through retrieval, the existing methods and patents for detecting the airway smooth muscle tension of experimental animals are few, and the detection and rehabilitation purposes for human are more. Through the contrast of technical characteristics, above-mentioned patent literature that discloses all is different with the utility model discloses, and technical structure also is different, can not influence the creativity and the novelty of the utility model application.
[ Utility model ] content
An object of the utility model is to provide an experimental apparatus for be used for detecting air flue smooth muscle tension to overcome the muscle tension detection device among the prior art and have certain technical problem such as instability in the testing process.
In order to achieve the above object, the utility model provides a following technical scheme:
the application discloses an experimental device for detecting airway smooth muscle tension, which comprises an iron stand, a double-layer Maifang bath vessel, an air pipe, an L-shaped glass rod, a tension transducer, a heating circulating water tank, an air storage tank and a computer system, the iron support is provided with a fixed support rod, an L-shaped glass rod and a tension transducer from bottom to top in sequence, the other end of the fixed supporting rod is provided with a double-layer Maifang bath vessel, an air pipe is arranged inside the double-layer Maifang bath vessel, the bottom of the air pipe is connected with an L-shaped glass rod, the top of the air pipe is connected with a tension transducer, the tension transducer is connected with a computer system, one side of the iron stand is provided with a heating circulating water tank and an air storage tank, the heating circulating water tank is connected with the double-layer Maishi bath utensil through a water pipe, a gas outlet thin pipe is arranged on the gas storage tank, and a gas outlet of the gas outlet thin pipe is located under the gas pipe.
Preferably, an S-shaped bent pipe is arranged at one end, away from the iron support, of the L-shaped glass rod, and the lower end of the air pipe is connected with the S-shaped bent pipe through a thin line.
Preferably, the detection end of the tension transducer is connected with the tracheal strip through a fishhook.
Preferably, the signal output end of the tension transducer is connected with the signal input end of a MedLab biological signal acquisition and processing system, and the signal output end of the MedLab biological signal acquisition and processing system is connected with the signal input end of the computer system.
Preferably, the air outlet tubule is provided with an air outlet valve, and the part of the air outlet tubule, which is positioned inside the double-layer Maifang bath vessel, is attached to the inner wall of the double-layer Maifang bath vessel.
Preferably, 95% of O is supplied into the gas storage tank2And 5% CO2The mixed gas of (1).
The utility model has the advantages that:
(1) the utility model has strong pertinence and provides a whole set of method and device for detecting the airway smooth muscle of an experimental animal;
(2) the design of the tension transducer, the MedLab biological signal acquisition and processing system and the computer system changes the prior lever-marbling drum tracings into the scanning of a transducer-recorder device, greatly improves the sensitivity of the equipment and the accuracy of data, and reduces the use of samples such as trachea sheets, trachea rings and the like;
(3) the device has visualized data information, can record and observe the tension change condition of airway smooth muscle in real time, and provides convenience for the adjustment of the experimental process;
(4) the use of the heating circulating water tank, the double-layer Maifang bath vessel and the gas storage tank provides guarantee for the activity maintenance of the airway smooth muscle after the separation, and the sustainability of the experimental process is enhanced.
(5) The utility model discloses a MedLab biological signal acquisition and processing system who adopts has a plurality of passageways, can carry out the tensile detection of a plurality of air flue smooth muscle simultaneously, and the going on of convenient experiment improves experimental efficiency.
The features and advantages of the present invention will be described in detail by embodiments with reference to the accompanying drawings.
[ description of the drawings ]
FIG. 1 is a schematic structural diagram of an experimental apparatus for detecting airway smooth muscle tension according to the present invention;
in the figure: the device comprises a 1-iron stand, a 2-double-layer Mains bath, a 21-fixed support rod, a 3-air pipe, a 4-L-shaped glass rod, a 41-S-shaped bent pipe, a 5-tension transducer, a 6-heating circulating water tank, a 61-water pipe, a 7-computer system, an 8-air storage tank, an 81-air outlet thin pipe, an 82-air outlet valve and a 9-MedLab biological signal acquisition and processing system.
[ detailed description ] embodiments
In order to make the objects, technical solutions and advantages of the present invention more clear, the present invention is further described in detail through the accompanying drawings and embodiments. It should be understood, however, that the description herein of specific embodiments is only intended to illustrate the invention and not to limit the scope of the invention. Moreover, in the following description, descriptions of well-known structures and techniques are omitted so as to not unnecessarily obscure the concepts of the present invention.
Referring to fig. 1, an embodiment of the present invention provides an experimental apparatus for detecting airway smooth muscle tension, including an iron support 1, a double-layer maishi bath vessel 2, an air tube 3, an L-shaped glass rod 4, a tension transducer 5, a heating circulation water tank 6, an air storage tank 8 and a computer system 7, wherein the iron support 1 is sequentially provided with a fixed support rod 21, an L-shaped glass rod 4 and a tension transducer 5 from bottom to top, the other end of the fixed support rod 21 is provided with the double-layer maishi bath vessel 2, the air tube 3 is arranged inside the double-layer maishi bath vessel 2, the bottom of the air tube 3 is connected with the L-shaped glass rod 4, the top of the air tube 3 is connected with the tension transducer 5, the tension transducer 5 is connected with the computer system 7, one side of the iron support 1 is provided with a heating circulation water tank 6 and an air storage tank 8, the heating circulation water tank 6 is connected with the double-layer maishi bath vessel 2 through a water, be equipped with out tubule 81 on the gas holder 8, the gas outlet of giving vent to anger tubule 81 is located under the trachea strip 3.
And one end of the L-shaped glass rod 4, which is far away from the iron stand 1, is provided with an S-shaped bent pipe 41, and the lower end of the air pipe 3 is connected with the S-shaped bent pipe 41 through a thin line. The detection end of the tension transducer 5 is connected with the tracheal strip 3 through a fishhook. The signal output end of the tension transducer 5 is connected with the signal input end of a MedLab biological signal acquisition and processing system 9, and the signal output end of the MedLab biological signal acquisition and processing system 9 is connected with the signal input end of the computer system 7. The tension change of the tension transducer 5 is converted into an electric signal, the electric signal is output to the MedLab biological signal acquisition and processing system 9, the information is transmitted to the computer system 7 by the MedLab biological signal acquisition and processing system 9, and the data is recorded and displayed in real time.
The above-mentionedAn air outlet valve 82 is arranged on the air outlet tubule 81, and the part of the air outlet tubule 81, which is positioned inside the double-layer Maifang bath vessel 2, is attached to the inner wall of the double-layer Maifang bath vessel 2. 95% of O is supplied in the gas storage tank 82And 5% CO2The mixed gas of (1).
The utility model discloses the working process:
(1) preparing trachea strips, taking 1 animal, cutting the neck skin from the middle of the ventral surface immediately after euthanasia, stripping subcutaneous tissues, carefully separating an air outlet pipe, cutting all the trachea from the lower part of the thyroid cartilage, putting the trachea strips into a flat dish containing the Kjeldahl solution, and cutting off connective tissues around the trachea. Then longitudinally cutting the ventral surface (cartilage ring surface) of the trachea, transversely cutting the trachea at intervals of 2-3 cartilage rings, equally dividing the removed trachea into 5-6 sections, sewing each trachea at the longitudinal cut by using a needle and a thread, and connecting the trachea into a string to obtain a trachea strip 3;
(2) one end of the air pipe 3 is connected with a fishhook connected with a tension transducer 5 through a thin wire, the other end of the air pipe is connected with an S-shaped bent pipe 41 of an L-shaped glass rod 4 through a thin wire, the whole air pipe 3 is fixed in a double-layer Maifanshi bath vessel 2 containing a Ke-Heng' S nutrient solution, and the temperature of the air pipe is kept constant at 37 ℃ through a heating circulating water tank 6 and a water pipe 61; inserting an air outlet tubule 81 of the air storage tank 8 into the bottom of the double-layer Maifang bath utensil 2 along the inner edge of the double-layer Maifang bath utensil, and adjusting an air outlet valve 82 to enable the air outlet tubule 81 to generate bubbles at a proper speed, ensure that the generated bubbles just pass through the air pipe 3 to generate action, and keep the activity of airway smooth muscle;
(3) adjust the preload and start the balance. During the balance period, changing the liquid for 1 time every 15min, changing the liquid for 4 times in total, performing pre-stimulation by using high-potassium-concentration gram-sharehi nutrient solution after the balance is finished, changing the liquid for elution after the activity of the tracheal strips 3 is fully stimulated, recovering to a base line, and starting the test after 30 min;
(4) stimulating again by using high potassium concentration gram-Xiang's nutrient solution, and then starting the experiment;
(5) the drug to be tested is added according to the concentration gradient, and whether the pre-contracted tracheal tube 3 is relaxed or not is observed. The observation time is generally 2-5 min, and the tension change condition before and after administration is observed;
(6) comparing the change conditions of baseline, contractility after high potassium stimulation and tension after administration, and determining the concentration of the drug to be tested in the relaxation of airway smooth muscle and maximum relaxation
(7) The tracheal strip 3 was removed and the experiment was completed.
The above description is only exemplary of the present invention and should not be taken as limiting the scope of the present invention, as any modification, equivalent replacement or improvement made within the spirit and principle of the present invention should be included in the present invention.
Claims (6)
1. An experimental device for detecting airway smooth muscle tension is characterized in that: comprises an iron stand platform (1), a double-layer Maishi bath vessel (2), an air pipe (3), an L-shaped glass rod (4), a tension transducer (5), a heating circulating water tank (6), an air storage tank (8) and a computer system (7), wherein the iron stand platform (1) is sequentially provided with a fixed support rod (21), the L-shaped glass rod (4) and the tension transducer (5) from bottom to top, the other end of the fixed support rod (21) is provided with the double-layer Maishi bath vessel (2), the air pipe (3) is arranged inside the double-layer Maishi bath vessel (2), the bottom of the air pipe (3) is connected with the L-shaped glass rod (4), the top of the air pipe (3) is connected with the tension transducer (5), the tension transducer (5) is connected with the computer system (7), one side of the iron stand platform (1) is provided with the heating circulating water tank (6) and the air storage tank (8), heating cycle water tank (6) and double-deck maishi bath ware (2) between be connected through water pipe (61), be equipped with out gas tubule (81) on gas holder (8), the gas outlet of giving vent to anger tubule (81) is located under trachea strip (3).
2. The experimental apparatus for measuring airway smooth muscle tone of claim 1, wherein: one end of the L-shaped glass rod (4) far away from the iron support (1) is provided with an S-shaped bent pipe (41), and the lower end of the air pipe (3) is connected with the S-shaped bent pipe (41) through a thin line.
3. The experimental apparatus for measuring airway smooth muscle tone of claim 1, wherein: the detection end of the tension transducer (5) is connected with the tracheal strip (3) through a fishhook.
4. The experimental apparatus for measuring airway smooth muscle tone of claim 1, wherein: the signal output end of the tension transducer (5) is connected with the signal input end of a MedLab biological signal acquisition and processing system (9), and the signal output end of the MedLab biological signal acquisition and processing system (9) is connected with the signal input end of the computer system (7).
5. The experimental apparatus for measuring airway smooth muscle tone of claim 1, wherein: be equipped with air outlet valve (82) on the tubule of giving vent to anger (81), the tubule of giving vent to anger (81) are located the inside part of double-deck wheat bath ware (2) and paste mutually with the inner wall of double-deck wheat bath ware (2).
6. The experimental apparatus for measuring airway smooth muscle tone of claim 1, wherein: 95% of O is supplied in the gas storage tank (8)2And 5% CO2The mixed gas of (1).
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| CN202021584880.9U CN213275532U (en) | 2020-08-04 | 2020-08-04 | Experimental device for be used for detecting air flue smooth muscle tension |
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| CN202021584880.9U CN213275532U (en) | 2020-08-04 | 2020-08-04 | Experimental device for be used for detecting air flue smooth muscle tension |
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