CN212925033U - Hog cholera double-fluorescence PCR detection kit - Google Patents

Hog cholera double-fluorescence PCR detection kit Download PDF

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Publication number
CN212925033U
CN212925033U CN202021622922.3U CN202021622922U CN212925033U CN 212925033 U CN212925033 U CN 212925033U CN 202021622922 U CN202021622922 U CN 202021622922U CN 212925033 U CN212925033 U CN 212925033U
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China
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box
test tube
tube fixing
dry ice
pipe
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CN202021622922.3U
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Chinese (zh)
Inventor
刘近
鲁龙
杨转
梁静
石书霞
陈焕娇
姬杰菲
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Hexu Zhengzhou Biotechnology Co ltd
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Hexu Zhengzhou Biotechnology Co ltd
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Abstract

The utility model provides a hog cholera dual fluorescence PCR detect reagent box, includes box body (1) and lid (2), its characterized in that: the utility model discloses a test tube, including box body (1), inner wall and/or outer wall, the inner wall of box body (1) is seted up hollow vacuum intermediate layer (5) in the inner wall of box body (1), and the inner chamber bottom of box body (1) sets up to dry ice chamber (14), and fixed being provided with the round on the inner wall of box body (1) and shelving boss (6), shelve on boss (6) and shelved test tube fixing base (8), and the bottom of test tube fixing base (8) just is corresponding to test tube fixed orifices (9) respectively the vertical fixation have heat pipe (10), the utility model discloses it is rational in infrastructure, even the detection kit still can keep lower temperature for a long time after breaking away from refrigeration equipment, inside keeps warm and insulates against heat effectually to it is good to detect the sealing performance that detects the box, is difficult to be.

Description

Hog cholera double-fluorescence PCR detection kit
Technical Field
The utility model relates to a detect reagent box especially relates to a dual fluorescence PCR detect reagent box of swine fever, belongs to animal epidemic disease detection device technical field.
Background
The African swine fever is a swine disease caused by acute and highly infectious filterable virus, the death rate is up to 100 percent, the African swine fever virus can be proved from blood, tissue fluid, internal organs and other excreta of infected pigs, and the virus existing in the blood in a low-temperature dark room can survive for six years. If swine fever occurs, the test substance needs to be extracted quickly and sent for inspection, once the African swine fever virus is confirmed, the African swine fever virus is isolated, killed and harmlessly treated, and according to the characteristics of strong infectivity and fear of light and high temperature of the African swine fever virus, the collected test substance needs to be placed in a sealed low-temperature environment for inspection, so that the accuracy of the test result is ensured. The detection box is simple in structure, after the detection box is separated from refrigeration equipment, the detection box cannot automatically maintain the internal low-temperature environment for a long time, in addition, the existing detection box is poor in sealing performance, the test result is inaccurate easily, serious loss is easily caused, and therefore a convenient collection box with low-temperature sealing is lacked for inspection.
Disclosure of Invention
The utility model aims at current classical swine fever virus detect reagent box simple structure, after breaking away from the refrigeration plant, detect and detect the unable long time of box and maintain inside low temperature environment by oneself, and the sealing performance who detects the box is not good moreover, contaminated defect and not enough easily, it is rational in infrastructure now to provide one kind, even detect the reagent box still can keep lower temperature for a long time after breaking away from the refrigeration plant, inside keeps warm and insulates against heat effectually, and the sealing performance who detects the box is good, be difficult to be contaminated, the dual fluorescence PCR detect reagent box of classical swine fever of very convenient to use.
In order to achieve the above object, the technical solution of the present invention is: the utility model provides a dual fluorescence PCR detect reagent box of hog cholera, includes box body and lid, and the lid, its characterized in that are installed through the connecting axle to the upper end one side of box body: the inner wall of the box body is provided with a hollow vacuum interlayer, the surface of the inner wall and/or the outer wall of the box body is provided with a heat insulation layer, the bottom of the inner cavity of the box body is provided with a dry ice cavity, dry ice is placed in the dry ice cavity, a circle of placing boss is fixedly arranged on an upper port of the inner wall of the box body, a test tube fixing seat is placed on the placing boss, a circle of bonding and fixing layer is arranged between the placing boss and the test tube fixing seat, the upper surface of the test tube fixing seat is uniformly provided with a plurality of test tube fixing holes, the bottom of the test tube fixing seat is respectively and vertically fixed with a heat conduction tube corresponding to the test tube fixing holes, the upper end of the heat conduction tube is of an open structure, the lower end of the heat conduction tube is of a closed structure, the lower part of the, the air inlet end of the one-way exhaust valve is communicated with the dry ice cavity, and the air outlet end of the one-way exhaust valve extends out of the box body.
Furthermore, a circle of sealing ring corresponding to the upper port of the box body or the upper port of the test tube fixing seat is arranged on the inner side surface of the box cover.
Furthermore, a box cover fixing buckle is arranged on the box cover, and a lock catch matched with the box cover fixing buckle is arranged on the outer wall of the box body.
Furthermore, the heat conduction pipe is made of a thin metal pipe or a plastic pipe, and the diameter of the pipe orifice at the upper end of the heat conduction pipe is smaller than or equal to the aperture of the test tube fixing hole.
Further, the inside of heat pipe is fixed with the elasticity spacing ring, and the elasticity spacing ring adopts elasticity foam or elasticity rubber to make cyclic annular structure, and the interior circle diameter of elasticity spacing ring is less than the external diameter of test tube.
The utility model has the advantages that:
1. the utility model discloses be provided with heat preservation insulating layer and vacuum intermediate layer, reached fine heat preservation thermal-insulated effect to set up to the dry ice chamber in the inner chamber bottom of box body, and be provided with the heat pipe that the heat conduction is respond well, reduce the temperature in the reagent box through the dry ice chamber.
2. The utility model discloses it is rational in infrastructure, detect reagent box even still can keep lower temperature for a long time after breaking away from refrigeration plant, and inside keeps warm thermal-insulated effectual to the sealing performance that detects the detection box is good, is difficult to be contaminated, and it is very convenient to use.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a schematic structural view of the present invention when no test tube is placed.
In the figure: the test tube box comprises a box body 1, a box cover 2, a connecting shaft 3, a heat preservation and insulation layer 4, a vacuum interlayer 5, a shelving boss 6, a bonding and fixing layer 7, a test tube fixing seat 8, a test tube placing hole 9, a heat conduction pipe 10, a test tube 11, an elastic limiting ring 12, a sealing limiting plug 13, a dry ice cavity 14, a box cover fixing buckle 15, a one-way exhaust valve 16, dry ice 17 and a sealing ring 18.
Detailed Description
The invention is described in further detail below with reference to the following description of the drawings and the detailed description.
Referring to fig. 1 and 2, the utility model discloses a dual fluorescence PCR detect reagent box of swine fever, including box body 1 and lid 2, its characterized in that are installed through connecting axle 3 to upper end one side of box body 1: a hollow vacuum interlayer 5 is arranged in the inner wall of the box body 1, a heat insulation layer 4 is arranged on the surface of the inner wall and/or the outer wall of the box body 1, a dry ice cavity 14 is arranged at the bottom of the inner cavity of the box body 1, dry ice 17 is placed in the dry ice cavity 14, a circle of placing boss 6 is fixedly arranged on the upper port of the inner wall of the box body 1, a test tube fixing seat 8 is placed on the placing boss 6, a circle of bonding fixing layer 7 is arranged between the placing boss 6 and the test tube fixing seat 8, a plurality of test tube fixing holes 9 are uniformly formed in the upper surface of the test tube fixing seat 8, heat conduction pipes 10 are respectively and vertically fixed at the bottom of the test tube fixing seat 8 and corresponding to the test tube fixing holes 9, the upper ends of the heat conduction pipes 10 are of an open structure, the lower ends of the heat conduction pipes 10, be provided with sealed stopper 13 on the last port of test tube 11, install one-way discharge valve 16 on the lateral wall of box body 1, the inlet end and the dry ice chamber 14 of one-way discharge valve 16 are linked together, and the end of giving vent to anger of one-way discharge valve 16 stretches out the outside of box body 1.
And a circle of sealing ring 18 corresponding to the upper port of the box body 1 or the upper port of the test tube fixing seat 8 is arranged on the inner side surface of the box cover 2.
The box cover 2 is provided with a box cover fixing buckle 15, and the outer wall of the box body 1 is provided with a lock catch matched with the box cover fixing buckle 15.
The heat conducting pipe 10 is made of a thin metal pipe or a plastic pipe, and the diameter of the pipe orifice at the upper end of the heat conducting pipe 10 is smaller than or equal to the aperture of the test tube fixing hole 9.
The inside of heat pipe 10 is fixed with elasticity spacing ring 12, and elasticity spacing ring 12 adopts elasticity foam or elasticity rubber to make annular structure, and the interior circle diameter of elasticity spacing ring 12 is less than the external diameter of test tube 11.
As shown in the attached drawings 1 to 2, a box cover 2 is installed on one side of the upper end of a box body 1 through a connecting shaft 3, a box cover fixing buckle 15 is arranged on the box cover 2, and a lock catch matched with the box cover fixing buckle 15 is arranged on the outer wall of the box body 1. A hollow vacuum interlayer 5 is arranged in the inner wall of the box body 1, and the vacuum interlayer 5 can play a good role in heat preservation and heat insulation for the interior of the box body 1. In addition, a heat insulation layer 4 is arranged on the surface of the inner wall and/or the outer wall of the box body 1, and the heat insulation layer 4 is made of an extruded polystyrene board or a thin-layer heat insulation board, so that the heat insulation effect is further improved, and a good low-temperature environment is created in the box body 1. Simultaneously, be provided with sealing washer 18 on the medial surface of lid 2, sealing washer 18 is corresponding with the last port of box body 1 or the last port of test tube fixing base 8, and sealing washer 18 contacts and realizes the extrusion of certain degree with box body 1 or test tube fixing base 8 after lid 2 covers to reach better sealed effect.
The bottom of the inner cavity of the box body 1 is provided with a dry ice cavity 14, dry ice 17 is placed in the dry ice cavity 14, the dry ice 17 can absorb a large amount of heat in the gradual vaporization process, the temperature is reduced, the temperature in the dry ice cavity 14 is lower, and the principle is utilized to store medicines, reagents and even detection samples at low temperature. The fixed boss 6 of shelving of round that is provided with on the inner wall of box body 1 port, shelve and shelve test tube fixing base 8 on the boss 6, shelve and be provided with round bonding fixed layer 7 between boss 6 and the test tube fixing base 8, test tube fixing base 8 seals and fixes on shelving the boss 6 through bonding fixed layer 7, when needs take out test tube fixing base 8, exerts certain power and just can make and shelve the separation between boss 6 and the test tube fixing base 8.
A plurality of test tube fixed orifices 9 have evenly been seted up to test tube fixing base 8's upper surface, and test tube fixing base 8's bottom just is fixed with heat pipe 10 corresponding to test tube fixed orifices 9 respectively vertically, and heat pipe 10 adopts thin pipe of metal or plastic tubing to make, and heat pipe 10's heat conduction effect is fine, and the upper end mouth of pipe diameter of heat pipe 10 is less than or equal to the aperture of test tube fixed orifices 9. The upper end of the heat conduction pipe 10 is open structure, the lower end of the heat conduction pipe 10 is closed structure, and the lower part of the heat conduction pipe 10 is located in the dry ice chamber 14. Test tube 11 has been placed to the interpolation in test tube fixed orifices 9 and the heat pipe 10, and test tube 11 can keep unanimous with the temperature in the dry ice chamber 14 under the effect of heat pipe 10 for test tube 11 is in lower temperature. In order to realize the sealing of the test tube 11 and avoid the upward separation of the test tube 11 during transportation, a sealing limit plug 13 is arranged on the upper end opening of the test tube 11. In addition, an elastic limiting ring 12 is fixed inside the heat conducting pipe 10, the elastic limiting ring 12 is made of elastic foam or elastic rubber and is of an annular structure, and the diameter of the inner circle of the elastic limiting ring 12 is smaller than the outer diameter of the test tube 11. The elastic limiting ring 12 is used for supporting and fixing the lower section of the test tube 11, and prevents the test tube 11 from being damaged due to collision between the test tube 11 and the elastic limiting ring 12.
In addition, in order to exhaust the vaporized dry ice in the dry ice cavity 14, a one-way exhaust valve 16 is installed on the side wall of the box body 1, the air inlet end of the one-way exhaust valve 16 is communicated with the dry ice cavity 14, the air outlet end of the one-way exhaust valve 16 extends out of the outer side of the box body 1, the one-way exhaust valve 16 conducts one-way exhaust, redundant air in the dry ice cavity 14 is exhausted, and the test tube fixing seat 8 is prevented from being expanded due to overhigh air pressure in the dry ice cavity 14.
The above description is made for further details of the present invention with reference to the specific embodiments, and it should not be considered that the embodiments of the present invention are limited to these descriptions, and for those skilled in the art to which the present invention pertains, the simple replacement made without departing from the concept of the present invention should be considered as belonging to the protection scope of the present invention.

Claims (5)

1. The utility model provides a swine fever dual fluorescence PCR detect reagent box, includes box body (1) and lid (2), its characterized in that are installed through connecting axle (3) in the upper end one side of box body (1): a hollow vacuum interlayer (5) is arranged in the inner wall of the box body (1), a heat-insulating layer (4) is arranged on the surface of the inner wall and/or the outer wall of the box body (1), a dry ice cavity (14) is arranged at the bottom of the inner cavity of the box body (1), dry ice (17) is placed in the dry ice cavity (14), a circle of placing boss (6) is fixedly arranged on an upper port of the inner wall of the box body (1), a test tube fixing seat (8) is placed on the placing boss (6), a circle of bonding fixing layer (7) is arranged between the placing boss (6) and the test tube fixing seat (8), a plurality of test tube fixing holes (9) are uniformly formed in the upper surface of the test tube fixing seat (8), heat conduction pipes (10) are respectively and vertically fixed at the bottom of the test tube fixing seat (8) and corresponding to the test tube fixing holes (9), the upper ends of, the lower part of heat pipe (10) is located dry ice chamber (14), and test tube (11) have been placed in inserting in test tube fixed orifices (9) and heat pipe (10), are provided with sealed spacing stopper (13) on the last port of test tube (11), install one-way discharge valve (16) on the lateral wall of box body (1), and the inlet end and the dry ice chamber (14) of one-way discharge valve (16) are linked together, and the outside of box body (1) is stretched out to the end of giving vent to anger of one-way discharge valve (16).
2. The dual fluorescence PCR detection kit for swine fever according to claim 1, wherein the kit comprises: a circle of sealing ring (18) corresponding to the upper port of the box body (1) or the upper port of the test tube fixing seat (8) is arranged on the inner side surface of the box cover (2).
3. The dual fluorescence PCR detection kit for swine fever according to claim 1, wherein the kit comprises: the box cover (2) is provided with a box cover fixing buckle (15), and the outer wall of the box body (1) is provided with a lock catch matched with the box cover fixing buckle (15).
4. The dual fluorescence PCR detection kit for swine fever according to claim 1, wherein the kit comprises: the heat conduction pipe (10) is made of a thin metal pipe or a plastic pipe, and the diameter of the pipe orifice at the upper end of the heat conduction pipe (10) is smaller than or equal to the aperture of the test tube fixing hole (9).
5. The dual fluorescence PCR detection kit for swine fever according to claim 1, wherein the kit comprises: the inside of heat pipe (10) is fixed with elasticity spacing ring (12), and elasticity spacing ring (12) adopt elasticity foam or elasticity rubber to make cyclic annular structure, and the interior circle diameter of elasticity spacing ring (12) is less than the external diameter of test tube (11).
CN202021622922.3U 2020-08-07 2020-08-07 Hog cholera double-fluorescence PCR detection kit Active CN212925033U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202021622922.3U CN212925033U (en) 2020-08-07 2020-08-07 Hog cholera double-fluorescence PCR detection kit

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202021622922.3U CN212925033U (en) 2020-08-07 2020-08-07 Hog cholera double-fluorescence PCR detection kit

Publications (1)

Publication Number Publication Date
CN212925033U true CN212925033U (en) 2021-04-09

Family

ID=75335754

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202021622922.3U Active CN212925033U (en) 2020-08-07 2020-08-07 Hog cholera double-fluorescence PCR detection kit

Country Status (1)

Country Link
CN (1) CN212925033U (en)

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Address after: 450016 Jialin international, intersection of Fengyun Road, 15th Street, Zhengzhou area (Economic Development), Zhengzhou pilot Free Trade Zone, Zhengzhou City, Henan Province

Patentee after: Hexu (Zhengzhou) Biotechnology Co., Ltd

Address before: 450016 Fengyun Road, 15th Street, Guancheng District, Zhengzhou City, Henan Province

Patentee before: Hexu (Zhengzhou) Biotechnology Co., Ltd

CP02 Change in the address of a patent holder